Genomes and Genes
iron sulfur proteins
Summary: A group of proteins possessing only the iron-sulfur complex as the prosthetic group. These proteins participate in all major pathways of electron transport: photosynthesis, respiration, hydroxylation and bacterial hydrogen and nitrogen fixation.
Publications276 found, 100 shown here
- Mouse models for Friedreich ataxia exhibit cardiomyopathy, sensory nerve defect and Fe-S enzyme deficiency followed by intramitochondrial iron depositsH Puccio
Institut de Genetique et de Biologie Moleculaire et Cellulaire, CNRS INSERM Universite Louis Pasteur, B P 163, Illkirch, CU de Strasbourg, France
Nat Genet 27:181-6. 2001..These mutant mice represent the first mammalian models to evaluate treatment strategies for the human disease...
- Germ-line mutations in nonsyndromic pheochromocytomaHartmut P H Neumann
Department of Nephrology and Hypertension, Albert Ludwigs University, Freiburg, Germany
N Engl J Med 346:1459-66. 2002....
- A suf operon requirement for Fe-S cluster assembly during iron starvation in Escherichia coliF Wayne Outten
Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, Bethesda, Maryland 20892, USA
Mol Microbiol 52:861-72. 2004..coli, whereas the suf operon is specifically adapted to synthesize Fe-S clusters when iron or sulphur metabolism is disrupted by iron starvation or oxidative stress...
- IscR, an Fe-S cluster-containing transcription factor, represses expression of Escherichia coli genes encoding Fe-S cluster assembly proteinsC J Schwartz
Department of Biomolecular Chemistry, Microbiology Doctoral Training Program, and Institute for Enzyme Research, University of Wisconsin, Madison, WI 53706, USA
Proc Natl Acad Sci U S A 98:14895-900. 2001....
- A comparative genomics approach to prediction of new members of regulonsK Tan
Department of Genetics, Washington University School of Medicine, St Louis, Missouri 63110 8232, USA
Genome Res 11:566-84. 2001..coli. By studying orthologous genes subject to regulation by the same transcription factor, we also gained understanding of the evolution of the entire regulatory systems...
- Biogenesis of iron-sulphur clusters in amitochondriate and apicomplexan protistsFrank Seeber
FB Biology Parasitology, Philipps Universitat Marburg, Karl von Frisch Strasse, D 35032, Marburg, Germany
Int J Parasitol 32:1207-17. 2002..In this article I will summarise the current view of [Fe-S] cluster biogenesis in eukaryotes and discuss its implications for amitochondriate protists and for the plastid-like organelle of apicomplexan parasites...
- Iron hydrogenases--ancient enzymes in modern eukaryotesDavid S Horner
Dipartimento di Fisiologia e Biochimica Generali, University of Milan, Via Celoria 26, 20133, Milan, Italy
Trends Biochem Sci 27:148-53. 2002..These unique enzymes have thus moved from the margins of eukaryotic biology to become the focus of intense speculation and interest. This article summarizes current knowledge of their distribution, evolution and biochemistry...
- Biphenyl dioxygenases: functional versatilities and directed evolutionKensuke Furukawa
Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, Hakozaki, Fukuoka 812 8581, Japan
J Bacteriol 186:5189-96. 2004
- Bacterial redox sensorsJeffrey Green
Krebs Institute for Biomolecular Research, Department of Molecular Biology and Biotechnology, University of Sheffield, Western Bank, Sheffield S10 2TN, United Kingdom
Nat Rev Microbiol 2:954-66. 2004..Advances in biochemical analyses are revealing a range of elegant solutions that have evolved to allow bacteria to sense different redox signals...
- Functional versatility in the CRP-FNR superfamily of transcription factors: FNR and FLPJ Green
Department of Molecular Biology and Biotechnology, University of Sheffield, Western Bank, Sheffield S10 2TN, UK
Adv Microb Physiol 44:1-34. 2001..And, at the level of RNA-polymerase-interaction, different combinations of three discrete regions contacting the polymerase (the activating regions) are used for polymerase recruitment and promoting transcription...
- Isolation of an oxygen-sensitive FNR protein of Escherichia coli: interaction at activator and repressor sites of FNR-controlled genesS B Melville
Department of Microbiology and Molecular Genetics, University of California, Los Angeles 90095, USA
Proc Natl Acad Sci U S A 93:1226-31. 1996..A model whereby the FNR protein exists in the cell as a monomer that assembles on the DNA under anaerobic conditions to form a dimer is discussed...
- Contrasting sensitivities of Escherichia coli aconitases A and B to oxidation and iron depletionShery Varghese
Department of Microbiology, University of Illinois, Urbana, IL 61801, USA
J Bacteriol 185:221-30. 2003..coli synthesizes a transport system. Thus, AcnB may be retained as the primary aconitase because the lability of its exposed cluster allows E. coli to sense and respond to iron depletion...
- Chloroplast monothiol glutaredoxins as scaffold proteins for the assembly and delivery of [2Fe-2S] clustersSibali Bandyopadhyay
Department of Chemistry, Centre for Metalloenzyme Studies, University of Georgia, Athens, GA, USA
EMBO J 27:1122-33. 2008..Alternatively, they may function in the storage and/or delivery of preformed Fe-S clusters or in the regulation of the chloroplastic Fe-S cluster assembly machinery...
- The yeast iron regulatory proteins Grx3/4 and Fra2 form heterodimeric complexes containing a [2Fe-2S] cluster with cysteinyl and histidyl ligationHaoran Li
Department of Chemistry and Biochemistry, University of South Carolina, Columbia, South Carolina 29208, USA
Biochemistry 48:9569-81. 2009..Overall, these results suggest that the ability of the Fra2-Grx3/4 complex to assemble a [2Fe-2S] cluster may act as a signal to control the iron regulon in response to cellular iron status in yeast...
- Hydrogenases: hydrogen-activating enzymesMichel Frey
Laboratoire de Cristallographie et de Cristallogenese des Proteines, Institut de Biologie Structurale Jean Pierre Ebel, CEA CNRS, 41 rue Jules Horowitz, 38027 Grenoble, France
Chembiochem 3:153-60. 2002
- Structure of an aromatic-ring-hydroxylating dioxygenase-naphthalene 1,2-dioxygenaseB Kauppi
Department of Molecular Biology, Swedish University of Agricultural Sciences, Uppsala, Sweden
Structure 6:571-86. 1998..The present study was conducted to provide essential structural information necessary for elucidating the mechanism of action of NDO...
- Structure, function, and formation of biological iron-sulfur clustersDeborah C Johnson
Department of Biochemistry, Virginia Polytechnic Institute, Blacksburg, Virginia 24061, USA
Annu Rev Biochem 74:247-81. 2005....
- Discovery of two novel radical S-adenosylmethionine proteins required for the assembly of an active [Fe] hydrogenaseMatthew C Posewitz
National Renewable Energy Laboratory, Colorado School of Mines, Golden, CO 80401, USA
J Biol Chem 279:25711-20. 2004..This represents the first report on the nature of the accessory genes required for the maturation of an active [Fe] hydrogenase...
- Mutations in the SDHB gene are associated with extra-adrenal and/or malignant phaeochromocytomasAnne Paule Gimenez-Roqueplo
AP HP, Hopital Europeen Georges Pompidou, Département de Génétique Moléculaire, Paris
Cancer Res 63:5615-21. 2003..These data strongly suggest that SDHB gene is a tumor suppressor gene and that the identification of germ-line mutations in SDHB gene in patients with ASPs should be considered as a high-risk factor for malignancy or recurrence...
- Deficiency of glutaredoxin 5 reveals Fe-S clusters are required for vertebrate haem synthesisRebecca A Wingert
Stem Cell Program and Division Hematology Oncology Children s Hospital and Dana Farber Cancer Institute, Howard Hughes Medical Institute, Harvard Medical School, Boston, Massachusetts 02115, USA
Nature 436:1035-39. 2005..These findings uncover a connection between haem biosynthesis and Fe-S clusters, indicating that haemoglobin production in the differentiating red cell is regulated through Fe-S cluster assembly...
- The inactivation of Fe-S cluster containing hydro-lyases by superoxideD H Flint
E I Du Pont de Nemours and Company, Central Research and Development, Experimental Station, Wilmington, Delaware 19880
J Biol Chem 268:22369-76. 1993..We report here that E. coli fumarase A, fumarase B, and aconitase are also inactivated in vivo by hyperbaric O2. Thermodynamic parameters for the oxidation of the cluster of aconitase by O2- and O2 are calculated...
- Hydrogen production. Green algae as a source of energyA Melis
Department of Plant and Microbial Biology, 111 Koshland Hall, University of California, Berkeley, CA 94720 3102, USA
Plant Physiol 127:740-8. 2001..Thus, processes that are presently conceptual in nature, or at a developmental stage in the laboratory, need to be encouraged, tested for feasibility, and otherwise applied toward commercialization...
- Components involved in assembly and dislocation of iron-sulfur clusters on the scaffold protein Isu1pUlrich Muhlenhoff
Institut für Zytobiologie und Zytopathologie, Philipps Universitat Marburg, Robert Koch Strasse 6, D 35033 Marburg, Germany
EMBO J 22:4815-25. 2003..We propose a model that dissects Fe/S cluster biogenesis into two major steps and assigns its central components to one of these two steps...
- Ssq1, a mitochondrial Hsp70 involved in iron-sulfur (Fe/S) center biogenesis. Similarities to and differences from its bacterial counterpartRafal Dutkiewicz
Department of Molecular and Cellular Biology, Faculty of Biotechnology, University of Gdansk, 24 Kladki, 80 822 Gdansk, Poland
J Biol Chem 278:29719-27. 2003..Thus, whereas the bacterial and mitochondrial chaperone systems share critical features, they possess significant biochemical differences as well...
- Reconstitution of ThiC in thiamine pyrimidine biosynthesis expands the radical SAM superfamilyAbhishek Chatterjee
Department of Chemistry and Chemical Biology, 120 Baker Laboratory, Cornell University, Ithaca, New York 14853 1301, USA
Nat Chem Biol 4:758-65. 2008..Structural comparisons reveal that HMP-P synthase is homologous to a group of adenosylcobalamin radical enzymes. This similarity supports an evolutionary relationship between these two superfamilies...
- The iron-containing domain is essential in Rad3 helicases for coupling of ATP hydrolysis to DNA translocation and for targeting the helicase to the single-stranded DNA-double-stranded DNA junctionRobert A Pugh
Department of Biochemistry, Department of Animal Sciences, University of Illinois at Urbana Champaign, Urbana, IL 61801, USA
J Biol Chem 283:1732-43. 2008..Although it interacts specifically with the junction, the enzyme binds tightly to ssDNA, and the single-stranded regions of the substrate are the major contributors to the energetics of FacRad3-substrate interactions...
- FNR and its role in oxygen-regulated gene expression in Escherichia coliS Spiro
Department of Molecular Biology and Biotechnology, University of Sheffield, U K
FEMS Microbiol Rev 6:399-428. 1990..The relationships between FNR and other oxygen-related systems in E. coli are discussed, as well as parallel systems in other organisms.(ABSTRACT TRUNCATED AT 400 WORDS)..
- The mitochondrial ATP-binding cassette transporter Abcb7 is essential in mice and participates in cytosolic iron-sulfur cluster biogenesisCorinne Pondarre
Department of Pathology, Children s Hospital and Harvard Medical School, Boston, MA 02115, USA
Hum Mol Genet 15:953-64. 2006..Altogether, these studies demonstrate the essential nature of Abcb7 in mammals and further substantiate a central role for mitochondria in the biogenesis of cytosolic Fe-S proteins...
- Regulation of the HscA ATPase reaction cycle by the co-chaperone HscB and the iron-sulfur cluster assembly protein IscUJonathan J Silberg
Department of Physiology and Biophysics, University of California, Irvine, California 92697, USA
J Biol Chem 279:53924-31. 2004..ATP T-state is regenerated. Because HscA nucleotide exchange is fast, the overall rate of the chaperone cycle in vivo will be determined by the availability of the IscU-HscB substrate-co-chaperone complex...
- Functional assignment of the ORF2-iscS-iscU-iscA-hscB-hscA-fdx-ORF3 gene cluster involved in the assembly of Fe-S clusters in Escherichia coliY Takahashi
Department of Biology, Graduate School of Science, Osaka University, Toyonaka, Osaka, 560 0043, Japan
J Biochem 126:917-26. 1999..We propose a model in which the fdx gene product functions as an intermediate site for Fe-S cluster assembly...
- Aconitase and mitochondrial iron-sulphur protein deficiency in Friedreich ataxiaA Rotig
INSERM U393, Département de génétique, Hopital Necker Enfants Malades, Paris, France
Nat Genet 17:215-7. 1997..Mutated frataxin triggers aconitase and mitochondrial Fe-S respiratory enzyme deficiency in FRDA, which should therefore be regarded as a mitochondrial disorder...
- The cysteine desulfurase, IscS, has a major role in in vivo Fe-S cluster formation in Escherichia coliC J Schwartz
Department of Biomolecular Chemistry, University of Wisconsin, Madison, WI 53706, USA
Proc Natl Acad Sci U S A 97:9009-14. 2000..The lack of IscS also had a general effect on the growth of E. coli because the iscS deletion strain grew at half the rate of wild type in many types of media even when the auxotrophies were satisfied...
- Contributions of the LPPVK motif of the iron-sulfur template protein IscU to interactions with the Hsc66-Hsc20 chaperone systemKevin G Hoff
Department of Physiology and Biophysics, University of California, Irvine, California 92697, USA
J Biol Chem 278:37582-9. 2003..The results are discussed in the context of a structural model based on the crystallographic structure of the DnaK peptide-binding domain...
- Iron-sulfur clusters: ever-expanding rolesMarc Fontecave
Laboratoire de Chimie et Biochimie des Centres Redox Biologiques, DRDC CB, CEA CNRS Université Joseph Fourier, CEA Grenoble, UMR 5047, 17 Avenue des Martyrs, 38504 Grenoble Cedex 09, France
Nat Chem Biol 2:171-4. 2006
- The prokaryotic complex iron-sulfur molybdoenzyme familyRichard A Rothery
Membrane Protein Research Group, Department of Biochemistry, 474 Medical Sciences Building, University of Alberta, Edmonton, Alberta, Canada T6G 2H7
Biochim Biophys Acta 1778:1897-929. 2008..We have used genome sequence data to establish that there is a bias against the presence of soluble periplasmic molybdoenzymes in bacteria lacking an outer membrane...
- Formation and properties of [4Fe-4S] clusters on the IscU scaffold proteinKala Chandramouli
Department of Chemistry and Center for Metalloenzyme Studies, University of Georgia, Athens, Georgia 30602, USA
Biochemistry 46:6804-11. 2007....
- MitoNEET is a uniquely folded 2Fe 2S outer mitochondrial membrane protein stabilized by pioglitazoneMark L Paddock
Department of Physics, University of California at San Diego, La Jolla, CA 92093, USA
Proc Natl Acad Sci U S A 104:14342-7. 2007..The biophysical properties of mitoNEET suggest that it may participate in a redox-sensitive signaling and/or in Fe-S cluster transfer...
- Mitochondrial Iba57p is required for Fe/S cluster formation on aconitase and activation of radical SAM enzymesCristy Gelling
Institut für Zytobiologie und Zytopathologie, Philipps Universitat Marburg, Robert Koch Str 6, 35032 Marburg, Germany
Mol Cell Biol 28:1851-61. 2008..In keeping with this idea, the human IBA57 homolog C1orf69 complements the iba57Delta growth defects, demonstrating its conserved function throughout the eukaryotic kingdom...
- HscA and HscB stimulate [2Fe-2S] cluster transfer from IscU to apoferredoxin in an ATP-dependent reactionKala Chandramouli
Department of Chemistry and Center for Metalloenzyme Studies, University of Georgia, Athens, Georgia 30602, USA
Biochemistry 45:11087-95. 2006..J., Tapley, T. L., Hoff, K. G., and Vickery, L. E. (2004) J. Biol. Chem. 279, 53924-53931], and mechanistic proposals for coupling of the HscA ATPase cycle with cluster transfer from [2Fe-2S]IscU to apo-IscFdx are discussed...
- Novel succinate dehydrogenase subunit B (SDHB) mutations in familial phaeochromocytomas and paragangliomas, but an absence of somatic SDHB mutations in sporadic phaeochromocytomasDiana E Benn
Cancer Genetics, Kolling Institute of Medical Research, Royal North Shore Hospital, Syney, NSW, Australia
Oncogene 22:1358-64. 2003..Therefore, we conclude that SDHB has a major role in the pathogenesis of familial phaeochromocytomas, but the possible role of SDHB in sporadic tumours showing allelic loss at 1p36 has yet to be ascertained...
- Crystal structure of a NifS-like protein from Thermotoga maritima: implications for iron sulphur cluster assemblyJ T Kaiser
Max Planck Institut fur Biochemie, Abteilung Strukturforschung, Am Klopferspitz 18a, Martinsried, D 82152, Germany
J Mol Biol 297:451-64. 2000....
- Mitoferrin is essential for erythroid iron assimilationGeorge C Shaw
Division of Hematology, Department of Medicine, Brigham and Women s Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA
Nature 440:96-100. 2006..Our data show that mfrn functions as the principal mitochondrial iron importer essential for haem biosynthesis in vertebrate erythroblasts...
- Iron-sulphur cluster biogenesis and mitochondrial iron homeostasisTracey A Rouault
National Institute of Child Health and Human Development, Cell Biology and Metabolism Branch, Bethesda, Maryland 20892, USA
Nat Rev Mol Cell Biol 6:345-51. 2005..Recent studies in model systems and humans reveal that iron-sulphur proteins have important roles in mitochondrial iron homeostasis and in the pathogenesis of the human disease Friedreich ataxia...
- Identification of FRA1 and FRA2 as genes involved in regulating the yeast iron regulon in response to decreased mitochondrial iron-sulfur cluster synthesisAttila Kumanovics
Department of Pathology and Departments of Biochemistry and Medicine, School of Medicine, University of Utah, Salt Lake City Utah 84132, USA
J Biol Chem 283:10276-86. 2008..These results show that the Fra-Grx complex is an intermediate between the production of mitochondrial Fe-S clusters and transcription of the iron regulon...
- NO sensing by FNR: regulation of the Escherichia coli NO-detoxifying flavohaemoglobin, HmpHugo Cruz-Ramos
The Krebs Institute for Biomolecular Research, Department of Molecular Biology and Biotechnology, The University of Sheffield, Sheffield S10 2TN, UK
EMBO J 21:3235-44. 2002..Thus, FNR can respond to not only O(2), but also NO, with major implications for global gene regulation in bacteria. We propose an NO-mediated mechanism of hmp regulation by which E.coli responds to NO challenge...
- Rieske business: structure-function of Rieske non-heme oxygenasesDaniel J Ferraro
Department of Biochemistry, University of Iowa Roy J and Lucille A Carver College of Medicine, 51 Newton Road, 4 403 BSB, Iowa City, IA 52242, USA
Biochem Biophys Res Commun 338:175-90. 2005..The orientation of binding of the substrate to the enzyme is hypothesized to control the substrate selectivity and regio-specificity of product formation...
- Mitochondrial remnant organelles of Giardia function in iron-sulphur protein maturationJorge Tovar
School of Biological Sciences, Royal Holloway, University of London, Egham, Surrey TW20 0EX, UK
Nature 426:172-6. 2003..Our results indicate that Giardia is not primitively amitochondrial and that it has retained a functional organelle derived from the original mitochondrial endosymbiont...
- Identification of the Mycobacterium tuberculosis SUF machinery as the exclusive mycobacterial system of [Fe-S] cluster assembly: evidence for its implication in the pathogen's survivalGaelle Huet
Department of Molecular Mechanisms of Mycobacterial Infections, Institut de Pharmacologie et Biologie Structurale UMR5089, C N R S Université Paul Sabatier Toulouse III, 205 route de Narbonne, F 31077 Toulouse cedex, France
J Bacteriol 187:6137-46. 2005..tuberculosis survival virtually through its implication in the bacterial resistance to iron limitation and oxidative stress. As such, Pps1 may represent an interesting molecular target for new antituberculous drugs...
- Evidence for lateral transfer of genes encoding ferredoxins, nitroreductases, NADH oxidase, and alcohol dehydrogenase 3 from anaerobic prokaryotes to Giardia lamblia and Entamoeba histolyticaJulie E J Nixon
Department of Immunology and Infectious Diseases, Harvard School of Public Health, Boston, Massachusetts 02115, USA
Eukaryot Cell 1:181-90. 2002..In further support of lateral transfer, the G. lamblia NADH oxidase and adh3 genes appeared to have an evolutionary history distinct from those of E. histolytica...
- The hydrogenase-like Nar1p is essential for maturation of cytosolic and nuclear iron-sulphur proteinsJanneke Balk
Institut für Zytobiologie und Zytopathologie, Philipps Universitat Marburg, Robert Koch Strasse, Marburg, Germany
EMBO J 23:2105-15. 2004..In conclusion, Nar1p represents a crucial, novel component of the emerging cytosolic Fe/S protein assembly machinery that catalyses an essential and ancient process in eukaryotes...
- A specialized mitochondrial molecular chaperone system: a role in formation of Fe/S centersE A Craig
Department of Biomolecular Chemistry, 1300 University Avenue, University of Wisconsin, Madison, Wisconsin 53706, USA
Cell Mol Life Sci 59:1658-65. 2002..However, evidence to date suggests an interaction with the scaffold protein on which a transient Fe/S center is assembled, and thus implies a role in either assembly of the center or its transfer to recipient proteins...
- Escherichia coli contains a protein that is homologous in function and N-terminal sequence to the protein encoded by the nifS gene of Azotobacter vinelandii and that can participate in the synthesis of the Fe-S cluster of dihydroxy-acid dehydrataseD H Flint
E I Du Pont de Nemours and Company, Central Research and Development, Experimental Station, Wilmington, Delaware 19880 0328, USA
J Biol Chem 271:16068-74. 1996..coli dihydroxy-acid dehydratase. A mechanism is proposed for the early stages of the synthesis of Fe-S clusters using this protein and sulfur in the S0 oxidation state...
- Direct evidence for mRNA binding and post-transcriptional regulation by Escherichia coli aconitasesY Tang
The Krebs Institute for Biomolecular Research, Department of Molecular Biology and Biotechnology, University of Sheffield, UK
Microbiology 145:3069-79. 1999..Thus by modulating translation via site-specific interactions between apo-enzyme and relevant transcripts, the aconitases provide a new and rapidly reacting component of the bacterial oxidative stress response...
- Multiple turnover transfer of [2Fe2S] clusters by the iron-sulfur cluster assembly scaffold proteins IscU and IscAFrancesco Bonomi
Section of Biochemistry, Dipartimento di Sciencze Molecolari Agroalimentari, University of Milan, Italy
J Biol Chem 280:29513-8. 2005....
- Identification of a twin-arginine leader-binding proteinI J Oresnik
Structural Biology Research Group, Department of Biological Sciences, University of Calgary, Calgary, Alberta, T2N 1N4, Canada
Mol Microbiol 40:323-31. 2001..A strain carrying a dmsD knock-out mutation showed a loss of anaerobic growth on glycerol-DMSO medium and reduced growth on glycerol-fumarate medium. This work suggests that DmsD is a twin-arginine leader-binding protein...
- Frataxin is essential for extramitochondrial Fe-S cluster proteins in mammalian tissuesAlain Martelli
Institut de Genetique et de Biologie Moleculaire et Cellulaire, 1 rue Laurent Fries BP 10142, Illkirch F 67400, France
Hum Mol Genet 16:2651-8. 2007..These results thus provide new cellular pathways that may contribute to molecular mechanisms of the disease...
- Inactivation of the Friedreich ataxia mouse gene leads to early embryonic lethality without iron accumulationM Cossee
Institut de Genetique et de Biologie Moleculaire et Cellulaire IGBMC, CNRS INSERM Universite Louis Pasteur, Hopitaux Universitaires de Strasbourg, 1 rue Laurent Fries BP 163, 67404 Illkirch, France
Hum Mol Genet 9:1219-26. 2000..Surprisingly, in the frataxin knockout mouse, no iron accumulation was observed during embryonic resorption, suggesting that cell death could be due to a mechanism independent of iron accumulation...
- Integration of three signals at the Escherichia coli nrf promoter: a role for Fis protein in catabolite repressionDouglas F Browning
School of Biosciences, The University of Birmingham, Birmingham B15 2TT, UK
Mol Microbiol 57:496-510. 2005..Hence, Fis protein is a major factor responsible for catabolite repression at the nrf promoter, and Fis can override activation by FNR and NarL or NarP...
- Acidic residues of yeast frataxin have an essential role in Fe-S cluster assemblyFrancoise Foury
Unite de Biochimie Physiologique, Universite Catholique de Louvain, Croix du Sud, 5 15, 1348 Louvain la Neuve, Belgium
EMBO Rep 8:194-9. 2007..Therefore, the acidic ridge is essential for the Yfh1 function and is likely to be involved in iron-mediated protein-protein interactions...
- Dre2, a conserved eukaryotic Fe/S cluster protein, functions in cytosolic Fe/S protein biogenesisYan Zhang
Department of Medicine, Division of Hematology Oncology, University of Pennsylvania, Philadelphia, PA 19104, USA
Mol Cell Biol 28:5569-82. 2008....
- Network of protein-protein interactions among iron-sulfur cluster assembly proteins in Escherichia coliUmechiyo Tokumoto
Department of Biology, Graduate School of Science, Osaka University, Machikaneyama, Toyonaka, Osaka 560 0043, Japan
J Biochem 131:713-9. 2002..Thus, the sequential association and dissociation among the IscS, IscU, IscA, HscB, HscA, Fdx, and ORF3 proteins may be a critical process in the assembly of Fe-S clusters...
- AtNAP7 is a plastidic SufC-like ATP-binding cassette/ATPase essential for Arabidopsis embryogenesisXiang Ming Xu
Department of Biology, University of Leicester, Leicester LE1 7RH, United Kingdom
Proc Natl Acad Sci U S A 101:9143-8. 2004....
- The DNA repair helicases XPD and FancJ have essential iron-sulfur domainsJana Rudolf
Centre for Biomolecular Sciences, University of St Andrews, North Haugh, St Andrews, Fife KY16 9ST, United Kingdom
Mol Cell 23:801-8. 2006..Clinically relevant mutations in patients with trichothiodystrophy (TTD) and Fanconi anemia disrupt the Fe-S clusters of XPD and FancJ and thereby abolish helicase activity...
- Crystal structure of the terminal oxygenase component of biphenyl dioxygenase derived from Rhodococcus sp. strain RHA1Yutaka Furusawa
Biological Information Research Center BIRC, National Institute of Advanced Industrial Science and Technology AIST, 2 41 6 Aomi, Koto ku, Tokyo 135 0064, Japan
J Mol Biol 342:1041-52. 2004..The cooperative actions of residues in the three groups seem to determine the substrate specificity of the enzyme...
- FNR-dependent activation of the class II dmsA and narG promoters of Escherichia coli requires FNR-activating regions 1 and 3K E Lamberg
Departments of Bacteriology and Biomolecular Chemistry, University of Wisconsin, Madison, WI 53706, USA
Mol Microbiol 38:817-27. 2000....
- Genome analysis of Chlamydomonas reinhardtii reveals the existence of multiple, compartmentalized iron-sulfur protein assembly machineries of different evolutionary originsJames Godman
Department of Plant Sciences, University of Cambridge, Cambridge CB2 3EA, United Kingdom
Genetics 179:59-68. 2008..As a haploid, unicellular organism with available forward and reverse genetic tools, Chlamydomonas provides an excellent model system to study Fe-S cluster assembly and its regulation in photosynthetic eukaryotes...
- Nucleotide sequence and gene-polypeptide relationships of the glpABC operon encoding the anaerobic sn-glycerol-3-phosphate dehydrogenase of Escherichia coli K-12S T Cole
Biochimie des Régulations Cellulaires, Institut Pasteur, Paris, France
J Bacteriol 170:2448-56. 1988..Analysis of the GlpC primary structure indicated that the protein lacked extended hydrophobic sequences with the potential to form alpha-helices but did contain several long segments capable of forming transmembrane amphipathic helices...
- Iron-sulfur cluster biosynthesisSibali Bandyopadhyay
Department of Chemistry and Center for Metalloenzyme Studies, University of Georgia, Athens, GA 30602, USA
Biochem Soc Trans 36:1112-9. 2008....
- Molecular genetic analysis of an FNR-dependent anaerobically inducible Escherichia coli promoterA I Bell
School of Biochemistry, University of Birmingham, UK
Mol Microbiol 4:1753-63. 1990..However expression from these hybrid promoters is not as efficiently repressed during aerobic growth, suggesting that the function of bound FNR is dependent on the sequence context of the FNR-binding sequence...
- Classification and phylogeny of hydrogenasesP M Vignais
CEA Grenoble, Départment de Biologie Moléculaire et Structurale, 17 Avenue des Martyrs, 38054 Grenoble Cedex 9, France
FEMS Microbiol Rev 25:455-501. 2001..On the basis of the comparisons performed and discussed here, proposals are made to simplify and rationalize the nomenclature of H2ase-encoding genes...
- Glutaredoxin 5 deficiency causes sideroblastic anemia by specifically impairing heme biosynthesis and depleting cytosolic iron in human erythroblastsHong Ye
Molecular Medicine Program, National Institute of Child Health and Human Development, NIH, Bethesda, Maryland 20892, USA
J Clin Invest 120:1749-61. 2010..Erythroblasts express both IRP-repressible ALAS2 and non-IRP-repressible ferroportin 1b. The unique combination of IRP targets likely accounts for the tissue-specific phenotype of human GLRX5 deficiency...
- A cluster exposed: structure of the Rieske ferredoxin from biphenyl dioxygenase and the redox properties of Rieske Fe-S proteinsC L Colbert
Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907, USA
Structure 8:1267-78. 2000..These distinctions were predicted to arise from differences in the exposure of the cluster and/or interactions of the histidine ligands...
- 2-Oxoquinoline 8-monooxygenase oxygenase component: active site modulation by Rieske-[2Fe-2S] center oxidation/reductionBerta Maria Martins
Laboratorium Proteinkristallographie, Universitat Bayreuth, Germany
Structure 13:817-24. 2005..This creates an additional coordination site at the mononuclear Fe(II) ion and can open a pathway for dioxygen to bind in the substrate-containing active site...
- Disabled early recruitment of antioxidant defenses in Friedreich's ataxiaK Chantrel-Groussard
, INSERM U393, Tour Lavoisier, , , 75743 Paris Cedex 15, France
Hum Mol Genet 10:2061-7. 2001..This suggests that continuous oxidative damage to iron-sulfur clusters, resulting from hampered superoxide dismutase signaling, is causative of the mitochondrial deficiency and long term mitochondrial iron overload occurring in FRDA...
- The chloroplastic protein import machinery contains a Rieske-type iron-sulfur cluster and a mononuclear iron-binding proteinA Caliebe
Botanisches Institut, , Am Botanischen Garten 1 9, D 24118 Kiel, Germany
EMBO J 16:7342-50. 1997..Together, these results strongly suggest that the 52 kDa protein, named Tic55 due to its calculated molecular weight, is a member of the chloroplastic inner envelope protein translocon...
- Metabolic maps and functions of the Plasmodium mitochondrionGiel G van Dooren
Plant Cell Biology Research Centre, School of Botany, University of Melbourne, Parkville, Victoria, Australia
FEMS Microbiol Rev 30:596-630. 2006..The function of the tricarboxylic acid cycle in the mitochondrion is unclear. We discuss directions for future research into this fascinating, yet enigmatic, organelle...
- Phylogeny of the bacterial superfamily of Crp-Fnr transcription regulators: exploiting the metabolic spectrum by controlling alternative gene programsHeinz Körner
Lehrstuhl fur Mikrobiologie, Universitat Karlsruhe, PF 6980, D 76128 Karlsruhe, Germany
FEMS Microbiol Rev 27:559-92. 2003..The regulatory adaptability and structural flexibility represented in the Crp-Fnr scaffold has led to the evolution of an important group of physiologically versatile transcription factors...
- Modulation of substrate binding to naphthalene 1,2-dioxygenase by rieske cluster reduction/oxidationTran Chin Yang
Department of Chemistry, Northwestern University, Evanston, Illinois 60208 3113, USA
J Am Chem Soc 125:2034-5. 2003..This shift, and the conformational coupling it implies, may hold the key to both oxygen gating and oxygen reactivity for Rieske aromatic dioxygenases...
- Substrate binding to NO-ferro-naphthalene 1,2-dioxygenase studied by high-resolution Q-band pulsed 2H-ENDOR spectroscopyTran Chin Yang
Department of Chemistry, Northwestern University, Evanston, Illinois 60208 3113, USA
J Am Chem Soc 125:7056-66. 2003..5 A farther from the Fe atom. The relative populations of A- and B-sites are allosterically changed when the Rieske cluster is reduced. ENDOR of exchangeable protons shows that the water/hydroxide of Fe-NDO is retained upon binding NO...
- The interface between the biological and inorganic worlds: iron-sulfur metalloclustersDouglas C Rees
Division of Chemistry and Chemical Engineering 114 96, Howard Hughes Medical Institute, California Institute of Technology, Pasadena, CA 91125, USA
Science 300:929-31. 2003....
- A novel eukaryotic factor for cytosolic Fe-S cluster assemblyAmit Roy
Department of Microbiology and Immunology, University of Illinois at Chicago, Chicago, IL 60612, USA
EMBO J 22:4826-35. 2003..Consistent with a cytosolic site of activity, Cfd1p was localized in the cytoplasm. To our knowledge, Cfd1p is the first cytoplasmic Fe-S cluster assembly factor described in eukaryotes...
- Mitochondrial dysfunction leads to nuclear genome instability via an iron-sulfur cluster defectJoshua R Veatch
Division of Basic Sciences, Fred Hutchinson Cancer Research Center, University of Washington, Seattle, WA 98109, USA
Cell 137:1247-58. 2009..For a video summary of this article, see the PaperFlick file available with the online Supplemental Data...
- Fe-S proteins in sensing and regulatory functionsH Beinert
Institute for Enzyme Research, Graduate School, Department of Biochemistry, College of Agricultural and Life Sciences, University of Wisconsin, 1710 University Avenue, Madison, WI 53705, USA
Curr Opin Chem Biol 3:152-7. 1999..We now know that the vulnerability of these structures to oxidative destruction is used by nature in sensing O2, iron, and possibly also nitric oxide. Changes in the oxidation state of Fe-S clusters can also serve as a reversible switch...
- The [Fe-Fe]-hydrogenase maturation protein HydF from Thermotoga maritima is a GTPase with an iron-sulfur clusterXavier Brazzolotto
Laboratoire de Chimie et Biochimie des Centres Redox Biologiques, UMR Université Joseph Fourier CNRS Commissariat à l Energie Atomique No 5047, Grenoble, France
J Biol Chem 281:769-74. 2006..HYSCORE spectroscopy experiments were carried out to determine the nature of the fourth ligand of the cluster, and its exchangeability was demonstrated with the formation of a [4Fe-4S]-imidazole complex...
- Functional analysis of Arabidopsis genes involved in mitochondrial iron-sulfur cluster assemblyAna Paula Guedes Frazzon
Department of Biological Sciences, Virginia Tech, Blacksburg, VA, USA
Plant Mol Biol 64:225-40. 2007....
- Pheochromocytoma--death of an axiomRobert G Dluhy
N Engl J Med 346:1486-8. 2002
- Biogenesis of iron-sulfur proteins in plantsJanneke Balk
Department of Plant Sciences, University of Cambridge, Downing Street, Cambridge, UK CB2 3EA
Trends Plant Sci 10:324-31. 2005..Recent findings now suggest that both plastids and mitochondria are capable of assembling Fe-S proteins using assembly machineries that differ in biochemical properties, genetic make-up and evolutionary origin...
- Pathways of oxidative damageJames A Imlay
Department of Microbiology, University of Illinois, Urbana, Illinois 61801, USA
Annu Rev Microbiol 57:395-418. 2003..The vulnerability of cells to oxidation lies at the root of obligate anaerobiosis, spontaneous mutagenesis, and the use of oxidative stress as a biological weapon...
- Genome-wide expression analysis indicates that FNR of Escherichia coli K-12 regulates a large number of genes of unknown functionYisheng Kang
Department of Genetics, 425 Henry Mall, University of Wisconsin, Madison, WI 53706, USA
J Bacteriol 187:1135-60. 2005..We suggest that E. coli MG1655 has a larger metabolic potential under anaerobic conditions than has been previously recognized...
- Splice mutation in the iron-sulfur cluster scaffold protein ISCU causes myopathy with exercise intoleranceFanny Mochel
Developmental and Metabolic Neurology Branch, NINDS, NIH, Bethesda, MD 20892, USA
Am J Hum Genet 82:652-60. 2008..ISCU interacts with the Friedreich ataxia gene product frataxin in iron-sulfur cluster biosynthesis. Our results therefore extend the range of known human diseases that are caused by defects in iron-sulfur cluster biogenesis...
- Identification of the Clostridium perfringens genes involved in the adaptive response to oxidative stressV Briolat
Unite de Genetique Moleculaire Bacterienne, Institut Pasteur, 75724 Paris Cedex 15, France
J Bacteriol 184:2333-43. 2002..g., a gene encoding an ATP-dependent RNA helicase, the beta-glucuronidase gene, and the gene encoding the atypical iron sulfur prismane protein) have been shown to be involved in the oxidative response...
- The eukaryotic P loop NTPase Nbp35: an essential component of the cytosolic and nuclear iron-sulfur protein assembly machineryAnja Hausmann
Institut für Zytobiologie und Zytopathologie, Philipps Universitat Marburg, Robert Koch Strasse 6, D 35033 Marburg, Germany
Proc Natl Acad Sci U S A 102:3266-71. 2005..The findings provide strong evidence for the existence of a highly conserved and essential machinery dedicated to assembling cytosolic and nuclear Fe/S proteins...
- ErpA, an iron sulfur (Fe S) protein of the A-type essential for respiratory metabolism in Escherichia coliLaurent Loiseau
Unité Propre de Recherche 9043, Laboratoire de Chimie Bacterienne, Centre National de la Recherche Scientifique, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
Proc Natl Acad Sci U S A 104:13626-31. 2007..coli. Surprisingly, the erpA gene maps at a distance from any other Fe-S biogenesis-related gene. ErpA is an A-type Fe-S protein that is characterized by an essential role in cellular metabolism...
- HydF as a scaffold protein in [FeFe] hydrogenase H-cluster biosynthesisShawn E McGlynn
Department of Chemistry and Biochemistry, The Astrobiology Biogeocatalysis Research Center, Montana State University, Bozeman, MT 59717, USA
FEBS Lett 582:2183-7. 2008..These results represent the first in vitro maturation of [FeFe] hydrogenase with purified proteins, and suggest that HydF functions as a scaffold upon which an H-cluster intermediate is synthesized...
- Aerobic degradation of polychlorinated biphenylsDietmar H Pieper
Department of Environmental Microbiology, German Research Center for Biotechnology, Mascheroder Weg 1, 38124, Braunschweig, Germany
Appl Microbiol Biotechnol 67:170-91. 2005..However, further research is necessary to understand the complex interactions between soil/sediment, pollutant, surfactant and microorganisms in different environments...
- The cymA gene, encoding a tetraheme c-type cytochrome, is required for arsenate respiration in Shewanella speciesJulie N Murphy
Department of Environmental Toxicology, University of California, Santa Cruz, Santa Cruz, CA 95064, USA
J Bacteriol 189:2283-90. 2007....
- Iron and oxidative stress in bacteriaD Touati
Departement de Microbiologie, Institut Jacques Monod, CNRS Universités Paris 6 et Paris 7, 2 place Jussieu, Paris Cedex 05, 75251, France
Arch Biochem Biophys 373:1-6. 2000....
- Functional consequences of a SDHB gene mutation in an apparently sporadic pheochromocytomaAnne Paule Gimenez-Roqueplo
Département de Génétique Moléculaire, Hopital Europeen Georges Pompidou, Assistance Publique Hopitaux de Paris, Paris, France
J Clin Endocrinol Metab 87:4771-4. 2002..This observation highlights the role of the complex II mitochondrial genes in the oxygen-sensing pathway and in the regulation of angiogenesis of neural crest-derived tumors...
- Essential role of Isd11 in mitochondrial iron-sulfur cluster synthesis on Isu scaffold proteinsNils Wiedemann
Institut fur Biochemie und Molekularbiologie, Universitat Freiburg, Freiburg, Germany
EMBO J 25:184-95. 2006..We conclude that Isd11 is an indispensable eukaryotic component of the mitochondrial machinery for biogenesis of Fe/S proteins...
- Structure and dynamics of the iron-sulfur cluster assembly scaffold protein IscU and its interaction with the cochaperone HscBJin Hae Kim
Biophysics Graduate Program, University of Wisconsin, Madison, Wisconsin 53706, USA
Biochemistry 48:6062-71. 2009..We conclude that the IscU-HscB complex exists as two (or more) distinct states that interconvert at a rate much faster than the rate of dissociation of the complex and that HscB binds to and stabilizes the ordered state of apo-IscU...
- A mutation of the mitochondrial ABC transporter Sta1 leads to dwarfism and chlorosis in the Arabidopsis mutant starikS Kushnir
Vakgroep Moleculaire Genetica and Departement Plantengenetica, Vlaams Interuniversitair Instituut voor Biotechnologie VIB, Universiteit Gent, K L Ledeganckstraat 35, B 9000 Gent, Belgium
Plant Cell 13:89-100. 2001..Taken together, our results suggest that plant mitochondria possess an evolutionarily conserved Fe/S cluster biosynthesis pathway, which is linked to the intracellular iron homeostasis by the function of Atm1p-like ABC transporters...
- ROLE OF B.FRAGILIS OXYGEN STRESS RESPONSE IN INFECTIONCharles J Smith; Fiscal Year: 2013..We will determine if the iron storage proteins can contribute to the repair of iron sulfur proteins and the in vivo expression of their cognate genes will be measured to gain insight into their role during ..
- ROLE OF B.FRAGILIS OXYGEN STRESS RESPONSE IN INFECTIONCharles Smith; Fiscal Year: 2009..We will determine if the iron storage proteins can contribute to the repair of iron sulfur proteins and the in vivo expression of their cognate genes will be measured to gain insight into their role during ..
- NMR SPECTROSCOPY OF IRON-SULFUR PROTEINSJohn L Markley; Fiscal Year: 2010..abstract_text> ..
- Assembly of Biological Iron-Sulfur ClustersMichael K Johnson; Fiscal Year: 2012..A molecular-level understanding of iron-sulfur cluster biogenesis is crucial for understanding a variety of human diseases involving anemias, myopathies and ataxias that arise from defects in iron-sulfur cluster assembly proteins. ..
- COMPUTER SIMULATIONS OF ELECTRON TRANSFER PROTEINSToshiko Ichiye; Fiscal Year: 2013..For instance, dysfunction in respiratory complex I has been associated with human neurodegenerative diseases and aging. ..
- MITOCHONDRIAL IRON-SULFUR PROTEINSMichael Johnson; Fiscal Year: 1991..In addition they address questions of fundamental importance in the area of biological nitrogen fixation...
- STRUCTURAL STUDIES OF ELECTRON TRANSPORT PROTEINSHazel Holden; Fiscal Year: 1992..In addition, what is learned from these bacterial systems will be directly applicable towards understanding the properties of electron-transfer proteins in higher organisms...
- Structure and Function of Iron-Sulfur ClustersMichael Weaver; Fiscal Year: 2009..Defects in iron-sulfur cluster containing molecules are known to be associated with mitochondrial diseases, and the ability to better model these systems may lend new insight into diseases such as Parkinson's. ..
- Tandem TIme-of-Flight Mass Spectromer for ProteomicsKERMIT MURRAY; Fiscal Year: 2009..The instrument will be operated by skilled technicians, overseen by broadly represented committee and available for use by the entire LSU scientific community. ..
- STRUCTURE, FUNCTION AND REACTIVITY--IRON-SULFUR PROTEINSBENJAMIN FEINBERG; Fiscal Year: 1992..6. Stereospecific resonance Raman, spectroscopic assignments will be made with specific CyS 13C and 2H derivatives. 7. The influence of specific substitutions on redox and electron transfer capabilities will be examined. ..
- Probing the Electronic Structure of Fe-S ClustersLai Sheng Wang; Fiscal Year: 2004..It is expected that the new experimental results from the proposed work will stimulate further theoretical advancements. ..
- ELECTRON EXCHANGE EFFECTS ON ELECTRON TRANSFER DYNAMICSJames McCusker; Fiscal Year: 2000....
- THEORETICAL STUDIES OF METALLOPROTEIN CHROMOPHORESRichard Friesner; Fiscal Year: 1992..Determination of the factors which control electron transport efficiency and ligand binding and the capacity to predict the effects of chemical modifications on these properties should greatly facilitate the drug design process...
- NEW ASPECTS OF STRUCTURE-FUNCTION--IRON-SULFUR PROTEINSHelmut Beinert; Fiscal Year: 1992....
- KINETIC STUDIES OF HEMERYTHRIN REACTIONSRALPH WILKINS; Fiscal Year: 1980..A comparison will be made of these reactions with hemerythrin from other worm species, e.g., Phascolopsis gouldii; mammalian respiratory proteins myoglobin and hemoglobin; and other polynuclear (iron-sulfur) proteins...
- RAMAN STUDIES OF SULFITE REDUCTASES AND OTHER ENZYMESKWOK YUE; Fiscal Year: 1991..We also propose to characterize the chromophores of other iron- sulfur proteins from sulfate reducing bacteria...
- Nitric Oxide Cytotoxicity and Iron-Sulfur ProteinsHuangen Ding; Fiscal Year: 2009..The proposed research, if successful, will provide fundamental knowledge for developing therapeutic approaches to prevent or alleviate cellular oxidative damages inflicted by NO cytotoxicity. ..
- The Physiology of Oxidative Stress in Escherichia coliJames Imlay; Fiscal Year: 2006..coli will provide a blueprint for efforts to solve key issues in oxidative stress: obligate anaerobiosis, the killing mechanism of) hagocytes, and endogenous oxidative stress in higher organisms. ..
- ELECTRONIC STRUCTURE OF IRON-SULFUR AND RELATED PROTEINSLouis Noodleman; Fiscal Year: 2010..Calculations and analysis of vibrational spectra and of hyperfine spectra compared with experiment are important indicators of stable intermediates over the catalytic cycle. All changes are in italics. ..
- Electronic Structure and Reactions of Fe-oxo EnzymesLouis Noodleman; Fiscal Year: 2010..A better understanding of these enzyme mechanisms should aid experimental work toward these goals. ..
- CRYSTALLOGRAPHIC STUDIES OF ELECTRON TRANSFER PROTEINSDouglas Rees; Fiscal Year: 2002..4. the correspondence between crystallographically defined metallocluster structures and spectroscopically assigned oxidation states in the Fe-protein and MoFe-protein. ..
- ENZYME MEDIATED THIOL MODIFICATION OF TRNACHARLES LAUHON; Fiscal Year: 2002....
- PROTON & ELECTRON TRANSFER & ENERGY COUPLING IN SITE ITOMOKO NONE OHNISHI; Fiscal Year: 2010..Using this new strategy, we found that both cluster N2 and flavin can generate superoxide. We will solidify this exciting finding and study its relevance to complex I function under physiological and pathological conditions. ..
- IRON METABOLISM AND PHOSPHORYLATION OF IRPS BY PKCRICHARD EISENSTEIN; Fiscal Year: 2002....
- MECHANISM OF OXIDATIVE DNA DAMAGE IN MODEL ORGANISMSJames Imlay; Fiscal Year: 2002..Further, by understanding how metabolic perturbations affect DNA oxidation, we may be able to anticipate the affects of drug therapies upon mutagenesis and cell death. ..
- GENETIC CONTROL OF NITRATE RESPIRATION IN E. COLIValley J Stewart; Fiscal Year: 2010..Thus, results from the model species E. coli enhance understanding for a broad range of pathogens that significantly impact public health. ..
- MODEL STUDIES OF ACETYL COENZYME A SYNTHASECharles Riordan; Fiscal Year: 2009..More broadly, a deeper understanding of ACS catalysis will advance fundamental understanding of redox-based Ni toxicity. ..
- Bioorganic Chemistry of Carbon Monoxide DehydrogenasePAUL LINDAHL; Fiscal Year: 2007..ACS/CODH is involved in C1 metabolism and it contains a tunnel through which CO migrates, impacting the field of metabolic channeling. ..
- Repair of Oxidatively Damaged GuaninesA Lien Lu Chang; Fiscal Year: 2008..We anticipate that these studies will advance our understanding of carcinogenesis process and form the background work for the development of new anti-cancer drugs. ..
- FUNCTIONAL STUDIES OF CYTOSOLIC ANTIOXIDANT PROTEINSJoan Selverstone Valentine; Fiscal Year: 2010..Together, these studies will shed light on the complex interactions of small molecules and proteins that all eukaryotes use to protect their mitochondria from superoxide-mediated damage. ..
- Genetic Analysis of Iron Homeostasis in C.ElegansELIZABETH ANN LEIBOLD; Fiscal Year: 2010..elegans using a green fluorescence protein (GFP)-based screen. ..
- Irradiation Damage and Protection of Pulmonary Endothelium Oxidative LipidomicsValerian E Kagan; Fiscal Year: 2010....
- Superoxide Generation from eNOS: The Role of PterinsJEANNETTE M VASQUEZ VIVAR; Fiscal Year: 2010..Generally this proposal is aimed at understanding these fundamental mechanisms that should provide the basis for developing new and improved strategies in the prevention and treatment of atherosclerosis and CVD. ..
- Structure of reaction intermediates in the bc1 complexSergei A Dikanov; Fiscal Year: 2010..The central role of bc1 complex plays out in many medical scenarios in which defects lead to pathology, among them cellular death through ROS-mediated damage, mitochondrial myopathies, and apoptosis, and in drug targeting. ..
- Connecting the Genome to the CytoplasmPamela A Silver; Fiscal Year: 2010..The experiments proposed in this grant will not only lead to new basic insights but also to new ways to analyze on a genome-wide level the situation in genetically defective cells and certain cancers. ..
- MECHANISM OF RESPIRATION AND ENERGY TRANSDUCTIONBernard Trumpower; Fiscal Year: 2009..abstract_text> ..
- Chaperone Function in Iron-Sulfur Protein FoldingLARRY VICKERY; Fiscal Year: 2008..abstract_text> ..
- X-ray studies of metalloenzyme regulation and catalysisCATHERINE DRENNAN; Fiscal Year: 2008..We will complement structural data with biochemical characterization and single crystal spectroscopic techniques. ..
- PORPHYRAZINE TUMOR CONTRAST AGENTSBrian Hoffman; Fiscal Year: 2004..GOAL: To correlate structural modifications of near-it absorbing/emitting pzs with their biological activity, so as to develop optimized, iv-deliverable optical contrast agents. ..
- HEME PROTEIN STRUCTURE AND FUNCTIONJack Peisach; Fiscal Year: 2004..abstract_text> ..
- IRON TRANSPORT IN MAMMALIAN CELLSPhilip Aisen; Fiscal Year: 2003..Project 5: Structure, function and dynamics in transferrin investigates the molecular mechanisms through which the uptake and release of iron and other metals by transferrin are modulated by physiological factors. ..
- COPPER/ALBUMIN REDOX-CYCLING IN PREECLAMPSIAValerian Kagan; Fiscal Year: 2003....
- Kinetic Simulations of Minimal Living SystemsPAUL LINDAHL; Fiscal Year: 2002..Simulation and animations will be critically compared to the properties of real cells. Mechanism will be modified, corrected, and embellished as deficiencies are noted and more experimental data become available. ..
- Gordon Research Conference on TetrapyrrolesGloria Ferreira; Fiscal Year: 2002..Strengths of this ongoing Gordon conference are its disciplinary diversity, focus on new unpublished work, time for discussion, informal atmosphere, and ban on dissemination of conference proceeding. ..
- Bruker Q-Band CW EPR SpectrometerBrian Hoffman; Fiscal Year: 2002..This must be replaced, and funds are requested for the purchase of a Bruker EMX 13/15 Q-band EP0R spectrometer with Q- band bridge and related equipment. This will be mated to the CW ENDOR apparatus in current use. ..
- REGULATION OF E COLI CYTOCHROME OXIDASE AND TCA CYCLERobert Gunsalus; Fiscal Year: 2001..coli. These studies should provide a detailed understanding of how the cell coordinates pathways for electron and carbon flow within the cell during transition between oxygen rich and oxygen limiting conditions. ..
- OXYGEN SENSING AND CELL SIGNALING IN HYPOXIAPaul Schumacker; Fiscal Year: 2001..These studies will identify a novel pathway of cellular O2 detection, and may help clarify understanding of how cells adapt to lowered O2 conditions. ..
- SUMMER RESEARCH IN MACROMOLECULAR INTERACTIONSCATHERINE DRENNAN; Fiscal Year: 2004..The proposed training project complements institutional goals of expanding career choices for students majoring in physical and quantitative sciences, and increasing minority participation in undergraduate research programs. ..
- THE HISTIDYL IMIDAZOLE LIGAND IN METALLOPROTEINSJOAN VALENTINE; Fiscal Year: 2005....
- MECHANISTIC STUDIES OF DNA MISMATCH REPAIRA Lien Lu Chang; Fiscal Year: 2006..Particularly, alterations in protein-protein interactions under oxidative stress will be investigated. These studies should advance our understanding of the role of DNA repair in genome stability and tumor susceptibility. ..
- ANAEROBIC EXPRESSION OF FUMARATE REDUCTASE IN E. COLIRobert Gunsalus; Fiscal Year: 2008..The Nar and Mode gene families will be identified by microarray analysis. Finally, the physiology of nitrate control will be examined by use of continuous culture methods. [unreadable] [unreadable]..
- Tetrahydrobiopterin in Hypoxia-Ischemia Induced Fetal Brain DysfunctionJeannette Vasquez Vivar; Fiscal Year: 2008..This information will likely define the use of tetrahydrobiopterin in the prevention and/or amelioration of H-I induced fetal brain dysfunction, which remains a major medical challenge. [unreadable] [unreadable] [unreadable]..
- Structure-Function of Hetero-oligomeric Integral Membrane ProteinsWILLIAM CRAMER; Fiscal Year: 2007..unreadable] [unreadable] [unreadable]..
- Role of Shigella two component regulation systems in intracellular adaptationLaura Runyen Janecky; Fiscal Year: 2007..The research described in this proposal will be useful for designing more effective therapies and will yield information applicable to other similar pathogens. [unreadable] [unreadable] [unreadable] [unreadable]..
- Receptor-mediated Colicin ImportWILLIAM CRAMER; Fiscal Year: 2007..2) The pathway of membrane insertion of the E1 channel domain, relative to the time course of other unfolding events, will be examined by time-resolved FRET using a large set of single Trp mutants. [unreadable] [unreadable]..
- Structures of Bacterial Membrane-Bound OxidoreductasesJoel Weiner; Fiscal Year: 2006....
- Directed evolution of P450 peroxide-mediated activityJONATHAN SILBERG; Fiscal Year: 2004....
- MECHANISMS OF ENZYMATIC REACTIONSPERRY FREY; Fiscal Year: 2006..unreadable] [unreadable]..
- ENZYME CATALYSIS OF ELECTRON AND GROUP TRANSFERPERRY FREY; Fiscal Year: 2006..The contributions of beta- amino acids produced by lysine 2,3-aminomutase and arginine 2,3-aminomutase to the functions of antibiotics are not known. The beta-aminoacids may become significant in drug development. ..
- IRON BINDING PROTEINS AND CONTROL OF IRON METABOLISMPhilip Aisen; Fiscal Year: 2001..Understanding the fundamental chemistry of iron transport, storage and utilization will provide the rational basis for managing the multitude of diseases where aberrations of iron metabolism contribute to cellular injury and death. ..