amino acid transport systems

Summary

Summary: Cellular proteins and protein complexes that transport amino acids across biological membranes.

Top Publications

  1. ncbi A nutrient sensor mechanism controls Drosophila growth
    Julien Colombani
    Institute for Signaling, Developmental Biology and Cancer Research, UMR6543 CNRS Parc Valrose, 06108, Cedex 2, Nice, France
    Cell 114:739-49. 2003
  2. ncbi Nitrogen regulation in Saccharomyces cerevisiae
    Boris Magasanik
    Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Gene 290:1-18. 2002
  3. pmc A competitive inhibitor traps LeuT in an open-to-out conformation
    Satinder K Singh
    Vollum Institute, Oregon Health and Science University, 3181 Southwest Sam Jackson Park Road, Portland, OR 97239, USA
    Science 322:1655-61. 2008
  4. ncbi The Vam6 GEF controls TORC1 by activating the EGO complex
    Matteo Binda
    Department of Medicine, University of Fribourg, Switzerland
    Mol Cell 35:563-73. 2009
  5. ncbi The Gap1 general amino acid permease acts as an amino acid sensor for activation of protein kinase A targets in the yeast Saccharomyces cerevisiae
    Monica C V Donaton
    Laboratory of Molecular Cell Biology, Institute of Botany and Microbiology, Katholieke Universiteit Leuven, Belgium
    Mol Microbiol 50:911-29. 2003
  6. ncbi Arabidopsis AUX1 gene: a permease-like regulator of root gravitropism
    M J Bennett
    Department of Biological Sciences, University of Warwick, Coventry, CV4 7AL, UK
    Science 273:948-50. 1996
  7. ncbi Structure and mechanism of an amino acid antiporter
    Xiang Gao
    State Key Laboratory of Bio membrane and Membrane Biotechnology, Tsinghua University, Beijing 100084, China
    Science 324:1565-8. 2009
  8. pmc Amino acid transceptors: gate keepers of nutrient exchange and regulators of nutrient signaling
    Harinder S Hundal
    Division of Molecular Physiology, Sir James Black Centre, College of Life Sciences, University of Dundee, Dundee, DD1 5EH, UK
    Am J Physiol Endocrinol Metab 296:E603-13. 2009
  9. ncbi Transport and signaling via the amino acid binding site of the yeast Gap1 amino acid transceptor
    Griet Van Zeebroeck
    Laboratory of Molecular Cell Biology, Institute of Botany and Microbiology, Katholieke Universiteit Leuven, Kasteelpark Arenberg 31, B 3001 Leuven Heverlee, Flanders, Belgium
    Nat Chem Biol 5:45-52. 2009
  10. ncbi A conserved GTPase-containing complex is required for intracellular sorting of the general amino-acid permease in yeast
    Minggeng Gao
    Department of Biology, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA
    Nat Cell Biol 8:657-67. 2006

Detail Information

Publications301 found, 100 shown here

  1. ncbi A nutrient sensor mechanism controls Drosophila growth
    Julien Colombani
    Institute for Signaling, Developmental Biology and Cancer Research, UMR6543 CNRS Parc Valrose, 06108, Cedex 2, Nice, France
    Cell 114:739-49. 2003
    ..Our results demonstrate that the fat body functions as a nutrient sensor that restricts global growth through a humoral mechanism...
  2. ncbi Nitrogen regulation in Saccharomyces cerevisiae
    Boris Magasanik
    Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Gene 290:1-18. 2002
    ..A second focus of the review is the nitrogen regulation of the general amino acid permease, Gap1p, and the proline permease, Put4p, by ubiquitin mediated intracellular protein sorting in the secretory and endosomal pathways...
  3. pmc A competitive inhibitor traps LeuT in an open-to-out conformation
    Satinder K Singh
    Vollum Institute, Oregon Health and Science University, 3181 Southwest Sam Jackson Park Road, Portland, OR 97239, USA
    Science 322:1655-61. 2008
    ....
  4. ncbi The Vam6 GEF controls TORC1 by activating the EGO complex
    Matteo Binda
    Department of Medicine, University of Fribourg, Switzerland
    Mol Cell 35:563-73. 2009
    ..Thus, in addition to its regulatory role in homotypic vacuolar fusion and vacuole protein sorting within the HOPS complex, Vam6 also controls TORC1 function by activating the Gtr1 subunit of the EGO complex...
  5. ncbi The Gap1 general amino acid permease acts as an amino acid sensor for activation of protein kinase A targets in the yeast Saccharomyces cerevisiae
    Monica C V Donaton
    Laboratory of Molecular Cell Biology, Institute of Botany and Microbiology, Katholieke Universiteit Leuven, Belgium
    Mol Microbiol 50:911-29. 2003
    ....
  6. ncbi Arabidopsis AUX1 gene: a permease-like regulator of root gravitropism
    M J Bennett
    Department of Biological Sciences, University of Warwick, Coventry, CV4 7AL, UK
    Science 273:948-50. 1996
    ..The cloned AUX1 gene can restore the auxin-responsiveness of transgenic aux1 roots. Spatially, AUX1 is expressed in root apical tissues that regulate root gravitropic curvature...
  7. ncbi Structure and mechanism of an amino acid antiporter
    Xiang Gao
    State Key Laboratory of Bio membrane and Membrane Biotechnology, Tsinghua University, Beijing 100084, China
    Science 324:1565-8. 2009
    ..A conserved, acidic pocket opens to the periplasm. Structural and biochemical analysis reveals the essential ligand-binding residues, defines the transport route, and suggests a conserved mechanism for the antiporter activity...
  8. pmc Amino acid transceptors: gate keepers of nutrient exchange and regulators of nutrient signaling
    Harinder S Hundal
    Division of Molecular Physiology, Sir James Black Centre, College of Life Sciences, University of Dundee, Dundee, DD1 5EH, UK
    Am J Physiol Endocrinol Metab 296:E603-13. 2009
    ..We hypothesize (by extrapolation from knowledge of the yeast Ssy1 transceptor) that, at least for SNAT2, the transceptor discriminates between extracellular and intracellular amino acid stimuli when evoking a signal...
  9. ncbi Transport and signaling via the amino acid binding site of the yeast Gap1 amino acid transceptor
    Griet Van Zeebroeck
    Laboratory of Molecular Cell Biology, Institute of Botany and Microbiology, Katholieke Universiteit Leuven, Kasteelpark Arenberg 31, B 3001 Leuven Heverlee, Flanders, Belgium
    Nat Chem Biol 5:45-52. 2009
    ..They indicate that signaling requires a ligand-induced specific conformational change that may be part of but does not require the complete transport cycle...
  10. ncbi A conserved GTPase-containing complex is required for intracellular sorting of the general amino-acid permease in yeast
    Minggeng Gao
    Department of Biology, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA
    Nat Cell Biol 8:657-67. 2006
    ..Together, these studies provide evidence that the GSE complex has a key role in trafficking Gap1p out of the endosome and may serve as coat proteins in this process...
  11. pmc Cell surface polarization during yeast mating
    Michel Bagnat
    Max Planck Institute for Molecular Cell Biology and Genetics, 01307 Dresden, Germany
    Proc Natl Acad Sci U S A 99:14183-8. 2002
    ..Our results show that membrane microdomain clustering at the mating projection is involved in the generation and maintenance of polarity during mating...
  12. pmc Down-regulation of placental transport of amino acids precedes the development of intrauterine growth restriction in rats fed a low protein diet
    Nina Jansson
    Perinatal Center, Institute of Neuroscience and Physiology, Goteborg University, Sweden
    J Physiol 576:935-46. 2006
    ..Reduced maternal levels of insulin, leptin and IGF-1 may link maternal protein malnutrition to reduced fetal growth by down-regulation of key placental amino acid transporters...
  13. pmc Control of amino acid permease sorting in the late secretory pathway of Saccharomyces cerevisiae by SEC13, LST4, LST7 and LST8
    K J Roberg
    Department of Biology, Massachusetts Institute of Technology, Cambridge 02139, USA
    Genetics 147:1569-84. 1997
    ..The LST7 gene encodes a novel protein. Together, these data indicate that SEC13, LST4, LST7, and LST8 function in the regulated delivery of Gap1p to the cell surface, perhaps as components of a post-Golgi secretory-vesicle coat...
  14. ncbi Peptides induce persistent signaling from endosomes by a nutrient transceptor
    Marta Rubio-Texeira
    Laboratory of Molecular Cell Biology, Institute of Botany and Microbiology, KU Leuven, Flanders, Belgium
    Nat Chem Biol 8:400-8. 2012
    ..Hence, this work has identified specific dipeptides that cause enhanced proton influx through the Gap1 symporter, resulting in its defective vacuolar sorting, and independently transform it into a persistently signaling transceptor...
  15. pmc The TOR nutrient signalling pathway phosphorylates NPR1 and inhibits turnover of the tryptophan permease
    A Schmidt
    Department of Biochemistry, Biozentrum, University of Basel, CH 4056 Basel, Switzerland
    EMBO J 17:6924-31. 1998
    ..Thus, the TOR nutrient signalling pathway also controls growth by inhibiting a stationary phase (G0) programme. The control of NPR1 by TOR is analogous to the control of p70 s6 kinase and 4E-BP1 by mTOR in mammalian cells...
  16. ncbi Adaptation of plasma membrane amino acid transport mechanisms to physiological demands
    Stefan Broer
    School of Biochemistry and Molecular Biology, Faculty of Science, Australian National University, ACT 0200 Canberra, Australia
    Pflugers Arch 444:457-66. 2002
    ..Transport mechanisms are not conserved within families and may change with mutation of even a single amino acid residue in the transport protein. Thus transport mechanisms are easily adapted to physiological demands during evolution...
  17. ncbi Amino acid permeases in developing seeds of Vicia faba L.: expression precedes storage protein synthesis and is regulated by amino acid supply
    M Miranda
    Institut für Pflanzengenetik und Kulturpflanzenforschung IPK, Gatersleben, Germany
    Plant J 28:61-71. 2001
    ..Regulation of amino acid transport in relation to storage protein accumulation is discussed...
  18. pmc Amino acid sensing and mTOR regulation: inside or out?
    Deborah C I Goberdhan
    Department of Physiology, Anatomy and Genetics, University of Oxford, South Parks Road, Oxford OX1 3QX, UK
    Biochem Soc Trans 37:248-52. 2009
    ..The present review summarizes current ideas and suggests ways in which some of the models proposed might be unified to produce an amino acid detection system that can adapt to environmental change...
  19. ncbi GAP1, the general amino acid permease gene of Saccharomyces cerevisiae. Nucleotide sequence, protein similarity with the other bakers yeast amino acid permeases, and nitrogen catabolite repression
    J C Jauniaux
    Laboratoire de Microbiologie, Faculte des Sciences, Universite Libre de Bruxelles, Belgium
    Eur J Biochem 190:39-44. 1990
    ..J. Biochem. 133, 135-139]. Northern analysis of GAP1-specific transcripts in wild-type and in mutant strains is in agreement with these views and indicates that nitrogen catabolite repression of GAP1 synthesis occurs at the RNA level...
  20. ncbi Regulation of amino acid transporters by glucose and growth factors in cultured primary human trophoblast cells is mediated by mTOR signaling
    S Roos
    Perinatal Center, Dept of Physiology, Institute of Neuroscience and Physiology, Univ of Gothenburg, P O Box 432, SE 405 30 Gothenburg, Sweden
    Am J Physiol Cell Physiol 297:C723-31. 2009
    ..We conclude that the mTOR pathway represents an important intracellular regulatory link between nutrient and growth factor concentrations and amino acid transport in the human placenta...
  21. pmc Adaptation to diverse nitrogen-limited environments by deletion or extrachromosomal element formation of the GAP1 locus
    David Gresham
    Center for Genomics and Systems Biology and Department of Biology, New York University, New York, NY 10003, USA
    Proc Natl Acad Sci U S A 107:18551-6. 2010
    ..We propose that this genomic architecture facilitates evolvability of S. cerevisiae populations exposed to variation in levels and sources of environmental nitrogen...
  22. ncbi The Npr1 kinase controls biosynthetic and endocytic sorting of the yeast Gap1 permease
    J O De Craene
    Laboratoire de Physiologie Cellulaire, Institut de Biologie et de Médecine Moléculaires, Universite Libre de Bruxelles, 12 rue des Professeurs Jeneer et Brachet, 6041 Gosselies, Belgium
    J Biol Chem 276:43939-48. 2001
    ..Npr1 belongs to a subgroup of protein kinases, some of which are reported to exert a positive control on the activity of other permeases. We propose that these kinases also function as regulators of permease trafficking...
  23. ncbi Identification and functional characterization of a Na+-independent neutral amino acid transporter with broad substrate selectivity
    H Segawa
    Department of Pharmacology and Toxicology, Kyorin University School of Medicine, 6 20 2 Shinkawa, Mitaka, Tokyo 181 8611, Japan
    J Biol Chem 274:19745-51. 1999
    ....
  24. ncbi Increasing amino acid supply in pea embryos reveals specific interactions of N and C metabolism, and highlights the importance of mitochondrial metabolism
    Kathleen Weigelt
    Leibniz Institut für Pflanzengenetik und Kulturpflanzenforschung, Gatersleben, Germany
    Plant J 55:909-26. 2008
    ..These results demonstrate that legume seeds have a high capacity to regulate N:C ratios, and highlight the importance of mitochondria in the control of N-C balance and amino acid homeostasis...
  25. ncbi Amino acid transporters in the human placenta
    T Jansson
    Perinatal Center, Department of Physiology and Pharmacology, , , Sweden
    Pediatr Res 49:141-7. 2001
    ..The primary objective of this review is to integrate the available functional data on placental amino acid transport systems with recent molecular information on mammalian amino acid transporters...
  26. ncbi Conservation of amino acid transporters in fungi, plants and animals
    Daniel Wipf
    ZMBP, Plant Physiology, Auf der Morgenstelle 1, Eberhard Karls Universitat Tubingen, D 72076, Tubingen, Germany
    Trends Biochem Sci 27:139-47. 2002
    ..When used in combination with phylogenetic analyses, functional comparison might aid our prediction of physiological functions for related but uncharacterized open reading frames...
  27. pmc Adaptation of nutrient supply to fetal demand in the mouse involves interaction between the Igf2 gene and placental transporter systems
    Miguel Constância
    Laboratory of Developmental Genetics and Imprinting, The Babraham Institute, Babraham Research Campus, Cambridge CB2 4AT, United Kingdom
    Proc Natl Acad Sci U S A 102:19219-24. 2005
    ..Thus, crosstalk between an imprinted growth demand gene (Igf2) and placental supply transporter genes (Slc38a4, Slc38a2, and Slc2a3) may be a component of the genetic control of nutrient supply and demand during mammalian development...
  28. pmc Membrane chaperone Shr3 assists in folding amino acid permeases preventing precocious ERAD
    Jhansi Kota
    Ludwig Institute for Cancer Research, S 17177 Stockholm, Sweden
    J Cell Biol 176:617-28. 2007
    ..Consequently, in cells with impaired ERAD, AAPs are able to attain functional conformations independent of Shr3. These findings illustrate that folding and degradation are tightly coupled processes during membrane protein biogenesis...
  29. ncbi BAP2, a gene encoding a permease for branched-chain amino acids in Saccharomyces cerevisiae
    M Grauslund
    Department of Yeast Genetics, Carlsberg Laboratory, Copenhagen Valby, Denmark
    Biochim Biophys Acta 1269:275-80. 1995
    ....
  30. ncbi Mechanistic aspects of sodium-binding sites in LeuT-like fold symporters
    Camilo Perez
    Max Planck Institute of Biophysics, Structural Biology Department, Max von Laue Strasse 3, D 60438 Frankfurt Main, Germany
    Biol Chem 394:641-8. 2013
    ..Moreover, we highlight their crucial roles in conformational changes of LeuT-like fold transporters and their implication on a unifying mechanism in secondary transport...
  31. ncbi Substrate specificity and gene expression of the amino-acid permeases in Saccharomyces cerevisiae
    B Regenberg
    Department of Yeast Genetics, Carlsberg Laboratory, Copenhagen Valby, Denmark
    Curr Genet 36:317-28. 1999
    ..These findings modify the prevalent view that S. cerevisiae only contains one general amino-acid permease, Gap1p, and a number of permeases that are specific for a single or a few amino acids...
  32. pmc LAAT-1 is the lysosomal lysine/arginine transporter that maintains amino acid homeostasis
    Bin Liu
    Graduate Program in Chinese Academy of Medical Sciences and Peking Union Medical College, China
    Science 337:351-4. 2012
    ..Thus, LAAT-1 is the lysosomal lysine/arginine transporter, which suggests a molecular explanation for how cysteamine alleviates a lysosomal storage disease...
  33. pmc Concentrative sorting of secretory cargo proteins into COPII-coated vesicles
    Per Malkus
    Howard Hughes Medical Institute and Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA
    J Cell Biol 159:915-21. 2002
    ..Mutation of a diacidic sequence present in the COOH-terminal cytosolic domain of Gap1p eliminated concentrative sorting of this protein...
  34. ncbi Amino acid transporters in plants
    A Ortiz-Lopez
    Photosynthesis Research Unit, USDA Agricultural Research Service, University of Illinois Urbana Champaign, 190 ERML, Urbana, IL, USA
    Biochim Biophys Acta 1465:275-80. 2000
    ....
  35. pmc Amino acid transporters: roles in amino acid sensing and signalling in animal cells
    Russell Hyde
    Division of Molecular Physiology, MSI WTB complex, University of Dundee, Scotland, UK
    Biochem J 373:1-18. 2003
    ....
  36. pmc Identification of a disulfide bridge essential for transport function of the human proton-coupled amino acid transporter hPAT1
    Madlen Dorn
    Institute of Biochemistry Biotechnology, Faculty of Science I, Biozentrum, Martin Luther University Halle Wittenberg, Halle, Germany
    J Biol Chem 284:22123-32. 2009
    ..This disulfide bridge is very likely involved in forming or stabilizing the substrate binding site...
  37. ncbi Expression cloning and characterization of a transporter for large neutral amino acids activated by the heavy chain of 4F2 antigen (CD98)
    Y Kanai
    Department of Pharmacology and Toxicology, Kyorin University School of Medicine, 6 20 2 Shinkawa, Mitaka, Tokyo 181, Japan
    J Biol Chem 273:23629-32. 1998
    ..The cloning of LAT1 is expected to facilitate the research on the protein-protein interaction in the transporter field and to provide a clue to the search for still unidentified transporters...
  38. ncbi Alterations in the activity of placental amino acid transporters in pregnancies complicated by diabetes
    Thomas Jansson
    Department of Physiology and Pharmacology, The Perinatal Center, Goteborg University, Box 432, S 405 30 Goteborg, Sweden
    Diabetes 51:2214-9. 2002
    ..The increased MVM leucine uptake in the GDM/LGA group may contribute to accelerated fetal growth in these patients...
  39. ncbi The proton/amino acid cotransporter PAT2 is expressed in neurons with a different subcellular localization than its paralog PAT1
    Isabel Rubio-Aliaga
    Molecular Nutrition Unit, Technical University of Munich, Hochfeldweg 2, D 85350 Freising Weihenstephan, Germany
    J Biol Chem 279:2754-60. 2004
    ....
  40. ncbi Human L-type amino acid transporter 1 (LAT1): characterization of function and expression in tumor cell lines
    O Yanagida
    Second Department of Surgery, Kyorin University School of Medicine, Mitaka, Tokyo, Japan
    Biochim Biophys Acta 1514:291-302. 2001
    ..The properties of the hLAT1/h4F2hc complex would support the roles of this transporter in providing cells with essential amino acids for cell growth and cellular responses, and in distributing amino acid-related compounds...
  41. pmc Ssy1p and Ptr3p are plasma membrane components of a yeast system that senses extracellular amino acids
    H Klasson
    Ludwig Institute for Cancer Research, S 171 77 Stockholm, Sweden
    Mol Cell Biol 19:5405-16. 1999
    ..These findings suggest that Ssy1p and Ptr3p function in the PM as components of a sensor of extracellular amino acids...
  42. pmc Activity-dependent reversible inactivation of the general amino acid permease
    April L Risinger
    Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Mol Biol Cell 17:4411-9. 2006
    ....
  43. pmc LST8 negatively regulates amino acid biosynthesis as a component of the TOR pathway
    Esther J Chen
    Dept of Biology, Massachusetts Institute of Technology, 77 Massachusetts Ave, Cambridge, MA 02139, USA
    J Cell Biol 161:333-47. 2003
    ..Finally, the different effects of lst8 alleles on the activation of either the Rtg1/3p or Gln3p transcription factors reveal that these two pathways constitute distinct, genetically separable outputs of the Tor-Lst8 regulatory complex...
  44. pmc Molecular and functional characterization of a family of amino acid transporters from Arabidopsis
    Yan Hua Su
    Zentrum für Molekularbiologie der Pflanzen, Pflanzenphysiologie, Universität Tübingen, 72076 Tübingen, Germany
    Plant Physiol 136:3104-13. 2004
    ..CAT2 is localized to the tonoplast in transformed Arabidopsis protoplasts and thus may encode the long-sought vacuolar amino acid transporter...
  45. ncbi Novel motifs in amino acid permease genes from Leishmania
    Martin Akerman
    Department of Biology, Technion Israel Institute of Technology, Haifa 32000, Israel
    Biochem Biophys Res Commun 325:353-66. 2004
    ..Furthermore, the identification of genus-specific motifs in these proteins might be useful to better understand parasite physiology within its hosts...
  46. ncbi PAT-related amino acid transporters regulate growth via a novel mechanism that does not require bulk transport of amino acids
    Deborah C I Goberdhan
    Department of Human Anatomy and Genetics, University of Oxford, South Parks Road, Oxford OX1 3QX, UK
    Development 132:2365-75. 2005
    ..As PATH is likely to be saturated in vivo, we propose that one specialised function of high-affinity PAT-related molecules is to maintain growth as local nutrient levels fluctuate during development...
  47. pmc Arginine-agmatine antiporter in extreme acid resistance in Escherichia coli
    Ram Iyer
    Department of Biochemistry, Howard Hughes Medical Institute, Brandeis University, Waltham, Massachusetts 02454, USA
    J Bacteriol 185:6556-61. 2003
    ..Evidence for a lysine-dependent XAR system in E. coli is also presented. The protection by lysine, however, is milder than that by arginine...
  48. pmc Subcellular localization and adaptive up-regulation of the System A (SAT2) amino acid transporter in skeletal-muscle cells and adipocytes
    R Hyde
    Division of Molecular Physiology, School of Life Sciences, Medical Sciences Institute Wellcome Trust Biocentre Complex, University of Dundee, Dundee DD1 5EH, Scotland, UK
    Biochem J 355:563-8. 2001
    ....
  49. ncbi Amino acid transport across mammalian intestinal and renal epithelia
    Stefan Broer
    School of Biochemistry and Molecular Biology, Australian National University, Canberra, Australian Capital Territory, Australia
    Physiol Rev 88:249-86. 2008
    ..With very few exceptions, individual amino acids are transported by more than one transporter, providing backup capacity for absorption in the case of mutational inactivation of a transport system...
  50. pmc Curing of the [URE3] prion by Btn2p, a Batten disease-related protein
    Dmitry S Kryndushkin
    Laboratory of Biochemistry and Genetics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892 0830, USA
    EMBO J 27:2725-35. 2008
    ..Btn2p curing requires cell division, and our results suggest that Btn2p is part of a system, reminiscent of the mammalian aggresome, that collects aggregates preventing their efficient distribution to progeny cells...
  51. pmc ARAMEMNON, a novel database for Arabidopsis integral membrane proteins
    Rainer Schwacke
    Universitat zu Koln, Botanisches Institut, Gyrhofstrasse 15, 50931 Koln, Germany
    Plant Physiol 131:16-26. 2003
    ..and incorporated in the ARAMEMNON DB. The ARAMEMNON DB is accessible at the URL http://aramemnon.botanik.uni-koeln.de...
  52. ncbi Function and structure of heterodimeric amino acid transporters
    C A Wagner
    Department of Cellular and Molecular Physiology, School of Medicine, Yale University, New Haven, Connecticut 06520, USA
    Am J Physiol Cell Physiol 281:C1077-93. 2001
    ....
  53. ncbi Carbon catabolite repression regulates amino acid permeases in Saccharomyces cerevisiae via the TOR signaling pathway
    George J Peter
    Institute of Urology and Nephrology, University College London, 67 Riding House Street, London W1W 7EY, United Kingdom
    J Biol Chem 281:5546-52. 2006
    ..Our results provide novel insights into the regulation of yeast amino acid permeases and signaling mechanisms involved in this regulation...
  54. pmc Two FK506 resistance-conferring genes in Saccharomyces cerevisiae, TAT1 and TAT2, encode amino acid permeases mediating tyrosine and tryptophan uptake
    A Schmidt
    Department of Biochemistry, Biozentrum, University of Basel, Switzerland
    Mol Cell Biol 14:6597-606. 1994
    ..TAT2 is the tryptophan high-affinity transporter. FK506 does not reduce the levels of TAT1 and TAT2 transcripts, indicating that the inhibition of amino acid transport by the drug is posttranscriptional...
  55. ncbi The amino acid permease AAP8 is important for early seed development in Arabidopsis thaliana
    Roberto Schmidt
    Plant Physiology, Zentrum für Molekularbiologie der Pflanzen, Auf der Morgenstelle 1, 72076 Tubingen, Germany
    Planta 226:805-13. 2007
    ..AAP8 plays a crucial role for the uptake of amino acids into the endosperm and supplying the developing embryo with amino acids during early embryogenesis...
  56. ncbi Polyamine uptake by DUR3 and SAM3 in Saccharomyces cerevisiae
    Takeshi Uemura
    Graduate School of Pharmaceutical Sciences, Chiba University, 1 8 1 Inohana, Chuo Ku, Chiba 260 8675, Japan
    J Biol Chem 282:7733-41. 2007
    ..It was found that DUR3 (but not SAM3) was activated by phosphorylation of Thr(250), Ser(251), and Thr(684) by polyamine transport protein kinase 2...
  57. pmc Systematic mutational analysis of the intracellular regions of yeast Gap1 permease
    Ahmad Merhi
    Physiologie Moléculaire de la Cellule, Universite Libre de Bruxelles, Gosselies, Belgium
    PLoS ONE 6:e18457. 2011
    ..The NT of Gap1 contains the acceptor lysines for ubiquitylation and its CT includes a sequence essential to exit from the endoplasmic reticulum (ER)...
  58. ncbi Regulation of placental amino acid transporter activity by mammalian target of rapamycin
    S Roos
    Perinatal Center, Department of Physiology, Institute of Neuroscience and Physiology, University of Gothenburg, PO Box 432, Gothenburg SE 405 30, Sweden
    Am J Physiol Cell Physiol 296:C142-50. 2009
    ..These data suggest that mTOR regulates placental amino acid transporters by posttranslational modifications or by affecting transporter translocation to the plasma membrane...
  59. pmc Amino acid transporters are localized to transfer cells of developing pea seeds
    M Tegeder
    Plant Physiology, Zentrum für Molekularbiologie der Pflanzen, Universitat Tubingen, D 72076 Tubingen, Germany
    Plant Physiol 122:319-26. 2000
    ..Overall, our observations suggest that this transporter, which is localized in transfer cells of cotyledons, might play a role in the uptake of the full spectrum of amino acids released from seed coats...
  60. pmc A mutation in amino acid permease AAP6 reduces the amino acid content of the Arabidopsis sieve elements but leaves aphid herbivores unaffected
    Emma Hunt
    CABI Europe Switzerland, Delémont, CH 2800, Switzerland
    J Exp Bot 61:55-64. 2010
    ..The data are discussed in relation to the regulation of phloem composition and the role of phloem amino acids in regulating aphid performance...
  61. ncbi Regulatory mechanisms of SNAT2, an amino acid transporter, in L6 rat skeletal muscle cells by insulin, osmotic shock and amino acid deprivation
    Hitoshi Kashiwagi
    Department of Pharmacy, Hokkaido University Hospital, Sapporo, Hokkaido, Japan
    Amino Acids 36:219-30. 2009
    ..Western blot and RT-PCR analysis showed an increase in system A at the protein and mRNA levels with each stimulation...
  62. pmc Proton-assisted amino-acid transporters are conserved regulators of proliferation and amino-acid-dependent mTORC1 activation
    S Heublein
    Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford, UK
    Oncogene 29:4068-79. 2010
    ..Therefore our data are consistent with a model in which PATs modulate the activity of mTORC1 not by transporting amino acids into the cell but by modulating the intracellular response to amino acids...
  63. ncbi Ubiquitin is required for sorting to the vacuole of the yeast general amino acid permease, Gap1
    O Soetens
    Laboratoire de Physiologie Cellulaire, , , 12 rue des Professeurs Jeneer et Brachet, 6041 Gosselies, Belgium
    J Biol Chem 276:43949-57. 2001
    ..This result reinforces the growing evidence that ubiquitin plays an important role not only in internalization of plasma membrane proteins but also in their sorting in the endosomes and/or trans-Golgi...
  64. pmc Ancestry and progeny of nutrient amino acid transporters
    Dmitri Y Boudko
    The Whitney Laboratory for Marine Bioscience, University of Florida, 9505 Ocean Shore Boulevard, St Augustine, FL 32080, USA
    Proc Natl Acad Sci U S A 102:1360-5. 2005
    ....
  65. pmc Origins of amino acid transporter loci in trypanosomatid parasites
    Andrew P Jackson
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridgeshire, CB10 1SA, UK
    BMC Evol Biol 7:26. 2007
    ..This study provides a comprehensive phylogenetic account of the origins of these genes, redefining each locus according to a positional criterion, through the integration of phyletic identity with comparative gene order information...
  66. ncbi Aromatic amino acid transporter AAT-9 of Caenorhabditis elegans localizes to neurons and muscle cells
    Emilija Veljkovic
    Institutes of Physiology and Zoology, University of Zurich, Winterthurerstrasse 190, CH 8057 Zurich, Switzerland
    J Biol Chem 279:49268-73. 2004
    ..Taken together, the results show that AAT-9 is expressed in excitable cells of the nematode head and pharynx in which it may provide a pathway for aromatic amino acid transport...
  67. pmc TOR coordinates bulk and targeted endocytosis in the Drosophila melanogaster fat body to regulate cell growth
    Krista M Hennig
    Department of Genetics, Cell Biology, and Development, University of Minnesota, Minneapolis, MN 55455, USA
    J Cell Biol 173:963-74. 2006
    ..Our data indicate that endocytosis acts both as an effector function downstream of TOR and as a physiologically relevant regulator of TOR signaling...
  68. pmc Transport activity-dependent intracellular sorting of the yeast general amino acid permease
    Natalie E Cain
    Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Mol Biol Cell 22:1919-29. 2011
    ..These results indicate a parsimonious regulatory mechanism by which Gap1p senses its transport substrates to set an appropriate level of transporter activity at the cell surface...
  69. ncbi Anticancer agent E7070 inhibits amino acid and uracil transport in fission yeast
    K Tsukahara
    Tsukuba Research Laboratories, Eisai Co, Ltd, Ibaraki, Japan
    Mol Pharmacol 60:1254-9. 2001
    ..Thus, some of the molecular targets for E7070 action in S. pombe are likely to be leucine and uracil transporters...
  70. ncbi Renal amino acid transport systems and essential hypertension
    Vanda Pinto
    Department of Pharmacology and Therapeutics, Faculty of Medicine, University of Porto, 4200 319 Porto, Portugal
    FASEB J 27:2927-38. 2013
    ..The study of the regulation of renal amino acid transporters may help to define the underlying mechanisms predisposing individuals to an increased risk for development of hypertension...
  71. ncbi Role of the sRNA GcvB in regulation of cycA in Escherichia coli
    Sarah C Pulvermacher
    Department of Microbiology, University of Iowa, Iowa City, IA 52242, USA
    Microbiology 155:106-14. 2009
    ..However, mutations predicted to disrupt base-pairing between cycA mRNA and GcvB did not alter expression of cycA : : lacZ. A model for GcvB function in cell physiology is discussed...
  72. ncbi Transport of amino acids through the placenta and their role
    M A Grillo
    Dipartimento di Medicina e Oncologia Sperimentale, Sezione di Biochimica, Universita di Torino, Via Michelangelo 27, 10126 Torino, Italy
    Amino Acids 34:517-23. 2008
    ..This occurs for instance for arginine, which gives rise to polyamines and to NO. Interconversion occurs among few other amino acids Transport is altered in pregnancy complications, such as restricted fetal growth...
  73. pmc The SLC36 family of proton-coupled amino acid transporters and their potential role in drug transport
    David T Thwaites
    Epithelial Research Group, Institute for Cell and Molecular Biosciences, Faculty of Medical Sciences, Newcastle University, Newcastle upon Tyne, UK
    Br J Pharmacol 164:1802-16. 2011
    ....
  74. pmc Evidence for coupled biogenesis of yeast Gap1 permease and sphingolipids: essential role in transport activity and normal control by ubiquitination
    Elsa Lauwers
    Laboratoire de Physiologie Moléculaire de la Cellule, Institut de Biologie et de Médecine Moléculaires, Universite Libre de Bruxelles, B 6041 Gosselies, Belgium
    Mol Biol Cell 18:3068-80. 2007
    ..We propose that coupled biogenesis of Gap1 and SLs would create an SL microenvironment essential to the normal conformation, function, and control of ubiquitination of the permease...
  75. ncbi Characterization of anionic amino acid transport systems in mouse mammary gland
    V K Kansal
    Division of Animal Biochemistry, National Dairy Research Institute, Karnal 132 001, India
    Indian J Exp Biol 38:1097-103. 2000
    ..Culturing pregnant mouse mammary tissue explants in vitro in the presence of lactogenic hormones (insulin plus cortisol plus prolactin) did not affect appreciably the uptake of L-glutamate...
  76. ncbi SHR3: a novel component of the secretory pathway specifically required for localization of amino acid permeases in yeast
    P O Ljungdahl
    Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142
    Cell 71:463-78. 1992
    ..shr3 mutants constitutively express elevated levels of GCN4, and mutant shr3/shr3 diploids undergo dimorphic transitions that result in filamentous growth at enhanced frequencies...
  77. ncbi Substrate specificity and expression profile of amino acid transporters (AAPs) in Arabidopsis
    W N Fischer
    Institut fur Genbiologische Forschung, Berlin, Germany
    J Biol Chem 270:16315-20. 1995
    ..The specific distribution in the plant and the different substrate specificities of AAP transporters may indicate that tissues differ both qualitatively and quantitatively regarding import or export of amino acids...
  78. pmc Siliques are Red1 from Arabidopsis acts as a bidirectional amino acid transporter that is crucial for the amino acid homeostasis of siliques
    Friederike Ladwig
    Zentrum für Molekularbiologie der Pflanzen, Plant Physiology, D 72076 Tuebingen, Germany
    Plant Physiol 158:1643-55. 2012
    ..Our data demonstrate that the SIAR1-mediated export of amino acids plays an important role in organic nitrogen allocation and particularly in amino acid homeostasis in developing siliques...
  79. ncbi The amino acid permeases AAP3 and AAP6 are involved in root-knot nematode parasitism of Arabidopsis
    Heather H Marella
    Donald Danforth Plant Science Center, St Louis, MO, USA
    Mol Plant Microbe Interact 26:44-54. 2013
    ..The transport of amino acids by AAP3 and AAP6 is important for nematode infection and success of the progeny...
  80. ncbi A cryptic role of a glycolytic-gluconeogenic enzyme (aldolase) in amino acid transporter turnover in Aspergillus nidulans
    Katerina Roumelioti
    Institute of Biology, National Center for Scientific Research, Demokritos, Athens, Greece
    Fungal Genet Biol 47:254-67. 2010
    ..Our results support a novel role of the FbaA protein that is, involvement in the regulation of amino acids transporters...
  81. ncbi Salt-dependent expression of a nitrate transporter and two amino acid transporter genes in Mesembryanthemum crystallinum
    Olga V Popova
    Department of Physiology and Biochemistry of Plants, Faculty of Biology, University of Bielefeld, 33615 Bielefeld, Germany
    Plant Mol Biol 52:569-78. 2003
    ....
  82. pmc Salt stress-induced proline transporters and salt stress-repressed broad specificity amino acid permeases identified by suppression of a yeast amino acid permease-targeting mutant
    D Rentsch
    Institut fur Genbiologische Forschung, Berlin, Germany
    Plant Cell 8:1437-46. 1996
    ..These results corroborate the general finding that under water stress, amino acid export is impaired whereas proline export is increased...
  83. pmc Ectopic expression of an amino acid transporter (VfAAP1) in seeds of Vicia narbonensis and pea increases storage proteins
    Hardy Rolletschek
    Institut fur Pflanzengenetik und Kulturpflanzenforschung, D 06466 Gatersleben, Germany
    Plant Physiol 137:1236-49. 2005
    ..We conclude that seed protein synthesis is nitrogen limited and that seed uptake activity for nitrogen is rate limiting for storage protein synthesis...
  84. ncbi Intracellular trafficking and ubiquitination of the Schizosaccharomyces pombe amino acid permease Aat1p
    Mai Nakase
    Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, Hakozaki, Fukuoka, Japan
    Microbiology 158:659-73. 2012
    ..These results indicate that ubiquitination is an important determinant for localization and regulation of the Aat1p permease in S. pombe...
  85. pmc Structure of a prokaryotic virtual proton pump at 3.2 A resolution
    Yiling Fang
    Department of Biochemistry, Howard Hughes Medical Institute, Brandeis University, Waltham, Massachusetts 02454, USA
    Nature 460:1040-3. 2009
    ..2 A resolution. The protein is captured in an outward-open, substrate-free conformation with transmembrane architecture remarkably similar to that seen in four other families of apparently unrelated transport proteins...
  86. ncbi The product of the SHR3 orthologue of Aspergillus nidulans has restricted range of amino acid transporter targets
    Z Erpapazoglou
    Institute of Biology, National Center for Scientific Research, Demokritos NCSR D, Aghia Paraskevi, 153 10 Athens, Greece
    Fungal Genet Biol 43:222-33. 2006
    ..Strongly reduced membrane levels of a PrnB-sGFP fusion in a shrAdelta background clearly suggest a direct role of ShrA in the topogenesis of the proline specific transporter...
  87. ncbi PtAAP11, a high affinity amino acid transporter specifically expressed in differentiating xylem cells of poplar
    Jeremy Couturier
    UMR INRA UHP 1136 Interactions Arbres Microorganismes, IFR 110 Ecosystèmes Forestiers, Agroressources, Bioprocédés et Alimentation, Nancy University, Faculte des Sciences et Techniques, BP 70239, F 54506 Vandoeuvre les Nancy Cedex, France
    J Exp Bot 61:1671-82. 2010
    ..The present study provides important information highlighting the role of a specific amino acid transporter in xylogenesis in poplar...
  88. pmc Brief hyperglycaemia in the early pregnant rat increases fetal weight at term by stimulating placental growth and affecting placental nutrient transport
    Anette Ericsson
    Perinatal Center, Institute of Neuroscience and Physiology, Gothenburg University, Box 432, S 405 30 Gothenburg, Sweden
    J Physiol 581:1323-32. 2007
    ..We speculate that maternal metabolism in early pregnancy represents a key environmental cue to which the placenta responds in order to match fetal growth rate with the available resources of the mother...
  89. ncbi Modelling and mutational evidence identify the substrate binding site and functional elements in APC amino acid transporters
    Ioannis Vangelatos
    Institute of Biology, National Center for Scientific Research Demokritos, Aghia Paraskevi 153 10, Athens, Greece
    Mol Membr Biol 26:356-70. 2009
    ....
  90. ncbi Isoforms of amino acid transporters in placental syncytiotrophoblast: plasma membrane localization and potential role in maternal/fetal transport
    R Cariappa
    Department of Pediatrics, Washington University School of Medicine and St Louis Children s Hospital, Box 8116 One Children s Place, St Louis, MO 63110, USA
    Placenta 24:713-26. 2003
    ..We hope that this review will stimulate investigation of the properties of trophoblast transporter isoforms, their membrane localization and their contribution to the cellular mechanism of maternal-fetal nutrient transport...
  91. pmc SLC36A4 (hPAT4) is a high affinity amino acid transporter when expressed in Xenopus laevis oocytes
    Samyuktha Muralidharan Pillai
    School of Life Sciences, Oxford Brookes University, Oxford OX3 0BP, United Kingdom
    J Biol Chem 286:2455-60. 2011
    ....
  92. pmc Identification and characterization of a lysosomal transporter for small neutral amino acids
    C Sagne
    Institut National de la Santé et de la Recherche Médicale U 513, CHU Henri Mondor, 8 rue du General Sarrail, 94010 Creteil Cedex, France
    Proc Natl Acad Sci U S A 98:7206-11. 2001
    ..Homology searching in eukaryotic genomes suggests that LYAAT-1 defines a subgroup of lysosomal transporters in the amino acid/auxin permease family...
  93. pmc Amino acid transport through the Saccharomyces cerevisiae Gap1 permease is controlled by the Ras/cAMP pathway
    Jinnie M Garrett
    Department of Biology, Hamilton College, 198 College Hill Road, Clinton, NY 13323, USA
    Int J Biochem Cell Biol 40:496-502. 2008
    ..A mechanism by which the Ras2/cAMP/PKA pathway controls the ubiquitin-dependent degradation of Gap1p is most consistent with the data...
  94. pmc Amino acid transporters and nutrient-sensing mechanisms: new targets for treating insulin-linked disorders?
    B Reynolds
    Department of Physiology, Anatomy and Genetics, Le Gros Clark Building, University of Oxford, South Parks Road, Oxford OX1 3QX, U K
    Biochem Soc Trans 35:1215-7. 2007
    ..As our understanding of how nutrient sensing has an impact on IIS/TOR increases, novel targets to modulate aberrant IIS in disease are likely to emerge, which could complement current strategies designed to block kinases in this pathway...
  95. ncbi Leucine induces phosphorylation and activation of p70S6K in cortical neurons via the system L amino acid transporter
    Yuta Ishizuka
    Department of Molecular Neurobiology, Brain Research Institute, Niigata University, Niigata, Japan
    J Neurochem 106:934-42. 2008
    ..We propose that leucine plays important roles in regulating signaling by p70S6 kinase by acting as an intercellular communicator in the CNS...
  96. pmc Seed and vascular expression of a high-affinity transporter for cationic amino acids in Arabidopsis
    W B Frommer
    Institut fur Genbiologische Forschung, Berlin, Germany
    Proc Natl Acad Sci U S A 92:12036-40. 1995
    ..AAT1 is specifically expressed in major veins of leaves and roots and in various floral tissues--i.e., and developing seeds...
  97. pmc Maternal protein restriction in the rat inhibits placental insulin, mTOR, and STAT3 signaling and down-regulates placental amino acid transporters
    Fredrick J Rosario
    Center for Pregnancy and Newborn Research, Department of Obstetrics and Gynecology, University of Texas Health Science Center San Antonio, Mail Code 7836, 7703 Floyd Curl Drive, San Antonio, Texas 78229 3900, USA
    Endocrinology 152:1119-29. 2011
    ..We speculate that maternal endocrine and metabolic control of placental nutrient transport reduces fetal growth in response to protein restriction...
  98. pmc K63-linked ubiquitin chains as a specific signal for protein sorting into the multivesicular body pathway
    Elsa Lauwers
    Laboratoire de Physiologie Moléculaire de la Cellule, Institut de Biologie et de Médecine Moléculaires, Universite Libre de Bruxelles, Gosselies, Belgium
    J Cell Biol 185:493-502. 2009
    ..Our data reveal that K63-linked Ub chains act as a specific signal for MVB sorting, providing further insight into the Ub code of membrane protein trafficking...
  99. ncbi COPII-cargo interactions direct protein sorting into ER-derived transport vesicles
    M J Kuehn
    Department of Molecular and Cell Biology, Howard Hughes Medical Institute, University of California, Berkeley 94720, USA
    Nature 391:187-90. 1998
    ..Our results indicate that cargo packaging signals and soluble cargo adaptors are recognized by a recruitment complex comprising Sar1-GTP and Sec23/24...
  100. ncbi Transporters for uptake and allocation of organic nitrogen compounds in plants
    Doris Rentsch
    University of Bern, Institute of Plant Sciences, Altenbergrain 21, 3011 Bern, Switzerland
    FEBS Lett 581:2281-9. 2007
    ..The review summarizes our current understanding of the molecular mechanisms of organic nitrogen transport processes, with a focus on amino acid, ureide and peptide transporters...
  101. pmc Inhibition of cystine uptake disrupts the growth of primary brain tumors
    Wook Joon Chung
    Department of Neurobiology, Civitan International Research Center, The University of Alabama, Birmingham, Alabama 35294 0021, USA
    J Neurosci 25:7101-10. 2005
    ..These data suggest that inhibition of cystine uptake into glioma cells through the pharmacological inhibition of system xc- may be a viable therapeutic strategy with a Food and Drug Administration-approved drug already in hand...

Research Grants63

  1. Arginine Availability and Metabolism in the Immune Evasion of H. pylori
    Keith T Wilson; Fiscal Year: 2013
    ..These studies will have a major impact on our understanding of H. pylori immunopathogenesis and gastric carcinogenesis, and will lead to new approaches for risk assessment and treatment. ..
  2. EFFECT OF DRUGS OF ABUSE ON NEURONAL REDOX AND METHYLATION STATUS
    Richard C Deth; Fiscal Year: 2012
    ..The results will provide an entirely new way of thinking about how drugs of abuse affect the brain and could lead to improved ways to treatment to treat drug addiction. ..
  3. Development of a mouse model of obesity in pregnancy
    Thomas Jansson; Fiscal Year: 2009
    ....
  4. Catalytic inactivation of miRNA function by customized RNase P-based ribozymes
    DANIEL R contact SCHOENBERG; Fiscal Year: 2010
    ..This proposal describes a new approach to inactivating miR-122 using a catalytic RNA enzyme. The long term goal is to apply this strategy to the treatment of HCV infection. ..
  5. Immunomodulatory effects of arginine supplementation in colitis and colon cancer
    Keith T Wilson; Fiscal Year: 2013
    ..Through investigation of epithelial and immune function, these studies seek to provide new macronutrient- based strategies for treatment of IBD and prevention of colitis-associated dysplasia and carcinoma. ..
  6. NITRIC OXIDE AND OXYGENATION IN DIABETIC KIDNEYS
    NILS AXEL FREDRIK PALM; Fiscal Year: 2010
    ..abstract_text> ..
  7. Identification of novel system xc- inhibitors
    BARBARA STAUCH SLUSHER; Fiscal Year: 2012
    ....
  8. smFRET Investigation of Gating in a Neurotransmitter Transporter Homolog
    RACHEL ANN KOLSTER; Fiscal Year: 2013
    ..Furthermore, the single-molecule methods developed in this proposal will aid in the establishment of a platform for single-molecule imaging of other membrane proteins, including the human NSS, in situ on the cell membrane. ..
  9. Alcohol Abuse and HIV-1: Mechanisms of Combined CNS Injury and Interventions
    Yuri Persidsky; Fiscal Year: 2012
    ..Current proposal aims to understand mechanisms of combined effects of HIV-1 and alcohol abuse in the brain and to propose neuroprotective therapies. ..
  10. Maternal pro-inflammatory status in obesity regulates placental function
    Theresa L Powell; Fiscal Year: 2013
    ..This is highly relevant to public health since this new information may lead to novel intervention strategies to alleviate fetal overgrowth, which could lessen the epidemic of obesity and diabetes in the next generation. ..
  11. Extracellular pH modulation by Candida albicans in vitro and in vivo
    Michael C Lorenz; Fiscal Year: 2013
    ..Finally, we will use live cell imaging to dissect the intracellular trafficking of C. albicans in the macrophage and determine the role of alkalinization and ammonia release in modulating endocytic maturation. ..
  12. Mechanisms and Interventions for Methamphetamine and HIV-1 Induced CNS Injury
    Yuri Persidsky; Fiscal Year: 2013
    ..abstract_text> ..
  13. Initiation and Regulation of Chronic Autoimmune Prostate Inflammation
    Timothy L Ratliff; Fiscal Year: 2013
    ....
  14. Role of System xc in Asbestos Induced Autoimmune Responses
    JEAN COOPER PFAU; Fiscal Year: 2010
    ..Because asbestos causes oxidative stress, this project will test the hypothesis that System xc- plays a major role in the altered immune responses that lead to autoantibody production. ..
  15. Altered transport and epigenomic changes in maneb-potentiated neurotoxicity
    JAMES R ROEDE; Fiscal Year: 2012
    ..This project is designed to test this hypothesis. ..
  16. Altered transport and epigenomic changes in maneb-potentiated neurotoxicity
    JAMES R ROEDE; Fiscal Year: 2013
    ..The proposed research will meet these goals and foster the development of my independent research career. ..
  17. Olivocerebellar Circuitry in Autism
    Gene J Blatt; Fiscal Year: 2010
    ..abstract_text> ..
  18. mechanisms of HIV and drug abuse pathogenesis in CNS
    Lena Al-Harthi; Fiscal Year: 2013
    ....
  19. Molecular Pathogenesis of Skeletal Muscle Atrophy
    Christopher M Adams; Fiscal Year: 2013
    ..abstract_text> ..
  20. Ethanol Dependence, Drinking &Mechanisms Regulating Accumbal Glutamate Activity
    Vorani Ramachandra; Fiscal Year: 2013
    ..abstract_text> ..
  21. ROLE OF CD98 IN INTESTINAL PERMEABILITY
    Didier Merlin; Fiscal Year: 2012
    ..S, suffer from inflammatory bowel disease. New therapeutic strategies based on a better understanding of the pathogenesis of IBD will improve the clinical care of patient with this disorder. ..
  22. Molecular Mechanisms Regulating Placental Nutrient Transporters
    Thomas Jansson; Fiscal Year: 2013
    ..Furthermore, we propose a novel model for the regulation of amino acid transporters in the human placenta. ..
  23. Control of nuclear thyroid hormone signaling by cell surface proteins
    DANIEL RICHARD BUCHHOLZ; Fiscal Year: 2010
    ..Thus, knowledge of developmental roles of LAT1 is important when considering use of drugs targeting LAT1 to evaluate potential benefits and side-effects. ..
  24. Translational Control by Nutrients
    Maria Hatzoglou; Fiscal Year: 2010
    ..The proposed studies aim to identify these proteins and characterize how they activate the IRES. This is the first report on the regulation of the activity of the IRES by nutrients. ..
  25. Novel Chemotherapeutics for Brain Tumors
    Bernd Jandeleit; Fiscal Year: 2013
    ..In projected Phase 2 studies, successful compounds will be tested in vivo in validated xenograft models for various brain tumors. ..
  26. Molecular Genetics of Regulated Protein Delivery of the Plasma Membrane
    CHRIS ALAN KAISER; Fiscal Year: 2010
    ..A final aim of the project is (iv) to understand how ubiquitination is regulated and to identify the cellular components required for recognition of the ubiquitin tag. ..
  27. Regulation of System xc- by Interleukin-1 beta
    NICOLE ALYSE JACKMAN; Fiscal Year: 2010
    ..Successful completion of this proposal will advance and refine our knowledge about an important new therapeutic target (system xc-) that complements other ongoing efforts to reduce injury associated with oxidative stress. ..
  28. Nutrient regulation of amino acid transporters in aging human skeletal muscle
    Micah J Drummond; Fiscal Year: 2013
    ..The pilot data collected will be used for future R01 submissions to identify mechanisms and novel targets for interventions to improve muscle function and preserve physical independence in older adults. ..
  29. Development of Novel Fluorinated Amino Acids
    Mark Goodman; Fiscal Year: 2004
    ..carrier-mediated facilitated transport involving either the "L" large-neutral amino acid or "A" alanine amino acid transport systems. Preliminary evaluation of amino acids labeled with positron emitters and which are substrates for the "..
  30. Emory Molecular and Translational Imaging Center
    Carolyn C Meltzer; Fiscal Year: 2012
    ..abstract_text> ..
  31. Regulation of System xc- by Interleukin-1Beta
    Nicole Jackman; Fiscal Year: 2009
    ..Successful completion of this proposal will advance and refine our knowledge about an important new therapeutic target (system xc-) that complements other ongoing efforts to reduce neurological injury associated with oxidative stress. ..
  32. Characterization of amino acid transport- and signaling in the mosquito fat body
    Immo Alex Hansen; Fiscal Year: 2013
    ..In addition, this study will expand our knowledge of the functional mechanism of cationic amino acid transporter in insects with implications for understanding essential amino acid transporters in humans and other model systems. ..
  33. A Controlled Trial of NAC for Cocaine Dependence
    Robert Malcolm; Fiscal Year: 2009
    ..placebo;and 3) to study cocaine craving/cue reactivity and withdrawal symptoms in subjects taking NAC or placebo. ..
  34. Regulation of GABAergic Neuron Differentiation
    Brian Condie; Fiscal Year: 2005
    ..The proposed experiments will lead to new insights into the co-ordinate regulation of GABAergic neuron differentiation during fetal development. ..
  35. GLUTAMATE TRANSPORT IN EXCITOTOXIC CELL DEATH
    Margaret Sutherland; Fiscal Year: 1999
    ..Primary cortical cultures from wild type (+/+) and EAAT2 transgenic mice will be used to quantitate changes in neuronal survival under normal and hypoxic conditions (Specific Aim 2). ..
  36. NEW RETROVIRAL RECEPTORS/HOST RESISTANCES
    David Kabat; Fiscal Year: 2004
    ..These investigations build on a technological breakthrough and will substantially expand our understanding of human and animal retroviral diseases and of host resistance mechanisms. ..
  37. Control of Cardiac Metabolism: NADH Shuttles/Hypertrophy
    Thomas Scholz; Fiscal Year: 2006
    ..A complete understanding of this pathway is essential to fully define the interaction between these two compartments. [unreadable] [unreadable]..
  38. Microvascular Regulation During Intestinal Absorption
    HAROLD G BOHLEN; Fiscal Year: 2010
    ..A substantial fraction of the NO in blood may be from NO loading in the small arteries based on their very high wall NO concentration and represents a new form of vascular regulation. ..
  39. DEVELOPMENT OF AMINO ACID TRANSPORT IN EARLY EMBRYOS
    LON VAN WINKLE; Fiscal Year: 1993
    The purpose of the proposed studies is to examine the interrelationships among amino acid transport systems in mouse oocytes, ova and preimplantation conceptuses and intra-and extracellular amino acid concentrations...
  40. AMINO ACID TRANSPORTER B: PHARMACOLOGY & THERAPEUTIC USE
    Malliga Ganapathy; Fiscal Year: 2006
    ....
  41. BLOOD BRAIN BARRIER LARGE NEUTRAL AMINO ACID TRANSPORTER
    William Pardridge; Fiscal Year: 2003
    ..These studies will provide new molecular biological information on a crucial transporter at the blood-brain barrier that regulates the supply in the brain of essential amino acids. ..
  42. HYPOXIA INHIBITS L-ARGININE UPTAKE BY LUNG ENDOTHELIUM
    Edward Block; Fiscal Year: 2007
    ..unreadable]..
  43. DEVELOPMENT OF NEUROPROTECTIVE DRUGS FOR RETINAL DISEASE
    Scott Eliasof; Fiscal Year: 2000
    ..abstract_text> ..
  44. Function and High-Resolution of an APC superfamily amino acid transporter
    Bliss Forbush; Fiscal Year: 2009
    ....
  45. MECHANISMS OF INHERITED MEMBRANE TRANSPORT DISORDERS
    Stanton Segal; Fiscal Year: 1992
    ..Bovee and Segal, Project 5, Examination of Human Intestinal Amino Acid Transport Systems, Dr. McNamara, Project 6, Ontogeny and Regulation of Transport in Renal Membranes, Dr...
  46. YEAST AMINO ACID PERMEASES
    JANETTA GARRETT; Fiscal Year: 2000
    ..Tryptophan uptake is of particular interest because there are many unusual phenotypes associated with low tryptophan levels in yeast. There are also interesting parallels between yeast tryptophan uptake and auxin response in plants. ..
  47. NITRIC OXIDE AND OXYGENATION IN DIABETIC KIDNEYS
    NILS PALM; Fiscal Year: 2007
    ..unreadable] [unreadable]..
  48. FUNCTIONS OF RETROVIRAL RECEPTORS TRANSPORTERS
    David Kabat; Fiscal Year: 2001
    ..5) Analyze the role of glycosylation in blocking infection of hamster cells by ecotropic and amphotropic mouse retroviruses. ..
  49. STRUCTURE AND FUNCTION OF MEMBRANE PROTEINS
    William Dowhan; Fiscal Year: 2004
    ..The proposed experiments are expected to define at the molecular level the role of PE and other lipids in the assembly, organization, and function of polytopic membrane proteins. ..
  50. Role of arginase in allergic airway inflammation
    MARC ROTHENBERG; Fiscal Year: 2007
    ..It is anticipated that the proposed studies will shed new light on the pathogenesis of experimental asthma and provide a rationale for possible novel therapeutic and diagnostic interventions in asthma. ..
  51. Mechanism of Arginine Transport in Cardiac Myocytes
    RUBEN PELUFFO; Fiscal Year: 2009
    ..Altogether, these studies will provide a detailed picture of the molecular events that take place during cationic amino acid transport into cells and how regulatory mechanisms may alter transport function. ..
  52. ABERRANT TRANSCRIPTS IN AGE RELATED NEURAL DISORDERS
    Chien Liang Lin; Fiscal Year: 1999
    ..abstract_text> ..
  53. Dynamic regulation of inhibitory synaptic transmission through GABA metabolism
    GREGORY MATHEWS; Fiscal Year: 2009
    ....
  54. REGULATION OF AMINO ACID TRANSPORT IN ERYTHROID CELLS
    JAYDUTT VADGAMA; Fiscal Year: 1990
    ..We will first identify and characterize the various amino acid transport systems which may be expressed at specific stages of development...
  55. RECEPTOR AND TRANSPORTER REGULATION OF NO SYNTHASES
    RICHARD VENEMA; Fiscal Year: 2002
    ..Finally, we will test the hypothesis that the iNOS-CAT2 interaction facilitates NO release from vascular smooth muscle cells and will define the domains in the two proteins that are involved in the interaction. ..
  56. Microarray Discovery of Hepatic Biomarkers
    Bonnie Lau; Fiscal Year: 2009
    ..It is predicted that genes involved in oxidant and metabolic stress responses, such as LAT1, will be central in adaptation following liver injury. ..
  57. RAS CONTROL OF AMINO ACID TRANSPORT IN YEAST
    JANETTA GARRETT; Fiscal Year: 1992
    ..These assays are designed to determine the mechanism of coordination between general signals for cell growth and the transport of nitrogen compounds into the cell. ..
  58. Solution NMR Studies of an ATP-Binding Cassette
    Chunyu Wang; Fiscal Year: 2003
    ..abstract_text> ..
  59. Characterization of a novel endosomal sorting complex
    ERIC SPEAR; Fiscal Year: 2007
    ..unreadable] [unreadable]..
  60. Novel, in Vivo Measures of Rat Spinal Cord Glutamate
    Susan Queen; Fiscal Year: 2007
    ..It will be essential to validate the technique in rat spinal cord prior to application of the method to spinal cord injury. [unreadable] [unreadable]..
  61. AMINO ACID TRANSPORT SYSTEMS IN SKELETAL MUSCLE
    Patricia King; Fiscal Year: 1992
    ..Further scientific data for the CRISP System are unavailable at this time...
  62. Excitatory Amino Acid Transporter Expression and Regulation in Schizophrenia
    Deborah Bauer; Fiscal Year: 2008
    ..Defining the specific abnormalities in glutamatergic neurotransmission may be used to target more effective diagnostic and treatment strategies. [unreadable] [unreadable] [unreadable]..