schizosaccharomyces pombe proteins

Summary

Summary: Proteins obtained from the species Schizosaccharomyces pombe. The function of specific proteins from this organism are the subject of intense scientific interest and have been used to derive basic understanding of the functioning similar proteins in higher eukaryotes.

Top Publications

  1. pmc Comparative functional genomics of the fission yeasts
    Nicholas Rhind
    Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, 364 Plantation Street, Worcester, MA 01605, USA
    Science 332:930-6. 2011
  2. pmc RNAi-mediated targeting of heterochromatin by the RITS complex
    Andre Verdel
    Department of Cell Biology, Harvard Medical School, Boston, MA02115, USA
    Science 303:672-6. 2004
  3. pmc Polar opposites: Fine-tuning cytokinesis through SIN asymmetry
    Alyssa E Johnson
    Department of Cell and Developmental Biology, Vanderbilt University, Nashville, Tennessee, USA
    Cytoskeleton (Hoboken) 69:686-99. 2012
  4. pmc Assembly and architecture of precursor nodes during fission yeast cytokinesis
    Damien Laporte
    Department of Molecular Genetics, The Ohio State University, Columbus, OH 43210, USA
    J Cell Biol 192:1005-21. 2011
  5. ncbi Basic methods for fission yeast
    Susan L Forsburg
    Program in Molecular and Computational Biology, University of Southern California, 1050 Child s Way MCB201, Los Angeles, CA 90089 2910, USA
    Yeast 23:173-83. 2006
  6. ncbi Generating crossovers by resolution of nicked Holliday junctions: a role for Mus81-Eme1 in meiosis
    Fekret Osman
    Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, United Kingdom
    Mol Cell 12:761-74. 2003
  7. pmc CENP-A exceeds microtubule attachment sites in centromere clusters of both budding and fission yeast
    Valerie C Coffman
    Department of Molecular Genetics, The Ohio State University, Columbus, OH 43210, USA
    J Cell Biol 195:563-72. 2011
  8. ncbi Dynamic repertoire of a eukaryotic transcriptome surveyed at single-nucleotide resolution
    Brian T Wilhelm
    Cancer Research UK Fission Yeast Functional Genomics Group, Wellcome Trust Sanger Institute, Hinxton, Cambridge CB10 1HH, UK
    Nature 453:1239-43. 2008
  9. ncbi RNA elimination machinery targeting meiotic mRNAs promotes facultative heterochromatin formation
    Martin Zofall
    Laboratory of Biochemistry and Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Science 335:96-100. 2012
  10. ncbi Methylation of histone H4 lysine 20 controls recruitment of Crb2 to sites of DNA damage
    Steven L Sanders
    The Wellcome Trust Cancer Research UK Gurdon Institute and Department of Pathology, Tennis Court Road, Cambridge CB2 1QN, United Kingdom
    Cell 119:603-14. 2004

Detail Information

Publications276 found, 100 shown here

  1. pmc Comparative functional genomics of the fission yeasts
    Nicholas Rhind
    Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, 364 Plantation Street, Worcester, MA 01605, USA
    Science 332:930-6. 2011
    ..These analyses elucidate the genome structure and gene regulation of fission yeast and provide tools for investigation across the Schizosaccharomyces clade...
  2. pmc RNAi-mediated targeting of heterochromatin by the RITS complex
    Andre Verdel
    Department of Cell Biology, Harvard Medical School, Boston, MA02115, USA
    Science 303:672-6. 2004
    ..The results suggest a mechanism for the role of the RNAi machinery and small RNAs in targeting of heterochromatin complexes and epigenetic gene silencing at specific chromosomal loci...
  3. pmc Polar opposites: Fine-tuning cytokinesis through SIN asymmetry
    Alyssa E Johnson
    Department of Cell and Developmental Biology, Vanderbilt University, Nashville, Tennessee, USA
    Cytoskeleton (Hoboken) 69:686-99. 2012
    ..In this review, we highlight recent advances in our understanding of SIN regulation, with an emphasis on how SIN asymmetry is achieved and how this aspect of SIN regulation fine-tunes cytokinesis...
  4. pmc Assembly and architecture of precursor nodes during fission yeast cytokinesis
    Damien Laporte
    Department of Molecular Genetics, The Ohio State University, Columbus, OH 43210, USA
    J Cell Biol 192:1005-21. 2011
    ..Collectively, our work characterizing the assembly and architecture of precursor nodes defines important steps and molecular players for contractile ring assembly...
  5. ncbi Basic methods for fission yeast
    Susan L Forsburg
    Program in Molecular and Computational Biology, University of Southern California, 1050 Child s Way MCB201, Los Angeles, CA 90089 2910, USA
    Yeast 23:173-83. 2006
    ..Here, we present basic methods sufficient for a beginner in this system to carry out most required manipulations for genetic analysis or molecular biology...
  6. ncbi Generating crossovers by resolution of nicked Holliday junctions: a role for Mus81-Eme1 in meiosis
    Fekret Osman
    Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, United Kingdom
    Mol Cell 12:761-74. 2003
    ..These data demonstrate how crossovers could arise without forming or resolving dHJs using an enzyme that is widely conserved amongst eukaryotes...
  7. pmc CENP-A exceeds microtubule attachment sites in centromere clusters of both budding and fission yeast
    Valerie C Coffman
    Department of Molecular Genetics, The Ohio State University, Columbus, OH 43210, USA
    J Cell Biol 195:563-72. 2011
    ..In addition, we show that fission yeast has enough Dam1-DASH complex for ring formation around attached MTs. The results of this study suggest the need for significant revision of existing CEN/kinetochore architectural models...
  8. ncbi Dynamic repertoire of a eukaryotic transcriptome surveyed at single-nucleotide resolution
    Brian T Wilhelm
    Cancer Research UK Fission Yeast Functional Genomics Group, Wellcome Trust Sanger Institute, Hinxton, Cambridge CB10 1HH, UK
    Nature 453:1239-43. 2008
    ..Splicing efficiency was largely coordinated with transcript levels, and increased transcription led to increased splicing in test genes. Hundreds of introns showed such regulated splicing during cellular proliferation or differentiation...
  9. ncbi RNA elimination machinery targeting meiotic mRNAs promotes facultative heterochromatin formation
    Martin Zofall
    Laboratory of Biochemistry and Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Science 335:96-100. 2012
    ..Our analyses uncover unexpected regulatory roles for mRNA-processing factors that assemble dynamic heterochromatin to modulate gene expression...
  10. ncbi Methylation of histone H4 lysine 20 controls recruitment of Crb2 to sites of DNA damage
    Steven L Sanders
    The Wellcome Trust Cancer Research UK Gurdon Institute and Department of Pathology, Tennis Court Road, Cambridge CB2 1QN, United Kingdom
    Cell 119:603-14. 2004
    ..These results argue that H4-K20 methylation functions as a "histone mark" required for the recruitment of the checkpoint protein Crb2...
  11. ncbi A spatial gradient coordinates cell size and mitotic entry in fission yeast
    James B Moseley
    The Rockefeller University, New York, New York 10065, USA
    Nature 459:857-60. 2009
    ..We propose that the Pom1 polar gradient and the medial cortical nodes generate information about cell size and coordinate this with mitotic entry by regulating Cdk1 through Pom1, Cdr2, Cdr1 and Wee1...
  12. ncbi Kinetochore recruitment of two nucleolar proteins is required for homolog segregation in meiosis I
    Kirsten P Rabitsch
    Research Institute of Molecular Pathology, Dr Bohr Gasse 7, A 1030 Vienna, Austria
    Dev Cell 4:535-48. 2003
    ..Csm1 and Pcs1 might clamp together microtubule binding sites on the same (Pcs1) or sister (Csm1) kinetochores...
  13. ncbi The transcriptional program of meiosis and sporulation in fission yeast
    Juan Mata
    The Wellcome Trust Sanger Institute, Cambridge CB10 1SA, UK
    Nat Genet 32:143-7. 2002
    ....
  14. ncbi Meiotic proteins bqt1 and bqt2 tether telomeres to form the bouquet arrangement of chromosomes
    Yuji Chikashige
    Cell Biology Group and CREST Research Project, Kansai Advanced Research Center, National Institute of Information and Communications Technology, 588 2 Iwaoka, Iwaoka cho, Nishi ku, Kobe 651 2492, Japan
    Cell 125:59-69. 2006
    ..Significantly, when both Bqt1 and Bqt2 are ectopically expressed in mitotic cells, they also form a bridge between Rap1 and Sad1. Thus, a complex including Bqt1 and Bqt2 is essential for connecting telomeres to the SPB...
  15. ncbi ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe
    Akihisa Matsuyama
    Chemical Genetics Laboratory, RIKEN, Wako, Saitama 351 0198, Japan
    Nat Biotechnol 24:841-7. 2006
    ..Furthermore, using leptomycin B, an inhibitor of the nuclear export protein Crm1, we identified 285 proteins whose localization is regulated by Crm1...
  16. pmc SUMO-targeted ubiquitin ligases in genome stability
    John Prudden
    Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    EMBO J 26:4089-101. 2007
    ..The DNA repair factor Rad60 and its human homolog NIP45, which contain SLDs, are candidate STUbL targets. Consistently, Rad60 and Slx8-Rfp mutants have similar DNA repair defects...
  17. ncbi Counting cytokinesis proteins globally and locally in fission yeast
    Jian Qiu Wu
    Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06520 8103, USA
    Science 310:310-4. 2005
    ..Proteins concentrated up to 100 times in contractile rings and 7500 times in spindle pole bodies at certain times in the cell cycle. This approach can be used to measure the global and local concentrations of any fusion protein...
  18. ncbi Regulation of heterochromatic silencing and histone H3 lysine-9 methylation by RNAi
    Thomas A Volpe
    Cold Spring Harbor Laboratory, Watson School of Biological Sciences, Cold Spring Harbor, NY 11724, USA
    Science 297:1833-7. 2002
    ..We propose that double-stranded RNA arising from centromeric repeats targets formation and maintenance of heterochromatin through RNAi...
  19. ncbi Gross chromosomal rearrangements and elevated recombination at an inducible site-specific replication fork barrier
    Sarah Lambert
    Genome Damage and Stability Centre, University of Sussex, Brighton BN1 9RQ, UK
    Cell 121:689-702. 2005
    ..Thus, recombination is a "double-edged sword," preventing cell death when the replisome disassembles at the expense of genetic stability...
  20. ncbi Reconstitution of a microtubule plus-end tracking system in vitro
    Peter Bieling
    European Molecular Biology Laboratory, Cell Biology and Biophysics Unit, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    Nature 450:1100-5. 2007
    ..We expect that such in vitro reconstitutions will also be essential for the mechanistic dissection of other plus-end tracking systems...
  21. ncbi Selective recognition of methylated lysine 9 on histone H3 by the HP1 chromo domain
    A J Bannister
    Wellcome CRC Institute and Department of Pathology, University of Cambridge, UK
    Nature 410:120-4. 2001
    ..This model may also explain the stable inheritance of the heterochromatic state...
  22. pmc Oscillatory dynamics of Cdc42 GTPase in the control of polarized growth
    Maitreyi Das
    Department of Molecular and Cellular Pharmacology R 189, University of Miami Miller School of Medicine, Post Office Box 016189, Miami, FL 33101, USA
    Science 337:239-43. 2012
    ..Feedbacks regulating Cdc42 oscillations and spatial self-organization appear to provide a flexible mechanism for fission yeast cells to explore polarization states and to control their morphology...
  23. pmc α-Actinin and fimbrin cooperate with myosin II to organize actomyosin bundles during contractile-ring assembly
    Damien Laporte
    Department of Molecular Genetics, The Ohio State University, Columbus, OH 43210, USA
    Mol Biol Cell 23:3094-110. 2012
    ..Taken together, our work supports a cooperative process of ring self-organization driven by the interaction between actin filaments and myosin II, which is progressively stabilized by the cross-linking proteins...
  24. ncbi The (1,3)beta-D-glucan synthase subunit Bgs1p is responsible for the fission yeast primary septum formation
    Juan Carlos G Cortés
    Instituto de Microbiologia Bioquimica, Consejo Superior de Investigaciones Científicas CSIC Universidad de Salamanca, 37007 Salamanca, Spain
    Mol Microbiol 65:201-17. 2007
    ..We also show that in the absence of Bgs1p abnormal septa are formed, but the cells cannot separate and eventually die...
  25. pmc Nbs1 flexibly tethers Ctp1 and Mre11-Rad50 to coordinate DNA double-strand break processing and repair
    R Scott Williams
    Department of Molecular Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Cell 139:87-99. 2009
    ..Tethering of Ctp1 to a flexible Nbs1 arm suggests a mechanism for restricting DNA end processing and homologous recombination activities of Sae2/Ctp1/CtIP to the immediate vicinity of DSBs...
  26. ncbi Homologous recombination restarts blocked replication forks at the expense of genome rearrangements by template exchange
    Sarah Lambert
    Institut Curie Centre National de la Recherche Scientifique, UMR3348, Réponse Cellulaire aux Perturbations de la Réplication, Universite Paris Sud XI, Orsay, France
    Mol Cell 39:346-59. 2010
    ..In contrast, we report that the Srs2 helicase promotes both fork restart and template exchange. Our data demonstrate that template exchange occurs during recombination-dependent fork restart at the expense of genome rearrangements...
  27. pmc Identification and characterization of two novel proteins affecting fission yeast gamma-tubulin complex function
    Srinivas Venkatram
    Department of Cell and Developmental Biology, Howard Hughes Medical Institute, Vanderbilt University School of Medicine, Nashville, TN 37232, USA
    Mol Biol Cell 15:2287-301. 2004
    ..This study expands the known roles of gamma-tubulin complex components in organizing different types of microtubule structures in S. pombe...
  28. ncbi Selective elimination of messenger RNA prevents an incidence of untimely meiosis
    Yuriko Harigaya
    Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, Hongo, Tokyo 113 0033, Japan
    Nature 442:45-50. 2006
    ..We propose that Mei2 turns off the DSR-Mmi1 system by sequestering Mmi1 to the dot and thereby secures stable expression of meiosis-specific transcripts...
  29. ncbi SHREC, an effector complex for heterochromatic transcriptional silencing
    Tomoyasu Sugiyama
    Laboratory of Molecular Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Cell 128:491-504. 2007
    ..Our analyses suggest that SHREC regulates nucleosome positioning to assemble higher-order chromatin structures critical for heterochromatin functions...
  30. pmc Ctp1 is a cell-cycle-regulated protein that functions with Mre11 complex to control double-strand break repair by homologous recombination
    Oliver Limbo
    Department of Molecular Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Mol Cell 28:134-46. 2007
    ..Transcription of ctp1(+) is periodic during the cell cycle, with the onset of its expression coinciding with the start of DNA replication. These data suggest that regulation of Ctp1 underlies cell-cycle control of HR...
  31. ncbi Polar gradients of the DYRK-family kinase Pom1 couple cell length with the cell cycle
    Sophie G Martin
    Center for Integrative Genomics, Faculty of Biology and Medicine, University of Lausanne, Genopode Building, 1015 Lausanne, Switzerland
    Nature 459:852-6. 2009
    ..In short cells, Pom1 prevents precocious M-phase entry, suggesting that the higher medial Pom1 levels inhibit Cdr2 and promote a G2 delay. Thus, gradients of Pom1 from cell ends provide a measure of cell length to regulate M-phase entry...
  32. pmc The FANCM ortholog Fml1 promotes recombination at stalled replication forks and limits crossing over during DNA double-strand break repair
    Weili Sun
    Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK
    Mol Cell 32:118-28. 2008
    ..In vitro Fml1 catalyzes both replication fork reversal and D loop disruption, indicating possible mechanisms by which it can fulfill its pro- and antirecombinogenic roles...
  33. ncbi Establishment and maintenance of a heterochromatin domain
    Ira M Hall
    Cold Spring Harbor Laboratory, Post Office Box 100, Cold Spring Harbor, NY 11724, USA
    Science 297:2232-7. 2002
    ..This work defines sequential requirements for the initiation and propagation of regional heterochromatic domains...
  34. ncbi Driving the cell cycle with a minimal CDK control network
    Damien Coudreuse
    Laboratory of Yeast Genetics and Cell Biology, The Rockefeller University, 1230 York Avenue, New York, New York 10065, USA
    Nature 468:1074-9. 2010
    ..We propose that this simple core architecture forms the basic control of the eukaryotic cell cycle...
  35. pmc Distinct roles for F-BAR proteins Cdc15p and Bzz1p in actin polymerization at sites of endocytosis in fission yeast
    Rajesh Arasada
    Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, CT 06520 8103, USA
    Curr Biol 21:1450-9. 2011
    ..Many questions remain regarding the interactions of these proteins, particularly the participation of F-BAR proteins in the assembly of actin filaments...
  36. pmc Assembly of the cytokinetic contractile ring from a broad band of nodes in fission yeast
    Jian Qiu Wu
    Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06520, USA
    J Cell Biol 174:391-402. 2006
    ..Their absence delays late steps in cytokinesis, including septum formation and cell separation...
  37. ncbi Contractile-ring assembly in fission yeast cytokinesis: Recent advances and new perspectives
    I Ju Lee
    Department of Molecular Genetics, The Ohio State University, Columbus, Ohio, USA
    Cytoskeleton (Hoboken) 69:751-63. 2012
    ..Collectively, we provide an overview of the current understanding of contractile-ring assembly and uncover future directions in studying cytokinesis in fission yeast...
  38. pmc Conserved function of RNF4 family proteins in eukaryotes: targeting a ubiquitin ligase to SUMOylated proteins
    Huaiyu Sun
    Molecular and Cell Biology Laboratory, Salk Institute for Biological Studies, La Jolla, CA 92037, USA
    EMBO J 26:4102-12. 2007
    ..We identified two related Schizosaccharomyces pombe proteins, Rfp1 and Rfp2, each having an N-terminal SUMO-interacting motif (SIM) and a C-terminal RING-finger ..
  39. pmc Hexanucleotide motifs mediate recruitment of the RNA elimination machinery to silent meiotic genes
    Akira Yamashita
    Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, Hongo, Tokyo 113 0033, Japan
    Open Biol 2:120014. 2012
    ....
  40. ncbi Roles of the fission yeast formin for3p in cell polarity, actin cable formation and symmetric cell division
    B Feierbach
    Department of Microbiology, Columbia University College of Physicians and Surgeons, 701 W 168th Street, New York, NY 10032, USA
    Curr Biol 11:1656-65. 2001
    ..Formins are proteins with conserved roles in cell polarity, cytokinesis, and the regulation of actin and microtubule cytoskeletons...
  41. pmc Dissociation of the Nuf2-Ndc80 complex releases centromeres from the spindle-pole body during meiotic prophase in fission yeast
    Haruhiko Asakawa
    Cell Biology Group and CREST Research Project, Kansai Advanced Research Center, National Institute of Information and Communications Technology, Kobe 651 2492, Japan
    Mol Biol Cell 16:2325-38. 2005
    ....
  42. ncbi The small GTPase Rho3 and the diaphanous/formin For3 function in polarized cell growth in fission yeast
    Kentaro Nakano
    Division of Biology, Department of Life Sciences, Graduate Program of Arts and Sciences, University of Tokyo, Tokyo 153 8902, Japan
    J Cell Sci 115:4629-39. 2002
    ..In conclusion, we propose that the Rho3-For3 signaling system functions in the polarized cell growth of fission yeast by controlling both actin cytoskeleton and MTs...
  43. ncbi Spatial and temporal pathway for assembly and constriction of the contractile ring in fission yeast cytokinesis
    Jian Qiu Wu
    Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06520, USA
    Dev Cell 5:723-34. 2003
    ..Ring contraction and disassembly begin 37 min after SPB separation. This spatial and temporal hierarchy provides the framework for analysis of molecular mechanisms...
  44. ncbi Rho GTPases: regulation of cell polarity and growth in yeasts
    Pilar Perez
    Instituto de Microbiologia Bioquimica, Consejo Superior de Investigaciones Científicas CSIC Universidad de Salamanca, Edificio Departamental, 37007 Salamanca, Spain
    Biochem J 426:243-53. 2010
    ..They provide the co-ordinated regulation of cell-wall biosynthetic enzymes and actin organization required to maintain cell integrity during vegetative growth...
  45. pmc Importance of polyadenylation in the selective elimination of meiotic mRNAs in growing S. pombe cells
    Soichiro Yamanaka
    Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, Hongo, Tokyo, Japan
    EMBO J 29:2173-81. 2010
    ..These patches may represent the sites for degradation of meiosis-specific mRNAs with untimely expression...
  46. ncbi An overview of the fission yeast septation initiation network (SIN)
    Andrea Krapp
    Cell Cycle Control Laboratory, Swiss Institute for Experimental Cancer Research ISREC, School of Life Sciences, Ecole Polytechnique Federale de Lausanne EPFL, 1066 Epalinges s Lausanne, Switzerland
    Biochem Soc Trans 36:411-5. 2008
    ..SIN signalling requires three protein kinases for its function and is mediated by a ras-superfamily GTPase. We discuss the elements of the SIN and how they are regulated...
  47. pmc Understanding cytokinesis: lessons from fission yeast
    Thomas D Pollard
    Thomas D Pollard is at the Department of Molecular Cellular and Developmental Biology, and the Departments of Molecular Biophysics and Biochemistry and of Cell Biology, Yale University, PO Box 208103, New Haven, Connecticut 06520 8103, USA
    Nat Rev Mol Cell Biol 11:149-55. 2010
    ..Many features that have been identified in fission yeast are probably shared with animal cells, as both inherited many cytokinesis genes from their common ancestor about one billion years ago...
  48. ncbi A unified view of the DNA-damage checkpoint
    Justine Melo
    Department of Biochemistry and Biophysics, Mount Zion Cancer Research Center, University of California, San Francisco, CA 94115, USA
    Curr Opin Cell Biol 14:237-45. 2002
    ..This review compares the role of these four classes of checkpoint proteins in humans and model organisms...
  49. ncbi Genome-wide occupancy profile of mediator and the Srb8-11 module reveals interactions with coding regions
    Xuefeng Zhu
    Department Laboratory Medicine, Karolinska Institutet, Novum, SE 141 86 Stockholm, Sweden
    Mol Cell 22:169-78. 2006
    ..We propose that Mediator coordinates transcription initiation with transcriptional events in the coding region of eukaryotic genes...
  50. ncbi Three novel antibiotic marker cassettes for gene disruption and marker switching in Schizosaccharomyces pombe
    Pierre Hentges
    Genome Damage and Stability Centre, University of Sussex, Brighton BN1 9RQ, UK
    Yeast 22:1013-9. 2005
    ..We illustrate the construction of a mutant strain with six independent gene disruptions, using the novel antibiotic cassettes in combination with existing genetic markers...
  51. pmc The fission yeast RNA binding protein Mmi1 regulates meiotic genes by controlling intron specific splicing and polyadenylation coupled RNA turnover
    Huei Mei Chen
    Department of Molecular Genetics and Microbiology, Stony Brook University, Stony Brook, New York, United States of America
    PLoS ONE 6:e26804. 2011
    ..Inactivation of Mmi1 in meiosis allows meiotic expression, through splicing and RNA stabilization, of at least 29 target genes, which are apparently constitutively transcribed...
  52. pmc Topoisomerase I regulates open chromatin and controls gene expression in vivo
    Mickaël Durand-Dubief
    Department of Biosciences and Nutrition, Center for Biosciences, Karolinska Institutet, Novum, Huddinge, Sweden
    EMBO J 29:2126-34. 2010
    ..Our study links topoisomerase activity to the maintenance of open chromatin and regulating transcription in vivo...
  53. pmc Dicer-independent primal RNAs trigger RNAi and heterochromatin formation
    Mario Halic
    Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
    Cell 140:504-16. 2010
    ..Our results suggest that a transcriptome surveillance mechanism based on random association of RNA degradation products with Argonaute triggers siRNA amplification and heterochromatin assembly within DNA repeats...
  54. pmc DNA repair and global sumoylation are regulated by distinct Ubc9 noncovalent complexes
    John Prudden
    Department of Molecular Biology, The Scripps Research Institute, La Jolla, California 92037, USA
    Mol Cell Biol 31:2299-310. 2011
    ..Overall, we determine that, unexpectedly, Ubc9 noncovalent partner choice dictates the role of sumoylation in distinct cellular pathways...
  55. pmc The filament-forming protein Pil1 assembles linear eisosomes in fission yeast
    Ruth Kabeche
    Department of Biochemistry, Dartmouth Medical School, Hanover, NH 03755, USA
    Mol Biol Cell 22:4059-67. 2011
    ..We propose that Pil1 is a novel component of the yeast cytoskeleton, with implications for the role of filament assembly in the spatial organization of cells...
  56. pmc Roles of formin nodes and myosin motor activity in Mid1p-dependent contractile-ring assembly during fission yeast cytokinesis
    Valerie C Coffman
    Department of Molecular Genetics, Graduate Program of Molecular, Cellular, and Developmental Biology, The Ohio State University, Columbus, OH 43210, USA
    Mol Biol Cell 20:5195-210. 2009
    ..Taken together, these data provide strong support for the SCPR model of contractile-ring formation in cytokinesis...
  57. pmc The kinetochore proteins Pcs1 and Mde4 and heterochromatin are required to prevent merotelic orientation
    Juraj Gregan
    Max F Perutz Laboratories, Department of Chromosome Biology, University of Vienna, Dr Bohr Gasse 1, 1030 Vienna, Austria
    Curr Biol 17:1190-200. 2007
    ..A protein called Pcs1 has been implicated in preventing merotelic attachment in mitosis and meiosis II in the fission yeast S. pombe...
  58. ncbi Heterochromatin revisited
    Shiv I S Grewal
    Laboratory of Molecular Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Nat Rev Genet 8:35-46. 2007
    ....
  59. pmc The Rho1p exchange factor Rgf1p signals upstream from the Pmk1 mitogen-activated protein kinase pathway in fission yeast
    Patricia Garcia
    Instituto de Microbiologia Bioquimica, CSIC Universidad de Salamanca and Departamento de Microbiología y Genética, Universidad de Salamanca, Campus Miguel de Unamuno, 37007 Salamanca, Spain
    Mol Biol Cell 20:721-31. 2009
    ....
  60. pmc Role of the Small GTPase Rho3 in Golgi/Endosome trafficking through functional interaction with adaptin in Fission Yeast
    Ayako Kita
    Laboratory of Molecular Pharmacogenomics, School of Pharmaceutical Sciences, Kinki University, Higashi Osaka, Japan
    PLoS ONE 6:e16842. 2011
    ....
  61. pmc Anillin-related protein Mid1p coordinates the assembly of the cytokinetic contractile ring in fission yeast
    Shambaditya Saha
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520 8103, USA
    Mol Biol Cell 23:3982-92. 2012
    ..We conclude that orderly assembly of the contractile ring in wild-type cells depends on Mid1p to recruit myosin-II, Rng2p, and Cdc15p to nodes and to place cytokinetic nodes around the cell equator...
  62. pmc Characterization of Mid1 domains for targeting and scaffolding in fission yeast cytokinesis
    I Ju Lee
    Department of Molecular Genetics, The Ohio State University, Columbus, OH 43210, USA
    J Cell Sci 125:2973-85. 2012
    ..Collectively, our study recognizes domains regulating Mid1 cortical localization and reveals domains sufficient for contractile-ring assembly...
  63. ncbi Role of histone H3 lysine 9 methylation in epigenetic control of heterochromatin assembly
    J Nakayama
    Cold Spring Harbor Laboratory, Post Office Box 100, Cold Spring Harbor, NY 11724, USA
    Science 292:110-3. 2001
    ..These data define a conserved pathway wherein sequential histone modifications establish a "histone code" essential for the epigenetic inheritance of heterochromatin assembly...
  64. pmc A mutation in gamma-tubulin alters microtubule dynamics and organization and is synthetically lethal with the kinesin-like protein pkl1p
    J L Paluh
    Department of Molecular Biology, University of California, Berkeley, California 94720 3200, USA
    Mol Biol Cell 11:1225-39. 2000
    ..We propose that Pkl1p rescues an impaired function of gamma-tubulin that involves non-tubulin protein-protein interactions, presumably with a second motor, MAP, or MTOC component...
  65. ncbi Wsh3/Tea4 is a novel cell-end factor essential for bipolar distribution of Tea1 and protects cell polarity under environmental stress in S. pombe
    Hisashi Tatebe
    Section of Microbiology, Division of Biological Sciences, University of California, Davis, Davis, California 95616, USA
    Curr Biol 15:1006-15. 2005
    ..The deltatea1 mutant cells cannot grow in a bipolar fashion and show T-shaped morphology after heat shock...
  66. pmc Autoregulated expression of Schizosaccharomyces pombe meiosis-specific transcription factor Mei4 and a genome-wide search for its target genes
    H Abe
    Department of Biology, Graduate School of Science, Osaka City University, Sumiyoshi ku, Osaka 558 8585, Japan
    Genetics 154:1497-508. 2000
    ..Furthermore, expression of a reporter gene driven by the authentic mei4 promoter was induced in vegetative cells by ectopic overproduction of Mei4p. These results suggest that mei4 transcription is positively autoregulated...
  67. ncbi Cdc42 regulates multiple membrane traffic events in fission yeast
    Miguel Estravís
    Instituto de Biología Funcional y Genómica, Consejo Superior de Investigaciones Científicas CSIC Departamento de Microbiología y Genética, Universidad de Salamanca, 30007 Salamanca, Spain
    Traffic 12:1744-58. 2011
    ..All together, we propose that Cdc42 and the scaffold Pob1 are required for membrane trafficking and fusion, contributing to polarized secretion, endosome recycling, vacuole formation and growth...
  68. ncbi Heterochromatin integrity affects chromosome reorganization after centromere dysfunction
    Kojiro Ishii
    Division of Cell Biology, Institute of Life Science, Kurume University, Japan
    Science 321:1088-91. 2008
    ..By affecting the modes of chromosomal reorganization, the genomic distribution of heterochromatin may influence the fate of karyotype evolution...
  69. pmc Nucleosomal organization of replication origins and meiotic recombination hotspots in fission yeast
    Elisa de Castro
    Instituto de Biología Funcional y Genómica, CSIC Universidad de Salamanca, Edificio Departamental, Campus Miguel de Unamuno, Salamanca, Spain
    EMBO J 31:124-37. 2012
    ..The organization of ORIs and of DSBs regions in S. pombe reveals similarities and differences relative to Saccharomyces cerevisiae...
  70. pmc The fission yeast stress-responsive MAPK pathway promotes meiosis via the phosphorylation of Pol II CTD in response to environmental and feedback cues
    Yuko Sukegawa
    Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, Tokyo, Japan
    PLoS Genet 7:e1002387. 2011
    ..This study also demonstrates clearly that the stress-responsive MAP kinase pathway can modulates gene expression through phosphorylation of Pol II CTD...
  71. ncbi Role of actin polymerization and actin cables in actin-patch movement in Schizosaccharomyces pombe
    R J Pelham
    Department of Microbiology, Columbia University College of Physicians and Surgeons, 701 West 168th Street, New York, New York 10032, USA
    Nat Cell Biol 3:235-44. 2001
    ..Our studies give rise to a model in which actin patches are centres for actin polymerization that drive their own movement on actin cables using Arp2/3-based actin polymerization...
  72. pmc Casein kinase 1 is required for efficient removal of Rec8 during meiosis I
    Cornelia Rumpf
    Max F Perutz Laboratories, University of Vienna, Vienna, Austria
    Cell Cycle 9:2657-62. 2010
    ..Our data are consistent with the model that Hhp1/Hhp2-dependent phosphorylation of Rec8 is required for separase-mediated cleavage of Rec8 during meiosis I...
  73. pmc DNA replication through hard-to-replicate sites, including both highly transcribed RNA Pol II and Pol III genes, requires the S. pombe Pfh1 helicase
    Nasim Sabouri
    Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544, USA
    Genes Dev 26:581-93. 2012
    ..Thus, Pfh1 promotes DNA replication and separation of converged replication forks and suppresses DNA damage at hard-to-replicate sites...
  74. pmc Telomere-led bouquet formation facilitates homologous chromosome pairing and restricts ectopic interaction in fission yeast meiosis
    O Niwa
    Kazusa DNA Research Institute, 1532 3 Yana, Kisarazu, Chiba 292 0812, Japan
    EMBO J 19:3831-40. 2000
    ..Thus, telomere-led chromosome organization facilitates homologous pairing and also restricts irregular chromosome pairing during meiosis...
  75. pmc TRAMP-mediated RNA surveillance prevents spurious entry of RNAs into the Schizosaccharomyces pombe siRNA pathway
    Marc Buhler
    Department of Cell Biology, 240 Longwood Avenue, Harvard Medical School, Boston, Massachusetts 02115 USA
    Nat Struct Mol Biol 15:1015-23. 2008
    ..Thus, Cid14 prevents certain abundant RNAs from becoming substrates for the RNAi machinery, thereby freeing the RNAi machinery to act on its proper targets...
  76. pmc Fission yeast Mus81.Eme1 Holliday junction resolvase is required for meiotic crossing over but not for gene conversion
    Gerald R Smith
    Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA
    Genetics 165:2289-93. 2003
    ..Thus, gene conversion and crossing over can be genetically separated, and Mus81 is required for crossing over, supporting the hypothesis that the fission yeast Mus81.Eme1 protein complex resolves Holliday junctions in meiotic cells...
  77. pmc Decapping is preceded by 3' uridylation in a novel pathway of bulk mRNA turnover
    Olivia S Rissland
    Sir William Dunn School of Pathology, University of Oxford, UK
    Nat Struct Mol Biol 16:616-23. 2009
    ..As human cells contain Cid1 orthologs, uridylation may form the basis of a widespread, conserved mechanism of mRNA decay...
  78. pmc Effects of {gamma}-tubulin complex proteins on microtubule nucleation and catastrophe in fission yeast
    Sabina Zimmerman
    Department of Microbiology, Columbia University College of Physicians and Surgeons, New York, NY 10032, USA
    Mol Biol Cell 16:2719-33. 2005
    ..Our findings show that mto1p and gamma-TuCs affect multiple properties of MTs including nucleation, nuclear attachment, plus-end catastrophe, and minus-end shrinkage...
  79. pmc The Schizosaccharomyces pombe mei4+ gene encodes a meiosis-specific transcription factor containing a forkhead DNA-binding domain
    S Horie
    Department of Biology, Faculty of Science, Osaka City University, Osaka, Japan
    Mol Cell Biol 18:2118-29. 1998
    ..These results indicate that Mei4 functions as a meiosis-specific transcription factor of S. pombe...
  80. pmc Ndc80 internal loop interacts with Dis1/TOG to ensure proper kinetochore-spindle attachment in fission yeast
    Kuo Shun Hsu
    Laboratory of Cell Regulation, Cancer Research UK, London Research Institute, Lincoln s Inn Fields Laboratories, 44 Lincoln s Inn Fields, London WC2A 3LY, UK
    Curr Biol 21:214-20. 2011
    ..Therefore, our results provide a crucial insight into how the Ndc80 complex establishes stable bipolar attachment to the spindle microtubule...
  81. ncbi swi1 and swi3 perform imprinting, pausing, and termination of DNA replication in S. pombe
    J Z Dalgaard
    DHHS, NCI, Division of Basic Sciences Gene Regulation and Chromosome Biology Laboratory, NCI Frederick Cancer Research and Development Center, Maryland 21702, USA
    Cell 102:745-51. 2000
    ..These results suggest that swi1p and swi3p promote imprinting in novel ways both by pausing replication at mat1 and by terminating replication at RTS1...
  82. pmc Loss of Apm1, the micro1 subunit of the clathrin-associated adaptor-protein-1 complex, causes distinct phenotypes and synthetic lethality with calcineurin deletion in fission yeast
    Ayako Kita
    Division of Molecular Pharmacology and Pharmacogenomics, Department of Genome Sciences, Kobe University Graduate School of Medicine, Kobe 650 0017, Japan
    Mol Biol Cell 15:2920-31. 2004
    ....
  83. pmc Spindle checkpoint silencing requires association of PP1 to both Spc7 and kinesin-8 motors
    John C Meadows
    Division of Biomedical Cell Biology, Warwick Medical School, University of Warwick, Gibbet Hill, Coventry, UK
    Dev Cell 20:739-50. 2011
    ..These data demonstrate that at least two distinct pools of PP1, one kinetochore associated and the other motor associated, are needed to silence the spindle checkpoint...
  84. ncbi Two-hybrid search for proteins that interact with Sad1 and Kms1, two membrane-bound components of the spindle pole body in fission yeast
    F Miki
    Kazusa DNA Research Institute, 292 0818, Kisarazu, Chiba, Japan
    Mol Genet Genomics 270:449-61. 2004
    ..We found that none of the newly identified genes tested were necessary for centromere/telomere clustering...
  85. pmc A Caenorhabditis elegans cohesion protein with functions in meiotic chromosome pairing and disjunction
    P Pasierbek
    Department of Cytology and Genetics, Institute of Botany, University of Vienna, A 1030 Vienna, Austria
    Genes Dev 15:1349-60. 2001
    ..The loss of yeast Rec8p from chromosome arms at meiosis I and centromeres at meiosis II coordinates the disjunction of homologs and sister chromatids at the two meiotic divisions...
  86. pmc Transcriptional regulatory network for sexual differentiation in fission yeast
    Juan Mata
    Cancer Research UK Fission Yeast Functional Genomics Group, Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge CB10 1HH, UK
    Genome Biol 8:R217. 2007
    ..The expression of hundreds of genes is modulated in successive waves during meiosis and sporulation in S. pombe, and several known transcription factors are critical for these processes...
  87. pmc RNA Pol II subunit Rpb7 promotes centromeric transcription and RNAi-directed chromatin silencing
    Ingela Djupedal
    Karolinska Institutet, Department of Biosciences, School of Life Sciences, University College Sodertorn, S 141 89 Huddinge, Sweden
    Genes Dev 19:2301-6. 2005
    ..In contrast to other Pol II subunits, Rpb7 promotes pre-siRNA transcription required for RNAi-directed chromatin silencing...
  88. pmc Fission yeast Scm3: A CENP-A receptor required for integrity of subkinetochore chromatin
    Alison L Pidoux
    Wellcome Trust Centre for Cell Biology and Institute of Cell Biology, School of Biological Sciences, The University of Edinburgh, 6 34 Swann Building, Edinburgh EH93JR, Scotland, UK
    Mol Cell 33:299-311. 2009
    ....
  89. ncbi Deletion mutants of AP-1 adaptin subunits display distinct phenotypes in fission yeast
    Yan Ma
    Division of Molecular Pharmacology and Pharmacogenomics, Department of Biochemistry and Molecular Biology, Kobe University Graduate School of Medicine, Kusunoki cho, Chuo Ku, Kobe 650 0017, Japan
    Genes Cells 14:1015-28. 2009
    ..Altogether, results suggest that the four subunits are all essential for the heterotetrameric complex formation and for the AP-1 function in exit transport from endosomes...
  90. ncbi Clr4/Suv39 and RNA quality control factors cooperate to trigger RNAi and suppress antisense RNA
    Ke Zhang
    Laboratory of Biochemistry and Molecular Biology, National Cancer Institute NIH, Bethesda, MD 20892, USA
    Science 331:1624-7. 2011
    ..These findings link Clr4 to RNA quality control machinery and suggest a pathway for processing potentially deleterious RNAs through the coordinated actions of RNAi and other RNA processing activities...
  91. pmc Fission yeast pap1-dependent transcription is negatively regulated by an essential nuclear protein, crm1
    T Toda
    Department of Biophysics, Faculty of Science, Kyoto University, Japan
    Mol Cell Biol 12:5474-84. 1992
    ..These results suggest the functional importance of chromosome structure on the regulation of gene expression through the pap1 transcription factor...
  92. pmc Mid1p/anillin and the septation initiation network orchestrate contractile ring assembly for cytokinesis
    Olivier Hachet
    Cell Cycle Control Laboratory, Swiss Institute for Experimental Cancer Research ISREC, School of Life Sciences, Ecole Polytechnique Federale de Lausanne EPFL, 1066 Epalinges s Lausanne, Switzerland
    Genes Dev 22:3205-16. 2008
    ..Taken together, these data establish that Mid1p and the SIN are the key regulators that orchestrate CAR assembly...
  93. pmc The nuclear kinase Lsk1p positively regulates the septation initiation network and promotes the successful completion of cytokinesis in response to perturbation of the actomyosin ring in Schizosaccharomyces pombe
    Jim Karagiannis
    Laboratory of Cell Division, Temasek Life Sciences Laboratory, Singapore 117604, Singapore
    Mol Biol Cell 16:358-71. 2005
    ..Our studies also establish that actomyosin ring maintenance and nuclear cycle delay in response to cytokinetic perturbation can be genetically resolved into independent pathways...
  94. pmc The SH3 domains of two PCH family members cooperate in assembly of the Schizosaccharomyces pombe contractile ring
    Rachel H Roberts-Galbraith
    Howard Hughes Medical Institute, Chevy Chase, MD 20815, USA
    J Cell Biol 184:113-27. 2009
    ..Our data indicate that the F-BAR proteins Cdc15 and Imp2 contribute to a single biological process with both distinct and overlapping functions...
  95. pmc swi1- and swi3-dependent and independent replication fork arrest at the ribosomal DNA of Schizosaccharomyces pombe
    Gregor Krings
    Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston, SC 29425, USA
    Proc Natl Acad Sci U S A 101:14085-90. 2004
    ..The results support the notion that Swi1p and Swi3p act at several different protein-DNA complexes in the rDNA spacer regions to arrest replication but that not all fork barriers required their activity to arrest forks...
  96. ncbi A phosphorylation cycle shapes gradients of the DYRK family kinase Pom1 at the plasma membrane
    Olivier Hachet
    Department of Fundamental Microbiology, Faculty of Biology and Medicine, University of Lausanne, CH 1015 Lausanne, Switzerland
    Cell 145:1116-28. 2011
    ..We propose that local dephosphorylation induces Pom1 membrane association and nucleates a gradient shaped by the opposing actions of lateral diffusion and autophosphorylation-dependent membrane detachment...
  97. pmc The major role of human AP-endonuclease homolog Apn2 in repair of abasic sites in Schizosaccharomyces pombe
    Balazs Ribar
    Sealy Center for Molecular Science, University of Texas Medical Branch, 6 148 Medical Research Building, Galveston, TX 77555, USA
    Nucleic Acids Res 32:115-26. 2004
    ..We confirmed the presence of APE activity in recombinant Apn2 and in crude cell extracts. Thus S.pombe is distinct from S.cerevisiae, and is similar to mammalian cells in having Apn2 as the major APE...
  98. pmc RNase-sensitive DNA modification(s) initiates S. pombe mating-type switching
    Sonya Vengrova
    Marie Curie Research Institute, The Chart, Oxted, Surrey RH8 0TL, UK
    Genes Dev 18:794-804. 2004
    ..We propose a model in which a swi1- and swi3-dependent signal during lagging-strand synthesis leads to pausing of leading-strand replication and the introduction of the imprint...
  99. pmc A supramodular FHA/BRCT-repeat architecture mediates Nbs1 adaptor function in response to DNA damage
    Janette Lloyd
    Division of Molecular Structure, Medical Research Council National Institute for Medical Research, The Ridgeway, Mill Hill, London, UK
    Cell 139:100-11. 2009
    ..Finally, we establish that human Nbs1 interactions with Mdc1 occur through both its FHA- and BRCT-repeat domains, suggesting how their structural and functional interdependence underpins Nbs1 adaptor functions in the DNA-damage response...
  100. ncbi Schizosaccharomyces pombe ste11+ encodes a transcription factor with an HMG motif that is a critical regulator of sexual development
    A Sugimoto
    Department of Biophysics and Biochemistry, Faculty of Science, University of Tokyo, Japan
    Genes Dev 5:1990-9. 1991
    ..These observations strongly suggest that Ste11 serves as a key transcription factor for sexual development...
  101. ncbi The nuclear poly(A)-binding protein interacts with the exosome to promote synthesis of noncoding small nucleolar RNAs
    Jean François Lemay
    RNA Group, Department of Biochemistry, Universite de Sherbrooke, Sherbrooke, QC JIH 5N4, Canada
    Mol Cell 37:34-45. 2010
    ..This work unveils a function for the nuclear PABP in snoRNA synthesis and provides insights into exosome recruitment to polyadenylated RNAs...

Research Grants44

  1. Conference on Wiring and Plasticity of Nervous Systems
    Thomas Pollard; Fiscal Year: 2002
    ..We are confident that mutually beneficial insights will emerge from learning about each others work. ..
  2. Experimental and Computational Studies of Exit from Mitosis in Budding Yeast
    John J Tyson; Fiscal Year: 2010
    ..g., embryonic development, tissue regeneration, wound healing, and carcinogenesis. ..
  3. 2003 Genetic Toxicology Gordon Conference
    Anthony Carr; Fiscal Year: 2007
    ..We particularly air to obtain funding to support applications from minority groupings and younger scientists. We have already identified a significant participation of women amongst our list of speakers. ..
  4. Problem Solving Environment for Modeling the Cell Cycle
    John Tyson; Fiscal Year: 2002
    ..and some outstanding collaborators (Sible and Cross, experimental cell cycle research; Kitano and Bolouri, software development for molecular cell biology; and Kohn, bioinformatics of mammalian cell cycle controls). ..
  5. Signal Amplification by an RTK/Abl Kinase Module
    Ann Marie Pendergast; Fiscal Year: 2007
    ..unreadable] [unreadable]..
  6. Structural Analyses of Fission Yeast Splicing Complexes
    KATHLEEN GOULD; Fiscal Year: 2006
    ..abstract_text> ..
  7. Molecular Analysis of the Kinetochore
    SONI SHIMODA; Fiscal Year: 2006
    ..A characterization of the proteins involved in chromosome segregation and checkpoint activation could lead to a greater understanding of how human diseases, such as cancer, can be prevented. [unreadable] [unreadable]..
  8. Structural Basis of Membrane Targeting by NADPH Oxidase
    David A Horita; Fiscal Year: 2010
    ..These experiments will provide key insights into how oxidase activity is regulated and the differences in regulation and activation of phagocytic vs. nonphagocytic forms of the oxidase. ..
  9. Regulated cytoskeletal dynamics in the secretory pathway
    Mark Stamnes; Fiscal Year: 2006
    ..abstract_text> ..
  10. MOLECULAR BASIS OF SIGNALING BY THE CABL PROTO-ONCOGENE
    Ann Marie Pendergast; Fiscal Year: 2010
    ..abstract_text> ..
  11. Telomerase function and regulation in a new model system
    Neal Lue; Fiscal Year: 2007
    ..We anticipate that our findings will contribute to a detailed molecular understanding of telomerase function and regulation. ..
  12. Amygdala, CREB, and Neuropathic Pain Facilitation
    JENNIFER PETTA; Fiscal Year: 2007
    ..Thus, the experiments in the current proposal investigating the role of the amygdala in chronic pain are timely and may lead to more effective drug targets. [unreadable] [unreadable] [unreadable]..
  13. Sequence Determinants of Meiotic Recombination Hotspots
    Walter Steiner; Fiscal Year: 2007
    ..pombe are also active in the distantly related budding yeast Saccharomyces cerevisiae. Sequences that are active in both organisms may be widely conserved among eukaryotes, including man. [unreadable] [unreadable] [unreadable]..
  14. Human Hematopoietic Cell Transformation by BCR/ABL
    Ravi Bhatia; Fiscal Year: 2008
    ..These studies are expected to result in improved understanding of mechanisms critical for human progenitor transformation in CML and guide rational development of additional mechanism-based therapies. ..
  15. MOLECULAR ANALYSIS OF HOTSPOTS OF GENETIC RECOMBINATION
    Gerald Smith; Fiscal Year: 2007
    ..Thus, the basic research proposed here will add to the foundations for understanding, diagnosing, preventing, and curing human disease. ..
  16. Abnormalities in gene expression in therapy-related MDS
    Ravi Bhatia; Fiscal Year: 2008
    ..These studies may also allow identification of markers of susceptibility to t-MDS allowing early detection of patients at risk for this complication. ..
  17. Mouse models for GABA epigenetic dysfunction
    Alessandro Guidotti; Fiscal Year: 2009
    ..abstract_text> ..
  18. Intercellular adhesion and morphogenesis: role of actin-regulatory proteins
    Ann Marie Pendergast; Fiscal Year: 2010
    ..Moreover, our findings will provide much needed knowledge for elucidating the molecular basis of develomental abnormalities such as congenital cardiovascular disease and other pathologies induced by disregulated actin dynamics. ..
  19. ANALYSIS OF TELOMERASE REVERSE TRANSCRIPTASE
    Neal F Lue; Fiscal Year: 2010
    ....
  20. BIOGENESIS OF THE YEAST MITOCHONDRIAL ATPASE COMPLEX
    Sharon Ackerman; Fiscal Year: 2006
    ....
  21. Structure based drug discovery
    STEPHEN BURLEY; Fiscal Year: 2006
    ..unreadable] [unreadable]..
  22. Structural Genomics
    STEPHEN BURLEY; Fiscal Year: 2004
    ..Finally, this meeting is being held in conjunction with the 2004 Keystone Structural Biology Meeting, and features three joint sessions (including the Keynote). ..
  23. Gene Activation by Remote Transcriptional Enhancers
    Dale Dorsett; Fiscal Year: 2004
    ..This will help illuminate the basic mechanisms underlying gene expression defects that occur in some human diseases. ..
  24. NEGATIVE REGULATION OF CELL CYCLE PROGRESSION
    M Hoyt; Fiscal Year: 2004
    ..The identified genes will report checkpoint activation and will allow a powerful dissection of this signal transduction pathway. ..
  25. BCR/ABL SIGNALING IN HUMAN LEUKEMIAS
    Ann Marie Pendergast; Fiscal Year: 2003
    ..Definition of this pathway may allow for the development of therapeutic reagents for the treatment of leukemias and other cancers that are caused by the activation of nonreceptor tyrosine kinases. ..
  26. Structural Biology of Tyrosine Kinase Regulation
    David Horita; Fiscal Year: 2004
    ..These studies will establish the feasibility of comprehensive analysis of tyrosine kinase structure and dynamics using NMR. ..
  27. REGULATION OF MITOSIS IN FISSION YEAST
    Paul Russell; Fiscal Year: 2003
    ..The aim of these experiments will be to understand how activation of nuclear Cdc2/cyclin-B is coordinated with signals that indicate cell size and cell physiology that are presumed to emanate from cytoplasm. ..
  28. REELIN DEFICIENCY--MODEL FOR SCHIZOPHRENIA VULNERABILITY
    Alessandro Guidotti; Fiscal Year: 2004
    ....
  29. X-RAY STRUCTURES OF EUKARYOTIC TRANSLATION FACTORS
    STEPHEN BURLEY; Fiscal Year: 2003
    ..Specific Aim 6. Determine X-ray structures of PABP recognizing poly (A) RNA, and various binary and ternary complexes with a PABP-interacting protein and eIF4G. ..
  30. POSTTRANSLATIONAL REGULATION OF HSF ACTIVITY
    James Bonner; Fiscal Year: 2002
    ..These will identify those cellular systems that require HSF activity in the absence of stress, and thus reveal why HSF is an essential gene in yeast. ..
  31. PHARMACOLOGY--ALLOSTERIC MODULATORS OF GABAA RECEPTORS
    Alessandro Guidotti; Fiscal Year: 2002
    ....
  32. MECHANISMS OF EUKARYOTIC CELL CYCLE CONTROL
    KATHLEEN GOULD; Fiscal Year: 2004
    ..Our long-term goal is to understand how the pathways in which cdc5p and dim1p function are coordinated with known elements regulating entry into mitosis. ..
  33. Defining the Role of Mcm10 in DNA Replication
    Ming Lei; Fiscal Year: 2005
    ..These studies will provide a unique perspective to the understanding of origin activity and the interactions between the origin and the replication fork. ..
  34. Regulation of DNA replication and the S phase checkpoint
    PEI YUN WU; Fiscal Year: 2007
    ..The experiments in this proposal are all important for understanding the onset of cancer and its treatment. [unreadable] [unreadable]..
  35. TYROSINE KINASES AND ADAPTOR PROTEINS IN CELL MIGRATION
    Ann Marie Pendergast; Fiscal Year: 2005
    ..abstract_text> ..
  36. Structural investigation of Arp2/3 complex activation
    Bradley Nolen; Fiscal Year: 2006
    ..Furthermore, it will show how nucleation promoting factors activate the complex. This will provide a basis for a better understanding of cytoskeletal function in both normal and diseased states of the cell. ..
  37. REGULATION OF THE ONSET OF ANAPHASE
    Tomohiro Matsumoto; Fiscal Year: 2001
    ....
  38. Structure Determination of the Polymerase alpha-primase
    DEWIGHT WILLIAMS; Fiscal Year: 2006
    ..abstract_text> ..
  39. Assembling a Centric Nucleosome
    Richard Baker; Fiscal Year: 2004
    ..Given the high conservation of centric histone H3 variants throughout evolution, the results will illuminate the problem of centromere formation and function in higher eukaryotes including humans. ..
  40. Regulation and function of the Clp 1p protein phosphatase
    Dannel McCollum; Fiscal Year: 2010
    ..3) To test a hypothesis that Clp1 promotes actomyosin ring stability through effects on Cdc15. 4) To determine how Clp1 and the anaphase promoting complex promote Cdc25 inactivation in late mitosis. ..
  41. Regulation and Function of MAPKAP Kinases
    Thomas Sturgill; Fiscal Year: 2005
    ..Aim four is to characterize the activation, enzymatic properties, and functions of MAPKAP kinase-like enzymes (Rcklp, Rck2p) in yeast, a model eukaryote, using combined biochemical and genetic approaches. ..
  42. mTOR Signaling Pathways
    Thomas W Sturgill; Fiscal Year: 2010
    ..We will investigate novel candidate mTOR-interacting proteins identified by MS. Finally, to understand better the mechanisms involved in mTORC2, we propose to identify pianissimo-interacting proteins. ..
  43. Nuclear envelope membrane proteins and nuclear structure
    Megan King; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..