membrane transport proteins

Summary

Summary: Membrane proteins whose primary function is to facilitate the transport of molecules across a biological membrane. Included in this broad category are proteins involved in active transport (BIOLOGICAL TRANSPORT, ACTIVE), facilitated transport and ION CHANNELS.

Top Publications

  1. ncbi Local, efflux-dependent auxin gradients as a common module for plant organ formation
    Eva Benkova
    Zentrum für Molekularbiologie der Pflanzen, Universitat Tubingen, 72076 Tubingen, Germany
    Cell 115:591-602. 2003
  2. ncbi Efflux-dependent auxin gradients establish the apical-basal axis of Arabidopsis
    Jiri Friml
    Zentrum für Molekularbiologie der Pflanzen, Universitat Tubingen, Auf der Morgenstelle 3, 72076 Tubingen, Germany
    Nature 426:147-53. 2003
  3. ncbi The PIN auxin efflux facilitator network controls growth and patterning in Arabidopsis roots
    Ikram Blilou
    Department of Molecular Genetics, Utrecht University, Padualaan 8, 3584CH Utrecht, The Netherlands
    Nature 433:39-44. 2005
  4. ncbi The twin-arginine translocation (Tat) protein export pathway
    Tracy Palmer
    Division of Molecular Microbiology, College of Life Sciences, University of Dundee, Dundee DD1 5EH, UK
    Nat Rev Microbiol 10:483-96. 2012
  5. pmc The Transporter Classification Database: recent advances
    Milton H Saier
    Division of Biological Sciences and Department of Computer Science and Engineering, University of California at San Diego, La Jolla, CA 92093 0116, USA
    Nucleic Acids Res 37:D274-8. 2009
  6. ncbi The Arabidopsis GNOM ARF-GEF mediates endosomal recycling, auxin transport, and auxin-dependent plant growth
    Niko Geldner
    ZMBP, Entwicklungsgenetik, Universitat Tubingen, Auf der Morgenstelle 3, D 72076, Tubingen, Germany
    Cell 112:219-30. 2003
  7. pmc The Chlamydomonas genome reveals the evolution of key animal and plant functions
    Sabeeha S Merchant
    Department of Chemistry and Biochemistry, University of California at Los Angeles, Los Angeles, CA 90095, USA
    Science 318:245-50. 2007
  8. ncbi Multidrug-resistance efflux pumps - not just for resistance
    Laura J V Piddock
    Antimicrobial Agents Research Group, Division of Immunity and Infection, The Medical School, University of Birmingham, Birmingham B15 2TT, UK
    Nat Rev Microbiol 4:629-36. 2006
  9. ncbi Crystal structure of a bacterial homologue of Na+/Cl--dependent neurotransmitter transporters
    Atsuko Yamashita
    Department of Biochemistry and Molecular Biophysics and
    Nature 437:215-23. 2005
  10. ncbi A class of membrane proteins shaping the tubular endoplasmic reticulum
    Gia K Voeltz
    Howard Hughes Medical Institute and Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
    Cell 124:573-86. 2006

Research Grants

  1. PATHOGENESIS OF CORNEAL EDEMA AFTER INTRAOCULAR SURGERY
    Henry Edelhauser; Fiscal Year: 2006
  2. 2010 and 2012 Membrane Transport Proteins
    Robert H Edwards; Fiscal Year: 2012
  3. Extracellular pH modulation by Candida albicans in vitro and in vivo
    Michael C Lorenz; Fiscal Year: 2013
  4. 2011 Multidrug Efflux Systems GRC
    Olga Lomovskaya; Fiscal Year: 2011
  5. CANDIDACIDAL MECHANISMS OF SALIVARY HISTATINS
    Mira Edgerton; Fiscal Year: 2013
  6. STRUCTURE AND FUNCTION OF THE GLUCOSE TRANSPORTER
    Mike M Mueckler; Fiscal Year: 2010
  7. Structure and Mechanism of a Virtual Proton Pump
    Christopher Miller; Fiscal Year: 2013
  8. Dynamics of the Lactose Permease of Escherichia coli
    HOWARD RONALD KABACK; Fiscal Year: 2013
  9. Novel capture reagents for membrane protein structure determination
    Lan Guan; Fiscal Year: 2013
  10. MECHANISM OF CARBOHYDRATE TRANSPORT IN BACTERIA
    Milton Saier; Fiscal Year: 2000

Detail Information

Publications352 found, 100 shown here

  1. ncbi Local, efflux-dependent auxin gradients as a common module for plant organ formation
    Eva Benkova
    Zentrum für Molekularbiologie der Pflanzen, Universitat Tubingen, 72076 Tubingen, Germany
    Cell 115:591-602. 2003
    ..Our results suggest that PIN-dependent, local auxin gradients represent a common module for formation of all plant organs, regardless of their mature morphology or developmental origin...
  2. ncbi Efflux-dependent auxin gradients establish the apical-basal axis of Arabidopsis
    Jiri Friml
    Zentrum für Molekularbiologie der Pflanzen, Universitat Tubingen, Auf der Morgenstelle 3, 72076 Tubingen, Germany
    Nature 426:147-53. 2003
    ..Our results indicate how the establishment of cell polarity, polar auxin efflux and local auxin response result in apical-basal axis formation of the embryo, and thus determine the axiality of the adult plant...
  3. ncbi The PIN auxin efflux facilitator network controls growth and patterning in Arabidopsis roots
    Ikram Blilou
    Department of Molecular Genetics, Utrecht University, Padualaan 8, 3584CH Utrecht, The Netherlands
    Nature 433:39-44. 2005
    ..Our data reveal an interaction network of auxin transport facilitators and root fate determinants that control patterning and growth of the root primordium...
  4. ncbi The twin-arginine translocation (Tat) protein export pathway
    Tracy Palmer
    Division of Molecular Microbiology, College of Life Sciences, University of Dundee, Dundee DD1 5EH, UK
    Nat Rev Microbiol 10:483-96. 2012
    ..In this Review, we summarize recent advances in our understanding of how this remarkable machine operates...
  5. pmc The Transporter Classification Database: recent advances
    Milton H Saier
    Division of Biological Sciences and Department of Computer Science and Engineering, University of California at San Diego, La Jolla, CA 92093 0116, USA
    Nucleic Acids Res 37:D274-8. 2009
    ..The availability of these tools has resulted in recognition of distant phylogenetic relationships and tremendous expansion of the information available to TCDB users...
  6. ncbi The Arabidopsis GNOM ARF-GEF mediates endosomal recycling, auxin transport, and auxin-dependent plant growth
    Niko Geldner
    ZMBP, Entwicklungsgenetik, Universitat Tubingen, Auf der Morgenstelle 3, D 72076, Tubingen, Germany
    Cell 112:219-30. 2003
    ..Our results demonstrate that GNOM is required for the recycling of auxin transport components and suggest that ARF-GEFs regulate specific endosomal trafficking pathways...
  7. pmc The Chlamydomonas genome reveals the evolution of key animal and plant functions
    Sabeeha S Merchant
    Department of Chemistry and Biochemistry, University of California at Los Angeles, Los Angeles, CA 90095, USA
    Science 318:245-50. 2007
    ....
  8. ncbi Multidrug-resistance efflux pumps - not just for resistance
    Laura J V Piddock
    Antimicrobial Agents Research Group, Division of Immunity and Infection, The Medical School, University of Birmingham, Birmingham B15 2TT, UK
    Nat Rev Microbiol 4:629-36. 2006
    ..Here, I present the accumulating evidence that multidrug-resistance efflux pumps have roles in bacterial pathogenicity and propose that these pumps therefore have greater clinical relevance than is usually attributed to them...
  9. ncbi Crystal structure of a bacterial homologue of Na+/Cl--dependent neurotransmitter transporters
    Atsuko Yamashita
    Department of Biochemistry and Molecular Biophysics and
    Nature 437:215-23. 2005
    ..The structure reveals the architecture of this important class of transporter, illuminates the determinants of substrate binding and ion selectivity, and defines the external and internal gates...
  10. ncbi A class of membrane proteins shaping the tubular endoplasmic reticulum
    Gia K Voeltz
    Howard Hughes Medical Institute and Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
    Cell 124:573-86. 2006
    ..The simultaneous absence of the reticulons and Yop1p in S. cerevisiae results in disrupted tubular ER. We propose that these "morphogenic" proteins partition into and stabilize highly curved ER membrane tubules...
  11. ncbi Protein translocation across the bacterial cytoplasmic membrane
    Arnold J M Driessen
    Department of Molecular Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute and the Zernike Institute for Advanced Materials, University of Groningen, Haren, The Netherlands
    Annu Rev Biochem 77:643-67. 2008
    ..This review summarizes the present knowledge of the mechanism and structure of the Sec translocase, with a special emphasis on unresolved questions and topics of current research...
  12. ncbi Auxin transport inhibitors block PIN1 cycling and vesicle trafficking
    N Geldner
    , , Auf der Morgenstelle 3, , Germany
    Nature 413:425-8. 2001
    ..In support of our conclusion, the vesicle-trafficking inhibitor brefeldin A mimics physiological effects of auxin transport inhibitors...
  13. pmc Molecular basis of alternating access membrane transport by the sodium-hydantoin transporter Mhp1
    Tatsuro Shimamura
    Division of Molecular Biosciences, Membrane Protein Crystallography Group, Imperial College, London SW7 2AZ, UK
    Science 328:470-3. 2010
    ..This forms the basis of an alternating access mechanism applicable to many transporters of this emerging superfamily...
  14. ncbi AtPIN4 mediates sink-driven auxin gradients and root patterning in Arabidopsis
    Jiri Friml
    Max Delbruck Laboratorium in der Max Planck Gesellschaft, Koln, Germany
    Cell 108:661-73. 2002
    ..We propose a role for AtPIN4 in generating a sink for auxin below the quiescent center of the root meristem that is essential for auxin distribution and patterning...
  15. ncbi The structural basis of secondary active transport mechanisms
    Lucy R Forrest
    Structural Biology Department, Max Planck Institute for Biophysics, Frankfurt, Germany
    Biochim Biophys Acta 1807:167-88. 2011
    ....
  16. pmc The major facilitator superfamily (MFS) revisited
    Vamsee S Reddy
    Division of Biological Sciences, University of California at San Diego, La Jolla, CA, USA
    FEBS J 279:2022-35. 2012
    ..Finally, the MFS families with no known function were subjected to genomic context analyses leading to functional predictions...
  17. pmc Clinically relevant chromosomally encoded multidrug resistance efflux pumps in bacteria
    Laura J V Piddock
    Antimicrobial Agents Research Group, Division of Immunity and Infection, The Medical School, University of Birmingham, Birmingham, United Kingdom, B15 2TT
    Clin Microbiol Rev 19:382-402. 2006
    ..Inhibitors of various efflux pump systems have been described; typically these are plant alkaloids, but as yet no product has been marketed...
  18. ncbi PIN proteins perform a rate-limiting function in cellular auxin efflux
    Jan Petrasek
    Institute of Experimental Botany, The Academy of Sciences of the Czech Republic, 165 02 Prague 6, Czech Republic
    Science 312:914-8. 2006
    ..This suggests a direct involvement of PINs in catalyzing cellular auxin efflux...
  19. pmc TransportDB: a comprehensive database resource for cytoplasmic membrane transport systems and outer membrane channels
    Qinghu Ren
    The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850, USA
    Nucleic Acids Res 35:D274-9. 2007
    ..TransportDB has now been in existence for over 10 years and continues to be regularly updated with new evidence and data from newly sequenced genomes, as well as having new features added periodically...
  20. ncbi Subcellular homeostasis of phytohormone auxin is mediated by the ER-localized PIN5 transporter
    Jozef Mravec
    Department of Plant Systems Biology, VIB and Department of Plant Biotechnology and Genetics, Ghent University, 9052 Gent, Belgium
    Nature 459:1136-40. 2009
    ....
  21. pmc ABP1 mediates auxin inhibition of clathrin-dependent endocytosis in Arabidopsis
    Stephanie Robert
    Department of Plant Systems Biology, VIB, 9052 Gent, Belgium
    Cell 143:111-21. 2010
    ....
  22. ncbi Polar PIN localization directs auxin flow in plants
    Justyna Wisniewska
    Center for Plant Molecular Biology ZMBP, Tubingen University, D 72076 Tubingen, Germany
    Science 312:883. 2006
    ..This finding provides a crucial piece in the puzzle of how auxin flow can be redirected via rapid changes in PIN polarity...
  23. pmc Control of leaf vascular patterning by polar auxin transport
    Enrico Scarpella
    Department of Biological Sciences, University of Alberta, Edmonton, Alberta T6G 2E9, Canada
    Genes Dev 20:1015-27. 2006
    ..We derive a model for self-regulated, reiterative patterning of all vein orders and postulate at its onset a common epidermal auxin-focusing mechanism for major-vein positioning and phyllotactic patterning...
  24. pmc Structural mechanism of abscisic acid binding and signaling by dimeric PYR1
    Noriyuki Nishimura
    Division of Biological Sciences, Cell and Developmental Biology Section, University of California at San Diego, La Jolla, CA 92093, USA
    Science 326:1373-9. 2009
    ..Site-directed PYR1 mutants designed to disrupt hormone binding lose ABA-triggered interactions with type 2C protein phosphatase partners in planta...
  25. ncbi Auxin transport routes in plant development
    Jan Petrasek
    Institute of Experimental Botany, ASCR, Prague 6, Czech Republic
    Development 136:2675-88. 2009
    ..This activity crucially contributes to the correct setting of developmental cues in embryogenesis, organogenesis, vascular tissue formation and directional growth in response to environmental stimuli...
  26. pmc The neuronal sortilin-related receptor SORL1 is genetically associated with Alzheimer disease
    Ekaterina Rogaeva
    Centre for Research in Neurodegenerative Diseases, Department of Medicine, Department, University of Toronto, Toronto, Ontario, Canada
    Nat Genet 39:168-77. 2007
    ..These data suggest that inherited or acquired changes in SORL1 expression or function are mechanistically involved in causing Alzheimer disease...
  27. ncbi Regulation of polar auxin transport by AtPIN1 in Arabidopsis vascular tissue
    L Gälweiler
    Max Delbruck Laboratorium in der Max Planck Gesellschaft, Carl von Linne Weg 10, D 50829 Köln, Germany
    Science 282:2226-30. 1998
    ..AtPIN1 may act as a transmembrane component of the auxin efflux carrier...
  28. ncbi A genetic framework for the control of cell division and differentiation in the root meristem
    Raffaele Dello Ioio
    Dipartimento di Genetica e Biologia Molecolare, Laboratorio di Genomica e Proteomica Funzionale dei Sistemi Modello FGPL, Università La Sapienza Piazzale Aldo Moro 5, 00185 Rome, Italy
    Science 322:1380-4. 2008
    ....
  29. pmc The ectodomain of Toll-like receptor 9 is cleaved to generate a functional receptor
    Sarah E Ewald
    Division of Immunology and Pathogenesis, Department of Molecular and Cell Biology, University of California, Berkeley, 405 Life Sciences Addition, Berkeley, California 94720 3200, USA
    Nature 456:658-62. 2008
    ..We propose that ectodomain cleavage represents a strategy to restrict receptor activation to endolysosomal compartments and prevent TLRs from responding to self nucleic acids...
  30. pmc Chloroquine resistance before and after its withdrawal in Kenya
    Leah Mwai
    Kenya Medical Research Institute Wellcome Trust Collaborative Research Programme, Kilifi, Kenya
    Malar J 8:106. 2009
    ..Changes to those that occurred in the dihydrofolate reductase gene (dhfr) that confers resistance to the replacement drug, pyrimethamine/sulphadoxine were also compared...
  31. pmc Neuronal sorting protein-related receptor sorLA/LR11 regulates processing of the amyloid precursor protein
    Olav M Andersen
    Max Delbrueck Center for Molecular Medicine, D 13125 Berlin, Germany
    Proc Natl Acad Sci U S A 102:13461-6. 2005
    ..Consequently, reduced receptor expression in the human brain may increase Abeta production and plaque formation and promote spontaneous AD...
  32. ncbi Crystal structures of a multidrug transporter reveal a functionally rotating mechanism
    Satoshi Murakami
    Department of Cell Membrane Biology, Institute of Scientific and Industrial Research, Osaka University, Ibaraki, Osaka 567 0047, Japan
    Nature 443:173-9. 2006
    ..The voluminous binding pocket is aromatic and allows multi-site binding. The structures indicate that drugs are exported by a three-step functionally rotating mechanism in which substrates undergo ordered binding change...
  33. ncbi Functional redundancy of PIN proteins is accompanied by auxin-dependent cross-regulation of PIN expression
    Anne Vieten
    Centre for Molecular Biology of Plants, University Tübingen, Auf der Morgenstelle 3, 72076 Tubingen, Germany
    Development 132:4521-31. 2005
    ..The compensatory properties of the PIN-dependent transport network might enable the stabilization of auxin gradients and potentially contribute to the robustness of plant adaptive development...
  34. pmc AtPIN2 defines a locus of Arabidopsis for root gravitropism control
    A Muller
    Max Delbruck Laboratorium in der Max Planck Gesellschaft, Max Planck Institut fur Zuchtungsforschung, Carl von Linne Weg 10, D 50829 Köln, Germany
    EMBO J 17:6903-11. 1998
    ..These results suggest that AtPIN2 plays an important role in control of gravitropism regulating the redistribution of auxin from the stele towards the elongation zone of roots...
  35. pmc Generation of cell polarity in plants links endocytosis, auxin distribution and cell fate decisions
    Pankaj Dhonukshe
    Department of Biology, Faculty of Science, Utrecht University, Padualaan 8, 3584 CH, Utrecht, The Netherlands
    Nature 456:962-6. 2008
    ..It also highlights the link between endocytosis-dependent polarity of individual cells and auxin distribution-dependent cell fate establishment for multicellular patterning...
  36. pmc In vivo selection of Plasmodium falciparum parasites carrying the chloroquine-susceptible pfcrt K76 allele after treatment with artemether-lumefantrine in Africa
    Christin Sisowath
    Infectious Diseases Unit, Department of Medicine and Karolinska Institutet, Stockholm, Sweden
    J Infect Dis 199:750-7. 2009
    ..Identification of drug-specific parasite determinants that contribute to treatment failures will provide important tools for the detection and surveillance of AL resistance...
  37. pmc In vitro activities of piperaquine, lumefantrine, and dihydroartemisinin in Kenyan Plasmodium falciparum isolates and polymorphisms in pfcrt and pfmdr1
    Leah Mwai
    Kenya Medical Research Institute KEMRI Wellcome Trust Collaborative Research Program, Kilifi, Kenya
    Antimicrob Agents Chemother 53:5069-73. 2009
    ..Therefore, the use of LM-artemether is likely to lead to the selection of more CQ-susceptible parasites...
  38. ncbi Crystal structure of bacterial multidrug efflux transporter AcrB
    Satoshi Murakami
    Department of Cell Membrane Biology, Institute of Scientific and Industrial Research, Osaka University, Ibaraki, Osaka 567 0047, Japan
    Nature 419:587-93. 2002
    ....
  39. ncbi Structure, function and inhibition of RND efflux pumps in Gram-negative bacteria: an update
    Jessica M A Blair
    Antimicrobial Agents Research Group, School of Immunity and Infection, College of Medical and Dental Sciences, University of Birmingham, Birmingham B15 2TT, UK
    Curr Opin Microbiol 12:512-9. 2009
    ..The importance of these systems in both MDR and pathogenicity has made RND systems the target of new drugs aimed at inhibiting their function. The wealth of new structural and functional data will inform rational drug design...
  40. ncbi Skin pigmentation, biogeographical ancestry and admixture mapping
    Mark D Shriver
    Department of Anthropology, Penn State University, 409 Carpenter Bld, University Park, PA 16802, USA
    Hum Genet 112:387-99. 2003
    ..The implications and applications of ancestry estimates in biomedical research are discussed...
  41. pmc Genome sequence of the human malaria parasite Plasmodium falciparum
    Malcolm J Gardner
    The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, Maryland 20850, USA
    Nature 419:498-511. 2002
    ..The genome sequence provides the foundation for future studies of this organism, and is being exploited in the search for new drugs and vaccines to fight malaria...
  42. ncbi A regulated auxin minimum is required for seed dispersal in Arabidopsis
    Karim Sorefan
    Crop Genetics Department, John Innes Centre, Norwich Research Park, Colney Lane, Norwich, Norfolk NR4 7UH, UK
    Nature 459:583-6. 2009
    ..We propose that the simplicity of formation and maintenance make local hormone minima particularly well suited to specify a small number of cells such as the stripes at the valve margins...
  43. ncbi Nitrate transporters and peptide transporters
    Yi Fang Tsay
    Institute of Molecular Biology, Academia Sinica, Taipei, Taiwan
    FEBS Lett 581:2290-300. 2007
    ..In higher plants, there is another family of peptide transporters, called oligopeptide transporters (OPTs), which transport tetra/pentapeptides. In addition, some OPTs transport GSH, GSSH, GSH conjugates, phytochelatins, and metals...
  44. ncbi Return of chloroquine antimalarial efficacy in Malawi
    Miriam K Laufer
    University of Maryland School of Medicine, Baltimore, MD 21201, USA
    N Engl J Med 355:1959-66. 2006
    ..The molecular marker of chloroquine-resistant falciparum malaria subsequently declined in prevalence and was undetectable by 2001, suggesting that chloroquine might once again be effective in Malawi...
  45. ncbi Blue eye color in humans may be caused by a perfectly associated founder mutation in a regulatory element located within the HERC2 gene inhibiting OCA2 expression
    Hans Eiberg
    Department of Cellular and Molecular Medicine, Section IV Build 24 4, Panum Institute, University of Copenhagen, Blegdamsvej 3b, 2200, Copenhagen, Denmark
    Hum Genet 123:177-87. 2008
    ..In addition, an LOD score of Z = 4.21 between hair color and D14S72 was obtained in the large family, indicating that RABGGTA is a candidate gene for hair color...
  46. pmc Chloroquine resistance in Plasmodium falciparum malaria parasites conferred by pfcrt mutations
    Amar bir Singh Sidhu
    Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Science 298:210-3. 2002
    ..pfcrt mutations increased susceptibility to artemisinin and quinine and minimally affected amodiaquine activity; hence, these antimalarials warrant further investigation as agents to control chloroquine-resistant falciparum malaria...
  47. pmc Structure of the TatC core of the twin-arginine protein transport system
    Sarah E Rollauer
    Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK
    Nature 492:210-4. 2012
    ..This structure provides a molecular description of the core of the Tat translocation system and a framework for understanding the unique Tat transport mechanism...
  48. ncbi Niemann-Pick C1 Like 1 protein is critical for intestinal cholesterol absorption
    Scott W Altmann
    Department of Cardiovascular Endocrine Research, Schering Plough Research Institute, 2015 Galloping Hill Road, Kenilworth, NJ, 07033 0539, USA
    Science 303:1201-4. 2004
    ..Ezetimibe, a drug that inhibits cholesterol absorption, had no effect in NPC1L1 knockout mice, suggesting that NPC1L1 resides in an ezetimibe-sensitive pathway responsible for intestinal cholesterol absorption...
  49. pmc Auxin transporters--why so many?
    Eva Zazimalova
    Institute of Experimental Botany AS CR, Rozvojova 263, CZ 165 02 Prague 6, Czech Republic
    Cold Spring Harb Perspect Biol 2:a001552. 2010
    ....
  50. pmc Manganese transporters Yfe and MntH are Fur-regulated and important for the virulence of Yersinia pestis
    Robert D Perry
    Department of Microbiology, Immunology, and Molecular Genetics, University of Kentucky, Lexington, KY 40536 0298, USA
    Microbiology 158:804-15. 2012
    ..This suggests that Mn availability, bacterial Mn requirements or Mn transporters used by Y. pestis are different in the lungs (pneumonic plague) compared with systemic disease...
  51. pmc The Escherichia coli MntR miniregulon includes genes encoding a small protein and an efflux pump required for manganese homeostasis
    Lauren S Waters
    Cell Biology and Metabolism Program, Eunice Kennedy Shriver National Institute of Child Health and Human Development, Bethesda, Maryland, USA
    J Bacteriol 193:5887-97. 2011
    ..Deletion of mntP leads to profound manganese sensitivity and to elevated intracellular manganese levels. This work thus defines two new proteins involved in manganese homeostasis and suggests mechanisms for their action...
  52. pmc Geographic patterns of Plasmodium falciparum drug resistance distinguished by differential responses to amodiaquine and chloroquine
    Juliana Martha Sá
    Laboratory of Malaria and Vector Research and Biostatistics Research Branch, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 106:18883-9. 2009
    ..Increasing use of AQ in Africa poses the threat of a selective sweep of highly AQ-resistant, CQ-resistant parasites with pfcrt and pfmdr1 mutations that are as advantaged and persistent as in South America...
  53. ncbi A PINOID-dependent binary switch in apical-basal PIN polar targeting directs auxin efflux
    Jiri Friml
    Developmental Genetics, Center for Molecular Biology of Plants, University Tübingen, Auf der Morgenstelle 3, D 72076 Tubingen, Germany
    Science 306:862-5. 2004
    ..Our results show that a PINOID-dependent binary switch controls PIN polarity and mediates changes in auxin flow to create local gradients for patterning processes...
  54. pmc Three genome-wide association studies and a linkage analysis identify HERC2 as a human iris color gene
    Manfred Kayser
    Department of Forensic Molecular Biology, Erasmus University Medical Center, 3000 CA Rotterdam, The Netherlands
    Am J Hum Genet 82:411-23. 2008
    ..Testing markers in the HERC2-OCA2 region may be useful in forensic applications to predict eye color phenotypes of unknown persons of European genetic origin...
  55. ncbi Genetic evidence for the convergent evolution of light skin in Europeans and East Asians
    Heather L Norton
    Department of Anthropology, The Pennsylvania State University, PA, USA
    Mol Biol Evol 24:710-22. 2007
    ..These findings support a case for the recent convergent evolution of a lighter pigmentation phenotype in Europeans and East Asians...
  56. pmc In vitro sensitivities of Plasmodium falciparum to different antimalarial drugs in Uganda
    Samuel L Nsobya
    Department of Medicine, Makerere University, Kampala, Uganda
    Antimicrob Agents Chemother 54:1200-6. 2010
    ..In summary, we demonstrated in parasites from Kampala a range of sensitivities to older drugs; correlation of sensitivities to CQ, MDAQ, and QN; and good activity against nearly all strains for DHA, LM, and PQ...
  57. ncbi Mitofusin-1 protein is a generally expressed mediator of mitochondrial fusion in mammalian cells
    Ansgar Santel
    Department of Developmental Biology, Stanford University School of Medicine, Stanford, CA 94305, USA
    J Cell Sci 116:2763-74. 2003
    ..Thus, Mfn1 appears to be a key player in mediating mitochondrial fusion and morphology in mammalian cells...
  58. ncbi The SAT1 flipper, an optimized tool for gene disruption in Candida albicans
    Oliver Reuss
    Institut für Molekulare Infektionsbiologie, Universitat Wurzburg, Rontgenring 11, D 97070 Wurzburg, Germany
    Gene 341:119-27. 2004
    ..The SAT1 flipping method provides a highly efficient method for gene disruption in C. albicans wild-type strains, which eliminates currently encountered problems in the genetic analysis of this important human fungal pathogen...
  59. ncbi Molecular mechanisms of iron uptake in fungi
    Daniel J Kosman
    Department of Biochemistry, 140 Farber Hall, The University at Buffalo, School of Medicine and Biomedical Sciences, Buffalo, NY 14214, USA
    Mol Microbiol 47:1185-97. 2003
    ..The integration of these multiple uptake mechanisms and their regulation into over-all iron homeostasis in yeast concludes this brief review...
  60. pmc Control of intracellular heme levels: heme transporters and heme oxygenases
    Anwar A Khan
    Department of Medicine, Section of Hematology Oncology, University of Illinois College of Medicine, 909 South Wolcott Avenue, Chicago, IL 0612, USA
    Biochim Biophys Acta 1813:668-82. 2011
    ..This article is part of a Special Issue entitled: 11th European Symposium on Calcium...
  61. pmc A single SNP in an evolutionary conserved region within intron 86 of the HERC2 gene determines human blue-brown eye color
    Richard A Sturm
    Institute for Molecular Bioscience, University of Queensland, Brisbane QLD 4072, Australia
    Am J Hum Genet 82:424-31. 2008
    ....
  62. pmc Hyperactivation of oxidative mitochondrial metabolism in epithelial cancer cells in situ: visualizing the therapeutic effects of metformin in tumor tissue
    Diana Whitaker-Menezes
    The Jefferson Stem Cell Biology and Regenerative Medicine Center, Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, PA, USA
    Cell Cycle 10:4047-64. 2011
    ..Thus, we believe that mitochondria are both the "powerhouse" and "Achilles' heel" of cancer cells...
  63. pmc Alignment between PIN1 polarity and microtubule orientation in the shoot apical meristem reveals a tight coupling between morphogenesis and auxin transport
    Marcus G Heisler
    Division of Biology, California Institute of Technology, Pasadena, California, United States of America
    PLoS Biol 8:e1000516. 2010
    ..Lastly, through mathematical modeling we show that such a biophysical coupling could mediate the feedback loop between auxin and its transport that underlies plant phyllotaxis...
  64. ncbi The auxin influx carrier LAX3 promotes lateral root emergence
    Kamal Swarup
    School of Biosciences and Centre for Plant Integrative Biology, University of Nottingham, LE12 5RD, UK
    Nat Cell Biol 10:946-54. 2008
    ..Increased LAX3 activity reinforces the auxin-dependent induction of a selection of cell-wall-remodelling enzymes, which are likely to promote cell separation in advance of developing lateral root primordia...
  65. ncbi The Unc93b1 mutation 3d disrupts exogenous antigen presentation and signaling via Toll-like receptors 3, 7 and 9
    Koichi Tabeta
    Department of Immunology, The Scripps Research Institute, La Jolla, California 92037, USA
    Nat Immunol 7:156-64. 2006
    ..Innate responses to nucleic acids and exogenous antigen presentation, which both initiate in endosomes, thus seem to depend on an endoplasmic reticulum-resident protein, which suggests communication between these organellar systems...
  66. ncbi Antagonistic regulation of PIN phosphorylation by PP2A and PINOID directs auxin flux
    Marta Michniewicz
    Zentrum für Molekularbiologie der Pflanzen, Universitat Tubingen, Tubingen, Germany
    Cell 130:1044-56. 2007
    ..In the case of PIN proteins, this mechanism enables switches in the direction of intercellular auxin fluxes, which mediate differential growth, tissue patterning, and organogenesis...
  67. pmc Loss of KCNJ10 protein expression abolishes endocochlear potential and causes deafness in Pendred syndrome mouse model
    Philine Wangemann
    Anatomy and Physiology Department, Kansas State University, Manhattan, Kansas, USA
    BMC Med 2:30. 2004
    ..We investigated the relationship between pendrin and deafness using mice that have (Slc26a4+/+) or lack a complete Slc26a4 gene (Slc26a4-/-)...
  68. ncbi Multidrug resistance proteins: role of P-glycoprotein, MRP1, MRP2, and BCRP (ABCG2) in tissue defense
    Elaine M Leslie
    Division of Drug Delivery and Disposition, School of Pharmacy, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    Toxicol Appl Pharmacol 204:216-37. 2005
    ..Species variations in substrate specificity and tissue distribution of these transporters are also addressed since these properties have implications for in vivo models of toxicity used for drug discovery and development...
  69. pmc EIR1, a root-specific protein involved in auxin transport, is required for gravitropism in Arabidopsis thaliana
    C Luschnig
    Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142 1479 USA
    Genes Dev 12:2175-87. 1998
    ..Expression of the EIR1 gene in Saccharomyces cerevisiae confers resistance to fluorinated indolic compounds. Taken together, these data suggest that the EIR1 protein has a root-specific role in the transport of auxin...
  70. pmc The PIN-FORMED (PIN) protein family of auxin transporters
    Pavel Krecek
    Institute of Experimental Botany AS CR, Rozvojova 263, CZ 16502 Prague 6, Czech Republic
    Genome Biol 10:249. 2009
    ..Furthermore, the evolution of PIN-based transport, PIN protein structure and more detailed biochemical characterization of the transport function are important topics for further studies...
  71. pmc An auxin-driven polarized transport model for phyllotaxis
    Henrik Jonsson
    Computational Biology and Biological Physics Group, Department of Theoretical Physics, Lund University, S 221 00 Lund, Sweden
    Proc Natl Acad Sci U S A 103:1633-8. 2006
    ..The model shows how polarized transport can drive the formation of regular patterns...
  72. ncbi The ubiquitin code of yeast permease trafficking
    Elsa Lauwers
    Physiologie Moléculaire de la Cellule, Institut de Biologie et de Médecine Moléculaires IBMM, Universite Libre de Bruxelles ULB, Gosselies, Belgium
    Trends Cell Biol 20:196-204. 2010
    ..In particular, K63-linked ubiquitin chains now emerge as a specific signal for protein sorting into the MVB pathway. A complete view of the ubiquitin code governing yeast permease trafficking might not be far off...
  73. pmc A genomewide association study of skin pigmentation in a South Asian population
    Renee P Stokowski
    Perlegen Sciences, Mountain View, CA 94043, USA
    Am J Hum Genet 81:1119-32. 2007
    ..Our study is the first to interrogate polymorphisms across the genome, to find genetic determinants of the natural variation of skin pigmentation within a human population...
  74. ncbi Coordinated polar localization of auxin efflux carrier PIN1 by GNOM ARF GEF
    T Steinmann
    Entwicklungsgenetik, Zentrum für Molekularbiologie der Pflanzen, Universitat Tubingen, Auf der Morgenstelle 1, D 72076 Tubingen, Germany
    Science 286:316-8. 1999
    ..GNOM is a membrane-associated guanine-nucleotide exchange factor on ADP-ribosylation factor G protein (ARF GEF). Thus, GNOM-dependent vesicle trafficking may establish cell polarity, resulting in polar auxin transport...
  75. ncbi The PIN auxin efflux facilitators: evolutionary and functional perspectives
    Ivan A Paponov
    Institut fur Biologie II, Universitat Freiburg, Schanzlestrasse 1, 79104 Freiburg, Germany
    Trends Plant Sci 10:170-7. 2005
    ..Here, we present an overview of this family of auxin efflux facilitators in monocot and dicot plants, summarizing their evolutionary history, expression profiles and, where appropriate, relating them to protein function...
  76. ncbi Combined prediction of Tat and Sec signal peptides with hidden Markov models
    Pantelis G Bagos
    Department of Computer Science and Biomedical Informatics, University of Central Greece, Papasiopoulou 2 4, Lamia 35100, Greece
    Bioinformatics 26:2811-7. 2010
    ..The purpose of this work is to develop a novel method for predicting and discriminating Sec from Tat signal peptides at better accuracy...
  77. ncbi Strigolactones enhance competition between shoot branches by dampening auxin transport
    Scott Crawford
    Department of Biology, University of York, York, UK
    Development 137:2905-13. 2010
    ....
  78. ncbi Mutations in the gene encoding B, a novel transporter protein, reduce melanin content in medaka
    S Fukamachi
    Department of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo, Kashiwa no ha 5 1 5, Kashiwa shi, Chiba ken 277 0882, Japan
    Nat Genet 28:381-5. 2001
    ..Analysis of AIM1 orthologs should provide new insights into the regulation of melanogenesis in both teleosts and mammals...
  79. pmc Structural analysis of a monomeric form of the twin-arginine leader peptide binding chaperone Escherichia coli DmsD
    Charles M Stevens
    Department of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, British Columbia, Canada
    J Mol Biol 389:124-33. 2009
    ..coli DmsA and the most conserved regions on the surface of EcDmsD...
  80. ncbi Uncoupling protein-2 is critical for nigral dopamine cell survival in a mouse model of Parkinson's disease
    Zane B Andrews
    Department of Obstetrics, Gynecology, and Reproductive Sciences, Yale University School of Medicine, New Haven, Connecticut 06520, USA
    J Neurosci 25:184-91. 2005
    ..The present results expose the critical importance of UCP2 in normal nigral dopamine cell metabolism and offer a novel therapeutic target, UCP2, for the prevention/treatment of Parkinson's disease...
  81. ncbi The expression of vesicular glutamate transporters defines two classes of excitatory synapse
    R T Fremeau
    Department of Neurology, Graduate Program in Neuroscience, UCSF School of Medicine, San Francisco, CA 94143, USA
    Neuron 31:247-60. 2001
    ..Like VGLUT1, this protein localizes to synaptic vesicles and functions as a vesicular glutamate transporter (VGLUT2). The complementary expression of VGLUT1 and 2 defines two distinct classes of excitatory synapse...
  82. pmc Identification and characterization of inhibitors of multidrug resistance efflux pumps in Pseudomonas aeruginosa: novel agents for combination therapy
    O Lomovskaya
    Microcide Pharmaceuticals, Inc, Mountain View, California 94043, USA
    Antimicrob Agents Chemother 45:105-16. 2001
    ..aeruginosa strains that are highly resistant to fluoroquinolones...
  83. pmc Disruption of OsYSL15 leads to iron inefficiency in rice plants
    Sichul Lee
    Department of Integrative Bioscience and Biotechnology, Pohang University of Science and Technology, Pohang 790 784, Republic of Korea
    Plant Physiol 150:786-800. 2009
    ..Overexpression of OsYSL15 increased the Fe concentration in leaves and seeds from transgenic plants. Altogether, these results demonstrate roles for OsYSL15 in Fe uptake and distribution in rice plants...
  84. pmc Structural analysis of substrate binding by the TatBC component of the twin-arginine protein transport system
    Michael J Tarry
    Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, United Kingdom
    Proc Natl Acad Sci U S A 106:13284-9. 2009
    ..Similar TatBC-substrate complexes can be generated by an alternative in vitro reconstitution method and using a different substrate protein...
  85. pmc Meta-analysis of the association between variants in SORL1 and Alzheimer disease
    Christiane Reitz
    The Taub Institute for Research on Alzheimer s Disease and the Aging Brain and the Gertrude H Sergievsky Center, College of Physicians and Surgeons, Columbia University, New York, NY 10032, USA
    Arch Neurol 68:99-106. 2011
    ..To reexamine the association between the neuronal sortilin-related receptor gene (SORL1) and Alzheimer disease (AD)...
  86. pmc CmeABC functions as a multidrug efflux system in Campylobacter jejuni
    Jun Lin
    Food Animal Health Research Program, Department of Veterinary Preventive Medicine, The Ohio State University, Wooster, Ohio 44691, USA
    Antimicrob Agents Chemother 46:2124-31. 2002
    ..Together, these findings formally establish that CmeABC functions as a tripartite multidrug efflux pump that contributes to the intrinsic resistance of C. jejuni to a broad range of structurally unrelated antimicrobial agents...
  87. pmc Constitutive expression of Escherichia coli tat genes indicates an important role for the twin-arginine translocase during aerobic and anaerobic growth
    R L Jack
    Centre for Metalloprotein Spectroscopy and Biology, School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, United Kingdom
    J Bacteriol 183:1801-4. 2001
    ..Expression studies indicate that the tatABCD and tatE transcription units are expressed constitutively. Translational fusion experiments suggest that TatA is synthesized at a much higher level than the other Tat proteins...
  88. ncbi Monitoring of in vitro susceptibilities and molecular markers of resistance of Plasmodium falciparum isolates from Thai-Myanmar border to chloroquine, quinine, mefloquine and artesunate
    Wanna Chaijaroenkul
    Graduate Program in Biomedical Sciences, Faculty of Allied Health Sciences, Thammasat University, Rangsit, Patumthani 12121, Thailand
    Acta Trop 113:190-4. 2010
    ..Moreover, the correlation between pfmdr1 gene amplification and susceptibility of the parasite to MQ, QN and AS was observed (decreased susceptibilities to MQ, QN and AS in isolates with increased pfmdr1 copy number)...
  89. ncbi Mefloquine resistance in Plasmodium falciparum and increased pfmdr1 gene copy number
    Ric N Price
    Department of Cellular and Molecular Medicine Infectious Diseases, St George s Hospital Medical School, London, UK
    Lancet 364:438-47. 2004
    ..A combination of artesunate with mefloquine now cures more than 95% of acute infections. For both treatment regimens, the underlying mechanisms of resistance are not known...
  90. pmc Ins and outs of major facilitator superfamily antiporters
    Christopher J Law
    The Helen L and Martin S Kimmel Center for Biology and Medicine at the Skirball Institute of Biomolecular Medicine, New York University School of Medicine, New York, NY 10016, USA
    Annu Rev Microbiol 62:289-305. 2008
    ..Salt-bridge formation and breakage are involved in the conformational changes of the protein during transport. In this review, we attempt to give an account of a set of mechanistic principles that characterize all MFS antiporters...
  91. pmc The TatA component of the twin-arginine protein transport system forms channel complexes of variable diameter
    Ulrich Gohlke
    Institute of Structural Molecular Biology, School of Crystallography, Birkbeck College, Malet Street, London WC1E 7HX, United Kingdom
    Proc Natl Acad Sci U S A 102:10482-6. 2005
    ..Such dynamic close packing would provide a mechanism to maintain the membrane permeability barrier during transport...
  92. pmc The E Block motif is associated with Legionella pneumophila translocated substrates
    Li Huang
    Department of Molecular Biology and Microbiology, Tufts University School of Medicine, 150 Harrison Avenue, Boston, MA 02111, USA
    Cell Microbiol 13:227-45. 2011
    ..These studies indicate that by analysing subsets of translocated substrates, patterns can be found that allow predictions of important motifs recognized by Icm/Dot...
  93. ncbi Mitochondrial superoxide: production, biological effects, and activation of uncoupling proteins
    Martin D Brand
    MRC Dunn Human Nutrition Unit, Cambridge CB2 2XY, UK
    Free Radic Biol Med 37:755-67. 2004
    ..More recently evolved roles of uncoupling proteins are in adaptive thermogenesis (UCP1) and perhaps as part of a signaling pathway to regulate insulin secretion in pancreatic beta cells (UCP2)...
  94. pmc In vitro sensitivity of Plasmodium falciparum clinical isolates from the China-Myanmar border area to quinine and association with polymorphism in the Na+/H+ exchanger
    Hao Meng
    Parasitology Department, School of Pharmaceutical Sciences, Kunming Medical University, 1168 West Chunrong Road, Kunming, Yunnan Province 650500, China
    Antimicrob Agents Chemother 54:4306-13. 2010
    ..This study provided further support for the importance of pfnhe1 polymorphisms in influencing QNR in P. falciparum...
  95. ncbi Loss of apolipoprotein E receptor LR11 in Alzheimer disease
    Clemens R Scherzer
    Department of Neurology, Emory University, Atlanta, GA, USA
    Arch Neurol 61:1200-5. 2004
    ..Genetic, epidemiologic, and biochemical evidence suggests that apolipoprotein E, low-density lipoprotein receptors, and lipid metabolism play important roles in sporadic Alzheimer disease (AD)...
  96. pmc Hereditary spastic paraplegia proteins REEP1, spastin, and atlastin-1 coordinate microtubule interactions with the tubular ER network
    Seong H Park
    Cellular Neurology Unit, Neurogenetics Branch, National Institute of Neurological Disorders and Stroke NINDS, NIH, Bethesda, Maryland 20892 3738, USA
    J Clin Invest 120:1097-110. 2010
    ..Thus, defects in tubular ER shaping and network interactions with the microtubule cytoskeleton seem to be the predominant pathogenic mechanism of HSP...
  97. pmc Identification of a mutant PfCRT-mediated chloroquine tolerance phenotype in Plasmodium falciparum
    Stephanie G Valderramos
    Department of Microbiology and Immunology, Columbia University Medical Center, New York, New York, United States of America
    PLoS Pathog 6:e1000887. 2010
    ....
  98. ncbi Structure of a complex of the ATPase SecA and the protein-translocation channel
    Jochen Zimmer
    Howard Hughes Medical Institute and Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, Massachusetts 02115, USA
    Nature 455:936-43. 2008
    ..SecA binding generates a 'window' at the lateral gate of the SecY channel and it displaces the plug domain, preparing the channel for signal sequence binding and channel opening...
  99. ncbi Arabidopsis AUX1 gene: a permease-like regulator of root gravitropism
    M J Bennett
    Department of Biological Sciences, University of Warwick, Coventry, CV4 7AL, UK
    Science 273:948-50. 1996
    ..The cloned AUX1 gene can restore the auxin-responsiveness of transgenic aux1 roots. Spatially, AUX1 is expressed in root apical tissues that regulate root gravitropic curvature...
  100. ncbi Vacuolar transporters and their essential role in plant metabolism
    Enrico Martinoia
    Institute of Plant Biology, University of Zurich, Zollikerstr 107, Ch 8008 Zurich, Switzerland
    J Exp Bot 58:83-102. 2007
    ..An overview is given of the main features of the transporters present in the tonoplast of plant cells in terms of their function, regulation, and relationships with the microheterogeneity of the vacuome...
  101. ncbi Functional characterization of the twin-arginine translocation system in Campylobacter jejuni
    Gireesh Rajashekara
    Food Animal Health Research Program, Ohio Agricultural Research Development Center, Department of Veterinary Preventive Medicine, The Ohio State University, Wooster, Ohio 44691, USA
    Foodborne Pathog Dis 6:935-45. 2009
    ..jejuni to sustain colonization in chickens. These findings suggest that the TAT pathway affects Campylobacter physiology and contributes to stress responses, allowing this fastidious pathogen to adapt to various environmental conditions...

Research Grants62

  1. PATHOGENESIS OF CORNEAL EDEMA AFTER INTRAOCULAR SURGERY
    Henry Edelhauser; Fiscal Year: 2006
    ....
  2. 2010 and 2012 Membrane Transport Proteins
    Robert H Edwards; Fiscal Year: 2012
    ..An extremely diverse collection of membrane transport proteins together account for the flux of a wide range of molecules, including sugars, amino acids, metabolites, ..
  3. Extracellular pH modulation by Candida albicans in vitro and in vivo
    Michael C Lorenz; Fiscal Year: 2013
    ..Finally, we will use live cell imaging to dissect the intracellular trafficking of C. albicans in the macrophage and determine the role of alkalinization and ammonia release in modulating endocytic maturation. ..
  4. 2011 Multidrug Efflux Systems GRC
    Olga Lomovskaya; Fiscal Year: 2011
    ..PUBLIC HEALTH RELEVANCE: All living cells use special membrane transport proteins to keep their interiors free from toxic products of cellular metabolism and a variety of environmental ..
  5. CANDIDACIDAL MECHANISMS OF SALIVARY HISTATINS
    Mira Edgerton; Fiscal Year: 2013
    ..This approach will support our long-range goal to develop alternative peptide- based therapies for treatment of oral candidiasis, which is currently limited to a small group of antifungal drugs. ..
  6. STRUCTURE AND FUNCTION OF THE GLUCOSE TRANSPORTER
    Mike M Mueckler; Fiscal Year: 2010
    ..Glut family belongs to the Major Facilitator Superfamily (MFS), which comprises the largest group of membrane transport proteins. The Gluts are responsible for the exchange of glucose between the blood and the cytoplasm of cells, ..
  7. Structure and Mechanism of a Virtual Proton Pump
    Christopher Miller; Fiscal Year: 2013
    ..proposal is to determine the structure of AdiC, a member of a widespread and little-understood class of membrane transport proteins - the APC superfamily...
  8. Dynamics of the Lactose Permease of Escherichia coli
    HOWARD RONALD KABACK; Fiscal Year: 2013
    ..Like channels and ABC transporters, ion gradient-coupled membrane transport proteins are also highly relevant to human physiology and disease (e.g...
  9. Novel capture reagents for membrane protein structure determination
    Lan Guan; Fiscal Year: 2013
    ..The approach proposed here can serve as a general tool to enhance the probability of crystallization of membrane proteins and thereby facilitate their structure determination. ..
  10. MECHANISM OF CARBOHYDRATE TRANSPORT IN BACTERIA
    Milton Saier; Fiscal Year: 2000
    ..coli and other enteric bacteria that will be of use in the control of these pathogens and will be universally applicable to an understanding of regulatory phenomena in all living cells. ..
  11. Structural Basis for Mechanism of Secondary Transporters
    HOWARD KABACK; Fiscal Year: 2009
    ..LacY is a paradigm for a huge group of structurally related membrane transport proteins (the Major Facilitator Superfamily), as well as for membrane proteins in general...
  12. Characterization of iron transport in GBS
    KATHRYN CLANCY; Fiscal Year: 2004
    ..The potential of identifying novel therapeutic targets to prevent or treat infections is also proposed. ..
  13. Colon Barrier Breakdown in the Elderly
    James Mullin; Fiscal Year: 2005
    ..of paracellular diffusion across the lower GI tract because of the probes lacking affinity for any membrane transport proteins anywhere in the GI tract, and their transit time through the length of the GI tract...
  14. MECHANISM FOR CHARGED-RELATED NEPHROTOXICITY
    Darryl Peterson; Fiscal Year: 1993
    ..This raises the possibility that membrane transport proteins, including Na/H antiporters, may be taken up during the endocytic process and become trapped in a pool ..
  15. Biologic Ventricular Assist Device
    Robert Kloner; Fiscal Year: 2004
    ..Identification and characterization of transplants will be carried out with PCR analysis of the Y chromosome (male cells into female host), detailed histologic analysis and immunostaining for muscle cell markers. ..
  16. RECEPTOR MEDIATED UPTAKE OF IRON COMPLEXES IN BACTERIA
    MARK MCINTOSH; Fiscal Year: 1999
    ..Understanding the bacterial iron uptake process at a molecular level is therefore important to designing effective strategies to withhold this vital nutrient for potentially virulent organisms. ..
  17. STRUCTURES OF SIDEROPHORE MEMBRANE TRANSPORT PROTEINS
    Dick van der Helm; Fiscal Year: 2000
    ..This will be done with binding experiments and difference Fouriers. The intent of the proposal is to understand, in detail, the specific transport of large molecules across a membrane. ..
  18. Structure and Function of Neurotransmitter Transporters
    JAMES GOUAUX; Fiscal Year: 2009
    ..PUBLIC HEALTH RELEVANCE: Integral membrane transport proteins remove chemical messengers or neurotransmitters from special junctions between nerve cells called ..
  19. MEMBRANES AND ACTIVE TRANSPORT OF AMINO ACIDS
    GIOVANNA AMES; Fiscal Year: 2001
    ....
  20. Membrane Transport Proteins Gordon Research Conference
    Lydia Aguilar Bryan; Fiscal Year: 2009
    ..This application requests partial support for the 2008 and 2010 Gordon Research Conferences (GRC) on Membrane Transport Proteins. This is a highly successful GRC that focuses on recent discoveries in a diverse group of ATPases, ion ..
  21. Rapid in vitro substrate assay for the multi-drug resistance p-glycoprotein
    DONALD MELCHIOR; Fiscal Year: 2009
    ..The test thereby allows the pharmaceutical industry to evaluate, at an early stage, the suitability of drug candidates for continued development. The test is reliable, simple, rapid, inexpensive and amenable to robotics. ..
  22. Roles of ubiquitin protein ligases in endocytosis
    Linda Hicke; Fiscal Year: 2005
    ....
  23. PHYSIOLOGIC CHARACTERISTICS OF TREPONEMA PALLIDUM
    Steven Norris; Fiscal Year: 2004
    ..It is anticipated that the results of this study will improve understanding of the growth requirements, oxygen utilization, and regulatory systems of T. pallidum. ..
  24. EFFECTS OF ETHANOL ON HEPATIC ORGANIC ANION UPTAKE
    Barry Potter; Fiscal Year: 1992
    ..Using these, carrier mediated uptake will be measured and changes in the plasma membrane transport proteins quantitated...
  25. 2004 & 2006 Membrane Transport Proteins Gordon Conference
    JONATHAN JAVITCH; Fiscal Year: 2005
    Support is requested for a Gordon Research Conference on "Membrane Transport Proteins: Physiological and Pathophysiological Implications," to be held from October 3 to 8, 2004 at Les Diablerets, Switzerland, as well as the 2006 Membrane ..
  26. YEAST AMINO ACID PERMEASES
    JANETTA GARRETT; Fiscal Year: 2000
    ..Tryptophan uptake is of particular interest because there are many unusual phenotypes associated with low tryptophan levels in yeast. There are also interesting parallels between yeast tryptophan uptake and auxin response in plants. ..
  27. NUTRIENT TRANSPORT MECHANISMS IN MYCOBACTERIA
    Nancy Connell; Fiscal Year: 1999
    ..Mutant studies will contribute to new antimycobacterial drug design and vaccine development. ..
  28. MOLECULAR BIOLOGY OF MAMMALIAN ANION TRANSPORT
    Ron Kopito; Fiscal Year: 2000
    Anion exchangers (AE) constitute a diverse family of mammalian plasma membrane transport proteins which contribute to the regulation of intracellular and extracellular ionic homeostasis and in erythroid cells, to the stability of the ..
  29. RED CELL AQUAPORIN-1 WATER TRANSPORT PROTEIN
    Peter Agre; Fiscal Year: 2002
    ..Several membrane transport proteins have first been identified in red cells, and during previous years of this project, a 28kDa protein was ..
  30. CNS L PROLINE TRANSPORTER
    ROBERT FREMEAU; Fiscal Year: 1999
    ..high affinity L-proline transporter (PROT) is a member of a gene family of Na and Cl dependent plasma membrane transport proteins that includes transporters for several neurotransmitters, osmolytes, and metabolites...
  31. COLONIC PROTON PUMP--ROLE IN ELECTROLYTE ABSORPTION
    Jonathan Kaunitz; Fiscal Year: 1990
    ..trapped pH-sensitive dyes will be used to study modulation of intracellular pH by plasma membrane transport proteins in isolated colonic surface cells, with particular emphasis on evaluating the role of a proton-..
  32. Renal Oxalate Secretion
    Robert Freel; Fiscal Year: 2004
    ..While it has long been agreed that the kidney actively secretes oxalate and a number of membrane transport proteins have been identified that may transport the oxalate anion, it is remarkable that no vectorial transport ..
  33. Conference--Molecular & Cellular Bioenergetics
    Michael Forgac; Fiscal Year: 2003
    ..expanded to become a major platform for the discussion of mechanistic information obtained on integral membrane transport proteins using X-ray crystallography, spectroscopic techniques and molecular biological approaches...
  34. EXPRESSION & ROLE OF TA1 ONCOFETAL GENE--LIVER CANCER
    Nancy Thompson; Fiscal Year: 2003
    ..These studies will provide mechanistic insight into TA1 function and its role in liver carcinogenesis. ..
  35. MOLECULAR PROPERTIES OF A CGMP-ACTIVATED CHANNEL
    STANLEY GOLDIN; Fiscal Year: 1991
    ..Transport-specific fractionation uses reconstitution of membrane transport proteins into liposomes as a physical tool for transport protein purification in functionally active form...
  36. VOLATILE ANESTHETIC ACTIONS--MOLECULAR GENETIC ANALYSIS
    RALPH KEIL; Fiscal Year: 1999
    ..2) Characterization of effects of anesthetics on nutrient transport. Several lines of evidence from our laboratory indicate that amino acid transport plays a critical role in the volatile anesthetic response of yeast. ..
  37. Novel Genetic Approaches to Structure and Function of M*
    Howard Shuman; Fiscal Year: 2002
    ..to develop and evaluate the utility of new methods for identifying and isolating functional regions of membrane transport proteins. It is anticipated that these methods will aid in assigning specific functions to particular regions of ..
  38. Structural Biology of Membrane Transport Proteins
    Anna De Angelis; Fiscal Year: 2004
    ..The structures of the proteins that bind and transport heavy metals in bacteria will provide a molecular model to understand diseases related to improper heavy metal transport and to exposure to heavy metal toxins. ..
  39. PHARMACOGENETICS OF MEMBRANE TRANSPORTERS
    Kathleen Giacomini; Fiscal Year: 2009
    ..to continue and extend our multi-disciplinary research program focused on the pharmacogenetics of membrane transport proteins that play a role in drug response pathways...
  40. 2007 Mechanisms of Membrane Transport Gordon Conference
    PETER MALONEY; Fiscal Year: 2007
    ..met biannually for the past 40 years, largely focusing on areas related to the mechanistic aspects of membrane transport proteins. In 2007, we will continue to emphasize the striking advances and insights enabled by high-resolution ..
  41. 2009 Mechanisms of Membrane Transport
    Amy Davidson; Fiscal Year: 2009
    ..This conference has met biannually for over 40 years, focusing on areas mechanistic aspects of membrane transport proteins and channels...
  42. PROTEIN-PROTEIN INTERACTIONS IN MEMBRANE RECEPTORS
    GIOVANNA AMES; Fiscal Year: 1993
    It is proposed to study the nature of the interactions between membrane transport proteins and soluble proteins, making use of site-directed mutagenesis, biochemical techniques, and genetic suppression analysis...
  43. Gordon Research Conference: Membrane Transport Proteins
    Nancy Carrasco; Fiscal Year: 2002
    Support is requested for a Gordon Research Conference on "Membrane Transport Proteins: Physiological and Pathophysiological Implications," to be held from July 21 to 26, 2002, at Connecticut College in New London, CT...
  44. MEMBRANES AND ACTIVE TRANSPORT OF AMINO ACIDS
    GIOVANNA AMES; Fiscal Year: 2004
    ..This knowledge can then be applied to help develop therapeutic tools for human diseases. ..
  45. CHARACTERIZATION OF MEMBRANE TRANSPORT MECHANISMS
    Frederick Suchy; Fiscal Year: 1991
    ..Conference will address very recent technical developments and concepts in the characterization of membrane transport proteins. In considering the content of the meeting and possible participants, it was concluded that much of the ..
  46. STRUCTURES AND MECHANISMS OF MEMBRANE TRANSPORT PROTEINS
    Christopher Miller; Fiscal Year: 2000
    Support is requested for a conference "Structure and Mechanism of Membrane Transport Proteins" sponsored by the Society for General Physiologists, and held at the Marine Biological Laboratory, Woods Hole, September 7-9, 2000...
  47. MECHANISMS OF MEMBRANE TRANSPORT PROTEINS
    RAYMOND FRIZZELL; Fiscal Year: 1991
    This proposal seeks partial support for a Gordon Research Conference on Mechanisms of Membrane Transport Proteins, to be held August 12-16, 1991...
  48. MEMBRANE TRANSPORT PROTEINS
    H Kaback; Fiscal Year: 1993
    This application seeks partial support for a Gordon Research Conference on Membrane Transport Proteins to be held June 28 to July 2, 1993...
  49. ATP-DEPENDENT PROTEIN KINASES IN GRAM-POSITIVE BACTERIA
    Milton Saier; Fiscal Year: 2004
    ..abstract_text> ..
  50. Regulation of Urea Transport by Adrenal Steroids
    JEFF SANDS; Fiscal Year: 2005
    ..We also found that UT-A1 protein abundance is decreased during in vivo conditions associated with increased glucocorticoids. ..
  51. Functional Characterization of Leishmania Transporters
    Scott M Landfear; Fiscal Year: 2010
    ..Overall, this proposal will provide a detailed biochemical and genetic dissection of glucose transporter function and the essential role of these permeases in the parasite life cycle. ..
  52. Development of Assay for High Throughput Screen of Parasite Glucose Transporter
    Scott M Landfear; Fiscal Year: 2010
    ..Since glucose is an essential nutrient for these parasites, such compounds would kill the parasite and could act as novel drugs for treatment of the diseases they cause. ..
  53. Host-Microbial Control of Deoxycholate Producton
    Paul Dawson; Fiscal Year: 2005
    ..Aim 3: To cross the Slcl0a2 null allele into additional mouse strains as a first step toward cloning the genes responsible controlling deoxycholate production (colonization by the bai operon bacteria). ..
  54. Membrane Transport of NH3 and NH4+
    NAZIH NAKHOUL; Fiscal Year: 2008
    ..These are new properties of NH3/NH4+ transport that will help explain the role of NH3 and NH4+ in acidosis and their effect on transport of other ions a well. ..
  55. FUNCTIONS OF THE HEPATIC GAMMA-GLUTAMYL CYCLE
    Nazzareno Ballatori; Fiscal Year: 2007
    ....
  56. METHYLMERCURY TRANSPORT ACROSS CELL MEMBRANES
    Nazzareno Ballatori; Fiscal Year: 2005
    ..Evaluate the molecular mechanism by which NAC stimulates renal excretion of MeHg. IV. Test the hypothesis that urinary excretion of MeHg following an NAC oral challenge may be used as a new biomarker of MeHg exposure. ..
  57. MECHANISM OF INDUCTION OF OLFACTORY TUMORS BY ALACHLOR
    Mary Beth Genter; Fiscal Year: 2002
    ....
  58. Inner Membrane Protein Assembly in Bacteria
    Ross Dalbey; Fiscal Year: 2009
    ..These studies should impact the field of bacterial membrane protein biogenesis and advance the knowledge of analogous pathways in eukaryotic cells. ..