Genomes and Genes
Summary: Porins are protein molecules that were originally found in the outer membrane of GRAM-NEGATIVE BACTERIA and that form multi-meric channels for the passive DIFFUSION of WATER; IONS; or other small molecules. Porins are present in bacterial CELL WALLS, as well as in plant, fungal, mammalian and other vertebrate CELL MEMBRANES and MITOCHONDRIAL MEMBRANES.
Publications315 found, 100 shown here
- Molecular basis of bacterial outer membrane permeability revisitedHiroshi Nikaido
Department of Molecular and Cell Biology, University of California, Berkeley, California 94720 3202, USA
Microbiol Mol Biol Rev 67:593-656. 2003..With the discovery of protein channels, structural knowledge enables us to understand in molecular detail how porins, specific channels, TonB-linked receptors, and other proteins function...
- Bcl-2 family proteins regulate the release of apoptogenic cytochrome c by the mitochondrial channel VDACS Shimizu
Osaka University Medical School, Biomedical Research Center, Department of Medical Genetics, Suita, Japan
Nature 399:483-7. 1999..Our results indicate that the Bcl-2 family of proteins bind to the VDAC in order to regulate the mitochondrial membrane potential and the release of cytochrome c during apoptosis...
- Adaptive and mutational resistance: role of porins and efflux pumps in drug resistanceLucía Fernández
Centre for Microbial Diseases and Immunity Research, Department of Microbiology and Immunology, University of British Columbia, Vancouver, British Columbia, Canada
Clin Microbiol Rev 25:661-81. 2012..This review evaluates the impact of porins and efflux pumps on two major types of resistance, namely, mutational and adaptive types of resistance, both of ..
- Coding sequence targeting by MicC RNA reveals bacterial mRNA silencing downstream of translational initiationVerena Pfeiffer
Max Planck Institute for Infection Biology, RNA Biology Group, Berlin, Germany
Nat Struct Mol Biol 16:840-6. 2009..The discovery of CDS targeting markedly expands the sequence space for sRNA target predictions in bacteria...
- Outer membrane permeability and antibiotic resistanceAnne H Delcour
Department of Biology, University of Houston, 369 Science and Research Building II, Houston, TX 77204 5001, USA
Biochim Biophys Acta 1794:808-16. 2009..can take through the outer membrane: a lipid-mediated pathway for hydrophobic antibiotics, and general diffusion porins for hydrophilic antibiotics...
- A small non-coding RNA of the invasion gene island (SPI-1) represses outer membrane protein synthesis from the Salmonella core genomeVerena Pfeiffer
Max Planck Institute for Infection Biology, Chariteplatz 1, D 10117 Berlin, Germany
Mol Microbiol 66:1174-91. 2007..This study identifies the first regulatory RNA of an enterobacterial pathogenicity island, and new roles for Hfq and HilD in SPI-1 gene expression...
- Klebsiella pneumoniae outer membrane porins OmpK35 and OmpK36 play roles in both antimicrobial resistance and virulenceYu Kuo Tsai
Division of Infectious Diseases, National Health Research Institutes, Miaoli, Taiwan
Antimicrob Agents Chemother 55:1485-93. 2011OmpK35 and OmpK36 are the major outer membrane porins of Klebsiella pneumoniae. In this study, a virulent clinical isolate was selected to study the role of these two porins in antimicrobial resistance and virulence...
- Porins, efflux pumps and multidrug resistance in Acinetobacter baumanniiJordi Vila
Servei de Microbiologia, Centre de Diagnostic Biomedic, Hospital Clinic, IDIBAPS, Facultat de Medicina, Universitat de Barcelona, Barcelona, Spain
J Antimicrob Chemother 59:1210-5. 2007..acquisition of genetic elements carrying multiple resistant determinants or mutations affecting the expression of porins and/or efflux pump(s), which can affect unrelated antimicrobial agents...
- The porin and the permeating antibiotic: a selective diffusion barrier in Gram-negative bacteriaJean Marie Pages
UMR MD 1, Transporteurs Membranaires, Chimioresistance et Drug Design, Facultés de Médecine et de harmacie, Universite de la Mediterranee, Marseille, 13385, France
Nat Rev Microbiol 6:893-903. 2008..The outer membrane contains various protein channels, called porins, which are involved in the influx of various compounds, including several classes of antibiotics...
- Sus1, a functional component of the SAGA histone acetylase complex and the nuclear pore-associated mRNA export machinerySusana Rodriguez-Navarro
Biochemie Zentrum der Universität Heidelberg BZH, Im Neuenheimer Feld 328, D 69120 Heidelberg, Germany
Cell 116:75-86. 2004..Finally, mRNA export is impaired in sus1 mutants. These data provide an unexpected connection between the SAGA histone acetylase complex and the mRNA export machinery...
- Identification and localization of Myxococcus xanthus porins and lipoproteinsSwapna Bhat
Department of Microbiology, University of Georgia, Athens, Georgia, United States of America
PLoS ONE 6:e27475. 2011..These results suggest that ECM proteins have a unique method of secretion. It is now possible to target lipoproteins to specific IM, OM and ECM locations by manipulating the amino acid sequence near the +1 cysteine processing site...
- Crystal structures of various maltooligosaccharides bound to maltoporin reveal a specific sugar translocation pathwayR Dutzler
Department of Structural Biology, Biozentrum, University of Basel, CH 4056 Basel, Switzerland
Structure 4:127-34. 1996..coli. In particular, it is indispensable for the transport of long maltooligosaccharides, as these do not pass through non-specific porins. An understanding of this intriguing capability requires elucidation of the structural basis.
- Single-virus tracking reveals a spatial receptor-dependent search mechanismEli Rothenberg
Department of Physics, University of Illinois at Urbana Champaign, Urbana, Illinois, USA
Biophys J 100:2875-82. 2011..We discuss the possible implications of our findings for the process of viral receptor-finding in higher systems...
- Complex spatial distribution and dynamics of an abundant Escherichia coli outer membrane protein, LamBKarine A Gibbs
Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA 94305, USA
Mol Microbiol 53:1771-83. 2004..In this study, we have demonstrated that LamB moves along the bacterial surface and that these movements are restricted by an underlying dynamic spiral pattern...
- Vaccination with the Chlamydia trachomatis major outer membrane protein can elicit an immune response as protective as that resulting from inoculation with live bacteriaSukumar Pal
Department of Pathology and Laboratory Medicine, Medical Sciences I, Room D440, University of California, Irvine, Irvine, CA 92697 4800, USA
Infect Immun 73:8153-60. 2005..05). In conclusion, immunization with a purified preparation of the MOMP is as effective as vaccination with viable C. trachomatis in eliciting a protective immune response against a genital challenge in mice...
- Use of OmpU porins for attachment and invasion of Crassostrea gigas immune cells by the oyster pathogen Vibrio splendidusMarylise Duperthuy
Institut Francais de Recherche pour l Exploitation de la Mer, Centre National de la Recherche Scientifique, Universite Montpellier 2, 34095 Montpellier, France
Proc Natl Acad Sci U S A 108:2993-8. 2011OmpU porins are increasingly recognized as key determinants of pathogenic host Vibrio interactions...
- The growth rate of Mycobacterium smegmatis depends on sufficient porin-mediated influx of nutrientsJoachim Stephan
Lehrstuhl fur Mikrobiologie, Friedrich Alexander Universitat Erlangen Nurnberg, Staudtstrasse 5, D 91058 Erlangen, Germany
Mol Microbiol 58:714-30. 2005..Nutrients cross this permeability barrier by diffusion through porins. MspA is the major porin of Mycobacterium smegmatis...
- Nab2p and the Thp1p-Sac3p complex functionally interact at the interface between transcription and mRNA metabolismMercedes Gallardo
Departamento de Genetica, Facultad de Biologia, Universidad de Sevilla, Avenida Reina Mercedes 6, 41012 Sevilla, Spain
J Biol Chem 278:24225-32. 2003..Altogether, our results suggest that Thp1p-Sac3p and Nab2p are functionally related heterogeneous nuclear ribonucleoproteins that define a further link between mRNA metabolism and transcription...
- Structural and dynamical properties of the porins OmpF and OmpC: insights from molecular simulationsAmit Kumar
Istituto Officina dei Materiali CNR UOS SLACS, c o Department of Physics, University of Cagliari, SP Monserrato Sestu Km 0 700, Monserrato I 09042, Italy
J Phys Condens Matter 22:454125. 2010In this paper we investigate the structural and dynamical properties of the two major porins (OmpF and OmpC) in Escherichia coli, using molecular dynamics (MD) simulations...
- Silver-resistant mutants of Escherichia coli display active efflux of Ag+ and are deficient in porinsX Z Li
Department of Microbiology, University of Saskatchewan, Saskatoon, Canada
J Bacteriol 179:6127-32. 1997..outer membrane (OM) permeability to cephalosporins, and all five resistant mutants tested were deficient in major porins, either OmpF or OmpF plus OmpC...
- The murine voltage-dependent anion channel gene family. Conserved structure and functionM J Sampson
Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030, USA
J Biol Chem 272:18966-73. 1997..VDAC3, however, is only able to partially complement the mutant phenotype, suggesting an alternative physiologic function for the VDAC3 protein...
- Complex inhibition of OmpF and OmpC bacterial porins by polyaminesR Iyer
Department of Biology, University of Houston, Houston, Texas 77204 5513, USA
J Biol Chem 272:18595-601. 1997The effects of four polyamines (putrescine, cadaverine, spermidine, and spermine) on the activity of bacterial porins OmpC and OmpF were investigated by electrophysiology...
- Structure of the complex of the colicin E2 R-domain and its BtuB receptor. The outer membrane colicin transloconOnkar Sharma
Department of Biological Sciences, Lilly Hall of Life Sciences, Purdue University, West Lafayette, Indiana 47907, USA
J Biol Chem 282:23163-70. 2007..Arg(452) at the R/C-domain interface in colicin E2 was found have an essential role at a putative site of protease cleavage, which would liberate the C-terminal activity domain for passage through the outer membrane translocon...
- MicC, a second small-RNA regulator of Omp protein expression in Escherichia coliShuo Chen
Ibis Therapeutics, Isis Pharmaceuticals, Inc, Carlsbad, California, USA
J Bacteriol 186:6689-97. 2004..We suggest that the MicF and MicC RNAs act in conjunction with the EnvZ-OmpR two-component system to control the OmpF/OmpC protein ratio in response to a variety of environmental stimuli...
- Role of porins in iron uptake by Mycobacterium smegmatisChristopher M Jones
Department of Microbiology, University of Alabama at Birmingham, 609 Bevill Biomedical Research Building, 845 19th Street South, Birmingham, Alabama 35294, USA
J Bacteriol 192:6411-7. 2010..of iron in Gram-negative bacteria includes diffusion of iron ions or chelates across the outer membrane through porins. The outer membrane of the saprophytic Mycobacterium smegmatis contains the Msp family of porins, which enable the ..
- Sequence polymorphism, predicted secondary structures, and surface-exposed conformational epitopes of Campylobacter major outer membrane proteinQ Zhang
Food Animal Health Research Program, The Ohio State University, Wooster, Ohio 44691, USA
Infect Immun 68:5679-89. 2000..These findings are instrumental in the design of MOMP-based diagnostic tools and vaccines...
- Surface expression, single-channel analysis and membrane topology of recombinant Chlamydia trachomatis Major Outer Membrane ProteinHeather E Findlay
Division of Biomedical Sciences, University of Edinburgh Medical School, George Square, Edinburgh EH8 9XD, UK
BMC Microbiol 5:5. 2005..coli cells, it often misfolds and aggregates. We aimed to improve the surface expression of correctly folded MOMP to investigate the membrane topology of the protein, and provide a system to display native and modified MOMP epitopes...
- High-level carbapenem resistance in a Klebsiella pneumoniae clinical isolate is due to the combination of bla(ACT-1) beta-lactamase production, porin OmpK35/36 insertional inactivation, and down-regulation of the phosphate transport porin phoeFrank M Kaczmarek
Pfizer Global Research and Development, Groton, CT 06340, USA
Antimicrob Agents Chemother 50:3396-406. 2006..Our results with this pair of Klebsiella clinical isolates highlight the complex and evolving nature of multiple drug resistance in this species...
- Caught at its own game: regulatory small RNA inactivated by an inducible transcript mimicking its targetNara Figueroa-Bossi
Centre de Genetique Moleculaire, Centre National de la Recherche Scientifique CNRS, Gif sur Yvette 91198, France
Genes Dev 23:2004-15. 2009..Overall, our findings blur the distinction between effector and target in sRNA regulation, raising the possibility that some of the currently defined targets could actually be inhibitors of sRNA function...
- Site-directed mutagenesis of tyrosine 118 within the central constriction site of the LamB (maltoporin) channel of Escherichia coli. II. Effect on maltose and maltooligosaccharide binding kineticsFrank Orlik
Lehrstuhl fur Biotechnologie, Theodor Boveri Institut Biozentrum der Universität Würzburg, Am Hubland, D 97074 Wurzburg, Germany
Biophys J 83:309-21. 2002..The results suggest that tyrosine 118 has a crucial effect on carbohydrate transport through LamB...
- Review of meningococcal group B vaccinesDan M Granoff
Center for Immunobiology and Vaccine Development, Children s Hospital Oakland Research Institute, Oakland, California, USA
Clin Infect Dis 50:S54-65. 2010..Thus, the prospects have never been better for developing vaccines for prevention of meningococcal disease, including that caused by group B strains...
- A putative alpha-helical porin from Corynebacterium glutamicumKarin Ziegler
Institut fur Organische Chemie und Biochemie, Albert Ludwigs Universitat, Albertstr 21, 79104 Freiburg im Breisgau, Germany
J Mol Biol 379:482-91. 2008..The exchange of material across this barrier requires porins. Porin B (PorB) is one of them...
- Crystal structure of Omp32, the anion-selective porin from Comamonas acidovorans, in complex with a periplasmic peptide at 2.1 A resolutionK Zeth
Max Planck Institut fur Biochemie, Abteilung Molekulare Strukturbiologie, Am Klopferspitz 18a, D 82152, Martinsried, Germany
Structure 8:981-92. 2000b>Porins provide diffusion channels for salts and small organic molecules in the outer membrane of bacteria...
- The yeast nucleoporin Nup53p specifically interacts with Nic96p and is directly involved in nuclear protein importB Fahrenkrog
M E Müller Institute for Structural Biology, Biozentrum, University of Basel, CH 4056 Basel, Switzerland
Mol Biol Cell 11:3885-96. 2000..Whereas Nup53p is directly involved in NLS-mediated protein import by its interaction with the yeast nuclear import receptor Kap95p, it appears not to participate in NES-dependent nuclear export...
- Long-term evolution of antigen repertoires among carried meningococciCaroline O Buckee
Department of Zoology, University of Oxford, Oxford OX1 3PS, UK
Proc Biol Sci 277:1635-41. 2010..We contrast this antigenic structure with the overlapping but relatively stable combinations of the housekeeping genes observed among the same isolates, and use a novel network approach to visualize these relationships...
- The motion of a single molecule, the lambda-receptor, in the bacterial outer membraneLene Oddershede
The Niels Bohr Institute, University of Copenhagen, Blegdamsvej 17, 2100 Copenhagen Ø, Denmark
Biophys J 83:3152-61. 2002..0 x 10(-2) pN/nm to the bacterial membrane. The purpose of the protein motion might be to facilitate transport of maltodextrins through the outer bacterial membrane...
- Structure of the sucrose-specific porin ScrY from Salmonella typhimurium and its complex with sucroseD Forst
Institut fur Biophysik und Strahlenbiologie, Albert Ludwigs Universitat, Freiburg im Breisgau, Germany
Nat Struct Biol 5:37-46. 1998..Two non-overlapping sucrose-binding sites were identified in difference Fourier maps. The higher permeability for sucrose of ScrY as compared to maltoporin is mainly accounted for by differences in their pore-lining residues...
- Implication of porins in beta-lactam resistance of Providencia stuartiiQue Tien Tran
School of Engineering and Science, Jacobs University Bremen, D 28759 Bremen, Germany
J Biol Chem 285:32273-81. 2010..coli cells to β-lactams compared with expression of OmpPst2. All our data enlighten the involvement of porins in the resistance of P. stuartii to β-lactam antibiotics.
- Down-regulation of porins by a small RNA bypasses the essentiality of the regulated intramembrane proteolysis protease RseP in Escherichia coliVéronique Douchin
Signalisation et Réseaux de Régulations Bactériens, Institut de Genetique et Microbiologie, CNRS UMR8621 IFR115, Centre Scientifique d Orsay, Universite Paris Sud, 91405 Orsay Cedex, France
J Biol Chem 281:12253-9. 2006....
- Porins from Salmonella enterica serovar Typhimurium activate the transcription factors activating protein 1 and NF-kappaB through the Raf-1-mitogen-activated protein kinase cascadeMassimiliano Galdiero
Istituto di Microbiologia, Facolta di Medicina e Chirurgia, Seconda Universita degli Studi di Napoli, 80138 Naples, Italy
Infect Immun 70:558-68. 2002In this study we examined the ability of Salmonella enterica serovar Typhimurium porins to activate activating protein 1 (AP-1) and nuclear factor kappaB (NF-kappaB) through the mitogen-activated protein kinase (MAPK) cascade, and we ..
- The role of mitochondrial porins and the permeability transition pore in learning and synaptic plasticityEdwin J Weeber
Division of Neuroscience, Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030, USA
J Biol Chem 277:18891-7. 2002Mitochondrial outer membrane permeability is conferred by a family of porin proteins. Mitochondrial porins conduct small molecules and constitute one component of the permeability transition pore that opens in response to apoptotic ..
- Role of surface-exposed loops of Haemophilus influenzae protein P2 in the mitogen-activated protein kinase cascadeStefania Galdiero
Dipartimento di Chimica Biologica, Università degli Studi di Napoli Federico II and Istituto di Biostrutture e Bioimmagini, CNR, 80134 Naples, Italy
Infect Immun 71:2798-809. 2003The outer membrane of gram-negative bacteria contains several proteins, and some of these proteins, the porins, have numerous biological functions in the interaction with the host; porins are involved in the activation of signal ..
- ATP flux is controlled by a voltage-gated channel from the mitochondrial outer membraneT Rostovtseva
Laboratories of Cell Biology, Department of Zoology, University of Maryland, College Park, Maryland 20742, USA
J Biol Chem 271:28006-8. 1996..Thus, these channels are ideally suited to control ATP flux through the mitochondrial outer membrane and, consequently, mitochondrial function. The block to ATP flow through the closed state is likely to be not steric but electrostatic...
- OMP peptide signals initiate the envelope-stress response by activating DegS protease via relief of inhibition mediated by its PDZ domainNathan P Walsh
Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
Cell 113:61-71. 2003..Escherichia coli respond to misfolded or unfolded outer-membrane porins (OMPs) in the periplasm by inducing sigma(E)-dependent transcription of stress genes in the cytoplasm...
- Single-molecule DNA detection with an engineered MspA protein nanoporeTom Z Butler
Department of Physics, University of Washington, Box 351560, 3910 15th Avenue NE, Seattle, WA 98195, USA
Proc Natl Acad Sci U S A 105:20647-52. 2008..Our results introduce MspA as a nanopore for nucleic acid analysis and highlight its potential as an engineerable platform for single-molecule detection and characterization applications...
- Mobility of BtuB and OmpF in the Escherichia coli outer membrane: implications for dynamic formation of a translocon complexJeff Spector
Department of Physics, Purdue University, West Lafayette, Indiana, USA
Biophys J 99:3880-6. 2010..Thus, formation of the outer membrane translocon for cellular import of the nuclease colicins is a demonstrably dynamic process, because it depends on lateral diffusion of BtuB and collisional interaction with relatively immobile OmpF...
- Maintaining a healthy SPANC balance through regulatory and mutational adaptationThomas Ferenci
School of Molecular and Microbial Biosciences, The University of Sydney, NSW 2006, Australia
Mol Microbiol 57:1-8. 2005..selective pressures working in opposite directions (antagonistic pleiotropy) result in polymorphisms affecting porins and RpoS...
- Recognition of heptameric seed sequence underlies multi-target regulation by RybB small RNA in Salmonella entericaRoberto Balbontin
Departamento de Genetica, Facultad de Biologia, Universidad de Sevilla, 41080 Seville, Spain
Mol Microbiol 78:380-94. 2010..analysis to dissect the functional anatomy of RybB, a σ(E)-dependent sRNA that regulates the synthesis of major porins in Escherichia coli and Salmonella...
- Cutting edge: Immune stimulation by neisserial porins is toll-like receptor 2 and MyD88 dependentPaola Massari
Department of Medicine, Division of Infectious Diseases, Boston University School of Medicine, Boston, MA 02118, USA
J Immunol 168:1533-7. 2002The immunopotentiating activity of neisserial porins, the major outer membrane protein of the pathogenic Neisseria, is mediated by its ability to stimulate B cells and up-regulate the surface expression of B7-2...
- The VR2 epitope on the PorA P1.7-2,4 protein is the major target for the immune response elicited by the strain-specific group B meningococcal vaccine MeNZBD R Martin
Insitute of Environmental Science and Research ESR, Porirua, New Zealand
Clin Vaccine Immunol 13:486-91. 2006..4 epitope of the PorA protein supports the public health decision to use a strain-specific vaccine for the control of New Zealand's epidemic of meningococcal disease...
- Structure and function of bacterial outer membrane proteins: barrels in a nutshellR Koebnik
Biozentrum Basel, Abteilung Mikrobiologie, Klingelbergstr 50, CH 4056 Basel, Switzerland
Mol Microbiol 37:239-53. 2000..They include the OmpA membrane domain, the OmpX protein, phospholipase A, general porins (OmpF, PhoE), substrate-specific porins (LamB, ScrY) and the TonB-dependent iron siderophore transporters FhuA and ..
- Molecular simulations reveal the mechanism and the determinants for ampicillin translocation through OmpFAmit Kumar
Department of Physics, University of Cagliari and Istituto Officina dei Materiali CNR UOS SLACS, I 09042 Monserrato Ca, Italy
J Phys Chem B 114:9608-16. 2010..As a whole, our analysis opens the way to chemical modifications of antibiotics to allow improving uptake through porins contributing to combat bacterial resistance.
- Differential expression of the OmpF and OmpC porin proteins in Escherichia coli K-12 depends upon the level of active OmpRC Y Lan
Division of Biological Sciences, University of California, Davis, 95616, USA
J Bacteriol 180:171-4. 1998..This result supports a key prediction of the current model of porin regulation, which states that the differential regulation of OmpF and OmpC is a direct consequence of the cellular level of the active form of OmpR...
- Transcription regulation of ompF and ompC by a single transcription factor, OmpRTakeshi Yoshida
Department of Biochemistry, Robert Wood Johnson Medical School, Piscataway, New Jersey 08854, USA
J Biol Chem 281:17114-23. 2006....
- Role of porins for uptake of antibiotics by Mycobacterium smegmatisOlga Danilchanka
Department of Microbiology, University of Alabama at Birmingham, Alabama 35294, USA
Antimicrob Agents Chemother 52:3127-34. 2008..Deletion of the porins MspA and MspC drastically increased the resistance of M...
- Roles of beta-lactamases and porins in activities of carbapenems and cephalosporins against Klebsiella pneumoniaeL Martinez-Martinez
Department of Microbiology, School of Medicine, University of Seville, Seville, Spain
Antimicrob Agents Chemother 43:1669-73. 1999..provided resistance to imipenem (up to 64 microg/ml) and meropenem (up to 16 microg/ml) in strains deficient in porins. Carbapenem resistance showed little inoculum effect, was not affected by clavulanate but was blocked by BRL 42715,..
- The voltage-dependent anion channel is a receptor for plasminogen kringle 5 on human endothelial cellsMario Gonzalez-Gronow
Department of Pathology, Duke University Medical Center, Durham, North Carolina 27710, USA
J Biol Chem 278:27312-8. 2003..K5 binding to endothelial cells also induces a decrease in intracellular pH and hyperpolarization of the mitochondrial membrane. These studies suggest that VDAC is a receptor for K5...
- Structural basis for sugar translocation through maltoporin channels at 3.1 A resolutionT Schirmer
Department of Structural Biology, University of Basel, Switzerland
Science 267:512-4. 1995....
- MspA provides the main hydrophilic pathway through the cell wall of Mycobacterium smegmatisC Stahl
Lehrstuhl fur Mikrobiologie, Friedrich Alexander Universitat Erlangen Nurnberg, Staudtstr 5, 91058 Erlangen, Germany
Mol Microbiol 40:451-64. 2001..smegmatis and was only partially replaced by fewer porins in the cell wall of the DeltamspA mutant [corrected] This is the first experimental evidence that porins are the ..
- Emerging issues in the management of infections caused by multidrug-resistant gram-negative bacteriaLouis B Rice
Louis Stokes Cleveland VA Medical Center and Case Western Reserve University School of Medicine, Cleveland, OH 44106, USA
Cleve Clin J Med 74:S12-20. 2007....
- Essential role of voltage-dependent anion channel in various forms of apoptosis in mammalian cellsS Shimizu
Osaka University Graduate School of Medicine, Biomedical Research Center, Department of Medical Genetics, Osaka 565-0871, Japan
J Cell Biol 152:237-50. 2001..Taken together, our data provide evidence that the VDAC plays an essential role in apoptogenic cytochrome c release and apoptosis in mammalian cells...
- Genetic diversity and mosaicism at the por locus of Neisseria gonorrhoeaeT C Fudyk
Department of Medical Microbiology, University of Manitoba, Winnipeg, Manitoba, Canada
J Bacteriol 181:5591-9. 1999..Despite extensive sequence variability, por expressed by gonococcal isolates of different geographic origin exhibited conserved patterns of nucleotide change, suggesting that diversity among por alleles may also be finite...
- Immunogenic characterization of outer membrane porins OmpC and OmpF of porcine extraintestinal pathogenic Escherichia coliCanying Liu
State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China
FEMS Microbiol Lett 337:104-11. 2012..In this study, two outer membrane porins, OmpC and OmpF, of porcine ExPEC were cloned and expressed to investigate their immunogenicity...
- Sequence variation in the porA gene of a clone of Neisseria meningitidis during epidemic spreadJ Jelfs
WHO Collaborating Centre for Reference and Research on Meningococci, Department of Bacteriology, National Institute of Public Health, N 0403 Oslo, Norway
Clin Diagn Lab Immunol 7:390-5. 2000..The magnitude of microevolutionary mechanisms available in meningococci and the remarkable genetic flexibility of these bacteria need to be considered in relation to PorA vaccine development...
- Functional comparison of the binding of factor H short consensus repeat 6 (SCR 6) to factor H binding protein from Neisseria meningitidis and the binding of factor H SCR 18 to 20 to Neisseria gonorrhoeae porinJutamas Shaughnessy
Division of Infectious Diseases and Immunology, University of Massachusetts Medical School, Lazare Research Building, Room 370I, Plantation Street, Worcester, MA 01605, USA
Infect Immun 77:2094-103. 2009..Differences in fH binding to these two related pathogens may be important for modulating their individual responses to host immune attack...
- Amino acids important for DNA recognition by the response regulator OmpRJee Eun Rhee
Department of Microbiology and Immunology, University of Illinois, Chicago, IL 60612, USA
J Biol Chem 283:8664-77. 2008..The flexible linker of OmpR enables the second monomer to bind in multiple orientations (head-to-tail and head-to-head), depending on the specific DNA contacts...
- The structure of BtuB with bound colicin E3 R-domain implies a transloconGenji Kurisu
Department of Biological Sciences, Purdue University, Lilly Hall of Life Sciences, 915 W State St, West Lafayette, Indiana 47907 1392, USA
Nat Struct Biol 10:948-54. 2003..The translocator was concluded to be OmpF from the occlusion of OmpF channels by colicin E3...
- Defining targets for complement components C4b and C3b on the pathogenic neisseriaeLisa A Lewis
Division of Infectious Diseases and Immunology, University of Massachusetts Medical School, Lazare Research Building, Room 370I, 364 Plantation Street, Worcester, MA 01605, USA
Infect Immun 76:339-50. 2008..The binding of C3b and C4b to Opa provides a rationale for the recovery of predominantly "transparent" (Opa-negative) neisserial isolates from persons with invasive disease, where the bacteria encounter high levels of complement...
- Chlamydia trachomatis infection: incidence, health costs and prospects for vaccine developmentK W Beagley
Discipline of Immunology and Microbiology, Faculty of Medicine and Health Sciences, University of Newcastle, NSW 2308, Newcastle, Australia
J Reprod Immunol 48:47-68. 2000..Thus there are many challenges for the development of a successful vaccine which must elicit immunity against multiple serovars while at the same time minimising damaging pro-inflammatory immune responses...
- Understanding ion conductance on a molecular level: an all-atom modeling of the bacterial porin OmpFSoroosh Pezeshki
School of Engineering and Science, Jacobs University Bremen, Bremen, Germany
Biophys J 97:1898-906. 2009..Reduction of the negatively charged residues decreases the conductance by half, whereas cationic residues enhance the conductance. Experiments on mutants confirm the results of the molecular-level simulations...
- VDAC regulation by the Bcl-2 family of proteinsY Tsujimoto
Department of Medical Genetics, Biomedical Research Center, Osaka University Graduate School of Medicine, CREST of Japan Science and Technology Corp JST, Suita, Osaka, Japan
Cell Death Differ 7:1174-81. 2000..Cell Death and Differentiation (2000) 7, 1174 - 1181..
- Protection against an intranasal challenge by vaccines formulated with native and recombinant preparations of the Chlamydia trachomatis major outer membrane proteinGuifeng Sun
Department of Pathology and Laboratory Medicine, Medical Sciences, Room D440, University of California, Irvine, CA 92697 4800, United States
Vaccine 27:5020-5. 2009..05). In conclusion, vaccination with a preparation of the nMOMP elicited a more robust protection than immunization with rMOMP, suggesting that the conformational structure of MOMP is critical for inducing strong protection...
- Serum bactericidal activity and isotype distribution of antibodies in toddlers and schoolchildren after vaccination with RIVM hexavalent PorA vesicle vaccineE de Kleijn
Department of Pediatrics, Sophia Children's Hospital/University Hospital, Rotterdam, The Netherlands
Vaccine 20:352-8. 2001..The isotype distribution of the response is not age-dependent...
- VrrA mediates Hfq-dependent regulation of OmpT synthesis in Vibrio choleraeTianyan Song
Department of Molecular Biology, The Laboratory for Molecular Infection Medicine Sweden, Umeå Center for Microbial Research, Umea University, SE 901 87 Umea, Sweden
J Mol Biol 400:682-8. 2010..In addition to pH/temperature signals via the ToxR regulon and carbon source signals via the cAMP-CRP complex, OmpT is further regulated by signals received via the sigma(E) regulon through VrrA...
- Identification and sequence analysis of Treponema pallidum tprJ, a member of a polymorphic multigene familyL V Stamm
Department of Epidemiology, School of Public Health, University of North Carolina, Chapel Hill 27599 7400, USA
FEMS Microbiol Lett 169:155-63. 1998..The deduced amino acid sequence of T. pertenue TprJ is 87.3% identical to that of T. pallidum TprJ. This is the first report of significant sequence differences within homologous genes of T. pallidum and T. pertenue...
- Directed epitope delivery across the Escherichia coli outer membrane through the porin OmpFNicholas G Housden
Department of Biology, Area 10, and York Structural Biology Laboratory, University of York, Heslington, York YO10 5DD, United Kingdom
Proc Natl Acad Sci U S A 107:21412-7. 2010The porins OmpF and OmpC are trimeric Î²-barrel proteins with narrow channels running through each monomer that exclude molecules > 600 Da while mediating the passive diffusion of small nutrients and metabolites across the Gram-..
- Computational electrophysiology: the molecular dynamics of ion channel permeation and selectivity in atomistic detailCarsten Kutzner
Department of Theoretical and Computational Biophysics, Max Planck Institute for Biophysical Chemistry, Gottingen, Germany
Biophys J 101:809-17. 2011..We show that our method accurately predicts ion conductance and selectivity and elucidates ion conduction mechanisms in great detail. Handles for overcoming channel-related antibiotic resistance are identified...
- Site-directed mutagenesis of the greasy slide aromatic residues within the LamB (maltoporin) channel of Escherichia coli: effect on ion and maltopentaose transportKatrin Denker
Lehrstuhl fur Biotechnologie, Theodor Boveri Institut Biozentrum der Universität Würzburg, Am Hubland, D 97074 Wurzburg, Germany
J Mol Biol 352:534-50. 2005..For most mutants, k1 decreased and k(-1) increased. The possible influence of the aromatic residues of the greasy slide on carbohydrate and ion transport through LamB is discussed...
- Crystal structures explain functional properties of two E. coli porinsS W Cowan
Department of Structural Biology, University of Basel, Switzerland
Nature 358:727-33. 1992b>Porins form aqueous channels that aid the diffusion of small hydrophilic molecules across the outer membrane of Gram-negative bacteria...
- Serum immune responses predict rapid disease progression among children with Crohn's disease: immune responses predict disease progressionMarla C Dubinsky
Department of Pediatrics, Genetics Institute, Cedars Sinai Medical Center, Los Angeles, California, USA
Am J Gastroenterol 101:360-7. 2006..We examined the association of immune responses to microbial antigens with disease behavior and prospectively determined the influence of immune reactivity on disease progression in pediatric CD patients...
- Preclinical safety and immunogenicity evaluation of a nonavalent PorA native outer membrane vesicle vaccine against serogroup B meningococcal diseasePatricia Kaaijk
Unit Vaccinology, Centre for Infectious Disease Control CIb, National Institute for Public Health and the Environment RIVM, Bilthoven, The Netherlands
Vaccine 31:1065-71. 2013..In the present study, the safety and immunogenicity of this next-generation NonaMen vaccine were evaluated following repeated vaccination in rabbits and mice...
- Structural basis for solute transport, nucleotide regulation, and immunological recognition of Neisseria meningitidis PorBMikio Tanabe
Department of Pharmacology, Vanderbilt University Medical Center, Nashville, TN 37232 6600, USA
Proc Natl Acad Sci U S A 107:6811-6. 2010..meningitidis and can be recognized by receptors of the host innate immune system. Features of PorB suggest that Toll-like receptor mediated recognition outer membrane proteins may be initiated with a nonspecific electrostatic attraction...
- Mucosal immunization with recombinant MOMP genetically linked with modified cholera toxin confers protection against Chlamydia trachomatis infectionShree R Singh
Department of Mathematics and Science, Alabama State University, Montgomery, AL 36101, USA
Vaccine 24:1213-24. 2006..n. immunization of mice with rMOMP. This is a first report using genetic fusion of cholera toxin and MOMP genes and provides a novel approach for the design and development of a mucosal vaccine against Chlamydia...
- Additive and synergistic bactericidal activity of antibodies directed against minor outer membrane proteins of Neisseria meningitidisVincent E Weynants
GlaxoSmithKline Biologicals, Rue de l Institut 89, B 1330 Rixensart, Belgium
Infect Immun 75:5434-42. 2007..With minor OMPs, this threshold is reached when more than one antigen is targeted, and this allows cross-protection...
- Proteomic analysis of human U937 cell line activation mediated by Haemophilus influenzae type b P2 porin and its surface-exposed loop 7Valeria Severino
Department of Life Science, Second University of Naples, Via Vivaldi 43, I 81100 Caserta, Italy
J Proteome Res 9:1050-62. 2010..P2 porin, one of the best-characterized porins in terms of its functional characteristics, is the most abundant OMP in Hib and has also been shown to possess ..
- Stability of PorA during a meningococcal disease epidemicA F Devoy
Communicable Disease Group, Institute of Environmental Science and Research, Porirua, New Zealand
J Clin Microbiol 43:832-7. 2005..7-2,4 PorA protein of meningococci responsible for New Zealand's epidemic has remained relatively stable over 13 years and support the use of a strain-specific outer membrane vesicle vaccine to control the epidemic...
- A tetrameric porin limits the cell wall permeability of Mycobacterium smegmatisHarald Engelhardt
Max Planck Institut fur Biochemie, Abteilung Molekulare Strukturbiologie, Am Klopferspitz 18a, D 82152 Martinsried, Germany
J Biol Chem 277:37567-72. 2002..Thus, MspA is drastically different from the trimeric porins of Gram-negative bacteria and represents a new class of channel proteins...
- Insights into pilus assembly and secretion from the structure and functional characterization of usher PapCYihua Huang
Howard Hughes Medical Institute and Department of Biochemistry, University of Texas Southwestern Medical Center, 6001 Forest Park Road, Dallas, TX 75390, USA
Proc Natl Acad Sci U S A 106:7403-7. 2009..The plug domain has a dual function: gating the beta-pore and participating in pilus assembly...
- Specificity of the glucose channel formed by protein D1 of Pseudomonas aeruginosaJ Trias
Department of Microbiology and Immunology, University of California, Berkeley 94720
Biochim Biophys Acta 938:493-6. 1988..This finding and the permeation rates of various glucose analogs suggest that, although the channel has a specific recognition site for glucose, it functions in a manner very different from that of the glucose carrier of erythrocytes...
- Structure of maltoporin from Salmonella typhimurium ligated with a nitrophenyl-maltotriosideJ E Meyer
Institut für Organische Chemie und Biochemie Albert Ludwigs Universität, Freiburg im Breisgau Germany
J Mol Biol 266:761-75. 1997..with the structure of the homologous porin from Escherichia coli as well as with the sequences of other related porins showed that there are regions of appreciable sequence and structure variability, despite close overall similarity...
- Maltodextrin transport through lambAlain Charbit
Laboratoire de Microbiologie, Inserm U 570, Faculte de Medecine Necker, 156, rue de Vaugirard, 75730 Paris Cedex 15, France
Front Biosci 8:s265-74. 2003..Genetic and biochemical analyses suggest that some of the surface loops participate directly in the orientation and entry of maltooligosaccharides into the channel and, thus, control access to the binding site...
- Genetic study of a membrane protein: DNA sequence alterations due to 17 lamB point mutations affecting adsorption of phage lambdaJ M Clement
Unite de Programmation Moleculaire et Toxicologie Genetique, CNRS LA 271, INSERM U 163, Institut Pasteur, Paris, France
EMBO J 2:77-80. 1983..Implications of these results for the topological organization of the lambda receptor as well as possible reasons for the limited number of altered sites detected are discussed...
- Substrate-specific diffusion of select dicarboxylates through Chlamydia trachomatis PorBA Kubo
Division of Infectious Diseases, School of Public Health, 140 Earl Warren Hall, University of California, Berkeley, CA 94720, USA
Microbiology 147:3135-40. 2001Chlamydiae contain two porins, MOMP and PorB, that facilitate diffusion of solutes through the outer membrane. MOMP is a general porin that permits the diffusion of a wide variety of compounds including carbohydrates and amino acids...
- Porins in prokaryotes and eukaryotes: common themes and variationsKornelius Zeth
Max Planck Institute of Developmental Biology, Department Protein Evolution, Spemannstr 35, D 72076 Tubingen, Germany
Biochem J 431:13-22. 2010..Despite the lack of a clear evolutionary link between these protein families, their common principles and differences in assembly, architecture and function are summarized in the present review...
- Antibiotic translocation through membrane channels: temperature-dependent ion current fluctuation for catching the fast eventsKozhinjampara R Mahendran
School of Engineering and Science, Jacobs University Bremen, Campus Ring 1, Bremen, Germany
Eur Biophys J 38:1141-5. 2009..The activation energy for ampicillin translocation through OmpF is estimated to be around 13 kT. Temperature-dependent study gives new insights into the faster translocation of small substrates through biological nanopores...
- Crystal structures of the OmpF porin: function in a colicin transloconEiki Yamashita
Department of Biological Sciences, Purdue University, West Lafayette, IN 47907 1392, USA
EMBO J 27:2171-80. 2008..Involvement of OmpF in colicin passage through the OM was further documented by immuno-extraction of an OM complex, the colicin translocon, consisting of colicin E3, BtuB and OmpF...
- Mutagenesis and functional reconstitution of chlamydial major outer membrane proteins: VS4 domains are not required for pore formation but modify channel functionE S Hughes
Department of Biomedical Sciences, University of Edinburgh, Edinburgh EH8 9XD, United Kingdom
Infect Immun 69:1671-8. 2001..If MOMPs adopt a beta-barrel fold, like bacterial porins, the VS domains may form extramembranous loops and the conserved regions of the protein may correspond to ..
- Vibrio cholerae OmpU and OmpT porins are differentially affected by bileJamie A Wibbenmeyer
Department of Biology and Biochemistry, University of Houston, Houston, Texas 77204 5001, USA
Infect Immun 70:121-6. 2002..In order to further understand the role of the OmpT and OmpU porins in the ability of V...
- Evidence for secretory pathway localization of a voltage-dependent anion channel isoformR Buettner
Institute of Pathology, University Hospital Rheinisch Westfälische Technische Hochschule Aachen, Pauwelstrasse 30, D 52074 Aachen, Germany
Proc Natl Acad Sci U S A 97:3201-6. 2000Voltage-dependent anion channels (VDACs) are pore-forming proteins (porins) that form the major pathway for movement of adenine nucleotides through the outer mitochondrial membrane...
- OmpK26, a novel porin associated with carbapenem resistance in Klebsiella pneumoniaeLaura García-Sureda
Instituto Universitario de Investigaciones en Ciencias de la Salud, Universidad de las Islas Baleares, Palma de Mallorca, Spain
Antimicrob Agents Chemother 55:4742-7. 2011..Loss of the expression of the major nonspecific porins OmpK35/36 is a frequent feature in these isolates...
- Characterization and targeted disruption of murine Nup50, a p27(Kip1)-interacting component of the nuclear pore complexM Smitherman
Clinical Research, Fred Hutchinson Cancer Research Center, Seattle, Washington, 98109, USA
Mol Cell Biol 20:5631-42. 2000..We conclude that Nup50 is a nucleoporin with essential functions during mouse development...
- Allosteric regulation of the sensory protease DegSRANDALL VERNON MAULDIN; Fiscal Year: 2011..Under heat-shock conditions, outer membrane porins (OMP) misfold and accumulate in the periplasm...
- Structural and biochemical characterization of the OprD membrane protein familyBert van den Berg; Fiscal Year: 2011..In most gram-negative bacteria such as E. coli, the majority of small molecules are taken up by porins, such as OmpF and OmpC. Porins are non-specific channels and do not bind their substrates...
- Computational Assembly of Beta Barrel Membrane ProteinJie Liang; Fiscal Year: 2013..3) Develop engineering principles for designing ?-barrel porins with desirable stability, oligomerization state, and pore geometry...
- The interaction between outer membrane porins and toll-like receptorsT M Iverson; Fiscal Year: 2011..One set of TLR ligands, the Outer Membrane Proteins (OMPs), or porins, are transmembrane 2-barrel proteins...
- Neisserial Porins and Antigen Presenting CellsLEE MARK WETZLER; Fiscal Year: 2013..Moreover, we shall further examine the mechanism of PorB adjuvant activity via the use of MyD88 floxed mice bred with mice expressing cell specific cre recombinase. ..
- BIOCHEMISTRY OF BACTERIAL CELL MEMBRANESHiroshi Nikaido; Fiscal Year: 2013..membrane of Pseudomonas aeruginosa or Acinetobacter spp, with their exceptionally low-permeability channels or porins. The molecular mechanisms that make the channel protein to show such a low permeability appear to be in the ..
- HTS for Cytochrome C Synthesis PathwaysRobert G Kranz; Fiscal Year: 2011..has recently shown that large molecule, heme analogs inhibit specific steps, but these analogs require specific porins for access...
- TRP channels in supramolecular complexes with Inorganic Polyphosphate and PolyhydEleonora Zakharian; Fiscal Year: 2013..polyP/PHB complexes have been found in association with a number of membrane proteins, including ion channels and porins. Moreover, these complexes were shown to be important for normal functioning of their hosts...
- Markers predicting response to therapy for KPC-producing Klebsiella pneumoniaeYohei Doi; Fiscal Year: 2013..these agents (lipid A modifications for colistin, and the expression levels of blaKPC and ompK35/36 encoding major porins for doripenem)...
- STRUCTURES OF SIDEROPHORE MEMBRANE TRANSPORT PROTEINSDick van der Helm; Fiscal Year: 2000..The complexes are too large (M.W. 800-1500) to be transported by porins. For competitive reasons microorganisms produce often more than one siderophore, and the structures of ..
- Porins of Mycobacterium tuberculosisMichael Niederweis; Fiscal Year: 2009..tuberculosis by water-filled channel proteins. These porins are the key proteins for uptake of hydrophilic solutes in mycobacteria. We recently discovered two porins of M...
- STRUCTURE OF BACTERIAL MEMBRANE PROTEINSR Garavito; Fiscal Year: 1992The crystallization of transmembrane channel-forming proteins (porins) from the outer membrane of enteric (gram negative) bacteria (e.g., E...
- The OppA PEPTIDE Permease HOMOLOGUE OF ORAL SPIROCHETESJ Fenno; Fiscal Year: 2001..complements studies underway in this laboratory on assembly of membrane complexes comprised of outer membrane porins and proteases, and includes novel approaches to the study of the role of T. denticola in periodontal pathogenesis...
- OUTER MEMBRANE PERMEABILITY OF PSEUDOMONAS CEPACIAJane Burns; Fiscal Year: 1992..To examine these hypotheses, this proposal will characterize porins from isogenic permeable and impermeable strains functionally, biochemically and genetically...
- HOST CELL RECOGNITION OF SALMONELLA TYPHIMURIUMTHOMAS PISTOLE; Fiscal Year: 2003..The first objective is to determine whether structures found on the outer surface of Salmonella, known as porins, are involved in the recognition of this pathogen by human neutrophils...
- SURFACE PROTEINS OF NEISSERIA GONORRHOEAEMilan Blake; Fiscal Year: 1991..Several different antigens are potentially good vaccine candidates such as pili and porins. However, there are others that might potentially be hazardous such as protein 111 and the lipooligosaccharides...
- A Genome-Derived, Epitope-Driven H. Pylori VaccineAnne De Groot; Fiscal Year: 2006..Epitopes derived from antigens previously shown in vivo as protective (e.g., enzymes, porins, adhesins, heat shock proteins), as well as novel epitopes from proteins not previously implicated in protection ..
- Environmental Modulation of ToxT-dependent TranscriptionKARL KLOSE; Fiscal Year: 2007..These studies include: (i). determination of the effect of the porins OmpU and OmpT (which are known to be differentially permeable to bile) on bile modulation of ToxT activity, (ii)...
- QUADRUPOLE TIME OF FLIGHT MASS SPECTROMETERGary Nelsestuen; Fiscal Year: 2001..b>Porins of pathogenic E...
- STRUCTURE-FUNCTION STUDIES OF MOLECULAR CHAPERONESDavid McKay; Fiscal Year: 2007..In gram negative bacteria, specific periplasmic chaperones facilitate correct maturation of outer membrane porins. Using recent information on the structure and peptide binding specificity of one of these chaperones, E...
- 3D POISSON-NERNST-PLANCK THEORYRob Coalson; Fiscal Year: 2002..equations, and, especially, to apply the method to a variety of biological systems including gramicidin, several porins, and the nicotinic acetylcholine receptor...
- IMMUNOPROTECTIVE POTENTIAL OF PORINS AND ANTI-PORIN MABSShiva Singh; Fiscal Year: 1991..abstract): This proposal deals with the study of the immunoprotective potential of Salmonella typhimurium porins and anti-porin monoclonal antibodies in experimental murine salmonellosis...
- Zn(II) metallochaperones in E. coliMichael Crowder; Fiscal Year: 2007..putative, soluble Zn(ll)-metallochaperones that are thought to deliver Zn(ll) from outer membrane-bound channels/porins to the ribosomes and/or proteins. We propose to utilize the following strategy to achieve our goals...
- What controls the thickness of biological membranesJonathan Sachs; Fiscal Year: 2006..OmpF trimeric porins from E...
- 2008 Bacterial Cell Surfaces Gordon Research ConferenceANNE DELCOUR; Fiscal Year: 2008..Success in these research endeavors is enhanced by the exchange of information and ideas promoted by the Bacterial Cell Surfaces Gordon Conference. [unreadable] [unreadable] [unreadable]..
- MOLECULAR PROPERTIES AND MODULATION OF BACTERIAL PORINSANNE DELCOUR; Fiscal Year: 2003b>Porins are large channels of the outer membrane of Gram-negative bacteria, which represent the major entry pathway for hydrophilic solutes into these organisms...
- Genetic screens to identify mycobacterial porinsMartin Pavelka; Fiscal Year: 2008..To facilitate uptake of hydrophilic solutes, mycobacteria, like Gram-negative organisms, utilize porins which are water-filled channels that allow passage of hydrophilic compounds such as nutrients and antibiotics ..
- Ligand-gated Transport through FepAPHILLIP KLEBBA; Fiscal Year: 2009..We will use fluorescence methods to study these questions. ..
- Ehrilchiacidal Mechanisms In LeukocytesYasuko Rikihisa; Fiscal Year: 2009..The results may point to a potential target for new treatment and prevention of HME and HGE. ..
- GENE EXPRESSION BY HUMAN GRANULOCYTIC EHRLICHIOSIS AGYasuko Rikihisa; Fiscal Year: 2004..These efforts are expected to reveal an important role for the p44-multigene family in strategies of adaptive surface variation and avoidance of host immune responses. ..
- Molecular basis meningococcal group A capsular immunityDan Granoff; Fiscal Year: 2007..abstract_text> ..
- Neisseria meningitidis antigens expressed in infectionDan Granoff; Fiscal Year: 2005..meningitidis disease, including group B strains for which there is currently no vaccine available. Also, characterization of gene expression in an in vivo model will lead to a better understanding of meningococcal pathogenesis. ..
- PATHOGENESIS OF CHLAMYDIAL OCULAR INFECTIONSDeborah Dean; Fiscal Year: 2004..A greater understanding of the pathogenesis of trachoma will aid in developing public health interventions and a vaccine that can be implemented in trachoma endemic areas throughout the world. ..
- Comparison of Human Ehrlichiosis Agent GenomesYasuko Rikihisa; Fiscal Year: 2010..chaffeensis virulence determinants and their pathogenic mechanisms. The results may point to potential chemotherapy, chemopreventive and/or vaccine candidates for treatment and prevention of human ehrlichiosis. ..
- Hydrophobics transport across the outer membraneBert van den Berg; Fiscal Year: 2009..These structures will also allow us to determine whether mechanistic differences exist between LCFA and xenobiotics transport. ..
- Folding and design of beta sheets in membranesWilliam C Wimley; Fiscal Year: 2010..This information will be used to create a public database of potential outer membrane proteins ..
- C. trachomatis genomics, strain typing, and evolutionDeborah Dean; Fiscal Year: 2008..unreadable] [unreadable]..
- STRUCTURE BASED DRUG DESIGNRobert Stroud; Fiscal Year: 2004..Public domain access to the structures of therapeutic targets and technology will encourage their use by major pharmaceutical companies for the better design of therapeutics. ..
- SIGNAL SEQUENCE RECOGNITION AND PROTEIN TARGETINGRobert Stroud; Fiscal Year: 2007..These will instruct in the mechanism of replacing the role of RNA by protein, and therefore in the essence of the dynamic roles of SRP RNA and cpSRP43. ..
- THYMIDYLATE SYNTHASERobert Stroud; Fiscal Year: 2001..Irwin Kunts, UCSF. Some mechanistic question will be approached using NMR spectroscopy in collaboration with Dr. Thomas James, UCSF. ..
- Molecular Basis for Transmembrane Conduction & SignalingRobert Stroud; Fiscal Year: 2006..A further aim is to improve crystals of the Acetylcholine receptor as a means of three-dimensional structure determination of an archetype of gating mechanisms in an archetype of neuroreceptor superfamilies. ..
- Pathogenesis of Persistent Chlamydial STDsDeborah Dean; Fiscal Year: 2007..1 Description, ..
- Plasmid-Mediated Quinolone ResistanceGeorge Jacoby; Fiscal Year: 2007..unreadable] [unreadable]..
- ROLES OF EHRLICHIAL OUTER MEMBRANE PROTEIN IN PARASITISMYasuko Rikihisa; Fiscal Year: 2001..Aim 5 addresses hypothesis 3 by examining the effects of immunization with the ehrlichial OMP in a mouse model of human ehrlichiosis. ..
- 2005 Mechanisms of Membrane Transport Gordon ConferenceRobert Stroud; Fiscal Year: 2005..abstract_text> ..
- Export & Import of Lethal Agents Mediated by TolCRajeev Misra; Fiscal Year: 2006..Besides the obvious role of TolC in antibiotic resistance, its ability to mediate the secretion of a variety of virulence factors in pathogenic bacteria makes it a medically important subject of investigation. ..
- PSEUDOMONAS AUTOINDUCER BIOSYNTHESIS AND DRUG DISCOVERYHerbert Schweizer; Fiscal Year: 2001..These studies should provide a better understanding of the quorum sensing pathways and lead to the identification of novel anti microbial targets and antimicrobials effective against gram-negative pathogens. ..
- STRUCTURE AND ACTION OF STEROIDOGENIC REGULATORY PROTEINHIMANGSHU BOSE; Fiscal Year: 2002..Fulfilling these aims will provide the structural information needed to develop a detailed understanding of how StAR and MLN64 regulate cholesterol movement from the outer to inner mitochondrial membrane. ..
- Structures of membrane-bound Bcl-2 apoptotic proteinsFrancesca Marassi; Fiscal Year: 2009....
- Non-antiobiotic resistance markers for bacteriaHerbert Schweizer; Fiscal Year: 2006..pseudomallei, F. tularensis and Y. pestis, as well as other bacterial pathogens for which one faces similar restrictions regarding use of antibiotic selection markers for genetic manipulation. [unreadable] [unreadable] [unreadable]..
- Regulation and function of Pseudomonas drug efflux pumpsHerbert Schweizer; Fiscal Year: 2006..Aim 3: Probe other, perhaps global regulators of efflux operon expression, specifically MexS and PA4878. ..
- Role of Chlamydia Pneumoniae in AtherosclerosisCho Chou Kuo; Fiscal Year: 2007..pneumoniae infection and reduction of atherosclerosis and coronary heart disease. ..
- Genetic Tools for Pathogenic BacteriaHerbert Schweizer; Fiscal Year: 2005....
- Study of Mechanisms Leading to Multidrug-Resistant K. pneumoniae and A. baumanniiJohn Quale; Fiscal Year: 2008..Using real time reverse transcriptase PCR, expression of genes encoding beta-lactamases, outer membrane porins, and efflux systems will be measured in highly-resistant strains and their expression will be compared to that ..
- The mitochondrial permeability transition and heart failureWilliam Craigen; Fiscal Year: 2008..It is expected that completion of these goals will identify targets for therapeutic interventions in heart failure. [unreadable] [unreadable] [unreadable]..
- Sexually Transmitted Infections & Antimicrobial PeptidesWilliam Shafer; Fiscal Year: 2009..abstract_text> ..
- Vascular tropism of Borrelia burgdorferiJenifer Coburn; Fiscal Year: 2005..The experiments that we are proposing will shed light on the significance of three B. burgdorferi proteins that may facilitate interactions of B. burgdorferi with the vasculature during the natural course of infection in mammals. ..
- ATP-DEPENDENT PROTEIN KINASES IN GRAM-POSITIVE BACTERIAMilton Saier; Fiscal Year: 2004..abstract_text> ..
- Effect of Neisserial Porin in Immune Cell ApoptosisLee Wetzler; Fiscal Year: 2006Neisserial porins (gonococcal Protein I [PIA or PIB] or meningococcal class 1 [PorA], 2 or 3 [PorB] proteins) are the major protein constituents of the Neisserial outer membrane...