ribonucleoproteins

Summary

Summary: Complexes of RNA-binding proteins with ribonucleic acids (RNA).

Top Publications

  1. doi The spliceosome: design principles of a dynamic RNP machine
    Markus C Wahl
    Makromolekulare Röntgenkristallographie, Max Planck Institut fur biophysikalische Chemie, Am Fassberg 11, D 37077 Gottingen, Germany
    Cell 136:701-18. 2009
  2. pmc Eukaryotic stress granules: the ins and outs of translation
    J Ross Buchan
    Howard Hughes Medical Institute, University of Arizona, Tucson, 85721, USA
    Mol Cell 36:932-41. 2009
  3. ncbi Messenger-RNA-binding proteins and the messages they carry
    Gideon Dreyfuss
    Howard Hughes Medical Institute and Department of Biochemistry and Biophysics, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104 6148, USA
    Nat Rev Mol Cell Biol 3:195-205. 2002
  4. doi RNA granules: post-transcriptional and epigenetic modulators of gene expression
    Paul Anderson
    Division of Rheumatology, Immunology and Allergy, Brigham and Women s Hospital and Harvard Medical School, Boston, Massachusetts 02115, USA
    Nat Rev Mol Cell Biol 10:430-6. 2009
  5. doi Frequent pathway mutations of splicing machinery in myelodysplasia
    Kenichi Yoshida
    Cancer Genomics Project, Graduate School of Medicine, The University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 8655, Japan
    Nature 478:64-9. 2011
  6. doi The structure of native influenza virion ribonucleoproteins
    Rocio Arranz
    Department of Macromolecular Structure, Centro Nacional de Biotecnología Consejo Superior de Investigaciones Cienfícas CSIC, Madrid, Spain
    Science 338:1634-7. 2012
  7. pmc Polysomes, P bodies and stress granules: states and fates of eukaryotic mRNAs
    Vidya Balagopal
    Department of Molecular and Cellular Biology and Howard Hughes Medical Institute, University of Arizona, Tucson, AZ 85721 0206, USA
    Curr Opin Cell Biol 21:403-8. 2009
  8. ncbi Switching from repression to activation: microRNAs can up-regulate translation
    Shobha Vasudevan
    Department of Molecular Biophysics and Biochemistry, Howard Hughes Medical Institute, Yale University School of Medicine, Boyer Center for Molecular Medicine, 295 Congress Avenue, New Haven, CT 06536, USA
    Science 318:1931-4. 2007
  9. pmc P bodies promote stress granule assembly in Saccharomyces cerevisiae
    J Ross Buchan
    Department of Molecular and Cellular Biology, Howard Hughes Medical Institute, University of Arizona, Tucson, AZ 85721, USA
    J Cell Biol 183:441-55. 2008
  10. ncbi Regulation of Fas alternative splicing by antagonistic effects of TIA-1 and PTB on exon definition
    Jose Maria Izquierdo
    Centre de Regulacio Genomica, Passeig Maritim 37 49, 08003 Barcelona, Spain
    Mol Cell 19:475-84. 2005

Detail Information

Publications421 found, 100 shown here

  1. doi The spliceosome: design principles of a dynamic RNP machine
    Markus C Wahl
    Makromolekulare Röntgenkristallographie, Max Planck Institut fur biophysikalische Chemie, Am Fassberg 11, D 37077 Gottingen, Germany
    Cell 136:701-18. 2009
    b>Ribonucleoproteins (RNPs) mediate key cellular functions such as gene expression and its regulation...
  2. pmc Eukaryotic stress granules: the ins and outs of translation
    J Ross Buchan
    Howard Hughes Medical Institute, University of Arizona, Tucson, 85721, USA
    Mol Cell 36:932-41. 2009
    ..Together, stress granules and P-bodies reveal a dynamic cycle of distinct biochemical and compartmentalized mRNPs in the cytosol, with implications for the control of mRNA function...
  3. ncbi Messenger-RNA-binding proteins and the messages they carry
    Gideon Dreyfuss
    Howard Hughes Medical Institute and Department of Biochemistry and Biophysics, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104 6148, USA
    Nat Rev Mol Cell Biol 3:195-205. 2002
    ..These proteins communicate crucial information to the translation machinery for the surveillance of nonsense mutations and for mRNA localization and translation...
  4. doi RNA granules: post-transcriptional and epigenetic modulators of gene expression
    Paul Anderson
    Division of Rheumatology, Immunology and Allergy, Brigham and Women s Hospital and Harvard Medical School, Boston, Massachusetts 02115, USA
    Nat Rev Mol Cell Biol 10:430-6. 2009
    The composition of cytoplasmic messenger ribonucleoproteins (mRNPs) is determined by their nuclear and cytoplasmic histories and reflects past functions and future fates...
  5. doi Frequent pathway mutations of splicing machinery in myelodysplasia
    Kenichi Yoshida
    Cancer Genomics Project, Graduate School of Medicine, The University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 8655, Japan
    Nature 478:64-9. 2011
    ..Our results provide the first evidence indicating that genetic alterations of the major splicing components could be involved in human pathogenesis, also implicating a novel therapeutic possibility for myelodysplasia...
  6. doi The structure of native influenza virion ribonucleoproteins
    Rocio Arranz
    Department of Macromolecular Structure, Centro Nacional de Biotecnología Consejo Superior de Investigaciones Cienfícas CSIC, Madrid, Spain
    Science 338:1634-7. 2012
    ..segmented negative-stranded RNAs are associated with the polymerase complex and nucleoprotein (NP), forming ribonucleoproteins (RNPs), which are responsible for virus transcription and replication...
  7. pmc Polysomes, P bodies and stress granules: states and fates of eukaryotic mRNAs
    Vidya Balagopal
    Department of Molecular and Cellular Biology and Howard Hughes Medical Institute, University of Arizona, Tucson, AZ 85721 0206, USA
    Curr Opin Cell Biol 21:403-8. 2009
    ..Here we discuss how these different granules suggest a dynamic model for the metabolism of cytoplasmic mRNAs wherein they cycle between different mRNP states with different functional properties and subcellular locations...
  8. ncbi Switching from repression to activation: microRNAs can up-regulate translation
    Shobha Vasudevan
    Department of Molecular Biophysics and Biochemistry, Howard Hughes Medical Institute, Yale University School of Medicine, Boyer Center for Molecular Medicine, 295 Congress Avenue, New Haven, CT 06536, USA
    Science 318:1931-4. 2007
    ..Argonaute (AGO) and fragile X mental retardation-related protein 1 (FXR1), factors associated with micro-ribonucleoproteins (microRNPs)...
  9. pmc P bodies promote stress granule assembly in Saccharomyces cerevisiae
    J Ross Buchan
    Department of Molecular and Cellular Biology, Howard Hughes Medical Institute, University of Arizona, Tucson, AZ 85721, USA
    J Cell Biol 183:441-55. 2008
    ..These observations argue that P bodies are important sites for decisions of mRNA fate and that stress granules, at least in yeast, primarily represent pools of mRNAs stalled in the process of reentry into translation from P bodies...
  10. ncbi Regulation of Fas alternative splicing by antagonistic effects of TIA-1 and PTB on exon definition
    Jose Maria Izquierdo
    Centre de Regulacio Genomica, Passeig Maritim 37 49, 08003 Barcelona, Spain
    Mol Cell 19:475-84. 2005
    ..We propose that TIA-1 and PTB regulate Fas splicing and possibly Fas-mediated apoptosis by targeting molecular events that lead to exon definition...
  11. pmc Calreticulin: one protein, one gene, many functions
    M Michalak
    MRC Group in Molecular Biology of Membranes, Department of Biochemistry, University of Alberta, 3 56 Medical Sciences Building, Edmonton, Alberta, Canada T6G 2H7
    Biochem J 344:281-92. 1999
    ..Studies on the cell biology of calreticulin revealed that the ER membrane is a very dynamic intracellular compartment affecting many aspects of cell physiology...
  12. doi hnRNP A1 proofreads 3' splice site recognition by U2AF
    João Paulo Tavanez
    Centre de Regulacio Genomica, Dr Aiguader 88, 08003 Barcelona, Spain Universitat Pompeu Fabra, Dr Aiguader 88, 08003 Barcelona, Spain
    Mol Cell 45:314-29. 2012
    ..Consistent with the functional relevance of this activity for splicing, proofreading assays reveal a role for hnRNP A1 in U2AF-mediated recruitment of U2 snRNP to the pre-mRNA...
  13. pmc Subcellular mRNA localization in animal cells and why it matters
    Christine E Holt
    Department of Physiology, Development, and Neuroscience, University of Cambridge, Cambridge CB2 3DY, UK
    Science 326:1212-6. 2009
    ..Here, we review current knowledge of the mechanisms and functions of mRNA localization in animal cells...
  14. pmc Stress-specific composition, assembly and kinetics of stress granules in Saccharomyces cerevisiae
    J Ross Buchan
    Howard Hughes Medical Institute, University of Arizona, Tucson, AZ 85721, USA
    J Cell Sci 124:228-39. 2011
    ..These results indicate that stress granule assembly, kinetics and composition in yeast can vary in a stress-specific manner, which we suggest reflects different rate-limiting steps in a common mRNP remodeling pathway...
  15. pmc Antibodies mediate intracellular immunity through tripartite motif-containing 21 (TRIM21)
    Donna L Mallery
    Protein and Nucleic Acid Chemistry Division, Medical Research Council Laboratory of Molecular Biology, Cambridge CB2 0QH, United Kingdom
    Proc Natl Acad Sci U S A 107:19985-90. 2010
    ..These results reveal an intracellular arm of adaptive immunity in which the protection mediated by antibodies does not end at the cell membrane but continues inside the cell to provide a last line of defense against infection...
  16. pmc P-body formation is a consequence, not the cause, of RNA-mediated gene silencing
    Ana Eulalio
    Max Planck Institute for Developmental Biology, Spemannstrasse 35, D 72076 Tubingen, Germany
    Mol Cell Biol 27:3970-81. 2007
    ..Thus, even though P-body components play crucial roles in mRNA silencing and decay, aggregation into P bodies is not required for function but is instead a consequence of their activity...
  17. doi Stress granules: the Tao of RNA triage
    Paul Anderson
    Division of Rheumatology, Immunology and Allergy, Brigham and Women s Hospital and Harvard Medical School, One Jimmy Fund Way, Boston, MA 02115, USA
    Trends Biochem Sci 33:141-50. 2008
    ..Thus, these ephemeral bodies represent more than just the dynamic sorting of mRNA between translation and decay...
  18. ncbi Development of autoantibodies before the clinical onset of systemic lupus erythematosus
    Melissa R Arbuckle
    Arthritis and Immunology Program, Oklahoma Medical Research Foundation, Oklahoma City, OK 73104, USA
    N Engl J Med 349:1526-33. 2003
    ..We investigated the onset and progression of autoantibody development before the clinical diagnosis...
  19. ncbi Nrd1 interacts with the nuclear exosome for 3' processing of RNA polymerase II transcripts
    Lidia Vasiljeva
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 240 Longwood Avenue, Boston, Massachusetts 02115, USA
    Mol Cell 21:239-48. 2006
    ..Since Nrd1 is known to bind RNA polymerase II and be important for sn/snoRNA 3' end processing, Nrd1 may link transcription and RNA 3' end formation with surveillance by the exosome...
  20. doi Keeping mRNPs in check during assembly and nuclear export
    Evelina Tutucci
    Department of Cell Biology and Frontiers in Genetics, National Center for Competence in Research NCCR, Sciences III, Geneva, Switzerland
    Nat Rev Mol Cell Biol 12:377-84. 2011
    ..genetic information and the machineries that control the production of mature and export-competent messenger ribonucleoproteins (mRNPs), a multistep process that is regulated in a spatial and temporal manner...
  21. pmc Organization of the influenza virus replication machinery
    Arne Moeller
    National Resource for Automated Molecular Microscopy, Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Science 338:1631-4. 2012
    ..Observations of branched-RNP structures in negative-stain electron microscopy and their putative identification as replication intermediates suggest a mechanism for viral replication by a second polymerase on the RNP template...
  22. pmc Stress-dependent relocalization of translationally primed mRNPs to cytoplasmic granules that are kinetically and spatially distinct from P-bodies
    Nathaniel P Hoyle
    Faculty of Life Sciences, University of Manchester, Manchester M13 9PT, England, UK
    J Cell Biol 179:65-74. 2007
    ..Overall, this study highlights new potential control points in both the regulation of mRNA fate and the global control of translation initiation...
  23. pmc Genome-wide function of THO/TREX in active genes prevents R-loop-dependent replication obstacles
    Belén Gómez-González
    Centro Andaluz de Biologia Molecular y Medicina Regenerativa CABIMER, Universidad de Sevilla CSIC, Sevilla, Spain
    EMBO J 30:3106-19. 2011
    ..Our work establishes a genome-wide function for THO-Sub2 in transcription elongation and mRNP biogenesis that function to prevent the accumulation of transcription-mediated replication obstacles, including R-loops...
  24. pmc The protein factors MBNL1 and U2AF65 bind alternative RNA structures to regulate splicing
    M Bryan Warf
    Institute of Molecular Biology and Department of Chemistry, University of Oregon, Eugene, OR 97403, USA
    Proc Natl Acad Sci U S A 106:9203-8. 2009
    ..Thus, U2AF65 binding can be blocked either by MBNL1 binding or by the stabilization of RNA secondary structure...
  25. pmc The exon-exon junction complex provides a binding platform for factors involved in mRNA export and nonsense-mediated mRNA decay
    H Le Hir
    Howard Hughes Medical Institute, Department of Biochemistry, Brandeis University, 415 South Street, Waltham, MA 02454, USA
    EMBO J 20:4987-97. 2001
    ..Finally, we show that the composition of the EJC is dynamic in vivo and is subject to significant evolution upon mRNA export to the cytoplasm...
  26. pmc Proteomic and functional analysis of Argonaute-containing mRNA-protein complexes in human cells
    Julia Höck
    Laboratory of RNA Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany
    EMBO Rep 8:1052-60. 2007
    ..Separation of Ago-associated messenger ribonucleoproteins (mRNPs) showed that Ago1 and Ago2 reside in three complexes with distinct Dicer and RNA-induced silencing ..
  27. pmc Global analysis of yeast mRNPs
    Sarah F Mitchell
    Department of Chemistry and Biochemistry, University of Colorado Boulder, Boulder, Colorado, USA
    Nat Struct Mol Biol 20:127-33. 2013
    ..When taken together, this work defines the major yeast mRNP proteins, reveals widespread changes in their subcellular location during stress and begins to define assembly rules for P-body mRNPs...
  28. pmc Architecture and nucleic acids recognition mechanism of the THO complex, an mRNP assembly factor
    Alvaro Peña
    Department of Structure of Macromolecules, Centro Nacional de Biotecnologia CNB CSIC, Madrid, Spain
    EMBO J 31:1605-16. 2012
    ..In summary, this study describes the THO architecture, the structural basis for its chromatin targeting, and highlights the importance of unfolded regions of eukaryotic proteins...
  29. pmc Influenza A virus utilizes suboptimal splicing to coordinate the timing of infection
    Mark A Chua
    Microbiology Graduate School Training Program, Mount Sinai School of Medicine, New York, NY 10029, USA
    Cell Rep 3:23-9. 2013
    ..Together, these data demonstrate that coordination of the influenza A virus life cycle is set by a "molecular timer" that operates on the inefficient splicing of a virus transcript...
  30. ncbi RIP-Chip: the isolation and identification of mRNAs, microRNAs and protein components of ribonucleoprotein complexes from cell extracts
    Jack D Keene
    Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, North Carolina 27710, USA
    Nat Protoc 1:302-7. 2006
    ..Using RIP-Chip, the identification and/or quantification of RNAs in RNP complexes can be accomplished within a few hours or days depending on the RNA detection method used...
  31. pmc Loss of the lupus autoantigen Ro52/Trim21 induces tissue inflammation and systemic autoimmunity by disregulating the IL-23-Th17 pathway
    Alexander Espinosa
    Department of Medicine, Karolinska Institute, Stockholm SE 171 77, Sweden
    J Exp Med 206:1661-71. 2009
    ....
  32. doi Biogenesis of mRNPs: integrating different processes in the eukaryotic nucleus
    Rosa Luna
    Centro Andaluz de Biologia Molecular y Medicina Regenerativa CABIMER, Universidad de Sevilla CSIC, Seville, Spain
    Chromosoma 117:319-31. 2008
    ..Here, we review the overall process of mRNP biogenesis with particular emphasis on the functional coupling of transcription with mRNP biogenesis and export and its relationship to nuclear organization...
  33. pmc The spliceosome deposits multiple proteins 20-24 nucleotides upstream of mRNA exon-exon junctions
    H Le Hir
    Howard Hughes Medical Institute, Department of Biochemistry, Brandeis University, 415 South Street, Waltham, MA 02454, USA
    EMBO J 19:6860-9. 2000
    ..Possible functions for this complex in nucleocytoplasmic transport of spliced mRNA, as well as the nonsense-mediated mRNA decay pathway, are discussed...
  34. doi Protein-RNA interactions: new genomic technologies and perspectives
    Julian König
    Medical Research Council Laboratory of Molecular Biology, Hills Road, Cambridge CB2 0QH, UK
    Nat Rev Genet 13:77-83. 2011
    ..Finally, we highlight current challenges in data analysis and the future steps that are needed to obtain a quantitative and high-resolution picture of protein-RNA interactions on a genome-wide scale...
  35. ncbi Inhibition of translational initiation by Let-7 MicroRNA in human cells
    Ramesh S Pillai
    Friedrich Miescher Institute for Biomedical Research, 4002 Basel, Switzerland
    Science 309:1573-6. 2005
    ..Repressed mRNAs, Ago proteins, and miRNAs were all found to accumulate in processing bodies. We propose that localization of mRNAs to these structures is a consequence of translational repression...
  36. pmc Biogenesis of telomerase ribonucleoproteins
    Emily D Egan
    Department of Molecular and Cell Biology, University of California at Berkeley, Berkeley, California 94720 3200, USA
    RNA 18:1747-59. 2012
    ..This review highlights the diversity of strategies for telomerase RNP biogenesis, RNP assembly, and telomere recruitment among ciliates, yeasts, and vertebrates and suggests common themes in these pathways and their regulation...
  37. doi Dynamics of single mRNP nucleocytoplasmic transport and export through the nuclear pore in living cells
    Amir Mor
    The Mina and Everard Goodman Faculty of Life Sciences and Institute of Nanotechnology, Bar Ilan University, Ramat Gan 52900, Israel
    Nat Cell Biol 12:543-52. 2010
    ....
  38. ncbi Mutations in the RNA component of RNase MRP cause a pleiotropic human disease, cartilage-hair hypoplasia
    M Ridanpaa
    Folkhalsan Institute of Genetics, 00280 Helsinki, Finland
    Cell 104:195-203. 2001
    ..The association of protein subunits with RNA appears unaltered. We conclude that mutations in RMRP cause CHH by disrupting a function of RNase MRP RNA that affects multiple organ systems...
  39. pmc The structure of a biologically active influenza virus ribonucleoprotein complex
    Rocío Coloma
    Centro Nacional de Biotecnologia, Campus de Cantoblanco, Madrid, Spain
    PLoS Pathog 5:e1000491. 2009
    ..The structure reported here will serve as a framework to generate a quasi-atomic model of the molecular machine responsible for viral RNA synthesis and to test new models for virus RNA replication and transcription...
  40. ncbi hnRNP K: an HDM2 target and transcriptional coactivator of p53 in response to DNA damage
    Abdeladim Moumen
    The Wellcome Trust and Cancer Research UK Gurdon Institute and Department of Zoology, Cambridge University, Tennis Court Road, Cambridge CB2 1QN, United Kingdom
    Cell 123:1065-78. 2005
    ..These findings establish hnRNP K as a new HDM2 target and show that, by serving as a cofactor for p53, hnRNP K plays key roles in coordinating transcriptional responses to DNA damage...
  41. doi Multi-domain conformational selection underlies pre-mRNA splicing regulation by U2AF
    Cameron D Mackereth
    Institute of Structural Biology, Helmholtz Zentrum Munchen, Ingolstadter Landstrasse 1, 85764 Neuherberg, Germany
    Nature 475:408-11. 2011
    ..Similar mechanisms of cooperative multi-domain conformational selection may operate more generally in the recognition of degenerate nucleotide or amino acid motifs by multi-domain proteins...
  42. doi THO/Sub2p functions to coordinate 3'-end processing with gene-nuclear pore association
    Mathieu Rougemaille
    LEA Laboratory of Nuclear RNA Metabolism, Centre de Genetique Moleculaire, Centre National de la Recherche Scientifique UPR2167, 1, av de la Terrasse, 91190, Gif sur Yvette, France
    Cell 135:308-21. 2008
    ..Our data strongly suggests that the THO complex/Sub2p function is required to coordinate events leading to the acquisition of export competence at a step that follows commitment to 3'-processing...
  43. ncbi Termination of cryptic unstable transcripts is directed by yeast RNA-binding proteins Nrd1 and Nab3
    John T Arigo
    Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, 725 North Wolfe Street, Baltimore, Maryland 21205, USA
    Mol Cell 23:841-51. 2006
    ..These results suggest that transcription termination of CUTs directed by Nrd1 and Nab3 is a prerequisite for rapid degradation by the nuclear exosome...
  44. pmc Of proteins and RNA: the RNase P/MRP family
    Olga Esakova
    Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, Pennsylvania 16802, USA
    RNA 16:1725-47. 2010
    ..We review current information on RNase P and the closely related universal eukaryotic enzyme RNase MRP, focusing on their functions and structural organization...
  45. pmc La autoantigen is necessary for optimal function of the poliovirus and hepatitis C virus internal ribosome entry site in vivo and in vitro
    Mauro Costa-Mattioli
    Department of Biochemistry and McGill Cancer Center, McGill University, McIntyre Medical Building, Montreal, Quebec, Canada H3G 1Y6
    Mol Cell Biol 24:6861-70. 2004
    ..These results provide strong evidence for a function of the La autoantigen in IRES-dependent translation and define the step of translation which is stimulated by La...
  46. pmc RNA-binding proteins and post-transcriptional gene regulation
    Tina Glisovic
    Howard Hughes Medical Institute, Department of Biochemistry and Biophysics, University of Pennsylvania School of Medicine, Philadelphia, PA 19104 6148, United States
    FEBS Lett 582:1977-86. 2008
    ..In this short review, we focus on the RBPs that interact with pre-mRNAs and mRNAs and discuss their roles in the regulation of post-transcriptional gene expression...
  47. pmc Stress granules and processing bodies are dynamically linked sites of mRNP remodeling
    Nancy Kedersha
    Division of Rheumatology and Immunology, Harvard Medical School, Brigham and Women s Hospital, Boston, MA 02115, USA
    J Cell Biol 169:871-84. 2005
    ..We propose that mRNA released from disassembled polysomes is sorted and remodeled at SGs, from which selected transcripts are delivered to PBs for degradation...
  48. pmc Three-dimensional analysis of ribonucleoprotein complexes in influenza A virus
    Takeshi Noda
    Department of Special Pathogens, International Research Center for Infectious Diseases, Institute of Medical Science, University of Tokyo, Shirokanedai, Minato ku, Tokyo 108 8639, Japan
    Nat Commun 3:639. 2012
    ..Our data provide further insights into the mechanisms responsible for segmented-genome packaging into virions...
  49. doi Linking gene regulation to mRNA production and export
    Susana Rodriguez-Navarro
    Centro de Investigacion Principe Felipe, Valencia, Spain
    Curr Opin Cell Biol 23:302-9. 2011
    ..These include gene activation, transcription, mRNA processing and mRNP assembly and disassembly. In this review, we summarise recent insights into the links between these steps in the gene expression cascade...
  50. ncbi Host-range determinants on the PB2 protein of influenza A viruses control the interaction between the viral polymerase and nucleoprotein in human cells
    Karine Labadie
    Unite de Genetique Moleculaire des Virus Respiratoires, URA 1966 CNRS, EA302 Université Paris 7, Institut Pasteur, 25 rue du Dr Roux, 75724 Paris Cedex 15, France
    Virology 362:271-82. 2007
    The transcription/replication activity of ribonucleoproteins derived from influenza A primary isolates of human (A/Paris/908/97) or avian origin (A/Mallard/Marquenterre/MZ237/83, A/Hong Kong/156/97) was compared upon reconstitution in ..
  51. pmc Biochemical and structural evidence in support of a coherent model for the formation of the double-helical influenza A virus ribonucleoprotein
    Qiaozhen Ye
    Department of Biochemistry and Cell Biology, Rice University, Houston, Texas, USA
    MBio 4:e00467-12. 2012
    Influenza A virions contain eight ribonucleoproteins (RNPs), each comprised of a negative-strand viral RNA, the viral polymerase, and multiple nucleoproteins (NPs) that coat the viral RNA...
  52. ncbi Neurotrophin-induced transport of a beta-actin mRNP complex increases beta-actin levels and stimulates growth cone motility
    H L Zhang
    Department of Neuroscience, Rose F. Kennedy Center for Mental Retardation, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Neuron 31:261-75. 2001
    ..Growth cones exhibited impaired motility in the presense of antisense. These results suggest a novel mechanism to influence growth cone dynamics involving the regulated transport of mRNA...
  53. doi Clinical significance of anti-Ro52 (TRIM21) antibodies non-associated with anti-SSA 60kDa antibodies: results of a multicentric study
    P Ghillani
    Laboratoire d Immunochimie, Groupe Hospitalier Pitie Salpetriere, Paris, France
    Autoimmun Rev 10:509-13. 2011
    ..The presence of anti-Ro52 antibodies should probably precede development of autoimmune disease and must induce sequential follow-up of positive patients, particularly in interstitial lung disease progression...
  54. doi Akt phosphorylation of La regulates specific mRNA translation in glial progenitors
    F Brenet
    Department of Cancer Biology and Genetics, Memorial Sloan Kettering Cancer Center, New York, NY, USA
    Oncogene 28:128-39. 2009
    ..Therefore, La appears to be an important contributor to Akt-mediated translational regulation of these transcripts in murine glial cells...
  55. ncbi Cis-preferential LINE-1 reverse transcriptase activity in ribonucleoprotein particles
    Deanna A Kulpa
    Department of Human Genetics 1241 E Catherine St, University of Michigan Medical School, Ann Arbor, Michigan 48109 0618, USA
    Nat Struct Mol Biol 13:655-60. 2006
    ..We further demonstrate that ORF2p preferentially uses its encoding RNA as a template for reverse transcription. Thus, our data provide the first biochemical evidence supporting the cis-preferential action of the L1 reverse transcriptase...
  56. pmc Upf1 phosphorylation triggers translational repression during nonsense-mediated mRNA decay
    Olaf Isken
    Department of Biochemistry and Biophysics, School of Medicine and Dentistry, 601 Elmwood Avenue, Box 712, University of Rochester, Rochester, NY 14642, USA
    Cell 133:314-27. 2008
    ..Our results uncover a critical step during NMD that converts a pioneer translation initiation complex to a translationally compromised mRNP...
  57. pmc The small subunit processome in ribosome biogenesis—progress and prospects
    Kathleen R Phipps
    Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, CT, USA
    Wiley Interdiscip Rev RNA 2:1-21. 2011
    ..We will also explore how this knowledge of subcomplex assembly has informed our understanding of the workings of the ribosome synthesis system as a whole...
  58. ncbi Inhibition of nuclear export of ribonucleoprotein complexes of influenza virus by leptomycin B
    K Watanabe
    Laboratory of Molecular Medical Engineering, Department of Biological Information, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, 226-8501, Yokohama, Japan
    Virus Res 77:31-42. 2001
    ..e. LMB-sensitive and -insensitive NS2. The involvement of viral late proteins in vRNP export during late stages of infection is discussed...
  59. pmc Localization to, and effects of Pbp1, Pbp4, Lsm12, Dhh1, and Pab1 on stress granules in Saccharomyces cerevisiae
    Kylie D Swisher
    Department of Molecular and Cellular Biology, University of Arizona, Tucson, Arizona, USA
    PLoS ONE 5:e10006. 2010
    ..These observations offer new insight into the function of stress granule components with roles in stress granule assembly and mRNP regulation...
  60. doi Towards an atomic resolution understanding of the influenza virus replication machinery
    Rob W H Ruigrok
    Unit of Virus Host Cell Interactions, UJF EMBL CNRS, UMI 3265, Grenoble, France
    Curr Opin Struct Biol 20:104-13. 2010
    ..These results provide the first detailed structure-function insights into polymerase assembly, transcription and host adaptation and will have an impact on anti-influenza drug design...
  61. ncbi Factor required for mammalian spliceosome assembly is localized to discrete regions in the nucleus
    X D Fu
    Department of Biochemistry and Molecular Biology, Harvard University, Cambridge, Massachusetts 02138
    Nature 343:437-41. 1990
    ..These observations are evidence that spliceosome assembly could be compartmentalized within the nucleus...
  62. pmc Structural organization of a filamentous influenza A virus
    Lesley J Calder
    Division of Physical Biochemistry, MRC National Institute for Medical Research, London, UK
    Proc Natl Acad Sci U S A 107:10685-90. 2010
    ..The polar organization of the virus provides a model for assembly of the virion during budding at the host membrane. Images and tomograms of A/Aichi/68 X-31 virions show the generality of these conclusions to non-filamentous virions...
  63. pmc Influenza virus pleiomorphy characterized by cryoelectron tomography
    Audray Harris
    Laboratory of Structural Biology, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 103:19123-7. 2006
    ....
  64. ncbi Me31B silences translation of oocyte-localizing RNAs through the formation of cytoplasmic RNP complex during Drosophila oogenesis
    A Nakamura
    Institute of Biological Sciences, University of Tsukuba, Tsukuba, Ibaraki 305 8572, Japan
    Development 128:3233-42. 2001
    ..These results suggest that Me31B mediates translational silencing of RNAs during their transport to the oocyte. Our data provide evidence that RNA transport and translational control are linked through the assembly of RNP complex...
  65. ncbi The La protein
    Sandra L Wolin
    Departments of Cell Biology and Molecular Biophysics and Biochemistry, Howard Hughes Medical Institute, Yale University School of Medicine, 295 Congress Avenue, New Haven, Connecticut 06536, USA
    Annu Rev Biochem 71:375-403. 2002
    ..This review focuses on the roles of the La protein in small RNA biogenesis and also discusses data that implicate the La protein in the translation of specific mRNAs...
  66. doi The interface between transcription and mRNP export: from THO to THSC/TREX-2
    Ana G Rondon
    Centro Andaluz de Biologia Molecular y Medicina Regenerativa CABIMER, Universidad de Sevilla CSIC, Av Americo Vespucio s n, 41092 Sevilla, Spain
    Biochim Biophys Acta 1799:533-8. 2010
    ..Here we review our actual knowledge of the role that these factors play at the interface between transcription and mRNA export in the model organism Saccharomyces cerevisiae...
  67. ncbi Distinct pathways for snoRNA and mRNA termination
    Minkyu Kim
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Mol Cell 24:723-34. 2006
    ..These results suggest that in yeast the transcribing RNA polymerase II can choose between two distinct termination mechanisms but keeps both options available during elongation...
  68. ncbi Local activation of yeast ASH1 mRNA translation through phosphorylation of Khd1p by the casein kinase Yck1p
    Nicolas Paquin
    Departement de Biochimie, Universite de Montreal, Montreal, QC H3C 3J7, Canada
    Mol Cell 26:795-809. 2007
    ..This study reveals how, by linking mRNA sorting and translational activation, Khd1p and Yck1p regulate the spatiotemporal expression of a cell fate determinant...
  69. pmc A functional RNAi screen links O-GlcNAc modification of ribosomal proteins to stress granule and processing body assembly
    Takbum Ohn
    Division of Rheumatology, Immunology and Allergy, Brigham and Women s Hospital, Harvard Medical School, 1 Jimmy Fund Way, Boston, Massachusetts 02115, USA
    Nat Cell Biol 10:1224-31. 2008
    ..O-GlcNAc modification of the translational machinery is required for aggregation of untranslated messenger ribonucleoproteins into SGs...
  70. ncbi Architecture of ribonucleoprotein complexes in influenza A virus particles
    Takeshi Noda
    Internal Research Center for Infectious Diseases, Institute of Medical Science, University of Tokyo, Shirokanedai, Minato ku, Tokyo 108 8639, Japan
    Nature 439:490-2. 2006
    ..A selective mechanism of RNP incorporation into virions and the unique organization of the eight RNP segments may be crucial to maintaining the integrity of the viral genome during repeated cycles of replication...
  71. pmc Conservation of the protein composition and electron microscopy structure of Drosophila melanogaster and human spliceosomal complexes
    Nadine Herold
    Department of Cellular Biochemistry, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    Mol Cell Biol 29:281-301. 2009
    ..These studies provide insights into the evolutionarily conserved composition and structure of the metazoan spliceosome, as well as its compositional dynamics during catalytic activation...
  72. pmc Stable mRNP formation and export require cotranscriptional recruitment of the mRNA export factors Yra1p and Sub2p by Hpr1p
    Daniel Zenklusen
    Institute of Microbiology, University of Lausanne, 1011 Lausanne, Switzerland
    Mol Cell Biol 22:8241-53. 2002
    ..The data support the hypothesis that an exosome-dependent surveillance mechanism targets improperly assembled mRNPs for degradation...
  73. pmc The RNA-binding protein TbDRBD3 regulates the stability of a specific subset of mRNAs in trypanosomes
    Antonio M Estevez
    Instituto de Parasitologia y Biomedicina Lopez Neyra, CSIC Avda del Conocimiento s n, Armilla, 18100 Granada, Spain
    Nucleic Acids Res 36:4573-86. 2008
    ..These observations raise the possibility that TbDRBD3-mRNA complexes act as a post-transcriptional operon, and provide a framework to interpret how trypanosomes regulate gene expression in the absence of transcriptional control...
  74. ncbi Influenza virus propagation is impaired by inhibition of the Raf/MEK/ERK signalling cascade
    S Pleschka
    , , , D-35392 Giessen, Germany
    Nat Cell Biol 3:301-5. 2001
    ..Thus, signalling through the mitogenic cascade seems to be essential for virus production and RNP export from the nucleus during the viral life cycle...
  75. pmc Upf1 senses 3'UTR length to potentiate mRNA decay
    J Robert Hogg
    Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032, USA
    Cell 143:379-89. 2010
    ..From initial studies using RNA hairpin-tagged mRNAs for purification of messenger ribonucleoproteins assembled on transcripts with HIV-1 3' untranslated region (3'UTR) sequences, we uncover a two-step ..
  76. doi New clues to understand the role of THO and other functionally related factors in mRNP biogenesis
    Rosa Luna
    Centro Andaluz de Biologia Molecular y Medicina Regenerativa CABIMER, Universidad de Sevilla CSIC, Av Americo Vespucio s n, 41092 Sevilla, Spain
    Biochim Biophys Acta 1819:514-20. 2012
    ..This article is part of a Special Issue entitled: Nuclear Transport and RNA Processing...
  77. doi TRIM21 is essential to sustain IFN regulatory factor 3 activation during antiviral response
    Kai Yang
    Laboratory of Molecular Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China
    J Immunol 182:3782-92. 2009
    ....
  78. ncbi From birth to death: the complex lives of eukaryotic mRNAs
    Melissa J Moore
    Department of Biochemistry, Howard Hughes Medical Institute, Brandeis University, 415 South Street, Waltham, MA 02454
    Science 309:1514-8. 2005
    ..Thus, a comprehensive understanding of eukaryotic gene expression requires an appreciation for how the lives of mRNAs are influenced by a wide array of diverse regulatory mechanisms...
  79. ncbi Calreticulin inhibits repetitive intracellular Ca2+ waves
    P Camacho
    Department of Neuroscience, University of Virginia Health Sciences Center, Charlottesville 22908, USA
    Cell 82:765-71. 1995
    ..This novel function of CRT in intracellular Ca2+ signaling may be regulated by Ca2+ occupancy of the high affinity binding site...
  80. pmc The zipcode-binding protein ZBP1 influences the subcellular location of the Ro 60-kDa autoantigen and the noncoding Y3 RNA
    Soyeong Sim
    Department of Cell Biology, Yale University School of Medicine, New Haven, Connecticut 06536, USA
    RNA 18:100-10. 2012
    ..Both Ro and Y3 RNA accumulate in nuclei when ZBP1 is depleted. Our data indicate that ZBP1 may function as an adapter to export the Ro/Y3 RNA complex from nuclei...
  81. ncbi BCR/ABL activates mdm2 mRNA translation via the La antigen
    Rossana Trotta
    Department of Microbiology and Immunology, Kimmel Cancer Center, Thomas Jefferson Medical College, Philadelphia, PA 19107, USA
    Cancer Cell 3:145-60. 2003
    ..By contrast, La overexpression led to increased MDM2 levels and enhanced resistance to apoptosis. Thus, La-dependent activation of mdm2 translation might represent an important molecular mechanism involved in BCR/ABL leukemogenesis...
  82. ncbi U2AF65 recruits a novel human DEAD box protein required for the U2 snRNP-branchpoint interaction
    J Fleckner
    Howard Hughes Medical Institute, Program in Molecular Medicine, University of Massachusetts Medical Center, Worcester 01605, USA
    Genes Dev 11:1864-72. 1997
    ..Our results reveal a new function of U2AF65: to position a DEAD box protein required for U2 snRNP binding at the pre-mRNA branchpoint region...
  83. pmc The RNA polymerase II C-terminal domain promotes splicing activation through recruitment of a U2AF65-Prp19 complex
    Charles J David
    Department of Biological Sciences, Columbia University, New York, New York 10027, USA
    Genes Dev 25:972-83. 2011
    ..Our results not only provide a mechanism by which the CTD enhances splicing, but also describe unexpected interactions important for splicing and its coupling to transcription...
  84. pmc Differential recognition of the polypyrimidine-tract by the general splicing factor U2AF65 and the splicing repressor sex-lethal
    R Singh
    Department of Molecular, Cellular, and Developmental Biology, University of Colorado at Boulder, 80309, USA
    RNA 6:901-11. 2000
    ..The different dependence on individual contacts and nucleotide preference may provide a basis for the different RNA-binding specificities and thus functions of U2AF65 and SXL in 3' splice site choice...
  85. pmc Phosphorylation of S776 and 14-3-3 binding modulate ataxin-1 interaction with splicing factors
    Cesira de Chiara
    National Institute for Medical Research, Medical Research Council, London, United Kingdom
    PLoS ONE 4:e8372. 2009
    ..Studies of the interactions formed by non-expanded Atx1 thus provide valuable hints for understanding both the function of the non-pathologic protein and the causes of the disease...
  86. pmc Identification of a (CUG)n triplet repeat RNA-binding protein and its expression in myotonic dystrophy
    L T Timchenko
    Department of Medicine, Baylor College of Medicine, Houston, TX 77030, USA
    Nucleic Acids Res 24:4407-14. 1996
    ..We propose that the (CUG)n repeat region in Mt-PK mRNA is a binding site for CUG-BP/hNab50 in vivo, and triplet repeat expansion leads to sequestration of this hnRNP on mutant Mt-PK transcripts...
  87. pmc The splicing factor SC35 has an active role in transcriptional elongation
    Shengrong Lin
    Department of Cellular and Molecular Medicine, University of California, San Diego, 9500 Gilman Drive, La Jolla, California 92093 0651, USA
    Nat Struct Mol Biol 15:819-26. 2008
    ..Recombinant SC35 is sufficient to rescue this defect in nuclear run-on experiments. These findings suggest a reciprocal functional relationship between the transcription and splicing machineries during gene expression...
  88. pmc Structural basis for polypyrimidine tract recognition by the essential pre-mRNA splicing factor U2AF65
    E Allen Sickmier
    Department of Biochemistry and Molecular Biology, Johns Hopkins University Bloomberg School of Public Health, Baltimore, Maryland 21205, USA
    Mol Cell 23:49-59. 2006
    ..The energetic importance of conserved residues for Py tract binding is established by analysis of site-directed mutant U2AF(65) proteins using surface plasmon resonance...
  89. ncbi Control of transfer RNA maturation by phosphorylation of the human La antigen on serine 366
    R V Intine
    Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA
    Mol Cell 6:339-48. 2000
    ..Faithful phosphorylation of hLa on serine 366 reverses this block and promotes tRNA maturation. The results suggest that regulation of tRNA maturation at the level of RNase P cleavage may occur via phosphorylation of serine 366 of hLa...
  90. ncbi La protein binding at the GCAC site near the initiator AUG facilitates the ribosomal assembly on the hepatitis C virus RNA to influence internal ribosome entry site-mediated translation
    Renuka Pudi
    Department of Microbiology and Cell Biology, Indian Institute of Science, Sir C V Raman Avenue, Bangalore 560012, India
    J Biol Chem 279:29879-88. 2004
    ....
  91. pmc Genome instability and transcription elongation impairment in human cells depleted of THO/TREX
    María S Domínguez-Sánchez
    Centro Andaluz de Biologia Molecular y Medicina Regenerativa CABIMER, Universidad de Sevilla Consejo Superior de Investigaciones Cientificas CSIC, Sevilla, Spain
    PLoS Genet 7:e1002386. 2011
    ....
  92. ncbi Exonic splicing enhancers: mechanism of action, diversity and role in human genetic diseases
    B J Blencowe
    Dept of Medical Research, C H Best Institute, University of Toronto, 112 College St, Toronto, Ontario, Canada M5G 1L6
    Trends Biochem Sci 25:106-10. 2000
    ..Ominously, these latter studies predict that many human genetic diseases linked to mutations within exons might be caused by the inactivation of ESEs...
  93. pmc The influenza virus RNA synthesis machine: advances in its structure and function
    Patricia Resa-Infante
    Centro Nacional de Biotecnologia CSIC, Campus de Cantoblanco, Madrid, Spain
    RNA Biol 8:207-15. 2011
    ..and on the mechanisms by which they express their genetic information as mRNAs and generate progeny ribonucleoproteins that will become incorporated into new infectious virions...
  94. pmc PRORP proteins support RNase P activity in both organelles and the nucleus in Arabidopsis
    Bernard Gutmann
    Institut de Biologie Moleculaire des Plantes du CNRS, University of Strasbourg, 67084 Strasbourg, France
    Genes Dev 26:1022-7. 2012
    ..RNase Ps were believed until now to occur universally as ribonucleoproteins in organisms performing RNase P activity...
  95. pmc Cotranscriptional recruitment of the mRNA export factor Yra1 by direct interaction with the 3' end processing factor Pcf11
    Sara Ann Johnson
    Department of Biochemistry and Molecular Genetics, University of Colorado School of Medicine, Aurora, CO 80045, USA
    Mol Cell 33:215-26. 2009
    ..These results suggest a mechanism for cotranscriptional assembly of the export competent mRNP and for coordinating export with 3' end processing...
  96. pmc Sem1 is a functional component of the nuclear pore complex-associated messenger RNA export machinery
    Marius Boulos Faza
    Institute of Biochemistry, ETH Zurich, Zurich, Switzerland
    J Cell Biol 184:833-46. 2009
    ..Thus, Sem1 is a versatile protein that might stabilize multiple protein complexes involved in diverse pathways...
  97. pmc AU-rich-element-mediated upregulation of translation by FXR1 and Argonaute 2
    Shobha Vasudevan
    Department of Molecular Biophysics and Biochemistry, Howard Hughes Medical Institute, Yale University School of Medicine, New Haven, CT 06536, USA
    Cell 128:1105-18. 2007
    ..This novel cell-growth-dependent translation activation role for FXR1 and AGO2 allows new insights into ARE-mediated signaling and connects two important posttranscriptional regulatory systems in an unexpected way...
  98. ncbi Crystal structure of the rabies virus nucleoprotein-RNA complex
    Aurélie A V Albertini
    Institut de Virologie Moléculaire et Structurale, FRE 2854 Université Joseph Fourier CNRS, Boite Postale 181, 38042 Grenoble, France
    Science 313:360-3. 2006
    ....
  99. pmc Nuclear export dynamics of RNA-protein complexes
    DAVID GRUNWALD
    Delft University of Technology, Faculty of Applied Sciences, Kavli Institute of Nanoscience, Department of BioNanoScience, 2628 CJ Delft, The Netherlands
    Nature 475:333-41. 2011
    ..This review addresses the latest studies of RNA export and presents some models for how this complex process may work...
  100. pmc Structural basis for telomerase RNA recognition and RNP assembly by the holoenzyme La family protein p65
    Mahavir Singh
    Department of Chemistry, and the Molecular Biology Institute, University of California, Los Angeles, Los Angeles, CA 90095 1569, USA
    Mol Cell 47:16-26. 2012
    ..Additionally, our studies define a structurally homologous domain (xRRM) in genuine La and LARP7 proteins and suggest a general mode of RNA binding for biogenesis of their diverse RNA targets...
  101. pmc Emerging roles for the influenza A virus nuclear export protein (NEP)
    Duncan Paterson
    Sir William Dunn School of Pathology, University of Oxford, Oxford, United Kingdom
    PLoS Pathog 8:e1003019. 2012
    ..Accordingly, NEP is proposed to play multiple biologically important roles during the influenza virus life cycle...

Research Grants71

  1. Vitamin D and Estrogen Action in Bone: Concerted Role for hnRNPs in the C Family
    John S Adams; Fiscal Year: 2013
    ....
  2. Maternal Autoantibodies: Pathogenesis of Neonatal Lupus
    JILL PAMELA BUYON; Fiscal Year: 2013
    ..the mother, who may have a rheumatic disease or be asymptomatic, will have autoantibodies (Ab) to SSA/Ro ribonucleoproteins. CHB, a pathologic readout of passively acquired autoimmunity, provides an exceptional opportunity to ..
  3. Herpesvirus-induced telomerase dysregulation and tumor formation
    Keith William Jarosinski; Fiscal Year: 2011
    ..It will also address the mechanism of herpesvirus integration into the host genome and its importance for latent infection and transformation of lymphocytes. ..
  4. High-affinity RNA targets of Survival Motor Neuron Protein
    Ravindra N Singh; Fiscal Year: 2013
    ..Among several important functions, SMN has been implicated in biogenesis of small-nuclear ribonucleoproteins (snRNPs), transcription, pre-mRNA splicing, macromolecular trafficking, signal transduction and stress ..
  5. Hepatitis C virus and liver fibrogenesis
    Gulam Waris; Fiscal Year: 2010
    ..The information resulting from these investigations has a potential to provide clues to how HCV-induced oxidative stress and calcium signaling may induce profibrogenic factor, TGF-21, liver fibrosis and ultimately liver cancer. ..
  6. Noncoding RNA targets of the spinal muscular atrophy protein
    Livio Pellizzoni; Fiscal Year: 2010
    ..These studies have the potential to provide unanticipated insights into the molecular mechanisms of SMA and to suggest new avenues of therapeutic intervention. ..
  7. MOLECULAR MIMICRY IN IMMUNE MEDIATED NEUROLOGIC DISEASE
    Michael Levin; Fiscal Year: 2013
    ..The combination of both a diagnostic and pathogenic immune reaction is of particular relevance and has the potential to make important contributions to the long- term care and outcomes of people with MS. ..
  8. SPLICING OF MRNA PRECURSORS
    Michael R Green; Fiscal Year: 2013
    ....
  9. MOLECULAR MODELS OF IMMUNE-MEDIATED VASCULAR INJURY
    Jeffrey R Bender; Fiscal Year: 2012
    ....
  10. Defining the telomerase holoenzyme in progenitor cells with aging
    Steven E Artandi; Fiscal Year: 2013
    ..An improved understanding of telomerase is crucial for designing therapies that will delay or reverse certain aspects of aging. ..
  11. Role of dyskerin in oral epithelial homeostasis
    Faizan Alawi; Fiscal Year: 2013
    ..However, the mechanisms remain to be elucidated. Dyskerin is required for the biogenesis of ribonucleoproteins that incorporate small non-coding RNA molecules characterized by the H/ACA secondary structure...
  12. The survival of motor neuron protein in axonal mRNA localization
    PAUL GREGORY DONLIN-ASP; Fiscal Year: 2013
    ..Through identification of novel mislocalized mRNA transcripts and a role for SMN in mRNA localization, this research will identify novel targets for the development of SMA therapies. ..
  13. The influenza A virus nucleoprotein: structure and function
    Yizhi Jane Tao; Fiscal Year: 2012
    ..The aim of this grant is to elucidate the molecular mechanism and biological significance of several key activities of the influenza A virus nucleoprotein and to discover how such activities can be exploited for antiviral development. ..
  14. Post Translational Modifications in Tolerance and Autoimmunity
    Mark J Mamula; Fiscal Year: 2012
    ..The goal of this work is to determine the importance of spontaneous protein modification in B and T cell immune tolerance and autoimmune pathology. ..
  15. Structure and mechanism of CRISPR interference.
    Ailong Ke; Fiscal Year: 2013
    ..Our findings will serve to reveal the common theme and mechanistic diversity among different CRISPR-Cas systems. ..
  16. Regulation of Endothelial Nitric Oxide Synthase mRNA stability
    Jianxin Sun; Fiscal Year: 2013
    ..the proposed study may well provide significant insights into the molecular mechanisms underlying these conditions and thus facilitate the development of novel therapeutic agents. ..
  17. SMN Control of snRNP Biogenesis: Role in RNA Splicing and Motor Neuron Survival
    Livio Pellizzoni; Fiscal Year: 2013
    ..SMN has a well-established function in the assembly of small nuclear ribonucleoproteins (snRNPs), which are the essential components of the splicing machinery...
  18. Group II Intron Mobility and Gene Targeting
    Alan M Lambowitz; Fiscal Year: 2012
    ....
  19. Regulation and Function of Germline RNP Granules
    JENNIFER SCHISA; Fiscal Year: 2010
    ..This research tests the hypothesis that large RNP granules play a protective role in maintaining oocyte viability and fertility and may be relevant to understanding the basis for infertility that occurs as women age. ..
  20. Defining the messenger RNP code in the brain
    EUGENE WEI MING YEO; Fiscal Year: 2013
    ..Finally, we will leverage our computational expertise to build predictive models using this genome-wide, multi-scale, mRNP code in the brain. ..
  21. The regulation and function of cytoplasmic foci in quiescent cells
    Paul K Herman; Fiscal Year: 2013
    ..The specific aims of this proposal are: (1) to determine the role of PKA signaling in the regulation of P-body foci formation;and (2) to determine the physiological role of P-body foci in quiescent cells. ..
  22. Analyses of two RNA helicases that affect intercellular transport via plasmodesma
    Patricia C Zambryski; Fiscal Year: 2012
    ....
  23. Production and Evaluation of a Vault-CCL21 Nanocapsule as a Lung Cancer Treatment
    GEORGE BUCHMAN; Fiscal Year: 2012
    ..This is a potentially disruptive approach that can revolutionize the lung cancer field, and may be applied to other cancers. ..
  24. Structure and dynamics of the Tetrahymena telomerase ribonucleoprotein
    Michael D Stone; Fiscal Year: 2013
    ..To this end, this proposal aims to illuminate the structural properties of telomerase that underlie its unique cellular activity. ..
  25. Preventive Approach to Congenital Heart Block with Hydroxychloroquine (PATCH)
    JILL PAMELA BUYON; Fiscal Year: 2013
    ..A positive result will likely change the management of all anti-Ro positive women who have had a previous child with CHB. Furthermore, potential prevention would justify screening of all pregnant women for anti-Ro antibodies. ..
  26. Translational regulation of cellular morphogenesis in early Drosophila embryos
    Paul M Macdonald; Fiscal Year: 2012
    ....
  27. Control of Oocyte Maturation in C. elegans
    DAVID IRWIN GREENSTEIN; Fiscal Year: 2013
    ..We will first identify mRNAs in OMA- ribonucleoproteins using RNA-binding protein immunoprecipitation-microarray profiling...
  28. Definition of Serum Ribonucleoprotein Composition and its Regulation and Function
    Thomas Tuschl; Fiscal Year: 2013
    ..Identify the RNA targets and RNP structures of autoantigens in tRNA stress responses, their turnover, and their immune receptors. ..
  29. MOLECULAR GENETICS OF DOSAGE COMPENSATION IN DROSOPHILA
    MITZI KURODA; Fiscal Year: 2013
    ..abstract_text> ..
  30. B CELL HYPERACTIVITY IN AUTOIMMUNITY
    Ann Marshak Rothstein; Fiscal Year: 2010
    ..by the excessive production of a wide range of autoantibodies specific for self antigens such as chromatin, ribonucleoproteins and phospholipids...
  31. Nutritional Regulation of Leptin Production
    Susan K Fried; Fiscal Year: 2010
    ....
  32. Regulation of CD45 alternative splicing by HNRPLL
    Anjana Rao; Fiscal Year: 2013
    ....
  33. Dosage Compensation in Drosophila: Establishing Coordinate Gene Regulation
    MARCELA SORUCO; Fiscal Year: 2013
    ..We expect that defining how genes are coordinately regulated will provide key therapeutic targets for diverse diseases. ..
  34. Immune Response to Small Nuclear Ribonucleoprotein Autoantigens
    Eric L Greidinger; Fiscal Year: 2011
    ..Our research goal is to develop new and effective treatments of these disorders. ..
  35. Translation Regulation of Collagen-1 in Liver Fibrosis: Target of Drug Discovery
    Zarko Manojlovic; Fiscal Year: 2013
    ....
  36. Small Regulatory RNA Functions in the Nucleus
    Scott G Kennedy; Fiscal Year: 2012
    ..Our research is addressing these questions. ..
  37. REGULATION OF RETROTRANSPOSITION IN S. CEREVISIAE
    M Joan Curcio; Fiscal Year: 2013
    ..Cellular proteins that are required for retroviral replication are potential targets for the development of new drugs to treat AIDS. ..
  38. Connexin Distribution in Physiological Versus Pathological Cardiac Hypertrophy
    Michael R Zile; Fiscal Year: 2013
    ..pathological hypertrophy, with ex- tensively characterized cytoskeletal properties in each setting. ..
  39. Micro-RNA 122 and Chronic Hepatitis C
    Stanley M Lemon; Fiscal Year: 2013
    ..The studies proposed will provide novel insight into how it functions in HCV replication and in the pathogenesis of chronic hepatitis C, thereby enhancing the scientific background on which it can be exploited as a therapeutic target. ..
  40. Role of Lipids in Hepatitis C Virus Maturation
    Aleem Siddiqui; Fiscal Year: 2013
    ..These studies will reveal unique insights into the mechanisms of HCV maturation, secretion and budding processes and provide a unique model for other RNA viruses. ..
  41. Structural insights into pandemic and emerging influenza viruses
    Ian A Wilson; Fiscal Year: 2013
    ....
  42. ROLE OF ACA11, AN ORPHAN SMALL NUCLEOLAR RNA IN RESISTANCE TO CANCER CHEMOTHERAPY
    Jason Weber; Fiscal Year: 2013
    ..regulates ROS levels through modulating splicing of snoRNAs from ribosomal protein transcripts Aim 3: To test the hypothesis that overexpression of ACA11 leads to an inhibition of nucleolar stress-induced, p53-regulated ROS production ..
  43. Genetic Regulation of Complex Neurological Diseases
    Wayne N Frankel; Fiscal Year: 2013
    ..In parallel, we will explore whether CELF4 has a coordinating role in shaping cellular responses by examining mutant cell culture, acute brain slice and whole animal models of neuronal plasticity. ..
  44. Mechanism of Folate Deficiency as a Co-Factor for HPV16-induced Carcinogenesis
    ASOK ANTONY; Fiscal Year: 2013
    ..nutrition;Veterans with HPV16 and human immunodeficiency virus (HIV);and those in developing countries where the combination of poor nutrition, and co-infection with HIV and HPV16 places individuals at high risk for HPV-induced cancers ..
  45. Ty3 viruslike particle morphogenesis and host interactions
    Suzanne Sandmeyer; Fiscal Year: 2013
    ..Huang (UCI) to map interactions within and between cellular VLP, PB, and NPC. The Baldi group (UCI) has expertise in DNA-seq analysis and protein modeling and will collaborate on several aspects of this work. ..
  46. Cell Cycle Regulation of Yeast Telomerase Assembly and Function
    KATHERINE LOUISE FRIEDMAN; Fiscal Year: 2012
    ..By examining the mechanisms that regulate telomerase activity, we hope to identify novel targets for anti-tumor therapy. ..
  47. Investigating the Role of the Est1p in Telomerase Biogenesis and Regulation
    Charlene Hawkins; Fiscal Year: 2012
    ....
  48. Proteins in RNA-Based Enzymes
    Andrey S Krasilnikov; Fiscal Year: 2012
    ....
  49. Development of RIP-Chip methods and tiled arrays to identify functional elements
    Michael L Whitfield; Fiscal Year: 2010
    ..Understanding the regulatory code that directs the expression of genes at all levels (transcription, pre-mRNA processing, translational control and RNA stability) will provide a better understanding of this basic process. ..
  50. The role of the SF3B1 splicing factor in chronic lymphocytic leukemia
    CATHERINE JU YING WU; Fiscal Year: 2013
    ....
  51. MECHANISMS FOR REARRANGING RNA DURING PRE-MRNA SPLICING
    Jonathan P Staley; Fiscal Year: 2013
    ..A major goal of our work is to determine how errors in this process are prevented to better understand the etiology of disease and how to treat disease. ..
  52. The Pathogenesis of Dyskeratosis Congenita
    Philip J Mason; Fiscal Year: 2013
    ....
  53. Biogenesis and Regulation of Human Telomerase
    Kathleen Collins; Fiscal Year: 2013
    ..The long-term goal of these studies is to understand telomerase RNP assembly, catalytic activation, and cellular regulation in normal cells and disease and to exploit this understanding for improvement of human health. ..
  54. Biogenesis of Small Ribonucleoproteins
    A Gregory Matera; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Small ribonucleoproteins (RNPs) are essential components of all cells...
  55. Mechanisms of CRISPR Interference
    Erik J Sontheimer; Fiscal Year: 2013
    ..lie outside of the cas locus) that are required for interference;and (iii) characterize crRNA-containing ribonucleoproteins (crRNPs) and define their properties, components, activities, and precursor-product relationships...
  56. Structural Studies of Telomerase Holoenzyme
    EDWARD JOHN MIRACCO; Fiscal Year: 2013
    ..These experiments will reveal the complete structure of a telomerase holoenzyme as well as offer insight into what roles intermolecular contacts and subunit movement play in telomere catalysis. ..
  57. Crohn's disease-associated NOD2 Mutants
    Xiaojing Ma; Fiscal Year: 2010
    ..We propose to investigate the mechanisms whereby R702W and G908R regulate IL-12/IL-23 gene expression. ..
  58. Developing in vivo and in vitro Models for ALS Caused by Mutant FUS
    Xugang Xia; Fiscal Year: 2011
    ..The resulting rat model would be useful to mechanistic study of the disease and could be suitable for testing potential therapies for this devastating disease, having a direct impact on patient health. ..
  59. Eukaryotic Telomere Dynamics
    Arthur J Lustig; Fiscal Year: 2013
    ....
  60. Structure and Function of Human Telomerase
    JULI F FEIGON; Fiscal Year: 2013
    ..The results of these investigations will provide a molecular basis for understanding telomerase activity as well as how mutations linked to disease affect activity, and for designing drugs that target TR. ..
  61. Regulation of AU-rich element-mediated mRNA decay
    Ching Yi Chen; Fiscal Year: 2013
    ..Understanding the regulation of mRNA degradation would provide insights into the disease pathogenesis. Our research should allow for the development of alternative treatments for immune and inflammatory diseases and cancer. ..
  62. Beagle: A Supercomputer for Computational Biology, Simulation, and Data Analysis
    Ian Foster; Fiscal Year: 2010
    ..substantial support from the University of Chicago for operations and application support, and an extremely experienced technical staff allows us to propose a system with capabilities unprecedented in academic biomedicine. ..
  63. TGF-regulated EMT
    Philip H Howe; Fiscal Year: 2013
    ....
  64. NUCLEAR ACIDIC PROTEIN ANTIGENS IN RHEUMATIC DISEASES
    GORDON SHARP; Fiscal Year: 1990
    ..The product of autoimmune response, the immune complex, will be biochemically characterized and its distribution studied for specific autoantibody-antigen reactivities...
  65. CONTROL OF CELL FUNCTION VIA SELECTIVE MRNA TRANSLATION
    Lee Gehrke; Fiscal Year: 2002
    ..Several approaches for high resolution structural analysis are proposed, including NMR and X-ray crystallography of chimeric virus particles expressing surface RNA- binding peptides. ..
  66. Crystallizing Hepatitis B Virus Reverse Transcriptase
    Hwai Chen Guo; Fiscal Year: 2006
    ..abstract_text> ..
  67. STRUCTURAL AND FUNCTIONAL ANALYSIS OF TELOMERASE RNAS
    Daniel Romero; Fiscal Year: 1999
    ..Telomerase will be partially purified from transformants expressing mutant TER and characterized more completely with respect to number of kinetic parameters (substrate and dNTP binding, Vmax, etc.) ..
  68. "Analyses of two genes that affect plasmodesmata structure and function"
    Patricia Zambryski; Fiscal Year: 2007
    ..Our lab is uniquely poised to carry out the above experiments that will significantly impact our understanding of these influential channels. ..
  69. Immune Recognition of Modified Antigen in Scleroderma
    Robert Hoffman; Fiscal Year: 2003
    Small nuclear ribonucleoproteins (snRNP) are prominent self antigens targeted in scleroderma and other autoimmune conditions...
  70. Activation of Innate Immunity by Small Ribonucleoprotein
    Westley Reeves; Fiscal Year: 2007
    ..lupus erythematosus (SLE) develop autoantibodies to ubiquitous host cellular molecules, such as Sm small ribonucleoproteins (snRNPs) containing the small RNAs U1, U2, U4-6, and U5 RNAs...
  71. Role of Ca channels in congenital heart block
    Mohamed Boutjdir; Fiscal Year: 2007
    ..that affects fetuses and/or newborn of mothers with autoantibodies reactive with the intracellular soluble ribonucleoproteins 48kD SSB/La, 52kD SSA/Ro, and 60kD SSA/Ro...