porins

Summary

Summary: Porins are protein molecules that were originally found in the outer membrane of GRAM-NEGATIVE BACTERIA and that form multi-meric channels for the passive DIFFUSION of WATER; IONS; or other small molecules. Porins are present in bacterial CELL WALLS, as well as in plant, fungal, mammalian and other vertebrate CELL MEMBRANES and MITOCHONDRIAL MEMBRANES.

Top Publications

  1. pmc Molecular basis of bacterial outer membrane permeability revisited
    Hiroshi Nikaido
    Department of Molecular and Cell Biology, University of California, Berkeley, California 94720 3202, USA
    Microbiol Mol Biol Rev 67:593-656. 2003
  2. ncbi Bcl-2 family proteins regulate the release of apoptogenic cytochrome c by the mitochondrial channel VDAC
    S Shimizu
    Osaka University Medical School, Biomedical Research Center, Department of Medical Genetics, Suita, Japan
    Nature 399:483-7. 1999
  3. pmc Adaptive and mutational resistance: role of porins and efflux pumps in drug resistance
    Lucía Fernández
    Centre for Microbial Diseases and Immunity Research, Department of Microbiology and Immunology, University of British Columbia, Vancouver, British Columbia, Canada
    Clin Microbiol Rev 25:661-81. 2012
  4. ncbi Coding sequence targeting by MicC RNA reveals bacterial mRNA silencing downstream of translational initiation
    Verena Pfeiffer
    Max Planck Institute for Infection Biology, RNA Biology Group, Berlin, Germany
    Nat Struct Mol Biol 16:840-6. 2009
  5. pmc Outer membrane permeability and antibiotic resistance
    Anne H Delcour
    Department of Biology, University of Houston, 369 Science and Research Building II, Houston, TX 77204 5001, USA
    Biochim Biophys Acta 1794:808-16. 2009
  6. ncbi A small non-coding RNA of the invasion gene island (SPI-1) represses outer membrane protein synthesis from the Salmonella core genome
    Verena Pfeiffer
    Max Planck Institute for Infection Biology, Chariteplatz 1, D 10117 Berlin, Germany
    Mol Microbiol 66:1174-91. 2007
  7. pmc Klebsiella pneumoniae outer membrane porins OmpK35 and OmpK36 play roles in both antimicrobial resistance and virulence
    Yu Kuo Tsai
    Division of Infectious Diseases, National Health Research Institutes, Miaoli, Taiwan
    Antimicrob Agents Chemother 55:1485-93. 2011
  8. ncbi Porins, efflux pumps and multidrug resistance in Acinetobacter baumannii
    Jordi Vila
    Servei de Microbiologia, Centre de Diagnostic Biomedic, Hospital Clinic, IDIBAPS, Facultat de Medicina, Universitat de Barcelona, Barcelona, Spain
    J Antimicrob Chemother 59:1210-5. 2007
  9. ncbi The porin and the permeating antibiotic: a selective diffusion barrier in Gram-negative bacteria
    Jean Marie Pages
    UMR MD 1, Transporteurs Membranaires, Chimioresistance et Drug Design, Facultés de Médecine et de harmacie, Universite de la Mediterranee, Marseille, 13385, France
    Nat Rev Microbiol 6:893-903. 2008
  10. ncbi Sus1, a functional component of the SAGA histone acetylase complex and the nuclear pore-associated mRNA export machinery
    Susana Rodriguez-Navarro
    Biochemie Zentrum der Universität Heidelberg BZH, Im Neuenheimer Feld 328, D 69120 Heidelberg, Germany
    Cell 116:75-86. 2004

Detail Information

Publications315 found, 100 shown here

  1. pmc Molecular basis of bacterial outer membrane permeability revisited
    Hiroshi Nikaido
    Department of Molecular and Cell Biology, University of California, Berkeley, California 94720 3202, USA
    Microbiol Mol Biol Rev 67:593-656. 2003
    ..With the discovery of protein channels, structural knowledge enables us to understand in molecular detail how porins, specific channels, TonB-linked receptors, and other proteins function...
  2. ncbi Bcl-2 family proteins regulate the release of apoptogenic cytochrome c by the mitochondrial channel VDAC
    S Shimizu
    Osaka University Medical School, Biomedical Research Center, Department of Medical Genetics, Suita, Japan
    Nature 399:483-7. 1999
    ..Our results indicate that the Bcl-2 family of proteins bind to the VDAC in order to regulate the mitochondrial membrane potential and the release of cytochrome c during apoptosis...
  3. pmc Adaptive and mutational resistance: role of porins and efflux pumps in drug resistance
    Lucía Fernández
    Centre for Microbial Diseases and Immunity Research, Department of Microbiology and Immunology, University of British Columbia, Vancouver, British Columbia, Canada
    Clin Microbiol Rev 25:661-81. 2012
    ..This review evaluates the impact of porins and efflux pumps on two major types of resistance, namely, mutational and adaptive types of resistance, both of ..
  4. ncbi Coding sequence targeting by MicC RNA reveals bacterial mRNA silencing downstream of translational initiation
    Verena Pfeiffer
    Max Planck Institute for Infection Biology, RNA Biology Group, Berlin, Germany
    Nat Struct Mol Biol 16:840-6. 2009
    ..The discovery of CDS targeting markedly expands the sequence space for sRNA target predictions in bacteria...
  5. pmc Outer membrane permeability and antibiotic resistance
    Anne H Delcour
    Department of Biology, University of Houston, 369 Science and Research Building II, Houston, TX 77204 5001, USA
    Biochim Biophys Acta 1794:808-16. 2009
    ..can take through the outer membrane: a lipid-mediated pathway for hydrophobic antibiotics, and general diffusion porins for hydrophilic antibiotics...
  6. ncbi A small non-coding RNA of the invasion gene island (SPI-1) represses outer membrane protein synthesis from the Salmonella core genome
    Verena Pfeiffer
    Max Planck Institute for Infection Biology, Chariteplatz 1, D 10117 Berlin, Germany
    Mol Microbiol 66:1174-91. 2007
    ..This study identifies the first regulatory RNA of an enterobacterial pathogenicity island, and new roles for Hfq and HilD in SPI-1 gene expression...
  7. pmc Klebsiella pneumoniae outer membrane porins OmpK35 and OmpK36 play roles in both antimicrobial resistance and virulence
    Yu Kuo Tsai
    Division of Infectious Diseases, National Health Research Institutes, Miaoli, Taiwan
    Antimicrob Agents Chemother 55:1485-93. 2011
    OmpK35 and OmpK36 are the major outer membrane porins of Klebsiella pneumoniae. In this study, a virulent clinical isolate was selected to study the role of these two porins in antimicrobial resistance and virulence...
  8. ncbi Porins, efflux pumps and multidrug resistance in Acinetobacter baumannii
    Jordi Vila
    Servei de Microbiologia, Centre de Diagnostic Biomedic, Hospital Clinic, IDIBAPS, Facultat de Medicina, Universitat de Barcelona, Barcelona, Spain
    J Antimicrob Chemother 59:1210-5. 2007
    ..acquisition of genetic elements carrying multiple resistant determinants or mutations affecting the expression of porins and/or efflux pump(s), which can affect unrelated antimicrobial agents...
  9. ncbi The porin and the permeating antibiotic: a selective diffusion barrier in Gram-negative bacteria
    Jean Marie Pages
    UMR MD 1, Transporteurs Membranaires, Chimioresistance et Drug Design, Facultés de Médecine et de harmacie, Universite de la Mediterranee, Marseille, 13385, France
    Nat Rev Microbiol 6:893-903. 2008
    ..The outer membrane contains various protein channels, called porins, which are involved in the influx of various compounds, including several classes of antibiotics...
  10. ncbi Sus1, a functional component of the SAGA histone acetylase complex and the nuclear pore-associated mRNA export machinery
    Susana Rodriguez-Navarro
    Biochemie Zentrum der Universität Heidelberg BZH, Im Neuenheimer Feld 328, D 69120 Heidelberg, Germany
    Cell 116:75-86. 2004
    ..Finally, mRNA export is impaired in sus1 mutants. These data provide an unexpected connection between the SAGA histone acetylase complex and the mRNA export machinery...
  11. pmc Identification and localization of Myxococcus xanthus porins and lipoproteins
    Swapna Bhat
    Department of Microbiology, University of Georgia, Athens, Georgia, United States of America
    PLoS ONE 6:e27475. 2011
    ..These results suggest that ECM proteins have a unique method of secretion. It is now possible to target lipoproteins to specific IM, OM and ECM locations by manipulating the amino acid sequence near the +1 cysteine processing site...
  12. ncbi Crystal structures of various maltooligosaccharides bound to maltoporin reveal a specific sugar translocation pathway
    R Dutzler
    Department of Structural Biology, Biozentrum, University of Basel, CH 4056 Basel, Switzerland
    Structure 4:127-34. 1996
    ..coli. In particular, it is indispensable for the transport of long maltooligosaccharides, as these do not pass through non-specific porins. An understanding of this intriguing capability requires elucidation of the structural basis.
  13. pmc Single-virus tracking reveals a spatial receptor-dependent search mechanism
    Eli Rothenberg
    Department of Physics, University of Illinois at Urbana Champaign, Urbana, Illinois, USA
    Biophys J 100:2875-82. 2011
    ..We discuss the possible implications of our findings for the process of viral receptor-finding in higher systems...
  14. ncbi Complex spatial distribution and dynamics of an abundant Escherichia coli outer membrane protein, LamB
    Karine A Gibbs
    Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA 94305, USA
    Mol Microbiol 53:1771-83. 2004
    ..In this study, we have demonstrated that LamB moves along the bacterial surface and that these movements are restricted by an underlying dynamic spiral pattern...
  15. pmc Vaccination with the Chlamydia trachomatis major outer membrane protein can elicit an immune response as protective as that resulting from inoculation with live bacteria
    Sukumar Pal
    Department of Pathology and Laboratory Medicine, Medical Sciences I, Room D440, University of California, Irvine, Irvine, CA 92697 4800, USA
    Infect Immun 73:8153-60. 2005
    ..05). In conclusion, immunization with a purified preparation of the MOMP is as effective as vaccination with viable C. trachomatis in eliciting a protective immune response against a genital challenge in mice...
  16. pmc Use of OmpU porins for attachment and invasion of Crassostrea gigas immune cells by the oyster pathogen Vibrio splendidus
    Marylise Duperthuy
    Institut Francais de Recherche pour l Exploitation de la Mer, Centre National de la Recherche Scientifique, Universite Montpellier 2, 34095 Montpellier, France
    Proc Natl Acad Sci U S A 108:2993-8. 2011
    OmpU porins are increasingly recognized as key determinants of pathogenic host Vibrio interactions...
  17. ncbi The growth rate of Mycobacterium smegmatis depends on sufficient porin-mediated influx of nutrients
    Joachim Stephan
    Lehrstuhl fur Mikrobiologie, Friedrich Alexander Universitat Erlangen Nurnberg, Staudtstrasse 5, D 91058 Erlangen, Germany
    Mol Microbiol 58:714-30. 2005
    ..Nutrients cross this permeability barrier by diffusion through porins. MspA is the major porin of Mycobacterium smegmatis...
  18. ncbi Nab2p and the Thp1p-Sac3p complex functionally interact at the interface between transcription and mRNA metabolism
    Mercedes Gallardo
    Departamento de Genetica, Facultad de Biologia, Universidad de Sevilla, Avenida Reina Mercedes 6, 41012 Sevilla, Spain
    J Biol Chem 278:24225-32. 2003
    ..Altogether, our results suggest that Thp1p-Sac3p and Nab2p are functionally related heterogeneous nuclear ribonucleoproteins that define a further link between mRNA metabolism and transcription...
  19. ncbi Structural and dynamical properties of the porins OmpF and OmpC: insights from molecular simulations
    Amit Kumar
    Istituto Officina dei Materiali CNR UOS SLACS, c o Department of Physics, University of Cagliari, SP Monserrato Sestu Km 0 700, Monserrato I 09042, Italy
    J Phys Condens Matter 22:454125. 2010
    In this paper we investigate the structural and dynamical properties of the two major porins (OmpF and OmpC) in Escherichia coli, using molecular dynamics (MD) simulations...
  20. pmc Silver-resistant mutants of Escherichia coli display active efflux of Ag+ and are deficient in porins
    X Z Li
    Department of Microbiology, University of Saskatchewan, Saskatoon, Canada
    J Bacteriol 179:6127-32. 1997
    ..outer membrane (OM) permeability to cephalosporins, and all five resistant mutants tested were deficient in major porins, either OmpF or OmpF plus OmpC...
  21. ncbi The murine voltage-dependent anion channel gene family. Conserved structure and function
    M J Sampson
    Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030, USA
    J Biol Chem 272:18966-73. 1997
    ..VDAC3, however, is only able to partially complement the mutant phenotype, suggesting an alternative physiologic function for the VDAC3 protein...
  22. ncbi Complex inhibition of OmpF and OmpC bacterial porins by polyamines
    R Iyer
    Department of Biology, University of Houston, Houston, Texas 77204 5513, USA
    J Biol Chem 272:18595-601. 1997
    The effects of four polyamines (putrescine, cadaverine, spermidine, and spermine) on the activity of bacterial porins OmpC and OmpF were investigated by electrophysiology...
  23. ncbi Structure of the complex of the colicin E2 R-domain and its BtuB receptor. The outer membrane colicin translocon
    Onkar Sharma
    Department of Biological Sciences, Lilly Hall of Life Sciences, Purdue University, West Lafayette, Indiana 47907, USA
    J Biol Chem 282:23163-70. 2007
    ..Arg(452) at the R/C-domain interface in colicin E2 was found have an essential role at a putative site of protease cleavage, which would liberate the C-terminal activity domain for passage through the outer membrane translocon...
  24. pmc MicC, a second small-RNA regulator of Omp protein expression in Escherichia coli
    Shuo Chen
    Ibis Therapeutics, Isis Pharmaceuticals, Inc, Carlsbad, California, USA
    J Bacteriol 186:6689-97. 2004
    ..We suggest that the MicF and MicC RNAs act in conjunction with the EnvZ-OmpR two-component system to control the OmpF/OmpC protein ratio in response to a variety of environmental stimuli...
  25. pmc Role of porins in iron uptake by Mycobacterium smegmatis
    Christopher M Jones
    Department of Microbiology, University of Alabama at Birmingham, 609 Bevill Biomedical Research Building, 845 19th Street South, Birmingham, Alabama 35294, USA
    J Bacteriol 192:6411-7. 2010
    ..of iron in Gram-negative bacteria includes diffusion of iron ions or chelates across the outer membrane through porins. The outer membrane of the saprophytic Mycobacterium smegmatis contains the Msp family of porins, which enable the ..
  26. pmc Sequence polymorphism, predicted secondary structures, and surface-exposed conformational epitopes of Campylobacter major outer membrane protein
    Q Zhang
    Food Animal Health Research Program, The Ohio State University, Wooster, Ohio 44691, USA
    Infect Immun 68:5679-89. 2000
    ..These findings are instrumental in the design of MOMP-based diagnostic tools and vaccines...
  27. pmc Surface expression, single-channel analysis and membrane topology of recombinant Chlamydia trachomatis Major Outer Membrane Protein
    Heather E Findlay
    Division of Biomedical Sciences, University of Edinburgh Medical School, George Square, Edinburgh EH8 9XD, UK
    BMC Microbiol 5:5. 2005
    ..coli cells, it often misfolds and aggregates. We aimed to improve the surface expression of correctly folded MOMP to investigate the membrane topology of the protein, and provide a system to display native and modified MOMP epitopes...
  28. pmc High-level carbapenem resistance in a Klebsiella pneumoniae clinical isolate is due to the combination of bla(ACT-1) beta-lactamase production, porin OmpK35/36 insertional inactivation, and down-regulation of the phosphate transport porin phoe
    Frank M Kaczmarek
    Pfizer Global Research and Development, Groton, CT 06340, USA
    Antimicrob Agents Chemother 50:3396-406. 2006
    ..Our results with this pair of Klebsiella clinical isolates highlight the complex and evolving nature of multiple drug resistance in this species...
  29. pmc Caught at its own game: regulatory small RNA inactivated by an inducible transcript mimicking its target
    Nara Figueroa-Bossi
    Centre de Genetique Moleculaire, Centre National de la Recherche Scientifique CNRS, Gif sur Yvette 91198, France
    Genes Dev 23:2004-15. 2009
    ..Overall, our findings blur the distinction between effector and target in sRNA regulation, raising the possibility that some of the currently defined targets could actually be inhibitors of sRNA function...
  30. pmc Site-directed mutagenesis of tyrosine 118 within the central constriction site of the LamB (maltoporin) channel of Escherichia coli. II. Effect on maltose and maltooligosaccharide binding kinetics
    Frank Orlik
    Lehrstuhl fur Biotechnologie, Theodor Boveri Institut Biozentrum der Universität Würzburg, Am Hubland, D 97074 Wurzburg, Germany
    Biophys J 83:309-21. 2002
    ..The results suggest that tyrosine 118 has a crucial effect on carbohydrate transport through LamB...
  31. pmc Review of meningococcal group B vaccines
    Dan M Granoff
    Center for Immunobiology and Vaccine Development, Children s Hospital Oakland Research Institute, Oakland, California, USA
    Clin Infect Dis 50:S54-65. 2010
    ..Thus, the prospects have never been better for developing vaccines for prevention of meningococcal disease, including that caused by group B strains...
  32. ncbi A putative alpha-helical porin from Corynebacterium glutamicum
    Karin Ziegler
    Institut fur Organische Chemie und Biochemie, Albert Ludwigs Universitat, Albertstr 21, 79104 Freiburg im Breisgau, Germany
    J Mol Biol 379:482-91. 2008
    ..The exchange of material across this barrier requires porins. Porin B (PorB) is one of them...
  33. ncbi Crystal structure of Omp32, the anion-selective porin from Comamonas acidovorans, in complex with a periplasmic peptide at 2.1 A resolution
    K Zeth
    Max Planck Institut fur Biochemie, Abteilung Molekulare Strukturbiologie, Am Klopferspitz 18a, D 82152, Martinsried, Germany
    Structure 8:981-92. 2000
    b>Porins provide diffusion channels for salts and small organic molecules in the outer membrane of bacteria...
  34. pmc The yeast nucleoporin Nup53p specifically interacts with Nic96p and is directly involved in nuclear protein import
    B Fahrenkrog
    M E Müller Institute for Structural Biology, Biozentrum, University of Basel, CH 4056 Basel, Switzerland
    Mol Biol Cell 11:3885-96. 2000
    ..Whereas Nup53p is directly involved in NLS-mediated protein import by its interaction with the yeast nuclear import receptor Kap95p, it appears not to participate in NES-dependent nuclear export...
  35. pmc Long-term evolution of antigen repertoires among carried meningococci
    Caroline O Buckee
    Department of Zoology, University of Oxford, Oxford OX1 3PS, UK
    Proc Biol Sci 277:1635-41. 2010
    ..We contrast this antigenic structure with the overlapping but relatively stable combinations of the housekeeping genes observed among the same isolates, and use a novel network approach to visualize these relationships...
  36. pmc The motion of a single molecule, the lambda-receptor, in the bacterial outer membrane
    Lene Oddershede
    The Niels Bohr Institute, University of Copenhagen, Blegdamsvej 17, 2100 Copenhagen Ø, Denmark
    Biophys J 83:3152-61. 2002
    ..0 x 10(-2) pN/nm to the bacterial membrane. The purpose of the protein motion might be to facilitate transport of maltodextrins through the outer bacterial membrane...
  37. ncbi Structure of the sucrose-specific porin ScrY from Salmonella typhimurium and its complex with sucrose
    D Forst
    Institut fur Biophysik und Strahlenbiologie, Albert Ludwigs Universitat, Freiburg im Breisgau, Germany
    Nat Struct Biol 5:37-46. 1998
    ..Two non-overlapping sucrose-binding sites were identified in difference Fourier maps. The higher permeability for sucrose of ScrY as compared to maltoporin is mainly accounted for by differences in their pore-lining residues...
  38. pmc Implication of porins in beta-lactam resistance of Providencia stuartii
    Que Tien Tran
    School of Engineering and Science, Jacobs University Bremen, D 28759 Bremen, Germany
    J Biol Chem 285:32273-81. 2010
    ..coli cells to β-lactams compared with expression of OmpPst2. All our data enlighten the involvement of porins in the resistance of P. stuartii to β-lactam antibiotics.
  39. ncbi Down-regulation of porins by a small RNA bypasses the essentiality of the regulated intramembrane proteolysis protease RseP in Escherichia coli
    Véronique Douchin
    Signalisation et Réseaux de Régulations Bactériens, Institut de Genetique et Microbiologie, CNRS UMR8621 IFR115, Centre Scientifique d Orsay, Universite Paris Sud, 91405 Orsay Cedex, France
    J Biol Chem 281:12253-9. 2006
    ....
  40. pmc Porins from Salmonella enterica serovar Typhimurium activate the transcription factors activating protein 1 and NF-kappaB through the Raf-1-mitogen-activated protein kinase cascade
    Massimiliano Galdiero
    Istituto di Microbiologia, Facolta di Medicina e Chirurgia, Seconda Universita degli Studi di Napoli, 80138 Naples, Italy
    Infect Immun 70:558-68. 2002
    In this study we examined the ability of Salmonella enterica serovar Typhimurium porins to activate activating protein 1 (AP-1) and nuclear factor kappaB (NF-kappaB) through the mitogen-activated protein kinase (MAPK) cascade, and we ..
  41. ncbi The role of mitochondrial porins and the permeability transition pore in learning and synaptic plasticity
    Edwin J Weeber
    Division of Neuroscience, Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030, USA
    J Biol Chem 277:18891-7. 2002
    Mitochondrial outer membrane permeability is conferred by a family of porin proteins. Mitochondrial porins conduct small molecules and constitute one component of the permeability transition pore that opens in response to apoptotic ..
  42. pmc Role of surface-exposed loops of Haemophilus influenzae protein P2 in the mitogen-activated protein kinase cascade
    Stefania Galdiero
    Dipartimento di Chimica Biologica, Università degli Studi di Napoli Federico II and Istituto di Biostrutture e Bioimmagini, CNR, 80134 Naples, Italy
    Infect Immun 71:2798-809. 2003
    The outer membrane of gram-negative bacteria contains several proteins, and some of these proteins, the porins, have numerous biological functions in the interaction with the host; porins are involved in the activation of signal ..
  43. ncbi ATP flux is controlled by a voltage-gated channel from the mitochondrial outer membrane
    T Rostovtseva
    Laboratories of Cell Biology, Department of Zoology, University of Maryland, College Park, Maryland 20742, USA
    J Biol Chem 271:28006-8. 1996
    ..Thus, these channels are ideally suited to control ATP flux through the mitochondrial outer membrane and, consequently, mitochondrial function. The block to ATP flow through the closed state is likely to be not steric but electrostatic...
  44. ncbi OMP peptide signals initiate the envelope-stress response by activating DegS protease via relief of inhibition mediated by its PDZ domain
    Nathan P Walsh
    Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Cell 113:61-71. 2003
    ..Escherichia coli respond to misfolded or unfolded outer-membrane porins (OMPs) in the periplasm by inducing sigma(E)-dependent transcription of stress genes in the cytoplasm...
  45. pmc Single-molecule DNA detection with an engineered MspA protein nanopore
    Tom Z Butler
    Department of Physics, University of Washington, Box 351560, 3910 15th Avenue NE, Seattle, WA 98195, USA
    Proc Natl Acad Sci U S A 105:20647-52. 2008
    ..Our results introduce MspA as a nanopore for nucleic acid analysis and highlight its potential as an engineerable platform for single-molecule detection and characterization applications...
  46. pmc Mobility of BtuB and OmpF in the Escherichia coli outer membrane: implications for dynamic formation of a translocon complex
    Jeff Spector
    Department of Physics, Purdue University, West Lafayette, Indiana, USA
    Biophys J 99:3880-6. 2010
    ..Thus, formation of the outer membrane translocon for cellular import of the nuclease colicins is a demonstrably dynamic process, because it depends on lateral diffusion of BtuB and collisional interaction with relatively immobile OmpF...
  47. ncbi Maintaining a healthy SPANC balance through regulatory and mutational adaptation
    Thomas Ferenci
    School of Molecular and Microbial Biosciences, The University of Sydney, NSW 2006, Australia
    Mol Microbiol 57:1-8. 2005
    ..selective pressures working in opposite directions (antagonistic pleiotropy) result in polymorphisms affecting porins and RpoS...
  48. ncbi Recognition of heptameric seed sequence underlies multi-target regulation by RybB small RNA in Salmonella enterica
    Roberto Balbontin
    Departamento de Genetica, Facultad de Biologia, Universidad de Sevilla, 41080 Seville, Spain
    Mol Microbiol 78:380-94. 2010
    ..analysis to dissect the functional anatomy of RybB, a σ(E)-dependent sRNA that regulates the synthesis of major porins in Escherichia coli and Salmonella...
  49. ncbi Cutting edge: Immune stimulation by neisserial porins is toll-like receptor 2 and MyD88 dependent
    Paola Massari
    Department of Medicine, Division of Infectious Diseases, Boston University School of Medicine, Boston, MA 02118, USA
    J Immunol 168:1533-7. 2002
    The immunopotentiating activity of neisserial porins, the major outer membrane protein of the pathogenic Neisseria, is mediated by its ability to stimulate B cells and up-regulate the surface expression of B7-2...
  50. pmc The VR2 epitope on the PorA P1.7-2,4 protein is the major target for the immune response elicited by the strain-specific group B meningococcal vaccine MeNZB
    D R Martin
    Insitute of Environmental Science and Research ESR, Porirua, New Zealand
    Clin Vaccine Immunol 13:486-91. 2006
    ..4 epitope of the PorA protein supports the public health decision to use a strain-specific vaccine for the control of New Zealand's epidemic of meningococcal disease...
  51. ncbi Structure and function of bacterial outer membrane proteins: barrels in a nutshell
    R Koebnik
    Biozentrum Basel, Abteilung Mikrobiologie, Klingelbergstr 50, CH 4056 Basel, Switzerland
    Mol Microbiol 37:239-53. 2000
    ..They include the OmpA membrane domain, the OmpX protein, phospholipase A, general porins (OmpF, PhoE), substrate-specific porins (LamB, ScrY) and the TonB-dependent iron siderophore transporters FhuA and ..
  52. ncbi Molecular simulations reveal the mechanism and the determinants for ampicillin translocation through OmpF
    Amit Kumar
    Department of Physics, University of Cagliari and Istituto Officina dei Materiali CNR UOS SLACS, I 09042 Monserrato Ca, Italy
    J Phys Chem B 114:9608-16. 2010
    ..As a whole, our analysis opens the way to chemical modifications of antibiotics to allow improving uptake through porins contributing to combat bacterial resistance.
  53. pmc Differential expression of the OmpF and OmpC porin proteins in Escherichia coli K-12 depends upon the level of active OmpR
    C Y Lan
    Division of Biological Sciences, University of California, Davis, 95616, USA
    J Bacteriol 180:171-4. 1998
    ..This result supports a key prediction of the current model of porin regulation, which states that the differential regulation of OmpF and OmpC is a direct consequence of the cellular level of the active form of OmpR...
  54. ncbi Transcription regulation of ompF and ompC by a single transcription factor, OmpR
    Takeshi Yoshida
    Department of Biochemistry, Robert Wood Johnson Medical School, Piscataway, New Jersey 08854, USA
    J Biol Chem 281:17114-23. 2006
    ....
  55. pmc Role of porins for uptake of antibiotics by Mycobacterium smegmatis
    Olga Danilchanka
    Department of Microbiology, University of Alabama at Birmingham, Alabama 35294, USA
    Antimicrob Agents Chemother 52:3127-34. 2008
    ..Deletion of the porins MspA and MspC drastically increased the resistance of M...
  56. pmc Roles of beta-lactamases and porins in activities of carbapenems and cephalosporins against Klebsiella pneumoniae
    L Martinez-Martinez
    Department of Microbiology, School of Medicine, University of Seville, Seville, Spain
    Antimicrob Agents Chemother 43:1669-73. 1999
    ..provided resistance to imipenem (up to 64 microg/ml) and meropenem (up to 16 microg/ml) in strains deficient in porins. Carbapenem resistance showed little inoculum effect, was not affected by clavulanate but was blocked by BRL 42715,..
  57. ncbi The voltage-dependent anion channel is a receptor for plasminogen kringle 5 on human endothelial cells
    Mario Gonzalez-Gronow
    Department of Pathology, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Biol Chem 278:27312-8. 2003
    ..K5 binding to endothelial cells also induces a decrease in intracellular pH and hyperpolarization of the mitochondrial membrane. These studies suggest that VDAC is a receptor for K5...
  58. ncbi Structural basis for sugar translocation through maltoporin channels at 3.1 A resolution
    T Schirmer
    Department of Structural Biology, University of Basel, Switzerland
    Science 267:512-4. 1995
    ....
  59. ncbi MspA provides the main hydrophilic pathway through the cell wall of Mycobacterium smegmatis
    C Stahl
    Lehrstuhl fur Mikrobiologie, Friedrich Alexander Universitat Erlangen Nurnberg, Staudtstr 5, 91058 Erlangen, Germany
    Mol Microbiol 40:451-64. 2001
    ..smegmatis and was only partially replaced by fewer porins in the cell wall of the DeltamspA mutant [corrected] This is the first experimental evidence that porins are the ..
  60. ncbi Emerging issues in the management of infections caused by multidrug-resistant gram-negative bacteria
    Louis B Rice
    Louis Stokes Cleveland VA Medical Center and Case Western Reserve University School of Medicine, Cleveland, OH 44106, USA
    Cleve Clin J Med 74:S12-20. 2007
    ....
  61. pmc Essential role of voltage-dependent anion channel in various forms of apoptosis in mammalian cells
    S Shimizu
    Osaka University Graduate School of Medicine, Biomedical Research Center, Department of Medical Genetics, Osaka 565-0871, Japan
    J Cell Biol 152:237-50. 2001
    ..Taken together, our data provide evidence that the VDAC plays an essential role in apoptogenic cytochrome c release and apoptosis in mammalian cells...
  62. pmc Genetic diversity and mosaicism at the por locus of Neisseria gonorrhoeae
    T C Fudyk
    Department of Medical Microbiology, University of Manitoba, Winnipeg, Manitoba, Canada
    J Bacteriol 181:5591-9. 1999
    ..Despite extensive sequence variability, por expressed by gonococcal isolates of different geographic origin exhibited conserved patterns of nucleotide change, suggesting that diversity among por alleles may also be finite...
  63. ncbi Immunogenic characterization of outer membrane porins OmpC and OmpF of porcine extraintestinal pathogenic Escherichia coli
    Canying Liu
    State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China
    FEMS Microbiol Lett 337:104-11. 2012
    ..In this study, two outer membrane porins, OmpC and OmpF, of porcine ExPEC were cloned and expressed to investigate their immunogenicity...
  64. pmc Sequence variation in the porA gene of a clone of Neisseria meningitidis during epidemic spread
    J Jelfs
    WHO Collaborating Centre for Reference and Research on Meningococci, Department of Bacteriology, National Institute of Public Health, N 0403 Oslo, Norway
    Clin Diagn Lab Immunol 7:390-5. 2000
    ..The magnitude of microevolutionary mechanisms available in meningococci and the remarkable genetic flexibility of these bacteria need to be considered in relation to PorA vaccine development...
  65. pmc Functional comparison of the binding of factor H short consensus repeat 6 (SCR 6) to factor H binding protein from Neisseria meningitidis and the binding of factor H SCR 18 to 20 to Neisseria gonorrhoeae porin
    Jutamas Shaughnessy
    Division of Infectious Diseases and Immunology, University of Massachusetts Medical School, Lazare Research Building, Room 370I, Plantation Street, Worcester, MA 01605, USA
    Infect Immun 77:2094-103. 2009
    ..Differences in fH binding to these two related pathogens may be important for modulating their individual responses to host immune attack...
  66. pmc Amino acids important for DNA recognition by the response regulator OmpR
    Jee Eun Rhee
    Department of Microbiology and Immunology, University of Illinois, Chicago, IL 60612, USA
    J Biol Chem 283:8664-77. 2008
    ..The flexible linker of OmpR enables the second monomer to bind in multiple orientations (head-to-tail and head-to-head), depending on the specific DNA contacts...
  67. ncbi The structure of BtuB with bound colicin E3 R-domain implies a translocon
    Genji Kurisu
    Department of Biological Sciences, Purdue University, Lilly Hall of Life Sciences, 915 W State St, West Lafayette, Indiana 47907 1392, USA
    Nat Struct Biol 10:948-54. 2003
    ..The translocator was concluded to be OmpF from the occlusion of OmpF channels by colicin E3...
  68. pmc Defining targets for complement components C4b and C3b on the pathogenic neisseriae
    Lisa A Lewis
    Division of Infectious Diseases and Immunology, University of Massachusetts Medical School, Lazare Research Building, Room 370I, 364 Plantation Street, Worcester, MA 01605, USA
    Infect Immun 76:339-50. 2008
    ..The binding of C3b and C4b to Opa provides a rationale for the recovery of predominantly "transparent" (Opa-negative) neisserial isolates from persons with invasive disease, where the bacteria encounter high levels of complement...
  69. ncbi Chlamydia trachomatis infection: incidence, health costs and prospects for vaccine development
    K W Beagley
    Discipline of Immunology and Microbiology, Faculty of Medicine and Health Sciences, University of Newcastle, NSW 2308, Newcastle, Australia
    J Reprod Immunol 48:47-68. 2000
    ..Thus there are many challenges for the development of a successful vaccine which must elicit immunity against multiple serovars while at the same time minimising damaging pro-inflammatory immune responses...
  70. pmc Understanding ion conductance on a molecular level: an all-atom modeling of the bacterial porin OmpF
    Soroosh Pezeshki
    School of Engineering and Science, Jacobs University Bremen, Bremen, Germany
    Biophys J 97:1898-906. 2009
    ..Reduction of the negatively charged residues decreases the conductance by half, whereas cationic residues enhance the conductance. Experiments on mutants confirm the results of the molecular-level simulations...
  71. ncbi VDAC regulation by the Bcl-2 family of proteins
    Y Tsujimoto
    Department of Medical Genetics, Biomedical Research Center, Osaka University Graduate School of Medicine, CREST of Japan Science and Technology Corp JST, Suita, Osaka, Japan
    Cell Death Differ 7:1174-81. 2000
    ..Cell Death and Differentiation (2000) 7, 1174 - 1181..
  72. pmc Protection against an intranasal challenge by vaccines formulated with native and recombinant preparations of the Chlamydia trachomatis major outer membrane protein
    Guifeng Sun
    Department of Pathology and Laboratory Medicine, Medical Sciences, Room D440, University of California, Irvine, CA 92697 4800, United States
    Vaccine 27:5020-5. 2009
    ..05). In conclusion, vaccination with a preparation of the nMOMP elicited a more robust protection than immunization with rMOMP, suggesting that the conformational structure of MOMP is critical for inducing strong protection...
  73. ncbi Serum bactericidal activity and isotype distribution of antibodies in toddlers and schoolchildren after vaccination with RIVM hexavalent PorA vesicle vaccine
    E de Kleijn
    Department of Pediatrics, Sophia Children's Hospital/University Hospital, Rotterdam, The Netherlands
    Vaccine 20:352-8. 2001
    ..The isotype distribution of the response is not age-dependent...
  74. ncbi VrrA mediates Hfq-dependent regulation of OmpT synthesis in Vibrio cholerae
    Tianyan Song
    Department of Molecular Biology, The Laboratory for Molecular Infection Medicine Sweden, Umeå Center for Microbial Research, Umea University, SE 901 87 Umea, Sweden
    J Mol Biol 400:682-8. 2010
    ..In addition to pH/temperature signals via the ToxR regulon and carbon source signals via the cAMP-CRP complex, OmpT is further regulated by signals received via the sigma(E) regulon through VrrA...
  75. ncbi Identification and sequence analysis of Treponema pallidum tprJ, a member of a polymorphic multigene family
    L V Stamm
    Department of Epidemiology, School of Public Health, University of North Carolina, Chapel Hill 27599 7400, USA
    FEMS Microbiol Lett 169:155-63. 1998
    ..The deduced amino acid sequence of T. pertenue TprJ is 87.3% identical to that of T. pallidum TprJ. This is the first report of significant sequence differences within homologous genes of T. pallidum and T. pertenue...
  76. pmc Directed epitope delivery across the Escherichia coli outer membrane through the porin OmpF
    Nicholas G Housden
    Department of Biology, Area 10, and York Structural Biology Laboratory, University of York, Heslington, York YO10 5DD, United Kingdom
    Proc Natl Acad Sci U S A 107:21412-7. 2010
    The porins OmpF and OmpC are trimeric β-barrel proteins with narrow channels running through each monomer that exclude molecules > 600 Da while mediating the passive diffusion of small nutrients and metabolites across the Gram-..
  77. pmc Computational electrophysiology: the molecular dynamics of ion channel permeation and selectivity in atomistic detail
    Carsten Kutzner
    Department of Theoretical and Computational Biophysics, Max Planck Institute for Biophysical Chemistry, Gottingen, Germany
    Biophys J 101:809-17. 2011
    ..We show that our method accurately predicts ion conductance and selectivity and elucidates ion conduction mechanisms in great detail. Handles for overcoming channel-related antibiotic resistance are identified...
  78. ncbi Site-directed mutagenesis of the greasy slide aromatic residues within the LamB (maltoporin) channel of Escherichia coli: effect on ion and maltopentaose transport
    Katrin Denker
    Lehrstuhl fur Biotechnologie, Theodor Boveri Institut Biozentrum der Universität Würzburg, Am Hubland, D 97074 Wurzburg, Germany
    J Mol Biol 352:534-50. 2005
    ..For most mutants, k1 decreased and k(-1) increased. The possible influence of the aromatic residues of the greasy slide on carbohydrate and ion transport through LamB is discussed...
  79. ncbi Crystal structures explain functional properties of two E. coli porins
    S W Cowan
    Department of Structural Biology, University of Basel, Switzerland
    Nature 358:727-33. 1992
    b>Porins form aqueous channels that aid the diffusion of small hydrophilic molecules across the outer membrane of Gram-negative bacteria...
  80. pmc Serum immune responses predict rapid disease progression among children with Crohn's disease: immune responses predict disease progression
    Marla C Dubinsky
    Department of Pediatrics, Genetics Institute, Cedars Sinai Medical Center, Los Angeles, California, USA
    Am J Gastroenterol 101:360-7. 2006
    ..We examined the association of immune responses to microbial antigens with disease behavior and prospectively determined the influence of immune reactivity on disease progression in pediatric CD patients...
  81. ncbi Preclinical safety and immunogenicity evaluation of a nonavalent PorA native outer membrane vesicle vaccine against serogroup B meningococcal disease
    Patricia Kaaijk
    Unit Vaccinology, Centre for Infectious Disease Control CIb, National Institute for Public Health and the Environment RIVM, Bilthoven, The Netherlands
    Vaccine 31:1065-71. 2013
    ..In the present study, the safety and immunogenicity of this next-generation NonaMen vaccine were evaluated following repeated vaccination in rabbits and mice...
  82. pmc Structural basis for solute transport, nucleotide regulation, and immunological recognition of Neisseria meningitidis PorB
    Mikio Tanabe
    Department of Pharmacology, Vanderbilt University Medical Center, Nashville, TN 37232 6600, USA
    Proc Natl Acad Sci U S A 107:6811-6. 2010
    ..meningitidis and can be recognized by receptors of the host innate immune system. Features of PorB suggest that Toll-like receptor mediated recognition outer membrane proteins may be initiated with a nonspecific electrostatic attraction...
  83. ncbi Mucosal immunization with recombinant MOMP genetically linked with modified cholera toxin confers protection against Chlamydia trachomatis infection
    Shree R Singh
    Department of Mathematics and Science, Alabama State University, Montgomery, AL 36101, USA
    Vaccine 24:1213-24. 2006
    ..n. immunization of mice with rMOMP. This is a first report using genetic fusion of cholera toxin and MOMP genes and provides a novel approach for the design and development of a mucosal vaccine against Chlamydia...
  84. pmc Additive and synergistic bactericidal activity of antibodies directed against minor outer membrane proteins of Neisseria meningitidis
    Vincent E Weynants
    GlaxoSmithKline Biologicals, Rue de l Institut 89, B 1330 Rixensart, Belgium
    Infect Immun 75:5434-42. 2007
    ..With minor OMPs, this threshold is reached when more than one antigen is targeted, and this allows cross-protection...
  85. ncbi Proteomic analysis of human U937 cell line activation mediated by Haemophilus influenzae type b P2 porin and its surface-exposed loop 7
    Valeria Severino
    Department of Life Science, Second University of Naples, Via Vivaldi 43, I 81100 Caserta, Italy
    J Proteome Res 9:1050-62. 2010
    ..P2 porin, one of the best-characterized porins in terms of its functional characteristics, is the most abundant OMP in Hib and has also been shown to possess ..
  86. pmc Stability of PorA during a meningococcal disease epidemic
    A F Devoy
    Communicable Disease Group, Institute of Environmental Science and Research, Porirua, New Zealand
    J Clin Microbiol 43:832-7. 2005
    ..7-2,4 PorA protein of meningococci responsible for New Zealand's epidemic has remained relatively stable over 13 years and support the use of a strain-specific outer membrane vesicle vaccine to control the epidemic...
  87. ncbi A tetrameric porin limits the cell wall permeability of Mycobacterium smegmatis
    Harald Engelhardt
    Max Planck Institut fur Biochemie, Abteilung Molekulare Strukturbiologie, Am Klopferspitz 18a, D 82152 Martinsried, Germany
    J Biol Chem 277:37567-72. 2002
    ..Thus, MspA is drastically different from the trimeric porins of Gram-negative bacteria and represents a new class of channel proteins...
  88. pmc Insights into pilus assembly and secretion from the structure and functional characterization of usher PapC
    Yihua Huang
    Howard Hughes Medical Institute and Department of Biochemistry, University of Texas Southwestern Medical Center, 6001 Forest Park Road, Dallas, TX 75390, USA
    Proc Natl Acad Sci U S A 106:7403-7. 2009
    ..The plug domain has a dual function: gating the beta-pore and participating in pilus assembly...
  89. ncbi Specificity of the glucose channel formed by protein D1 of Pseudomonas aeruginosa
    J Trias
    Department of Microbiology and Immunology, University of California, Berkeley 94720
    Biochim Biophys Acta 938:493-6. 1988
    ..This finding and the permeation rates of various glucose analogs suggest that, although the channel has a specific recognition site for glucose, it functions in a manner very different from that of the glucose carrier of erythrocytes...
  90. ncbi Structure of maltoporin from Salmonella typhimurium ligated with a nitrophenyl-maltotrioside
    J E Meyer
    Institut für Organische Chemie und Biochemie Albert Ludwigs Universität, Freiburg im Breisgau Germany
    J Mol Biol 266:761-75. 1997
    ..with the structure of the homologous porin from Escherichia coli as well as with the sequences of other related porins showed that there are regions of appreciable sequence and structure variability, despite close overall similarity...
  91. ncbi Maltodextrin transport through lamb
    Alain Charbit
    Laboratoire de Microbiologie, Inserm U 570, Faculte de Medecine Necker, 156, rue de Vaugirard, 75730 Paris Cedex 15, France
    Front Biosci 8:s265-74. 2003
    ..Genetic and biochemical analyses suggest that some of the surface loops participate directly in the orientation and entry of maltooligosaccharides into the channel and, thus, control access to the binding site...
  92. pmc Genetic study of a membrane protein: DNA sequence alterations due to 17 lamB point mutations affecting adsorption of phage lambda
    J M Clement
    Unite de Programmation Moleculaire et Toxicologie Genetique, CNRS LA 271, INSERM U 163, Institut Pasteur, Paris, France
    EMBO J 2:77-80. 1983
    ..Implications of these results for the topological organization of the lambda receptor as well as possible reasons for the limited number of altered sites detected are discussed...
  93. ncbi Substrate-specific diffusion of select dicarboxylates through Chlamydia trachomatis PorB
    A Kubo
    Division of Infectious Diseases, School of Public Health, 140 Earl Warren Hall, University of California, Berkeley, CA 94720, USA
    Microbiology 147:3135-40. 2001
    Chlamydiae contain two porins, MOMP and PorB, that facilitate diffusion of solutes through the outer membrane. MOMP is a general porin that permits the diffusion of a wide variety of compounds including carbohydrates and amino acids...
  94. ncbi Porins in prokaryotes and eukaryotes: common themes and variations
    Kornelius Zeth
    Max Planck Institute of Developmental Biology, Department Protein Evolution, Spemannstr 35, D 72076 Tubingen, Germany
    Biochem J 431:13-22. 2010
    ..Despite the lack of a clear evolutionary link between these protein families, their common principles and differences in assembly, architecture and function are summarized in the present review...
  95. ncbi Antibiotic translocation through membrane channels: temperature-dependent ion current fluctuation for catching the fast events
    Kozhinjampara R Mahendran
    School of Engineering and Science, Jacobs University Bremen, Campus Ring 1, Bremen, Germany
    Eur Biophys J 38:1141-5. 2009
    ..The activation energy for ampicillin translocation through OmpF is estimated to be around 13 kT. Temperature-dependent study gives new insights into the faster translocation of small substrates through biological nanopores...
  96. pmc Crystal structures of the OmpF porin: function in a colicin translocon
    Eiki Yamashita
    Department of Biological Sciences, Purdue University, West Lafayette, IN 47907 1392, USA
    EMBO J 27:2171-80. 2008
    ..Involvement of OmpF in colicin passage through the OM was further documented by immuno-extraction of an OM complex, the colicin translocon, consisting of colicin E3, BtuB and OmpF...
  97. pmc Mutagenesis and functional reconstitution of chlamydial major outer membrane proteins: VS4 domains are not required for pore formation but modify channel function
    E S Hughes
    Department of Biomedical Sciences, University of Edinburgh, Edinburgh EH8 9XD, United Kingdom
    Infect Immun 69:1671-8. 2001
    ..If MOMPs adopt a beta-barrel fold, like bacterial porins, the VS domains may form extramembranous loops and the conserved regions of the protein may correspond to ..
  98. pmc Vibrio cholerae OmpU and OmpT porins are differentially affected by bile
    Jamie A Wibbenmeyer
    Department of Biology and Biochemistry, University of Houston, Houston, Texas 77204 5001, USA
    Infect Immun 70:121-6. 2002
    ..In order to further understand the role of the OmpT and OmpU porins in the ability of V...
  99. pmc Evidence for secretory pathway localization of a voltage-dependent anion channel isoform
    R Buettner
    Institute of Pathology, University Hospital Rheinisch Westfälische Technische Hochschule Aachen, Pauwelstrasse 30, D 52074 Aachen, Germany
    Proc Natl Acad Sci U S A 97:3201-6. 2000
    Voltage-dependent anion channels (VDACs) are pore-forming proteins (porins) that form the major pathway for movement of adenine nucleotides through the outer mitochondrial membrane...
  100. pmc OmpK26, a novel porin associated with carbapenem resistance in Klebsiella pneumoniae
    Laura García-Sureda
    Instituto Universitario de Investigaciones en Ciencias de la Salud, Universidad de las Islas Baleares, Palma de Mallorca, Spain
    Antimicrob Agents Chemother 55:4742-7. 2011
    ..Loss of the expression of the major nonspecific porins OmpK35/36 is a frequent feature in these isolates...
  101. pmc Characterization and targeted disruption of murine Nup50, a p27(Kip1)-interacting component of the nuclear pore complex
    M Smitherman
    Clinical Research, Fred Hutchinson Cancer Research Center, Seattle, Washington, 98109, USA
    Mol Cell Biol 20:5631-42. 2000
    ..We conclude that Nup50 is a nucleoporin with essential functions during mouse development...

Research Grants63

  1. Allosteric regulation of the sensory protease DegS
    RANDALL VERNON MAULDIN; Fiscal Year: 2011
    ..Under heat-shock conditions, outer membrane porins (OMP) misfold and accumulate in the periplasm...
  2. Structural and biochemical characterization of the OprD membrane protein family
    Bert van den Berg; Fiscal Year: 2011
    ..In most gram-negative bacteria such as E. coli, the majority of small molecules are taken up by porins, such as OmpF and OmpC. Porins are non-specific channels and do not bind their substrates...
  3. Computational Assembly of Beta Barrel Membrane Protein
    Jie Liang; Fiscal Year: 2013
    ..3) Develop engineering principles for designing ?-barrel porins with desirable stability, oligomerization state, and pore geometry...
  4. The interaction between outer membrane porins and toll-like receptors
    T M Iverson; Fiscal Year: 2011
    ..One set of TLR ligands, the Outer Membrane Proteins (OMPs), or porins, are transmembrane 2-barrel proteins...
  5. Neisserial Porins and Antigen Presenting Cells
    LEE MARK WETZLER; Fiscal Year: 2013
    ..Moreover, we shall further examine the mechanism of PorB adjuvant activity via the use of MyD88 floxed mice bred with mice expressing cell specific cre recombinase. ..
  6. BIOCHEMISTRY OF BACTERIAL CELL MEMBRANES
    Hiroshi Nikaido; Fiscal Year: 2013
    ..membrane of Pseudomonas aeruginosa or Acinetobacter spp, with their exceptionally low-permeability channels or porins. The molecular mechanisms that make the channel protein to show such a low permeability appear to be in the ..
  7. HTS for Cytochrome C Synthesis Pathways
    Robert G Kranz; Fiscal Year: 2011
    ..has recently shown that large molecule, heme analogs inhibit specific steps, but these analogs require specific porins for access...
  8. TRP channels in supramolecular complexes with Inorganic Polyphosphate and Polyhyd
    Eleonora Zakharian; Fiscal Year: 2013
    ..polyP/PHB complexes have been found in association with a number of membrane proteins, including ion channels and porins. Moreover, these complexes were shown to be important for normal functioning of their hosts...
  9. Markers predicting response to therapy for KPC-producing Klebsiella pneumoniae
    Yohei Doi; Fiscal Year: 2013
    ..these agents (lipid A modifications for colistin, and the expression levels of blaKPC and ompK35/36 encoding major porins for doripenem)...
  10. STRUCTURES OF SIDEROPHORE MEMBRANE TRANSPORT PROTEINS
    Dick van der Helm; Fiscal Year: 2000
    ..The complexes are too large (M.W. 800-1500) to be transported by porins. For competitive reasons microorganisms produce often more than one siderophore, and the structures of ..
  11. Porins of Mycobacterium tuberculosis
    Michael Niederweis; Fiscal Year: 2009
    ..tuberculosis by water-filled channel proteins. These porins are the key proteins for uptake of hydrophilic solutes in mycobacteria. We recently discovered two porins of M...
  12. STRUCTURE OF BACTERIAL MEMBRANE PROTEINS
    R Garavito; Fiscal Year: 1992
    The crystallization of transmembrane channel-forming proteins (porins) from the outer membrane of enteric (gram negative) bacteria (e.g., E...
  13. The OppA PEPTIDE Permease HOMOLOGUE OF ORAL SPIROCHETES
    J Fenno; Fiscal Year: 2001
    ..complements studies underway in this laboratory on assembly of membrane complexes comprised of outer membrane porins and proteases, and includes novel approaches to the study of the role of T. denticola in periodontal pathogenesis...
  14. OUTER MEMBRANE PERMEABILITY OF PSEUDOMONAS CEPACIA
    Jane Burns; Fiscal Year: 1992
    ..To examine these hypotheses, this proposal will characterize porins from isogenic permeable and impermeable strains functionally, biochemically and genetically...
  15. HOST CELL RECOGNITION OF SALMONELLA TYPHIMURIUM
    THOMAS PISTOLE; Fiscal Year: 2003
    ..The first objective is to determine whether structures found on the outer surface of Salmonella, known as porins, are involved in the recognition of this pathogen by human neutrophils...
  16. SURFACE PROTEINS OF NEISSERIA GONORRHOEAE
    Milan Blake; Fiscal Year: 1991
    ..Several different antigens are potentially good vaccine candidates such as pili and porins. However, there are others that might potentially be hazardous such as protein 111 and the lipooligosaccharides...
  17. A Genome-Derived, Epitope-Driven H. Pylori Vaccine
    Anne De Groot; Fiscal Year: 2006
    ..Epitopes derived from antigens previously shown in vivo as protective (e.g., enzymes, porins, adhesins, heat shock proteins), as well as novel epitopes from proteins not previously implicated in protection ..
  18. Environmental Modulation of ToxT-dependent Transcription
    KARL KLOSE; Fiscal Year: 2007
    ..These studies include: (i). determination of the effect of the porins OmpU and OmpT (which are known to be differentially permeable to bile) on bile modulation of ToxT activity, (ii)...
  19. QUADRUPOLE TIME OF FLIGHT MASS SPECTROMETER
    Gary Nelsestuen; Fiscal Year: 2001
    ..b>Porins of pathogenic E...
  20. STRUCTURE-FUNCTION STUDIES OF MOLECULAR CHAPERONES
    David McKay; Fiscal Year: 2007
    ..In gram negative bacteria, specific periplasmic chaperones facilitate correct maturation of outer membrane porins. Using recent information on the structure and peptide binding specificity of one of these chaperones, E...
  21. 3D POISSON-NERNST-PLANCK THEORY
    Rob Coalson; Fiscal Year: 2002
    ..equations, and, especially, to apply the method to a variety of biological systems including gramicidin, several porins, and the nicotinic acetylcholine receptor...
  22. IMMUNOPROTECTIVE POTENTIAL OF PORINS AND ANTI-PORIN MABS
    Shiva Singh; Fiscal Year: 1991
    ..abstract): This proposal deals with the study of the immunoprotective potential of Salmonella typhimurium porins and anti-porin monoclonal antibodies in experimental murine salmonellosis...
  23. Zn(II) metallochaperones in E. coli
    Michael Crowder; Fiscal Year: 2007
    ..putative, soluble Zn(ll)-metallochaperones that are thought to deliver Zn(ll) from outer membrane-bound channels/porins to the ribosomes and/or proteins. We propose to utilize the following strategy to achieve our goals...
  24. What controls the thickness of biological membranes
    Jonathan Sachs; Fiscal Year: 2006
    ..OmpF trimeric porins from E...
  25. 2008 Bacterial Cell Surfaces Gordon Research Conference
    ANNE DELCOUR; Fiscal Year: 2008
    ..Success in these research endeavors is enhanced by the exchange of information and ideas promoted by the Bacterial Cell Surfaces Gordon Conference. [unreadable] [unreadable] [unreadable]..
  26. MOLECULAR PROPERTIES AND MODULATION OF BACTERIAL PORINS
    ANNE DELCOUR; Fiscal Year: 2003
    b>Porins are large channels of the outer membrane of Gram-negative bacteria, which represent the major entry pathway for hydrophilic solutes into these organisms...
  27. Genetic screens to identify mycobacterial porins
    Martin Pavelka; Fiscal Year: 2008
    ..To facilitate uptake of hydrophilic solutes, mycobacteria, like Gram-negative organisms, utilize porins which are water-filled channels that allow passage of hydrophilic compounds such as nutrients and antibiotics ..
  28. Ligand-gated Transport through FepA
    PHILLIP KLEBBA; Fiscal Year: 2009
    ..We will use fluorescence methods to study these questions. ..
  29. Ehrilchiacidal Mechanisms In Leukocytes
    Yasuko Rikihisa; Fiscal Year: 2009
    ..The results may point to a potential target for new treatment and prevention of HME and HGE. ..
  30. GENE EXPRESSION BY HUMAN GRANULOCYTIC EHRLICHIOSIS AG
    Yasuko Rikihisa; Fiscal Year: 2004
    ..These efforts are expected to reveal an important role for the p44-multigene family in strategies of adaptive surface variation and avoidance of host immune responses. ..
  31. Molecular basis meningococcal group A capsular immunity
    Dan Granoff; Fiscal Year: 2007
    ..abstract_text> ..
  32. Neisseria meningitidis antigens expressed in infection
    Dan Granoff; Fiscal Year: 2005
    ..meningitidis disease, including group B strains for which there is currently no vaccine available. Also, characterization of gene expression in an in vivo model will lead to a better understanding of meningococcal pathogenesis. ..
  33. PATHOGENESIS OF CHLAMYDIAL OCULAR INFECTIONS
    Deborah Dean; Fiscal Year: 2004
    ..A greater understanding of the pathogenesis of trachoma will aid in developing public health interventions and a vaccine that can be implemented in trachoma endemic areas throughout the world. ..
  34. Comparison of Human Ehrlichiosis Agent Genomes
    Yasuko Rikihisa; Fiscal Year: 2010
    ..chaffeensis virulence determinants and their pathogenic mechanisms. The results may point to potential chemotherapy, chemopreventive and/or vaccine candidates for treatment and prevention of human ehrlichiosis. ..
  35. Hydrophobics transport across the outer membrane
    Bert van den Berg; Fiscal Year: 2009
    ..These structures will also allow us to determine whether mechanistic differences exist between LCFA and xenobiotics transport. ..
  36. Folding and design of beta sheets in membranes
    William C Wimley; Fiscal Year: 2010
    ..This information will be used to create a public database of potential outer membrane proteins ..
  37. C. trachomatis genomics, strain typing, and evolution
    Deborah Dean; Fiscal Year: 2008
    ..unreadable] [unreadable]..
  38. STRUCTURE BASED DRUG DESIGN
    Robert Stroud; Fiscal Year: 2004
    ..Public domain access to the structures of therapeutic targets and technology will encourage their use by major pharmaceutical companies for the better design of therapeutics. ..
  39. SIGNAL SEQUENCE RECOGNITION AND PROTEIN TARGETING
    Robert Stroud; Fiscal Year: 2007
    ..These will instruct in the mechanism of replacing the role of RNA by protein, and therefore in the essence of the dynamic roles of SRP RNA and cpSRP43. ..
  40. THYMIDYLATE SYNTHASE
    Robert Stroud; Fiscal Year: 2001
    ..Irwin Kunts, UCSF. Some mechanistic question will be approached using NMR spectroscopy in collaboration with Dr. Thomas James, UCSF. ..
  41. Molecular Basis for Transmembrane Conduction & Signaling
    Robert Stroud; Fiscal Year: 2006
    ..A further aim is to improve crystals of the Acetylcholine receptor as a means of three-dimensional structure determination of an archetype of gating mechanisms in an archetype of neuroreceptor superfamilies. ..
  42. Pathogenesis of Persistent Chlamydial STDs
    Deborah Dean; Fiscal Year: 2007
    ..1 Description, ..
  43. Plasmid-Mediated Quinolone Resistance
    George Jacoby; Fiscal Year: 2007
    ..unreadable] [unreadable]..
  44. ROLES OF EHRLICHIAL OUTER MEMBRANE PROTEIN IN PARASITISM
    Yasuko Rikihisa; Fiscal Year: 2001
    ..Aim 5 addresses hypothesis 3 by examining the effects of immunization with the ehrlichial OMP in a mouse model of human ehrlichiosis. ..
  45. 2005 Mechanisms of Membrane Transport Gordon Conference
    Robert Stroud; Fiscal Year: 2005
    ..abstract_text> ..
  46. Export & Import of Lethal Agents Mediated by TolC
    Rajeev Misra; Fiscal Year: 2006
    ..Besides the obvious role of TolC in antibiotic resistance, its ability to mediate the secretion of a variety of virulence factors in pathogenic bacteria makes it a medically important subject of investigation. ..
  47. PSEUDOMONAS AUTOINDUCER BIOSYNTHESIS AND DRUG DISCOVERY
    Herbert Schweizer; Fiscal Year: 2001
    ..These studies should provide a better understanding of the quorum sensing pathways and lead to the identification of novel anti microbial targets and antimicrobials effective against gram-negative pathogens. ..
  48. STRUCTURE AND ACTION OF STEROIDOGENIC REGULATORY PROTEIN
    HIMANGSHU BOSE; Fiscal Year: 2002
    ..Fulfilling these aims will provide the structural information needed to develop a detailed understanding of how StAR and MLN64 regulate cholesterol movement from the outer to inner mitochondrial membrane. ..
  49. Structures of membrane-bound Bcl-2 apoptotic proteins
    Francesca Marassi; Fiscal Year: 2009
    ....
  50. Non-antiobiotic resistance markers for bacteria
    Herbert Schweizer; Fiscal Year: 2006
    ..pseudomallei, F. tularensis and Y. pestis, as well as other bacterial pathogens for which one faces similar restrictions regarding use of antibiotic selection markers for genetic manipulation. [unreadable] [unreadable] [unreadable]..
  51. Regulation and function of Pseudomonas drug efflux pumps
    Herbert Schweizer; Fiscal Year: 2006
    ..Aim 3: Probe other, perhaps global regulators of efflux operon expression, specifically MexS and PA4878. ..
  52. Role of Chlamydia Pneumoniae in Atherosclerosis
    Cho Chou Kuo; Fiscal Year: 2007
    ..pneumoniae infection and reduction of atherosclerosis and coronary heart disease. ..
  53. Genetic Tools for Pathogenic Bacteria
    Herbert Schweizer; Fiscal Year: 2005
    ....
  54. Study of Mechanisms Leading to Multidrug-Resistant K. pneumoniae and A. baumannii
    John Quale; Fiscal Year: 2008
    ..Using real time reverse transcriptase PCR, expression of genes encoding beta-lactamases, outer membrane porins, and efflux systems will be measured in highly-resistant strains and their expression will be compared to that ..
  55. The mitochondrial permeability transition and heart failure
    William Craigen; Fiscal Year: 2008
    ..It is expected that completion of these goals will identify targets for therapeutic interventions in heart failure. [unreadable] [unreadable] [unreadable]..
  56. Sexually Transmitted Infections & Antimicrobial Peptides
    William Shafer; Fiscal Year: 2009
    ..abstract_text> ..
  57. Vascular tropism of Borrelia burgdorferi
    Jenifer Coburn; Fiscal Year: 2005
    ..The experiments that we are proposing will shed light on the significance of three B. burgdorferi proteins that may facilitate interactions of B. burgdorferi with the vasculature during the natural course of infection in mammals. ..
  58. ATP-DEPENDENT PROTEIN KINASES IN GRAM-POSITIVE BACTERIA
    Milton Saier; Fiscal Year: 2004
    ..abstract_text> ..
  59. Effect of Neisserial Porin in Immune Cell Apoptosis
    Lee Wetzler; Fiscal Year: 2006
    Neisserial porins (gonococcal Protein I [PIA or PIB] or meningococcal class 1 [PorA], 2 or 3 [PorB] proteins) are the major protein constituents of the Neisserial outer membrane...