cdc25 phosphatase

Summary

Summary: A tyrosine phosphatase required for progression of the cell cycle. It is required for p34(cdc2) kinase activation.

Top Publications

  1. pmc Regulation of Cdc2/cyclin B activation in Xenopus egg extracts via inhibitory phosphorylation of Cdc25C phosphatase by Ca(2+)/calmodulin-dependent protein [corrected] kinase II
    James R A Hutchins
    Biomedical Research Centre, Ninewells Hospital and Medical School, University of Dundee, Dundee DD1 9SY, Scotland, United Kingdom
    Mol Biol Cell 14:4003-14. 2003
  2. ncbi Cell cycle checkpoint proteins and cellular response to treatment by anticancer agents
    Giovanna Damia
    Laboratory of Molecular Pharmacology, Istituto di Ricerche Farmacologiche Mario Negri, Milan, Italy
    Cell Cycle 3:46-50. 2004
  3. pmc The rhodanese/Cdc25 phosphatase superfamily. Sequence-structure-function relations
    Domenico Bordo
    National Cancer Research Institute, c o Advanced Biotechnology Center, Genova, Italy
    EMBO Rep 3:741-6. 2002
  4. ncbi Inactivation of the Cdc25 phosphatase by the stress-activated Srk1 kinase in fission yeast
    Sandra López-Avilés
    Departament de Biologia Cellular, Institut de Investigacions Biomediques August Pi i Sunyer, Universitat de Barcelona, E 08036 Barcelona, Spain
    Mol Cell 17:49-59. 2005
  5. ncbi Cdc25b phosphatase is required for resumption of meiosis during oocyte maturation
    A Jeannine Lincoln
    National Cancer Institute FCRDC, Frederick, Maryland 21702, USA
    Nat Genet 30:446-9. 2002
  6. ncbi Phosphorylation of CDC25B by Aurora-A at the centrosome contributes to the G2-M transition
    Stéphanie Dutertre
    Groupe Cycle Cellulaire CNRS UMR6061 IFR97, Génomique Fonctionnelle et Santé, Universite de Rennes I, 35043 Rennes, France
    J Cell Sci 117:2523-31. 2004
  7. ncbi Phosphorylations of cyclin-dependent kinase 2 revisited using two-dimensional gel electrophoresis
    Katia Coulonval
    Institute of Interdisciplinary Research and Protein Chemistry Department, Faculte de Medecine, Universite Libre de Bruxelles, Campus Erasme, B 1070 Brussels, Belgium
    J Biol Chem 278:52052-60. 2003
  8. ncbi Chk1 regulates the S phase checkpoint by coupling the physiological turnover and ionizing radiation-induced accelerated proteolysis of Cdc25A
    Claus Storgaard Sørensen
    Danish Cancer Society, Institute of Cancer Biology, Strandboulevarden 49, DK 2100 Copenhagen Ø, Denmark
    Cancer Cell 3:247-58. 2003
  9. ncbi Dual phosphorylation controls Cdc25 phosphatases and mitotic entry
    Dmitry V Bulavin
    Gene Response Section, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Nat Cell Biol 5:545-51. 2003
  10. ncbi Cdc25 phosphatases and cancer
    K Kristjansdottir
    Departments of Biochemistry and Chemistry, Duke University Medical Center, LSRC Building, Room C125, Durham, North Carolina 27710, USA
    Chem Biol 11:1043-51. 2004

Detail Information

Publications194 found, 100 shown here

  1. pmc Regulation of Cdc2/cyclin B activation in Xenopus egg extracts via inhibitory phosphorylation of Cdc25C phosphatase by Ca(2+)/calmodulin-dependent protein [corrected] kinase II
    James R A Hutchins
    Biomedical Research Centre, Ninewells Hospital and Medical School, University of Dundee, Dundee DD1 9SY, Scotland, United Kingdom
    Mol Biol Cell 14:4003-14. 2003
    ..of Cdc2/cyclin B kinase and entry into mitosis requires dephosphorylation of inhibitory sites on Cdc2 by Cdc25 phosphatase. In vertebrates, Cdc25C is inhibited by phosphorylation at a single site targeted by the checkpoint kinases ..
  2. ncbi Cell cycle checkpoint proteins and cellular response to treatment by anticancer agents
    Giovanna Damia
    Laboratory of Molecular Pharmacology, Istituto di Ricerche Farmacologiche Mario Negri, Milan, Italy
    Cell Cycle 3:46-50. 2004
    ..Focus has also be given to the approaches taken to exploit the differential expression of these proteins between normal and tumor cells to improve the selectivity of treatment with anticancer agents...
  3. pmc The rhodanese/Cdc25 phosphatase superfamily. Sequence-structure-function relations
    Domenico Bordo
    National Cancer Research Institute, c o Advanced Biotechnology Center, Genova, Italy
    EMBO Rep 3:741-6. 2002
    ..investigations have shown that rhodanese domains are also structurally related to the catalytic subunit of Cdc25 phosphatase enzymes and that the two enzyme families are likely to share a common evolutionary origin...
  4. ncbi Inactivation of the Cdc25 phosphatase by the stress-activated Srk1 kinase in fission yeast
    Sandra López-Avilés
    Departament de Biologia Cellular, Institut de Investigacions Biomediques August Pi i Sunyer, Universitat de Barcelona, E 08036 Barcelona, Spain
    Mol Cell 17:49-59. 2005
    ..associates with the stress-activated p38/Sty1 MAP kinase, regulates the onset of mitosis by inhibiting the Cdc25 phosphatase. Srk1 is periodically active in G2, and its overexpression causes cell cycle arrest in late G2 phase, ..
  5. ncbi Cdc25b phosphatase is required for resumption of meiosis during oocyte maturation
    A Jeannine Lincoln
    National Cancer Institute FCRDC, Frederick, Maryland 21702, USA
    Nat Genet 30:446-9. 2002
    ..Cdc25b is therefore essential for meiotic resumption in female mice. Mice lacking Cdc25b provide the first genetic model for studying the mechanisms regulating prophase arrest in vertebrates...
  6. ncbi Phosphorylation of CDC25B by Aurora-A at the centrosome contributes to the G2-M transition
    Stéphanie Dutertre
    Groupe Cycle Cellulaire CNRS UMR6061 IFR97, Génomique Fonctionnelle et Santé, Universite de Rennes I, 35043 Rennes, France
    J Cell Sci 117:2523-31. 2004
    ..This phosphorylation might locally participate in the control of the onset of mitosis. These findings re-emphasise the role of the centrosome as a functional integrator of the pathways contributing to the triggering of mitosis...
  7. ncbi Phosphorylations of cyclin-dependent kinase 2 revisited using two-dimensional gel electrophoresis
    Katia Coulonval
    Institute of Interdisciplinary Research and Protein Chemistry Department, Faculte de Medecine, Universite Libre de Bruxelles, Campus Erasme, B 1070 Brussels, Belgium
    J Biol Chem 278:52052-60. 2003
    ....
  8. ncbi Chk1 regulates the S phase checkpoint by coupling the physiological turnover and ionizing radiation-induced accelerated proteolysis of Cdc25A
    Claus Storgaard Sørensen
    Danish Cancer Society, Institute of Cancer Biology, Strandboulevarden 49, DK 2100 Copenhagen Ø, Denmark
    Cancer Cell 3:247-58. 2003
    ..Our results provide evidence that the mammalian S phase checkpoint functions via amplification of physiologically operating, Chk1-dependent mechanisms...
  9. ncbi Dual phosphorylation controls Cdc25 phosphatases and mitotic entry
    Dmitry V Bulavin
    Gene Response Section, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Nat Cell Biol 5:545-51. 2003
    ..We propose that this pathway ensures that Cdc2 remains active once mitosis is initiated and is a key control mechanism for maintaining the proper order of cell-cycle transitions...
  10. ncbi Cdc25 phosphatases and cancer
    K Kristjansdottir
    Departments of Biochemistry and Chemistry, Duke University Medical Center, LSRC Building, Room C125, Durham, North Carolina 27710, USA
    Chem Biol 11:1043-51. 2004
    ....
  11. ncbi Proteomic screen finds pSer/pThr-binding domain localizing Plk1 to mitotic substrates
    Andrew E H Elia
    Center for Cancer Research, Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Science 299:1228-31. 2003
    ..This finding reveals how Plk1 can localize to specific sites within cells in response to Cdk phosphorylation at those sites and provides a structural mechanism for targeting the Plk1 kinase domain to its substrates...
  12. ncbi Cdc25A phosphatase: combinatorial phosphorylation, ubiquitylation and proteolysis
    Luca Busino
    European Institute of Oncology, 435 Via Ripamonti, Milan 20141, Italy
    Oncogene 23:2050-6. 2004
    ..Moreover, we discuss the role of phosphorylation at multiple sites in triggering ubiquitylation signals...
  13. pmc Regulating mammalian checkpoints through Cdc25 inactivation
    Maddalena Donzelli
    European Institute of Oncology, 435 Via Ripamonti, 20141 Milan, Italy
    EMBO Rep 4:671-7. 2003
    ..The evidence for links between checkpoint deregulation and oncogenesis is discussed...
  14. ncbi Degradation of Cdc25A by beta-TrCP during S phase and in response to DNA damage
    Luca Busino
    European Institute of Oncology, 435 Via Ripamonti, 20141 Milan, Italy
    Nature 426:87-91. 2003
    ..Our results show that beta-TrCP has a crucial role in mediating the response to DNA damage through Cdc25A degradation...
  15. ncbi Centrosome-associated Chk1 prevents premature activation of cyclin-B-Cdk1 kinase
    Alwin Krämer
    Danish Cancer Society, Institute of Cancer Biology, Department of Cell Cycle and Cancer, Strandboulevarden 49, DK 2100 Copenhagen Ø, Denmark
    Nat Cell Biol 6:884-91. 2004
    ....
  16. ncbi Many fingers on the mitotic trigger: post-translational regulation of the Cdc25C phosphatase
    James R A Hutchins
    Biomedical Research Centre, Ninewells Hospital and Medical School, University of Dundee, Dundee Scotland, UK
    Cell Cycle 3:41-5. 2004
    The mitotic inducer Cdc25 phosphatase controls the activation of Cdc2/cyclin B protein kinase and entry into mitosis in eukaryotic cells...
  17. ncbi Regulation of human Cdc25A stability by Serine 75 phosphorylation is not sufficient to activate a S phase checkpoint
    Anastasia Goloudina
    Gene Response Section, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Cell Cycle 2:473-8. 2003
    ..We propose that regulation of human Cdc25A stability by its phosphorylation at S75 may contribute to S phase checkpoint activation only in cooperation with other regulatory mechanisms...
  18. ncbi The cdc25 phosphatase is essential for the G2/M phase transition in the basidiomycete yeast Ustilago maydis
    Cecilia Sgarlata
    Department of Microbial Biotechnology, Centro Nacional de Biotecnologia CSIC, Campus de Cantoblanco UAM, 28049 Madrid, Spain
    Mol Microbiol 58:1482-96. 2005
    ..maydis Cdc25 counteracts the Wee1-mediated inhibitory phosphorylation of Cdk1-Clb2 complex. Our results supports a model in which inhibitory phosphorylation of Cdk1 is a primary mechanism operating at G2/M transition in this fungus...
  19. ncbi Assaying Cdc25 phosphatase activity
    Ingo Hassepass
    Cell Cycle Control and Carcinogenesis, German Cancer Research Center DKFZ, Heidelberg
    Methods Mol Biol 281:153-62. 2004
    ..To analyze endogenous Cdc25 phosphatase activities of immunoprecipitates from total cellular extracts, the physiological substrate Cdk1/cyclin B1 is ..
  20. ncbi Cdc25A localisation and shuttling: characterisation of sequences mediating nuclear export and import
    Helena Källström
    Department of Cell and Molecular Biology, Karolinska Institutet, 171 77 Stockholm, Sweden
    Exp Cell Res 303:89-100. 2005
    ..Our results imply that the Cdc25A phosphatase may interact with substrates and regulators both in the nucleus and the cytoplasm...
  21. pmc Dual mode of degradation of Cdc25 A phosphatase
    Maddalena Donzelli
    European Institute of Oncology, 435 Via Ripamonti, I 20141 Milan, Italy
    EMBO J 21:4875-84. 2002
    ..We propose that a dual mechanism of regulated degradation allows for fine tuning of Cdc25 A abundance in response to cell environment...
  22. pmc Plk1 promotes nuclear translocation of human Cdc25C during prophase
    Fumiko Toyoshima-Morimoto
    Department of Cell and Developmental Biology, Graduate School of Biostudies, Kyoto University, Sakyo ku, Kyoto 606 8502, Japan
    EMBO Rep 3:341-8. 2002
    ..These results suggest that Plk1 phosphorylates Cdc25C on Ser198 and regulates nuclear translocation of Cdc25C during prophase...
  23. ncbi What if higher plants lack a CDC25 phosphatase?
    Véronique Boudolf
    Department of Plant Systems Biology, Flanders Interuniversity Institute for Biotechnology VIB, Ghent University, Technologiepark 927, B 9052 Gent, Belgium
    Trends Plant Sci 11:474-9. 2006
    ..Plants possess a unique class of CDKs, designated B-type CDKs, but seem to lack a functional CDC25 phosphatase, which is a crucial activator of the onset of mitosis in non-plant species...
  24. ncbi What's new on CDC25 phosphatase inhibitors
    Marie Odile Contour-Galcera
    Ipsen Research Laboratories, Institut Henri Beaufour, 5, Avenue du Canada, F 91966 Les Ulis Cédex, France
    Pharmacol Ther 115:1-12. 2007
    ..These promising results confirm the interest of the inhibition of CDC25 phosphatases as an anticancer therapeutic strategy...
  25. ncbi NAD(P)H:quinone oxidoreductase-1-dependent and -independent cytotoxicity of potent quinone Cdc25 phosphatase inhibitors
    Yusheng Han
    Department of Pharmacology, Hillman Cancer Center, University of Pittsburgh Cancer Institute, 5117 Centre Avenue, Pittsburgh, PA 15213, USA
    J Pharmacol Exp Ther 309:64-70. 2004
    ..Thus, NQO1 detoxified Cpd 5, probably by reducing it to a less active hydroquinone, whereas NSC 95397- and NSC 663284-generated cytotoxicity was unaffected by NQO1...
  26. pmc Regulation of G(2)/M events by Cdc25A through phosphorylation-dependent modulation of its stability
    Niels Mailand
    Danish Cancer Society, Institute of Cancer Biology, Strandboulevarden 49, DK 2100 Copenhagen Ø, Denmark
    EMBO J 21:5911-20. 2002
    ..Thus, phosphorylation-mediated switches among three differentially stable forms ensure distinct thresholds, and thereby distinct roles for Cdc25A in multiple cell cycle transitions and checkpoints...
  27. pmc SCFbeta-TRCP links Chk1 signaling to degradation of the Cdc25A protein phosphatase
    Jianping Jin
    Department of Pathology, Howard Hughes Medical Institute, Harvard Medical School, Boston, MA 02115, USA
    Genes Dev 17:3062-74. 2003
    ..These data indicate that Cdc25A turnover is more complex than previously appreciated and suggest roles for an additional kinase(s) in Chk1-dependent Cdc25A turnover...
  28. ncbi Phosphorylation at serine 75 is required for UV-mediated degradation of human Cdc25A phosphatase at the S-phase checkpoint
    Ingo Hassepass
    German Cancer Research Center DKFZ, Department Applied Tumorvirology, Im Neuenheimer Feld 242, 69120 Heidelberg, Germany
    J Biol Chem 278:29824-9. 2003
    ..Taken together, these data strongly suggest that phosphorylation of Cdc25A on Ser-75 by Chk1 and its subsequent degradation is required to delay cell cycle progression in response to UV-induced DNA lesions...
  29. pmc Cyclin A/Cdk2 complexes regulate activation of Cdk1 and Cdc25 phosphatases in human cells
    Jayashree Mitra
    Departments of Medicine and Genetics and Cancer Center, University of Pennsylvania, Philadelphia, PA 19104 6140, USA
    Oncogene 23:3361-7. 2004
    ..These results suggest that cyclin A/Cdk2 complexes are key regulators of Cdc25 and Cdk1 activation in human cells. This pathway appears to be commonly deregulated in cancer...
  30. pmc Functional cdc25C dual-specificity phosphatase is required for S-phase entry in human cells
    Patric Turowski
    Cell Biology Unit, Institut de Genetique Humaine, Centre National de la Recherche Scientifique Unité Mixte de Recherche 1142, F 34396 Montpellier, France
    Mol Biol Cell 14:2984-98. 2003
    ..Taken together, these results show that cdc25C not only plays a role at the G2/M transition but also in the modulation of DNA replication where its function is distinct from that of cdc25A...
  31. ncbi Characterisation of Cdc25B localisation and nuclear export during the cell cycle and in response to stress
    Arne Lindqvist
    Department of Cell and Molecular Biology, Karolinska Institutet, S 171 77 Stockholm, Sweden
    J Cell Sci 117:4979-90. 2004
    ....
  32. ncbi Catalytic and chemical competence of regulation of cdc25 phosphatase by oxidation/reduction
    Jungsan Sohn
    Department of Biochemistry, Duke University Medical Center, Durham, North Carolina 27710, USA
    Biochemistry 42:10060-70. 2003
    ..Thus, the chemistry and kinetics of the active-site cysteines of the Cdc25's support a physiological role for reversible redox-mediated regulation of the Cdc25's, important regulators of the eukaryotic cell cycle...
  33. pmc Chk1 is activated transiently and targets Cdc25A for degradation at the Xenopus midblastula transition
    Ken Shimuta
    Department of Biology, Graduate School of Sciences, Kyushu University, Hakozaki 6 10 1, Fukuoka 812 8581, Japan
    EMBO J 21:3694-703. 2002
    ..Thus, the physiological replication checkpoint is activated transiently at the MBT by developmental cues, and activated Chk1, only together with an unknown kinase, targets Cdc25A for degradation to ensure later development...
  34. ncbi Polo-like kinase-1 controls recovery from a G2 DNA damage-induced arrest in mammalian cells
    Marcel A T M van Vugt
    Division of Molecular Biology, H8, Netherlands Cancer Institute, Plesmanlaan 121, 1066CX Amsterdam, The Netherlands
    Mol Cell 15:799-811. 2004
    ..Thus, our data show that the cell cycle machinery is reset in response to DNA damage and that cells become critically dependent on Plk1-mediated degradation of Wee1 for their recovery...
  35. ncbi The Arabidopsis 14-3-3 protein, GF14omega, binds to the Schizosaccharomyces pombe Cdc25 phosphatase and rescues checkpoint defects in the rad24- mutant
    David A Sorrell
    Hannah Research Institute, KA6 5HL, Ayr, UK
    Planta 218:50-7. 2003
    ..The data are consistent with the hypothesis that interaction between Spcdc25 and the plant cell cycle occurs at the level of a 14-3-3 protein with distinct checkpoint properties...
  36. pmc Remote hot spots mediate protein substrate recognition for the Cdc25 phosphatase
    J Sohn
    Departments of Biochemistry and Chemistry, Duke University Medical Center, Durham, NC 27710, USA
    Proc Natl Acad Sci U S A 101:16437-41. 2004
    ....
  37. ncbi Hierarchical order of phosphorylation events commits Cdc25A to betaTrCP-dependent degradation
    Maddalena Donzelli
    European Institute of Oncology, Milan, Italy
    Cell Cycle 3:469-71. 2004
    ..According to our model, phosphorylation at Ser 76 is a "priming" step required for Ser 82 phosphorylation, which in turn allows recruitment of Cdc25A by betaTrCP and subsequent betaTrCP-dependent degradation...
  38. ncbi A p21(waf1)-independent pathway for inhibitory phosphorylation of cyclin-dependent kinase p34(cdc2) and concomitant G(2)/M arrest by the chemopreventive flavonoid apigenin
    Maralee McVean
    Department of Pathology and Laboratory Medicine, University of Kansas Medical Center, Kansas City, Kansas 66160, USA
    Mol Carcinog 33:36-43. 2002
    ..These results supported the conclusion that G(2)/M arrest induced by apigenin was accompanied by inhibition of the p34(cdc2) cyclin-dependent kinase protein level and activity in a p21(waf1)-independent manner...
  39. ncbi Resveratrol causes Cdc2-tyr15 phosphorylation via ATM/ATR-Chk1/2-Cdc25C pathway as a central mechanism for S phase arrest in human ovarian carcinoma Ovcar-3 cells
    Alpna Tyagi
    Department of Pharmaceutical Sciences, School of Pharmacy, University of Colorado Cancer Center, Denver, CO 80262, USA
    Carcinogenesis 26:1978-87. 2005
    ....
  40. ncbi Modulation of cell-cycle regulatory signaling network by 2-methoxyestradiol in prostate cancer cells is mediated through multiple signal transduction pathways
    Gibanananda Ray
    Cancer Research Unit, Veterans Affairs Medical Center, Kansas City, Missouri, USA
    Biochemistry 45:3703-13. 2006
    ....
  41. ncbi Checkpoint kinase 1-mediated phosphorylation of Cdc25C and bad proteins are involved in antitumor effects of loratadine-induced G2/M phase cell-cycle arrest and apoptosis
    Jinn Shiun Chen
    Department of Surgery, Division of Colon and Rectal Surgery, Chang Gung Memorial Hospital, Linkou, Taiwan
    Mol Carcinog 45:461-78. 2006
    ....
  42. ncbi CDC25B phosphorylation by Aurora-A occurs at the G2/M transition and is inhibited by DNA damage
    Martine Cazales
    LBCMCP CNRS UMR5088 IFR109, Institut d Exploration Fonctionnelle des Génomes, Universite Paul Sabatier, Toulouse, France
    Cell Cycle 4:1233-8. 2005
    ..These results strongly suggest that Aurora-A-mediated phosphorylation of CDC25B at the centrosome is an important step contributing to the earliest events inducing mitosis, upstream of CDK1-cyclin B1 activation...
  43. pmc DNA damage during the spindle-assembly checkpoint degrades CDC25A, inhibits cyclin-CDC2 complexes, and reverses cells to interphase
    Jeremy P H Chow
    Department of Biochemistry, Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong
    Mol Biol Cell 14:3989-4002. 2003
    ..Hence, although spindle disruption and DNA damage provide conflicting signals to regulate CDC2, the negative regulation by the DNA damage checkpoint could overcome the positive regulation by the spindle-assembly checkpoint...
  44. ncbi ATR, Claspin and the Rad9-Rad1-Hus1 complex regulate Chk1 and Cdc25A in the absence of DNA damage
    Claus Storgaard Sørensen
    Danish Cancer Society, Institute of Cancer Biology, Copenhagen, Denmark
    Cell Cycle 3:941-5. 2004
    ..DNA damage further activates and switches this pathway from its constitutively operating "surveillance mode" compatible with DNA replication into an "emergency" checkpoint response...
  45. ncbi Elevated phosphorylation of Chk1 and decreased phosphorylation of Chk2 are associated with abrogation of G2/M checkpoint control during transformation of Syrian hamster embryo (SHE) cells by Malachite green
    Hima Ashra
    Chemical Carcinogenesis Group, Advanced Centre for Treatment, Research and Education in Cancer ACTREC, Tata Memorial Centre, Kharghar, Navi Mumbai 410208, India
    Cancer Lett 237:188-98. 2006
    ....
  46. ncbi S-phase arrest by reactive nitrogen species is bypassed by okadaic acid, an inhibitor of protein phosphatases PP1/PP2A
    Priya Ranjan
    Department of Pathology and Vermont Cancer Center, University of Vermont College of Medicine, Burlington, VT 05405, USA
    Free Radic Biol Med 40:247-59. 2006
    ..Our studies suggest that RNS may induce S-phase arrest through mechanisms that differ from those elicited by classical DNA-damaging agents...
  47. ncbi A functional role for p38 MAPK in modulating mitotic transit in the absence of stress
    Hyukjin Cha
    Department of Genetics and Complex Diseases, School of Public Health, and John B Little Center for the Radiation Sciences and Environmental Health, Harvard University, Boston, Massachusetts 02115, USA
    J Biol Chem 282:22984-92. 2007
    ..Taken together, our findings suggest that p38 regulates the timing of mitotic entry via modulation of Cdc25B activity under normal nonstress conditions...
  48. ncbi The first green lineage cdc25 dual-specificity phosphatase
    B Khadaroo
    Universite Paris VI, Laboratoire Arago, Modèles en Biologie cellulaire et évolutive, Banyuls sur Mer, France
    Cell Cycle 3:513-8. 2004
    ..tauri Cdc25. We propose that there has been coevolution of the regulatory proteins involved in the control of M-phase entry in the metazoan, yeast and green lineages...
  49. ncbi Regulation of mitotic entry by microcephalin and its overlap with ATR signalling
    Gemma K Alderton
    Genome Damage and Stability Centre, University of Sussex, East Sussex, BN1 9RQ, UK
    Nat Cell Biol 8:725-33. 2006
    ..Thus, MCPH1 also has an ATR-independent role in maintaining inhibitory Cdk1 phosphorylation, which prevents premature entry into mitosis...
  50. ncbi Phosphorylation of Plk1 at S137 and T210 is inhibited in response to DNA damage
    Lyuben Tsvetkov
    Department of Pathology, School of Medicine, Yale University, New Haven, Connecticut 06510, USA
    Cell Cycle 4:166-71. 2005
    ..These data suggest a participation of DNA damage checkpoints in regulation of the signaling pathways upstream of Plk1...
  51. pmc p53-deficient cells rely on ATM- and ATR-mediated checkpoint signaling through the p38MAPK/MK2 pathway for survival after DNA damage
    H Christian Reinhardt
    Center for Cancer Research, Massachusetts Institute of Technology, 77 Massachusetts Avenue, E18 580, Cambridge, MA 02139, USA
    Cancer Cell 11:175-89. 2007
    ..We show that the Chk1 inhibitor UCN-01 also potently inhibits MK2, suggesting that its clinical efficacy results from the simultaneous disruption of two critical checkpoint pathways in p53-defective cells...
  52. ncbi p14ARF induces G2 cell cycle arrest in p53- and p21-deficient cells by down-regulating p34cdc2 kinase activity
    Guillaume Normand
    Department of Hematology, Oncology and Tumor Immunology, University Medical Center Charite, Campus Berlin Buch, D 13125 Berlin Buch, Germany
    J Biol Chem 280:7118-30. 2005
    ..This may represent an important fail-safe mechanism by which p14(ARF) protects p53/p21-deficient cells from unrestrained proliferation...
  53. pmc Rereplication by depletion of geminin is seen regardless of p53 status and activates a G2/M checkpoint
    Wenge Zhu
    Department of Biochemistry and Molecular Genetics, University of Virginia School of Medicine, Charlottesville, VA 22908, USA
    Mol Cell Biol 24:7140-50. 2004
    ..From these results, we conclude that geminin is required for suppressing overreplication in human cells and that a G(2)/M checkpoint restricts the proliferation of cells with overreplicated DNA...
  54. ncbi Irradiation-induced G2/M checkpoint response requires ERK1/2 activation
    Y Yan
    Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, Omaha, NE 68198 6805, USA
    Oncogene 26:4689-98. 2007
    ..These results implicate IR-induced ERK1/2 activation as an important regulator of G2/M checkpoint response to IR in MCF-7 cells...
  55. ncbi Escherichia coli cyclomodulin Cif induces G2 arrest of the host cell cycle without activation of the DNA-damage checkpoint-signalling pathway
    Frédéric Taïeb
    UMR 1225, IHAP, INRA ENVT, 23 Chemin des Capelles, BP 87614, 31000 Toulouse, France
    Cell Microbiol 8:1910-21. 2006
    ..Hence, Cif activates a DNA damage-independent signalling pathway that leads to inhibition of the G(2)/M transition...
  56. ncbi Gallic acid causes inactivating phosphorylation of cdc25A/cdc25C-cdc2 via ATM-Chk2 activation, leading to cell cycle arrest, and induces apoptosis in human prostate carcinoma DU145 cells
    Chapla Agarwal
    Department of Pharmaceutical Sciences, School of Pharmacy, University of Colorado Health Sciences Center, 4200 East Ninth Avenue, Box C238, Denver, CO 80262, USA
    Mol Cancer Ther 5:3294-302. 2006
    ....
  57. pmc Apigenin inhibits pancreatic cancer cell proliferation through G2/M cell cycle arrest
    Michael B Ujiki
    Department of Surgery and Robert H, Lurie Comprehensive Cancer Center, Feinberg School of Medicine, Northwestern University, Chicago, Illinois, USA
    Mol Cancer 5:76. 2006
    ..Apigenin, a naturally occurring flavonoid, has been shown to inhibit growth in some cancer cell lines but has not been studied in pancreatic cancer. We hypothesized that apigenin would inhibit pancreatic cancer cell growth in vitro...
  58. ncbi Enhanced 7-ethyl-10-hydroxycamptothecin (SN-38) lethality by methylselenocysteine is associated with Chk2 phosphorylation at threonine-68 and down-regulation of Cdc6 expression
    Ming biao Yin
    Department of Pharmacology and Therapeutics, Grace Cancer Drug Center, Roswell Park Cancer Institute, Elm and Carlton Streets, Buffalo, NY 14263, USA
    Mol Pharmacol 66:153-60. 2004
    ....
  59. ncbi SHP-2 phosphatase regulates DNA damage-induced apoptosis and G2/M arrest in catalytically dependent and independent manners, respectively
    Liangping Yuan
    Department of Medicine, Division of Hematology Oncology, Case Comprehensive Cancer Center, Case Western Reserve University, Cleveland, Ohio 44106, USA
    J Biol Chem 280:42701-6. 2005
    ..Collectively, these studies have further defined the mechanisms by which SHP-2 phosphatase regulates DNA damage responses...
  60. ncbi Sulforaphane-induced G2/M phase cell cycle arrest involves checkpoint kinase 2-mediated phosphorylation of cell division cycle 25C
    Shivendra V Singh
    Department of Pharmacology and University of Pittsburgh Cancer Institute, Pittsburgh, PA 15213, USA
    J Biol Chem 279:25813-22. 2004
    ..Activation of Chk2 in response to DNA damage is well documented, but the present study is the first published report to link Chk2 activation to cell cycle arrest by an isothiocyanate...
  61. ncbi HCLK2 is essential for the mammalian S-phase checkpoint and impacts on Chk1 stability
    Spencer J Collis
    DNA Damage Response Laboratory, Cancer Research UK, The London Research Institute, Clare Hall Laboratories, South Mimms, EN6 3LD, UK
    Nat Cell Biol 9:391-401. 2007
    ..We conclude that HCLK2 promotes activation of the S-phase checkpoint and downstream repair responses by preventing unscheduled Chk1 degradation by the proteasome...
  62. ncbi Relationship between HPV typing and the status of G2 cell cycle regulators in cervical neoplasia
    Yasunori Hashiguchi
    Department of Obstetrics and Gynecology, Osaka City General Hospital, 2 13 22 Miyako jimahondori Miyakojima, Osaka 534 0021, Japan
    Oncol Rep 12:587-91. 2004
    ..9%) (p=0.0042). However, the relationship between HPV typing and ATM, chk2, CDC25C and cyclinB1 expression was not significant. Cdc2 is implicated in cervical carcinogenesis and may be related to p53 inactivation by HPV...
  63. ncbi Modelling the fission yeast cell cycle
    Akos Sveiczer
    Department of Agricultural Chemical Technology at Budapest University of Technology and Economics, Hungary
    Brief Funct Genomic Proteomic 2:298-307. 2004
    ..It is proposed that the key physiological variable driving progression through the cell cycle during balanced growth and division is the mass/DNA ratio, rather than the mass/nucleus ratio...
  64. pmc Specialized roles of the two mitotic cyclins in somatic cells: cyclin A as an activator of M phase-promoting factor
    Tsz Kan Fung
    Department of Biochemistry, Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong, China
    Mol Biol Cell 18:1861-73. 2007
    ..These observations show that cyclin A cannot form MPF independent of cyclin B and underscore a critical role of cyclin A as a trigger for MPF activation...
  65. ncbi Chk1-dependent regulation of Cdc25B functions to coordinate mitotic events
    Harald Loffler
    Clinical Cooperation Unit for Molecular Hematology Oncology, German Cancer Research Center DKFZ
    Cell Cycle 5:2543-7. 2006
    ..Analogous to Chk1 inhibition, the 'paraspindle' phenotype was induced by overexpression of Cdc25B but not Cdc25A. Our results suggest that Chk1 functions to coordinate mitotic events through regulation of Cdc25B...
  66. ncbi BRCA1-mediated G2/M cell cycle arrest requires ERK1/2 kinase activation
    Ying Yan
    Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, 986805 Nebraska Medical Center, Omaha, NE 68198 6805, USA
    Oncogene 24:3285-96. 2005
    ..These results implicate a critical role of ERK1/2 signaling in the regulation of BRCA1 function on controlling the G2/M checkpoint responses...
  67. pmc p21-Mediated nuclear retention of cyclin B1-Cdk1 in response to genotoxic stress
    Fabienne Baus Charrier-Savournin
    CRBM Centre National de la Recherche Scientifique FRE 2593, 34293 Montpellier, France
    Mol Biol Cell 15:3965-76. 2004
    ..This, with subsequent degradation of mitotic cyclins, further contributes to the establishment of a permanent G2 arrest...
  68. ncbi Expression of wee1 and its related cell cycle components in mouse early stage follicles
    Chang Eun Park
    Infertility Medical Center, CHA General Hospital, Seoul, Korea
    Cells Tissues Organs 177:221-8. 2004
    ..Expression of all these proteins in the granulosa cells of growing follicles may regulate granulosa cell mitosis concurrently with the growth of oocytes and follicles...
  69. ncbi DNA damage-induced G2/M checkpoint in SV40 large T antigen-immortalized embryonic fibroblast cells requires SHP-2 tyrosine phosphatase
    Liangping Yuan
    Department of Hematopoiesis, Jerome H Holland Laboratory for the Biomedical Sciences, American Red Cross, Rockville, Maryland 20855, USA
    J Biol Chem 278:42812-20. 2003
    ..These results together provide the first evidence that SHP-2 tyrosine phosphatase enhances the DNA damage G2/M checkpoint in SV40 large T antigen immortalized murine embryonic fibroblast cells...
  70. pmc Arsenite-induced Cdc25C degradation is through the KEN-box and ubiquitin-proteasome pathway
    Fei Chen
    Health Effects Laboratory Division, National Institute for Occupational Safety and Health, 1095 Willowdale Road, Morgantown, WV 26505, USA
    Proc Natl Acad Sci U S A 99:1990-5. 2002
    ....
  71. ncbi HIV-1 VPR modulates cell cycle G2/M transition through an alternative cellular mechanism other than the classic mitotic checkpoints
    Robert T Elder
    Children s Memorial Institute for Education and Research, Northwestern University Medical School, 2430 N Halsted Street, MB218, Chicago, Illinois 60614, USA
    Front Biosci 7:d349-57. 2002
    ..A number of cellular proteins which may be involved in this new cellular pathway(s) have been identified and are discussed...
  72. ncbi Caffeine induces TP53-independent G(1)-phase arrest and apoptosis in human lung tumor cells in a dose-dependent manner
    Wenqing Qi
    Department of Radiation Oncology, The University of Arizona, 1501 N Campbell Avenue, P O Box 245024, Tucson, Arizona 85724, USA
    Radiat Res 157:166-74. 2002
    ..These data indicate that the effect of caffeine on cell cycle checkpoints and apoptosis is dependent on dose and that caffeine acts through differential regulation of cyclin-dependent kinase activity...
  73. ncbi Abrogation of the S phase DNA damage checkpoint results in S phase progression or premature mitosis depending on the concentration of 7-hydroxystaurosporine and the kinetics of Cdc25C activation
    Ethan A Kohn
    Department of Pharmacology and Toxicology, Dartmouth Medical School, Hanover, New Hampshire 03755, USA
    J Biol Chem 277:26553-64. 2002
    ..Hence, UCN-01 has multiple effects depending on concentration and cell phenotype that must be considered when investigating mechanisms of checkpoint regulation...
  74. pmc Effect of CD26/dipeptidyl peptidase IV on Jurkat sensitivity to G2/M arrest induced by topoisomerase II inhibitors
    U Aytac
    Department of Lymphoma Myeloma, MD Anderson Cancer Center, Houston, TX 77030, USA
    Br J Cancer 88:455-62. 2003
    ....
  75. ncbi MDC1 is required for the intra-S-phase DNA damage checkpoint
    Michal Goldberg
    The Wellcome Trust Cancer Research UK Institute of Cancer and Developmental Biology and Department of Zoology, University of Cambridge, Cambridge CB2 1QR, UK
    Nature 421:952-6. 2003
    ..These findings reveal that MDC1-mediated focus formation by the MRE11 complex at sites of DNA damage is crucial for the efficient activation of the intra-S-phase checkpoint...
  76. ncbi Inhibition of cell growth by conditional expression of kpm, a human homologue of Drosophila warts/lats tumor suppressor
    Yasuhiko Kamikubo
    Department of Hematology and Oncology, Graduate School of Medicine, Kyoto University, Kyoto 606 8507, Japan
    J Biol Chem 278:17609-14. 2003
    ..Taken together, these results indicate that kpm negatively regulates cell growth by inducing G(2)/M arrest and apoptotic cell death through its kinase activity...
  77. ncbi Genistein arrests hepatoma cells at G2/M phase: involvement of ATM activation and upregulation of p21waf1/cip1 and Wee1
    Kee Lung Chang
    Department of Biochemistry, Kaohsiung Medical University, Kaohsiung 807, Taiwan, ROC
    Biochem Pharmacol 67:717-26. 2004
    ....
  78. ncbi Role of reactive oxygen species and MAPKs in vanadate-induced G(2)/M phase arrest
    Zhuo Zhang
    Pathology and Physiology Research Branch, National Institute for Occupational Safety and Health, Morgantown, WV 26505, USA
    Free Radic Biol Med 34:1333-42. 2003
    ..Specific ROS affect different MAPK family members and cell growth regulatory proteins with different potencies...
  79. ncbi Monensin-mediated growth inhibition in NCI-H929 myeloma cells via cell cycle arrest and apoptosis
    Woo Hyun Park
    Cancer Research Institute, Seoul National University College of Medicine, Seoul, Korea
    Int J Oncol 23:197-204. 2003
    ..Taken together, these results have demonstrated for the first time that monensin potently inhibited the proliferation of human myeloma cell lines, especially NCI-H929 cells, via cell cycle arrest in association with p21 and apoptosis...
  80. ncbi Cdc2-cyclin B triggers H3 kinase activation of Aurora-A in Xenopus oocytes
    Gilliane Maton
    Laboratoire de Biologie du Developpement, Unite Mixte de Recherche CNRS 7622, Universite Pierre et Marie Curie, Boite 24, 4 place Jussieu, Paris 75252 Cedex 05, France
    J Biol Chem 278:21439-49. 2003
    ..Therefore, Aurora-A is under the control of Cdc2 in the Xenopus oocyte and could be involved in meiotic spindle establishment...
  81. ncbi Isoobtusilactone A induces cell cycle arrest and apoptosis through reactive oxygen species/apoptosis signal-regulating kinase 1 signaling pathway in human breast cancer cells
    Po Lin Kuo
    Cell Biology Laboratory, Department of Biotechnology, Chia Nan University of Pharmacy and Science, Tainan, Taiwan
    Cancer Res 67:7406-20. 2007
    ..Moreover, blocking ASK1 by small interfering RNA inhibition completely suppressed IOA-induced apoptosis. Taken together, these results imply a critical role for ROS and ASK1 in IOA's anticancer activity...
  82. ncbi SAPK/JNK regulates cdc2/cyclin B kinase through phosphorylation and inhibition of cdc25c
    Valerie L Goss
    Institute of Pathology, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH 44106, USA
    Cell Signal 15:709-18. 2003
    ..We conclude that SAPK regulates cdc2/cyclin B kinase following stress events by a novel mechanism involving inhibitory phosphorylation of the cdc2-activating phosphatase cdc25c on S168...
  83. ncbi Requirement for phosphatidylinositol-3 kinase activity during progression through S-phase and entry into mitosis
    Surabhi Dangi
    Department of Pharmaceutical Sciences, University of Maryland School of Pharmacy, Baltimore, MD 21201, USA
    Cell Signal 15:667-75. 2003
    ..Thus, similar to its role in promoting cell survival and cell cycle transitions from G1 to S phase, PI3K activity appears to promote entry into mitosis and protect against cell death during S- and G2-phase progression...
  84. ncbi Unmasking the S-phase-promoting potential of cyclin B1
    Jonathan D Moore
    Cancer Research UK London Research Institute, Clare Hall Laboratories, Blanche Lane, South Mimms, Herts EN6 3LD, UK
    Science 300:987-90. 2003
    ..Subcellular localization of vertebrate CDKs and the control of their activity are thus critical factors for determining their specificity...
  85. ncbi Allyl isothiocyanate, a constituent of cruciferous vegetables, inhibits proliferation of human prostate cancer cells by causing G2/M arrest and inducing apoptosis
    Dong Xiao
    Department of Pharmacology, University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine, PA 15213, USA
    Carcinogenesis 24:891-7. 2003
    ..In conclusion, it seems reasonable to hypothesize that AITC, and possibly other ITCs, may find use in the treatment of human prostate cancers...
  86. ncbi Alteration of G2 cell cycle regulators occurs during carcinogenesis of the endometrium
    Hiroshi Tsuda
    Department of Obstetrics and Gynecology, Osaka City General Hospital, Osaka, Japan
    Oncology 65:159-66. 2003
    ..In this study, we examined the alteration of the G2 pathway in endometrial hyperplasia (EH) and endometrioid-type endometrial cancer (EC), and analyzed the relationship between the G2 pathway status and the p53 pathway status...
  87. ncbi Involvement of the dehydroleucodine alpha-methylene-gamma-lactone function in GVBD inhibition in Bufo arenarum oocytes
    G Sanchez Toranzo
    Departamento de Biologia del Desarrollo, Instituto Superior de Investigaciones Biológicas INSIBIO y Universidad Nacional de Tucumán UNT, San Miguel de Tucuman, Argentina
    Zygote 18:41-9. 2010
    ..pathways leading to MPF activation suggests that the Myt1 kinase could be a target of these lactones, while cdc25 phosphatase would not be affected...
  88. ncbi Effect of dehydroleucodine on meiosis reinitiation in Bufo arenarum denuded oocytes
    G Sanchez Toranzo
    Departamento de Biologia del Desarrollo, Chacabuco, San Miguel de Tucuman, Argentina
    Zygote 15:183-7. 2007
    ..of Thr-14/Tyr-15 is necessary for the start of MPF activation and it is induced by the activation of cdc25 phosphatase. Late, to complete the activation, a small amount of active MPF induces an auto-amplification loop of MPF ..
  89. ncbi A model of Cdc25 phosphatase catalytic domain and Cdk-interaction surface based on the presence of a rhodanese homology domain
    K Hofmann
    Bioinformatics Group, Swiss Institute for Experimental Cancer Research, Chemin des Boveresses 155, Epalinges, CH 1066, Switzerland
    J Mol Biol 282:195-208. 1998
    Mammalian Cdc25 phosphatase is responsible for the dephosphorylation of Cdc2 and other cyclin-dependent kinases at Thr14 and Tyr15, thus activating the kinase and allowing cell cycle progression...
  90. pmc Adventitious arsenate reductase activity of the catalytic domain of the human Cdc25B and Cdc25C phosphatases
    Hiranmoy Bhattacharjee
    Department of Cellular Biology and Pharmacology, Florida International University, Herbert Wertheim College of Medicine, Miami, Florida 33199, USA
    Biochemistry 49:802-9. 2010
    ..eukaryotic enzymes that function as arsenate reductases are homologues of the catalytic domain of the human Cdc25 phosphatase. For example, the Leishmania major enzyme LmACR2 is both a phosphatase and an arsenate reductase, and its ..
  91. pmc Dynamic control of cell cycle and growth coupling by ecdysone, EGFR, and PI3K signaling in Drosophila histoblasts
    Nikolay Ninov
    Instituto de Biologia Molecular de Barcelona, Consejo Superior de Investigaciones Cientificas, Barcelona, Spain
    PLoS Biol 7:e1000079. 2009
    ..stages, enter a proliferative stage in the pupal period that is initiated by ecdysone-dependent string/Cdc25 phosphatase transcription...
  92. ncbi Terminal mitoses require negative regulation of Fzr/Cdh1 by Cyclin A, preventing premature degradation of mitotic cyclins and String/Cdc25
    Achim Reber
    BZMB, Department of Genetics, University of Bayreuth, 95440 Bayreuth, Germany
    Development 133:3201-11. 2006
    ..results in the premature degradation of the Cdk1 activators Cyclin B and Cyclin B3, and apparently of String/Cdc25 phosphatase as well. Without these activators, entry into terminal mitoses is not possible...
  93. ncbi Cell division genes promote asymmetric interaction between Numb and Notch in the Drosophila CNS
    P Wai
    Department of Cell Biology, Emory University School of Medicine, Atlanta, GA 30322, USA
    Development 126:2759-70. 1999
    ..mutations in N and nb, cell division mutants cyclinA (cycA), regulator of cyclin A1 (rca1) and string/cdc25 phosphatase (stg), and the microtubule destabilizing agent, nocodazole, to investigate this issue...
  94. pmc Regulation of Mih1/Cdc25 by protein phosphatase 2A and casein kinase 1
    Gayatri Pal
    Department of Molecular, Cell and Developmental Biology, University of California, Santa Cruz, Santa Cruz, CA 95064, USA
    J Cell Biol 180:931-45. 2008
    The Cdc25 phosphatase promotes entry into mitosis by removing cyclin-dependent kinase 1 (Cdk1) inhibitory phosphorylation...
  95. ncbi Activation of both Mos and Cdc25 is required for G2-M transition in perch oocyte
    Anamika Priyadarshini
    Department of Zoology, School of Life Science, Visva Bharati A Central University, Santiniketan, India
    Mol Reprod Dev 76:289-300. 2009
    ..on the withdrawal of Cdk1 inhibition by Wee1/Myt1 kinase on the one hand and the activation of Cdk1 by Cdc25 phosphatase on the other...
  96. ncbi Tobacco cells transformed with the fission yeast Spcdc25 mitotic inducer display growth and morphological characteristics as well as starch and sugar status evocable by cytokinin application
    Petra Suchomelová-Masková
    Department of Plant Physiology, Faculty of Science, Charles University in Prague, Vinicna 5, 128 44 Prague 2, Czech Republic
    Plant Physiol Biochem 46:673-84. 2008
    ..an elusive issue as doubts persist about activatory dephosphorylation--in other eukaryotes provided by CDC25 phosphatase and serving as a final all-or-nothing mitosis regulator...
  97. pmc cdc25 is a nuclear protein expressed constitutively throughout the cell cycle in nontransformed mammalian cells
    F Girard
    Cell Biology Unit, Centre National de la Recherche Scientifique Institut National de la Santé et de la Recherche Médicale, Montpellier, France
    J Cell Biol 118:785-94. 1992
    ..affinity-purified antibodies raised against a synthetic peptide corresponding to the catalytic site of the cdc25 phosphatase, we show that cdc25 protein is constitutively expressed throughout the cell cycle of nontransformed ..
  98. ncbi Structural characterization of the As/Sb reductase LmACR2 from Leishmania major
    Rita Mukhopadhyay
    Department of Biochemistry and Molecular Biology, School of Medicine, Wayne State University, 540 East Canfield Avenue, Detroit, MI 48201, USA
    J Mol Biol 386:1229-39. 2009
    ..are homologous to the catalytic domain of Cdc25 phosphatases, which, in turn, belong to the rhodanese/Cdc25 phosphatase superfamily...
  99. pmc DNA damage and replication checkpoints in fission yeast require nuclear exclusion of the Cdc25 phosphatase via 14-3-3 binding
    Y Zeng
    Department of Cell Biology, Washington University School of Medicine, St Louis, Missouri 63110 1093, USA
    Mol Cell Biol 19:7410-9. 1999
    ..This is accomplished in part by maintaining the Cdc25 phosphatase in a phosphorylated form that binds 14-3-3 proteins...
  100. ncbi Interactions between 2-fluoroadenine 9-beta-D-arabinofuranoside and the kinase inhibitor UCN-01 in human leukemia and lymphoma cells
    S Harvey
    Department of Medicine, Medical College of Virginia, Virginia Commonwealth University, Richmond 23298, USA
    Clin Cancer Res 7:320-30. 2001
    ..Coadministration of UCN-01 with F-ara-A was also associated with diminished phosphorylation of the cdc25 phosphatase. In contrast, exposure of cells to the sequence UCN-01, followed by F-ara-A, resulted in only a modest ..
  101. ncbi MKP-3, a novel cytosolic protein-tyrosine phosphatase that exemplifies a new class of mitogen-activated protein kinase phosphatase
    M Muda
    Glaxo Institute for Molecular Biology, CH 1228 Plan les Ouates, Geneva, Switzerland
    J Biol Chem 271:4319-26. 1996
    ..X is any amino acid) as well as two N-terminal CH2 domains displaying homology to the cell cycle regulator Cdc25 phosphatase. When expressed in COS-7 cells, MKP-3 blocks both the phosphorylation and enzymatic activation of ERK2 by ..

Research Grants26

  1. REGULATION OF MEIOSIS IN SPERMATOGENESIS
    Steven Wasserman; Fiscal Year: 2003
    ..in spermatogenesis has not been defined, Boule has been shown to be essential for translation of the cdc25 phosphatase Twine, a key component of the cell cycle machinery...
  2. THE ROLE OF PIN1 IN MITOSIS WITH DESIGNED INHIBITORS
    FELICIA ETZKORN; Fiscal Year: 2002
    ..Cdc25 specifically is known to interact with Pin1 through two phosphoserine-proline motifs. Cdc25 phosphatase regulates the activity of the mitosis-specific kinase, Cdc2 complexed with cyclin B...
  3. 14 3 3 Implications in Topoisomerase II Pharmacology
    David Kroll; Fiscal Year: 2003
    ..is in directing the subcellular compartmentalization of other proteins: either in nuclear export (for cdc25 phosphatase) or, conversely, in nuclear import (for telomerase and the homeobox transcription factor, TLX-2)...
  4. Cell Cycle G2/M Pathway Modulated by Viral Protein R
    Richard Zhao; Fiscal Year: 2006
    ..is phosphorylated by Wee1 and Mik1 tyrosine kinases during late G2 and is rapidly dephosphorylated by the Cdc25 phosphatase to trigger entry into mitosis...
  5. The Role of Phosphorylation in Regulating Mih1/Cdc25 Activity in Yeast
    VU THUONG THAI; Fiscal Year: 2010
    ..The Cdc25 phosphatase promotes entry into mitosis by removing the inhibitory phosphate place on Cdk1 by the Wee1 kinase...
  6. Molecular mechanisms controlling entry into mitosis
    Douglas Kellogg; Fiscal Year: 2007
    ..In fission yeast, the Wee1 kinase and the Cdc25 phosphatase are required for coordination of cell growth and cell division at G2/M...
  7. PREDOCTORAL FELLOWSHIPS FOR STUDENTS WITH DISABILITIES
    ARON MARQUITZ; Fiscal Year: 2006
    ..Specifically, the budding yeast homologs of the Wee1 kinase and Cdc25 phosphatase are key players in the morphogenesis checkpoint as well...
  8. CONTROL OF CELL DIVISION IN XENOPUS OOCYTES
    JAMES MALLER; Fiscal Year: 2003
    ..This cycling reflects periodic changes in cyclin synthesis and degradation,. and periodic activation of the Cdc25 phosphatase by phosphorylation...
  9. MECHANISM, SPECIFICITY & REGULATION: CDC25 PHOSPHATASES
    Johannes Rudolph; Fiscal Year: 2004
    ..First, the detailed reaction mechanism of the Cdc25 phosphatase with their natural substrates, the Cdk/cyclins will be elucidated...
  10. Chemical Complementation Assay for MKP-1 (RMI)
    JOHN LAZO; Fiscal Year: 2005
    ..This proposal will provide sufficient reagents, including cells and DMA transfection vectors, to permit the high throughput screening of 100,000 compounds. ..
  11. In Vitro High Throughput Screening Assay for MKP-3(RMI)
    JOHN LAZO; Fiscal Year: 2005
    ..Nonetheless, no potent or selective small molecule inhibitors of this protein phosphatase have been identified. This proposal will provide sufficient reagents to permit a high throughput screening of 100,000 compounds ..
  12. Chemical Complementation Assay for MKP-3 (RMI)
    JOHN LAZO; Fiscal Year: 2005
    ..This proposal will provide sufficient reagents, including cells and DMA transfection vectors, to permit the high throughput screening of 100,000 compounds. ..
  13. BIOCHEMICAL REGULATORS OF BLEOMYCIN INDUCED TUMORS
    JOHN LAZO; Fiscal Year: 2001
    ..The Specific Aims are to: 1) develop cells and mice deficient in BH, 2) develop cells and mice that overexpress BH, 3) isolate and characterize potential proteins that interact with BH and, 4) analyze the regulation of BH expression. ..
  14. Pittsburgh Molecular Libraries Screening Center(RMI)
    JOHN LAZO; Fiscal Year: 2008
    ..unreadable] [unreadable]..
  15. In Vitro High Throughput Screening Assay for MKP-1(RMI)
    JOHN LAZO; Fiscal Year: 2005
    ..No potent or selective small molecule inhibitors of this protein phosphatase are known. This proposal will provide sufficient reagents to permit a high throughput screening of 100,000 compounds. ..
  16. Bleomycin Hydrolase and Alzheimer's Disease
    JOHN LAZO; Fiscal Year: 2004
    ..These studies should provide new mechanistic information concerning the pathogenesis of AD as well as reagents for future therapeutic studies. ..
  17. TGFBeta Signaling in the Bladder Stroma
    Neil Bhowmick; Fiscal Year: 2005
    ..Results from these studies are directly relevant to potential non-invasive strategies in treating one of the most common pathologies of the bladder. ..
  18. Biochemical markers of bone turnover in metastatic prostate cancer
    Primo N Lara; Fiscal Year: 2010
    ....
  19. MECHANISMS OF NUCLEAR ENVELOPE BREAKDOWN
    Mark Terasaki; Fiscal Year: 2004
    ..These studies should provide information about the fundamental question of the regulation of the cell cycle. ..
  20. DNA Repair and Checkpoint Interactions in Mammals
    CLARE MCGOWAN; Fiscal Year: 2008
    ..abstract_text> ..
  21. GERM LINE ESTABLISHMENT IN DROSOPHILA MELANOGASTER
    Paul Lasko; Fiscal Year: 2009
    ..Finally, in the 5th aim the relationship between Tud, a protein essential downstream of Vas for germ line establishment, Cup, a translational regulator, TACC, a microtubule binding protein, and Dynein heavy chain will be examined. ..
  22. Bioactive Food Comoponents, Alternative Medicine and Cancer Chemoprevention: Rece
    Shivendra Singh; Fiscal Year: 2007
    ..The theme of our symposium is highly focused and attracts leaders and highly accomplished scientists to discuss promises and challenges in cancer chemoprevention research. [unreadable] [unreadable] [unreadable] [unreadable]..
  23. G2 CHECKPOINT CONTROL IN TRANSFORMED HUMAN CELLS
    CLARE MCGOWAN; Fiscal Year: 2008
    ..abstract_text> ..
  24. Role of Bax and pH in Death by Cytokine Withdrawal
    Annette Khaled; Fiscal Year: 2005
    ....
  25. IL-7 and Lymphocyte Homeostasis: Life versus Death
    Annette Khaled; Fiscal Year: 2009
    ..Expected findings will have significant impact in field of cytokine research. ..