telomere binding proteins

Summary

Summary: Proteins that specifically bind to TELOMERES. Proteins in this class include those that perform functions such as telomere capping, telomere maintenance and telomere stabilization.

Top Publications

  1. ncbi Shelterin: the protein complex that shapes and safeguards human telomeres
    Titia de Lange
    The Rockefeller University, New York, New York 10021, USA
    Genes Dev 19:2100-10. 2005
  2. ncbi Meiotic proteins bqt1 and bqt2 tether telomeres to form the bouquet arrangement of chromosomes
    Yuji Chikashige
    Cell Biology Group and CREST Research Project, Kansai Advanced Research Center, National Institute of Information and Communications Technology, 588 2 Iwaoka, Iwaoka cho, Nishi ku, Kobe 651 2492, Japan
    Cell 125:59-69. 2006
  3. ncbi TPP1 is a homologue of ciliate TEBP-beta and interacts with POT1 to recruit telomerase
    Huawei Xin
    Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, One Baylor Plaza, Houston, Texas 77030, USA
    Nature 445:559-62. 2007
  4. ncbi POT1 as a terminal transducer of TRF1 telomere length control
    Diego Loayza
    Laboratory for Cell Biology and Genetics, The Rockefeller University, 1230 York Avenue, New York, New York 10021, USA
    Nature 423:1013-8. 2003
  5. ncbi A RAP1-interacting protein involved in transcriptional silencing and telomere length regulation
    C F Hardy
    Department of Microbiology, College of Physicians and Surgeons, Columbia University, New York, New York 10032
    Genes Dev 6:801-14. 1992
  6. ncbi TIN2, a new regulator of telomere length in human cells
    S H Kim
    Department of Cell and Molecular Biology, Lawrence Berkeley National Laboratory, Berkeley, California, USA
    Nat Genet 23:405-12. 1999
  7. ncbi Structure of the TRFH dimerization domain of the human telomeric proteins TRF1 and TRF2
    L Fairall
    MRC Laboratory of Molecular Biology, Hills Road, CB2 2QH, Cambridge, United Kingdom
    Mol Cell 8:351-61. 2001
  8. pmc Human Pot1 (protection of telomeres) protein: cytolocalization, gene structure, and alternative splicing
    Peter Baumann
    Howard Hughes Medical Institute, Department of Chemistry and Biochemistry, University of Colorado, Boulder, Colorado 80309 0215, USA
    Mol Cell Biol 22:8079-87. 2002
  9. ncbi A genome-wide screen identifies the evolutionarily conserved KEOPS complex as a telomere regulator
    Michael Downey
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, 600 University Avenue, Toronto, ON, M5G 1X5, Canada
    Cell 124:1155-68. 2006
  10. pmc Three Ever Shorter Telomere (EST) genes are dispensable for in vitro yeast telomerase activity
    J Lingner
    Howard Hughes Medical Institute, Department of Chemistry and Biochemistry, University of Colorado, Boulder, CO 80309 0215, USA
    Proc Natl Acad Sci U S A 94:11190-5. 1997

Detail Information

Publications228 found, 100 shown here

  1. ncbi Shelterin: the protein complex that shapes and safeguards human telomeres
    Titia de Lange
    The Rockefeller University, New York, New York 10021, USA
    Genes Dev 19:2100-10. 2005
    ..Six shelterin subunits: TRF1, TRF2, TIN2, Rap1, TPP1, and POT1...
  2. ncbi Meiotic proteins bqt1 and bqt2 tether telomeres to form the bouquet arrangement of chromosomes
    Yuji Chikashige
    Cell Biology Group and CREST Research Project, Kansai Advanced Research Center, National Institute of Information and Communications Technology, 588 2 Iwaoka, Iwaoka cho, Nishi ku, Kobe 651 2492, Japan
    Cell 125:59-69. 2006
    ..Significantly, when both Bqt1 and Bqt2 are ectopically expressed in mitotic cells, they also form a bridge between Rap1 and Sad1. Thus, a complex including Bqt1 and Bqt2 is essential for connecting telomeres to the SPB...
  3. ncbi TPP1 is a homologue of ciliate TEBP-beta and interacts with POT1 to recruit telomerase
    Huawei Xin
    Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, One Baylor Plaza, Houston, Texas 77030, USA
    Nature 445:559-62. 2007
    ....
  4. ncbi POT1 as a terminal transducer of TRF1 telomere length control
    Diego Loayza
    Laboratory for Cell Biology and Genetics, The Rockefeller University, 1230 York Avenue, New York, New York 10021, USA
    Nature 423:1013-8. 2003
    ....
  5. ncbi A RAP1-interacting protein involved in transcriptional silencing and telomere length regulation
    C F Hardy
    Department of Microbiology, College of Physicians and Surgeons, Columbia University, New York, New York 10032
    Genes Dev 6:801-14. 1992
    ..By use of the two-hybrid system, we have isolated a mutation in RIF1 that partially restores the interaction with rap1s mutant proteins...
  6. ncbi TIN2, a new regulator of telomere length in human cells
    S H Kim
    Department of Cell and Molecular Biology, Lawrence Berkeley National Laboratory, Berkeley, California, USA
    Nat Genet 23:405-12. 1999
    ..Our findings suggest that TRF1 is insufficient for control of telomere length in human cells, and that TIN2 is an essential mediator of TRF1 function...
  7. ncbi Structure of the TRFH dimerization domain of the human telomeric proteins TRF1 and TRF2
    L Fairall
    MRC Laboratory of Molecular Biology, Hills Road, CB2 2QH, Cambridge, United Kingdom
    Mol Cell 8:351-61. 2001
    ..A possible structural homology between the TRFH domain of fission yeast telomeric protein Taz1 with those of the vertebrate TRFs is suggested...
  8. pmc Human Pot1 (protection of telomeres) protein: cytolocalization, gene structure, and alternative splicing
    Peter Baumann
    Howard Hughes Medical Institute, Department of Chemistry and Biochemistry, University of Colorado, Boulder, Colorado 80309 0215, USA
    Mol Cell Biol 22:8079-87. 2002
    ..In addition to these splice variants, the Pot1 family is expanded by the identification of six more genes from diverse species. Pot1-like proteins have now been found in plants, animals, yeasts, and microsporidia...
  9. ncbi A genome-wide screen identifies the evolutionarily conserved KEOPS complex as a telomere regulator
    Michael Downey
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, 600 University Avenue, Toronto, ON, M5G 1X5, Canada
    Cell 124:1155-68. 2006
    ..Our results therefore indicate that the KEOPS complex promotes both telomere uncapping and telomere elongation...
  10. pmc Three Ever Shorter Telomere (EST) genes are dispensable for in vitro yeast telomerase activity
    J Lingner
    Howard Hughes Medical Institute, Department of Chemistry and Biochemistry, University of Colorado, Boulder, CO 80309 0215, USA
    Proc Natl Acad Sci U S A 94:11190-5. 1997
    ....
  11. ncbi PTOP interacts with POT1 and regulates its localization to telomeres
    Dan Liu
    Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, 1 Baylor Plaza, Houston, TX 77030, USA
    Nat Cell Biol 6:673-80. 2004
    ..Furthermore, expression of the respective interaction domains on PTOP and POT1 alone extended telomere length in human cells. Therefore, PTOP heterodimerizes with POT1 and regulates POT1 telomeric recruitment and telomere length...
  12. ncbi Fission yeast Pot1-Tpp1 protects telomeres and regulates telomere length
    Tomoichiro Miyoshi
    Department of Gene Mechanisms, Graduate School of Biostudies, Kyoto University, Yoshida konoe cho, Sakyo ku, Kyoto 606 8501, Japan
    Science 320:1341-4. 2008
    ..Such molecular architectures are similar to those of mammalian shelterin, indicating that the overall DNA-protein architecture is conserved across evolution...
  13. pmc POT1-interacting protein PIP1: a telomere length regulator that recruits POT1 to the TIN2/TRF1 complex
    Jeffrey Zheng Sheng Ye
    Laboratory for Cell Biology and Genetics and Laboratory for Mass Spectrometry and Gaseous Ion Chemistry, The Rockefeller University, New York, New York 10021, USA
    Genes Dev 18:1649-54. 2004
    ..Reduction of PIP1 or POT1 levels with shRNAs led to telomere elongation, indicating that PIP1 contributes to telomere length control through recruitment of POT1...
  14. ncbi Early replication of short telomeres in budding yeast
    Alessandro Bianchi
    Department of Molecular Biology and NCCR Frontiers in Genetics Program, University of Geneva, Geneva, Switzerland
    Cell 128:1051-62. 2007
    ..These data reveal an epigenetic effect of telomere length on the activity of nearby replication origins and an unanticipated link between telomere replication timing and telomerase action...
  15. ncbi Interactions of putative telomere-binding proteins in Arabidopsis thaliana: identification of functional TRF2 homolog in plants
    Milan Kuchar
    Department of Functional Genomics and Proteomics, Masaryk University Brno, Kotlarska 2, CZ 611 37 Brno, Czech Republic
    FEBS Lett 578:311-5. 2004
    ..In humans, the TRF1-complex recruits hPot1 to telomeres by protein-protein interactions where it is involved in telomere length regulation. Possibly, AtTRB1 has a similar role in recruiting AtPot1...
  16. pmc Delineation of the high-affinity single-stranded telomeric DNA-binding domain of Saccharomyces cerevisiae Cdc13
    Emily M Anderson
    Department of Chemistry and Biochemistry, University of Colorado at Boulder, UCB 215, Boulder, CO 80309 0215, USA
    Nucleic Acids Res 30:4305-13. 2002
    ..The full-length protein exhibited the same rate of dissociation as both domains, however, indicating that the full-length protein contains a region that inhibits association with single-stranded telomeric DNA...
  17. pmc Array painting reveals a high frequency of balanced translocations in breast cancer cell lines that break in cancer-relevant genes
    K D Howarth
    Department of Pathology, Hutchison MRC Research Centre, University of Cambridge, Cambridge, UK
    Oncogene 27:3345-59. 2008
    ..Two gene fusions were demonstrated, TAX1BP1-AHCY and RIF1-PKD1L1. Our results support the idea that chromosome rearrangements may play an important role in common epithelial cancers such as breast cancer...
  18. pmc TRF1 is a dimer and bends telomeric DNA
    A Bianchi
    The Rockefeller University, New York, NY 10021, USA
    EMBO J 16:1785-94. 1997
    ..Furthermore, TRF1 was found to bend its telomeric site to an angle of -120 degrees. Since Rap1p similarly distorts telomeric DNA, we propose that DNA bending is important for the function of telomeres in yeast and mammals...
  19. ncbi Loss of hPot1 function leads to telomere instability and a cut-like phenotype
    Timothy Veldman
    Department of Pharmacology and Cancer Biology, Department of Radiation Oncology, Duke University Medical Center, Durham, NC 27710, USA
    Curr Biol 14:2264-70. 2004
    ..We conclude that hPot1 protects chromosome ends from illegitimate recombination, catastrophic chromosome instability, and abnormal chromosome segregation...
  20. ncbi Switching human telomerase on and off with hPOT1 protein in vitro
    Ming Lei
    Howard Hughes Medical Institute, Department of Chemistry and Biochemistry, University of Colorado, Boulder, Colorado 80309 0215, USA
    J Biol Chem 280:20449-56. 2005
    ..Thus, depending on its location relative to the DNA 3'-end, hPOT1 can either inhibit telomerase action or form a preferred substrate for telomerase. We propose that another factor catalyzes the interconversion of these states in vivo...
  21. ncbi The telomere bouquet controls the meiotic spindle
    Kazunori Tomita
    Telomere Biology Laboratory, Cancer Research UK, London WC2A 3PX, UK
    Cell 130:113-26. 2007
    ..This discovery illuminates an unanticipated level of communication between chromosomes and the spindle apparatus that may be widely conserved among eukaryotes...
  22. ncbi The POT1-TPP1 telomere complex is a telomerase processivity factor
    Feng Wang
    Department of Biological Chemistry, University of Michigan Medical School, MSRBIII 5301D, 1150 W Medical Center Drive, Ann Arbor, Michigan 48109, USA
    Nature 445:506-10. 2007
    ..We propose that POT1-TPP1 switches from inhibiting telomerase access to the telomere, as a component of shelterin, to serving as a processivity factor for telomerase during telomere extension...
  23. pmc Protection of telomeres by a conserved Stn1-Ten1 complex
    Victoria Martin
    Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Proc Natl Acad Sci U S A 104:14038-43. 2007
    ..Structural profiling studies detect OB-fold domains in Stn1 and Ten1 orthologs, indicating that protection of telomeres by multiple proteins with OB-fold domains is conserved in eukaryotic evolution...
  24. ncbi Two modes of survival of fission yeast without telomerase
    T M Nakamura
    Howard Hughes Medical Institute, Department of Chemistry and Biochemistry, University of Colorado, Boulder, CO 80309 0215, USA
    Science 282:493-6. 1998
    ..Moreover, the massive elongation of telomeres in taz1- cells is absent in the double mutant. Thus, Taz1p appears to regulate telomeric recombination as well as telomerase activity in fission yeast...
  25. pmc SUN1 and SUN2 play critical but partially redundant roles in anchoring nuclei in skeletal muscle cells in mice
    Kai Lei
    Institute of Developmental Biology and Molecular Medicine, School of Life Science, Fudan University, Shanghai, China
    Proc Natl Acad Sci U S A 106:10207-12. 2009
    ..These results clearly indicate that SUN1 and SUN2 function critically in skeletal muscle cells for Syne-1 localization at the NE, which is essential for proper myonuclear positioning...
  26. ncbi Regulation of telomerase by telomeric proteins
    Agata Smogorzewska
    The Rockefeller University, New York, New York 10021, USA
    Annu Rev Biochem 73:177-208. 2004
    ..Here we discuss the details of telomerase and its regulation by the telomere...
  27. pmc The meiotic bouquet promotes homolog interactions and restricts ectopic recombination in Schizosaccharomyces pombe
    Luther Davis
    Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109 1024, USA
    Genetics 174:167-77. 2006
    ..We discuss mechanisms by which the bouquet may prevent deleterious translocations by restricting ectopic recombination...
  28. pmc Arabidopsis POT1 associates with the telomerase RNP and is required for telomere maintenance
    Yulia V Surovtseva
    Department of Biochemistry and Biophysics, Texas A and M University, College Station, TX 77843 2128, USA
    EMBO J 26:3653-61. 2007
    ....
  29. ncbi Tel2 regulates the stability of PI3K-related protein kinases
    Hiroyuki Takai
    Laboratory for Cell Biology and Genetics, The Rockefeller University, 1230 York Avenue, New York, NY 10065, USA
    Cell 131:1248-59. 2007
    ..These data identify Tel2 as a highly conserved regulator of PIKK stability...
  30. ncbi Recent expansion of the telomeric complex in rodents: Two distinct POT1 proteins protect mouse telomeres
    Dirk Hockemeyer
    Laboratory for Cell Biology and Genetics, The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA
    Cell 126:63-77. 2006
    ..We conclude that mouse telomeres require two distinct POT1 proteins whereas human telomeres have one. Such divergence is unprecedented in mammalian chromosome biology and has implications for modeling human telomere biology in mice...
  31. pmc The human telomere-associated protein TIN2 stimulates interactions between telomeric DNA tracts in vitro
    Sahn Ho Kim
    Life Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA
    EMBO Rep 4:685-91. 2003
    ..Unlike TRF1, TIN2 did not form homodimers. We propose that TIN2 alters the conformation of TRF1, which favours a tertiary telomeric structure that hinders telomerase from gaining access to telomeres...
  32. pmc The Arabidopsis Pot1 and Pot2 proteins function in telomere length homeostasis and chromosome end protection
    Eugene V Shakirov
    Department of Biochemistry and Biophysics, Texas A and M University, College Station, TX 77843 2128, USA
    Mol Cell Biol 25:7725-33. 2005
    ..Thus, Arabidopsis has evolved two Pot genes that function differently in telomere biology. These findings provide unanticipated information about the evolution of single-stranded telomere binding proteins.
  33. pmc The Est1 subunit of Saccharomyces cerevisiae telomerase makes multiple contributions to telomere length maintenance
    Sara K Evans
    Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030, USA
    Genetics 162:1101-15. 2002
    ..The isolation of mutations that perturb separate functions of Est1 demonstrates that a telomerase holoenzyme subunit can contribute multiple regulatory roles to telomere length maintenance...
  34. pmc Human POT1 disrupts telomeric G-quadruplexes allowing telomerase extension in vitro
    Arthur J Zaug
    Howard Hughes Medical Institute and Department of Chemistry and Biochemistry, University of Colorado, Boulder, CO 80309 0215, USA
    Proc Natl Acad Sci U S A 102:10864-9. 2005
    ..We propose an additional role for hPOT1 in telomere maintenance: disrupting G-quadruplex structures in telomeric DNA, thereby allowing proper elongation by telomerase...
  35. ncbi A RAP1/TRF2 complex inhibits nonhomologous end-joining at human telomeric DNA ends
    Nancy S Bae
    Stowers Institute for Medical Research, Kansas City, MO 64110, USA
    Mol Cell 26:323-34. 2007
    ..Biochemical fractionation and reconstitution revealed that telomere protection is mediated by a RAP1/TRF2 complex, providing evidence for a direct role for human RAP1 in the protection of telomeric DNA from NHEJ...
  36. pmc Interaction of Saccharomyces Cdc13p with Pol1p, Imp4p, Sir4p and Zds2p is involved in telomere replication, telomere maintenance and cell growth control
    Chia Ling Hsu
    Institute of Biopharmaceutical Science, National Yang Ming University, Shih Pai 112, Taipei, Taiwan, Republic of China
    Nucleic Acids Res 32:511-21. 2004
    ..Together, our results provide evidence for the formation of a Cdc13p-mediated telosome complex through its N-terminal region that is involved in telomere maintenance, telomere length regulation and cell growth control...
  37. pmc Rap1 prevents telomere fusions by nonhomologous end joining
    Benjamin Pardo
    Laboratoire de Radiobiologie de l ADN, Service de Radiobiologie Moléculaire et Cellulaire, CEA Fontenay, Fontenay aux Roses, France
    EMBO J 24:3117-27. 2005
    ..Together, these results show that Rap1 is essential to block NHEJ between telomeres. Since the presence of Rap1 at telomeres has been conserved through evolution, the establishment of NHEJ suppression by Rap1 could be universal...
  38. pmc Two modes of DNA double-strand break repair are reciprocally regulated through the fission yeast cell cycle
    Miguel Godinho Ferreira
    Telomere Biology Laboratory, Cancer Research UK, London WC2A 3PX, UK
    Genes Dev 18:2249-54. 2004
    ..Whereas NHEJ is known to be dispensable for survival of DSBs in cycling cells, we find that it is critical for repair and survival of DSBs arising during G1...
  39. pmc Mechanisms that regulate localization of a DNA double-strand break to the nuclear periphery
    Pranav Oza
    Program in Molecular Medicine, Interdisciplinary Graduate Program, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
    Genes Dev 23:912-27. 2009
    ..Based on these findings, we propose that sequestration of unrepaired or slowly repaired DSBs to the nuclear periphery reflects a competition between alternative repair pathways...
  40. pmc Abrogation of the CLK-2 checkpoint leads to tolerance to base-excision repair intermediates
    Marlene Dengg
    The Biotechnology Centre, University of Oslo, PO Box 1125 Blindern, Gaustadalleen 21, 0317 Oslo, Norway
    EMBO Rep 7:1046-51. 2006
    ..Our results indicate that persistent repair intermediates and/or single-stranded DNA formed during repair of misincorporated uracil are tolerated in the absence of the CLK-2 checkpoint in C. elegans...
  41. ncbi Identification of human Rap1: implications for telomere evolution
    B Li
    The Rockefeller University, New York, New York 10021, USA
    Cell 101:471-83. 2000
    ..We propose that budding yeast preserved Rap1 at telomeres but lost the TRF component, possibly concomitant with a change in the telomeric repeat sequence...
  42. pmc Inhibition of yeast telomerase action by the telomeric ssDNA-binding protein, Cdc13p
    David C Zappulla
    Howard Hughes Medical Institute, University of Colorado, Boulder, CO 80309, USA
    Nucleic Acids Res 37:354-67. 2009
    ....
  43. pmc Human RAP1 inhibits non-homologous end joining at telomeres
    Jay Sarthy
    Stowers Institute for Medical Research, Kansas City, MO 64110, USA
    EMBO J 28:3390-9. 2009
    ..These results provide the first evidence that hRAP1 inhibits NHEJ at mammalian telomeres and identify hRAP1 as a mediator of genome stability...
  44. ncbi Evidence for alternative lengthening of telomeres in liposarcomas in the absence of ALT-associated PML bodies
    Jennie N Jeyapalan
    Department of Genetics, University of Leicester, Leicester LE1 7RH, United Kingdom
    Int J Cancer 122:2414-21. 2008
    ..This has major implications for the use of cancer treatments targeted at TMMs...
  45. ncbi Tel2 is required for activation of the Mrc1-mediated replication checkpoint
    Miho Shikata
    Department of Gene Mechanisms, Graduate School of Biostudies, Kyoto University, Kitashirakawaoiwake cho, Sakyo ku, Kyoto 606 8502, Japan
    J Biol Chem 282:5346-55. 2007
    ..These results indicate that S. pombe Tel2 plays important roles in the Mrc1-mediated replication checkpoint as well as in the Cds1-independent regulation of genome integrity...
  46. ncbi A new model for Schizosaccharomyces pombe telomere recognition: the telomeric single-stranded DNA-binding activity of Pot11-389
    Johnny E Croy
    Department of Chemistry and Biochemistry, University of Colorado, Boulder, 80309 0215, USA
    J Mol Biol 361:80-93. 2006
    ..The biochemical behavior of Pot1(1-389) is more similar to that of the full-length SpPot1 protein than to that of Pot1pN, making Pot1(1-389) a valuable domain for the future study of how full-length SpPot1 interacts with telomeric ssDNA...
  47. ncbi A dynamic molecular link between the telomere length regulator TRF1 and the chromosome end protector TRF2
    Benjamin R Houghtaling
    Skirball Institute of Biomolecular Medicine, New York University School of Medicine, 540 First Avenue, New York, NY 10016, USA
    Curr Biol 14:1621-31. 2004
    ..Human telomeres are coated by the telomere repeat binding proteins TRF1 and TRF2, which are believed to function independently to regulate telomere length and protect chromosome ends, respectively...
  48. ncbi TIN2 is a tankyrase 1 PARP modulator in the TRF1 telomere length control complex
    Jeffrey Zheng Sheng Ye
    The Rockefeller University, 1230 York Avenue, New York, New York 10021, USA
    Nat Genet 36:618-23. 2004
    ..These data identify TIN2 as a PARP modulator in the TRF1 complex and can explain how TIN2 contributes to the regulation of telomere length...
  49. ncbi Pot1, the putative telomere end-binding protein in fission yeast and humans
    P Baumann
    Howard Hughes Medical Institute, Department of Chemistry and Biochemistry, University of Colorado, Boulder, CO 80309, USA
    Science 292:1171-5. 2001
    ..It now appears that the protein that caps the ends of chromosomes is widely dispersed throughout the eukaryotic kingdom...
  50. ncbi Lcd1p recruits Mec1p to DNA lesions in vitro and in vivo
    John Rouse
    Wellcome Trust and Cancer Research UK, Institute of Cancer and Developmental Biology, University of Cambridge, Cambridge CB2 1QR, United Kingdom
    Mol Cell 9:857-69. 2002
    ..Recruitment of Lcd1p to these lesions is independent of Mec1p and Rad9p/Rad24p. Thus, recruitment of Mec1p to DNA lesions by Lcd1p is crucial for the DNA damage response...
  51. ncbi Conserved structure for single-stranded telomeric DNA recognition
    Rachel M Mitton-Fry
    Department of Chemistry and Biochemistry, University of Colorado, Boulder, CO 80309, USA
    Science 296:145-7. 2002
    ..The common usage of an OB fold for telomeric DNA interaction demonstrates conservation of end-protection mechanisms among eukaryotes...
  52. ncbi POT1 stimulates RecQ helicases WRN and BLM to unwind telomeric DNA substrates
    Patricia L Opresko
    Laboratory of Molecular Gerontology, National Institute on Aging, Baltimore, Maryland 21224, USA
    J Biol Chem 280:32069-80. 2005
    ..We propose roles for the cooperation of POT1 with RecQ helicases WRN and BLM in resolving DNA structures at telomeric ends, in a manner that protects the telomeric 3' tail as it is exposed during unwinding...
  53. ncbi DNA self-recognition in the structure of Pot1 bound to telomeric single-stranded DNA
    Ming Lei
    Howard Hughes Medical Institute, Department of Chemistry and Biochemistry, University of Colorado, Boulder, Colorado 80309 0215, USA
    Nature 426:198-203. 2003
    ..The structure also explains how Pot1p avoids binding the vast excess of RNA in the nucleus...
  54. pmc Increased genome instability and telomere length in the elg1-deficient Saccharomyces cerevisiae mutant are regulated by S-phase checkpoints
    Soma Banerjee
    Genome Instability Section, Genetics and Molecular Biology Branch, National Human Genome Research Institute, National Institutes of Health, Building 49, Room 4A22, Bethesda, MD 20892, USA
    Eukaryot Cell 3:1557-66. 2004
    ....
  55. pmc Transmembrane protein Sun2 is involved in tethering mammalian meiotic telomeres to the nuclear envelope
    Johannes Schmitt
    Department of Cell and Developmental Biology, Biocenter of the University of Wurzburg, D 97074 Wurzburg, Germany
    Proc Natl Acad Sci U S A 104:7426-31. 2007
    ..Together with recent findings in fission yeast, our study indicates that the molecular mechanisms required for tethering meiotic telomeres and their dynamic movements during bouquet formation are conserved among eukaryotes...
  56. pmc Cdk1-dependent phosphorylation of Cdc13 coordinates telomere elongation during cell-cycle progression
    Shang Li
    Department of Biochemistry and Biophysics, University of California, San Francisco, Box 2200, San Francisco, CA 94143 2200, USA
    Cell 136:50-61. 2009
    ..These results provide a direct mechanistic link between coordination of telomere elongation and cell-cycle progression in vivo...
  57. ncbi Extended DNA binding site in Pot1 broadens sequence specificity to allow recognition of heterogeneous fission yeast telomeres
    Kelly M Trujillo
    Stowers Institute for Medical Research, Kansas City, Missouri 64110, USA
    J Biol Chem 280:9119-28. 2005
    ..Consistent with a role in chromosome end capping, Pot1 prevents access of telomerase to the 3'-end and protects against exonucleolytic degradation...
  58. pmc Yeast cell death during DNA damage arrest is independent of caspase or reactive oxygen species
    Robert Wysocki
    Center for Molecular Oncology, The University of Chicago, 924 E 57th Street, Rm R320, IL 60637, USA
    J Cell Biol 166:311-6. 2004
    ..We speculate that during a prolonged cell cycle arrest, cdc13-1 cells reach a critical size and die by cell lysis...
  59. ncbi Structure of human POT1 bound to telomeric single-stranded DNA provides a model for chromosome end-protection
    Ming Lei
    Howard Hughes Medical Institute, Department of Chemistry and Biochemistry, University of Colorado, Boulder, Colorado 80309 0215, USA
    Nat Struct Mol Biol 11:1223-9. 2004
    ..pombe Pot1pN-ssDNA complex, whereas the second OB fold binds and protects the 3' end of the ssDNA. These results provide an atomic-resolution model for chromosome end-capping...
  60. ncbi Prediction of multiple tandem OB-fold domains in telomere end-binding proteins Pot1 and Cdc13
    Douglas L Theobald
    Department of Chemistry and Biochemistry, University of Colorado at Boulder, Boulder, CO 80309, USA
    Structure 12:1877-9. 2004
    ..This common usage of multiple, tandem OB-fold domains in telomeric end binding proteins extends the known evolutionary conservation of eukaryotic end-protection mechanisms...
  61. pmc The Rad51 pathway of telomerase-independent maintenance of telomeres can amplify TG1-3 sequences in yku and cdc13 mutants of Saccharomyces cerevisiae
    Nathalie Grandin
    Ecole Normale Superieure de Lyon, UMR CNRS 5665, 69364 Lyon, France
    Mol Cell Biol 23:3721-34. 2003
    ..They also suggest that Rad51 is more efficient than Rad50 in amplifying the sequences left uncovered by the absence of Cdc13 or Yku70...
  62. pmc Meiotic telomere clustering requires actin for its formation and cohesin for its resolution
    Edgar Trelles-Sticken
    Max Planck Institute for Molecular Genetics, D 14195 Berlin, Germany
    J Cell Biol 170:213-23. 2005
    ..Our results suggest that cohesin is required for exit from actin polymerization-dependent telomere clustering and for linking the SPB to the telomere cluster in synaptic meiosis...
  63. pmc Dysfunctional telomeres activate an ATM-ATR-dependent DNA damage response to suppress tumorigenesis
    Xiaolan Guo
    Department of Cancer Genetics, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA
    EMBO J 26:4709-19. 2007
    ..In addition, our results suggest that loss of ATM can cooperate with dysfunctional telomeres to promote cellular transformation and tumor formation in vivo...
  64. pmc A critical role for TPP1 and TIN2 interaction in high-order telomeric complex assembly
    Matthew S O'Connor
    Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA
    Proc Natl Acad Sci U S A 103:11874-9. 2006
    ..Our results suggest that coordinated interactions among TPP1, TIN2, TRF1, and TRF2 may ensure robust assembly of the telosome, telomere targeting of its subunits, and, ultimately, regulated telomere maintenance...
  65. ncbi Semi-conservative DNA replication through telomeres requires Taz1
    Kyle M Miller
    Telomere Biology Laboratory, Cancer Research UK, 44 Lincoln s Inn Fields, London WC2A 3PX, UK
    Nature 440:824-8. 2006
    ..Stalled forks at dysfunctional human telomeres are likely to accelerate the genomic instability that drives tumorigenesis...
  66. pmc Senescence mutants of Saccharomyces cerevisiae with a defect in telomere replication identify three additional EST genes
    T S Lendvay
    Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030, USA
    Genetics 144:1399-412. 1996
    ....
  67. pmc Rap1p and other transcriptional regulators can function in defining distinct domains of gene expression
    Qun Yu
    Department of Biochemistry, University of Nebraska Lincoln, Lincoln, NE 68588, USA
    Nucleic Acids Res 31:1224-33. 2003
    ..Moreover, targeting the activation domain of Adr1p or Gcn4p also establishes a barrier to silencing. These results support the notion that transcriptional regulators could also participate in delimiting functional domains in the genome...
  68. ncbi A shared docking motif in TRF1 and TRF2 used for differential recruitment of telomeric proteins
    Yong Chen
    Department of Biological Chemistry, University of Michigan Medical School, 1150 West Medical Center Drive, Ann Arbor, MI 48109, USA
    Science 319:1092-6. 2008
    ..Conversely, the TRFH domain of TRF1, but not of TRF2, interacts with another shelterin-associated factor: PinX1...
  69. ncbi The maintenance and masking of chromosome termini
    Alison A Bertuch
    Department of Pediatrics, Hematology Oncology Section, Baylor College of Medicine, Houston, TX 77030, USA
    Curr Opin Cell Biol 18:247-53. 2006
    ....
  70. pmc Human protection of telomeres 1 (POT1) is a negative regulator of telomerase activity in vitro
    Colleen Kelleher
    ISREC, Chemin des Boveresses 155, 1066 Epalinges, Switzerland
    Mol Cell Biol 25:808-18. 2005
    ..Furthermore, POT1 is incapable of inhibiting telomeric repeat addition to substrate primers that are defective for POT1 binding, suggesting that in vivo, POT1 likely affects substrate access to telomerase...
  71. ncbi Telomere biology in neuroblastoma: telomere binding proteins and alternative strengthening of telomeres
    Yoshiyuki Onitake
    Department of Surgery, Graduate School of Biomedical Science, Hiroshima University, Hiroshima 734 8551, Japan
    J Pediatr Surg 44:2258-66. 2009
    ..Recently, telomere binding proteins (TBPs) and alternative lengthening of telomeres (ALTs) have been identified as key factors of telomere ..
  72. ncbi RPA-like mammalian Ctc1-Stn1-Ten1 complex binds to single-stranded DNA and protects telomeres independently of the Pot1 pathway
    Yasuyuki Miyake
    Laboratory of Cell Cycle Regulation, Department of Gene Mechanisms, Graduate School of Biostudies, Kyoto University, Yoshida konoe cho, Sakyo ku, Kyoto 606 8501, Japan
    Mol Cell 36:193-206. 2009
    ..We propose that CST is a replication protein A (RPA)-like complex that is not directly involved in conventional DNA replication at forks but plays a role in DNA metabolism frequently required by telomeres...
  73. pmc Multiple pathways inhibit NHEJ at telomeres
    Stéphane Marcand
    Centre National de la Recherche Scientifque UMR 217, Institut de Radiobiologie Cellulaire et Moleculaire, CEA Fontenay, 92265 Fontenay aux Roses Cedex, France
    Genes Dev 22:1153-8. 2008
    ..We discuss a possible mechanism that would explain Rif2 multifunctionality at telomeres and the recent evolutionary origin of Rif2 from an origin recognition complex (ORC) subunit...
  74. pmc Fission yeast mutants affecting telomere clustering and meiosis-specific spindle pole body integrity
    Ye Jin
    Department of Molecular and Cell Biology, University of California, Berkeley, California 94732, USA
    Genetics 160:861-76. 2002
    ..The coincidence of impaired SPB functions with defective telomere clustering suggests a link between the SPB and the telomere cluster...
  75. pmc Conserved telomere maintenance component 1 interacts with STN1 and maintains chromosome ends in higher eukaryotes
    Yulia V Surovtseva
    Department of Biochemistry and Biophysics, Texas A and M University, 2128 TAMU, College Station, TX 77843 2128, USA
    Mol Cell 36:207-18. 2009
    ..These data indicate that CTC1 participates in telomere maintenance in diverse species and that a CST-like complex is required for telomere integrity in multicellular organisms...
  76. pmc Rescue of an hTERT mutant defective in telomere elongation by fusion with hPot1
    Blaine N Armbruster
    Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, North Carolina 27710, USA
    Mol Cell Biol 24:3552-61. 2004
    ..However, this rescue depended on the ability of hPot1 to localize to telomeres rather than binding to DNA per se. These data support a model whereby the DAT domain of hTERT is implicated in telomere-telomerase associations...
  77. pmc POT1 and TRF2 cooperate to maintain telomeric integrity
    Qin Yang
    Laboratory of Human Carcinogenesis, National Cancer Institute NIH, Bldg 37, Rm 3068, 37 Convent Drive, Bethesda, MD 20892 4255, USA
    Mol Cell Biol 25:1070-80. 2005
    ..These results demonstrate that POT1 and TRF2 share in part in the same pathway for telomere capping and suggest that POT1 binds to the telomeric single-stranded DNA in the D-loop and cooperates with TRF2 in t-loop maintenance...
  78. pmc Sir proteins, Rif proteins, and Cdc13p bind Saccharomyces telomeres in vivo
    B D Bourns
    Pathology Department, University of Washington, Seattle, Washington 98195, USA
    Mol Cell Biol 18:5600-8. 1998
    ..We describe a one-hybrid assay for telomere binding proteins and use it to establish that six proteins that affect telomere structure or function but which had not ..
  79. pmc Distinct roles for yeast Stn1 in telomere capping and telomerase inhibition
    Andrea Puglisi
    Department of Molecular Biology and NCCR Program Frontiers in Genetics, University of Geneva, Geneva, Switzerland
    EMBO J 27:2328-39. 2008
    ..These data provide new insights into the coordination of telomere capping and telomerase regulation...
  80. pmc Taz1, Rap1 and Rif1 act both interdependently and independently to maintain telomeres
    Kyle M Miller
    Cancer Research UK, London Research Institute, London, UK
    EMBO J 24:3128-35. 2005
    ..Thus, Rap1 and Rif1 are each required for a subset of the functions of Taz1, but each acquires Taz1-independent functions in its absence. Furthermore, Taz1 can function independently of its known binding partners...
  81. ncbi Getting to the end: telomerase access in yeast and humans
    Leticia R Vega
    Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544, USA
    Nat Rev Mol Cell Biol 4:948-59. 2003
  82. pmc A genomewide suppressor and enhancer analysis of cdc13-1 reveals varied cellular processes influencing telomere capping in Saccharomyces cerevisiae
    S G Addinall
    School of Computing Science, Newcastle University, Newcastle upon Tyne NE1 7RU, United Kingdom
    Genetics 180:2251-66. 2008
    ..The high-throughput spot-testing approach that we describe is generally applicable and could aid in understanding other aspects of eukaryotic cell biology...
  83. pmc Human Rif1 protein binds aberrant telomeres and aligns along anaphase midzone microtubules
    Lifeng Xu
    Department of Biochemistry and Biophysics, University of California, San Francisco, CA 94143, USA
    J Cell Biol 167:819-30. 2004
    ..In telophase, hRif1 localized to chromosomes, and in interphase, it was intranuclear. These results define a novel subcellular localization behavior for hRif1 during the cell cycle...
  84. ncbi Genetic variation, nucleotide diversity, and linkage disequilibrium in seven telomere stability genes suggest that these genes may be under constraint
    Sharon A Savage
    Section on Genomic Variation, Pediatric Oncology Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892 4605, USA
    Hum Mutat 26:343-50. 2005
    ..These data establish a foundation for further investigation of these genes in population-genetics, evolution, and disease-association studies...
  85. ncbi Telomere protection by mammalian Pot1 requires interaction with Tpp1
    Dirk Hockemeyer
    Laboratory for Cell Biology and Genetics, The Rockefeller University, 1230 York Avenue, New York, New York 10065, USA
    Nat Struct Mol Biol 14:754-61. 2007
    ....
  86. pmc Conservation of telomere protein complexes: shuffling through evolution
    Benjamin R Linger
    Department of Cancer and Cell Biology, University of Cincinnati, Cincinnati, OH 45267 0521, USA
    Crit Rev Biochem Mol Biol 44:434-46. 2009
    ..While one paralog may be part of a conserved telomere protein complex and have the expected function, the other paralog may serve in a completely different aspect of telomere biology...
  87. ncbi POT1-independent single-strand telomeric DNA binding activities in Brassicaceae
    Eugene V Shakirov
    Department of Biochemistry and Biophysics, Texas A and M University, 2128 TAMU, College Station, Texas 77843 2128, USA
    Plant J 58:1004-15. 2009
    ..thaliana and its close relatives, underscoring the remarkable functional divergence of POT1 proteins from plants and other eukaryotes...
  88. ncbi Arabidopsis POT1A interacts with TERT-V(I8), an N-terminal splicing variant of telomerase
    Pascale Rossignol
    Department of Cellular and Developmental Biology, John Innes Centre, Norwich, NR4 7UH, UK
    J Cell Sci 120:3678-87. 2007
    ..The interaction is specific to the N-terminal region of the telomerase, which can be encoded by splicing variants. This interaction indicates possible mechanisms for telomerase regulation by alternative splicing and by POT1 proteins...
  89. pmc Human POT1 is required for efficient telomere C-rich strand replication in the absence of WRN
    Nausica Arnoult
    Telomeres and Cancer Laboratory, Institut Curie, Paris 75248, France
    Genes Dev 23:2915-24. 2009
    ..Our study reveals an unanticipated role for hPOT1 during telomere replication...
  90. ncbi The transcription factor Ifh1 is a key regulator of yeast ribosomal protein genes
    Joseph T Wade
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Nature 432:1054-8. 2004
    ..Thus, Ifh1 association with promoters is the key regulatory step for coordinate expression of RP genes...
  91. pmc Fission yeast Taz1 and RPA are synergistically required to prevent rapid telomere loss
    Tatsuya Kibe
    Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, Higashi Hiroshima 739 8530, Japan
    Mol Biol Cell 18:2378-87. 2007
    ..Our results suggest that controlling the activity of Rqh1 at telomeres is critical for the prevention of genomic instability...
  92. pmc Probing the mechanism of recognition of ssDNA by the Cdc13-DBD
    Aimee M Eldridge
    Department of Chemistry and Biochemistry, University of Colorado, Boulder, CO 80309 0215, USA
    Nucleic Acids Res 36:1624-33. 2008
    ..These data point to an overall binding mechanism well adapted to the heterogeneous nature of yeast telomeres...
  93. ncbi Telomeres: cancer to human aging
    Sheila A Stewart
    Departments of Cell Biology and Physiology and of Medicine, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Annu Rev Cell Dev Biol 22:531-57. 2006
    ..Here we discuss how telomeres are monitored and maintained and how loss of a functional telomere influences biological functions as diverse as aging and carcinogenesis...
  94. ncbi Telomeres in evolution and evolution of telomeres
    Jiri Fajkus
    Laboratory of Functional Genomics and Proteomics, Faculty of Science, Masaryk University Brno, Kralovopolska 135, CZ 61265 Brno, Czech Republic
    Chromosome Res 13:469-79. 2005
    ..We describe how telomere dysfunction, resulting in end-to-end chromosome fusions, can have a profound effect on chromosome evolution and perhaps even speciation...
  95. ncbi ssDNA fragments induce cell senescence by telomere uncapping
    Avgi Tsolou
    Centre for Integrated Systems Biology of Ageing and Nutrition, Institute for Ageing and Health, Newcastle University, Newcastle upon Tyne, UK
    Exp Gerontol 43:892-9. 2008
    ..Together, our results show that short G-rich single-stranded oligonucleotides induce telomere uncapping in a cell cycle-dependent manner, probably by titrating essential factors like Pot1 away from telomeres...
  96. pmc Higher-order nuclear organization in growth arrest of human mammary epithelial cells: a novel role for telomere-associated protein TIN2
    Patrick Kaminker
    Buck Institute for Age Research, 8001 Redwood Boulevard, Novato, California 94945, USA
    J Cell Sci 118:1321-30. 2005
    ..Here we show that a novel extra-telomeric organization of TIN2 is associated with the control of cell proliferation and identify TIN2 as an important regulator of mammary epithelial differentiation...
  97. ncbi Alternative splicing of Pot1 (Protection of telomere)-like genes in Arabidopsis thaliana
    Akinori Tani
    Research Institute for Bioresources, Okayama University Kurashiki, Japan
    Genes Genet Syst 80:41-8. 2005
    ..By western blot analysis, we found that the antibody made against the N-terminal amino acids of AtPOT1-1 recognized three different polypeptides, indicating that all three variants are being translated in Arabidopsis...
  98. pmc Ndj1, a telomere-associated protein, promotes meiotic recombination in budding yeast
    Hsin Yen Wu
    Section of Molecular and Cellular Biology, Genetics Graduate Program, University of California, Davis, California, USA
    Mol Cell Biol 26:3683-94. 2006
    ..These findings provide a link between telomere dynamics and a distinct mechanistic step of meiotic recombination that follows the homology search...
  99. pmc POT1 protects telomeres from a transient DNA damage response and determines how human chromosomes end
    Dirk Hockemeyer
    Laboratory for Cell Biology and Genetics, The Rockefeller University, New York, NY 10021, USA
    EMBO J 24:2667-78. 2005
    ....
  100. pmc Regulation of telomere length by an N-terminal region of the yeast telomerase reverse transcriptase
    Hong Ji
    Vanderbilt University, Department of Biological Sciences, VU Station B, Nashville, TN 37235, USA
    Mol Cell Biol 25:9103-14. 2005
    ..Taken together, these data suggest that specific amino acids within region I of the catalytic subunit of yeast telomerase play a previously unanticipated role in the response to Tel1p regulation at the telomere...
  101. pmc Functional dissection of human and mouse POT1 proteins
    Wilhelm Palm
    Laboratory for Cell Biology and Genetics, The Rockefeller University, 1230 York Avenue, New York, NY 10065 6399, USA
    Mol Cell Biol 29:471-82. 2009
    ..Thus, human POT1 combines the features of POT1a and POT1b...

Research Grants52

  1. Regulation of EBV Latency by EBNA1
    Paul M Lieberman; Fiscal Year: 2013
    ..More detailed understanding of EBNA1 is further justified by its potential value as a target for therapeutic intervention in diseases and cancers associated with EBV latent infection. ..
  2. Telomere and its bio-regulators as predictors for clinical diabetes in women
    Simin Liu; Fiscal Year: 2010
    ..and endothelial dysfunction, as well as single nucleotide polymorphisms (SNPs) on the genes of telomere binding proteins and telomerase maintenance, with telomere length in relation to the development of T2DM in these well-..
  3. Studying a Human Stem Cell Disease Using iPS Technology
    Steven E Artandi; Fiscal Year: 2010
    ..DC is caused by mutations in telomerase genes or genes encoding telomere binding proteins. The accelerated telomere shortening that occurs in DC impairs tissue stem cell function, making DC a ..
  4. Nucleoprotein Structures at Telomeres and Sites of DNA Damage
    Jack D Griffith; Fiscal Year: 2013
    ..In AIM I, the core telomere binding proteins (TRF1, TRF2, Pot1, TPP1, hRap1, and Tin2) highly purified and in hand together with co-complexes of ..
  5. Telomerace Recruitment to Telomeres Via Core Telomore Binding Proteins
    Eladio Abreu; Fiscal Year: 2010
    ..during S-phase of the cell cycle and is facilitated by the dynamic association of the enzyme with core telomere binding proteins. Fluorescence microscopy techniques developed by our lab will be employed in a variety of assays to ..
  6. STRUCTURE AND BEHAVIOR OF YEAST TELOMERES
    Virginia A Zakian; Fiscal Year: 2013
    ..TG1-3 tails are required to form a stable holoenzyme-telomere complex and if certain mutations in telomere binding proteins and/or telomerase components are defective in this step...
  7. Control of Telomere Heterochromatin by TERRA
    Paul M Lieberman; Fiscal Year: 2013
    ..The long-term goal of this proposal is to determine how telomeric heterochromatin is deregulated in cellular aging and cancer. ..
  8. Investigation into Mechanisms of CDK1 in Controlling Telomere Stability
    Weihang Chai; Fiscal Year: 2013
    ....
  9. Processing of Alternative Structures in Telomeric DNA
    GERALD JOSEPH NORA; Fiscal Year: 2011
    ..This proposal analyzes the mechanisms for telomeric DNA processing, which should provide insights into the aging process and may assist in finding therapeutic targets to arrest or delay genomic instability and telomere loss. ..
  10. Surveillance and maintenance of DNA ends
    KATSONURI SUGIMOTO; Fiscal Year: 2013
    ....
  11. GENE EXPRESSION IN AGING AND DEVELOPMENT
    WOODRING ERIK WRIGHT; Fiscal Year: 2012
    ..ends of chromosomes) are composed of many kilobases of the repetitive sequence TTAGGG that together with telomere binding proteins prevent the cell from recognizing the ends as DNA breaks needing repair...
  12. Research supplement to promote diversity/Towards a comprehensive protein interact
    Haiyuan Yu; Fiscal Year: 2013
    ..and chromodomain heterochromatin proteins, gene and transposon regulation by the RNAi pathway, telomere binding proteins, and the presence of introns in most genes, S. pombe is more similar to metazoans than is S. cerevisiae...
  13. HYDROLYTIC AND FREE RADICAL MEDIATED DNA DAMAGE
    Lawrence C Sowers; Fiscal Year: 2012
    ..The studies proposed here will lead to a better understanding of cancer etiology and suggest new strategies for cancer chemotherapy. ..
  14. DNA damage induced structural and dynamic changes at telomeres
    Hong Wang; Fiscal Year: 2013
    ..such as UV light or oxidative stress) at telomeres can cause stochastically unstable assemblies of telomere binding proteins on DMA...
  15. Mechanisms of Telomere Resistance to DNA Lesion Removal
    PATRICIA LYNN OPRESKO; Fiscal Year: 2013
    ..This proposal will test the hypothesis that telomere binding proteins prevent DNA lesion removal at telomeres by inhibiting the enzymatic activities of NER enzymes...
  16. The Role of Structural Dynamics on Telomeric End Accessibility
    Helen Hwang; Fiscal Year: 2013
    ..We aim to investigate how telomeric overhang conformation is related to telomerase activity and how telomere binding proteins can modulate overhang dynamics and telomere extension...
  17. DNA REPLICATION AND CHROMOSOME STRUCTURE IN YEAST
    Virginia Zakian; Fiscal Year: 2009
    ..Rif Ip and Rif2p are telomere binding proteins that act synergistically to limit telomere lengthening...
  18. Determinants of Telomerase-Negative Cancer Cells
    Sahn Ho Kim; Fiscal Year: 2005
    ..There are no early detection markers for the ALT phenotype. Two duplex telomere binding proteins, TRF1 and TRF2, have been identified in humans. TRF1 is a negative regulator of telomere length...
  19. TELOMERE REPLICATION AND SENESCENCE IN YEAST
    Victoria Lundblad; Fiscal Year: 2001
    ..Since Est1p and Cdc13p are both single-strand telomere binding proteins, the PI proposes that these two proteins function to mediate access of telomerase to the chromosomal ..
  20. TELOMERASE AND TELOMERE DYNAMICS IN CANCER AND AGING
    Calvin Harley; Fiscal Year: 2000
    ..These include the cloning of the telomerase RNA and protein components, telomerase associated proteins, telomere binding proteins, telomerase and telomere assays, and the discovery of both positive and negative regulators of telomere ..
  21. Genomic Instability and Senescence in Cancer
    Lynne Elmore; Fiscal Year: 2009
    ..focusing on the possibility of a telomere length-independent "capping" event and assessing the role of telomere binding proteins in maintenance of genomic stability and overall telomere length/function...
  22. KU, TELOMERE MAINTENANCE AND CELLULAR SENESCENCE
    David Chen; Fiscal Year: 2004
    ..at the mammalian telomere using in vivo and in vitro experimental approaches; 2) whether known mammalian telomere binding proteins mediate an interaction between Ku and the telomere; and 3) telomere maintenance in embryonic stem cells ..
  23. The role of the telomere capping protein POT1 in mammalian aging
    Sandy Chang; Fiscal Year: 2009
    ..Given increasing evidence that telomere attrition reduces lifespan in humans, we believe our findings will be relevant to human aging as well. ..
  24. Telomeric Capping Complex of Drosophila Chromosomes
    Harald Biessmann; Fiscal Year: 2003
    ..has been worked out in sufficient detail, but very little is known about its chromatin structure and the telomere binding proteins. We propose that there are two distinct protein complexes located at Drosophila telomeres: an end-..
  25. DNA damage induced structural and dynamic changes at telomeres
    Hong Wang; Fiscal Year: 2010
    ..such as UV light or oxidative stress) at telomeres can cause stochastically unstable assemblies of telomere binding proteins on DNA...
  26. Telomere Biology and DNA Repair
    Andrew Robertson; Fiscal Year: 2009
    ..role of DNA repair proteins that are present at normally functioning telomeres, and the possible role of telomere binding proteins elsewhere in the genome are of particular interest...
  27. Structural Analysis of Telomere Chromatin
    Asmaa Baker; Fiscal Year: 2007
    ..A variety of proteins are involved in this protective structure including telomere binding proteins as well as DNA repair proteins...
  28. CONFERENCE ON TELOMERES AND TELOMERASE
    TERRI GRODZICKER; Fiscal Year: 1999
    ..In the past few years there have been major advances in our understanding of the roles of telomerase, telomere binding proteins, recombination, and telomere processing activities that affect telomere length...
  29. HYPOTRICH GENOME IN DEVELOPMENT AND EVOLUTION
    David Prescott; Fiscal Year: 2001
    ..The experiments deal with the general problem of how an organism can manipulate its own DNA. ..
  30. BCR-ABL Kinase Assays for STI571 Sensitivity of Response
    STEPHEN KRON; Fiscal Year: 2006
    ..By these aims we intend to develop a highly versatile kinase assay system which can be applied to monitoring of patient response to IM and as a tool for discovery and testing of new TKI cancer drugs. ..
  31. The acd mouse: a model for congenital adrenal hypoplasia
    Catherine Keegan; Fiscal Year: 2006
    ..Ultimately, through this additional training I will be poised to continue my long-term career goal of becoming an Independent Investigator. ..
  32. Function of the p53 tumor-suppressor in C.elegans
    JOEL ROTHMAN; Fiscal Year: 2006
    ....
  33. Cellular Lifespan and the Telomere Proteome
    Sheila Stewart; Fiscal Year: 2005
    ..These latter studies will allow us to not only identify telomere binding proteins, but will also allow us to uncover critical components of the signal transduction pathways that impinge ..
  34. New Models of Human Cancer
    William Hahn; Fiscal Year: 2005
    ..Robert Weinberg and the unique resources of the Whitehead Institute to develop these new systems and to broaden my research experiences as I make the crucial transition to an independent investigator. ..
  35. BLASTOMERE AND GERM LAYER IDENTITY IN C ELEGANS
    JOEL ROTHMAN; Fiscal Year: 2003
    ..Moreover, they will examine how dividing cells select unique pathways of differentiation, thereby helping to understand how normal controls of proliferation and differentiation are altered in cancers. ..
  36. Hindbrain Dysgenesis in Rett Syndrome and Other Autism-Spectrum Disorders
    Susan Swanberg; Fiscal Year: 2008
    ..Elucidating gene expression networks for MeCP2 targets and MeCP2 regulators may aid in the earlier diagnosis and treatment of this devastating class of neurodevelopmental disorders. [unreadable] [unreadable]..
  37. BIOCHEMICAL GENETIC STUDIES OF CELL SURVIVAL PATHWAYS
    Zhou Songyang; Fiscal Year: 2005
    ..The specific aims of this proposal are to: 1. Investigate the role of Akt in hematopoietic survival using primary cells and in chimera mice. 2. Elucidate the molecular mechanisms of how Akt mediates cell survival. ..
  38. TELOMERASE RNP STRUCTURE/FUNCTION
    DOROTHY SHIPPEN; Fiscal Year: 2001
    ..These studies will generate new insight into the structure and function of telomerase and provide needed information regarding telomerase regulation. ..
  39. Undergraduate Training Program in Chemical & Physical B*
    STEPHEN KRON; Fiscal Year: 2006
    ..abstract not available ..
  40. Hematopoietic Cell Survival by Protein Kinase CISK
    Zhou Songyang; Fiscal Year: 2007
    ..3. Investigate the physiological role of CISK in cell survival and development in mice, by studying how CISK affects hematopoietic cell development and survival using chimeric and knock-out mouse models. ..
  41. Molecular screening: MALDI-TOF scanning of peptides (RMI)
    STEPHEN KRON; Fiscal Year: 2008
    ..Compounds that appear to activate or inhibit one or more tyrosine kinases present in the cell extracts will be flagged for further analysis. ..
  42. Regulation of apoptosis in C. elegans
    JOEL ROTHMAN; Fiscal Year: 2008
    ..unreadable] [unreadable]..
  43. Telomere dysfunction, oxidative damage and breast cancer risk
    Jing Shen; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  44. Function of a membrane-localized nuclear receptor
    Joel H Rothman; Fiscal Year: 2010
    ..The elucidation of novel pathways through which NHRs function may advance our understanding of many developmental defects and the hormonal influences on normal human physiology and a variety of human pathologies. ..
  45. MECHANISMS OF METASTASIS IN EXPERIMENTAL PROSTATE CANCER
    TIMOTHY CHARLES THOMPSON; Fiscal Year: 2010
    ..abstract_text> ..
  46. The Functions of the Telomeric Protein TRF1
    Jan Karlseder; Fiscal Year: 2007
    ..Results from this study are expected to shed light onto the integrity of the telomeric complex, and therefore contribute to the understanding of aging and cancer development. ..
  47. RECOMBINATIONAL TELOMERE MAINTENANCE
    Michael J McEachern; Fiscal Year: 2010
    ....
  48. Undergraduate Training Program in Chemical & Physical B*
    STEPHEN KRON; Fiscal Year: 2007
    ..The University of Chicago PCBio program will be a major driver of curricular and scientific change that will impact undergraduates and faculty alike [unreadable] [unreadable]..
  49. Telomerase function and regulation in a new model system
    Neal Lue; Fiscal Year: 2007
    ..We anticipate that our findings will contribute to a detailed molecular understanding of telomerase function and regulation. ..
  50. Telomeric Function in Aging Syndromes
    Jan Karlseder; Fiscal Year: 2010
    ..Here we aim to investigate the role of telomere function in the syndrome, and to elucidate the connection between telomere replication, aging and cancer in Werner Syndrome. ..
  51. ANALYSIS OF TELOMERASE REVERSE TRANSCRIPTASE
    Neal F Lue; Fiscal Year: 2010
    ....
  52. Radiation-Induced G1 Checkpoint Arrest in Yeast
    Stephen J Kron; Fiscal Year: 2010
    ..These studies may define highly conserved molecular targets that will allow modulation of ionizing radiation effects on cancer and normal tissue. ..