iron sulfur proteins

Summary

Summary: A group of proteins possessing only the iron-sulfur complex as the prosthetic group. These proteins participate in all major pathways of electron transport: photosynthesis, respiration, hydroxylation and bacterial hydrogen and nitrogen fixation.

Top Publications

  1. ncbi Mouse models for Friedreich ataxia exhibit cardiomyopathy, sensory nerve defect and Fe-S enzyme deficiency followed by intramitochondrial iron deposits
    H Puccio
    Institut de Genetique et de Biologie Moleculaire et Cellulaire, CNRS INSERM Universite Louis Pasteur, B P 163, Illkirch, CU de Strasbourg, France
    Nat Genet 27:181-6. 2001
  2. ncbi Germ-line mutations in nonsyndromic pheochromocytoma
    Hartmut P H Neumann
    Department of Nephrology and Hypertension, Albert Ludwigs University, Freiburg, Germany
    N Engl J Med 346:1459-66. 2002
  3. ncbi A suf operon requirement for Fe-S cluster assembly during iron starvation in Escherichia coli
    F Wayne Outten
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, Bethesda, Maryland 20892, USA
    Mol Microbiol 52:861-72. 2004
  4. pmc IscR, an Fe-S cluster-containing transcription factor, represses expression of Escherichia coli genes encoding Fe-S cluster assembly proteins
    C J Schwartz
    Department of Biomolecular Chemistry, Microbiology Doctoral Training Program, and Institute for Enzyme Research, University of Wisconsin, Madison, WI 53706, USA
    Proc Natl Acad Sci U S A 98:14895-900. 2001
  5. pmc A comparative genomics approach to prediction of new members of regulons
    K Tan
    Department of Genetics, Washington University School of Medicine, St Louis, Missouri 63110 8232, USA
    Genome Res 11:566-84. 2001
  6. ncbi Biogenesis of iron-sulphur clusters in amitochondriate and apicomplexan protists
    Frank Seeber
    FB Biology Parasitology, Philipps Universitat Marburg, Karl von Frisch Strasse, D 35032, Marburg, Germany
    Int J Parasitol 32:1207-17. 2002
  7. ncbi Iron hydrogenases--ancient enzymes in modern eukaryotes
    David S Horner
    Dipartimento di Fisiologia e Biochimica Generali, University of Milan, Via Celoria 26, 20133, Milan, Italy
    Trends Biochem Sci 27:148-53. 2002
  8. pmc Biphenyl dioxygenases: functional versatilities and directed evolution
    Kensuke Furukawa
    Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, Hakozaki, Fukuoka 812 8581, Japan
    J Bacteriol 186:5189-96. 2004
  9. ncbi Bacterial redox sensors
    Jeffrey Green
    Krebs Institute for Biomolecular Research, Department of Molecular Biology and Biotechnology, University of Sheffield, Western Bank, Sheffield S10 2TN, United Kingdom
    Nat Rev Microbiol 2:954-66. 2004
  10. ncbi Functional versatility in the CRP-FNR superfamily of transcription factors: FNR and FLP
    J Green
    Department of Molecular Biology and Biotechnology, University of Sheffield, Western Bank, Sheffield S10 2TN, UK
    Adv Microb Physiol 44:1-34. 2001

Research Grants

  1. ROLE OF B.FRAGILIS OXYGEN STRESS RESPONSE IN INFECTION
    Charles J Smith; Fiscal Year: 2013
  2. ROLE OF B.FRAGILIS OXYGEN STRESS RESPONSE IN INFECTION
    Charles Smith; Fiscal Year: 2009
  3. NMR SPECTROSCOPY OF IRON-SULFUR PROTEINS
    John L Markley; Fiscal Year: 2010
  4. Assembly of Biological Iron-Sulfur Clusters
    Michael K Johnson; Fiscal Year: 2012
  5. COMPUTER SIMULATIONS OF ELECTRON TRANSFER PROTEINS
    Toshiko Ichiye; Fiscal Year: 2013
  6. MITOCHONDRIAL IRON-SULFUR PROTEINS
    Michael Johnson; Fiscal Year: 1991
  7. STRUCTURAL STUDIES OF ELECTRON TRANSPORT PROTEINS
    Hazel Holden; Fiscal Year: 1992
  8. Structure and Function of Iron-Sulfur Clusters
    Michael Weaver; Fiscal Year: 2009
  9. Tandem TIme-of-Flight Mass Spectromer for Proteomics
    KERMIT MURRAY; Fiscal Year: 2009
  10. STRUCTURE, FUNCTION AND REACTIVITY--IRON-SULFUR PROTEINS
    BENJAMIN FEINBERG; Fiscal Year: 1992

Detail Information

Publications276 found, 100 shown here

  1. ncbi Mouse models for Friedreich ataxia exhibit cardiomyopathy, sensory nerve defect and Fe-S enzyme deficiency followed by intramitochondrial iron deposits
    H Puccio
    Institut de Genetique et de Biologie Moleculaire et Cellulaire, CNRS INSERM Universite Louis Pasteur, B P 163, Illkirch, CU de Strasbourg, France
    Nat Genet 27:181-6. 2001
    ..These mutant mice represent the first mammalian models to evaluate treatment strategies for the human disease...
  2. ncbi Germ-line mutations in nonsyndromic pheochromocytoma
    Hartmut P H Neumann
    Department of Nephrology and Hypertension, Albert Ludwigs University, Freiburg, Germany
    N Engl J Med 346:1459-66. 2002
    ....
  3. ncbi A suf operon requirement for Fe-S cluster assembly during iron starvation in Escherichia coli
    F Wayne Outten
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, Bethesda, Maryland 20892, USA
    Mol Microbiol 52:861-72. 2004
    ..coli, whereas the suf operon is specifically adapted to synthesize Fe-S clusters when iron or sulphur metabolism is disrupted by iron starvation or oxidative stress...
  4. pmc IscR, an Fe-S cluster-containing transcription factor, represses expression of Escherichia coli genes encoding Fe-S cluster assembly proteins
    C J Schwartz
    Department of Biomolecular Chemistry, Microbiology Doctoral Training Program, and Institute for Enzyme Research, University of Wisconsin, Madison, WI 53706, USA
    Proc Natl Acad Sci U S A 98:14895-900. 2001
    ....
  5. pmc A comparative genomics approach to prediction of new members of regulons
    K Tan
    Department of Genetics, Washington University School of Medicine, St Louis, Missouri 63110 8232, USA
    Genome Res 11:566-84. 2001
    ..coli. By studying orthologous genes subject to regulation by the same transcription factor, we also gained understanding of the evolution of the entire regulatory systems...
  6. ncbi Biogenesis of iron-sulphur clusters in amitochondriate and apicomplexan protists
    Frank Seeber
    FB Biology Parasitology, Philipps Universitat Marburg, Karl von Frisch Strasse, D 35032, Marburg, Germany
    Int J Parasitol 32:1207-17. 2002
    ..In this article I will summarise the current view of [Fe-S] cluster biogenesis in eukaryotes and discuss its implications for amitochondriate protists and for the plastid-like organelle of apicomplexan parasites...
  7. ncbi Iron hydrogenases--ancient enzymes in modern eukaryotes
    David S Horner
    Dipartimento di Fisiologia e Biochimica Generali, University of Milan, Via Celoria 26, 20133, Milan, Italy
    Trends Biochem Sci 27:148-53. 2002
    ..These unique enzymes have thus moved from the margins of eukaryotic biology to become the focus of intense speculation and interest. This article summarizes current knowledge of their distribution, evolution and biochemistry...
  8. pmc Biphenyl dioxygenases: functional versatilities and directed evolution
    Kensuke Furukawa
    Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, Hakozaki, Fukuoka 812 8581, Japan
    J Bacteriol 186:5189-96. 2004
  9. ncbi Bacterial redox sensors
    Jeffrey Green
    Krebs Institute for Biomolecular Research, Department of Molecular Biology and Biotechnology, University of Sheffield, Western Bank, Sheffield S10 2TN, United Kingdom
    Nat Rev Microbiol 2:954-66. 2004
    ..Advances in biochemical analyses are revealing a range of elegant solutions that have evolved to allow bacteria to sense different redox signals...
  10. ncbi Functional versatility in the CRP-FNR superfamily of transcription factors: FNR and FLP
    J Green
    Department of Molecular Biology and Biotechnology, University of Sheffield, Western Bank, Sheffield S10 2TN, UK
    Adv Microb Physiol 44:1-34. 2001
    ..And, at the level of RNA-polymerase-interaction, different combinations of three discrete regions contacting the polymerase (the activating regions) are used for polymerase recruitment and promoting transcription...
  11. pmc Isolation of an oxygen-sensitive FNR protein of Escherichia coli: interaction at activator and repressor sites of FNR-controlled genes
    S B Melville
    Department of Microbiology and Molecular Genetics, University of California, Los Angeles 90095, USA
    Proc Natl Acad Sci U S A 93:1226-31. 1996
    ..A model whereby the FNR protein exists in the cell as a monomer that assembles on the DNA under anaerobic conditions to form a dimer is discussed...
  12. pmc Contrasting sensitivities of Escherichia coli aconitases A and B to oxidation and iron depletion
    Shery Varghese
    Department of Microbiology, University of Illinois, Urbana, IL 61801, USA
    J Bacteriol 185:221-30. 2003
    ..coli synthesizes a transport system. Thus, AcnB may be retained as the primary aconitase because the lability of its exposed cluster allows E. coli to sense and respond to iron depletion...
  13. pmc Chloroplast monothiol glutaredoxins as scaffold proteins for the assembly and delivery of [2Fe-2S] clusters
    Sibali Bandyopadhyay
    Department of Chemistry, Centre for Metalloenzyme Studies, University of Georgia, Athens, GA, USA
    EMBO J 27:1122-33. 2008
    ..Alternatively, they may function in the storage and/or delivery of preformed Fe-S clusters or in the regulation of the chloroplastic Fe-S cluster assembly machinery...
  14. pmc The yeast iron regulatory proteins Grx3/4 and Fra2 form heterodimeric complexes containing a [2Fe-2S] cluster with cysteinyl and histidyl ligation
    Haoran Li
    Department of Chemistry and Biochemistry, University of South Carolina, Columbia, South Carolina 29208, USA
    Biochemistry 48:9569-81. 2009
    ..Overall, these results suggest that the ability of the Fra2-Grx3/4 complex to assemble a [2Fe-2S] cluster may act as a signal to control the iron regulon in response to cellular iron status in yeast...
  15. ncbi Hydrogenases: hydrogen-activating enzymes
    Michel Frey
    Laboratoire de Cristallographie et de Cristallogenese des Proteines, Institut de Biologie Structurale Jean Pierre Ebel, CEA CNRS, 41 rue Jules Horowitz, 38027 Grenoble, France
    Chembiochem 3:153-60. 2002
  16. ncbi Structure of an aromatic-ring-hydroxylating dioxygenase-naphthalene 1,2-dioxygenase
    B Kauppi
    Department of Molecular Biology, Swedish University of Agricultural Sciences, Uppsala, Sweden
    Structure 6:571-86. 1998
    ..The present study was conducted to provide essential structural information necessary for elucidating the mechanism of action of NDO...
  17. ncbi Structure, function, and formation of biological iron-sulfur clusters
    Deborah C Johnson
    Department of Biochemistry, Virginia Polytechnic Institute, Blacksburg, Virginia 24061, USA
    Annu Rev Biochem 74:247-81. 2005
    ....
  18. ncbi Discovery of two novel radical S-adenosylmethionine proteins required for the assembly of an active [Fe] hydrogenase
    Matthew C Posewitz
    National Renewable Energy Laboratory, Colorado School of Mines, Golden, CO 80401, USA
    J Biol Chem 279:25711-20. 2004
    ..This represents the first report on the nature of the accessory genes required for the maturation of an active [Fe] hydrogenase...
  19. ncbi Mutations in the SDHB gene are associated with extra-adrenal and/or malignant phaeochromocytomas
    Anne Paule Gimenez-Roqueplo
    AP HP, Hopital Europeen Georges Pompidou, Département de Génétique Moléculaire, Paris
    Cancer Res 63:5615-21. 2003
    ..These data strongly suggest that SDHB gene is a tumor suppressor gene and that the identification of germ-line mutations in SDHB gene in patients with ASPs should be considered as a high-risk factor for malignancy or recurrence...
  20. ncbi Deficiency of glutaredoxin 5 reveals Fe-S clusters are required for vertebrate haem synthesis
    Rebecca A Wingert
    Stem Cell Program and Division Hematology Oncology Children s Hospital and Dana Farber Cancer Institute, Howard Hughes Medical Institute, Harvard Medical School, Boston, Massachusetts 02115, USA
    Nature 436:1035-39. 2005
    ..These findings uncover a connection between haem biosynthesis and Fe-S clusters, indicating that haemoglobin production in the differentiating red cell is regulated through Fe-S cluster assembly...
  21. ncbi The inactivation of Fe-S cluster containing hydro-lyases by superoxide
    D H Flint
    E I Du Pont de Nemours and Company, Central Research and Development, Experimental Station, Wilmington, Delaware 19880
    J Biol Chem 268:22369-76. 1993
    ..We report here that E. coli fumarase A, fumarase B, and aconitase are also inactivated in vivo by hyperbaric O2. Thermodynamic parameters for the oxidation of the cluster of aconitase by O2- and O2 are calculated...
  22. pmc Hydrogen production. Green algae as a source of energy
    A Melis
    Department of Plant and Microbial Biology, 111 Koshland Hall, University of California, Berkeley, CA 94720 3102, USA
    Plant Physiol 127:740-8. 2001
    ..Thus, processes that are presently conceptual in nature, or at a developmental stage in the laboratory, need to be encouraged, tested for feasibility, and otherwise applied toward commercialization...
  23. pmc Components involved in assembly and dislocation of iron-sulfur clusters on the scaffold protein Isu1p
    Ulrich Muhlenhoff
    Institut für Zytobiologie und Zytopathologie, Philipps Universitat Marburg, Robert Koch Strasse 6, D 35033 Marburg, Germany
    EMBO J 22:4815-25. 2003
    ..We propose a model that dissects Fe/S cluster biogenesis into two major steps and assigns its central components to one of these two steps...
  24. ncbi Ssq1, a mitochondrial Hsp70 involved in iron-sulfur (Fe/S) center biogenesis. Similarities to and differences from its bacterial counterpart
    Rafal Dutkiewicz
    Department of Molecular and Cellular Biology, Faculty of Biotechnology, University of Gdansk, 24 Kladki, 80 822 Gdansk, Poland
    J Biol Chem 278:29719-27. 2003
    ..Thus, whereas the bacterial and mitochondrial chaperone systems share critical features, they possess significant biochemical differences as well...
  25. pmc Reconstitution of ThiC in thiamine pyrimidine biosynthesis expands the radical SAM superfamily
    Abhishek Chatterjee
    Department of Chemistry and Chemical Biology, 120 Baker Laboratory, Cornell University, Ithaca, New York 14853 1301, USA
    Nat Chem Biol 4:758-65. 2008
    ..Structural comparisons reveal that HMP-P synthase is homologous to a group of adenosylcobalamin radical enzymes. This similarity supports an evolutionary relationship between these two superfamilies...
  26. ncbi The iron-containing domain is essential in Rad3 helicases for coupling of ATP hydrolysis to DNA translocation and for targeting the helicase to the single-stranded DNA-double-stranded DNA junction
    Robert A Pugh
    Department of Biochemistry, Department of Animal Sciences, University of Illinois at Urbana Champaign, Urbana, IL 61801, USA
    J Biol Chem 283:1732-43. 2008
    ..Although it interacts specifically with the junction, the enzyme binds tightly to ssDNA, and the single-stranded regions of the substrate are the major contributors to the energetics of FacRad3-substrate interactions...
  27. ncbi FNR and its role in oxygen-regulated gene expression in Escherichia coli
    S Spiro
    Department of Molecular Biology and Biotechnology, University of Sheffield, U K
    FEMS Microbiol Rev 6:399-428. 1990
    ..The relationships between FNR and other oxygen-related systems in E. coli are discussed, as well as parallel systems in other organisms.(ABSTRACT TRUNCATED AT 400 WORDS)..
  28. ncbi The mitochondrial ATP-binding cassette transporter Abcb7 is essential in mice and participates in cytosolic iron-sulfur cluster biogenesis
    Corinne Pondarre
    Department of Pathology, Children s Hospital and Harvard Medical School, Boston, MA 02115, USA
    Hum Mol Genet 15:953-64. 2006
    ..Altogether, these studies demonstrate the essential nature of Abcb7 in mammals and further substantiate a central role for mitochondria in the biogenesis of cytosolic Fe-S proteins...
  29. ncbi Regulation of the HscA ATPase reaction cycle by the co-chaperone HscB and the iron-sulfur cluster assembly protein IscU
    Jonathan J Silberg
    Department of Physiology and Biophysics, University of California, Irvine, California 92697, USA
    J Biol Chem 279:53924-31. 2004
    ..ATP T-state is regenerated. Because HscA nucleotide exchange is fast, the overall rate of the chaperone cycle in vivo will be determined by the availability of the IscU-HscB substrate-co-chaperone complex...
  30. ncbi Functional assignment of the ORF2-iscS-iscU-iscA-hscB-hscA-fdx-ORF3 gene cluster involved in the assembly of Fe-S clusters in Escherichia coli
    Y Takahashi
    Department of Biology, Graduate School of Science, Osaka University, Toyonaka, Osaka, 560 0043, Japan
    J Biochem 126:917-26. 1999
    ..We propose a model in which the fdx gene product functions as an intermediate site for Fe-S cluster assembly...
  31. ncbi Aconitase and mitochondrial iron-sulphur protein deficiency in Friedreich ataxia
    A Rotig
    INSERM U393, Département de génétique, Hopital Necker Enfants Malades, Paris, France
    Nat Genet 17:215-7. 1997
    ..Mutated frataxin triggers aconitase and mitochondrial Fe-S respiratory enzyme deficiency in FRDA, which should therefore be regarded as a mitochondrial disorder...
  32. pmc The cysteine desulfurase, IscS, has a major role in in vivo Fe-S cluster formation in Escherichia coli
    C J Schwartz
    Department of Biomolecular Chemistry, University of Wisconsin, Madison, WI 53706, USA
    Proc Natl Acad Sci U S A 97:9009-14. 2000
    ..The lack of IscS also had a general effect on the growth of E. coli because the iscS deletion strain grew at half the rate of wild type in many types of media even when the auxotrophies were satisfied...
  33. ncbi Contributions of the LPPVK motif of the iron-sulfur template protein IscU to interactions with the Hsc66-Hsc20 chaperone system
    Kevin G Hoff
    Department of Physiology and Biophysics, University of California, Irvine, California 92697, USA
    J Biol Chem 278:37582-9. 2003
    ..The results are discussed in the context of a structural model based on the crystallographic structure of the DnaK peptide-binding domain...
  34. ncbi Iron-sulfur clusters: ever-expanding roles
    Marc Fontecave
    Laboratoire de Chimie et Biochimie des Centres Redox Biologiques, DRDC CB, CEA CNRS Université Joseph Fourier, CEA Grenoble, UMR 5047, 17 Avenue des Martyrs, 38504 Grenoble Cedex 09, France
    Nat Chem Biol 2:171-4. 2006
  35. ncbi The prokaryotic complex iron-sulfur molybdoenzyme family
    Richard A Rothery
    Membrane Protein Research Group, Department of Biochemistry, 474 Medical Sciences Building, University of Alberta, Edmonton, Alberta, Canada T6G 2H7
    Biochim Biophys Acta 1778:1897-929. 2008
    ..We have used genome sequence data to establish that there is a bias against the presence of soluble periplasmic molybdoenzymes in bacteria lacking an outer membrane...
  36. ncbi Formation and properties of [4Fe-4S] clusters on the IscU scaffold protein
    Kala Chandramouli
    Department of Chemistry and Center for Metalloenzyme Studies, University of Georgia, Athens, Georgia 30602, USA
    Biochemistry 46:6804-11. 2007
    ....
  37. pmc MitoNEET is a uniquely folded 2Fe 2S outer mitochondrial membrane protein stabilized by pioglitazone
    Mark L Paddock
    Department of Physics, University of California at San Diego, La Jolla, CA 92093, USA
    Proc Natl Acad Sci U S A 104:14342-7. 2007
    ..The biophysical properties of mitoNEET suggest that it may participate in a redox-sensitive signaling and/or in Fe-S cluster transfer...
  38. pmc Mitochondrial Iba57p is required for Fe/S cluster formation on aconitase and activation of radical SAM enzymes
    Cristy Gelling
    Institut für Zytobiologie und Zytopathologie, Philipps Universitat Marburg, Robert Koch Str 6, 35032 Marburg, Germany
    Mol Cell Biol 28:1851-61. 2008
    ..In keeping with this idea, the human IBA57 homolog C1orf69 complements the iba57Delta growth defects, demonstrating its conserved function throughout the eukaryotic kingdom...
  39. pmc HscA and HscB stimulate [2Fe-2S] cluster transfer from IscU to apoferredoxin in an ATP-dependent reaction
    Kala Chandramouli
    Department of Chemistry and Center for Metalloenzyme Studies, University of Georgia, Athens, Georgia 30602, USA
    Biochemistry 45:11087-95. 2006
    ..J., Tapley, T. L., Hoff, K. G., and Vickery, L. E. (2004) J. Biol. Chem. 279, 53924-53931], and mechanistic proposals for coupling of the HscA ATPase cycle with cluster transfer from [2Fe-2S]IscU to apo-IscFdx are discussed...
  40. ncbi Novel succinate dehydrogenase subunit B (SDHB) mutations in familial phaeochromocytomas and paragangliomas, but an absence of somatic SDHB mutations in sporadic phaeochromocytomas
    Diana E Benn
    Cancer Genetics, Kolling Institute of Medical Research, Royal North Shore Hospital, Syney, NSW, Australia
    Oncogene 22:1358-64. 2003
    ..Therefore, we conclude that SDHB has a major role in the pathogenesis of familial phaeochromocytomas, but the possible role of SDHB in sporadic tumours showing allelic loss at 1p36 has yet to be ascertained...
  41. ncbi Crystal structure of a NifS-like protein from Thermotoga maritima: implications for iron sulphur cluster assembly
    J T Kaiser
    Max Planck Institut fur Biochemie, Abteilung Strukturforschung, Am Klopferspitz 18a, Martinsried, D 82152, Germany
    J Mol Biol 297:451-64. 2000
    ....
  42. ncbi Mitoferrin is essential for erythroid iron assimilation
    George C Shaw
    Division of Hematology, Department of Medicine, Brigham and Women s Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA
    Nature 440:96-100. 2006
    ..Our data show that mfrn functions as the principal mitochondrial iron importer essential for haem biosynthesis in vertebrate erythroblasts...
  43. ncbi Iron-sulphur cluster biogenesis and mitochondrial iron homeostasis
    Tracey A Rouault
    National Institute of Child Health and Human Development, Cell Biology and Metabolism Branch, Bethesda, Maryland 20892, USA
    Nat Rev Mol Cell Biol 6:345-51. 2005
    ..Recent studies in model systems and humans reveal that iron-sulphur proteins have important roles in mitochondrial iron homeostasis and in the pathogenesis of the human disease Friedreich ataxia...
  44. pmc Identification of FRA1 and FRA2 as genes involved in regulating the yeast iron regulon in response to decreased mitochondrial iron-sulfur cluster synthesis
    Attila Kumanovics
    Department of Pathology and Departments of Biochemistry and Medicine, School of Medicine, University of Utah, Salt Lake City Utah 84132, USA
    J Biol Chem 283:10276-86. 2008
    ..These results show that the Fra-Grx complex is an intermediate between the production of mitochondrial Fe-S clusters and transcription of the iron regulon...
  45. pmc NO sensing by FNR: regulation of the Escherichia coli NO-detoxifying flavohaemoglobin, Hmp
    Hugo Cruz-Ramos
    The Krebs Institute for Biomolecular Research, Department of Molecular Biology and Biotechnology, The University of Sheffield, Sheffield S10 2TN, UK
    EMBO J 21:3235-44. 2002
    ..Thus, FNR can respond to not only O(2), but also NO, with major implications for global gene regulation in bacteria. We propose an NO-mediated mechanism of hmp regulation by which E.coli responds to NO challenge...
  46. ncbi Rieske business: structure-function of Rieske non-heme oxygenases
    Daniel J Ferraro
    Department of Biochemistry, University of Iowa Roy J and Lucille A Carver College of Medicine, 51 Newton Road, 4 403 BSB, Iowa City, IA 52242, USA
    Biochem Biophys Res Commun 338:175-90. 2005
    ..The orientation of binding of the substrate to the enzyme is hypothesized to control the substrate selectivity and regio-specificity of product formation...
  47. ncbi Mitochondrial remnant organelles of Giardia function in iron-sulphur protein maturation
    Jorge Tovar
    School of Biological Sciences, Royal Holloway, University of London, Egham, Surrey TW20 0EX, UK
    Nature 426:172-6. 2003
    ..Our results indicate that Giardia is not primitively amitochondrial and that it has retained a functional organelle derived from the original mitochondrial endosymbiont...
  48. pmc Identification of the Mycobacterium tuberculosis SUF machinery as the exclusive mycobacterial system of [Fe-S] cluster assembly: evidence for its implication in the pathogen's survival
    Gaelle Huet
    Department of Molecular Mechanisms of Mycobacterial Infections, Institut de Pharmacologie et Biologie Structurale UMR5089, C N R S Université Paul Sabatier Toulouse III, 205 route de Narbonne, F 31077 Toulouse cedex, France
    J Bacteriol 187:6137-46. 2005
    ..tuberculosis survival virtually through its implication in the bacterial resistance to iron limitation and oxidative stress. As such, Pps1 may represent an interesting molecular target for new antituberculous drugs...
  49. pmc Evidence for lateral transfer of genes encoding ferredoxins, nitroreductases, NADH oxidase, and alcohol dehydrogenase 3 from anaerobic prokaryotes to Giardia lamblia and Entamoeba histolytica
    Julie E J Nixon
    Department of Immunology and Infectious Diseases, Harvard School of Public Health, Boston, Massachusetts 02115, USA
    Eukaryot Cell 1:181-90. 2002
    ..In further support of lateral transfer, the G. lamblia NADH oxidase and adh3 genes appeared to have an evolutionary history distinct from those of E. histolytica...
  50. pmc The hydrogenase-like Nar1p is essential for maturation of cytosolic and nuclear iron-sulphur proteins
    Janneke Balk
    Institut für Zytobiologie und Zytopathologie, Philipps Universitat Marburg, Robert Koch Strasse, Marburg, Germany
    EMBO J 23:2105-15. 2004
    ..In conclusion, Nar1p represents a crucial, novel component of the emerging cytosolic Fe/S protein assembly machinery that catalyses an essential and ancient process in eukaryotes...
  51. ncbi A specialized mitochondrial molecular chaperone system: a role in formation of Fe/S centers
    E A Craig
    Department of Biomolecular Chemistry, 1300 University Avenue, University of Wisconsin, Madison, Wisconsin 53706, USA
    Cell Mol Life Sci 59:1658-65. 2002
    ..However, evidence to date suggests an interaction with the scaffold protein on which a transient Fe/S center is assembled, and thus implies a role in either assembly of the center or its transfer to recipient proteins...
  52. ncbi Escherichia coli contains a protein that is homologous in function and N-terminal sequence to the protein encoded by the nifS gene of Azotobacter vinelandii and that can participate in the synthesis of the Fe-S cluster of dihydroxy-acid dehydratase
    D H Flint
    E I Du Pont de Nemours and Company, Central Research and Development, Experimental Station, Wilmington, Delaware 19880 0328, USA
    J Biol Chem 271:16068-74. 1996
    ..coli dihydroxy-acid dehydratase. A mechanism is proposed for the early stages of the synthesis of Fe-S clusters using this protein and sulfur in the S0 oxidation state...
  53. ncbi Direct evidence for mRNA binding and post-transcriptional regulation by Escherichia coli aconitases
    Y Tang
    The Krebs Institute for Biomolecular Research, Department of Molecular Biology and Biotechnology, University of Sheffield, UK
    Microbiology 145:3069-79. 1999
    ..Thus by modulating translation via site-specific interactions between apo-enzyme and relevant transcripts, the aconitases provide a new and rapidly reacting component of the bacterial oxidative stress response...
  54. ncbi Multiple turnover transfer of [2Fe2S] clusters by the iron-sulfur cluster assembly scaffold proteins IscU and IscA
    Francesco Bonomi
    Section of Biochemistry, Dipartimento di Sciencze Molecolari Agroalimentari, University of Milan, Italy
    J Biol Chem 280:29513-8. 2005
    ....
  55. ncbi Identification of a twin-arginine leader-binding protein
    I J Oresnik
    Structural Biology Research Group, Department of Biological Sciences, University of Calgary, Calgary, Alberta, T2N 1N4, Canada
    Mol Microbiol 40:323-31. 2001
    ..A strain carrying a dmsD knock-out mutation showed a loss of anaerobic growth on glycerol-DMSO medium and reduced growth on glycerol-fumarate medium. This work suggests that DmsD is a twin-arginine leader-binding protein...
  56. ncbi Frataxin is essential for extramitochondrial Fe-S cluster proteins in mammalian tissues
    Alain Martelli
    Institut de Genetique et de Biologie Moleculaire et Cellulaire, 1 rue Laurent Fries BP 10142, Illkirch F 67400, France
    Hum Mol Genet 16:2651-8. 2007
    ..These results thus provide new cellular pathways that may contribute to molecular mechanisms of the disease...
  57. ncbi Inactivation of the Friedreich ataxia mouse gene leads to early embryonic lethality without iron accumulation
    M Cossee
    Institut de Genetique et de Biologie Moleculaire et Cellulaire IGBMC, CNRS INSERM Universite Louis Pasteur, Hopitaux Universitaires de Strasbourg, 1 rue Laurent Fries BP 163, 67404 Illkirch, France
    Hum Mol Genet 9:1219-26. 2000
    ..Surprisingly, in the frataxin knockout mouse, no iron accumulation was observed during embryonic resorption, suggesting that cell death could be due to a mechanism independent of iron accumulation...
  58. ncbi Integration of three signals at the Escherichia coli nrf promoter: a role for Fis protein in catabolite repression
    Douglas F Browning
    School of Biosciences, The University of Birmingham, Birmingham B15 2TT, UK
    Mol Microbiol 57:496-510. 2005
    ..Hence, Fis protein is a major factor responsible for catabolite repression at the nrf promoter, and Fis can override activation by FNR and NarL or NarP...
  59. pmc Acidic residues of yeast frataxin have an essential role in Fe-S cluster assembly
    Francoise Foury
    Unite de Biochimie Physiologique, Universite Catholique de Louvain, Croix du Sud, 5 15, 1348 Louvain la Neuve, Belgium
    EMBO Rep 8:194-9. 2007
    ..Therefore, the acidic ridge is essential for the Yfh1 function and is likely to be involved in iron-mediated protein-protein interactions...
  60. pmc Dre2, a conserved eukaryotic Fe/S cluster protein, functions in cytosolic Fe/S protein biogenesis
    Yan Zhang
    Department of Medicine, Division of Hematology Oncology, University of Pennsylvania, Philadelphia, PA 19104, USA
    Mol Cell Biol 28:5569-82. 2008
    ....
  61. ncbi Network of protein-protein interactions among iron-sulfur cluster assembly proteins in Escherichia coli
    Umechiyo Tokumoto
    Department of Biology, Graduate School of Science, Osaka University, Machikaneyama, Toyonaka, Osaka 560 0043, Japan
    J Biochem 131:713-9. 2002
    ..Thus, the sequential association and dissociation among the IscS, IscU, IscA, HscB, HscA, Fdx, and ORF3 proteins may be a critical process in the assembly of Fe-S clusters...
  62. pmc AtNAP7 is a plastidic SufC-like ATP-binding cassette/ATPase essential for Arabidopsis embryogenesis
    Xiang Ming Xu
    Department of Biology, University of Leicester, Leicester LE1 7RH, United Kingdom
    Proc Natl Acad Sci U S A 101:9143-8. 2004
    ....
  63. ncbi The DNA repair helicases XPD and FancJ have essential iron-sulfur domains
    Jana Rudolf
    Centre for Biomolecular Sciences, University of St Andrews, North Haugh, St Andrews, Fife KY16 9ST, United Kingdom
    Mol Cell 23:801-8. 2006
    ..Clinically relevant mutations in patients with trichothiodystrophy (TTD) and Fanconi anemia disrupt the Fe-S clusters of XPD and FancJ and thereby abolish helicase activity...
  64. ncbi Crystal structure of the terminal oxygenase component of biphenyl dioxygenase derived from Rhodococcus sp. strain RHA1
    Yutaka Furusawa
    Biological Information Research Center BIRC, National Institute of Advanced Industrial Science and Technology AIST, 2 41 6 Aomi, Koto ku, Tokyo 135 0064, Japan
    J Mol Biol 342:1041-52. 2004
    ..The cooperative actions of residues in the three groups seem to determine the substrate specificity of the enzyme...
  65. ncbi FNR-dependent activation of the class II dmsA and narG promoters of Escherichia coli requires FNR-activating regions 1 and 3
    K E Lamberg
    Departments of Bacteriology and Biomolecular Chemistry, University of Wisconsin, Madison, WI 53706, USA
    Mol Microbiol 38:817-27. 2000
    ....
  66. pmc Genome analysis of Chlamydomonas reinhardtii reveals the existence of multiple, compartmentalized iron-sulfur protein assembly machineries of different evolutionary origins
    James Godman
    Department of Plant Sciences, University of Cambridge, Cambridge CB2 3EA, United Kingdom
    Genetics 179:59-68. 2008
    ..As a haploid, unicellular organism with available forward and reverse genetic tools, Chlamydomonas provides an excellent model system to study Fe-S cluster assembly and its regulation in photosynthetic eukaryotes...
  67. pmc Nucleotide sequence and gene-polypeptide relationships of the glpABC operon encoding the anaerobic sn-glycerol-3-phosphate dehydrogenase of Escherichia coli K-12
    S T Cole
    Biochimie des Régulations Cellulaires, Institut Pasteur, Paris, France
    J Bacteriol 170:2448-56. 1988
    ..Analysis of the GlpC primary structure indicated that the protein lacked extended hydrophobic sequences with the potential to form alpha-helices but did contain several long segments capable of forming transmembrane amphipathic helices...
  68. pmc Iron-sulfur cluster biosynthesis
    Sibali Bandyopadhyay
    Department of Chemistry and Center for Metalloenzyme Studies, University of Georgia, Athens, GA 30602, USA
    Biochem Soc Trans 36:1112-9. 2008
    ....
  69. ncbi Molecular genetic analysis of an FNR-dependent anaerobically inducible Escherichia coli promoter
    A I Bell
    School of Biochemistry, University of Birmingham, UK
    Mol Microbiol 4:1753-63. 1990
    ..However expression from these hybrid promoters is not as efficiently repressed during aerobic growth, suggesting that the function of bound FNR is dependent on the sequence context of the FNR-binding sequence...
  70. ncbi Classification and phylogeny of hydrogenases
    P M Vignais
    CEA Grenoble, Départment de Biologie Moléculaire et Structurale, 17 Avenue des Martyrs, 38054 Grenoble Cedex 9, France
    FEMS Microbiol Rev 25:455-501. 2001
    ..On the basis of the comparisons performed and discussed here, proposals are made to simplify and rationalize the nomenclature of H2ase-encoding genes...
  71. pmc Glutaredoxin 5 deficiency causes sideroblastic anemia by specifically impairing heme biosynthesis and depleting cytosolic iron in human erythroblasts
    Hong Ye
    Molecular Medicine Program, National Institute of Child Health and Human Development, NIH, Bethesda, Maryland 20892, USA
    J Clin Invest 120:1749-61. 2010
    ..Erythroblasts express both IRP-repressible ALAS2 and non-IRP-repressible ferroportin 1b. The unique combination of IRP targets likely accounts for the tissue-specific phenotype of human GLRX5 deficiency...
  72. ncbi A cluster exposed: structure of the Rieske ferredoxin from biphenyl dioxygenase and the redox properties of Rieske Fe-S proteins
    C L Colbert
    Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907, USA
    Structure 8:1267-78. 2000
    ..These distinctions were predicted to arise from differences in the exposure of the cluster and/or interactions of the histidine ligands...
  73. ncbi 2-Oxoquinoline 8-monooxygenase oxygenase component: active site modulation by Rieske-[2Fe-2S] center oxidation/reduction
    Berta Maria Martins
    Laboratorium Proteinkristallographie, Universitat Bayreuth, Germany
    Structure 13:817-24. 2005
    ..This creates an additional coordination site at the mononuclear Fe(II) ion and can open a pathway for dioxygen to bind in the substrate-containing active site...
  74. ncbi Disabled early recruitment of antioxidant defenses in Friedreich's ataxia
    K Chantrel-Groussard
    , INSERM U393, Tour Lavoisier, , , 75743 Paris Cedex 15, France
    Hum Mol Genet 10:2061-7. 2001
    ..This suggests that continuous oxidative damage to iron-sulfur clusters, resulting from hampered superoxide dismutase signaling, is causative of the mitochondrial deficiency and long term mitochondrial iron overload occurring in FRDA...
  75. pmc The chloroplastic protein import machinery contains a Rieske-type iron-sulfur cluster and a mononuclear iron-binding protein
    A Caliebe
    Botanisches Institut, , Am Botanischen Garten 1 9, D 24118 Kiel, Germany
    EMBO J 16:7342-50. 1997
    ..Together, these results strongly suggest that the 52 kDa protein, named Tic55 due to its calculated molecular weight, is a member of the chloroplastic inner envelope protein translocon...
  76. ncbi Metabolic maps and functions of the Plasmodium mitochondrion
    Giel G van Dooren
    Plant Cell Biology Research Centre, School of Botany, University of Melbourne, Parkville, Victoria, Australia
    FEMS Microbiol Rev 30:596-630. 2006
    ..The function of the tricarboxylic acid cycle in the mitochondrion is unclear. We discuss directions for future research into this fascinating, yet enigmatic, organelle...
  77. ncbi Phylogeny of the bacterial superfamily of Crp-Fnr transcription regulators: exploiting the metabolic spectrum by controlling alternative gene programs
    Heinz Körner
    Lehrstuhl fur Mikrobiologie, Universitat Karlsruhe, PF 6980, D 76128 Karlsruhe, Germany
    FEMS Microbiol Rev 27:559-92. 2003
    ..The regulatory adaptability and structural flexibility represented in the Crp-Fnr scaffold has led to the evolution of an important group of physiologically versatile transcription factors...
  78. ncbi Modulation of substrate binding to naphthalene 1,2-dioxygenase by rieske cluster reduction/oxidation
    Tran Chin Yang
    Department of Chemistry, Northwestern University, Evanston, Illinois 60208 3113, USA
    J Am Chem Soc 125:2034-5. 2003
    ..This shift, and the conformational coupling it implies, may hold the key to both oxygen gating and oxygen reactivity for Rieske aromatic dioxygenases...
  79. ncbi Substrate binding to NO-ferro-naphthalene 1,2-dioxygenase studied by high-resolution Q-band pulsed 2H-ENDOR spectroscopy
    Tran Chin Yang
    Department of Chemistry, Northwestern University, Evanston, Illinois 60208 3113, USA
    J Am Chem Soc 125:7056-66. 2003
    ..5 A farther from the Fe atom. The relative populations of A- and B-sites are allosterically changed when the Rieske cluster is reduced. ENDOR of exchangeable protons shows that the water/hydroxide of Fe-NDO is retained upon binding NO...
  80. ncbi The interface between the biological and inorganic worlds: iron-sulfur metalloclusters
    Douglas C Rees
    Division of Chemistry and Chemical Engineering 114 96, Howard Hughes Medical Institute, California Institute of Technology, Pasadena, CA 91125, USA
    Science 300:929-31. 2003
    ....
  81. pmc A novel eukaryotic factor for cytosolic Fe-S cluster assembly
    Amit Roy
    Department of Microbiology and Immunology, University of Illinois at Chicago, Chicago, IL 60612, USA
    EMBO J 22:4826-35. 2003
    ..Consistent with a cytosolic site of activity, Cfd1p was localized in the cytoplasm. To our knowledge, Cfd1p is the first cytoplasmic Fe-S cluster assembly factor described in eukaryotes...
  82. pmc Mitochondrial dysfunction leads to nuclear genome instability via an iron-sulfur cluster defect
    Joshua R Veatch
    Division of Basic Sciences, Fred Hutchinson Cancer Research Center, University of Washington, Seattle, WA 98109, USA
    Cell 137:1247-58. 2009
    ..For a video summary of this article, see the PaperFlick file available with the online Supplemental Data...
  83. ncbi Fe-S proteins in sensing and regulatory functions
    H Beinert
    Institute for Enzyme Research, Graduate School, Department of Biochemistry, College of Agricultural and Life Sciences, University of Wisconsin, 1710 University Avenue, Madison, WI 53705, USA
    Curr Opin Chem Biol 3:152-7. 1999
    ..We now know that the vulnerability of these structures to oxidative destruction is used by nature in sensing O2, iron, and possibly also nitric oxide. Changes in the oxidation state of Fe-S clusters can also serve as a reversible switch...
  84. ncbi The [Fe-Fe]-hydrogenase maturation protein HydF from Thermotoga maritima is a GTPase with an iron-sulfur cluster
    Xavier Brazzolotto
    Laboratoire de Chimie et Biochimie des Centres Redox Biologiques, UMR Université Joseph Fourier CNRS Commissariat à l Energie Atomique No 5047, Grenoble, France
    J Biol Chem 281:769-74. 2006
    ..HYSCORE spectroscopy experiments were carried out to determine the nature of the fourth ligand of the cluster, and its exchangeability was demonstrated with the formation of a [4Fe-4S]-imidazole complex...
  85. ncbi Functional analysis of Arabidopsis genes involved in mitochondrial iron-sulfur cluster assembly
    Ana Paula Guedes Frazzon
    Department of Biological Sciences, Virginia Tech, Blacksburg, VA, USA
    Plant Mol Biol 64:225-40. 2007
    ....
  86. ncbi Pheochromocytoma--death of an axiom
    Robert G Dluhy
    N Engl J Med 346:1486-8. 2002
  87. ncbi Biogenesis of iron-sulfur proteins in plants
    Janneke Balk
    Department of Plant Sciences, University of Cambridge, Downing Street, Cambridge, UK CB2 3EA
    Trends Plant Sci 10:324-31. 2005
    ..Recent findings now suggest that both plastids and mitochondria are capable of assembling Fe-S proteins using assembly machineries that differ in biochemical properties, genetic make-up and evolutionary origin...
  88. ncbi Pathways of oxidative damage
    James A Imlay
    Department of Microbiology, University of Illinois, Urbana, Illinois 61801, USA
    Annu Rev Microbiol 57:395-418. 2003
    ..The vulnerability of cells to oxidation lies at the root of obligate anaerobiosis, spontaneous mutagenesis, and the use of oxidative stress as a biological weapon...
  89. pmc Genome-wide expression analysis indicates that FNR of Escherichia coli K-12 regulates a large number of genes of unknown function
    Yisheng Kang
    Department of Genetics, 425 Henry Mall, University of Wisconsin, Madison, WI 53706, USA
    J Bacteriol 187:1135-60. 2005
    ..We suggest that E. coli MG1655 has a larger metabolic potential under anaerobic conditions than has been previously recognized...
  90. pmc Splice mutation in the iron-sulfur cluster scaffold protein ISCU causes myopathy with exercise intolerance
    Fanny Mochel
    Developmental and Metabolic Neurology Branch, NINDS, NIH, Bethesda, MD 20892, USA
    Am J Hum Genet 82:652-60. 2008
    ..ISCU interacts with the Friedreich ataxia gene product frataxin in iron-sulfur cluster biosynthesis. Our results therefore extend the range of known human diseases that are caused by defects in iron-sulfur cluster biogenesis...
  91. pmc Identification of the Clostridium perfringens genes involved in the adaptive response to oxidative stress
    V Briolat
    Unite de Genetique Moleculaire Bacterienne, Institut Pasteur, 75724 Paris Cedex 15, France
    J Bacteriol 184:2333-43. 2002
    ..g., a gene encoding an ATP-dependent RNA helicase, the beta-glucuronidase gene, and the gene encoding the atypical iron sulfur prismane protein) have been shown to be involved in the oxidative response...
  92. pmc The eukaryotic P loop NTPase Nbp35: an essential component of the cytosolic and nuclear iron-sulfur protein assembly machinery
    Anja Hausmann
    Institut für Zytobiologie und Zytopathologie, Philipps Universitat Marburg, Robert Koch Strasse 6, D 35033 Marburg, Germany
    Proc Natl Acad Sci U S A 102:3266-71. 2005
    ..The findings provide strong evidence for the existence of a highly conserved and essential machinery dedicated to assembling cytosolic and nuclear Fe/S proteins...
  93. pmc ErpA, an iron sulfur (Fe S) protein of the A-type essential for respiratory metabolism in Escherichia coli
    Laurent Loiseau
    Unité Propre de Recherche 9043, Laboratoire de Chimie Bacterienne, Centre National de la Recherche Scientifique, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
    Proc Natl Acad Sci U S A 104:13626-31. 2007
    ..coli. Surprisingly, the erpA gene maps at a distance from any other Fe-S biogenesis-related gene. ErpA is an A-type Fe-S protein that is characterized by an essential role in cellular metabolism...
  94. ncbi HydF as a scaffold protein in [FeFe] hydrogenase H-cluster biosynthesis
    Shawn E McGlynn
    Department of Chemistry and Biochemistry, The Astrobiology Biogeocatalysis Research Center, Montana State University, Bozeman, MT 59717, USA
    FEBS Lett 582:2183-7. 2008
    ..These results represent the first in vitro maturation of [FeFe] hydrogenase with purified proteins, and suggest that HydF functions as a scaffold upon which an H-cluster intermediate is synthesized...
  95. ncbi Aerobic degradation of polychlorinated biphenyls
    Dietmar H Pieper
    Department of Environmental Microbiology, German Research Center for Biotechnology, Mascheroder Weg 1, 38124, Braunschweig, Germany
    Appl Microbiol Biotechnol 67:170-91. 2005
    ..However, further research is necessary to understand the complex interactions between soil/sediment, pollutant, surfactant and microorganisms in different environments...
  96. pmc The cymA gene, encoding a tetraheme c-type cytochrome, is required for arsenate respiration in Shewanella species
    Julie N Murphy
    Department of Environmental Toxicology, University of California, Santa Cruz, Santa Cruz, CA 95064, USA
    J Bacteriol 189:2283-90. 2007
    ....
  97. ncbi Iron and oxidative stress in bacteria
    D Touati
    Departement de Microbiologie, Institut Jacques Monod, CNRS Universités Paris 6 et Paris 7, 2 place Jussieu, Paris Cedex 05, 75251, France
    Arch Biochem Biophys 373:1-6. 2000
    ....
  98. ncbi Functional consequences of a SDHB gene mutation in an apparently sporadic pheochromocytoma
    Anne Paule Gimenez-Roqueplo
    Département de Génétique Moléculaire, Hopital Europeen Georges Pompidou, Assistance Publique Hopitaux de Paris, Paris, France
    J Clin Endocrinol Metab 87:4771-4. 2002
    ..This observation highlights the role of the complex II mitochondrial genes in the oxygen-sensing pathway and in the regulation of angiogenesis of neural crest-derived tumors...
  99. pmc Essential role of Isd11 in mitochondrial iron-sulfur cluster synthesis on Isu scaffold proteins
    Nils Wiedemann
    Institut fur Biochemie und Molekularbiologie, Universitat Freiburg, Freiburg, Germany
    EMBO J 25:184-95. 2006
    ..We conclude that Isd11 is an indispensable eukaryotic component of the mitochondrial machinery for biogenesis of Fe/S proteins...
  100. pmc Structure and dynamics of the iron-sulfur cluster assembly scaffold protein IscU and its interaction with the cochaperone HscB
    Jin Hae Kim
    Biophysics Graduate Program, University of Wisconsin, Madison, Wisconsin 53706, USA
    Biochemistry 48:6062-71. 2009
    ..We conclude that the IscU-HscB complex exists as two (or more) distinct states that interconvert at a rate much faster than the rate of dissociation of the complex and that HscB binds to and stabilizes the ordered state of apo-IscU...
  101. pmc A mutation of the mitochondrial ABC transporter Sta1 leads to dwarfism and chlorosis in the Arabidopsis mutant starik
    S Kushnir
    Vakgroep Moleculaire Genetica and Departement Plantengenetica, Vlaams Interuniversitair Instituut voor Biotechnologie VIB, Universiteit Gent, K L Ledeganckstraat 35, B 9000 Gent, Belgium
    Plant Cell 13:89-100. 2001
    ..Taken together, our results suggest that plant mitochondria possess an evolutionarily conserved Fe/S cluster biosynthesis pathway, which is linked to the intracellular iron homeostasis by the function of Atm1p-like ABC transporters...

Research Grants62

  1. ROLE OF B.FRAGILIS OXYGEN STRESS RESPONSE IN INFECTION
    Charles J Smith; Fiscal Year: 2013
    ..We will determine if the iron storage proteins can contribute to the repair of iron sulfur proteins and the in vivo expression of their cognate genes will be measured to gain insight into their role during ..
  2. ROLE OF B.FRAGILIS OXYGEN STRESS RESPONSE IN INFECTION
    Charles Smith; Fiscal Year: 2009
    ..We will determine if the iron storage proteins can contribute to the repair of iron sulfur proteins and the in vivo expression of their cognate genes will be measured to gain insight into their role during ..
  3. NMR SPECTROSCOPY OF IRON-SULFUR PROTEINS
    John L Markley; Fiscal Year: 2010
    ..abstract_text> ..
  4. Assembly of Biological Iron-Sulfur Clusters
    Michael K Johnson; Fiscal Year: 2012
    ..A molecular-level understanding of iron-sulfur cluster biogenesis is crucial for understanding a variety of human diseases involving anemias, myopathies and ataxias that arise from defects in iron-sulfur cluster assembly proteins. ..
  5. COMPUTER SIMULATIONS OF ELECTRON TRANSFER PROTEINS
    Toshiko Ichiye; Fiscal Year: 2013
    ..For instance, dysfunction in respiratory complex I has been associated with human neurodegenerative diseases and aging. ..
  6. MITOCHONDRIAL IRON-SULFUR PROTEINS
    Michael Johnson; Fiscal Year: 1991
    ..In addition they address questions of fundamental importance in the area of biological nitrogen fixation...
  7. STRUCTURAL STUDIES OF ELECTRON TRANSPORT PROTEINS
    Hazel Holden; Fiscal Year: 1992
    ..In addition, what is learned from these bacterial systems will be directly applicable towards understanding the properties of electron-transfer proteins in higher organisms...
  8. Structure and Function of Iron-Sulfur Clusters
    Michael Weaver; Fiscal Year: 2009
    ..Defects in iron-sulfur cluster containing molecules are known to be associated with mitochondrial diseases, and the ability to better model these systems may lend new insight into diseases such as Parkinson's. ..
  9. Tandem TIme-of-Flight Mass Spectromer for Proteomics
    KERMIT MURRAY; Fiscal Year: 2009
    ..The instrument will be operated by skilled technicians, overseen by broadly represented committee and available for use by the entire LSU scientific community. ..
  10. STRUCTURE, FUNCTION AND REACTIVITY--IRON-SULFUR PROTEINS
    BENJAMIN FEINBERG; Fiscal Year: 1992
    ..6. Stereospecific resonance Raman, spectroscopic assignments will be made with specific CyS 13C and 2H derivatives. 7. The influence of specific substitutions on redox and electron transfer capabilities will be examined. ..
  11. Probing the Electronic Structure of Fe-S Clusters
    Lai Sheng Wang; Fiscal Year: 2004
    ..It is expected that the new experimental results from the proposed work will stimulate further theoretical advancements. ..
  12. ELECTRON EXCHANGE EFFECTS ON ELECTRON TRANSFER DYNAMICS
    James McCusker; Fiscal Year: 2000
    ....
  13. THEORETICAL STUDIES OF METALLOPROTEIN CHROMOPHORES
    Richard Friesner; Fiscal Year: 1992
    ..Determination of the factors which control electron transport efficiency and ligand binding and the capacity to predict the effects of chemical modifications on these properties should greatly facilitate the drug design process...
  14. NEW ASPECTS OF STRUCTURE-FUNCTION--IRON-SULFUR PROTEINS
    Helmut Beinert; Fiscal Year: 1992
    ....
  15. KINETIC STUDIES OF HEMERYTHRIN REACTIONS
    RALPH WILKINS; Fiscal Year: 1980
    ..A comparison will be made of these reactions with hemerythrin from other worm species, e.g., Phascolopsis gouldii; mammalian respiratory proteins myoglobin and hemoglobin; and other polynuclear (iron-sulfur) proteins...
  16. RAMAN STUDIES OF SULFITE REDUCTASES AND OTHER ENZYMES
    KWOK YUE; Fiscal Year: 1991
    ..We also propose to characterize the chromophores of other iron- sulfur proteins from sulfate reducing bacteria...
  17. Nitric Oxide Cytotoxicity and Iron-Sulfur Proteins
    Huangen Ding; Fiscal Year: 2009
    ..The proposed research, if successful, will provide fundamental knowledge for developing therapeutic approaches to prevent or alleviate cellular oxidative damages inflicted by NO cytotoxicity. ..
  18. The Physiology of Oxidative Stress in Escherichia coli
    James Imlay; Fiscal Year: 2006
    ..coli will provide a blueprint for efforts to solve key issues in oxidative stress: obligate anaerobiosis, the killing mechanism of) hagocytes, and endogenous oxidative stress in higher organisms. ..
  19. ELECTRONIC STRUCTURE OF IRON-SULFUR AND RELATED PROTEINS
    Louis Noodleman; Fiscal Year: 2010
    ..Calculations and analysis of vibrational spectra and of hyperfine spectra compared with experiment are important indicators of stable intermediates over the catalytic cycle. All changes are in italics. ..
  20. Electronic Structure and Reactions of Fe-oxo Enzymes
    Louis Noodleman; Fiscal Year: 2010
    ..A better understanding of these enzyme mechanisms should aid experimental work toward these goals. ..
  21. CRYSTALLOGRAPHIC STUDIES OF ELECTRON TRANSFER PROTEINS
    Douglas Rees; Fiscal Year: 2002
    ..4. the correspondence between crystallographically defined metallocluster structures and spectroscopically assigned oxidation states in the Fe-protein and MoFe-protein. ..
  22. ENZYME MEDIATED THIOL MODIFICATION OF TRNA
    CHARLES LAUHON; Fiscal Year: 2002
    ....
  23. PROTON & ELECTRON TRANSFER & ENERGY COUPLING IN SITE I
    TOMOKO NONE OHNISHI; Fiscal Year: 2010
    ..Using this new strategy, we found that both cluster N2 and flavin can generate superoxide. We will solidify this exciting finding and study its relevance to complex I function under physiological and pathological conditions. ..
  24. IRON METABOLISM AND PHOSPHORYLATION OF IRPS BY PKC
    RICHARD EISENSTEIN; Fiscal Year: 2002
    ....
  25. MECHANISM OF OXIDATIVE DNA DAMAGE IN MODEL ORGANISMS
    James Imlay; Fiscal Year: 2002
    ..Further, by understanding how metabolic perturbations affect DNA oxidation, we may be able to anticipate the affects of drug therapies upon mutagenesis and cell death. ..
  26. GENETIC CONTROL OF NITRATE RESPIRATION IN E. COLI
    Valley J Stewart; Fiscal Year: 2010
    ..Thus, results from the model species E. coli enhance understanding for a broad range of pathogens that significantly impact public health. ..
  27. MODEL STUDIES OF ACETYL COENZYME A SYNTHASE
    Charles Riordan; Fiscal Year: 2009
    ..More broadly, a deeper understanding of ACS catalysis will advance fundamental understanding of redox-based Ni toxicity. ..
  28. Bioorganic Chemistry of Carbon Monoxide Dehydrogenase
    PAUL LINDAHL; Fiscal Year: 2007
    ..ACS/CODH is involved in C1 metabolism and it contains a tunnel through which CO migrates, impacting the field of metabolic channeling. ..
  29. Repair of Oxidatively Damaged Guanines
    A Lien Lu Chang; Fiscal Year: 2008
    ..We anticipate that these studies will advance our understanding of carcinogenesis process and form the background work for the development of new anti-cancer drugs. ..
  30. FUNCTIONAL STUDIES OF CYTOSOLIC ANTIOXIDANT PROTEINS
    Joan Selverstone Valentine; Fiscal Year: 2010
    ..Together, these studies will shed light on the complex interactions of small molecules and proteins that all eukaryotes use to protect their mitochondria from superoxide-mediated damage. ..
  31. Genetic Analysis of Iron Homeostasis in C.Elegans
    ELIZABETH ANN LEIBOLD; Fiscal Year: 2010
    ..elegans using a green fluorescence protein (GFP)-based screen. ..
  32. Irradiation Damage and Protection of Pulmonary Endothelium Oxidative Lipidomics
    Valerian E Kagan; Fiscal Year: 2010
    ....
  33. Superoxide Generation from eNOS: The Role of Pterins
    JEANNETTE M VASQUEZ VIVAR; Fiscal Year: 2010
    ..Generally this proposal is aimed at understanding these fundamental mechanisms that should provide the basis for developing new and improved strategies in the prevention and treatment of atherosclerosis and CVD. ..
  34. Structure of reaction intermediates in the bc1 complex
    Sergei A Dikanov; Fiscal Year: 2010
    ..The central role of bc1 complex plays out in many medical scenarios in which defects lead to pathology, among them cellular death through ROS-mediated damage, mitochondrial myopathies, and apoptosis, and in drug targeting. ..
  35. Connecting the Genome to the Cytoplasm
    Pamela A Silver; Fiscal Year: 2010
    ..The experiments proposed in this grant will not only lead to new basic insights but also to new ways to analyze on a genome-wide level the situation in genetically defective cells and certain cancers. ..
  36. MECHANISM OF RESPIRATION AND ENERGY TRANSDUCTION
    Bernard Trumpower; Fiscal Year: 2009
    ..abstract_text> ..
  37. Chaperone Function in Iron-Sulfur Protein Folding
    LARRY VICKERY; Fiscal Year: 2008
    ..abstract_text> ..
  38. X-ray studies of metalloenzyme regulation and catalysis
    CATHERINE DRENNAN; Fiscal Year: 2008
    ..We will complement structural data with biochemical characterization and single crystal spectroscopic techniques. ..
  39. PORPHYRAZINE TUMOR CONTRAST AGENTS
    Brian Hoffman; Fiscal Year: 2004
    ..GOAL: To correlate structural modifications of near-it absorbing/emitting pzs with their biological activity, so as to develop optimized, iv-deliverable optical contrast agents. ..
  40. HEME PROTEIN STRUCTURE AND FUNCTION
    Jack Peisach; Fiscal Year: 2004
    ..abstract_text> ..
  41. IRON TRANSPORT IN MAMMALIAN CELLS
    Philip Aisen; Fiscal Year: 2003
    ..Project 5: Structure, function and dynamics in transferrin investigates the molecular mechanisms through which the uptake and release of iron and other metals by transferrin are modulated by physiological factors. ..
  42. COPPER/ALBUMIN REDOX-CYCLING IN PREECLAMPSIA
    Valerian Kagan; Fiscal Year: 2003
    ....
  43. Kinetic Simulations of Minimal Living Systems
    PAUL LINDAHL; Fiscal Year: 2002
    ..Simulation and animations will be critically compared to the properties of real cells. Mechanism will be modified, corrected, and embellished as deficiencies are noted and more experimental data become available. ..
  44. Gordon Research Conference on Tetrapyrroles
    Gloria Ferreira; Fiscal Year: 2002
    ..Strengths of this ongoing Gordon conference are its disciplinary diversity, focus on new unpublished work, time for discussion, informal atmosphere, and ban on dissemination of conference proceeding. ..
  45. Bruker Q-Band CW EPR Spectrometer
    Brian Hoffman; Fiscal Year: 2002
    ..This must be replaced, and funds are requested for the purchase of a Bruker EMX 13/15 Q-band EP0R spectrometer with Q- band bridge and related equipment. This will be mated to the CW ENDOR apparatus in current use. ..
  46. REGULATION OF E COLI CYTOCHROME OXIDASE AND TCA CYCLE
    Robert Gunsalus; Fiscal Year: 2001
    ..coli. These studies should provide a detailed understanding of how the cell coordinates pathways for electron and carbon flow within the cell during transition between oxygen rich and oxygen limiting conditions. ..
  47. OXYGEN SENSING AND CELL SIGNALING IN HYPOXIA
    Paul Schumacker; Fiscal Year: 2001
    ..These studies will identify a novel pathway of cellular O2 detection, and may help clarify understanding of how cells adapt to lowered O2 conditions. ..
  48. SUMMER RESEARCH IN MACROMOLECULAR INTERACTIONS
    CATHERINE DRENNAN; Fiscal Year: 2004
    ..The proposed training project complements institutional goals of expanding career choices for students majoring in physical and quantitative sciences, and increasing minority participation in undergraduate research programs. ..
  49. THE HISTIDYL IMIDAZOLE LIGAND IN METALLOPROTEINS
    JOAN VALENTINE; Fiscal Year: 2005
    ....
  50. MECHANISTIC STUDIES OF DNA MISMATCH REPAIR
    A Lien Lu Chang; Fiscal Year: 2006
    ..Particularly, alterations in protein-protein interactions under oxidative stress will be investigated. These studies should advance our understanding of the role of DNA repair in genome stability and tumor susceptibility. ..
  51. ANAEROBIC EXPRESSION OF FUMARATE REDUCTASE IN E. COLI
    Robert Gunsalus; Fiscal Year: 2008
    ..The Nar and Mode gene families will be identified by microarray analysis. Finally, the physiology of nitrate control will be examined by use of continuous culture methods. [unreadable] [unreadable]..
  52. Tetrahydrobiopterin in Hypoxia-Ischemia Induced Fetal Brain Dysfunction
    Jeannette Vasquez Vivar; Fiscal Year: 2008
    ..This information will likely define the use of tetrahydrobiopterin in the prevention and/or amelioration of H-I induced fetal brain dysfunction, which remains a major medical challenge. [unreadable] [unreadable] [unreadable]..
  53. Structure-Function of Hetero-oligomeric Integral Membrane Proteins
    WILLIAM CRAMER; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  54. Role of Shigella two component regulation systems in intracellular adaptation
    Laura Runyen Janecky; Fiscal Year: 2007
    ..The research described in this proposal will be useful for designing more effective therapies and will yield information applicable to other similar pathogens. [unreadable] [unreadable] [unreadable] [unreadable]..
  55. Receptor-mediated Colicin Import
    WILLIAM CRAMER; Fiscal Year: 2007
    ..2) The pathway of membrane insertion of the E1 channel domain, relative to the time course of other unfolding events, will be examined by time-resolved FRET using a large set of single Trp mutants. [unreadable] [unreadable]..
  56. Structures of Bacterial Membrane-Bound Oxidoreductases
    Joel Weiner; Fiscal Year: 2006
    ....
  57. Directed evolution of P450 peroxide-mediated activity
    JONATHAN SILBERG; Fiscal Year: 2004
    ....
  58. MECHANISMS OF ENZYMATIC REACTIONS
    PERRY FREY; Fiscal Year: 2006
    ..unreadable] [unreadable]..
  59. ENZYME CATALYSIS OF ELECTRON AND GROUP TRANSFER
    PERRY FREY; Fiscal Year: 2006
    ..The contributions of beta- amino acids produced by lysine 2,3-aminomutase and arginine 2,3-aminomutase to the functions of antibiotics are not known. The beta-aminoacids may become significant in drug development. ..
  60. IRON BINDING PROTEINS AND CONTROL OF IRON METABOLISM
    Philip Aisen; Fiscal Year: 2001
    ..Understanding the fundamental chemistry of iron transport, storage and utilization will provide the rational basis for managing the multitude of diseases where aberrations of iron metabolism contribute to cellular injury and death. ..