aquaporins

Summary

Summary: A class of porins that allow the passage of WATER and other small molecules across CELL MEMBRANES.

Top Publications

  1. pmc The zebrafish genome encodes the largest vertebrate repertoire of functional aquaporins with dual paralogy and substrate specificities similar to mammals
    Angèle Tingaud-Sequeira
    Laboratory of Institut de Recerca i Tecnologia Agroalimentàries Institut de Ciències del Mar, Consejo Superior de Investigaciones Cientificas, 08003 Barcelona, Spain
    BMC Evol Biol 10:38. 2010
  2. ncbi Specific aquaporins facilitate the diffusion of hydrogen peroxide across membranes
    Gerd P Bienert
    Department of Agricultural Sciences, Faculty of Life Science, Copenhagen University, DK 1871 Frederiksberg C, Denmark
    J Biol Chem 282:1183-92. 2007
  3. ncbi Structural determinants of water permeation through aquaporin-1
    K Murata
    National Institute for Physiological Sciences, Okazaki, Japan
    Nature 407:599-605. 2000
  4. ncbi Impairment of angiogenesis and cell migration by targeted aquaporin-1 gene disruption
    Samira Saadoun
    Department of Medicine, Cardiovascular Research Institute, University of California, San Francisco, San Francisco, California 94143, USA
    Nature 434:786-92. 2005
  5. pmc Aquaporins in clinical medicine
    A S Verkman
    Department of Medicine, University of California, San Francisco, California 94143 0521, USA
    Annu Rev Med 63:303-16. 2012
  6. pmc The complete set of genes encoding major intrinsic proteins in Arabidopsis provides a framework for a new nomenclature for major intrinsic proteins in plants
    U Johanson
    Department of Plant Biochemistry, Lund University, P O Box 117, SE 221 00 Lund, Sweden
    Plant Physiol 126:1358-69. 2001
  7. pmc Early effects of salinity on water transport in Arabidopsis roots. Molecular and cellular features of aquaporin expression
    Yann Boursiac
    Biochimie et Physiologie Moleculaire des Plantes, Agro Montpellier Centre National de la Recherche Scientifique Institut National de la Recheche Agonomique Université Montpellier 2, Unité Mixte de Recherche 5004, France
    Plant Physiol 139:790-805. 2005
  8. ncbi From structure to disease: the evolving tale of aquaporin biology
    Landon S King
    Department of Medicine, Division of Pulmonary and Critical Care Medicine, The Johns Hopkins University School of Medicine, 5501 Hopkins Bayview Circle, Baltimore, Maryland 21224, USA
    Nat Rev Mol Cell Biol 5:687-98. 2004
  9. ncbi Bundle-sheath cell regulation of xylem-mesophyll water transport via aquaporins under drought stress: a target of xylem-borne ABA?
    Arava Shatil-Cohen
    Faculty of Agriculture, Food and Environment, The Robert H Smith Institute of Plant Sciences and Genetics in Agriculture, The Hebrew University of Jerusalem, Rehovot 76100, Israel
    Plant J 67:72-80. 2011
  10. ncbi Fluid transport across leaky epithelia: central role of the tight junction and supporting role of aquaporins
    Jorge Fischbarg
    Institute of Cardiology Research, A C Taquini, University of Buenos Aires and National Council for Scientific and Technical Investigations, Buenos Aires, Argentina
    Physiol Rev 90:1271-90. 2010

Detail Information

Publications365 found, 100 shown here

  1. pmc The zebrafish genome encodes the largest vertebrate repertoire of functional aquaporins with dual paralogy and substrate specificities similar to mammals
    Angèle Tingaud-Sequeira
    Laboratory of Institut de Recerca i Tecnologia Agroalimentàries Institut de Ciències del Mar, Consejo Superior de Investigaciones Cientificas, 08003 Barcelona, Spain
    BMC Evol Biol 10:38. 2010
    b>Aquaporins are integral membrane proteins that facilitate the transport of water and small solutes across cell membranes. These proteins are vital for maintaining water homeostasis in living organisms...
  2. ncbi Specific aquaporins facilitate the diffusion of hydrogen peroxide across membranes
    Gerd P Bienert
    Department of Agricultural Sciences, Faculty of Life Science, Copenhagen University, DK 1871 Frederiksberg C, Denmark
    J Biol Chem 282:1183-92. 2007
    ..g. to cross membranes. Here we present evidence that aquaporins can channel hydrogen peroxide (H2O2)...
  3. ncbi Structural determinants of water permeation through aquaporin-1
    K Murata
    National Institute for Physiological Sciences, Okazaki, Japan
    Nature 407:599-605. 2000
    ..The atomic model provides a possible molecular explanation to a longstanding puzzle in physiology-how membranes can be freely permeable to water but impermeable to protons...
  4. ncbi Impairment of angiogenesis and cell migration by targeted aquaporin-1 gene disruption
    Samira Saadoun
    Department of Medicine, Cardiovascular Research Institute, University of California, San Francisco, San Francisco, California 94143, USA
    Nature 434:786-92. 2005
    ..Our findings support a fundamental role of water channels in cell migration, which is central to diverse biological phenomena including angiogenesis, wound healing, tumour spread and organ regeneration...
  5. pmc Aquaporins in clinical medicine
    A S Verkman
    Department of Medicine, University of California, San Francisco, California 94143 0521, USA
    Annu Rev Med 63:303-16. 2012
    The aquaporins are a family of membrane water channels, some of which also transport glycerol...
  6. pmc The complete set of genes encoding major intrinsic proteins in Arabidopsis provides a framework for a new nomenclature for major intrinsic proteins in plants
    U Johanson
    Department of Plant Biochemistry, Lund University, P O Box 117, SE 221 00 Lund, Sweden
    Plant Physiol 126:1358-69. 2001
    ..The complete set of AtMIPs, together with the new nomenclature, will facilitate the isolation, classification, and labeling of plant MIPs from other species...
  7. pmc Early effects of salinity on water transport in Arabidopsis roots. Molecular and cellular features of aquaporin expression
    Yann Boursiac
    Biochimie et Physiologie Moleculaire des Plantes, Agro Montpellier Centre National de la Recherche Scientifique Institut National de la Recheche Agonomique Université Montpellier 2, Unité Mixte de Recherche 5004, France
    Plant Physiol 139:790-805. 2005
    b>Aquaporins facilitate the uptake of soil water and mediate the regulation of root hydraulic conductivity (Lp(r)) in response to a large variety of environmental stresses...
  8. ncbi From structure to disease: the evolving tale of aquaporin biology
    Landon S King
    Department of Medicine, Division of Pulmonary and Critical Care Medicine, The Johns Hopkins University School of Medicine, 5501 Hopkins Bayview Circle, Baltimore, Maryland 21224, USA
    Nat Rev Mol Cell Biol 5:687-98. 2004
    ..has been greatly advanced by the discovery of a family of water-specific, membrane-channel proteins - the aquaporins. These proteins are present in organisms at all levels of life, and their unique permeability characteristics ..
  9. ncbi Bundle-sheath cell regulation of xylem-mesophyll water transport via aquaporins under drought stress: a target of xylem-borne ABA?
    Arava Shatil-Cohen
    Faculty of Agriculture, Food and Environment, The Robert H Smith Institute of Plant Sciences and Genetics in Agriculture, The Hebrew University of Jerusalem, Rehovot 76100, Israel
    Plant J 67:72-80. 2011
    ..the entire leaf vasculature) have been hypothesized to control K(leaf) via the specific activity of BSC aquaporins (AQPs)...
  10. ncbi Fluid transport across leaky epithelia: central role of the tight junction and supporting role of aquaporins
    Jorge Fischbarg
    Institute of Cardiology Research, A C Taquini, University of Buenos Aires and National Council for Scientific and Technical Investigations, Buenos Aires, Argentina
    Physiol Rev 90:1271-90. 2010
    ..These elements are remarkably similar to those proposed by the laboratory of Adrian Hill for fluid transport across other leaky epithelia...
  11. ncbi Whole gene family expression and drought stress regulation of aquaporins
    Erik Alexandersson
    Department of Plant Biochemistry, Lund University, PO Box 124, SE 22100, Lund, Sweden
    Plant Mol Biol 59:469-84. 2005
    Since many aquaporins (AQPs) act as water channels, they are thought to play an important role in plant water relations...
  12. ncbi The tobacco aquaporin NtAQP1 is a membrane CO2 pore with physiological functions
    Norbert Uehlein
    Institute of Botany, Schnittspahnstrasse 3, D 64287 Darmstadt, Germany
    Nature 425:734-7. 2003
    b>Aquaporins, found in virtually all living organisms, are membrane-intrinsic proteins that form water-permeable complexes...
  13. ncbi Aquaporin-4 in the central nervous system: cellular and subcellular distribution and coexpression with KIR4.1
    E A Nagelhus
    Nordic Centre of Excellence for Research in Water Imbalance Related Disorders and Centre for Molecular Biology and Neuroscience, Institute of Basic Medical Sciences, University of Oslo, POB 1105 Blindern, N 0317 Oslo, Norway
    Neuroscience 129:905-13. 2004
    ..We conclude that AQP4-mediated water flux represents an integral element of brain volume and ion homeostasis...
  14. ncbi Cellular and molecular biology of the aquaporin water channels
    M Borgnia
    Department of Biological Chemistry, Johns Hopkins School of Medicine, Baltimore, Maryland 21205 2185, USA
    Annu Rev Biochem 68:425-58. 1999
    ..Ten mammalian aquaporins have been identified in water-permeable tissues and fall into two groupings...
  15. pmc PIP1 plasma membrane aquaporins in tobacco: from cellular effects to function in plants
    Franka Siefritz
    Department of Plant Physiology and Biophysics, University of Wurzburg, D 97082 Wurzburg, Germany
    Plant Cell 14:869-76. 2002
    The molecular functions of several aquaporins are well characterized (e.g., by analysis of aquaporin-expressing Xenopus oocytes)...
  16. pmc Plasma membrane aquaporins play a significant role during recovery from water deficit
    Pierre Martre
    Department of Organismic Biology, Ecology, and Evolution, University of California, Los Angeles 90095 1606, USA
    Plant Physiol 130:2101-10. 2002
    The role of plasma membrane aquaporins (PIPs) in water relations of Arabidopsis was studied by examining plants with reduced expression of PIP1 and PIP2 aquaporins, produced by crossing two different antisense lines...
  17. pmc Structural context shapes the aquaporin selectivity filter
    David F Savage
    Graduate Group in Biophysics, Department of Biochemistry and Biophysics, University of California, San Francisco, CA 94158 2517, USA
    Proc Natl Acad Sci U S A 107:17164-9. 2010
    b>Aquaporins are transmembrane channels that facilitate the permeation of water and small, uncharged amphipathic molecules across cellular membranes...
  18. ncbi The lens circulation
    Richard T Mathias
    Department of Physiology and Biophysics, State University of New York at Stony Brook, Stony Brook, New York, NY 11794 8661, USA
    J Membr Biol 216:1-16. 2007
    ..This review covers the membrane transport systems that are believed to generate and direct this internal circulatory system...
  19. pmc Overexpression of a plasma membrane aquaporin in transgenic tobacco improves plant vigor under favorable growth conditions but not under drought or salt stress
    Refael Aharon
    Department of Plant Sciences, The Weizmann Institute of Science, Rehovot 76100, Israel
    Plant Cell 15:439-47. 2003
    Most of the symplastic water transport in plants occurs via aquaporins, but the extent to which aquaporins contribute to plant water status under favorable growth conditions and abiotic stress is not clear...
  20. pmc The role of plasma membrane intrinsic protein aquaporins in water transport through roots: diurnal and drought stress responses reveal different strategies between isohydric and anisohydric cultivars of grapevine
    Rebecca K Vandeleur
    School of Agriculture, Food, and Wine, University of Adelaide, Waite Campus, Urrbrae, South Australia 5064, Australia
    Plant Physiol 149:445-60. 2009
    ..Of the two most highly expressed plasma membrane intrinsic protein (PIP) aquaporins in roots (VvPIP1;1 and VvPIP2;2), only VvPIP2;2 functions as a water channel in Xenopus laevis oocytes...
  21. ncbi Expression and functional analysis of the rice plasma-membrane intrinsic protein gene family
    Lei Guo
    Peking Yale Joint Research Center for Plant Molecular Genetics and AgroBiotechnology, National Laboratory for Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Peking University, Beijing 100871, China
    Cell Res 16:277-86. 2006
    Plasma membrane intrinsic proteins (PIPs) are a subfamily of aquaporins that enable fast and controlled translocation of water across the membrane. In this study, we systematically identified and cloned ten PIP genes from rice...
  22. ncbi Role of aquaporins in leaf physiology
    Robert B Heinen
    Institut des Sciences de la Vie, Universite Catholique de Louvain, Croix du Sud 5 15, B 1348 Louvain la Neuve, Belgium
    J Exp Bot 60:2971-85. 2009
    ..Transcellular water flow is facilitated and regulated by water channels in the membranes, named aquaporins (AQPs)...
  23. ncbi Identification of 33 rice aquaporin genes and analysis of their expression and function
    Junko Sakurai
    CO2 and Temperature Research Laboratory, National Agricultural Research Center for Tohoku Region, Morioka, 020 0198 Japan
    Plant Cell Physiol 46:1568-77. 2005
    Plant aquaporins form a large protein family including plasma membrane-type (PIPs) and tonoplast-type aquaporins (TIPs), and facilitate osmotic water transport across membranes as a key physiological function...
  24. pmc Unexpected complexity of the aquaporin gene family in the moss Physcomitrella patens
    Jonas A H Danielson
    Department of Biochemistry, Center for Molecular Protein Science, Center for Chemistry and Chemical Engineering, Lund University, PO Box 124, S 221 00 Lund, Sweden
    BMC Plant Biol 8:45. 2008
    b>Aquaporins, also called major intrinsic proteins (MIPs), constitute an ancient superfamily of channel proteins that facilitate the transport of water and small solutes across cell membranes...
  25. ncbi The structure of aquaporins
    Tamir Gonen
    Department of Biochemistry, University of Washington, Seattle, WA, USA
    Q Rev Biophys 39:361-96. 2006
    ..The ubiquitous members of the aquaporin (AQP) family form transmembrane pores that are either exclusive for water (aquaporins) or are also permeable for other small neutral solutes such as glycerol (aquaglyceroporins)...
  26. ncbi Phylogeny and evolution of the major intrinsic protein family
    Rafael Zardoya
    Departamento de Biodiversidad y Biologia Evolutiva, Museo Nacional de Ciencias Naturales, CSIC, José Gutiérrez Abascal, 2, 28006 Madrid, Spain
    Biol Cell 97:397-414. 2005
    ..With the recent increase in genomic projects, there is a considerable increase in the quantity and taxonomic range of MIPs in molecular databases...
  27. ncbi Aquaporins in brain: distribution, physiology, and pathophysiology
    Jerome Badaut
    Departement de Neurochirurgie, Centre Hospitalier Universitaire Vaudois, Lausanne, Switzerland
    J Cereb Blood Flow Metab 22:367-78. 2002
    ..A water channel family, the aquaporins, facilitates water flux through the plasma membrane of many cell types...
  28. ncbi Water movements in the brain: role of aquaporins
    Matthew J Tait
    Academic Neurosurgery Unit, St George s University of London, Cranmer Terrace, Tooting, London SW17 0RE, UK
    Trends Neurosci 31:37-43. 2008
    ..AQP9 is present in some glia and neurons, but its function is unclear. Finally, we discuss how the discovery of AQP activators and inhibitors will be the next major step in this field...
  29. pmc Lipid-protein interactions in double-layered two-dimensional AQP0 crystals
    Tamir Gonen
    Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, Massachusetts 02115, USA
    Nature 438:633-8. 2005
    ..We were therefore able to build an atomic model for the lipid bilayer surrounding the AQP0 tetramers, and we describe lipid-protein interactions...
  30. ncbi Defective secretion of saliva in transgenic mice lacking aquaporin-5 water channels
    T Ma
    Departments of Medicine, Physiology, and Pediatrics, Cardiovascular Research Institute, University of California, San Francisco, California 94143 0521, USA
    J Biol Chem 274:20071-4. 1999
    ..These results implicate a key role for AQP5 in saliva fluid secretion and provide direct evidence that high epithelial cell membrane water permeability is required for active, near-isosmolar fluid transport...
  31. pmc Drought and abscisic acid effects on aquaporin content translate into changes in hydraulic conductivity and leaf growth rate: a trans-scale approach
    Boris Parent
    INRA, UMR 759 Laboratoire d Ecophysiologie des Plantes sous Stress Environnementaux, F 34060 Montpellier, France
    Plant Physiol 149:2000-12. 2009
    ..because it was nearly abolished by a hydrogen peroxide treatment, which blocks the water channel activity of aquaporins. The hydraulic conductance of intact whole plants was affected in the same way when measured either in steady-..
  32. ncbi Effect of overexpression of radish plasma membrane aquaporins on water-use efficiency, photosynthesis and growth of Eucalyptus trees
    Ayako Tsuchihira
    Laboratory of Cell Dynamics, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464 8601, Japan
    Tree Physiol 30:417-30. 2010
    ..Australia, compared their growth rate and drought tolerance and determined the protein levels of plasma membrane aquaporins (PIPs). There was a positive relationship between the drought tolerance and PIP content...
  33. ncbi Membrane transport of hydrogen peroxide
    Gerd P Bienert
    Plant and Soil Sciences Laboratory, Department of Agricultural Sciences, The Royal Veterinary and Agricultural University, Thorvaldsensvej 40, DK 1871 Frederiksberg C, Denmark
    Biochim Biophys Acta 1758:994-1003. 2006
    ..The main focus of this review is (i) to summarize the most recent evidence for a signalling role of H2O2 in various pathways in plants and mammals and (ii) to discuss the relevance of specific transport of H2O2...
  34. ncbi Overexpressing a putative aquaporin gene from wheat, TaNIP, enhances salt tolerance in transgenic Arabidopsis
    Zhenxian Gao
    College of Life Science of Hebei Normal University, No 113 Yuhua East Road, Shijiazhuang, Hebei, PR China
    Plant Cell Physiol 51:767-75. 2010
    ..Our results suggest that TaNIP plays an important role in salt tolerance in Arabidopsis and can also enhance plants' tolerance to other abiotic stresses...
  35. pmc Role of a single aquaporin isoform in root water uptake
    Hélène Javot
    Biochimie et Physiologie Moleculaire des Plantes, Agro M Centre National de la Recherche Scientifique Institut National de la Recherche Agronomique UM2 Unité Mixte de Recherche 5004, Place Viala, F 34060 Montpellier Cedex 1, France
    Plant Cell 15:509-22. 2003
    b>Aquaporins are ubiquitous channel proteins that facilitate the transport of water across cell membranes. Aquaporins show a typically high isoform multiplicity in plants, with 35 homologs in Arabidopsis...
  36. ncbi Aquaporins in drug discovery and pharmacotherapy
    Vincent J Huber
    Center for Integrated Human Brain Science, Brain Research Institute, University of Niigata, 1 Asahi Machi Dori, Chuo Ku, Niigata 951 8585, Japan
    Mol Aspects Med 33:691-703. 2012
    ..We believe these studies and compounds form the basis from which future treatments and diagnostic protocols of aquaporin-based disorders will be developed...
  37. pmc AKAP2 anchors PKA with aquaporin-0 to support ocular lens transparency
    Matthew G Gold
    Department of Pharmacology, Howard Hughes Medical Institute, University of Washington School of Medicine, Seattle, WA, USA
    EMBO Mol Med 4:15-26. 2012
    ..Thus, anchored PKA modulation of AQP0 is a homeostatic mechanism that must be physically intact to preserve lens transparency...
  38. pmc Cerulean cataract mapped to 12q13 and associated with a novel initiation codon mutation in MIP
    Xueshan Xiao
    State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat Sen University, Guangzhou, China
    Mol Vis 17:2049-55. 2011
    ..To identify the genetic defect in a large Chinese family with autosomal dominant cerulean cataract...
  39. pmc The role of aquaporins and membrane damage in chilling and hydrogen peroxide induced changes in the hydraulic conductance of maize roots
    Ricardo Aroca
    Division of Biological Sciences, University of California at San Diego, La Jolla, California, 92093 0116, USA
    Plant Physiol 137:341-53. 2005
    ..genotype is made possible by avoiding or repairing membrane damage and by a greater abundance and/or activity of aquaporins. The same changes in aquaporins take place in the chilling-sensitive genotype, but we postulate that membrane ..
  40. ncbi Aquaporin expression is downregulated in a murine model of colitis and in patients with ulcerative colitis, Crohn's disease and infectious colitis
    J A Hardin
    The Gastrointestinal Research Unit, University of Calgary, Calgary, AB, Canada
    Cell Tissue Res 318:313-23. 2004
    ..Recently, endogenous water channel proteins (aquaporins; AQPs), have been identified in colonic tissue...
  41. ncbi Aquaporin homologues in plants and mammals transport ammonia
    Thomas P Jahn
    Plant Nutrition Laboratory, Department of Agricultural Sciences, The Royal Veterinary and Agricultural University, Thorvaldsensvej 40, DK 1871 Frederiksberg C, Denmark
    FEBS Lett 574:31-6. 2004
    ..modeling of TaTIP2;1 in combination with site directed mutagenesis suggested a new subgroup of NH3-transporting aquaporins here called aquaammoniaporins...
  42. ncbi Mechanisms of water transport mediated by PIP aquaporins and their regulation via phosphorylation events under salinity stress in barley roots
    Tomoaki Horie
    Group of Molecular and Functional Plant Biology, Institute of Plant Science and Resources, Okayama University, 20 1 Chuo 2 chome, Kurashiki, Okayama 710 0046, Japan
    Plant Cell Physiol 52:663-75. 2011
    ..Here we report the water transport activity and mechanisms for the regulation of barley (Hordeum vulgare) PIP aquaporins. HvPIP2 but not HvPIP1 channels were found to show robust water transport activity when expressed alone in ..
  43. ncbi Cytosolic pH regulates root water transport during anoxic stress through gating of aquaporins
    Colette Tournaire-Roux
    Biochimie et Physiologie Moleculaire des Plantes, Centre National de la Recherche Scientifique Unité Mixte de Recherche 5004, Institut National de la Recherche Agronomique, Université Montpellier 2 et Ecole Nationale d Agronomie, Montpellier, France
    Nature 425:393-7. 2003
    ..Root water uptake is mediated largely by water channel proteins (aquaporins) of the plasma membrane intrinsic protein (PIP) subgroup...
  44. ncbi The role of aquaporin RWC3 in drought avoidance in rice
    Hong Li Lian
    Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 300 Fenglin Road, Shanghai, 200032, PR China
    Plant Cell Physiol 45:481-9. 2004
    Although the discovery of aquaporins in plants has resulted in a paradigm shift in the understanding of plant water relations, the relationship between aquaporins and drought resistance still remains elusive...
  45. ncbi Regulation of plant aquaporin activity
    François Chaumont
    Unite de Biochimie Physiologique, Institut des Science de la Vie, Universite Catholique de Louvain, Croix du Sud 2 20, B 1348 Louvain la Neuve, Belgium
    Biol Cell 97:749-64. 2005
    Accumulating evidence indicates that aquaporins play a key role in plant water relations...
  46. ncbi Functional characterization of drought-responsive aquaporins in Populus balsamifera and Populus simonii×balsamifera clones with different drought resistance strategies
    Adriana M Almeida-Rodriguez
    Department of Renewable Resources, University of Alberta, Edmonton, Alberta, Canada T6E 2E3
    Physiol Plant 140:321-33. 2010
    We have characterized poplar aquaporins (AQPs) to investigate their possible functions in differential drought responses of Populus balsamifera and Populus simonii×balsamifera leaves...
  47. pmc Cloning, functional characterization, and co-expression studies of a novel aquaporin (FaPIP2;1) of strawberry fruit
    Karina Alleva
    Laboratorio de Biomembranas, Departamento de Fisiologia, Facultad de Medicina, Universidad de Buenos Aires, Argentina
    J Exp Bot 61:3935-45. 2010
    In strawberry, the putative participation of aquaporins should be considered during fruit ripening...
  48. ncbi Erythrocyte water permeability and renal function in double knockout mice lacking aquaporin-1 and aquaporin-3
    B Yang
    Department of Medicine, Cardiovascular Research Institute, University of California, San Francisco, California, 94143 0521, USA
    J Biol Chem 276:624-8. 2001
    ..The renal function studies indicate independent roles of AQP1 and AQP3 in countercurrent exchange and collecting duct osmotic equilibration, respectively...
  49. pmc Aquaporin tetramer composition modifies the function of tobacco aquaporins
    Beate Otto
    Department of Applied Plant Sciences, Institute of Botany, Darmstadt University of Technology, D 64287 Darmstadt, Germany
    J Biol Chem 285:31253-60. 2010
    ..NtPIP2;1, which belongs to the PIP2 subfamily of plant aquaporins, behaved as a true aquaporin but lacked a CO(2)-related function...
  50. ncbi Marine fish egg hydration is aquaporin-mediated
    Mercedes Fabra
    Center of Aquaculture Institut de Recerca i Tecnologia Agroalimentaries, Tarragona, Spain, and Reference Center in Aquaculture, Barcelona, Spain
    Science 307:545. 2005
    ....
  51. ncbi Overexpression of the barley aquaporin HvPIP2;1 increases internal CO(2) conductance and CO(2) assimilation in the leaves of transgenic rice plants
    Yuko T Hanba
    Research Institute for Bioresources, Okayama University, Kurashiki, 710 0046 Japan
    Plant Cell Physiol 45:521-9. 2004
    ..Although such anatomical changes could partially offset the increase in g(i) by the aquaporin, the increase in aquaporin content overcame such adverse effects...
  52. ncbi Structure and function of aquaporin water channels
    A S Verkman
    Departments of Medicine and Physiology, Cardiovascular Research Institute, University of California, San Francisco 94143 0521, USA
    Am J Physiol Renal Physiol 278:F13-28. 2000
    The aquaporins (AQPs) are a family of small membrane-spanning proteins (monomer size approximately 30 kDa) that are expressed at plasma membranes in many cells types involved in fluid transport...
  53. ncbi Plant aquaporins: novel functions and regulation properties
    Christophe Maurel
    Biochimie et Physiologie Moleculaire des Plantes, Agro M CNRS INRA UM2 UMR 5004, Place Viala, F 34060 Montpellier Cedex 1, France
    FEBS Lett 581:2227-36. 2007
    b>Aquaporins are water channel proteins of intracellular and plasma membranes that play a crucial role in plant water relations...
  54. ncbi Severely impaired urinary concentrating ability in transgenic mice lacking aquaporin-1 water channels
    T Ma
    Department of Medicine, Cardiovascular Research Institute, University of California, San Francisco, California 94143 0521, USA
    J Biol Chem 273:4296-9. 1998
    ..in human subjects deficient in AQP1 (Colton null blood group) and in AQP4 knockout mice has suggested that aquaporins (other than the vasopressin-regulated water channel AQP2) may not be important in mammalian physiology...
  55. ncbi Aquaporin water channels in mammals
    Kenichi Ishibashi
    Department of Medical Physiology, Meiji Pharmaceutical University, 2 522 1 Noshio, Kiyose, Tokyo, 204 8588, Japan
    Clin Exp Nephrol 13:107-17. 2009
    Water channels, aquaporins (AQPs), are a family of small integral plasma membrane proteins that primarily transport water across the plasma membrane. There are 13 members (AQP0-12) in humans...
  56. ncbi Water permeation across biological membranes: mechanism and dynamics of aquaporin-1 and GlpF
    B L de Groot
    Theoretical Molecular Biophysics Group, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    Science 294:2353-7. 2001
    ..In AQP1, a fine-tuned water dipole rotation during passage is essential for water selectivity. In GlpF, a glycerol-mediated "induced fit" gating motion is proposed to generate selectivity for glycerol over water...
  57. pmc Expression and function of water channels (aquaporins) in migrating malignant astrocytes
    Eric McCoy
    Department of Neurobiology, Center for Glial Biology in Medicine, University of Alabama at Birmingham, Birmingham, Alabama, USA
    Glia 55:1034-43. 2007
    b>Aquaporins (AQP) constitute the principal pathway for water movement across biological membranes. Consequently, their expression and function is important for cell volume regulation...
  58. ncbi Identification and characterization of two plasma membrane aquaporins in durum wheat (Triticum turgidum L. subsp. durum) and their role in abiotic stress tolerance
    Malika Ayadi
    Plant Protection and Improvement Laboratory, Centre of Biotechnology of Sfax, University of Sfax, route Sidi mansour Km 6, BP 1177, 3018 Sfax, Tunisia
    Plant Physiol Biochem 49:1029-39. 2011
    ..its co-expression with the functional TdPIP2;1 partially inhibits the P(f) and disrupt the activities of plant aquaporins. In contrast to the approach developed in Xenopus oocytes, phenotypic analyses of transgenic tobacco plants ..
  59. ncbi Plant aquaporins with non-aqua functions: deciphering the signature sequences
    Runyararo Memory Hove
    Environment and Biotechnology Centre, Faculty of Life and Social Sciences, Swinburne University of Technology, P O Box 218, Hawthorn, Vic, 3122, Australia
    Plant Mol Biol 75:413-30. 2011
    Research in recent years on plant Major Intrinsic Proteins (MIPs), commonly referred to as 'aquaporins', has seen a vast expansion in the substrates found to be transported via these membrane channels...
  60. ncbi Regulation of aquaporin water permeability in the lens
    Kulandaiappan Varadaraj
    Department of Physiology and Biophysics, State University of New York at Stony Brook, Stony Brook, New York, USA
    Invest Ophthalmol Vis Sci 46:1393-402. 2005
    ..To examine Ca(2+)- and pH-mediated regulation of water permeability of endogenously expressed aquaporin (AQP)0 in lens fiber cells and AQP1 in lens epithelial cells...
  61. ncbi Developmental expression and biophysical characterization of a Drosophila melanogaster aquaporin
    Nancy Kaufmann
    Department of Biological Sciences, University of Pittsburgh, Pittsburgh, PA 15260, USA
    Am J Physiol Cell Physiol 289:C397-407. 2005
    b>Aquaporins (AQPs) accelerate the movement of water and other solutes across biological membranes, yet the molecular mechanisms of each AQP's transport function and the diverse physiological roles played by AQP family members are still ..
  62. pmc Identification of the family of aquaporin genes and their expression in upland cotton (Gossypium hirsutum L.)
    Wonkeun Park
    USDA ARS, Coastal Plains Soil, Water and Plant Research Center, Florence, SC 29501, USA
    BMC Plant Biol 10:142. 2010
    ..The plant aquaporins belong to the large major intrinsic protein (MIP) family...
  63. pmc Aquaporins and cell migration
    M C Papadopoulos
    Department of Medicine and Physiology, University of California, San Francisco, CA, USA
    Pflugers Arch 456:693-700. 2008
    ..AQP inhibitors may thus have therapeutic potential in modulating these events, such as slowing tumor growth and spread, and reducing glial scarring after injury to allow neuronal regeneration...
  64. pmc Genome-wide analysis of major intrinsic proteins in the tree plant Populus trichocarpa: characterization of XIP subfamily of aquaporins from evolutionary perspective
    Anjali Bansal Gupta
    Department of Biological Sciences and Bioengineering, Indian Institute of Technology Kanpur, Kanpur 208016, India
    BMC Plant Biol 9:134. 2009
    Members of major intrinsic proteins (MIPs) include water-conducting aquaporins and glycerol-transporting aquaglyceroporins. MIPs play important role in plant-water relations...
  65. ncbi Aquaporin water channels: molecular mechanisms for human diseases
    Peter Agre
    Departments of Biological Chemistry and Medicine, Johns Hopkins School of Medicine, Baltimore, MD 21205 2185, USA
    FEBS Lett 555:72-8. 2003
    ..Analysis of several human disease states has confirmed that aquaporins are involved in multiple different illnesses including abnormalities of kidney function, loss of vision, onset ..
  66. pmc A novel mutation in the major intrinsic protein (MIP) associated with autosomal dominant congenital cataracts in a Chinese family
    Wei Wang
    Eye Center of the 2nd Affiliated Hospital, Medical College of Zhejiang University, Hangzhou, China
    Mol Vis 16:534-9. 2010
    ..To detect the underlying genetic defect in a Chinese family affected with bilateral congenital cataracts...
  67. pmc Involvement of HbPIP2;1 and HbTIP1;1 aquaporins in ethylene stimulation of latex yield through regulation of water exchanges between inner liber and latex cells in Hevea brasiliensis
    Kessarin Tungngoen
    Department of Biotechnology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand
    Plant Physiol 151:843-56. 2009
    ....
  68. ncbi Function of a separate NH3-pore in Aquaporin TIP2;2 from wheat
    Adam Bertl
    Institute of Botany, Applied Plant Science, Darmstadt University of Technology, Schnittspahnstrasse 10, D 64287 Darmstadt, Germany
    FEBS Lett 581:5413-7. 2007
    ..Results from inhibitor studies strongly suggest that NH(3) is not transported in file with water, but through a separate pathway, which could be supplied by the 5th central pore in a tetramer conformation...
  69. ncbi As(III) and Sb(III) uptake by GlpF and efflux by ArsB in Escherichia coli
    Yu Ling Meng
    Department of Biochemistry and Molecular Biology, Wayne State University, School of Medicine, Detroit, Michigan 48201, USA
    J Biol Chem 279:18334-41. 2004
    ..We propose that the actual substrate of ArsB is a polymer of (AsO)(n), (SbO)(n), or a co-polymer of the two metalloids...
  70. pmc MIP/Aquaporin 0 represents a direct transcriptional target of PITX3 in the developing lens
    Elena A Sorokina
    Department of Pediatrics and Children s Research Institute, Medical College of Wisconsin and Children s Hospital of Wisconsin, Milwaukee, Wisconsin, United States of America
    PLoS ONE 6:e21122. 2011
    ..Therefore, our data suggest that PITX3 is involved in direct regulation of MIP/AQP0 expression and that the alteration of MIP/AQP0 expression is likely to contribute to the lens phenotype in cataract patients with PITX3 mutations...
  71. pmc The aromatic/arginine selectivity filter of NIP aquaporins plays a critical role in substrate selectivity for silicon, boron, and arsenic
    Namiki Mitani-Ueno
    Institute of Plant Science and Resources, Okayama University, Chuo 2 20 1, Kurashiki 710 0046, Japan
    J Exp Bot 62:4391-8. 2011
    ..investigate how the aromatic/arginine (ar/R) selectivity filter influences the substrate selectivity of two NIP aquaporins; the silicic acid (Si) transporter OsLsi1 (OsNIP2;1) from rice and the boric acid (B) transporter AtNIP5;1 from ..
  72. ncbi Aquaporins and plant water balance
    Ralf Kaldenhoff
    Technische Universitat Darmstadt, Institute of Botany, Applied Plant Science, Schnittspahnstr 10, D 64287 Darmstadt, Germany
    Plant Cell Environ 31:658-66. 2008
    ..for root water uptake, water conductance in the xylem, including embolism refilling and the role of plant aquaporins in leaf physiology, is described...
  73. pmc Aquaporins--new players in cancer biology
    A S Verkman
    Department of Medicine, University of California, 1246 Health Sciences East Tower, San Francisco, CA 94143 0521, USA
    J Mol Med (Berl) 86:523-9. 2008
    The aquaporins (AQPs) are small, integral-membrane proteins that selectively transport water across cell plasma membranes. A subset of AQPs, the aquaglyceroporins, also transport glycerol...
  74. ncbi Enhancement of the aquaporin adipose gene expression by a peroxisome proliferator-activated receptor gamma
    K Kishida
    Department of Internal Medicine and Molecular Science, Graduate School of Medicine, Osaka University, 2 2 Yamadaoka, Suita 565 0871, Japan
    J Biol Chem 276:48572-9. 2001
    ..We conclude that AQPap is a novel adipose-specific target gene of PPARgamma through the binding of PPARgamma-retinoid X receptor complex to the PPRE region in its promoter...
  75. ncbi Progressive adipocyte hypertrophy in aquaporin-7-deficient mice: adipocyte glycerol permeability as a novel regulator of fat accumulation
    Mariko Hara-Chikuma
    Department of Medicine, Cardiovascular Research Institute, University of California, San Francisco, California 94143, USA
    J Biol Chem 280:15493-6. 2005
    ..Increasing AQP7 expression/function in adipocytes may reduce adipocyte volume and fat mass in obesity...
  76. ncbi Aquaporins: another piece in the osmotic puzzle
    Karina Alleva
    Departamento de Biodiversidad y Biologia Experimental, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires and Consejo Nacional de Investigaciones Científicas y Técnicas CONICET, Buenos Aires, Argentina
    FEBS Lett 586:2991-9. 2012
    ..The molecular understanding of osmosis has not yet been completely achieved, and the discovery of aquaporins as molecular entities involved in water transport has caused osmosis to again become a focus of research...
  77. pmc Antimonite is accumulated by the glycerol facilitator GlpF in Escherichia coli
    O I Sanders
    Department of Biochemistry and Molecular Biology, Wayne State University School of Medicine, Detroit, Michigan 48201, USA
    J Bacteriol 179:3365-7. 1997
    ..The mutant was shown to be defective in polyol transport by GlpF. These results suggest that in solution Sb(III) is recognized as a polyol by the glycerol facilitator...
  78. pmc Aquaporin-9 protein is the primary route of hepatocyte glycerol uptake for glycerol gluconeogenesis in mice
    Sabina Jelen
    From the Water and Salt Research Center, Department of Biomedicine, Aarhus University, Wilhelm Meyers Alle, DK 8000 Aarhus C, Denmark
    J Biol Chem 286:44319-25. 2011
    ..Our experiments establish AQP9 as the primary route of hepatocyte glycerol uptake for gluconeogenesis and thereby explain the previously observed, alleviated diabetes in leptin receptor-deficient AQP9(-/-) mice...
  79. ncbi Cyclic AMP regulates aquaporin 5 expression at both transcriptional and post-transcriptional levels through a protein kinase A pathway
    Fan Yang
    University of Cincinnati College of Medicine, Department of Molecular Genetics, Biochemistry and Microbiology, Cincinnati, Ohio 45267, USA
    J Biol Chem 278:32173-80. 2003
    ..These results show that cAMP regulates AQP5 at multiple levels, by increasing synthesis of AQP5 mRNA and by triggering translocation of AQP5 to the plasma membrane...
  80. pmc Developmental pattern of aquaporin expression in barley (Hordeum vulgare L.) leaves
    Matthieu Besse
    UCD School of Biology and Environmental Sciences, Science Centre West, University College Dublin, Belfield, Dublin 4, Ireland
    J Exp Bot 62:4127-42. 2011
    b>Aquaporins are multifunctional membrane channels which belong to the family of major intrinsic proteins (MIPs) and are best known for their ability to facilitate the movement of water...
  81. ncbi TIP5;1 is an aquaporin specifically targeted to pollen mitochondria and is probably involved in nitrogen remobilization in Arabidopsis thaliana
    Gabriela Soto
    Instituto de Ingeniería Genética y Biología Molecular INGEBI CONICET, Vuelta de Obligado 2490 Piso 2, 1428 Buenos Aires, Argentina
    Plant J 64:1038-47. 2010
    In plant sexual reproduction, water and solute movement are tightly regulated, suggesting the involvement of aquaporins. We previously identified TIP5;1 and TIP1;3 as the only Arabidopsis aquaporin genes that are selectively and highly ..
  82. ncbi Regulation of collecting duct AQP3 expression: response to mineralocorticoid
    Tae Hwan Kwon
    The Water and Salt Research Center, University of Aarhus, DK 8000 Aarhus C, Denmark
    Am J Physiol Renal Physiol 283:F1403-21. 2002
    ..Therefore, in the collecting duct aldosterone may regulate, at least in part, AQP3 expression in addition to regulating Na and K transport...
  83. pmc Transgenic expression of AQP1 in the fiber cells of AQP0 knockout mouse: effects on lens transparency
    K Varadaraj
    Department of Physiology and Biophysics, State University of New York at Stony Brook, NY 11794 8661, USA
    Exp Eye Res 91:393-404. 2010
    ..To our knowledge, this is the first report which uses an animal model to demonstrate that AQP0 may have an additional function, possibly cell-to-cell adhesion...
  84. ncbi Influence of nitrogen fertilization on xylem traits and aquaporin expression in stems of hybrid poplar
    Uwe G Hacke
    Department of Renewable Resources, University of Alberta, Edmonton, AB, T6G 2E3, Canada
    Tree Physiol 30:1016-25. 2010
    ..We suggest that some of these water-transporting AQPs could play a role in xylogenesis, facilitating the influx of water into the zone of differentiating and maturing cells in secondary xylem, including expanding vessels...
  85. ncbi Solanaceae XIPs are plasma membrane aquaporins that facilitate the transport of many uncharged substrates
    Gerd Patrick Bienert
    Institut des Sciences de la Vie, Universite Catholique de Louvain, Croix du Sud 4 15, B 1348 Louvain la Neuve, Belgium
    Plant J 66:306-17. 2011
    ..These data suggest that XIPs function in the transport of uncharged solutes across the cell plasma membrane in specific plant tissues, including at the interface between the environment and external cell layers...
  86. ncbi Molecular basis of proton blockage in aquaporins
    Nilmadhab Chakrabarti
    Structural Biology and Biochemistry, Hospital for Sick Children, 555 University Avenue, Toronto, Ontario M5G 1X8, Canada
    Structure 12:65-74. 2004
    ..Inversely, translocation of an excess proton in either direction is opposed by a free-energy barrier centered at the NPA region. Both hop and turn steps of proton translocation are opposed by the electrostatic field of the channel...
  87. pmc Structural analysis of cloned plasma membrane proteins by freeze-fracture electron microscopy
    S Eskandari
    Department of Physiology, University of California at Los Angeles School of Medicine, Los Angeles, CA 90095 1751, USA
    Proc Natl Acad Sci U S A 95:11235-40. 1998
    ....
  88. pmc Function of Nicotiana tabacum aquaporins as chloroplast gas pores challenges the concept of membrane CO2 permeability
    Norbert Uehlein
    Department of Applied Plant Sciences, Institute of Botany, Darmstadt University of Technology, D 64287 Darmstadt, Germany
    Plant Cell 20:648-57. 2008
    ..Our discovery of CO(2) aquaporin function in the chloroplast membrane opens new opportunities for mechanistic examination of leaf internal CO(2) conductance regulation...
  89. pmc Mechanism of selectivity in aquaporins and aquaglyceroporins
    Jochen S Hub
    Computational Biomolecular Dynamics Group, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    Proc Natl Acad Sci U S A 105:1198-203. 2008
    b>Aquaporins and aquaglyceroporins form a family of pore proteins that facilitate the efficient and selective flux of small solutes across biological membranes...
  90. ncbi Expression and localisation of aquaporin water channels in human urothelium in situ and in vitro
    Peter C Rubenwolf
    Jack Birch Unit for Molecular Carcinogenesis, Department of Biology, University of York, York, UK
    Eur Urol 56:1013-23. 2009
    ..The possibility that human urothelium expresses aquaporin (AQP) water channels as the basis for water and solute transport has not previously been investigated...
  91. pmc Long-range nonanomalous diffusion of quantum dot-labeled aquaporin-1 water channels in the cell plasma membrane
    Jonathan M Crane
    Department of Medicine, Cardiovascular Research Institute, University of California, San Francisco, California 94143 0521, USA
    Biophys J 94:702-13. 2008
    ....
  92. pmc Incipient balancing selection through adaptive loss of aquaporins in natural Saccharomyces cerevisiae populations
    Jessica L Will
    Laboratory of Genetics, University of Wisconsin Madison, Madison, Wisconsin, United States of America
    PLoS Genet 6:e1000893. 2010
    ..This is consistent with recent adaptive loss of aquaporins in subgroups of strains, leading to incipient balancing selection...
  93. ncbi Purification and functional characterization of aquaporin-8
    Kun Liu
    Department of Biological Chemistry, School of Medicine, Johns Hopkins University, Baltimore, MD 21205, USA
    Biol Cell 98:153-61. 2006
    b>Aquaporins (AQPs) are a family of channels permeable to water and some small solutes. In mammals, 13 members (AQP0-AQP12) have been found. AQP8 is widely distributed in many tissues and organs...
  94. pmc The NPC motif of aquaporin-11, unlike the NPA motif of known aquaporins, is essential for full expression of molecular function
    Masahiro Ikeda
    Department of Veterinary Pharmacology, University of Miyazaki, Miyazaki 889 2192, Japan
    J Biol Chem 286:3342-50. 2011
    ..Although the NPA motif is known to be important for formation of water-permeable pores by conventional AQPs, our observations suggest that the corresponding NPC motif of AQP11 is essential for full expression of molecular function...
  95. pmc A novel mutation in MIP associated with congenital nuclear cataract in a Chinese family
    Kai jie Wang
    Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing Ophthalmology and Visual Sciences Key Lab, Beijing, China
    Mol Vis 17:70-7. 2011
    ..To identify the underlying genetic defect in a Chinese family affected with autosomal dominant congenital nuclear cataract...
  96. ncbi Water channel proteins (later called aquaporins) and relatives: past, present, and future
    Gheorghe Benga
    Department of Cell and Molecular Biology, Iuliu Hatieganu University of Medicine and Pharmacy Cluj Napoca, Cluj Napoca, Romania
    IUBMB Life 61:112-33. 2009
    ..WCPs include three subfamilies: (a) aquaporins (AQPs), which are water specific (or selective water channels); (b) aquaglyceroporins (and glycerol facilitators)..
  97. ncbi Nickel and extracellular acidification inhibit the water permeability of human aquaporin-3 in lung epithelial cells
    Marina Zelenina
    Department of Woman and Child Health, Karolinska Institutet, S 171 76 Stockholm, Sweden
    J Biol Chem 278:30037-43. 2003
    ..Our results imply that nickel and extracellular pH may modulate lung water clearance and that defective water clearance may be an early component of nickel-induced lung disease...
  98. pmc Nephrogenic diabetes insipidus in mice lacking aquaporin-3 water channels
    T Ma
    Departments of Medicine, Physiology, and Pediatrics, Cardiovascular Research Institute, University of California, San Francisco, CA 94143 0521, USA
    Proc Natl Acad Sci U S A 97:4386-91. 2000
    ..Basolateral membrane aquaporins may thus provide blood-accessible targets for drug discovery of aquaretic inhibitors.
  99. ncbi Aquaporins in kidney pathophysiology
    Yumi Noda
    Department of Nephrology, Tokyo Medical and Dental University, Bunkyo ku, Tokyo 113 8519, Japan
    Nat Rev Nephrol 6:168-78. 2010
    ..A lot of data have increased understanding of the functions and mechanisms of regulation of aquaporins both at the molecular and the clinical level. Research has also focused on aquaporins as therapeutic targets...
  100. ncbi Arsenic uptake and metabolism in plants
    F J Zhao
    Soil Science Department, Rothamsted Research, Harpenden, Hertfordshire AL5 2JQ, UK
    New Phytol 181:777-94. 2009
    ..Current knowledge gaps and future research directions are also identified...
  101. ncbi Copper inhibits the water and glycerol permeability of aquaporin-3
    Marina Zelenina
    Department of Woman and Child Health, Karolinska Institutet, Stockholm S 171 77, Sweden
    J Biol Chem 279:51939-43. 2004
    ..Our results provide a better understanding of processes that occur in severe copper metabolism defects such as Wilson disease and in copper poisoning...

Research Grants63

  1. The role of polyphosphate and acidocalcisomes in Trypanosoma brucei
    Roberto Docampo; Fiscal Year: 2013
    ..contains a number of pumps (Ca2+- ATPase, V-H+-ATPase, H+-PPase), exchangers (Na+/H+, Ca2+/H+), and channels (aquaporins), while its matrix contains enzymes related to pyrophosphate and polyphosphate metabolism...
  2. Therapeutic Intervention of Lewisite-Mediated Cutaneous Blistering-Inflammation
    Mohammad Athar; Fiscal Year: 2013
    ..in epidermis. In addition, proteins involved in the regulation of water/glycerin transport, the aquaporins, will be studied...
  3. Using zebrafish to define a novel role for AQP2 in kidney tubulogenesis
    HUA A LU; Fiscal Year: 2010
    ..Water channels, also named aquaporins, are present in many cell types and tissues, plants and lower organisms. AQP2 is one of this family...
  4. Regulation of Urea Transport in Diabetic Rat Kidney
    Janet Klein; Fiscal Year: 2009
    ..Specific Aim 1: we will test whether expression of urea transporters, aquaporins, and ion channels or transporters are altered in rats with uncontrolled diabetes...
  5. BIOMECHANICS OF BRAIN EDEMA AND INTRACRANIAL PRESSURE
    CHRISTINA ROSE MARMAROU; Fiscal Year: 2012
    ..To this purpose we base our future work on three hypotheses encompassing (1) clarifying the role of aquaporins in traumatic brain injury by identifying the pathway of sodium and water entry into brain after experimental ..
  6. Aquaporin-4 in NMO Optic Neuritis: Pathogenesis and New Therapies
    Alan S Verkman; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Aquaporins (AQPs) are water transporting proteins expressed widely in mammalian tissues including the eye...
  7. Human Amnion Cell Model for Investigation of Amniotic Fluid Volume
    Cecilia Y Cheung; Fiscal Year: 2012
    ..In Specific Aim 2, the VEGF165 regulation of passive diffusion across water channels aquaporins 1, 3 and 9 in human amnion cells will be examined...
  8. Synaptic Circuitry in Stroke
    Sergei A Kirov; Fiscal Year: 2013
    ..SD-induced cytotoxic edema contributes to stroke injury. Mammalian pyramidal neurons lack functional aquaporins, thus the molecular pathways by which they accumulate osmotically obligated water and rapidly swell during SD is ..
  9. AQP4 and JNK Inhibition Together Reduce Edema and Excitotoxic Injury in jTBI
    Jerome Badaut; Fiscal Year: 2013
    ..Recent studies have demonstrated that two novel proteins/pathways (aquaporins, AQPs;c-Jun N terminal kinase, c-JNK) play critical roles at different overlapping points in the cascade of ..
  10. Renal and Electrolyte Disease and Hypertention
    Tomas Berl; Fiscal Year: 2012
    ..include: a) laboratories studying the pathogenesis of acute renal injury;b) laboratories exploring the role of aquaporins in disorders of water balance;c) laboratories focused on singaling pathways in vascular smooth muscle cells, ..
  11. Origins of Renal Physiology
    Mark L Zeidel; Fiscal Year: 2011
    ..cellular trafficking of transporter proteins, and the structure-function of transporter proteins such as aquaporins, urea carriers, ENaC and the Na/K/2Cl cotransporter...
  12. Genetic information transfer to hematopoietic cells: Role of microvesicles
    Peter J Quesenberry; Fiscal Year: 2013
    ..and effect a change in genetic phenotype manifest by expression of the lung-specific mRNAs;surfactants A-D, aquaporins and clara cell specific protein and in function manifest by increased conversion to epithelial lung cells after ..
  13. Theoretical study of structure-function correlations of Aquaporin V and mutants
    LIAO Y CHEN; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Among the thirteen human aquaporins (AQP0-AQP12) distributed within cells of the stomach, duodenum, pancreas, airways, lungs, salivary glands, sweat glands, eyes, lacrimal glands, and the inner ear, ..
  14. Mechanisms of Polyptopic Protein Biogenesis in the ER
    William R Skach; Fiscal Year: 2013
    ..b>Aquaporins comprise a conserved family of 6-spanning, homotetrameric membrane proteins that play critical roles in water ..
  15. Origins of Renal Physiology
    Mark L Zeidel; Fiscal Year: 2010
    ..ascending limb, and collecting duct transport, water homeostasis, cellular trafficking of transporter proteins, and the structure-function of transporter proteins such as aquaporins, urea carriers, ENaC and the Na/K/2Cl co-transporter.
  16. Role of Aquaporin-0 for cell-to-cell adhesion and lens transparency
    Kulandaiappan Varadaraj; Fiscal Year: 2013
    ..Transmembrane water channels known as aquaporins (AQPs) play a significant role in maintaining transparency and homeostasis in the avascular lens...
  17. MECHANISMS OF WATER TRANSPORT IN RENAL EPITHELIA
    Alan S Verkman; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Aquaporins (AQPs) are membrane water channels expressed in many mammalian tissues that carry out fluid transport...
  18. Fetal Membrane AQP Expression/Amniotic Fluid Regulation
    Michael Ross; Fiscal Year: 2004
    ..b>Aquaporins (AQPs) are cell membrane water channel proteins that greatly enhance cell membrane water permeability...
  19. BIOMECHANICS OF BRAIN EDEMA AND INTRACRANIAL PRESSURE
    Anthony Marmarou; Fiscal Year: 2007
    ..To this purpose we base our future work on three hypotheses encompassing (1) clarifying the role of Aquaporins in traumatic brain injury by identifying the pathway of sodium and water entry into brain after experimental ..
  20. REGULATION OF THE RENAL SALT AND WATER TRANSPORTERS
    Carolyn Ecelbarger; Fiscal Year: 2001
    ..on understanding those proteins which are important in the urinary concentrating mechanism, including the renal aquaporins and the Na- K-2Cl cotransporter. A key project of Dr. Ecelbarger's, done in collaboration with Dr...
  21. Structure and Function of Ocular Lacritin
    GORDON LAURIE; Fiscal Year: 2007
    ..also affecting aquaporins); and does bacterial infection increase tear lacritin and release lacritin's bacteriocidal fragment?
  22. MECHANISMS OF WATER FLOW ACROSS BIOLOGICAL MEMBRANES
    Mark Zeidel; Fiscal Year: 2009
    ..permeability (P) range, from apical membranes of low P barrier epithelia to high P membranes which contain aquaporins (AQPs) or urea transporters UT's). This grant addresses two fundamental physiological questions: Aim #1...
  23. MOLECULAR PHYSIOLOGY AND STRUCTURE OF AQUAPORINS
    Alfred Van Hoek; Fiscal Year: 2002
    In mammalian tissues water transport is of critical importance for osmotic balance and is facilitated by aquaporins (AQPs), which are integral membrane proteins forming aqueous pores...
  24. WATER TRAFFICKING IN THE OVARY
    FRANCIS HUGHES; Fiscal Year: 2002
    ..Recently, it was discovered that a family of proteinaceous channels termed aquaporins facilitates transcellular transport of water in a number of tissues, including the testis...
  25. RED CELL AQUAPORIN-1 WATER TRANSPORT PROTEIN
    Peter Agre; Fiscal Year: 2002
    ..While multiple homologous aquaporins are now becoming recognized in other tissues, physical studies of human red cell AQP1 are revealing its ..
  26. Aquaporins in Drosophila melanogaster
    Nancy Kaufmann; Fiscal Year: 2005
    ..application are to: 1) Use mRNA in situ and immunohistochemistry to uncover the sites of action of putative aquaporins. 2) Determine the substrate specificity and pore permeability of putative Drosophila aquaporins...
  27. Salt and Water Transporter Expression in Aging Kidneys
    JAMES TERRIS; Fiscal Year: 2002
    ..Four water channels (aquaporins; AQP-1 to 4), four urea transporter isoforms (UT-A1 to 4), and several major sodium transporter proteins have ..
  28. Structure Analysis of Aquaporin Membrane Channels
    Mark Yeager; Fiscal Year: 2006
    The MIP family of water-selective transmembrane pore proteins, commonly known as "aquaporins", are responsible for the rapid movement of water across cell membranes, and are thus crucial for solute transport and cellular, tissue, and ..
  29. SECOND INTERNATIONAL NOMENCLATURE WORKSHOP
    Muriel Davisson; Fiscal Year: 1999
    ..representing various species (vertebrates, invertebrates, yeast, plants), gene families (calcium channels, aquaporins, carbonic anhydrases, annexins), and databases (MGI, GDB, NCBI, SWISS-PROT) will discuss nomenclature issues ..
  30. Aquaporins and Neuropathic Pain after Spinal Cord Injury
    Olivera Nesic Taylor; Fiscal Year: 2009
    ..Our hypothesis on the involvement of AQP4, AQP1 and impaired osmoregulation in the pathogenesis of neuropathic pain conditions after SCI is novel and has not been previously studied. Project Description Page 6 ..
  31. Generation of Collecting Duct Knockout of PPARgamma
    Tianxin Yang; Fiscal Year: 2006
    ..Furthermore, PPARgamma activators stimulate renal expressions of various transporters such as aquaporins 2 and 3, NKCC2, alpha-1 subunit of Na-K ATPase in vivo and enhance cell surface ENaCa in human collecting duct (..
  32. WATER MOVEMENT DURING CELL DEATH
    FRANCIS HUGHES; Fiscal Year: 2002
    ..and hypothesize that water movement during apoptosis is mediated by preliminary data that general inhibitors of aquaporins block granulosa cell shrinkage during apoptosis...
  33. Origins of Renal Physiology
    Mark Zeidel; Fiscal Year: 2009
    ..ascending limb, and collecting duct transport, water homeostasis, cellular trafficking of transporter proteins, and the structure-function of transporter proteins such as aquaporins, urea carriers, ENaC and the Na/K/2Cl cotransporter.
  34. Role of Aquaporin in vocal fold epithelial water fluxes.
    Danielle Lodewyck; Fiscal Year: 2004
    ..If so, aquaporins may provide an intrinsic mechanism for regulation of vocal fold hydration and phonation...
  35. ROLE OF AQUAPORIN (AQP) 5 IN SALIVARY SECRETION
    Anil Menon; Fiscal Year: 2004
    ..suggests that water is transported in salivary acinar cells via a transcellular route through water channels, or aquaporins (AQPs). At least four AQPs are expressed in salivary glands...
  36. cCAMP Regulation of Aquaporin 5
    Venkataramana Sidhaye; Fiscal Year: 2004
    b>Aquaporins (AQPs) are water specific membrane channel proteins. AQP5, located in the apical membrane of airway submucosal glands, bronchial epithelium, and type I pneumocytes, likely participates in generation of airway surface secretions...
  37. Aquarporin Water Channel Proteins in Eye
    Peter Agre; Fiscal Year: 2005
    ..Four of the ten known mammalian aquaporins are expressed in anterior segment of eye...
  38. PRODUCE/TEST ADENOVIRUS VECTORS FOR CLINICAL USE
    JEFFREY OSTROVE; Fiscal Year: 2000
    ..Candidate proteins for this function have been identified (aquaporins), and preclinical data suggest that the aquaporins may be useful in improving the function of radiated salivary ..
  39. Modeling of Ion Pores
    Peter Jordan; Fiscal Year: 2007
    ..It is applied to aquaporins to better characterize the mechanism of proton rejection, and to elucidate the physical basis for high water ..
  40. STRUCTURE/FUNCTION OF MEMBRANE PROTEIN CHANNELS
    ALOK MITRA; Fiscal Year: 2002
    ..and identify at the structural level factors responsible for diversity in solute transport mediated by aquaporins. AIM IV. Structural studies on anthrax toxin protective antigen...
  41. Role of airway submucosal glands in CF lung disease
    Alan S Verkman; Fiscal Year: 2010
    ..The data will provide a rigorous scientific rationale for use of small-molecules in CF therapy, and establish widely useful assays to prioritize small molecules for clinical development. ..
  42. APPLICATION OF NOVEL OPTICAL METHODS TO CELL DYNAMICS
    Alan Verkman; Fiscal Year: 2001
    ..These studies will address the hypothesis that the membrane skeleton of these cells is a mobile, dynamic structure. ..
  43. APPLICATION OF NOVEL OPTICAL METHODS TO CELL DYNAMICS
    Alan Verkman; Fiscal Year: 2006
    ..abstract_text> ..
  44. PHYSIOLOGY AND MOLECULAR BIOLOGY OF LUNG WATER TRANSPORT
    Alan Verkman; Fiscal Year: 2007
    ..b>Aquaporins (AQPs) are water-transporting proteins expressed in lung microvasculature (AQP1), airways (AQP3 and AQP4), ..
  45. MECHANISMS OF WATER TRANSPORT IN RENAL EPITHELIA
    Alan Verkman; Fiscal Year: 2008
    ..The kidney expresses at least four aquaporins: AQP1 in proximal tubule, TDLH and vasa recta; AQP2 in apical membrane of principal cells in collecting duct; ..
  46. Molecular tracking of quantum dot-linked aquaporins in living cell membranes
    Jonathan Crane; Fiscal Year: 2008
    ..provided by applicant): The broad goal of the proposed research is to understand the molecular arrangements of aquaporins in a living cell membrane...
  47. ALTERED KCa CHANNEL EXPRESSION IN DEVELOPING CEREBELLUM
    ANDREA YOOL; Fiscal Year: 2002
    ..The rat provides a useful model for studying postnatal cerebellar development and neuropathological damage, and may provide insights into methods to manipulate developmental abnormalities in the cerebellum. ..
  48. TRANSPORT MECHANISMS IN CORNEAL ENDOTHELIUM
    Jorge Fischbarg; Fiscal Year: 2004
    ..The understanding to be gained may provide insights on how to modulate fluid transport so as to offset endothelial dysfunction leading to the loss of normal vision. ..
  49. Regulation of Urea Transport by Adrenal Steroids
    JEFF SANDS; Fiscal Year: 2005
    ..We also found that UT-A1 protein abundance is decreased during in vivo conditions associated with increased glucocorticoids. ..
  50. Aquaporin 5 (AQP5) in Lung Fluid Homeostasis
    CARISSA KRANE; Fiscal Year: 2004
    ..Control of membrane water flux through membrane water pores (aquaporins; AQP) is essential for the ability of an organism to adapt to changing fluid environments...
  51. SELENIUM-INDUCED GLAUCOMA
    W Stamer; Fiscal Year: 2006
    ..abstract_text> ..
  52. HYPONATREMIA-INDUCED OSTEOPOROSIS
    Joseph G Verbalis; Fiscal Year: 2010
    ....
  53. MICROVASCULAR TRANSPORT IN THE RENAL MEDULLA
    THOMAS PALLONE; Fiscal Year: 2002
    ..Exchange of albumin and middle molecular weight solutes will also be simulated. ..
  54. Fluid Movement across Conjunctiva: Stimulation and Regulation
    OSCAR CANDIA; Fiscal Year: 2007
    ..to identify with biochemical and histochemical protocols, the electrolyte transporters and water channels (aquaporins) in the human conjunctiva relevant towards the movement of fluid flow across the epithelium...
  55. Molecular Basis of Myocilin Function in the Human Eye
    W Daniel Stamer; Fiscal Year: 2010
    ..abstract_text> ..
  56. TECNIA 12 BioTWIN Transmission Electron Microscope
    Guido Zampighi; Fiscal Year: 2003
    ..abstract_text> ..
  57. VASOMOTOR CONTROL OF DESCENDING VASA RECTA
    THOMAS PALLONE; Fiscal Year: 2003
    ....
  58. SOLUTE AND FLUID TRANSPORT ACROSS THE CONJUNCTIVA
    OSCAR CANDIA; Fiscal Year: 2002
    ..Based on this model, he will devise protocols to modify and possibly reverse the secretory/absorptive properties of the tissue. ..
  59. GEORGETOWN UNIVERSITY-MEDSTAR CENTER FOR CLINICAL AND TRANSLATIONAL SCIENCE
    Joseph Verbalis; Fiscal Year: 2006
    ..The planning process will be led by a committee of faculty members who are leaders in translational research and clinical research training at the member institutions. [unreadable] [unreadable] [unreadable]..
  60. Calcium Signaling in Vasa Recta Endothelium
    THOMAS PALLONE; Fiscal Year: 2004
    ..We will test whether DVR contractions to norepinephrine, Angll and KCI are increased, measure NO production and assess effects of OH on endothelial and pericyte Ca2+ signaling. ..
  61. Reactive Oxygen Species American Society of Nephrology Fall 2007
    THOMAS PALLONE; Fiscal Year: 2007
    ..Attendance of young scientists insures that the information will be translated into future studies that elucidate the role of ROS in kidney diseases and treatment thereof. [unreadable] [unreadable] [unreadable]..
  62. Tools for Membrane Protein Structure Determination
    Vinzenz Unger; Fiscal Year: 2004
    ..This approach may make a large number of membrane proteins amenable to 2D-crystallization and structure determination. ..