heterogeneous nuclear ribonucleoproteins

Summary

Summary: A family of ribonucleoproteins that were originally found as proteins bound to nascent RNA transcripts in the form of ribonucleoprotein particles. Although considered ribonucleoproteins they are primarily classified by their protein component. They are involved in a variety of processes such as packaging of RNA and RNA TRANSPORT within the nucleus. A subset of heterogeneous-nuclear ribonucleoproteins are involved in additional functions such as nucleocytoplasmic transport (ACTIVE TRANSPORT, CELL NUCLEUS) of RNA and mRNA stability in the CYTOPLASM.

Top Publications

  1. ncbi An extended inhibitory context causes skipping of exon 7 of SMN2 in spinal muscular atrophy
    Natalia N Singh
    Department of Medicine, University of Massachusetts Medical School, Worcester, MA 01605 2324, USA
    Biochem Biophys Res Commun 315:381-8. 2004
  2. pmc The exosome associates cotranscriptionally with the nascent pre-mRNP through interactions with heterogeneous nuclear ribonucleoproteins
    Viktoria Hessle
    Department of Molecular Biology and Functional Genomics, Stockholm University, SE 10691 Stockholm, Sweden
    Mol Biol Cell 20:3459-70. 2009
  3. pmc Heterogeneous nuclear ribonucleoproteins (hnRNPs) in cellular processes: Focus on hnRNP E1's multifunctional regulatory roles
    Arindam Chaudhury
    Department of Cancer Biology, Lerner Research Institute, Cleveland Clinic, Cleveland, OH 44195, USA
    RNA 16:1449-62. 2010
  4. ncbi Exon identity established through differential antagonism between exonic splicing silencer-bound hnRNP A1 and enhancer-bound SR proteins
    J Zhu
    Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA
    Mol Cell 8:1351-61. 2001
  5. ncbi Determination of the RNA binding specificity of the heterogeneous nuclear ribonucleoprotein (hnRNP) H/H'/F/2H9 family
    M Caputi
    Department of Molecular, Cellular, and Developmental Biology and Center for Molecular Biology of RNA, Sinsheimer Laboratories, University of California, Santa Cruz, California 95064, USA
    J Biol Chem 276:43850-9. 2001
  6. pmc hnRNP H is a component of a splicing enhancer complex that activates a c-src alternative exon in neuronal cells
    M Y Chou
    Howard Hughes Medical Institute, University of California, Los Angeles, Los Angeles, California 90095, USA
    Mol Cell Biol 19:69-77. 1999
  7. ncbi Regulation of alternative pre-mRNA splicing by hnRNP A1 and splicing factor SF2
    A Mayeda
    Cold Spring Harbor Laboratory, New York, New York 11724 2208
    Cell 68:365-75. 1992
  8. pmc SMN interacts with a novel family of hnRNP and spliceosomal proteins
    Z Mourelatos
    Howard Hughes Medical Institute and Department of Biochemistry and Biophysics, University of Pennsylvania School of Medicine, Philadelphia, PA 19104 6148, USA
    EMBO J 20:5443-52. 2001
  9. pmc The hnRNP F protein: unique primary structure, nucleic acid-binding properties, and subcellular localization
    M J Matunis
    Howard Hughes Medical Institute, University of Pennsylvania School of Medicine, Philadelphia 19104 6148
    Nucleic Acids Res 22:1059-67. 1994
  10. ncbi Autoantibodies to heterogeneous nuclear ribonucleoproteins
    Roberto Caporali
    Chair and Division of Rheumatology University of Pavia IRCCS Policlinico S Matteo Pavia Italy
    Autoimmunity 38:25-32. 2005

Detail Information

Publications292 found, 100 shown here

  1. ncbi An extended inhibitory context causes skipping of exon 7 of SMN2 in spinal muscular atrophy
    Natalia N Singh
    Department of Medicine, University of Massachusetts Medical School, Worcester, MA 01605 2324, USA
    Biochem Biophys Res Commun 315:381-8. 2004
    ....
  2. pmc The exosome associates cotranscriptionally with the nascent pre-mRNP through interactions with heterogeneous nuclear ribonucleoproteins
    Viktoria Hessle
    Department of Molecular Biology and Functional Genomics, Stockholm University, SE 10691 Stockholm, Sweden
    Mol Biol Cell 20:3459-70. 2009
    ..Our results lead to a revised mechanistic model for cotranscriptional quality control in which the exosome is constantly recruited to newly synthesized RNAs through direct interactions with specific hnRNP proteins...
  3. pmc Heterogeneous nuclear ribonucleoproteins (hnRNPs) in cellular processes: Focus on hnRNP E1's multifunctional regulatory roles
    Arindam Chaudhury
    Department of Cancer Biology, Lerner Research Institute, Cleveland Clinic, Cleveland, OH 44195, USA
    RNA 16:1449-62. 2010
    b>Heterogeneous nuclear ribonucleoproteins (hnRNPs) comprise a family of RNA-binding proteins...
  4. ncbi Exon identity established through differential antagonism between exonic splicing silencer-bound hnRNP A1 and enhancer-bound SR proteins
    J Zhu
    Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA
    Mol Cell 8:1351-61. 2001
    ..The differential antagonism between a negative and two positive regulators exemplifies how inclusion of an alternative exon can be modulated...
  5. ncbi Determination of the RNA binding specificity of the heterogeneous nuclear ribonucleoprotein (hnRNP) H/H'/F/2H9 family
    M Caputi
    Department of Molecular, Cellular, and Developmental Biology and Center for Molecular Biology of RNA, Sinsheimer Laboratories, University of California, Santa Cruz, California 95064, USA
    J Biol Chem 276:43850-9. 2001
    ..Mutation of the C to an A changes the specificity of the DCS from a substrate that binds only hnRNP H/H' to a binding site for all hnRNP H family members. We conclude that the sequence GGGA is recognized by all hnRNP H family proteins...
  6. pmc hnRNP H is a component of a splicing enhancer complex that activates a c-src alternative exon in neuronal cells
    M Y Chou
    Howard Hughes Medical Institute, University of California, Los Angeles, Los Angeles, California 90095, USA
    Mol Cell Biol 19:69-77. 1999
    ..These two proteins presumably cooperate with each other and with other enhancer complex proteins to direct splicing to the N1 exon upstream...
  7. ncbi Regulation of alternative pre-mRNA splicing by hnRNP A1 and splicing factor SF2
    A Mayeda
    Cold Spring Harbor Laboratory, New York, New York 11724 2208
    Cell 68:365-75. 1992
    ..The regulation of these antagonistic activities may play an important role in the tissue-specific and developmental control of gene expression by alternative splicing...
  8. pmc SMN interacts with a novel family of hnRNP and spliceosomal proteins
    Z Mourelatos
    Howard Hughes Medical Institute and Department of Biochemistry and Biophysics, University of Pennsylvania School of Medicine, Philadelphia, PA 19104 6148, USA
    EMBO J 20:5443-52. 2001
    ..We further demonstrate that hnRNP Qs are required for efficient pre-mRNA splicing in vitro. The hnRNP Q proteins may provide a molecular link between the SMN complex and splicing...
  9. pmc The hnRNP F protein: unique primary structure, nucleic acid-binding properties, and subcellular localization
    M J Matunis
    Howard Hughes Medical Institute, University of Pennsylvania School of Medicine, Philadelphia 19104 6148
    Nucleic Acids Res 22:1059-67. 1994
    More than 20 different heterogeneous nuclear ribonucleoproteins (hnRNPs) are associated with pre-mRNAs in the nucleus of mammalian cells and these proteins appear to influence pre-mRNA processing and other aspects of mRNA metabolism and ..
  10. ncbi Autoantibodies to heterogeneous nuclear ribonucleoproteins
    Roberto Caporali
    Chair and Division of Rheumatology University of Pavia IRCCS Policlinico S Matteo Pavia Italy
    Autoimmunity 38:25-32. 2005
    b>Heterogeneous nuclear ribonucleoproteins (hnRNPs) are among the most abundant proteins in the eukaryotic cell nucleus and play a direct role in several aspects of the RNA life including splicing, export of the mature RNAs and translation...
  11. pmc SR proteins and hnRNP H regulate the splicing of the HIV-1 tev-specific exon 6D
    Massimo Caputi
    Department of Molecular, Cell and Developmental Biology, Sinsheimer Laboratory, University of California Santa Cruz, Santa Cruz, CA 95064, USA
    EMBO J 21:845-55. 2002
    ..hnRNP H is required for interaction of U1 snRNP with the enhancer, independent of the point mutation. We propose that SC35 binding to the point mutation region may convert the hnRNP H-U1 snRNP complex into a splicing enhancer...
  12. pmc The poly(C)-binding proteins: a multiplicity of functions and a search for mechanisms
    Aleksandr V Makeyev
    Department of Genetics, University of Pennsylvania School of Medicine, Philadelphia 19104 6148, USA
    RNA 8:265-78. 2002
    ..Clearly the identification of additional binding targets and delineation of corresponding control mechanisms and effector pathways will establish highly informative models for further exploration...
  13. ncbi Vimentin interacts with heterogeneous nuclear ribonucleoproteins and dengue nonstructural protein 1 and is important for viral replication and release
    Rattiyaporn Kanlaya
    Medical Proteomics Unit, Office for Research and Development, Faculty of Medicine Siriraj Hospital, Mahidol University, 12th Floor Adulyadejvikrom Building, 2 Prannok Road, Bangkoknoi, Bangkok 10700, Thailand
    Mol Biosyst 6:795-806. 2010
    ..study using expression proteomics demonstrated that many proteins, particularly five forms of heterogeneous nuclear ribonucleoproteins (hnRNPs), were up-regulated in human endothelial cells upon dengue virus infection...
  14. ncbi Alternative splicing: multiple control mechanisms and involvement in human disease
    Javier F Caceres
    MRC Human Genetics Unit, Western General Hospital, Edinburgh EH4 2XU, UK
    Trends Genet 18:186-93. 2002
    ..We also focus on the role of the transcriptional machinery in the regulation of alternative splicing, and on those alterations of alternative splicing that lead to human disease...
  15. pmc RNA splicing at human immunodeficiency virus type 1 3' splice site A2 is regulated by binding of hnRNP A/B proteins to an exonic splicing silencer element
    P S Bilodeau
    Department of Microbiology, University of Iowa, Iowa City, Iowa 52242, USA
    J Virol 75:8487-97. 2001
    ..The results suggest that coordinate repression of HIV-1 RNA splicing is mediated by members of the hnRNP A/B protein family...
  16. ncbi hnRNP-G promotes exon 7 inclusion of survival motor neuron (SMN) via direct interaction with Htra2-beta1
    Yvonne Hofmann
    Institute of Human Genetics, University of Bonn, Bonn, Germany
    Hum Mol Genet 11:2037-49. 2002
    ..Finally, we suggest a model of how the exon 7 mRNA processing is regulated by the splicing factors identified so far...
  17. pmc Dual requirement for yeast hnRNP Nab2p in mRNA poly(A) tail length control and nuclear export
    Ronald E Hector
    Department of Molecular Genetics and Microbiology, Powell Gene Therapy Center, University of Florida College of Medicine, Gainesville, FL 32610 0266, USA
    EMBO J 21:1800-10. 2002
    ..We propose that Nab2p provides an important link between the termination of mRNA polyadenylation and nuclear export...
  18. ncbi A nuclear export signal in hnRNP A1: a signal-mediated, temperature-dependent nuclear protein export pathway
    W M Michael
    Howard Hughes Medical Institute, University of Pennsylvania School of Medicine, Philadelphia 19104 6148, USA
    Cell 83:415-22. 1995
    ..These findings demonstrate that there is a signal-dependent, temperature-sensitive nuclear export pathway and strengthen the suggestion that A1 and other shuttling hnRNPs function as carriers for RNA during export to the cytoplasm...
  19. ncbi Omega speckles - a novel class of nuclear speckles containing hnRNPs associated with noncoding hsr-omega RNA in Drosophila
    K V Prasanth
    Cytogenetics Laboratory, Department of Zoology, Banaras Hindu University, Varanasi 221 005, India
    J Cell Sci 113:3485-97. 2000
    ....
  20. pmc Sumoylation of heterogeneous nuclear ribonucleoproteins, zinc finger proteins, and nuclear pore complex proteins: a proteomic analysis
    Tianwei Li
    Laboratory of Biochemistry, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892 8012, USA
    Proc Natl Acad Sci U S A 101:8551-6. 2004
    ..Results reveal that several heterogeneous nuclear ribonucleoproteins (hnRNPs), zinc finger proteins, and nuclear pore complex proteins were sumoylated...
  21. ncbi A model for heterogeneous nuclear ribonucleoproteins in telomere and telomerase regulation
    Lance P Ford
    Ambion, Inc, 2130 Woodward Street, Austin, Texas, TX 78744 1832, USA
    Oncogene 21:580-3. 2002
    The heterogeneous nuclear ribonucleoproteins (hnRNPs) are a large family of nucleic acid binding proteins that are often found in, but not restricted to, the 40S-ribonucleoprotein particle...
  22. pmc Downstream sequence elements with different affinities for the hnRNP H/H' protein influence the processing efficiency of mammalian polyadenylation signals
    George K Arhin
    Department of Microbiology and Molecular Genetics, UMDNJ New Jersey Medical School, 185 South Orange Avenue, Newark, NJ 07103, USA
    Nucleic Acids Res 30:1842-50. 2002
    ..Sequences capable of binding hnRNP H protein with varying affinities may play a role in determining the processing efficiency of a significant number of mammalian polyadenylation signals...
  23. ncbi Suppression of 15-lipoxygenase synthesis by hnRNP E1 is dependent on repetitive nature of LOX mRNA 3'-UTR control element DICE
    Iris Reimann
    Biomedizinisches Forschungszentrum BMFZ University Clinics Charité, Augustenburger Platz 1, Humboldt University Berlin, D 13353, Germany
    J Mol Biol 315:965-74. 2002
    ..Most DICEs were two- to fourfold repetitive, but also highly repetitive structures were found, as in quail myelin protein mRNA (31 repeats) and hyperglycemic hormone mRNA of two crayfish species (nine and 11 repeats)...
  24. ncbi ERK phosphorylation drives cytoplasmic accumulation of hnRNP-K and inhibition of mRNA translation
    H Habelhah
    Ruttenberg Cancer Center, Mount Sinai School of Medicine, New York, New York 10029, USA
    Nat Cell Biol 3:325-30. 2001
    ..Our results establish the role of MAPK/ERK in phosphorylation-dependent cellular localization of hnRNP-K, which is required for its ability to silence mRNA translation...
  25. ncbi Messenger-RNA-binding proteins and the messages they carry
    Gideon Dreyfuss
    Howard Hughes Medical Institute and Department of Biochemistry and Biophysics, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104 6148, USA
    Nat Rev Mol Cell Biol 3:195-205. 2002
    ..These proteins communicate crucial information to the translation machinery for the surveillance of nonsense mutations and for mRNA localization and translation...
  26. pmc Identification of two KH domain proteins in the alpha-globin mRNP stability complex
    M Kiledjian
    Howard Hughes Medical Institute, Department of Genetics, Philadelphia 19104 6145, USA
    EMBO J 14:4357-64. 1995
    ..As such this represents the first example of a specific function for this class of proteins and suggests potential roles for other members of this protein family...
  27. ncbi An evolutionarily conserved germ cell-specific hnRNP is encoded by a retrotransposed gene
    D J Elliott
    MRC Human Genetics Unit, Western General Hospital, Crewe Road, Edinburgh EH4 2XU, UK
    Hum Mol Genet 9:2117-24. 2000
    ..The existence of an additional testis-specific hnRNP G family member provides evidence for the importance of these proteins in normal germ cell development...
  28. pmc Nucleocytoplasmic shuttling of polypyrimidine tract-binding protein is uncoupled from RNA export
    R V Kamath
    Department of Cell and Molecular Biology, Northwestern University Medical School, Chicago, Illinois 60611, USA
    Mol Biol Cell 12:3808-20. 2001
    ..The uncoupling of PTB shuttling and RNA export suggests that the nucleocytoplasmic shuttling of PTB may also play a regulatory role for its functions in the nucleus and cytoplasm...
  29. pmc SUMO modification of heterogeneous nuclear ribonucleoproteins
    Maria T Vassileva
    Department of Biochemistry and Molecular Biology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, Maryland 21205, USA
    Mol Cell Biol 24:3623-32. 2004
    ..Among the most abundant cargos transported through NPCs are the heterogeneous nuclear ribonucleoproteins (hnRNPs)...
  30. ncbi The roles of heterogeneous nuclear ribonucleoproteins (hnRNP) in RNA metabolism
    F Weighardt
    Istituto di Genetica Biochimica ed Evoluzionistica del CNR, Pavia, Italy
    Bioessays 18:747-56. 1996
    ..moiety of these complexes mainly constitutes a class of about 20 major polypeptides called heterogeneous nuclear ribonucleoproteins or hnRNPs...
  31. ncbi Hrb27C, Sqd and Otu cooperatively regulate gurken RNA localization and mediate nurse cell chromosome dispersion in Drosophila oogenesis
    Jennifer S Goodrich
    Howard Hughes Medical Institute, Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA
    Development 131:1949-58. 2004
    b>Heterogeneous nuclear ribonucleoproteins, hnRNPs, are RNA-binding proteins that play crucial roles in controlling gene expression...
  32. ncbi hnRNP A1 and the SR proteins ASF/SF2 and SC35 have antagonistic functions in splicing of beta-tropomyosin exon 6B
    Alain Expert-Bezançon
    Centre de Genetique Moleculaire, CNRS UPR 2167, Laboratoire propre associe a l Universite Pierre et Marie Curie, 91198 Gif sur Yvette, France
    J Biol Chem 279:38249-59. 2004
    ..These data indicate that the G-rich sequence is a composite element that acts as an enhancer or as a silencer, depending on which proteins bind to them...
  33. pmc The C-terminal domain of TAP interacts with the nuclear pore complex and promotes export of specific CTE-bearing RNA substrates
    A Bachi
    European Molecular Biology Laboratory, Heidelberg, Germany
    RNA 6:136-58. 2000
    ....
  34. pmc The C-protein tetramer binds 230 to 240 nucleotides of pre-mRNA and nucleates the assembly of 40S heterogeneous nuclear ribonucleoprotein particles
    M Huang
    Department of Molecular Biology, Vanderbilt University, Nashville, Tennessee 37235
    Mol Cell Biol 14:518-33. 1994
    ..These findings also demonstrate that unless excluded by other factors, the C proteins are likely to be located along the length of nascent transcripts...
  35. ncbi hnRNP proteins and splicing control
    Rebeca Martinez-Contreras
    Departement de Microbiologie et d Infectiologie, Universite de Sherbrooke, Quebec, Canada
    Adv Exp Med Biol 623:123-47. 2007
    ..Thus, hnRNP proteins utilize a variety of strategies to control splice site selection in a manner that is important for both alternative and constitutive pre-mRNA splicing...
  36. pmc Crystal structure of the two-RRM domain of hnRNP A1 (UP1) complexed with single-stranded telomeric DNA
    J Ding
    W M Keck Structural Biology Laboratory, Cold Spring Harbor, New York 11724, USA
    Genes Dev 13:1102-15. 1999
    ..Models for regular packaging of telomere 3' overhangs and for juxtaposition of alternative 5' splice sites are proposed...
  37. ncbi Distinct sets of adjacent heterogeneous nuclear ribonucleoprotein (hnRNP) A1/A2 binding sites control 5' splice site selection in the hnRNP A1 mRNA precursor
    Stephen Hutchison
    Departement de Microbiologie et d Infectiologie, Faculte de Medecine, Universite de Sherbrooke, Sherbrooke, Quebec J1H 5N4, Canada
    J Biol Chem 277:29745-52. 2002
    ..The presence of multiple A1/A2 binding sites downstream of common exon 7 and alternative exon 7B probably plays an important role in maximizing the activity of hnRNP A1/A2 proteins...
  38. pmc DSEF-1 is a member of the hnRNP H family of RNA-binding proteins and stimulates pre-mRNA cleavage and polyadenylation in vitro
    P S Bagga
    UMDNJ New Jersey Medical School, Department of Microbiology and Molecular Genetics, 185 South Orange Avenue, Newark, NJ 07103, USA
    Nucleic Acids Res 26:5343-50. 1998
    ..These observations suggest that DSEF-1 is an auxiliary factor that assists in the assembly of the general 3'-end processing factors onto the core elements of the polyadenylation signal...
  39. pmc hnRNP F influences binding of a 64-kilodalton subunit of cleavage stimulation factor to mRNA precursors in mouse B cells
    K L Veraldi
    Department of Molecular Genetics and Biochemistry, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15261, USA
    Mol Cell Biol 21:1228-38. 2001
    ....
  40. pmc Binding of hnRNP H to an exonic splicing silencer is involved in the regulation of alternative splicing of the rat beta-tropomyosin gene
    C D Chen
    Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA
    Genes Dev 13:593-606. 1999
    ..The involvement of hnRNP H in the activity of an ESS may represent a prototype for the regulation of tissue- and developmental-specific alternative splicing...
  41. pmc c-Src-mediated phosphorylation of hnRNP K drives translational activation of specifically silenced mRNAs
    Antje Ostareck-Lederer
    European Molecular Biology Laboratory, Heidelberg, Germany
    Mol Cell Biol 22:4535-43. 2002
    ..Our results establish a novel role of c-Src kinase in translational gene regulation and reveal a mechanism by which silenced mRNAs can be translationally activated...
  42. ncbi Specific interaction of Smn, the spinal muscular atrophy determining gene product, with hnRNP-R and gry-rbp/hnRNP-Q: a role for Smn in RNA processing in motor axons?
    Wilfried Rossoll
    Institut für Klinische Neurobiologie, Department of Neurology, University of Wurzburg, Josef Schneider Strasse 11, D 97080 Wurzburg, Germany
    Hum Mol Genet 11:93-105. 2002
    ..Interestingly, hnRNP-R is predominantly located in axons of motor neurons and co-localizes with Smn in this cellular compartment. Thus, this finding could provide a key to understand a motor neuron-specific Smn function in SMA...
  43. ncbi Cloning of human 2H9 heterogeneous nuclear ribonucleoproteins. Relation with splicing and early heat shock-induced splicing arrest
    D Mahe
    Institut de Genetique et de Biologie Moleculaire et Cellulaire, CNRS INSERM ULP, BP 163, 67404 Illkirch Cedex, C U de Strasbourg, France
    J Biol Chem 272:1827-36. 1997
    Using antibody 2H9 from our heterogeneous nuclear ribonucleoproteins (anti-hnRNP) monoclonal antibody library, we previously showed in HeLa cells that a 35-37-kDa protein doublet switches from the hnRNP complexes to the nuclear matrix ..
  44. ncbi The roles of heterogeneous nuclear ribonucleoproteins in tumour development and progression
    Brian Carpenter
    Department of Pathology, University of Aberdeen, Foresterhill, Aberdeen AB25 2ZD, UK
    Biochim Biophys Acta 1765:85-100. 2006
    The heterogeneous nuclear ribonucleoproteins (hnRNP) are a family of proteins which share common structural domains, and extensive research has shown that they have central roles in DNA repair, telomere biogenesis, cell signaling and in ..
  45. ncbi Identification of GRY-RBP as an apolipoprotein B RNA-binding protein that interacts with both apobec-1 and apobec-1 complementation factor to modulate C to U editing
    V Blanc
    Department of Internal Medicine and Department of Pharmacology and Molecular Biology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 276:10272-83. 2001
    ..Taken together, the results indicate that GRY-RBP is a member of the ACF gene family that may function to modulate C to U RNA editing through binding either to ACF or to apobec-1 or, alternatively, to the target RNA itself...
  46. ncbi A mechanism for translationally coupled mRNA turnover: interaction between the poly(A) tail and a c-fos RNA coding determinant via a protein complex
    C Grosset
    Department of Biochemistry and Molecular Biology, The University of Texas Houston Medical School 77030, USA
    Cell 103:29-40. 2000
    ..We propose that a bridging complex forms between the poly(A) tail and the mCRD and ribosome transit disrupts or reorganizes the complex, leading to rapid RNA deadenylation and decay...
  47. ncbi Assignment of human KH-box-containing genes by in situ hybridization: HNRNPK maps to 9q21.32-q21.33, PCBP1 to 2p12-p13, and PCBP2 to 12q13.12-q13.13, distal to FRA12A
    N Tommerup
    Danish Center for Human Genome Research, The John F Kennedy Institute, Glostrup, Denmark
    Genomics 32:297-8. 1996
  48. ncbi SC35 and heterogeneous nuclear ribonucleoprotein A/B proteins bind to a juxtaposed exonic splicing enhancer/exonic splicing silencer element to regulate HIV-1 tat exon 2 splicing
    Alan M Zahler
    Department of Molecular, Cellular and Developmental Biology and Center for Molecular Biology of RNA, Sinsheimer Laboratories, University of California, Santa Cruz 95064, USA
    J Biol Chem 279:10077-84. 2004
    ..We show that the SC35 and the hnRNP A1 binding sites overlap within the juxtaposed ESE/ESS. We propose that hnRNP A1 binding to the ESS inhibits splicing of the upstream intron by directly masking the SC35 binding site...
  49. pmc Primary structures of the heterogeneous nuclear ribonucleoprotein A2, B1, and C2 proteins: a diversity of RNA binding proteins is generated by small peptide inserts
    C G Burd
    Department of Biochemistry, Molecular Biology, and Cell Biology, Northwestern University, Evanston, IL 60208
    Proc Natl Acad Sci U S A 86:9788-92. 1989
    ..These findings indicate that the addition of small peptides, probably by alternative pre-mRNA splicing, generates some of the diversity apparent among hnRNP proteins...
  50. ncbi A conserved mRNA export machinery coupled to pre-mRNA splicing
    Robin Reed
    Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
    Cell 108:523-31. 2002
    ..Export of mRNAs is coupled to upstream steps in gene expression, such as pre-mRNA splicing, and to downstream events, including nonsense-mediated decay...
  51. ncbi Identification of a sequence-specific single-stranded DNA binding protein that suppresses transcription of the mouse myelin basic protein gene
    S Haas
    Jefferson Institute of Molecular Medicine, Department of Biochemistry and Molecular Biology, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
    J Biol Chem 270:12503-10. 1995
    ..The myef-2 gene maps to mouse chromosome 2. The relevance of these findings for regulation of MBP gene expression and oligodendrocyte differentiation is discussed...
  52. ncbi Significantly increased antibody response to heterogeneous nuclear ribonucleoproteins in cerebrospinal fluid of multiple sclerosis patients but not in patients with human T-lymphotropic virus type I-associated myelopathy/tropical spastic paraparesis
    Motohiro Yukitake
    Department of Internal Medicine, Faculty of Medicine, Saga University, Saga, Japan
    J Neurovirol 14:130-5. 2008
    It has been reported that antibodies (Abs) against heterogeneous nuclear ribonucleoproteins (hnRNPs) are associated with human T-lymphotropic virus type I (HTLV-I)-associated myelopathy/tropical spastic paraparesis (HAM/TSP) and multiple ..
  53. pmc A novel set of nuclear localization signals determine distributions of the alphaCP RNA-binding proteins
    Alexander N Chkheidze
    Departments of Genetics and Medicine, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104, USA
    Mol Cell Biol 23:8405-15. 2003
    ..This complexity of alphaCP distribution is likely to contribute to the diverse functions mediated by this group of abundant RNA-binding proteins...
  54. ncbi Expression of poly(C)-binding proteins is differentially regulated by hypoxia and ischemia in cortical neurons
    Y Zhu
    Buck Institute for Age Research, 8001 Redwood Boulevard, Novato, CA 94945 0638, USA
    Neuroscience 110:191-8. 2002
    ..Since we observed no poly(C)-binding protein expression in astroglia, alternative mRNA stability mechanisms may exist in these cells...
  55. pmc The KH-domain protein alpha CP has a direct role in mRNA stabilization independent of its cognate binding site
    Jian Kong
    Department of Genetics, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    Mol Cell Biol 23:1125-34. 2003
    ..Once bound, alpha CP appears to be fully sufficient to trigger downstream events in the stabilization pathway...
  56. ncbi Isolation and identification of heterogeneous nuclear ribonucleoproteins (hnRNP) from purified plasma membranes of human tumour cell lines as albumin-binding proteins
    Thomas Fritzsche
    Division of Molecular Toxicology, German Cancer Research Center, Im Neuenheimer Feld 280, D 69120 Heidelberg, Germany
    Biochem Pharmacol 67:655-65. 2004
    ..MV3 and MCF7) by albumin affinity chromatography and identified them as four members of the heterogeneous nuclear ribonucleoproteins (hnRNP) family and calreticulin by matrix-assisted laser desorption ionisation time-of-flight ..
  57. ncbi Identification of human common nuclear-matrix proteins as heterogeneous nuclear ribonucleoproteins H and H' by sequencing and mass spectrometry
    K Holzmann
    Institute of Tumor Biology Cancer Research, University of Vienna, Austria
    Eur J Biochem 244:479-86. 1997
    ..The two human common nuclear-matrix proteins were identified as heterogeneous nuclear ribonucleoproteins (hnRNP) H and H' or their variants...
  58. pmc Signaling-dependent and coordinated regulation of transcription, splicing, and translation resides in a single coregulator, PCBP1
    Qingchang Meng
    Molecular and Cellular Oncology, University of Texas M D Anderson Cancer Center, Houston, TX 77030, USA
    Proc Natl Acad Sci U S A 104:5866-71. 2007
    ..These findings establish the principle that a single coregulator can function as a signal-dependent and coordinated regulator of transcription, splicing, and translation...
  59. ncbi Pre-mRNA splicing imprints mRNA in the nucleus with a novel RNA-binding protein that persists in the cytoplasm
    N Kataoka
    Howard Hughes Medical Institute and Department of Biochemistry and Biophysics, University of Pennsylvania School of Medicine, Philadelphia 19104, USA
    Mol Cell 6:673-82. 2000
    ..These findings suggest that pre-mRNA splicing imprints mRNA with a unique set of proteins that persists in the cytoplasm and thereby communicates the history of the transcript...
  60. pmc An mRNA stability complex functions with poly(A)-binding protein to stabilize mRNA in vitro
    Z Wang
    Department of Cell Biology and Neuroscience, Rutgers University, Piscataway, New Jersey 08854 8082, USA
    Mol Cell Biol 19:4552-60. 1999
    ..Therefore, the alpha-complex is an mRNA stability complex in vitro which could function at least in part by interacting with PABP...
  61. pmc Selection of alternative 5' splice sites: role of U1 snRNP and models for the antagonistic effects of SF2/ASF and hnRNP A1
    I C Eperon
    Department of Biochemistry, University of Leicester, Leicester LE1 7RH, United Kingdom
    Mol Cell Biol 20:8303-18. 2000
    ....
  62. pmc DNA-dependent protein kinase (DNA-PK) phosphorylates nuclear DNA helicase II/RNA helicase A and hnRNP proteins in an RNA-dependent manner
    Suisheng Zhang
    Department of Biochemistry, Institute of Molecular Biotechnology, Postfach 100 813, D 07708 Jena, Germany
    Nucleic Acids Res 32:1-10. 2004
    ..These results support the view that DNA-PK can also function as an RNA-dependent protein kinase to regulate some aspects of RNA metabolism, such as RNA processing and transport...
  63. ncbi Structure of PTB bound to RNA: specific binding and implications for splicing regulation
    Florian C Oberstrass
    Institute for Molecular Biology and Biophysics, Department of Biology, Swiss Federal Institute of Technology, Zurich, ETH Honggerberg, CH 8093 Zurich, Switzerland
    Science 309:2054-7. 2005
    ..Thus, PTB will induce RNA looping when bound to two separated pyrimidine tracts within the same RNA. This leads to structural models for how PTB functions as an alternative-splicing repressor...
  64. pmc Identification of a (CUG)n triplet repeat RNA-binding protein and its expression in myotonic dystrophy
    L T Timchenko
    Department of Medicine, Baylor College of Medicine, Houston, TX 77030, USA
    Nucleic Acids Res 24:4407-14. 1996
    ..We propose that the (CUG)n repeat region in Mt-PK mRNA is a binding site for CUG-BP/hNab50 in vivo, and triplet repeat expansion leads to sequestration of this hnRNP on mutant Mt-PK transcripts...
  65. pmc A novel nuclear structure containing the survival of motor neurons protein
    Q Liu
    Howard Hughes Medical Institute, University of Pennsylvania School of Medicine, Philadelphia 19104 6148, USA
    EMBO J 15:3555-65. 1996
    ..These structures are similar in number (2-6) and size (0.1-1.0 micron) to coiled bodies, and frequently are found near or associated with coiled bodies. We term these prominent nuclear structures gems, for Gemini of coiled bodies...
  66. ncbi BCR-ABL suppresses C/EBPalpha expression through inhibitory action of hnRNP E2
    Danilo Perrotti
    Department of Microbiology and Immunology, Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
    Nat Genet 30:48-58. 2002
    ..Our results indicate that BCR-ABL regulates the expression of C/EBPalpha by inducing hnRNP E2-which inhibits the translation of CEBPA mRNA...
  67. ncbi The UV-inducible RNA-binding protein A18 (A18 hnRNP) plays a protective role in the genotoxic stress response
    C Yang
    Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, 108 N Greene St, Baltimore, MD 21201 1503, USA
    J Biol Chem 276:47277-84. 2001
    ..These data suggest that A18 hnRNP plays a protective role against genotoxic stresses by translocating to the cytosol and stabilizing specific transcripts involved in cell survival...
  68. pmc P68 RNA helicase (DDX5) alters activity of cis- and trans-acting factors of the alternative splicing of H-Ras
    Maria Camats
    Unidad de Splicing, Instituto de Investigaciones Biomédicas de Barcelona Consejo Superior de Investigaciones Científicas, Barcelona, Spain
    PLoS ONE 3:e2926. 2008
    ..p68 RNA helicase (p68) is known to reduce IDX inclusion...
  69. ncbi Protein-protein interaction among hnRNPs shuttling between nucleus and cytoplasm
    J H Kim
    Department of Life Science, Pohang University of Science and Technology, Hyoja Dong San31, Pohang, Kyungbuk, 790 784, Korea
    J Mol Biol 298:395-405. 2000
    b>Heterogeneous nuclear ribonucleoproteins (hnRNPs) are involved in several RNA-related biological processes such as transcription, pre-mRNA processing, mature mRNA transport to the cytoplasm, and translation...
  70. pmc A nuclear localization domain in the hnRNP A1 protein
    H Siomi
    Howard Hughes Medical Institute, University of Pennsylvania School of Medicine, Philadelphia 19104 6148, USA
    J Cell Biol 129:551-60. 1995
    ..M9 is a novel type of nuclear localization domain as it does not contain sequences similar to classical basic-type NLS. Interestingly, sequences similar to M9 are found in other nuclear RNA-binding proteins including hnRNP A2...
  71. pmc Functional characterization of SR and SR-related genes in Caenorhabditis elegans
    D Longman
    MRC Human Genetics Unit, Western General Hospital, Edinburgh EH4 2XU
    EMBO J 19:1625-37. 2000
    ..RNAi with CeSRPK, an SR protein kinase, resulted in early embryonic lethality, suggesting an essential role for SR protein phosphorylation during development...
  72. ncbi A set of highly conserved RNA-binding proteins, alphaCP-1 and alphaCP-2, implicated in mRNA stabilization, are coexpressed from an intronless gene and its intron-containing paralog
    A V Makeyev
    Howard Hughes Medical Institute and the Departments of Genetics and Medicine, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104, USA
    J Biol Chem 274:24849-57. 1999
    ....
  73. ncbi Nuclear mRNA binding proteins couple pre-mRNA splicing and post-splicing events
    V N Kim
    Howard Hughes Medical Institute and Department of Biochemistry and Biophysics, University of Pennsylvania School of Medicine, Philadelphia 19104-6148, USA
    Mol Cells 12:1-10. 2001
    ..The detailed mechanism of coupling and the factors that mediate these processes remain to be determined in the coming years...
  74. pmc Heterogeneous nuclear ribonucleoprotein (hnRNP) E1 binds to hnRNP A2 and inhibits translation of A2 response element mRNAs
    Linda D Kosturko
    Department of Neuroscience, Molecular, Microbial, and Structural Biology, and Biomedical Science Graduate Program, University of Connecticut Health Center, Farmington, CT 06030, USA
    Mol Biol Cell 17:3521-33. 2006
    ..These results are consistent with a model where hnRNP E1 recruited to A2RE RNA granules by binding to hnRNP A2 inhibits translation of A2RE RNA during granule transport...
  75. pmc Interaction of the RNA-binding domain of the hnRNP C proteins with RNA
    M Görlach
    Howard Hughes Medical Institute, University of Pennsylvania School of Medicine, Philadelphia 19104 6148
    EMBO J 11:3289-95. 1992
    ..These observations localize the candidate residues of the RBD that are involved in the interaction with the RNA.(ABSTRACT TRUNCATED AT 250 WORDS)..
  76. pmc Identification of AUF1 (heterogeneous nuclear ribonucleoprotein D) as a component of the alpha-globin mRNA stability complex
    M Kiledjian
    Department of Cell, Developmental and Neurobiology, Rutgers University, Piscataway, New Jersey 08855, USA
    Mol Cell Biol 17:4870-6. 1997
    ..AUF1/hnRNP-D could, therefore, be a general mRNA turnover factor involved in both stabilization and decay of mRNA...
  77. pmc Heterogeneous nuclear ribonucleoprotein K is a transcription factor
    E F Michelotti
    Laboratory of Pathology, National Cancer Institute, National Institute of Health, Bethesda, Maryland 20892, USA
    Mol Cell Biol 16:2350-60. 1996
    ..These data indicate that hnRNP K binds to a specific cis element, interacts with the RNA polymerase II transcription machinery, and stimulates transcription and thus has all of the properties of a transcription factor...
  78. pmc Drosophila snRNP associated protein P11 which specifically binds to heat shock puff 93D reveals strong homology with hnRNP core protein A1
    B T Hovemann
    Zentrum für Molekulare Biologie Heidelberg, FRG
    Nucleic Acids Res 19:4909-14. 1991
    ..The P11 sequence corresponds to the Hrb87F sequence that was recently described by Haynes et al. (1) as hnRNP A related gene...
  79. ncbi HnRNP G and Tra2beta: opposite effects on splicing matched by antagonism in RNA binding
    M Talat Nasim
    Department of Biochemistry, University of Leicester, Leicester LE1 7RH, UK
    Hum Mol Genet 12:1337-48. 2003
    ....
  80. ncbi Transportin: nuclear transport receptor of a novel nuclear protein import pathway
    S Nakielny
    Howard Hughes Medical Institute, University of Pennsylvania, School of Medicine, Philadelphia, Pennsylvania, 19104 6148, USA
    Exp Cell Res 229:261-6. 1996
    ..Transportin relatives from Saccharomyces cerevisiae which likely serve as additional nuclear transport receptors are described...
  81. ncbi Mechanisms of fidelity in pre-mRNA splicing
    R Reed
    Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
    Curr Opin Cell Biol 12:340-5. 2000
    ....
  82. pmc HRP-2, the Caenorhabditis elegans homolog of mammalian heterogeneous nuclear ribonucleoproteins Q and R, is an alternative splicing factor that binds to UCUAUC splicing regulatory elements
    Jennifer L Kabat
    Department of Molecular, Cell and Developmental Biology and Center for Molecular Biology of RNA, University of California, Santa Cruz, California 95064, USA
    J Biol Chem 284:28490-7. 2009
    ..HRP-2, the Caenorhabditis elegans homolog of human heterogeneous nuclear ribonucleoproteins Q and R, binds to UCUAUC in the context of unc-52 intronic regulatory sequences as well as to ..
  83. ncbi Mutational definition of RNA-binding and protein-protein interaction domains of heterogeneous nuclear RNP C1
    L Wan
    Howard Hughes Medical Institute and Department of Biochemistry and Biophysics, University of Pennsylvania School of Medicine, Philadelphia 19104-6148, USA
    J Biol Chem 276:7681-8. 2001
    ..The experimental approach we described should be generally applicable for the screening and identification of amino acids that play a role in the binding of proteins to nucleic acid substrates...
  84. pmc The levels of the bancal product, a Drosophila homologue of vertebrate hnRNP K protein, affect cell proliferation and apoptosis in imaginal disc cells
    B Charroux
    Laboratoire de Genetique et Physiologie du Developpement, UMR 6545 CNRS Université, IBDM CNRS INSERM Université de la Méditerrannée, F 13288 Marseille Cedex 09, France
    Mol Cell Biol 19:7846-56. 1999
    ..Finally, the human K protein acts as a negative regulator of bancal gene expression. We propose that negative autoregulation limits the level of Bancal protein produced in vivo...
  85. ncbi Control of mRNA translation and stability in haematopoietic cells: the function of hnRNPs K and E1/E2
    Antje Ostareck-Lederer
    Department of Biochemistry and Biotechnology, Institute of Biochemistry, Martin Luther University, Kurt Mothes Str 3, 06120 Halle Saale, Germany
    Biol Cell 96:407-11. 2004
    ..regulation of gene expression in haematopoietic cells have uncovered that a subfamily of heterogeneous nuclear ribonucleoproteins (hnRNPs) is involved in cytoplasmic gene regulation...
  86. pmc Improved activities of CREB binding protein, heterogeneous nuclear ribonucleoproteins and proteasome following downregulation of noncoding hsromega transcripts help suppress poly(Q) pathogenesis in fly models
    Moushami Mallik
    Cytogenetics Laboratory, Department of Zoology, Banaras Hindu University, Varanasi, India
    Genetics 184:927-45. 2010
    ..reinforce the view that the noncoding hsromega RNA functions as a "hub" in cellular networks to maintain homeostasis by coordinating the functional availability of crucial cellular regulatory proteins...
  87. ncbi Haploinsufficiency of the germ cell-specific nuclear RNA binding protein hnRNP G-T prevents functional spermatogenesis in the mouse
    Ingrid Ehrmann
    Institute of Human Genetics, Newcastle University, Central Parkway, Newcastle NE1 3BZ, UK
    Hum Mol Genet 17:2803-18. 2008
    ..Our data show that haploinsufficiency of Hnrnpgt results in abnormal sperm production in the mouse. Genetic defects resulting in reduced levels of HNRNPGT could, therefore, be a cause of male infertility in humans...
  88. ncbi RBMY, a probable human spermatogenesis factor, and other hnRNP G proteins interact with Tra2beta and affect splicing
    J P Venables
    Department of Biochemistry, University of Leicester, University Road, Leicester LE1 7RH, UK
    Hum Mol Genet 9:685-94. 2000
    ..The RNA-binding domain of RBM affected 5' splice site selection. We conclude that the hnRNP G family of proteins is involved in pre-mRNA splicing and infer that RBM may be involved in Tra2beta-dependent splicing in spermatocytes...
  89. pmc Hrp59, an hnRNP M protein in Chironomus and Drosophila, binds to exonic splicing enhancers and is required for expression of a subset of mRNAs
    Eva Kiesler
    Department of Molecular Biology and Functional Genomics, Stockholm University, SE 10691 Stockholm, Sweden
    J Cell Biol 168:1013-25. 2005
    ..We also show that Hrp59 binds preferentially to exonic splicing enhancers and our results provide new insights into the role of hnRNP M in splicing regulation...
  90. ncbi A new regulatory protein, KSRP, mediates exon inclusion through an intronic splicing enhancer
    H Min
    Molecular Biology Institute, University of California at Los Angeles, 90095 1662, USA
    Genes Dev 11:1023-36. 1997
    ..Moreover, this inhibition of N1 splicing can be rescued by the addition of purified KSRP. KSRP is likely to regulate splicing from a number of intronic splicing enhancer sequences...
  91. ncbi Regulation of tissue-specific P-element pre-mRNA splicing requires the RNA-binding protein PSI
    C W Siebel
    Department of Molecular and Cell Biology, University of California, Berkeley 94720
    Genes Dev 8:1713-25. 1994
    ..The results indicate that PSI is an alternative splicing factor that regulates tissue-specific splicing, probably through interactions with generally expressed factors such as hrp48...
  92. pmc Heterogeneous nuclear ribonucleoprotein complexes and proteins in Drosophila melanogaster
    G Raychaudhuri
    Department of Microbiology, University of Virginia School of Medicine, Charlottesville 22908
    Mol Cell Biol 12:847-55. 1992
    ..melanogaster and mammals...
  93. ncbi The Drosophila hnRNPA/B homolog, Hrp48, is specifically required for a distinct step in osk mRNA localization
    Jean René Huynh
    The Wellcome Trust Cancer Research UK, Gurdon Institute and Department of Genetics, Cambridge University, Tennis Court Road, Cambridge CB2 1QR, United Kingdom
    Dev Cell 6:625-35. 2004
    ..This suggests a new step in the localization pathway, which may correspond to the assembly of Staufen/oskar mRNA transport particles...
  94. pmc hnRNP H binding at the 5' splice site correlates with the pathological effect of two intronic mutations in the NF-1 and TSHbeta genes
    Emanuele Buratti
    International Centre for Genetic Engineering and Biotechnology, 34012 Trieste, Italy
    Nucleic Acids Res 32:4224-36. 2004
    ..Thus, the reason why similar nucleotide substitutions can be either neutral or very disruptive of splicing function can be explained by the presence of specific binding signatures depending on local contexts...
  95. pmc Isolation of hnRNP complexes from Drosophila melanogaster
    M J Matunis
    Howard Hughes Medical Institute, University of Pennsylvania School of Medicine, Philadelphia 19104 6148
    J Cell Biol 116:245-55. 1992
    ..1992. J. Cell Biol. 116:257-269). The purification of D. melanogaster hnRNP complexes will facilitate genetic and cytological studies on the function of hnRNA-binding proteins and on the posttranscriptional regulation of gene expression...
  96. ncbi A second exon splicing silencer within human immunodeficiency virus type 1 tat exon 2 represses splicing of Tat mRNA and binds protein hnRNP H
    S Jacquenet
    , , Boulevard des Aiguillettes, BP239, , France
    J Biol Chem 276:40464-75. 2001
    ..We propose that hnRNP H binds to the HIV-1 ESS2p element and competes with U2AF(35) for binding to the exon sequence flanking 3'-splice site A3. This binding results in the inhibition of splicing at 3'-splice site A3...
  97. pmc Altered levels of the Drosophila HRB87F/hrp36 hnRNP protein have limited effects on alternative splicing in vivo
    K Zu
    Department of Microbiology, University of Virginia, Charlottesville 22908, USA
    Mol Biol Cell 7:1059-73. 1996
    ....
  98. pmc The human hnRNP-M proteins: structure and relation with early heat shock-induced splicing arrest and chromosome mapping
    R Gattoni
    Institut de Genetique et de Biologie Moleculaire et Cellulaire, CNRS INSERM ULP, C U de Strasbourg, France
    Nucleic Acids Res 24:2535-42. 1996
    ..Taken together, our data suggest that these proteins are involved in splicing as well as in early stress-induced splicing arrest. Further in situ hybridization assays located the hnRNP-M encoding gene on human chromosome 19...
  99. pmc Association of individual hnRNP proteins and snRNPs with nascent transcripts
    E L Matunis
    Howard Hughes Medical Institute, University of Pennsylvania School of Medicine, Philadelphia 19104 6148
    J Cell Biol 121:219-28. 1993
    ..The distinct and specific constellation of hnRNP proteins and snRNP particles that assembles on different pre-mRNAs is likely to affect the fate and pathway of processing of these transcripts...
  100. ncbi HRP-2, a heterogeneous nuclear ribonucleoprotein, is essential for embryogenesis and oogenesis in Caenorhabditis elegans
    Jane H Kinnaird
    Veterinary Parasitology, Institute of Comparative Medicine, University of Glasgow, Glasgow G61 1QH, UK
    Exp Cell Res 298:418-30. 2004
    b>Heterogeneous nuclear ribonucleoproteins (hnRNPs) have fundamental roles in the posttranscriptional control of gene expression...
  101. ncbi Vaccinia virus late transcription is activated in vitro by cellular heterogeneous nuclear ribonucleoproteins
    C F Wright
    Department of Pathology and Laboratory Medicine, Medical University of South Carolina, Charleston, South Carolina 29425, USA
    J Biol Chem 276:40680-6. 2001
    ..Here, extensive purification of VLTF-X has revealed that heterogeneous nuclear ribonucleoproteins A2/B1 and RBM3 co-purified with in vitro late transcription stimulation...

Research Grants63

  1. Vitamin D and Estrogen Action in Bone: Concerted Role for hnRNPs in the C Family
    John S Adams; Fiscal Year: 2013
    ....
  2. MOLECULAR MIMICRY IN IMMUNE MEDIATED NEUROLOGIC DISEASE
    Michael Levin; Fiscal Year: 2013
    ..The combination of both a diagnostic and pathogenic immune reaction is of particular relevance and has the potential to make important contributions to the long- term care and outcomes of people with MS. ..
  3. MOLECULAR MODELS OF IMMUNE-MEDIATED VASCULAR INJURY
    Jeffrey R Bender; Fiscal Year: 2012
    ....
  4. Alternative Splicing of the Insulin Receptor Gene
    NICHOLAS J WEBSTER; Fiscal Year: 2013
    ..We will determine whether these mice are insulin-resistant using a panel of metabolic tests and we will assess other potential targets for SRp20 and SF2 in the liver using genomic approaches. ..
  5. Novel mouse models of inflammation-resolution
    Paul L Fox; Fiscal Year: 2010
    ..These models may reveal alternative targets for novel anti-inflammatory therapeutic strategies that, because of their specificity, may exhibit minimal adverse side-effects. ..
  6. Repair of oxidative damage in mammalian genomes
    Tapas K Hazra; Fiscal Year: 2010
    ..abstract_text> ..
  7. Regulation of caspase 9 RNA splicing in NSCLC cancer
    Charles E Chalfant; Fiscal Year: 2013
    ....
  8. The Role of Caspase 9b Expression in NSCLC Survival/Chemotherapeutic Sensitivity
    Charles E Chalfant; Fiscal Year: 2013
    ..g. cisplatinum and paclitaxel). ..
  9. PLASTICITY IN THE AGING OLFACTORY SYSTEM
    John W Cave; Fiscal Year: 2013
    ..This proposal will significantly advance the mechanistic understanding of both OB DA neuronal differentiation and the generation of neuronal phenotypic diversity. ..
  10. hnRNP A1 inhibition of HIV-1 replication
    Massimo Caputi; Fiscal Year: 2011
    ..We propose to create to deliver this gene, or portions of it, in leukocytes, the primary target of the virus, to inhibit viral replication and block the progression of the infection. ..
  11. Molecular mechanism of omega-3 response
    JAMES T BRENNA; Fiscal Year: 2013
    ..Human genetic variation and metabolic conditions responsive to omega-3 LCPUFA supplementation will be identified. ..
  12. Mechanism of Folate Deficiency as a Co-Factor for HPV16-induced Carcinogenesis
    ASOK ANTONY; Fiscal Year: 2013
    ..nutrition;Veterans with HPV16 and human immunodeficiency virus (HIV);and those in developing countries where the combination of poor nutrition, and co-infection with HIV and HPV16 places individuals at high risk for HPV-induced cancers ..
  13. Crohn's disease-associated NOD2 Mutants
    Xiaojing Ma; Fiscal Year: 2010
    ..We propose to investigate the mechanisms whereby R702W and G908R regulate IL-12/IL-23 gene expression. ..
  14. Role of microRNAs in the regulation of CML stem cell survival and self renewal
    Danilo Perrotti; Fiscal Year: 2013
    ..Hence, the strong importance and high relevance of this work for basic and translational cancer research. ..
  15. TGF-regulated EMT
    Philip H Howe; Fiscal Year: 2013
    ....
  16. A protein-directed RNA switch that regulates translation
    Paul L Fox; Fiscal Year: 2013
    ..We also speculate that the VEGF-A switch may represent the founding member of a family of protein-directed RNA switches in vertebrates that integrate physiological or pathological stimuli to control gene expression. ..
  17. Role of RNA Binding Proteins in BCR/ABL Leukemogenesis
    Danilo Perrotti; Fiscal Year: 2012
    ..Thus, it is clear that, if successful, the proposed research will have a strong impact on leukemia research and patient care. ..
  18. RNA foci in C9FTD/ALS
    Leonard Petrucelli; Fiscal Year: 2013
    ..Overall, we believe the proposed studies examining RNA foci formation consequences will lead to a better understanding of C9FTD/ALS-related mechanisms and help to uncover promising therapeutic targets. ..
  19. POLIOVIRUS GENE FUNCTION AND REGULATION
    Bert L Semler; Fiscal Year: 2013
    ..It will also analyze the role of host proteins in picornavirus RNA synthesis. The proposed research will enhance our knowledge of virus-host interactions and identify potential new targets for anti-viral therapeutics. ..
  20. Alternative pre-mRNA Splicing in Drosophila
    Donald C Rio; Fiscal Year: 2013
    ....
  21. Molecular Basis of rCGG-Mediated Neurodegeneration
    DAVID LOREN NELSON; Fiscal Year: 2013
    ..Here we will use both Drosophila and mouse models to further test the hypothesis that FXTAS results from abnormal RNA metabolism that stems from inappropriate association of RBPs with the RNA produced by FMR1 premutation alleles. ..
  22. Examining the role of Cbk1/NDR kinase in regulating mRNA localization
    Francis C Luca; Fiscal Year: 2013
    ..As such, these studies may reveal a key mechanism for NDR kinases in cell polarity, neuronal morphogenesis and tumor suppressor activity. ..
  23. BIOCHEMISTRY OF REGULATED PRE-MRNA SPLICING/DROSOPHILA
    Donald C Rio; Fiscal Year: 2010
    ..4. Analyze the structure and function of PSI, Drosophila U2AF and U2AF-related proteins. ..
  24. RNA Binding Proteins in Cancer
    Shrikant Anant; Fiscal Year: 2013
    ..abstract_text> ..
  25. Regulation of Selenoprotein Synthesis by SECIS-binding Proteins
    Donna M Driscoll; Fiscal Year: 2013
    ..By identifying these regulatory pathways, our studies will provide a strong foundation for developing more specific and targeted approaches for modulating selenoprotein expression in vivo. ..
  26. METABOLIC CONTROL OF PROINSULIN BIOSYNTHESIS TRANSLATION
    Christopher J Rhodes; Fiscal Year: 2013
    ..Indeed, in the spirit of translational medicine, the feasibility of such a strategy will also be examined in preclinical models in this proposal. ..
  27. Pathways of T cell Senescence
    ABBE DE VALLEJO; Fiscal Year: 2009
    ....
  28. Translational Control by eIF3f in Pancreatic Cancer
    Eugene W Gerner; Fiscal Year: 2012
    ..Thus, a better understanding of translational regulation is essential in deciphering the molecular basis underlying pancreatic cancer development, as well as providing a foundation for developing new anti-cancer strategies. ..
  29. Computational and experimental modeling of RNA Splicing
    Michael Q Zhang; Fiscal Year: 2012
    ....
  30. RNA-Gain-of-Function Pathogenesis in SCA10
    Tetsuo Ashizawa; Fiscal Year: 2013
    ..These studies will firmly establish the pathogenic role of hnRNP K and provide a therapeutic target. ..
  31. Rational targeting of protein translation of cancer treatments
    DAVID JOSEPH WEBER; Fiscal Year: 2013
    ..This could be accomplished by solving hnRNP A18 three dimensional structure and use computer-aided drug design (CADD) technology to identify small molecules that could target hnRNP A18. ..
  32. Mechanisms of Signal-Induced Alternative Splicing: CD45
    Kristen W Lynch; Fiscal Year: 2013
    ....
  33. Regulation of Proinflammatory Signaling Pathways in CFTR
    HARVEY BRUCE POLLARD; Fiscal Year: 2010
    ..The proposal is both unique and innovative by directing the investigation to the biology and chemistry of the IL-8 promoter in CF lung epithelial cells, both in vitro and in vivo. ..
  34. Neurobiology of Social Behavior
    Kim L Huhman; Fiscal Year: 2013
    ..Advancing our understanding of the mechanisms involved in shifts among stable behavioral states will dramatically advance our ability to treat intractable mood and anxiety disorders such as depression and PTSD. ..
  35. Deconvoluting G-quadruplex and i-Motif dynamics: the role of the physiological st
    Randy M Wadkins; Fiscal Year: 2013
    ..Additionally, in support of the AREA program goals, this work provides hands-on learning for students at the University of Mississippi, who will study fundamental questions regarding higher-order DNA dynamics. ..
  36. Ebola virus VP24 alters hnRNP C nuclear import: implications for replication
    REED SOLOMON SHABMAN; Fiscal Year: 2010
    ....
  37. Integrative Analysis of Wilson's Disease
    Svetlana Lutsenko; Fiscal Year: 2013
    ..Cell injury and behavior changes will be evaluated in conjunctions with analysis of molecular and metabolic changes. ..
  38. NIH Support of Conferences and Scientific Meetings
    Katheleen Gardiner; Fiscal Year: 2013
    ..It is anticipated that this conference will lead to new hypotheses regarding pharmacological treatment of cognitive deficits in DS, foster new collaborations and bring new researchers with novel expertise into the field of DS. ..
  39. Structural Biophysics of HIV Alternative Splicing
    BLANTON SMITH TOLBERT; Fiscal Year: 2013
    ....
  40. Functional Analysis of an hnRNP C-Like Protein, p542
    JAMES MCAFEE; Fiscal Year: 2004
    ..conserves important primary structural and functional motifs that have been identified in the heterogeneous nuclear ribonucleoproteins C1 and C2 (hnRNP C), two very abundant nuclear proteins associated with pre-mRNA processing, ..
  41. Regulation of HIV-1 RNA Processing
    Massimo Caputi; Fiscal Year: 2007
    ..Possible coupling of HIV-1 transcription and splicing will be investigated, using both a coupled transcription-splicing assay and transient transfection experiments. ..
  42. Chemoprevention of Early Pulmonary Lesions in Mice
    ALVIN MALKINSON; Fiscal Year: 2004
    ..Our goals are: (1) to successfully inhibit lung tumor progression when anti- inflammatory drugs are applied after early lesions have forms; and (2) to identify novel early-stage predictive biomarkers. ..
  43. Mechanisms Regulating Alternative pre-mRNA Splicing
    James Patton; Fiscal Year: 2005
    ..Overall, the experiments in this proposal seek to understand the mechanistic basis underlying the repression and activation of splicing by SR proteins and hnRNP proteins. ..
  44. REGULATORY MECHANISMS IN PROTEIN BIOSYNTHESIS
    WLODZIMIERZ SZER; Fiscal Year: 1990
    ....
  45. Novel IRES-specific trans acting factors that modulate EV71 translation
    Mei Ling Li; Fiscal Year: 2007
    ..Specifically we shall use siRNA to knock down levels of endogenous FBP and hnRNP A1 in EV71-infected cells and investigate the effect of depletion of these proteins on the translation of EV71. [unreadable] [unreadable] [unreadable]..
  46. LYMPHOKINE MRNA BINDING PROTEINS
    William Rigby; Fiscal Year: 1999
    ..Thus, understanding the role of these trans-acting factors in lymphokine gene expression will have direct relevance to immunobiology and growth regulation, as well as the clinical disorders manifest by their dysregulation. ..
  47. Regulation of cell cycle genes by K protein
    Karol Bomsztyk; Fiscal Year: 2003
    ..The proposed studies will explain the need of proliferating and tumor cells to alter K protein expression. ..
  48. Adrenergic Receptor Mechanisms in Antidepressant Therapy
    CURTIS MACHIDA; Fiscal Year: 2004
    ..The primary experimental objectives of Specific Aim 2 are to validate ICER and the novel bZlP-like transcription factor as potential repressors of beta1-AR gene expression during antidepressant therapy. ..
  49. MECHANISM OF CORONAVIRUS RNA TRANSCRIPTION
    Xuming Zhang; Fiscal Year: 1999
    ..These studies will contribute to the fundamental understanding of the mechanisms of regulation of RNA synthesis in coronaviruses and other RNA viruses as well. ..
  50. HCV CORE PROTEIN AND HEPATOCYTE GROWTH REGULATION
    Ranjit Ray; Fiscal Year: 2001
    ..The long term goal of this study is to understand the molecular mechanism of HCV core protein mediated pathogenesis and to design effective strategies for intervention of HCV related disease in humans. ..
  51. RIBONUCLEOPROTEIN COMPLEXES IN RENAL CARCINOMA
    David Irwin; Fiscal Year: 1980
    ....
  52. NUDR--A Potential Tumor Suppressor of Childhood Cancers
    Jodi Huggenvik; Fiscal Year: 2005
    ..abstract_text> ..
  53. Polyadenylation Factors in B-Cells, Lymphoma & Myeloma
    Christine Milcarek; Fiscal Year: 2007
    ..Understanding the control of polyadenylation is important for understanding and possibly controlling aspects of cell growth, differentiation and malignancy. ..
  54. Mechanisms of AKT-dependent sensitivity to rapamycin
    Joseph F Gera; Fiscal Year: 2012
    ..The mechanisms of cell intrinsic resistance to these compounds will be investigated, as well as, the pre-clinical evaluation of combination targeted therapies in glioma in vitro and in vivo models. ..
  55. Regulation of c-myc translation by hnRNP A1: role in multiple myeloma tumor respo
    Alan K Lichtenstein; Fiscal Year: 2013
    ..abstract_text> ..
  56. Mechanisms of Bronchial Myogenesis
    Lucia Schuger; Fiscal Year: 2005
    ..abstract_text> ..
  57. Pathogenic Mechanism of Spinocerebellar Ataxia Type 10
    Tetsuo Ashizawa; Fiscal Year: 2009
    ..Establishing the mechanism in SCA10 will fill the current gap of knowledge and facilitate the development of rational therapy. ..
  58. REGULATION OF COMPLEMENT RECEPTOR GENE EXPRESSION
    George Tsokos; Fiscal Year: 2002
    ..Since we have found that sera from patients with systemic autoimmune diseases have antibodies directed against this hnRNP, we propose to study their frequency and clinical significance. ..
  59. RIBONUCLEOPROTEINS, MRNA AND CYTOSKELETAL STRUCTURES
    Gideon Dreyfuss; Fiscal Year: 1991
    ..From these studies a better picture of ho mRNA is formed, maintained and functions in animal cells is likely to emerge...
  60. MURINE CORONAVIRUS RNA SYNTHESIS
    Michael Lai; Fiscal Year: 2004
    ..Finally, the potential effects of viral RNA synthesis on the cellular RNA processing, with particular emphasis on the possible alterations of alternative RNA splicing will be examined. ..
  61. THE YEAST NUCLEAR PORE COMPLEX
    Michael Douglas; Fiscal Year: 1991
    ....
  62. MINORITY PREDOCTORAL FELLOWSHIP PROGRAM
    DENISE HAMPTON; Fiscal Year: 2003
    Nuclear pre-messenger RNA transcripts in mammalian cells are complexed with proteins known as heterogeneous nuclear ribonucleoproteins (hnRNPs)...
  63. Assembly and export of HIV messenger ribonucleoproteins
    JOHN ROBERT HOGG; Fiscal Year: 2010
    ..This research is intended to identify new human proteins necessary for viral gene expression, and, by extension, viral infectivity. Proteins involved in these processes represent attractive targets for therapeutic intervention. ..