signal recognition particle

Summary

Summary: A cytosolic ribonucleoprotein complex that acts to induce elongation arrest of nascent presecretory and membrane proteins until the ribosome becomes associated with the rough endoplasmic reticulum. It consists of a 7S RNA and at least six polypeptide subunits (relative molecular masses 9, 14, 19, 54, 68, and 72K).

Top Publications

  1. pmc Biogenesis of the signal recognition particle (SRP) involves import of SRP proteins into the nucleolus, assembly with the SRP-RNA, and Xpo1p-mediated export
    H Grosshans
    Biochemie Zentrum Heidelberg, D 69120 Heidelberg, Germany
    J Cell Biol 153:745-62. 2001
  2. pmc Membrane protein insertion at the endoplasmic reticulum
    Sichen Shao
    Cell Biology and Metabolism Program, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA
    Annu Rev Cell Dev Biol 27:25-56. 2011
  3. pmc Signal recognition particle: an essential protein-targeting machine
    David Akopian
    Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, California 91125, USA
    Annu Rev Biochem 82:693-721. 2013
  4. ncbi The signal recognition particle
    R J Keenan
    Maxygen, 515 Galveston Drive, Redwood City, California 94063, USA
    Annu Rev Biochem 70:755-75. 2001
  5. pmc Signal recognition particle components in the nucleolus
    J C Politz
    Department of Biochemistry, University of Massachusetts Medical School, 377 Plantation Street, Suite 337, Worcester, MA 01605, USA
    Proc Natl Acad Sci U S A 97:55-60. 2000
  6. pmc Molecular mechanism of co-translational protein targeting by the signal recognition particle
    Ishu Saraogi
    Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, CA 91125, USA
    Traffic 12:535-42. 2011
  7. pmc Defining the specificity of cotranslationally acting chaperones by systematic analysis of mRNAs associated with ribosome-nascent chain complexes
    Marta del Alamo
    Department of Biology and BioX Program, Stanford University, Stanford, California, United States of America
    PLoS Biol 9:e1001100. 2011
  8. pmc Hierarchical assembly of the Alu domain of the mammalian signal recognition particle
    O Weichenrieder
    European Molecular Biology Laboratory, Grenoble Outstation, France
    RNA 7:731-40. 2001
  9. pmc Signal sequence-independent SRP-SR complex formation at the membrane suggests an alternative targeting pathway within the SRP cycle
    David Braig
    Institut fur Biochemie und Molekularbiologie, ZBMZ, 79104 Freiburg, Germany
    Mol Biol Cell 22:2309-23. 2011
  10. pmc Subunits of the Saccharomyces cerevisiae signal recognition particle required for its functional expression
    J D Brown
    Department of Biochemistry and Biophysics, University of California at San Francisco 94143 0448
    EMBO J 13:4390-400. 1994

Research Grants

  1. MEMBRANES OF THE DENTAL PATHOGEN STREPTOCOCCUS MUTANS
    L Jeannine Brady; Fiscal Year: 2013
  2. Studies of YlxM of Streptococcus mutans, a newly identified component of the Sign
    MATTHEW LAWRENCE WILLIAMS; Fiscal Year: 2013
  3. Membrane Protein Folding and Assembly
    Stephen H White; Fiscal Year: 2013
  4. Roles of Streptococcus mutans YidC1 and YidC2 in membrane biogenesis
    SARA RASER PALMER; Fiscal Year: 2010
  5. Synapt G2-S System with HXMS Automation
    Elizabeth A Komives; Fiscal Year: 2013
  6. mRNA Localization in Organelle Biogenesis
    Christopher V Nicchitta; Fiscal Year: 2013
  7. MECHANISM OF PROTEIN TRANSLOCATION ACROSS MEMBRANES
    Peter Walter; Fiscal Year: 2013
  8. Molecular Basis of Tail-Anchored Membrane Protein Targeting
    Robert J Keenan; Fiscal Year: 2013
  9. Targeting of tail-anchored membrane proteins by the Get pathway.
    William M Clemons; Fiscal Year: 2013
  10. Role of the TOC complex in plastid protein import
    Danny J Schnell; Fiscal Year: 2013

Detail Information

Publications268 found, 100 shown here

  1. pmc Biogenesis of the signal recognition particle (SRP) involves import of SRP proteins into the nucleolus, assembly with the SRP-RNA, and Xpo1p-mediated export
    H Grosshans
    Biochemie Zentrum Heidelberg, D 69120 Heidelberg, Germany
    J Cell Biol 153:745-62. 2001
    The signal recognition particle (SRP) targets nascent secretory proteins to the ER, but how and where the SRP assembles is largely unknown...
  2. pmc Membrane protein insertion at the endoplasmic reticulum
    Sichen Shao
    Cell Biology and Metabolism Program, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA
    Annu Rev Cell Dev Biol 27:25-56. 2011
    ..The classical cotranslational pathway, utilized by most membrane proteins, involves targeting by the signal recognition particle followed by insertion via the Sec61 translocon...
  3. pmc Signal recognition particle: an essential protein-targeting machine
    David Akopian
    Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, California 91125, USA
    Annu Rev Biochem 82:693-721. 2013
    The signal recognition particle (SRP) and its receptor compose a universally conserved and essential cellular machinery that couples the synthesis of nascent proteins to their proper membrane localization...
  4. ncbi The signal recognition particle
    R J Keenan
    Maxygen, 515 Galveston Drive, Redwood City, California 94063, USA
    Annu Rev Biochem 70:755-75. 2001
    The signal recognition particle (SRP) and its membrane-associated receptor (SR) catalyze targeting of nascent secretory and membrane proteins to the protein translocation apparatus of the cell...
  5. pmc Signal recognition particle components in the nucleolus
    J C Politz
    Department of Biochemistry, University of Massachusetts Medical School, 377 Plantation Street, Suite 337, Worcester, MA 01605, USA
    Proc Natl Acad Sci U S A 97:55-60. 2000
    The signal recognition particle (SRP) is a ribonucleoprotein composed of an Alu domain and an S domain. The S domain contains unique sequence SRP RNA and four SRP proteins: SRP19, SRP54, SRP68, and SRP72...
  6. pmc Molecular mechanism of co-translational protein targeting by the signal recognition particle
    Ishu Saraogi
    Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, CA 91125, USA
    Traffic 12:535-42. 2011
    The signal recognition particle (SRP) is a key component of the cellular machinery that couples the ongoing synthesis of proteins to their proper localization, and has often served as a paradigm for understanding the molecular basis of ..
  7. pmc Defining the specificity of cotranslationally acting chaperones by systematic analysis of mRNAs associated with ribosome-nascent chain complexes
    Marta del Alamo
    Department of Biology and BioX Program, Stanford University, Stanford, California, United States of America
    PLoS Biol 9:e1001100. 2011
    ..We here focused on two Saccharomyces cerevisiae chaperones: the Signal Recognition Particle (SRP), which acts cotranslationally to target proteins to the ER, and the Nascent chain Associated ..
  8. pmc Hierarchical assembly of the Alu domain of the mammalian signal recognition particle
    O Weichenrieder
    European Molecular Biology Laboratory, Grenoble Outstation, France
    RNA 7:731-40. 2001
    The mammalian signal recognition particle (SRP) catalytically promotes cotranslational translocation of signal sequence containing proteins across the endoplasmic reticulum membrane...
  9. pmc Signal sequence-independent SRP-SR complex formation at the membrane suggests an alternative targeting pathway within the SRP cycle
    David Braig
    Institut fur Biochemie und Molekularbiologie, ZBMZ, 79104 Freiburg, Germany
    Mol Biol Cell 22:2309-23. 2011
    Protein targeting by the signal recognition particle (SRP) and the bacterial SRP receptor FtsY requires a series of closely coordinated steps that monitor the presence of a substrate, the membrane, and a vacant translocon...
  10. pmc Subunits of the Saccharomyces cerevisiae signal recognition particle required for its functional expression
    J D Brown
    Department of Biochemistry and Biophysics, University of California at San Francisco 94143 0448
    EMBO J 13:4390-400. 1994
    The signal recognition particle (SRP) is an evolutionarily conserved ribonucleoprotein (RNP) complex that functions in protein targeting to the endoplasmic reticulum (ER) membrane...
  11. ncbi Protein targeting by the signal recognition particle
    Przemyslaw Grudnik
    Heidelberg University Biochemistry Center BZH, Im Neuenheimer Feld 328, D 69120 Heidelberg, Germany
    Biol Chem 390:775-82. 2009
    Protein targeting by the signal recognition particle (SRP) is universally conserved and starts with the recognition of a signal sequence in the context of a translating ribosome...
  12. ncbi An E. coli ribonucleoprotein containing 4.5S RNA resembles mammalian signal recognition particle
    M A Poritz
    Department of Biochemistry and Biophysics, University of California Medical School, San Francisco 94143 0448
    Science 250:1111-7. 1990
    The signal recognition particle (SRP) plays a central role in directing the export of nascent proteins from the cytoplasm of mammalian cells...
  13. pmc Signal sequence- and translation-independent mRNA localization to the endoplasmic reticulum
    Brook Pyhtila
    Department of Cell Biology, Duke University Medical Center, Durham, North Carolina 27710, USA
    RNA 14:445-53. 2008
    ..to the endoplasmic reticulum (ER), in contrast, occurs via a co-translational, signal sequence/signal recognition particle (SRP)-dependent mechanism...
  14. pmc Signal recognition particle (SRP) and SRP receptor: a new paradigm for multistate regulatory GTPases
    Shu ou Shan
    Division of Chemistry and Chemical Engineering, California Institute of Technology, 1200 East California Boulevard, Pasadena, California 91125, USA
    Biochemistry 48:6696-704. 2009
    ..We suggest that these multistate regulatory GTPases are uniquely suited to provide spatial and temporal control of complex cellular pathways that require multiple molecular events to occur in a highly coordinated fashion...
  15. pmc The crystal structure of the signal recognition particle in complex with its receptor
    Sandro F Ataide
    Institute of Molecular Biology and Biophysics, Eidgenössische Technische Hochschule Zurich ETH Zurich, Zurich, Switzerland
    Science 331:881-6. 2011
    Cotranslational targeting of membrane and secretory proteins is mediated by the universally conserved signal recognition particle (SRP)...
  16. ncbi Inefficient targeting to the endoplasmic reticulum by the signal recognition particle elicits selective defects in post-ER membrane trafficking
    Asvin K K Lakkaraju
    Department of Cell Biology, University of Geneva, Sciences III, CH 1211 Geneva 4, Switzerland
    Exp Cell Res 313:834-47. 2007
    The signal recognition particle (SRP) is required for protein translocation into the endoplasmic reticulum (ER). With RNA interference we reduced its level about ten-fold in mammalian cells to study its cellular functions...
  17. pmc Alu RNP and Alu RNA regulate translation initiation in vitro
    Julien Häsler
    Departement de Biologie Cellulaire, Universite de Geneve, 30 Quai Ernest Ansermet, 1211 Geneve 4, Switzerland
    Nucleic Acids Res 34:2374-85. 2006
    Alu elements are the most abundant repetitive elements in the human genome; they emerged from the signal recognition particle RNA gene and are composed of two related but distinct monomers (left and right arms)...
  18. ncbi Identification of a targeting factor for posttranslational membrane protein insertion into the ER
    Sandra Stefanovic
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Cell 128:1147-59. 2007
    ..Thus, TRC40/Asna-1 represents an integral component of a posttranslational pathway of membrane protein insertion whose targeting is mediated by TRC...
  19. pmc Sequential checkpoints govern substrate selection during cotranslational protein targeting
    Xin Zhang
    Division of Chemistry and Chemical Engineering, California Institute of Technology, 1200 East California Boulevard, Pasadena, CA 91125, USA
    Science 328:757-60. 2010
    ..To address this fundamental question, we investigated the signal recognition particle (SRP) pathway in Escherichia coli, which delivers proteins to the bacterial inner membrane through ..
  20. ncbi Classification and evolution of P-loop GTPases and related ATPases
    Detlef D Leipe
    National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
    J Mol Biol 317:41-72. 2002
    ..The second class, designated SIMIBI (after signal recognition particle, MinD, and BioD), consists of signal recognition particle (SRP) GTPases, the assemblage of MinD-like ..
  21. pmc The tmRDB and SRPDB resources
    Ebbe Sloth Andersen
    Department of Molecular Biology, University of Aarhus, C F Moellers Alle, Building 139, 8000 Aarhus, Denmark
    Nucleic Acids Res 34:D163-8. 2006
    ..kvl.dk/). The signal recognition particle database (SRPDB) at http://psyche.uthct.edu/dbs/SRPDB/SRPDB.html is mirrored at http://srpdb.kvl...
  22. ncbi Sequence-specific interactions of nascent Escherichia coli polypeptides with trigger factor and signal recognition particle
    Ronald S Ullers
    Department of Molecular Microbiology, Institute of Molecular Cell Biology, Vrije Universiteit, 1081 HV Amsterdam, The Netherlands
    J Biol Chem 281:13999-4005. 2006
    ..The signal recognition particle (SRP) effectively competes the contact with TF when a signal anchor (SA) sequence of a nascent inner ..
  23. ncbi Role of SRP RNA in the GTPase cycles of Ffh and FtsY
    P Peluso
    Howard Hughes Medical Institute and Department of Biochemistry and Biophysics, University of California at San Francisco, 94143 0448, USA
    Biochemistry 40:15224-33. 2001
    The bacterial homologues of the signal recognition particle (SRP) and its receptor, the Ffh*4...
  24. ncbi Nascent membrane and presecretory proteins synthesized in Escherichia coli associate with signal recognition particle and trigger factor
    Q A Valent
    Department of Microbiology, Institute of Molecular Biological Sciences, BioCentrum Amsterdam, The Netherlands
    Mol Microbiol 25:53-64. 1997
    The Escherichia coli signal recognition particle (SRP) and trigger factor are cytoplasmic factors that interact with short nascent polypeptides of presecretory and membrane proteins produced in a heterologous in vitro translation system...
  25. pmc 7SL RNA, but not the 54-kd signal recognition particle protein, is an abundant component of both infectious HIV-1 and minimal virus-like particles
    Adewunmi A Onafuwa-Nuga
    RNA 12:542-6. 2006
    The virion incorporation of 7SL, the RNA component of the host signal recognition particle (SRP), has been shown for several simple retroviruses...
  26. ncbi Origins and evolution of cotranslational transport to the ER
    Thomas U Schwartz
    Department of Biology, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA
    Adv Exp Med Biol 607:52-60. 2007
    ..first, an N-terminal signal sequence of a nascent polypeptide is recognized on the ribosome by the signal recognition particle (SRP), then the SRP-ribosome complex is targeted to the membrane via the SRP receptor...
  27. pmc Assembly of the Alu domain of the signal recognition particle (SRP): dimerization of the two protein components is required for efficient binding to SRP RNA
    K Strub
    Department of Biochemistry and Biophysics, University of California Medical School, San Francisco 94143 0448
    Mol Cell Biol 10:777-84. 1990
    The signal recognition particle (SRP), a cytoplasmic ribonucleoprotein, plays an essential role in targeting secretory proteins to the rough endoplasmic reticulum membrane...
  28. pmc Transient tether between the SRP RNA and SRP receptor ensures efficient cargo delivery during cotranslational protein targeting
    Kuang Shen
    Division of Chemistry and Chemical Engineering, California Institute of Technology, 1200 E California Boulevard, Pasadena, CA 91125, USA
    Proc Natl Acad Sci U S A 107:7698-703. 2010
    ..An example of such control occurs in cotranslational protein targeting by the signal recognition particle (SRP), during which the SRP RNA and the cargo both accelerate complex assembly between the SRP and SRP ..
  29. pmc Demonstration of a multistep mechanism for assembly of the SRP x SRP receptor complex: implications for the catalytic role of SRP RNA
    Xin Zhang
    Division of Chemistry and Chemical Engineering, California Institute of Technology, 1200 East California Boulevard, Pasadena, CA 91125, USA
    J Mol Biol 381:581-93. 2008
    Two GTPases in the signal recognition particle (SRP) and its receptor (SR) control the delivery of newly synthesized proteins to the endoplasmic reticulum or plasma membrane. During the protein targeting reaction, the 4...
  30. pmc Interplay of signal recognition particle and trigger factor at L23 near the nascent chain exit site on the Escherichia coli ribosome
    Ronald S Ullers
    Department of Molecular Microbiology, Vrije Universiteit, 1081 HV Amsterdam, The Netherlands
    J Cell Biol 161:679-84. 2003
    ..The targeting factor signal recognition particle (SRP) interacts specifically with the signal anchor (SA) sequence in nascent inner membrane proteins (..
  31. pmc The SRP9/14 subunit of the signal recognition particle (SRP) is present in more than 20-fold excess over SRP in primate cells and exists primarily free but also in complex with small cytoplasmic Alu RNAs
    F Bovia
    Universite de Geneve, Departement de Biologie Cellulaire, Switzerland
    Mol Biol Cell 6:471-84. 1995
    ..bound to the Alu sequences of SRP RNA is essential for the translational control function of the signal recognition particle (SRP)...
  32. pmc Functional substitution of the signal recognition particle 54-kDa subunit by its Escherichia coli homolog
    H D Bernstein
    Department of Biochemistry and Biophysics, University of California Medical School, San Francisco 94143 0448
    Proc Natl Acad Sci U S A 90:5229-33. 1993
    The 54-kDa subunit of the mammalian signal recognition particle (SRP54) binds to the signal sequences of nascent secretory and transmembrane proteins and facilitates their cotranslational targeting to the membrane translocation apparatus ..
  33. ncbi Roles for Srp72p in assembly, nuclear export and function of the signal recognition particle
    Rob W van Nues
    Institute for Cell and Molecular Biosciences, The Medical School, Newcastle University, Newcastle upon Tyne, UK
    RNA Biol 5:73-83. 2008
    Co-translational protein targeting to the endoplasmic reticulum is catalysed by the signal recognition particle, a conserved ribonucleoprotein...
  34. ncbi Message on the web: mRNA and ER co-trafficking
    Jeffrey E Gerst
    Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel
    Trends Cell Biol 18:68-76. 2008
    ..e. during cell division in yeast), or enable confined spatial responses to environmental stimuli (i.e. during synaptic remodeling or in cases of neuronal injury)...
  35. ncbi Signal recognition particle assembly in relation to the function of amplified nucleoli of Xenopus oocytes
    John Sommerville
    Division of Cell and Molecular Biology, School of Biology, University of St Andrews, KY16 9TS, UK
    J Cell Sci 118:1299-307. 2005
    The signal recognition particle (SRP) is a ribonucleoprotein machine that controls the translation and intracellular sorting of membrane and secreted proteins. The SRP contains a core RNA subunit with which six proteins are assembled...
  36. pmc Interaction studies between the chloroplast signal recognition particle subunit cpSRP43 and the full-length translocase Alb3 reveal a membrane-embedded binding region in Alb3 protein
    Beatrix Dünschede
    Molecular Biology of Plant Organelles, Ruhr University Bochum, 44780 Bochum, Germany
    J Biol Chem 286:35187-95. 2011
    ..of the light-harvesting chlorophyll a,b-binding proteins depends on the function of the chloroplast signal recognition particle, its receptor cpFtsY, and the translocase Alb3...
  37. ncbi Is there a twist in the Escherichia coli signal recognition particle pathway?
    Eitan Bibi
    Department of Biological Chemistry, The Weizmann Institute of Science, Rehovot 76100, Israel
    Trends Biochem Sci 37:1-6. 2012
    ..is that ribosomes and transcripts are delivered to the membrane together during IMP translation by the signal recognition particle (SRP) and its receptor...
  38. pmc Synergistic actions between the SRP RNA and translating ribosome allow efficient delivery of the correct cargos during cotranslational protein targeting
    Kuang Shen
    Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, California 91125, USA
    RNA 17:892-902. 2011
    During cotranslational protein targeting by the Signal Recognition Particle (SRP), the correct cargo accelerates stable complex assembly between the SRP and SRP receptor (FtsY) by several orders of magnitude, thus enabling rapid and ..
  39. pmc Dicer-dependent biogenesis of small RNAs derived from 7SL RNA
    Yong feng Ren
    Institute of Genomic Medicine, Wenzhou Medical College, Wenzhou, People s Republic of China
    PLoS ONE 7:e40705. 2012
    ..In conclusion, the current study demonstrated that Dicer can process 7SL RNA, however, the biological significance remains to be elucidated...
  40. ncbi A derivative of lipid A is involved in signal recognition particle/SecYEG-dependent and -independent membrane integrations
    Ken ichi Nishiyama
    Institute of Biochemistry and Molecular Biology, Centre of Biochemistry and Molecular Cell Research, University of Freiburg, Hermann Herder Strasse 7, D 79104 Freiburg, Germany
    J Biol Chem 281:35667-76. 2006
    ..of mannitol permease was dependent on both the SecYEG translocon and this factor and was mediated by signal recognition particle and signal recognition particle receptor...
  41. pmc Association of potent human antiviral cytidine deaminases with 7SL RNA and viral RNP in HIV-1 virions
    Wenyan Zhang
    First Affiliated Hospital, Institute of Virology and AIDS Research, Jilin University, Changchun, Jilin Province, People s Republic of China 130062
    J Virol 84:12903-13. 2010
    7SL RNA promotes the formation of the signal recognition particle that targets secretory and membrane proteins to the endoplasmic reticulum. 7SL RNA is also selectively packaged by many retroviruses, including HIV-1...
  42. pmc Genetic evidence for functional interaction of the Escherichia coli signal recognition particle receptor with acidic lipids in vivo
    Elinor Erez
    Department of Biological Chemistry, The Weizmann Institute of Science, Rehovot 76100, Israel
    J Biol Chem 285:40508-14. 2010
    The mechanism underlying the interaction of the Escherichia coli signal recognition particle receptor FtsY with the cytoplasmic membrane has been studied in detail...
  43. pmc Myopathy with antibodies to the signal recognition particle: clinical and pathological features
    T Miller
    Washington University School of Medicine, Department of Neurology, St Louis, Missouri 63110, USA
    J Neurol Neurosurg Psychiatry 73:420-8. 2002
    To study myopathies with serum antibodies to the signal recognition particle (SRP), an unusual, myositis specific antibody associated syndrome that has not been well characterised pathologically.
  44. pmc Signal recognition particle mediates post-translational targeting in eukaryotes
    Benjamin M Abell
    School of Biological Sciences, University of Manchester, Manchester, UK
    EMBO J 23:2755-64. 2004
    b>Signal recognition particle (SRP) plays a central role in the delivery of classical secretory and membrane proteins to the endoplasmic reticulum (ER)...
  45. pmc A signal-anchor sequence stimulates signal recognition particle binding to ribosomes from inside the exit tunnel
    Uta Berndt
    Institute of Biochemistry and Molecular Biology and Center for Biological Signalling Studies, University of Freiburg, Stefan Meier Strasse 17, D 79104 Freiburg, Germany
    Proc Natl Acad Sci U S A 106:1398-403. 2009
    ..and secretory proteins depends on recognition of ribosome-bound nascent chain signal sequences by the signal recognition particle (SRP)...
  46. ncbi Signal sequence recognition and protein targeting
    R M Stroud
    S 960 Department of Biochemistry and Biophysics, School of Medicine, University of California, San Francisco CA 94143 0448, USA
    Curr Opin Struct Biol 9:754-9. 1999
    ..determined structures suggest how signal sequences are recognized and how the GTP affinities of the signal recognition particle and its receptor are coupled to the targeting of ribosomes to translocational membrane pores...
  47. ncbi An in vitro assay using overexpressed yeast SRP demonstrates that cotranslational translocation is dependent upon the J-domain of Sec63p
    Martin Willer
    School of Biological Sciences, 2 205 Stopford Building, University of Manchester, Oxford Road, Manchester M13 9PT, UK
    Biochemistry 42:7171-7. 2003
    The signal recognition particle (SRP) is required for co-translational targeting of polypeptides to the endoplasmic reticulum (ER)...
  48. pmc Access to ribosomal protein Rpl25p by the signal recognition particle is required for efficient cotranslational translocation
    Jane A Dalley
    Faculty of Life Sciences, University of Manchester, Manchester, M13 9PT, United Kingdom
    Mol Biol Cell 19:2876-84. 2008
    ..to the endoplasmic reticulum (ER) occurs cotranslationally necessitating the interaction of the signal recognition particle (SRP) and the translocon with the ribosome...
  49. pmc Membrane composition changes and physiological adaptation by Streptococcus mutans signal recognition particle pathway mutants
    Adnan Hasona
    Department of Oral Biology, University of Florida, Gainesville, FL 32610, USA
    J Bacteriol 189:1219-30. 2007
    ..impaired and sensitive to environmental stress when genes encoding the minimal conserved bacterial signal recognition particle (SRP) elements are inactivated...
  50. ncbi Pathways for compartmentalizing protein synthesis in eukaryotic cells: the template-partitioning model
    Christopher V Nicchitta
    Department of Cell Biology, Duke University Medical Center, Durham, NC 27710, USA
    Biochem Cell Biol 83:687-95. 2005
    ..In this review, key findings on this topic are discussed, and the template-partitioning model, describing a hypothetical mechanism for RNA partitioning in the eukaryotic cell, is proposed...
  51. ncbi The E. coli ffh gene is necessary for viability and efficient protein export
    G J Phillips
    Department of Biology, College of William and Mary, Williamsburg, Virginia 23185
    Nature 359:744-6. 1992
    Homologues of the gene encoding the 54K (M(r) 54,000) subunit of the mammalian signal recognition particle have been identified in different organisms...
  52. pmc The 4.5S RNA component of the signal recognition particle is required for group A Streptococcus virulence
    Jeanette Treviño
    Center for Molecular and Translational Human Infectious Diseases Research, The Methodist Hospital Research Institute, Houston, TX 77030, USA
    Microbiology 156:1342-50. 2010
    The signal recognition particle (SRP) is a ribonucleoprotein complex that targets proteins for secretion in a co-translational manner...
  53. pmc 7SL RNA is retained in HIV-1 minimal virus-like particles as an S-domain fragment
    Sarra E Keene
    Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, Michigan, USA
    J Virol 84:9070-7. 2010
    ....
  54. ncbi The bacterial SRP receptor, SecA and the ribosome use overlapping binding sites on the SecY translocon
    Patrick Kuhn
    Institut fur Biochemie und Molekularbiologie, ZBMZ, Stefan Meier Str 17, D 79104 Freiburg, Germany
    Traffic 12:563-78. 2011
    b>Signal recognition particle (SRP)-dependent protein targeting is a universally conserved process that delivers proteins to the bacterial cytoplasmic membrane or to the endoplasmic reticulum membrane in eukaryotes...
  55. pmc Co-translational protein targeting catalyzed by the Escherichia coli signal recognition particle and its receptor
    T Powers
    Department of Biochemistry and Biophysics, University of California, School of Medicine, San Francisco 94143, USA
    EMBO J 16:4880-6. 1997
    ..particle (RNP) and FtsY from Escherichia coli are homologous to essential components of the mammalian signal recognition particle (SRP) and SRP receptor, respectively. The ability of these E...
  56. ncbi Delivering proteins for export from the cytosol
    Benedict C S Cross
    Faculty of Life Sciences, University of Manchester, Michael Smith Building, Oxford Road, Manchester, M13 9PT, UK
    Nat Rev Mol Cell Biol 10:255-64. 2009
    ..These distinct pathways provide different solutions for the maintenance of proteins in a state that is competent for membrane translocation and their delivery for export from the cytosol...
  57. ncbi Double-spanning plant viral movement protein integration into the endoplasmic reticulum membrane is signal recognition particle-dependent, translocon-mediated, and concerted
    Ana Sauri
    Departament de Bioquimica i Biologia Molecular, Universitat de València E 46 100 Burjassot, Spain
    J Biol Chem 280:25907-12. 2005
    ..We have discovered that this process is cotranslational and proceeds in a signal recognition particle-dependent manner...
  58. ncbi Nascent-polypeptide-associated complex
    S Rospert
    Max Planck Research Unit for Enzymology of Protein Folding, Weinbergweg 22, 06120 Halle, Germany
    Cell Mol Life Sci 59:1632-9. 2002
    ..This review attempts to summarize the data from different fields and to discuss the partly controversial results in a common context...
  59. ncbi Structural basis of signal-sequence recognition by the signal recognition particle
    Tobias Hainzl
    Department of Chemistry, Umea University, Umea, Sweden
    Nat Struct Mol Biol 18:389-91. 2011
    The signal recognition particle (SRP) recognizes and binds the signal sequence of nascent proteins as they emerge from the ribosome. We present here the 3.0-Å structure of a signal sequence bound to the Methanococcus jannaschii SRP core...
  60. pmc Streptococcal viability and diminished stress tolerance in mutants lacking the signal recognition particle pathway or YidC2
    Adnan Hasona
    Department of Oral Biology, University of Florida, Gainesville, FL 32610, USA
    Proc Natl Acad Sci U S A 102:17466-71. 2005
    The signal recognition particle (SRP)-translocation pathway is conserved in all three domains of life and delivers membrane and secretory proteins to the cytoplasmic membrane or endoplasmic reticulum...
  61. pmc Component specificity for the thylakoidal Sec and Delta pH-dependent protein transport pathways
    H Mori
    Horticultural Sciences and Plant Molecular and Cellular Biology Program, University of Florida, Gainesville, Florida 32611, USA
    J Cell Biol 146:45-56. 1999
    ..These include the Sec, signal recognition particle (SRP), and Delta pH/Tat systems...
  62. ncbi Structure of the SRP19 RNA complex and implications for signal recognition particle assembly
    Tobias Hainzl
    Umea Centre for Molecular Pathogenesis, Umea University, SE 901 87 Umea, Sweden
    Nature 417:767-71. 2002
    The signal recognition particle (SRP) is a phylogenetically conserved ribonucleoprotein. It associates with ribosomes to mediate co-translational targeting of membrane and secretory proteins to biological membranes...
  63. ncbi Anti-signal recognition particle autoantibody in patients with and patients without idiopathic inflammatory myopathy
    Amy H Kao
    University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15261, USA
    Arthritis Rheum 50:209-15. 2004
    To determine the long-term outcome and associated clinical, serologic, and pathologic features in a cohort of patients with connective tissue disease (CTD) and the anti-signal recognition particle (anti-SRP) autoantibody.
  64. pmc Anti-signal recognition particle autoantibodies: marker of a necrotising myopathy
    G J D Hengstman
    Department of Neurology, Neuromuscular Centre Nijmegen, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands
    Ann Rheum Dis 65:1635-8. 2006
    To elucidate the clinical importance of the anti-signal recognition particle (SRP) autoantibody in patients with myositis.
  65. pmc Differential regulation of the TRAIL death receptors DR4 and DR5 by the signal recognition particle
    Yan Guo Ren
    Department of Cancer Biology, Genomics Institute of the Novartis Research Foundation, San Diego, CA 92121, USA
    Mol Biol Cell 15:5064-74. 2004
    ..DR5-mediated apoptosis. These experiments revealed that expression of the signal recognition particle (SRP) complex is essential for apoptosis mediated by DR4, but not DR5...
  66. pmc Export of the pseudopilin XcpT of the Pseudomonas aeruginosa type II secretion system via the signal recognition particle-Sec pathway
    Jorik Arts
    Department of Molecular Microbiology and Institute of Biomembranes, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands
    J Bacteriol 189:2069-76. 2007
    ..Experiments with conditional sec mutants and reporter-protein fusions showed that this transport process involves the cotranslational signal recognition particle targeting route and is dependent on a functional Sec translocon.
  67. ncbi bicaudal encodes the Drosophila beta NAC homolog, a component of the ribosomal translational machinery*
    D C Markesich
    Department of Biochemistry, Rice University, MS 140, Houston, TX 77005, USA
    Development 127:559-72. 2000
    ..These findings suggest that repression of nanos mRNA translation occurs on the ribosome and involves a role for beta NAC...
  68. ncbi The alpha and beta subunit of the nascent polypeptide-associated complex have distinct functions
    B Beatrix
    Cellular Biochemistry and Biophysics Program, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    J Biol Chem 275:37838-45. 2000
    ..Eventually other factors involved in targeting and folding, like the Signal Recognition Particle or cytosolic chaperones, must gain access to the nascent chain...
  69. ncbi Domain interactions in E. coli SRP: stabilization of M domain by RNA is required for effective signal sequence modulation of NG domain
    N Zheng
    Molecular Biophysics Program, University of Texas Southwestern Medical Center at Dallas 75235, USA
    Mol Cell 1:79-87. 1997
    The E. coli protein, Fth, binds to 4.5S RNA through its M domain to form the signal recognition particle (SRP). The other domain of Fth (NG) is a GTPase, which binds and is coordinately regulated by its receptor, FtsY...
  70. pmc Signal recognition particle-dependent inner membrane targeting of the PulG Pseudopilin component of a type II secretion system
    Olivera Francetic
    Molecular Genetics Unit, Institut Pasteur, 25 rue du Dr Roux, 75724 Paris Cedex 15, France
    J Bacteriol 189:1783-93. 2007
    ..Furthermore, depletion of the Ffh component of the signal recognition particle (SRP) completely abolished PrePulG* processing, without affecting the Sec-dependent export of ..
  71. ncbi Crystal structure of an early protein-RNA assembly complex of the signal recognition particle
    K Wild
    Biochemie Zentrum BZH, University of Heidelberg, Im Neuenheimer Feld 328, D 69120 Heidelberg, Germany
    Science 294:598-601. 2001
    The signal recognition particle (SRP) is a universally conserved ribonucleoprotein complex that mediates the cotranslational targeting of secretory and membrane proteins to cellular membranes...
  72. pmc Residues in SRP9/14 essential for elongation arrest activity of the signal recognition particle define a positively charged functional domain on one side of the protein
    Camille Mary
    Departement de Biologie Cellulaire, Universite de Geneve, Sciences III, 1211 Geneve 4, Switzerland
    RNA 16:969-79. 2010
    The signal recognition particle (SRP) is a ubiquitous cytoplasmic ribonucleoprotein complex required for the cotranslational targeting of proteins to the endoplasmic reticulum (ER)...
  73. ncbi Protein targeting: getting into the groove
    H D Bernstein
    Genetics and Biochemistry Branch, National Institute of Diabetes and Digestive and Kidney Disease, National Institutes of Health Building 10, Room 9D 20, 10 Center Drive, Bethesda, Maryland 20892 1810, USA
    Curr Biol 8:R715-8. 1998
    The 54 kDa subunit of the signal recognition particle has to identify a diverse family of substrates and deliver them in a controlled manner to the translocation machinery of the endoplasmic reticulum...
  74. ncbi Characterization of the consequences of YidC depletion on the inner membrane proteome of E. coli using 2D blue native/SDS-PAGE
    David Wickström
    Center for Biomembrane Research, Department of Biochemistry and Biophysics, Stockholm University, SE 106 91 Stockholm, Sweden
    J Mol Biol 409:124-35. 2011
    ..In conclusion, our analysis identifies many new IMPs/IMP complexes that depend on YidC for their biogenesis, responses that accompany depletion of YidC and an IMP characteristic that is associated with YidC dependence...
  75. pmc The signal recognition particle (SRP) RNA links conformational changes in the SRP to protein targeting
    Niels Bradshaw
    Howard Hughes Medical Institute, Department of Biochemistry and Biophysics, University of California at San Francisco, CA 94158, USA
    Mol Biol Cell 18:2728-34. 2007
    The RNA component of the signal recognition particle (SRP) is universally required for cotranslational protein targeting...
  76. pmc Interactions that drive Sec-dependent bacterial protein transport
    Sharyn L Rusch
    Department of Molecular and Cell Biology, The University of Connecticut, Storrs, Connecticut 06269 3125, USA
    Biochemistry 46:9665-73. 2007
    ....
  77. pmc The Alu domain homolog of the yeast signal recognition particle consists of an Srp14p homodimer and a yeast-specific RNA structure
    K Strub
    Departement de Biologie Cellulaire, Universite de Geneve, Sciences III, Switzerland
    RNA 5:1333-47. 1999
    The mammalian Alu domain of the signal recognition particle (SRP) consists of a heterodimeric protein SRP9/14 and the Alu portion of 7SL RNA and comprises the elongation arrest function of the particle...
  78. pmc Characterization of conserved bases in 4.5S RNA of Escherichia coli by construction of new F' factors
    James M Peterson
    Department of Veterinary Microbiology, Iowa State University, 1802 University Boulevard, Building 6, Ames, IA 50011, USA
    J Bacteriol 190:7709-18. 2008
    ..that correspond to the region of the RNA molecule that interacts directly with Ffh in assembly of the signal recognition particle. To facilitate characterization of the ffs mutations with minimal manipulation, recombineering was used ..
  79. pmc Structure of 4.5S RNA in the signal recognition particle of Escherichia coli as studied by enzymatic and chemical probing
    G Lentzen
    Institut fur Molekularbiologie, Universitat Witten Herdecke, Germany
    RNA 2:244-53. 1996
    The structure of 4.5S RNA, the Escherichia coli homologue of the signal recognition particle (SRP) RNA, alone and in the SRP complex with protein P48 (Ffh) was probed both enzymatically and chemically...
  80. ncbi Streptococcus mutans ffh, a gene encoding a homologue of the 54 kDa subunit of the signal recognition particle, is involved in resistance to acid stress
    J A Gutierrez
    Department of Oral Biology, University of Florida, Gainesville 32610, USA
    Microbiology 145:357-66. 1999
    ..These findings suggest that Ffh may be involved in the maintenance of a functional membrane protein composition during adaptation of S. mutans to changing environmental conditions...
  81. pmc Direct visualization reveals dynamics of a transient intermediate during protein assembly
    Xin Zhang
    Division of Chemistry and Chemical Engineering, California Institute of Technology, 1200 East California Boulevard, Pasadena, CA 91125, USA
    Proc Natl Acad Sci U S A 108:6450-5. 2011
    ..dynamics, and stability of a transient, on-pathway intermediate during complex assembly between the Signal Recognition Particle (SRP) and its receptor...
  82. pmc The Srp54 GTPase is essential for protein export in the fission yeast Schizosaccharomyces pombe
    S M Althoff
    Department of Biochemistry, University of Illinois, Urbana 61801
    Mol Cell Biol 14:7839-54. 1994
    b>Signal recognition particle (SRP) is a cytoplasmic ribonucleoprotein required for targeting a subset of presecretory proteins to the endoplasmic reticulum (ER) membrane...
  83. ncbi Correlation of anti-signal recognition particle autoantibody levels with creatine kinase activity in patients with necrotizing myopathy
    Olivier Benveniste
    Universite Pierre et Marie Curie, Hopital Pitie Salpetriere, Assistance Publique Hopitaux de Paris, Paris, France
    Arthritis Rheum 63:1961-71. 2011
    Anti-signal recognition particle (anti-SRP) autoantibodies are associated with severe acquired necrotizing myopathies. The role of these autoantibodies remains elusive, and the evolution of anti-SRP levels over time is unknown...
  84. pmc Genetic and biochemical analysis of the fission yeast ribonucleoprotein particle containing a homolog of Srp54p
    D Selinger
    Department of Biochemistry, University of Illinois at Urbana Champaign 61801
    Nucleic Acids Res 22:2557-67. 1994
    Mammalian signal recognition particle (SRP), a complex of six polypeptides and one 7SL RNA molecule, is required for targeting nascent presecretory proteins to the endoplasmic reticulum (ER)...
  85. ncbi Structure and function of the chloroplast signal recognition particle
    Danja Schünemann
    Lehrstuhl für Allgemeine und Molekulare Botanik, Ruhr Universitat Bochum, 44780 Bochum, Germany
    Curr Genet 44:295-304. 2004
    ..The signal recognition particle (SRP) is found in the cytoplasm of most, if not all, eukaryotes and prokaryotes where it plays a ..
  86. pmc Translation elongation regulates substrate selection by the signal recognition particle
    Dawei Zhang
    Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, California 91125, USA
    J Biol Chem 287:7652-60. 2012
    The signal recognition particle (SRP) is a universally conserved cellular machinery responsible for delivering membrane and secretory proteins to the proper cellular destination...
  87. ncbi Insertion of PsaK into the thylakoid membrane in a "Horseshoe" conformation occurs in the absence of signal recognition particle, nucleoside triphosphates, or functional albino3
    A Mant
    Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, United Kingdom
    J Biol Chem 276:36200-6. 2001
    ..Insertion of PsaK does not require stromal extract, indicating that signal recognition particle (SRP) is not involved...
  88. ncbi Enhancing the secretory yields of leech carboxypeptidase inhibitor in Escherichia coli: influence of trigger factor and signal recognition particle
    Juan Miguel Puertas
    Department of Chemical Engineering, University of Washington, Seattle, WA 98195 1750, USA
    Protein Expr Purif 74:122-8. 2010
    The signal recognition particle (SRP) dependent secretion pathway is as an attractive alternative to Sec-dependent export for the production of disulfide-bonded and/or fast-folding recombinant proteins in the Escherichia coli periplasm...
  89. pmc The beta subunit of the signal recognition particle receptor is a transmembrane GTPase that anchors the alpha subunit, a peripheral membrane GTPase, to the endoplasmic reticulum membrane
    J D Miller
    Department of Biochemistry and Biophysics, University of California Medical School, San Francisco 94143 0448
    J Cell Biol 128:273-82. 1995
    The signal recognition particle receptor (SR) is required for the cotranslational targeting of both secretory and membrane proteins to the endoplasmic reticulum (ER) membrane...
  90. pmc Consequences of depletion of the signal recognition particle in Escherichia coli
    David Wickström
    Department of Biochemistry and Biophysics, Center for Biomembrane Research, Stockholm University, SE 106 91 Stockholm, Sweden
    J Biol Chem 286:4598-609. 2011
    Thus far, the role of the Escherichia coli signal recognition particle (SRP) has only been studied using targeted approaches...
  91. ncbi SEC65 gene product is a subunit of the yeast signal recognition particle required for its integrity
    B C Hann
    Department of Biochemistry and Biophysics, University of California, Medical School, San Francisco 94143 0448
    Nature 356:532-3. 1992
    Protein targeting to the endoplasmic reticulum (ER) in mammalian cells is catalysed by the signal recognition particle (SRP), which consists of six protein subunits and an RNA subunit...
  92. ncbi SRP samples nascent chains for the presence of signal sequences by interacting with ribosomes at a discrete step during translation elongation
    S C Ogg
    Department of Biochemistry and Biophysics Medical School, University of California, San Francisco 94143 0448, USA
    Cell 81:1075-84. 1995
    The signal recognition particle (SRP) binds to ribosomes that synthesize nascent chains bearing signal sequences and catalyzes their targeting to the endoplasmic reticulum membrane. In S...
  93. pmc Lipid activation of the signal recognition particle receptor provides spatial coordination of protein targeting
    Vinh Q Lam
    Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, CA 91125, USA
    J Cell Biol 190:623-35. 2010
    The signal recognition particle (SRP) and SRP receptor comprise the major cellular machinery that mediates the cotranslational targeting of proteins to cellular membranes...
  94. pmc Protein SRP68 of human signal recognition particle: identification of the RNA and SRP72 binding domains
    Elena Iakhiaeva
    Department of Molecular Biology, University of Texas Health Science Center at Tyler, Tyler, Texas 75708 3154, USA
    Protein Sci 15:1290-302. 2006
    The signal recognition particle (SRP) plays an important role in the delivery of secretory proteins to cellular membranes...
  95. ncbi Recruitment of the earliest component of the bacterial flagellum to the old cell division pole by a membrane-associated signal recognition particle family GTP-binding protein
    Johnathan C D Green
    Department of Pathology, University of Cambridge, UK
    J Mol Biol 391:679-90. 2009
    The specialised signal recognition particle family guanosine 5c-triphosphate (GTP)-binding protein FlhF is required for the correct localisation of flagella in several bacterial species...
  96. ncbi Signal recognition particle mediated protein targeting in Escherichia coli
    Q A Valent
    Department of Molecular Microbiology, Institute of Molecular Biological Sciences, BioCentrum Amsterdam, Faculty of Biology, Vrije Universiteit Amsterdam, The Netherlands
    Antonie Van Leeuwenhoek 79:17-31. 2001
    The signal recognition particle (SRP) is a conserved ribonucleoprotein complex that binds to targeting sequences in nascent secretory and membrane proteins...
  97. ncbi Structure of SRP14 from the Schizosaccharomyces pombe signal recognition particle
    Mark A Brooks
    IBBMC CNRS UMR8619, Batiment 430, Universite de Paris Sud, Orsay, France
    Acta Crystallogr D Biol Crystallogr 65:421-33. 2009
    The signal recognition particle (SRP) Alu domain has been implicated in translation elongation arrest in yeasts and mammals. Fission yeast SRP RNA is similar to that of mammals, but has a minimal Alu-domain RNA lacking two stem-loops...
  98. ncbi Biogenesis of inner membrane proteins in Escherichia coli
    Joen Luirink
    Department of Microbiology, Institute of Molecular Cell Biology, Vrije Universiteit, 1081 HV Amsterdam, The Netherlands
    Annu Rev Microbiol 59:329-55. 2005
    ..Targeting involves a relatively simple signal recognition particle (SRP) and SRP-receptor...
  99. pmc Characterization of APOBEC3G binding to 7SL RNA
    Daniel Bach
    Global Health Institute, School of Life Sciences and Frontiers in Genetics, National Center for Competence in Research, Ecole Polytechnique Federale de Lausanne EPFL, CH 1015, Lausanne, Switzerland
    Retrovirology 5:54. 2008
    ..particles by RNA interference against SRP14 or by over expression of SRP19, both components of the signal recognition particle. We thus conclude that 7SL RNA is not an essential mediator of the virion packaging of these antiviral ..
  100. pmc Multiple conformational switches in a GTPase complex control co-translational protein targeting
    Xin Zhang
    Division of Chemistry and Chemical Engineering, California Institute of Technology, 1200 East California Boulevard, Pasadena, CA 91125, USA
    Proc Natl Acad Sci U S A 106:1754-9. 2009
    ..A notable exception to this paradigm is provided by two GTPases in the signal recognition particle (SRP) and the SRP receptor (SR) that control the co-translational targeting of proteins to cellular ..
  101. pmc Circular permutation as a tool to reduce surface entropy triggers crystallization of the signal recognition particle receptor beta subunit
    Thomas U Schwartz
    Howard Hughes Medical Institute, Laboratory of Cell Biology, The Rockefeller University, New York, New York 10021, USA
    Protein Sci 13:2814-8. 2004
    ..We encountered this problem during an attempt to crystallize the beta subunit of the eukaryotic signal recognition particle (SRbeta), bearing a long and flexible internal loop. Native SRbeta did not crystallize...

Research Grants58

  1. MEMBRANES OF THE DENTAL PATHOGEN STREPTOCOCCUS MUTANS
    L Jeannine Brady; Fiscal Year: 2013
    ..The signal recognition particle (SRP) pathway is a co-translational protein translocation pathway conserved in all living cells and ..
  2. Studies of YlxM of Streptococcus mutans, a newly identified component of the Sign
    MATTHEW LAWRENCE WILLIAMS; Fiscal Year: 2013
    ..The Signal Recognition Particle (SRP) pathway of Streptococcus mutans is known to play an important role in its membrane biogenesis and ..
  3. Membrane Protein Folding and Assembly
    Stephen H White; Fiscal Year: 2013
    ..TM helices along two different pathways: the SecA post-translational pathway or the co-translational signal recognition particle (SRP) pathway. This approach will clarify the relation between physical and biological principles...
  4. Roles of Streptococcus mutans YidC1 and YidC2 in membrane biogenesis
    SARA RASER PALMER; Fiscal Year: 2010
    ..We demonstrated in S. mutans the dispensability of the signal recognition particle (SRP) co-translational protein translocation pathway, previously believed to be essential in every ..
  5. Synapt G2-S System with HXMS Automation
    Elizabeth A Komives; Fiscal Year: 2013
    ..analyze the conformational changes in interleukins and kinases;Shan will map the substrate- and receptor- interactions in the chloroplast Signal Recognition Particle;and Kay will screen modulators of circadian rhythm-control proteins.
  6. mRNA Localization in Organelle Biogenesis
    Christopher V Nicchitta; Fiscal Year: 2013
    ..Thus, mRNAs encoding secretory/integral membrane proteins are localized to the ER via the Signal Recognition Particle (SRP) pathway and mRNAs encoding cytosolic/nucleoplasmic proteins, which lack encoded signal sequences, ..
  7. MECHANISM OF PROTEIN TRANSLOCATION ACROSS MEMBRANES
    Peter Walter; Fiscal Year: 2013
    ..proteins are targeted to the ER by an evolutionarily conserved molecular machine that consists of the signal recognition particle (SRP) and the SRP receptor (SR)...
  8. Molecular Basis of Tail-Anchored Membrane Protein Targeting
    Robert J Keenan; Fiscal Year: 2013
    ..For most membrane proteins, this is achieved co-translationally in a process mediated by the signal recognition particle. However, for the nearly 5% of all eukaryotic membrane proteins that are tail-anchored, targeting is ..
  9. Targeting of tail-anchored membrane proteins by the Get pathway.
    William M Clemons; Fiscal Year: 2013
    ..This constraint prevents them from following the canonical signal recognition particle dependent co-translational targeting pathway...
  10. Role of the TOC complex in plastid protein import
    Danny J Schnell; Fiscal Year: 2013
    ..The proposed roles of the TOC GTPases are reminiscent of the roles of the signal recognition particle (SRP/Ffh) and SRP receptor (SR/FtsY) in preprotein targeting to the ER and bacterial cytoplasmic ..
  11. Interplay between the Signal Recognition Particle and Trigger Factor at the Ribos
    Aileen R Ariosa; Fiscal Year: 2013
    ..of a translating ribosome with two ribosome-associated factors that ensure proper biogenesis: the signal recognition particle (SRP) and the trigger factor (TF)...
  12. SRP RNA level as a determinant of leishmanial parasitism of macrophages
    Gautam Chaudhuri; Fiscal Year: 2009
    ..are down regulated in macrophages upon exposure to Leishmania include 7SL RNA, the RNA component of the signal recognition particle (SRP)...
  13. Molecular Mechanism of Protein Targeting by the Signal Recognition Particle
    Shu ou Shan; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Proper localization of proteins is crucial for all cells. The signal recognition particle (SRP) and its receptor (SR) constitute the major cellular machinery that mediates the co-translational ..
  14. Monovalent metal ion binding sites in nucleic acids
    SCOTT STROBEL; Fiscal Year: 2004
    ..the role of two monovalent metal ion binding sites located within the protein-RNA core of the signal recognition particle, one of the most conserved ribonucleoprotein interactions in biology; (iv) Development of 205Tl NMR as ..
  15. NA,K-ATPASE--STRUCTURE, BIOSYNTHESIS, AND REGULATION
    LOWELL HOKIN; Fiscal Year: 1991
    ..This system will also be used to test for involvement of signal recognition particle. A brine shrimp genomic library will be constructed in order to examine the genomic structure of the ..
  16. GENETIC ANALYSIS TO THE SIGNAL RECOGNITION PARTICLE
    Gregory Phillips; Fiscal Year: 1999
    The signal recognition particle (SRP) is a ribonucleoprotein complex that is important for targeting exported proteins to the eukaryotic endoplasmic reticulum...
  17. AUTOANTIBODIES IN DERMATOMYOSITIS
    Ira Targoff; Fiscal Year: 1993
    ..the PM form, such as anti-Jo-1, antibodies to other aminoacyl-tRNA synthetases, and antibody to the signal recognition particle. DM antibodies are less well characterized, and only one antibody is predominantly associated with the ..
  18. ENDOPLASMIC RETICULUM OF TRYPANOSOMATIDS
    Kojo Mensa Wilmot; Fiscal Year: 2003
    ..Translocation of proteins into the ER of eukaryotes is dependent on an N-terminal signal sequence, signal recognition particle (SRP), SRP receptor (5R), and a translocon (formed by the 5ec61 p complex)...
  19. Signal Sequences -- Conformations and Membrane Binding
    LILA GIERASCH; Fiscal Year: 2006
    ..coli, that involving Ffh, which is homologous to the 54 kDa subunit of the mammalian signal recognition particle (SRP), and that involving SecA, a uniquely prokaryotic protein...
  20. The Mammalian Signal Recognition Particle
    KEVIN WEEKS; Fiscal Year: 2006
    unreadable] DESCRIPTION (provided by applicant): The signal recognition particle (SRP) binds the N-terminal polypeptide signal sequence emerging from a translating ribosome and targets this ribosomal complex to a receptor at the ..
  21. Biogenesis of Small RNAs
    SANDRA WOLIN; Fiscal Year: 2009
    ..RNAs, such as the spliceosomal U snRNAs, transfer RNAs, ribosomal RNAs and the RNA components of signal recognition particle and telomerase, play critical roles in eukaryotic cell metabolism...
  22. Functions of 4.5S RNA in the bacterial cell
    Gregory Phillips; Fiscal Year: 2007
    The signal recognition particle (SRP) is a highly evolutionarily conserved ribonucleoprotein complex that functions to target proteins into biological membranes. Because E...
  23. INTERDOMAIN COMMUNICATION IN E. COLI SRP
    Elena Falkovskaia; Fiscal Year: 2003
    ..Prokaryotic cell signal recognition particle (SRP) is composed of a protein component termed Ffh and 4.5S RNA...
  24. Mechanisms of mRNA localization in eukaryotic cells
    Brook Pyhtila; Fiscal Year: 2006
    ..and integral membrane proteins are selectively partitioned to the endoplasmic reticulum (ER) via the signal recognition particle (SRP) pathway. However, early studies suggest that mRNA binds to the ER in the absence of ribosomes...
  25. The function of a conserved cysteine pair in SRP54
    DOUGLAS FREYMANN; Fiscal Year: 2004
    The signal recognition particle (SRP) is an evolutionarily conserved ribonucleoprotein that targets 'nascent secreted and membrane proteins to the endoplasmic reticulum...
  26. High Resolution Structural Biology of the SRP GTPase
    DOUGLAS FREYMANN; Fiscal Year: 2005
    ..functional understanding of the molecular mechanisms of the two GTPases that play a central role in signal recognition particle (SRP) mediated targeting of secreted and membrane proteins...
  27. MECHANISM OF PROTEIN TRANSLOCATION ACROSS MEMBRANES
    Peter Walter; Fiscal Year: 2002
    ..Specifically, i) we will determine the molecular details of signal recognition particle (SRP) - signal sequence interactions and SRP-ribosome interactions and ask how these interactions are ..
  28. DISRUPTION OF EUBACTERIAL 4.5S RNA -P48 COMPLEX
    Richard Griffey; Fiscal Year: 1999
    The eubacterial signal recognition particle, comprised of a 4.5S RNA and protein P48, will be explored as a new class of target for rationally designed small-molecule antimicrobial compounds...
  29. Structure and function of SRP RNA
    Christian Zwieb; Fiscal Year: 2006
    ..Our particular focus is on the signal recognition particle (SRP) as it plays a central role in protein targeting and secretion...
  30. SIGNAL SEQUENCE RECOGNITION AND PROTEIN TARGETING
    Robert Stroud; Fiscal Year: 2007
    DESCRIPTION (provided by applicant): The goal of this, now amended, first renewal remains to understand Signal Recognition Particle (SRP) dependent targeting of membrane and secretory proteins to the translocation machinery of the cell, ..
  31. THYMIDYLATE SYNTHASE
    Robert Stroud; Fiscal Year: 2001
    ..Irwin Kunts, UCSF. Some mechanistic question will be approached using NMR spectroscopy in collaboration with Dr. Thomas James, UCSF. ..
  32. 2005 Mechanisms of Membrane Transport Gordon Conference
    Robert Stroud; Fiscal Year: 2005
    ..abstract_text> ..
  33. RNA Transport Within the Nucleus
    Thoru Pederson; Fiscal Year: 2005
    ..Another aspect of the project will address the intranuclear traffic of these RNAs in relation to proteins known to be involved in nuclear import or RNA export, i.e. the Ran-GTPase and TAP. ..
  34. STRUCTURE BASED DRUG DESIGN
    Robert Stroud; Fiscal Year: 2004
    ..Public domain access to the structures of therapeutic targets and technology will encourage their use by major pharmaceutical companies for the better design of therapeutics. ..
  35. Molecular Basis for Transmembrane Conduction & Signaling
    Robert Stroud; Fiscal Year: 2006
    ..A further aim is to improve crystals of the Acetylcholine receptor as a means of three-dimensional structure determination of an archetype of gating mechanisms in an archetype of neuroreceptor superfamilies. ..
  36. RNA PROCESSING AND RIBONUCLEOPROTEIN
    Thoru Pederson; Fiscal Year: 2001
    ..In Aim 2 the applicant's recent discovery that signal recognition particle (SRP) RNA is localized (at least transiently) in the nucleolus will be the basis for a search for ..
  37. Gravitaxic behavior in Drosophila
    KATHLEEN BECKINGHAM; Fiscal Year: 2003
    ..are used to sense gravity? iii) What role does light play in modulating gravitaxic responses? iv) What molecular signaling pathways are used in the processing of gravitational information in the peripheral and central nervous systems? ..
  38. POSTTRANSLATIONAL PROTEIN TRANSPORT INTO YEAST ER
    Tom Rapoport; Fiscal Year: 2003
    ..It will test the hypothesis that this protein works as a molecular ratchet rather than a machine that "pulls" the protein through the membrane. ..
  39. Inner Membrane Protein Assembly in Bacteria
    Ross Dalbey; Fiscal Year: 2009
    ..These studies should impact the field of bacterial membrane protein biogenesis and advance the knowledge of analogous pathways in eukaryotic cells. ..
  40. Biochemical and Structural Studies of RNA-Protein Complexes
    James R Williamson; Fiscal Year: 2010
    ..These studies will provide mechanistic and structural insights into common mechanisms for post-transcriptional gene regulation. ..
  41. MECHANISM OF DRUG BINDING TO P-GLYCOPROTEIN
    Jian Ting Zhang; Fiscal Year: 2001
    ..This work may lead us to the discovery of a new family of chaperone proteins associated with ribosomes that are important in determining the folding of membrane proteins co-translationally. ..
  42. ANALYSIS OF GOLGI MEMBRANE PROTEIN LOCALIZATION IN YEAST
    Steven Nothwehr; Fiscal Year: 2003
    ..Finally, several genes have been identified in genetic screens as defective in Golgi retention of proteins, and four of these will be investigated in further detail: grd20, grd21, grd22 and grd23. ..
  43. MOLECULAR BASES OF MULTIDRUG RESISTANCE IN CANCERS
    Jian Ting Zhang; Fiscal Year: 2001
    ..Additionally, this study will increase our understanding of how specific protein topological structures are related to transport functions in general. ..
  44. Targeting the oligomerization of human ABCG2
    Jian Ting Zhang; Fiscal Year: 2009
    ..This work may also lead us to the discovery of a new class of therapeutic agents that can help overcome drug-resistant cancers. ..
  45. Discovery of Novel RNA Genes in Genomic DNA Sequences
    STEPHEN HOLBROOK; Fiscal Year: 2005
    ....
  46. A Structural Classification of RNA
    STEPHEN HOLBROOK; Fiscal Year: 2005
    ..The database will also provide a convenient and logical means for experts and neophytes alike to access and interpret the growing wealth of RNA sequence and structural data. ..
  47. PROBABILISTIC MODELS OF PROTEIN AND RNA STRUCTURE
    Sean Eddy; Fiscal Year: 2006
    ..A final aim proposes a method for identifying the mRNA targets of regulatory RNAs (such as the newly discovered micro RNAs) by comparative genome analysis. ..
  48. Prion-Based Mechanisms in Metazoan Development
    KORIE HANDWERGER; Fiscal Year: 2006
    ..Finally, CePrDps will be examined for their ability to regulate development in the absence of their putative prion domains. ..
  49. Protein Transport Across Cell Membranes Gordon Conference 2005
    Ross Dalbey; Fiscal Year: 2005
    ..abstract_text> ..
  50. CALCIUM SIGNALING IN THE TESTIS
    KATHLEEN BECKINGHAM; Fiscal Year: 2004
    ..The Androcam homologs in other species will also be isolated with a particular view to pursuing studies of Androcam-related proteins in mammalian species. ..
  51. Assemply of the 30S Ribosomal Subunit
    James Williamson; Fiscal Year: 2007
    ..This will allow us to monitor the 30S assembly from a global perspective. [unreadable] [unreadable]..
  52. Isotopically Labeled Nucleotides for Biomolecular NMR
    James Williamson; Fiscal Year: 2007
    ..In order to make these reagents widely available to the structural biology community, we propose to optimize and scale up production of these reagents for commercial distribution. ..
  53. Genetic Analysis of the Escherichia coli Tat Pathway
    George Georgiou; Fiscal Year: 2007
    ..abstract_text> ..
  54. Role of p170 in lung tumorigenesis
    Jian Ting Zhang; Fiscal Year: 2007
    ..This work will certainly add to our understanding of the functional mechanism of translation initiation of protein synthesis. ..
  55. Targeting RNA Binding and Nuclear Export of HIV Rev
    James Williamson; Fiscal Year: 2007
    ..abstract_text> ..
  56. Interconversion of Specificity within Enzyme Families
    George Georgiou; Fiscal Year: 2008
    ..Finally, but perhaps equally importantly, this work will validate a unique, highly interdisciplinary approach for the exploration and deeper understanding of enzyme function. [unreadable] [unreadable]..
  57. HTS for Discovery of Inhibitors of STAR Protein-RNA complexes
    James Williamson; Fiscal Year: 2008
    ..Such validated inhibitors would be powerful tools for functional studies in C. elegans and mice, and the assay should be readily extended to inhibitors of Sam68 once suitable RNA targets have been identified. ..