peptide elongation factor 1

Summary

Summary: Peptide elongation factor 1 is a multisubunit protein that is responsible for the GTP-dependent binding of aminoacyl-tRNAs to eukaryotic ribosomes. The alpha subunit (EF-1alpha) binds aminoacyl-tRNA and transfers it to the ribosome in a process linked to GTP hydrolysis. The beta and delta subunits (EF-1beta, EF-1delta) are involved in exchanging GDP for GTP. The gamma subunit (EF-1gamma) is a structural component.

Top Publications

  1. ncbi Validation of zebrafish (Danio rerio) reference genes for quantitative real-time RT-PCR normalization
    Rongying Tang
    School of Biological Sciences, University of Auckland, Private Bag 92019, Auckland 1142, New Zealand
    Acta Biochim Biophys Sin (Shanghai) 39:384-90. 2007
  2. ncbi RNA-mediated response to heat shock in mammalian cells
    Ilya Shamovsky
    Department of Biochemistry, New York University School of Medicine, New York, New York 10016, USA
    Nature 440:556-60. 2006
  3. ncbi Reference gene selection for quantitative real-time PCR normalization in tomato subjected to nitrogen, cold, and light stress
    Trond Løvdal
    Faculty of Science and Technology, Centre for Organelle Research, University of Stavanger, N 4036 Stavanger, Norway
    Anal Biochem 387:238-42. 2009
  4. pmc Exp5 exports eEF1A via tRNA from nuclei and synergizes with other transport pathways to confine translation to the cytoplasm
    Markus T Bohnsack
    ZMBH, INF 282, D 69120 Heidelberg, Germany
    EMBO J 21:6205-15. 2002
  5. ncbi Novel complexes of mammalian translation elongation factor eEF1A.GDP with uncharged tRNA and aminoacyl-tRNA synthetase. Implications for tRNA channeling
    Zoya M Petrushenko
    Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, Kiev, Ukraine
    Eur J Biochem 269:4811-8. 2002
  6. pmc The elongation factor 1 A-2 isoform from rabbit: cloning of the cDNA and characterization of the protein
    S Kahns
    Division of Biostructural Chemistry, IMSB, Aarhus University, Gustav Wieds Vej 10C, 8000 Arhus C, Denmark
    Nucleic Acids Res 26:1884-90. 1998
  7. pmc Evidence that eukaryotic translation elongation factor 1A (eEF1A) binds the Gcn2 protein C terminus and inhibits Gcn2 activity
    Jyothsna Visweswaraiah
    Institute of Natural Sciences, Massey University, Auckland 0745, New Zealand
    J Biol Chem 286:36568-79. 2011
  8. ncbi Phylogeny and toxigenic potential is correlated in Fusarium species as revealed by partial translation elongation factor 1 alpha gene sequences
    Ralf Kristensen
    Section of Food and Feed Microbiology, National Veterinary Institute, N 0033 Oslo, Norway
    Mycol Res 109:173-86. 2005
  9. pmc A calmodulin-sensitive interaction between microtubules and a higher plant homolog of elongation factor-1 alpha
    N A Durso
    Department of Biology, Pennsylvania State University, University Park 16802
    Plant Cell 6:893-905. 1994
  10. ncbi The 'Expansion-Contraction' model of Pleistocene biogeography: rocky shores suffer a sea change?
    Peter B Marko
    Department of Biological Sciences, Clemson University, Clemson, SC 29634 0314, USA
    Mol Ecol 19:146-69. 2010

Research Grants

  1. GENE EXPRESSION OF NEGATIVE STRAND RNA VIRUSES
    Amiya Banerjee; Fiscal Year: 2009
  2. GENE STRUCTURE AND EF-1A IN THE FUNGUS MUCOR
    PAUL SYPHERD; Fiscal Year: 1991
  3. Spontaneous Lytic Replication of KSHV
    Jeffrey Vieira; Fiscal Year: 2008
  4. Molecular genetics of translational accuracy
    Philip Farabaugh; Fiscal Year: 2007
  5. Arp2/3 mRNA localization and directional cell migration
    Gang Liu; Fiscal Year: 2008
  6. SIGNALING COMPLEXES AND THE 14-3-3 PROTEIN IN CANDIDA
    Joy Sturtevant; Fiscal Year: 2008
  7. Development of selenomethionine resistant yeast
    TERRI KINZY; Fiscal Year: 2007
  8. Vacuole expansion during flamentation in Candida
    Joy Sturtevant; Fiscal Year: 2006
  9. Targeting of somatic hypermutation and gene conversion
    David Schatz; Fiscal Year: 2006
  10. Discovery of New Cellular Targets of Anthrax Toxin
    ALINE SCANDURRO; Fiscal Year: 2004

Detail Information

Publications262 found, 100 shown here

  1. ncbi Validation of zebrafish (Danio rerio) reference genes for quantitative real-time RT-PCR normalization
    Rongying Tang
    School of Biological Sciences, University of Auckland, Private Bag 92019, Auckland 1142, New Zealand
    Acta Biochim Biophys Sin (Shanghai) 39:384-90. 2007
    ..Importantly, the zebrafish GAPDH gene appears unsuitable as reference gene for both types of studies...
  2. ncbi RNA-mediated response to heat shock in mammalian cells
    Ilya Shamovsky
    Department of Biochemistry, New York University School of Medicine, New York, New York 10016, USA
    Nature 440:556-60. 2006
    ..The central role of HSR1 during heat shock implies that targeting this RNA could serve as a new therapeutic model for cancer, inflammation and other conditions associated with HSF1 deregulation...
  3. ncbi Reference gene selection for quantitative real-time PCR normalization in tomato subjected to nitrogen, cold, and light stress
    Trond Løvdal
    Faculty of Science and Technology, Centre for Organelle Research, University of Stavanger, N 4036 Stavanger, Norway
    Anal Biochem 387:238-42. 2009
    ..Thus, the results highlight the need for normalizing gene expression in tomato using the geometric average of multiple carefully selected reference genes...
  4. pmc Exp5 exports eEF1A via tRNA from nuclei and synergizes with other transport pathways to confine translation to the cytoplasm
    Markus T Bohnsack
    ZMBH, INF 282, D 69120 Heidelberg, Germany
    EMBO J 21:6205-15. 2002
    ..Besides Exp5 and importin 13, CRM1 and as yet unidentified exportins also contribute to the depletion of translation factors from nuclei...
  5. ncbi Novel complexes of mammalian translation elongation factor eEF1A.GDP with uncharged tRNA and aminoacyl-tRNA synthetase. Implications for tRNA channeling
    Zoya M Petrushenko
    Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, Kiev, Ukraine
    Eur J Biochem 269:4811-8. 2002
    ..However, the addition of tRNAPhe accelerated eEF1A.GDP binding to the enzyme. A possible role of these stable novel ternary and quaternary complexes of eEF1A.GDP with tRNA and ARS in the channeled elongation cycle is discussed...
  6. pmc The elongation factor 1 A-2 isoform from rabbit: cloning of the cDNA and characterization of the protein
    S Kahns
    Division of Biostructural Chemistry, IMSB, Aarhus University, Gustav Wieds Vej 10C, 8000 Arhus C, Denmark
    Nucleic Acids Res 26:1884-90. 1998
    ..In contrast, the GDP dissociation rate constant is approximately 7 times higher for eEF1A-1 than for eEF1A-2. The nucleotide preference ratio (GDP/GTP) for eEF1A-1 was 0.82, while the preference ratio for eEF1A-2 was 1.50...
  7. pmc Evidence that eukaryotic translation elongation factor 1A (eEF1A) binds the Gcn2 protein C terminus and inhibits Gcn2 activity
    Jyothsna Visweswaraiah
    Institute of Natural Sciences, Massey University, Auckland 0745, New Zealand
    J Biol Chem 286:36568-79. 2011
    ..These findings implicate eEF1A in the intricate regulation of Gcn2 and amino acid homeostasis...
  8. ncbi Phylogeny and toxigenic potential is correlated in Fusarium species as revealed by partial translation elongation factor 1 alpha gene sequences
    Ralf Kristensen
    Section of Food and Feed Microbiology, National Veterinary Institute, N 0033 Oslo, Norway
    Mycol Res 109:173-86. 2005
    ..Implications for a better classification of Fusarium are discussed and highlighted...
  9. pmc A calmodulin-sensitive interaction between microtubules and a higher plant homolog of elongation factor-1 alpha
    N A Durso
    Department of Biology, Pennsylvania State University, University Park 16802
    Plant Cell 6:893-905. 1994
    ..Based on these findings, and others discussed herein, we propose that an EF-1 alpha homolog mediates the lateral association of MTs in plant cells by a Ca2+/CaM-sensitive mechanism...
  10. ncbi The 'Expansion-Contraction' model of Pleistocene biogeography: rocky shores suffer a sea change?
    Peter B Marko
    Department of Biological Sciences, Clemson University, Clemson, SC 29634 0314, USA
    Mol Ecol 19:146-69. 2010
    ..Our results indicate that the LGM did not extirpate the majority of species in the northeastern Pacific; instead, regional persistence during the LGM appears a common biogeographic history for rocky-shore organisms in this region...
  11. pmc A critical role for eukaryotic elongation factor 1A-1 in lipotoxic cell death
    Nica M Borradaile
    Center for Cardiovascular Research, Division of Cardiology, Department of Internal Medicine, Washington University School of Medicine, St Louis, MO 63110, USA
    Mol Biol Cell 17:770-8. 2006
    ..Furthermore, our observations of oxidative stress, ER stress, and induction of eEF1A-1 expression in a mouse model of lipotoxic cardiomyopathy implicate this cellular response in the pathophysiology of metabolic disease...
  12. ncbi BPOZ-2 directly binds to eEF1A1 to promote eEF1A1 ubiquitylation and degradation and prevent translation
    Kotaro Koiwai
    Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, Noda, Chiba, Japan
    Genes Cells 13:593-607. 2008
    ..BPOZ-2 promoted eEF1A1 ubiquitylation and degradation, suggesting that eEF1A1 is a substrate of BPOZ-2. BPOZ-2 inhibited GTP binding to eEF1A1 and prevented translation in in vitro translation assay using rabbit reticulocytes...
  13. ncbi Comparison of predictive methods and biological validation for qPCR reference genes in sunflower leaf senescence transcript analysis
    Paula Fernandez
    Instituto de Biotecnologia, CICVyA, INTA Castelar, Las Cabañas y Los Reseros, 1686 Hurlingham, Buenos Aires, Argentina
    Plant Cell Rep 30:63-74. 2011
    ....
  14. pmc Structural basis for mRNA surveillance by archaeal Pelota and GTP-bound EF1α complex
    Kan Kobayashi
    Division of Structure Biology, Department of Basic Medical Sciences, The Institute of Medical Science, University of Tokyo, 4 6 1 Shirokanedai, Minato ku, Tokyo 108 8639, Japan
    Proc Natl Acad Sci U S A 107:17575-9. 2010
    ..Our findings suggest that the molecular mimicry of tRNA in the distorted "A/T state" conformation by Pelota enables the complex to efficiently detect and enter the empty A site of the stalled ribosome...
  15. ncbi The translation elongation factor 1A in tumorigenesis, signal transduction and apoptosis: review article
    A Lamberti
    Dipartimento di Biochimica e Biotecnologie Mediche, Universita di Napoli Federico II, Naples, Italy
    Amino Acids 26:443-8. 2004
    ..This article summarizes the present knowledges on the several extra-translational roles of eEF1A also in order to understand as the protein synthesis regulatory mechanisms could offer tools for cancer intervention...
  16. ncbi Translation elongation factor eEF1A2 is essential for post-weaning survival in mice
    H J Newbery
    Medical Genetics, Molecular Medicine Centre, University of Edinburgh, Western General Hospital, Edinburgh EH4 2XU, United Kingdom
    J Biol Chem 282:28951-9. 2007
    ..We conclude that loss of eEF1A2 function is solely responsible for the abnormalities seen in these mice...
  17. pmc Molecular phylogenetic diversity, multilocus haplotype nomenclature, and in vitro antifungal resistance within the Fusarium solani species complex
    Kerry O'Donnell
    Microbial Genomics and Bioprocessing Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, U S Department of Agriculture, 1815 North University Street, Peoria, IL 61604 3999, USA
    J Clin Microbiol 46:2477-90. 2008
    ..The in vitro activities of 10 antifungals tested against 19 isolates representing 18 species that span the breadth of the FSSC phylogeny show that members of this complex are broadly resistant to these drugs...
  18. ncbi A Beauveria phylogeny inferred from nuclear ITS and EF1-alpha sequences: evidence for cryptic diversification and links to Cordyceps teleomorphs
    Stephen A Rehner
    Insect Biocontrol Laboratory, USDA ARS, Beltsville, Maryland 20705, USA
    Mycologia 97:84-98. 2005
    ..These results establish a phylogenetic framework for further taxonomic, phylogenetic and comparative biological investigations of Beauveria and their corresponding Cordyceps teleomorphs...
  19. pmc eEF1A is a novel component of the mammalian nuclear protein export machinery
    Mireille Khacho
    Department of Cellular and Molecular Medicine, Faculty of Medicine, University of Ottawa, Ottawa, Ontario, K1H 8M5, Canada
    Mol Biol Cell 19:5296-308. 2008
    ..These results also provide a link between the translational apparatus and subcellular trafficking machinery demonstrating that these two central pathways in basic metabolism can act cooperatively...
  20. ncbi Promoter-dependent EGFP expression during embryonic stem cell propagation and differentiation
    Rong Wang
    Key Laboratory of Stem Cell Biology of Institute of Health Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China
    Stem Cells Dev 17:279-89. 2008
    ..These results extend previous observations for the activities of these promoters in mES cells and provide new information for choosing appropriate promoters to facilitate genetic modification of mES cells...
  21. pmc Interactions of elongation factor 1alpha with F-actin and beta-actin mRNA: implications for anchoring mRNA in cell protrusions
    Gang Liu
    Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, New York, New York 10461, USA
    Mol Biol Cell 13:579-92. 2002
    ..Taken together, these results implicate EF1alpha in the anchoring of beta-actin mRNA to the protrusion in crawling cells...
  22. ncbi Novel cell death by downregulation of eEF1A1 expression in tetraploids
    Y Kobayashi
    Laboratory of Molecular and Cellular Biology, Department of Animal Development and Physiology, Graduate School of Biostudies, Kyoto University, Kyoto 606 8501, Japan
    Cell Death Differ 16:139-50. 2009
    ..Taken together, the novel cell death machinery should help to eliminate abnormal tetraploid cells and inhibit tumorigenesis...
  23. ncbi A kingdom-level phylogeny of eukaryotes based on combined protein data
    S L Baldauf
    Department of Biology, University of York, Heslington, York, YO10 5DD, UK
    Science 290:972-7. 2000
    ..The resulting phylogenetic tree shows a number of striking differences from SSU rRNA phylogeny, including strong support for most major groups and several major supergroups...
  24. pmc Structural models of human eEF1A1 and eEF1A2 reveal two distinct surface clusters of sequence variation and potential differences in phosphorylation
    Dinesh C Soares
    Medical Genetics Section, Molecular Medicine Centre, Institute of Genetics and Molecular Medicine, Western General Hospital, University of Edinburgh, Edinburgh, United Kingdom
    PLoS ONE 4:e6315. 2009
    ..This may reflect the differential requirements of the cell-types in which they are expressed and is consistent with complex roles for these proteins that extend beyond delivery of tRNA to the ribosome...
  25. ncbi The prognostic significance of elongation factor eEF1A2 in ovarian cancer
    Dixie E Pinke
    Department of Biochemistry, Microbiology, Immunology, University of Ottawa, 451 Smyth Road, Ottawa, Ontario, Canada K1H 8M5
    Gynecol Oncol 108:561-8. 2008
    ..To determine whether eukaryotic elongation factor 1 alpha 2 (eEF1A2), a transforming gene previously shown to be highly expressed in primary human ovarian tumours, is a prognostic marker...
  26. ncbi The lipid kinase PI4KIIIβ and the eEF1A2 oncogene co-operate to disrupt three-dimensional in vitro acinar morphogenesis
    Dixie E Pinke
    Department of Biochemistry, University of Ottawa, 451 Smyth Road, Ottawa, Ontario, Canada K1H 8M5
    Exp Cell Res 317:2503-11. 2011
    ..Our work shows that PI4KIIIβ, eEF1A2 and PI(4)P likely play an important role in mammary neoplasia and acinar development...
  27. ncbi Characterization of elongation factor-1A (eEF1A-1) and eEF1A-2/S1 protein expression in normal and wasted mice
    A Khalyfa
    Bloomfield Center for Research in Aging, Lady Davis Institute for Medical Research, The Sir Mortimer B Davis Jewish General Hospital, McGill University, Montreal, Quebec H3T 1E2, Canada
    J Biol Chem 276:22915-22. 2001
    ....
  28. pmc Eef1a2 promotes cell growth, inhibits apoptosis and activates JAK/STAT and AKT signaling in mouse plasmacytomas
    Zhaoyang Li
    Laboratory of Immunopathology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, Maryland, USA
    PLoS ONE 5:e10755. 2010
    ..High-level expression was also a feature of a subset of cell lines developed from mouse PCT and from the human MM...
  29. ncbi Unique modifications of translation elongation factors
    Eva Greganova
    Institute for Biochemistry and Molecular Medicine, University of Berne, Berne, Switzerland
    FEBS J 278:2613-24. 2011
    ..We review the biosynthesis, attachment and physiological roles of these unique protein modifications and discuss common and separate features of the target proteins, which represent essential proteins in all organisms...
  30. ncbi Biological characterization of various forms of elongation factor 1 from rabbit reticulocytes
    M D Carvalho
    Arch Biochem Biophys 234:603-11. 1984
    ..The results presented in this report, along with studies from other laboratories, strengthen the hypothesis that the general mechanism of the elongation cycle is similar in eucaryotes and procaryotes...
  31. ncbi Validation of reference genes for transcription profiling in the salmon louse, Lepeophtheirus salmonis, by quantitative real-time PCR
    Petter Frost
    Department of Aquaculture, Institute of Marine Research, P O Box 1870, Nordnes, N 5817 Bergen, Norway
    Vet Parasitol 118:169-74. 2003
    ..salmonis life cycle. Furthermore our results indicated that 18S RNA, although constitutively expressed, is not a convenient reference gene for relative quantification of most transcripts...
  32. pmc Tumor necrosis factor-alpha downregulates endothelial nitric oxide synthase mRNA stability via translation elongation factor 1-alpha 1
    Guijun Yan
    Department of Cell Biology and Molecular Medicine, UMDNJ New Jersey Medical School, Newark, NJ 07103, USA
    Circ Res 103:591-7. 2008
    ..These results indicate that eEF1A1 is a novel eNOS 3'-UTR binding protein that plays a critical role in mediating TNF-alpha-induced decrease in eNOS mRNA stability...
  33. ncbi Phylogeography of the red coral (Corallium rubrum): inferences on the evolutionary history of a temperate gorgonian
    D Aurelle
    CNRS UMR 6540 DIMAR, Centre d Oceanologie de Marseille, Aix Marseille Universite, Station marine d Endoume, rue de la Batterie des Lions, 13007 Marseille, France
    Genetica 139:855-69. 2011
    ..Our results suggest that recent Quaternary environmental fluctuations had a limited impact on the genetic structure of C. rubrum...
  34. ncbi A PCR-denaturing gradient gel electrophoresis approach to assess Fusarium diversity in asparagus
    E Yergeau
    Institut de recherche en biologie végétale, Université de Montréal and Jardin botanique de Montréal, 4101 East, Sherbrooke Street, Montreal, QC, Canada H1X 2B2
    J Microbiol Methods 60:143-54. 2005
    ..oxysporum, F. proliferatum or F. solani, clearly supporting the reliability, sensitivity and specificity of this approach for the study of Fusarium diversity from asparagus plants samples...
  35. pmc Coordination of eukaryotic translation elongation factor 1A (eEF1A) function in actin organization and translation elongation by the guanine nucleotide exchange factor eEF1Balpha
    Yvette R Pittman
    Department of Molecular Genetics, Microbiology, and Immunology, University of Medicine and Dentistry of New Jersey Robert Wood Johnson Medical School, Piscataway, New Jersey 08854, USA
    J Biol Chem 284:4739-47. 2009
    ..The consequences of the overlapping functions in this eEF1A domain and its unique differences from the bacterial homologs provide a novel function for eEF1Balpha to balance the dual roles in actin bundling and protein synthesis...
  36. ncbi The role of translation elongation factor eEF1A in intracellular alkalinization-induced tumor cell growth
    Juno Kim
    Department of Pharmacology and Brain Korea 21 Project for Medical Science, Yonsei University College of Medicine, Seoul, Korea
    Lab Invest 89:867-74. 2009
    ..The above findings suggest that an increase in free-form eEF1A under alkaline conditions plays a critical role in alkalinization-induced cell growth...
  37. pmc Inhibition of proliferation, invasion, and migration of prostate cancer cells by downregulating elongation factor-1alpha expression
    Gang Zhu
    Department of Urology, Beijing Hospital MH, Beijing, China
    Mol Med 15:363-70. 2009
    ..001). Taken together, these findings support the hypothesis that EF-1alpha affects multiple processes involved in tumor progression, and identify EF-1alpha as a potential therapeutic target...
  38. ncbi Tissue-dependent variation in the expression of elongation factor-1 alpha isoforms: isolation and characterisation of a cDNA encoding a novel variant of human elongation-factor 1 alpha
    S M Knudsen
    Department of Biostructural Chemistry, Kemisk Institut, Aarhus, Denmark
    Eur J Biochem 215:549-54. 1993
    ..EF-1 alpha was expressed in brain, placenta, lung, liver, kidney, pancreas and in all the cell lines that we have analysed but barely detectable in heart and skeletal muscle...
  39. pmc Molecular mechanism of elongation factor 1A inhibition by a Legionella pneumophila glycosyltransferase
    Ramon Hurtado-Guerrero
    Division of Molecular Microbiology, College of Life Sciences, University of Dundee, Dundee DD1 5EH, Scotland, UK
    Biochem J 426:281-92. 2010
    ....
  40. pmc eEF1A isoforms change in abundance and actin-binding activity during maize endosperm development
    Jose A Lopez-Valenzuela
    Department of Plant Sciences, University of Arizona, Tucson, Arizona 85721, USA
    Plant Physiol 133:1285-95. 2003
    ..The chemical differences that account for their different actin-binding activities could not be determined...
  41. pmc Two novel methyltransferases acting upon eukaryotic elongation factor 1A in Saccharomyces cerevisiae
    Rebecca S Lipson
    Department of Chemistry and Biochemistry, Molecular Biology Institute, UCLA, Los Angeles, CA 90095 1569, USA
    Arch Biochem Biophys 500:137-43. 2010
    ..We suggest that YHL039W (now designated EFM1 for elongation factor methyltransferase 1) and YIL064W/SEE1 encode distinct eEF1A methyltransferases that respectively monomethylate and dimethylate this protein at lysine residues...
  42. ncbi Location of seven post-translational modifications in rabbit elongation factor 1 alpha including dimethyllysine, trimethyllysine, and glycerylphosphorylethanolamine
    T E Dever
    Department of Biochemistry, Case Western Reserve University School of Medicine, Cleveland, Ohio 44106
    J Biol Chem 264:20518-25. 1989
    ..Therefore, it seems likely that the modifying enzymes do not recognize a specific amino acid sequence but rather the three-dimensional presentation of either amino or carboxyl residues in the elongation factor 1 alpha structure...
  43. ncbi Molecular characterization and expression analysis of nine cotton GhEF1A genes encoding translation elongation factor 1A
    Wen Liang Xu
    College of Life Sciences, Huazhong Normal University, Wuhan 430079, P R China
    Gene 389:27-35. 2007
    ..Further analysis indicated that expressions of the GhEF1As in fiber are highly developmental-regulated, suggesting that protein biosynthesis is very active at the early fiber elongation...
  44. ncbi The NS5A protein of bovine viral diarrhoea virus interacts with the alpha subunit of translation elongation factor-1
    C M Johnson
    Department of Veterinary and Biomedical Sciences (202 VBS, University of Nebraska-Lincoln, Fair Street and East Campus Loop, Lincoln, NE 68583-0905, USA
    J Gen Virol 82:2935-43. 2001
    ..The conserved association of eEF1A with virus molecules involved in genome replication and the postulated role of pestivirus and hepacivirus NS5A in replication indicate that this interaction may play a role in the replication of BVDV...
  45. ncbi The hepatitis delta virus RNA genome interacts with eEF1A1, p54(nrb), hnRNP-L, GAPDH and ASF/SF2
    Dorota Sikora
    Department of Biochemistry, Microbiology and Immunology, Faculty of Medicine, University of Ottawa, 451 Smyth Road, Room 4111A, Ottawa, Ontario, Canada, K1H 8M5
    Virology 390:71-8. 2009
    ..Our discovery that HDV RNA associates with RNA-processing pathways and translation machinery during its replication provides new insights into HDV biology and its pathogenicity...
  46. ncbi Resveratrol suppresses growth of human ovarian cancer cells in culture and in a murine xenograft model: eukaryotic elongation factor 1A2 as a potential target
    Mee Hyun Lee
    Department of Pharmacy, College of Pharmacy, and Cancer Research Institute, Seoul National University, Seoul, South Korea
    Cancer Res 69:7449-58. 2009
    ..Taken together, eEF1A2 may be considered as a potential molecular target for the antiproliferative effects of resveratrol in PA-1 ovarian cancer cells...
  47. ncbi A two-locus DNA sequence database for typing plant and human pathogens within the Fusarium oxysporum species complex
    Kerry O'Donnell
    Microbial Genomics Research Unit, Agricultural Research Service, US Department of Agriculture, Peoria, IL 61604, USA
    Fungal Genet Biol 46:936-48. 2009
    ..We also evaluated enniatin, fumonisin and moniliformin mycotoxin production in vitro within a phylogenetic framework...
  48. pmc Elongation Factor 1 alpha interacts with phospho-Akt in breast cancer cells and regulates their proliferation, survival and motility
    Luisa Pecorari
    Department of Biomedical Sciences, University of Modena and Reggio Emilia, 41100 Modena, Italy
    Mol Cancer 8:58. 2009
    ..This study was undertaken to identify pAkt-interacting proteins and to assess their biological roles in breast cancer cells...
  49. pmc A self-inactivating lentiviral vector for SCID-X1 gene therapy that does not activate LMO2 expression in human T cells
    Sheng Zhou
    Division of Experimental Hematology, Department of Hematology, St Jude Children s Research Hospital, 262 Danny Thomas Pl, Memphis, TN 38105, USA
    Blood 116:900-8. 2010
    ..We conclude that the CL20i4-EF1alpha-hgamma(c)OPT vector may be suitable for testing in a clinical trial based on these preclinical demonstrations of efficacy and safety...
  50. ncbi Regulation and functional role of eEF1A2 in pancreatic carcinoma
    Haixia Cao
    Department of Gastroenterology, Rui Jin Hospital, Shanghai Jiaotong University School of Medicine, No 197 Rui Jin Er Road, Shanghai 200025, China
    Biochem Biophys Res Commun 380:11-6. 2009
    ..Our data thus suggest that eEF1A2 might play an important role in pancreatic carcinogenesis, possibly by acting as a tumour oncogene...
  51. pmc Kinetics of the interactions between yeast elongation factors 1A and 1Balpha, guanine nucleotides, and aminoacyl-tRNA
    Kirill B Gromadski
    Institute of Physical Biochemistry, University of Witten Herdecke, Stockumer Strasse 10, D 58448 Witten, Germany
    J Biol Chem 282:35629-37. 2007
    ..eEF1A.GTP binds Phe-tRNA(Phe) with a K(d) of 3 nm, whereas eEF1A.GDP shows no significant binding, indicating that eEF1A has similar tRNA binding properties as its prokaryotic homolog, EF-Tu...
  52. ncbi Efficiency of the elongation factor-1alpha promoter in mammalian embryonic stem cells using lentiviral gene delivery systems
    Sinae Kim
    Stem Cell Research Laboratory, CHA Stem Cell Institute, Pochon CHA University, Seoul 135 081, Korea
    Stem Cells Dev 16:537-45. 2007
    ..These results imply that genetic modification by lentiviral vectors with specific promoters in ES cells constitute a powerful tool for guided differentiation as well as gene therapy...
  53. pmc The Opitz syndrome gene product MID1 assembles a microtubule-associated ribonucleoprotein complex
    Beatriz Aranda-Orgilles
    Max Planck Institute for Molecular Genetics, Ihnestr 73, 14195, Berlin, Germany
    Hum Genet 123:163-76. 2008
    ..The dysfunction of this mechanism would lead to malfunction of microtubule-associated protein translation and to the development of OS...
  54. pmc Multiple evolutionary origins of the fungus causing Panama disease of banana: concordant evidence from nuclear and mitochondrial gene genealogies
    K O'Donnell
    National Center for Agricultural Utilization Research, U S Department of Agriculture Agricultural Research Service, 1815 North University Street, Peoria, IL 61604, USA
    Proc Natl Acad Sci U S A 95:2044-9. 1998
    ..Taken together, these results indicate Panama disease of banana is caused by fungi with independent evolutionary origins...
  55. ncbi Phylogenetic relationships among species of Pichia, Issatchenkia and Williopsis determined from multigene sequence analysis, and the proposal of Barnettozyma gen. nov., Lindnera gen. nov. and Wickerhamomyces gen. nov
    Cletus P Kurtzman
    Microbial Genomics and Bioprocessing Research Unit, National Center for Agricultural Utilization Research, US Department of Agriculture, Agricultural Research Service, Peoria, IL 61604, USA
    FEMS Yeast Res 8:939-54. 2008
    ..Lineages previously classified as varieties of Pichia kluyveri, 'Issatchenkia'scutulata, Starmera amethionina and 'Williopsis'saturnus are elevated to species rank based on sequence comparisons...
  56. pmc Omnipotent role of archaeal elongation factor 1 alpha (EF1α in translational elongation and termination, and quality control of protein synthesis
    Kazuki Saito
    Division of Molecular Biology, Department of Basic Medical Science, Institute of Medical Science, University of Tokyo, 4 6 1 Shirokanedai, Minato ku, Tokyo 108 8639, Japan
    Proc Natl Acad Sci U S A 107:19242-7. 2010
    ....
  57. ncbi Tetrahymena eukaryotic translation elongation factor 1A (eEF1A) bundles filamentous actin through dimer formation
    Fumihide Bunai
    Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki 305 8572
    J Biochem 140:393-9. 2006
    ..Simultaneously, Ca2+/CaM/ eEF1A monomer complexes were dissociated from actin filaments. Therefore, Ca2+/CaM reversibly regulates the F-actin bundling activity of eEF1A...
  58. ncbi Extended conformation of mammalian translation elongation factor 1A in solution
    T V Budkevich
    Institute of Molecular Biology and Genetics, National Academy of Sciences, 150 Zabolotnogo Street, Kiev, 03143 Ukraine
    Biochemistry 41:15342-9. 2002
    ....
  59. pmc Clinical significance of elongation factor-1 delta mRNA expression in oesophageal carcinoma
    K Ogawa
    Department of Molecular and Surgical Oncology, Medical Institute of Bioregulation, Kyushu University, Tsurumihara 4546, Beppu 874 0838, Japan
    Br J Cancer 91:282-6. 2004
    ....
  60. ncbi A five-gene phylogeny of Pezizomycotina
    Joseph W Spatafora
    Department of Botany and Plant Pathology, Oregon State University, Corvallis 97331, USA
    Mycologia 98:1018-28. 2006
    ..Within Leotiomyceta, the supraclass clades of Leotiomycetes s.s. plus Sordariomycetes and Arthoniomycetes plus Dothideomycetes were resolved with moderate support...
  61. pmc Txk, a member of the non-receptor tyrosine kinase of the Tec family, forms a complex with poly(ADP-ribose) polymerase 1 and elongation factor 1alpha and regulates interferon-gamma gene transcription in Th1 cells
    T Maruyama
    Departments of Immunology and Medicine, St Marianna University School of Medicine, Kawasaki, Japan
    Clin Exp Immunol 147:164-75. 2007
    ..Collectively, Txk in combination with EF-1alpha and PARP1 bound to the IFN-gamma gene promoter, and exerted transcriptional activity on the IFN-gamma gene...
  62. ncbi Monoclonal antibodies to elongation factor-1alpha inhibit in vitro translation in lysates of Sf21 cells
    M K Stuart
    Department of Microbiology Immunology, Kirksville College of Osteopathic Medicine, Kirksville, Missouri 63501, USA
    Arch Insect Biochem Physiol 52:17-34. 2003
    ..Of the four EF-1alpha-specific MAbs tested, three significantly inhibited protein synthesis when compared to the negative control antibody (P < 0.001, one-way ANOVA; followed by Dunnett's test, P < 0.05)...
  63. ncbi eEF1A2 activates Akt and stimulates Akt-dependent actin remodeling, invasion and migration
    A Amiri
    Department of Biochemistry, Microbiology and Immunology, University of Ottawa, Ottawa, Ontario, Canada
    Oncogene 26:3027-40. 2007
    ..These results suggest that eEF1A2 regulates oncogenesis through Akt and PI3K-dependent cytoskeletal remodeling...
  64. pmc Elongation factor 1a mediates the specificity of mitochondrial tRNA import in T. brucei
    Nabile Bouzaidi-Tiali
    Department of Biology Cell and Developmental Biology, University of Fribourg, Chemin du Musee 10, Fribourg, Switzerland
    EMBO J 26:4302-12. 2007
    ..This import requires eEF1a and aminoacylation of the tRNA. Thus, for a tRNA to be imported into the mitochondrion of T. brucei, it needs to bind eEF1a, and it is this interaction that mediates the import specificity...
  65. ncbi Immunofluorescence studies of human fibroblasts demonstrate the presence of the complex of elongation factor-1 beta gamma delta in the endoplasmic reticulum
    J Sanders
    Department of Medical Biochemistry, Sylvius Laboratory, University of Leiden, The Netherlands
    J Cell Sci 109:1113-7. 1996
    ..The guanine nucleotide-binding subunit of EF-1, EF-1 alpha, shows a more diffuse distribution throughout the cytoplasm and is, in addition, associated with the nucleus...
  66. ncbi eEF1A2 as a putative oncogene
    Mee Hyun Lee
    National Research Laboratory of Molecular Carcinogenesis and Chemoprevention, College of Pharmacy and Cancer Research Institute, Seoul National University, Seoul, Korea
    Ann N Y Acad Sci 1171:87-93. 2009
    ..This abnormal expression correlates with a poor prognosis. Since this discovery, there have been several reports suggesting eEF1A2 as a diagnostic marker in various cancers. This review highlights the oncogenic potential of eEF1A2...
  67. ncbi Mechanisms of the transfer of aminoacyl-tRNA from aminoacyl-tRNA synthetase to the elongation factor 1 alpha
    V S Reed
    Department of Chemistry, Georgetown University, Washington, D C 20057
    J Biol Chem 269:32932-6. 1994
    ..5 M NH4Cl, which enhances the binding of Asp-tRNA by EF1 alpha, hDRS-bound Asp-tRNA can be transferred directly to EF1 alpha. The implications of these results on the function of the multi-tRNA synthetase complex will be discussed...
  68. ncbi Expression of elongation factor-1 gamma-related sequence in human pancreatic cancer
    Y Lew
    Department of Medical Oncology, University of Texas M D Anderson Cancer Center, Houston 77030
    Pancreas 7:144-52. 1992
    ..The deduced amino acid sequence shared 53% identity with the amino acid sequence for the Artemia elongation factor-1 gamma...
  69. ncbi The human Y-encoded testis-specific protein interacts functionally with eukaryotic translation elongation factor eEF1A, a putative oncoprotein
    Tatsuo Kido
    Division of Cell and Developmental Genetics, Department of Medicine, Veterans Affairs Medical Center, University of California, San Francisco, CA 94121, USA
    Int J Cancer 123:1573-85. 2008
    ..Our results suggest that TSPY could exert its oncogenic function(s) by interacting with eEF1As and stimulating gene expression via its enhancements in protein synthesis and gene transcription...
  70. ncbi Application of a sensitive, specific and controlled real-time PCR assay to surveillance indicates a low prevalence of viral haemorrhagic septicaemia virus (VHSV) in wild herring, Clupea harengus L., in Scottish waters
    I Matejusova
    Marine Scotland, Marine Laboratory, Aberdeen, UK
    J Fish Dis 33:841-7. 2010
    ..The significance of migratory species such as herring as a reservoir species for VHSV, with the potential to translocate virus genotypes between geographical areas, is discussed...
  71. ncbi The human leucine zipper-containing guanine-nucleotide exchange protein elongation factor-1 delta
    J Sanders
    Department of Medical Biochemistry, Sylvius Laboratory, Leiden, The Netherlands
    Biochim Biophys Acta 1174:87-90. 1993
    ..The human EF-1 delta sequence shows a strong conservation in its C-terminal domain. The homology between the N-terminal domains of EF-1 delta proteins is low and almost exclusively limited to the leucine zipper motif...
  72. ncbi Deconstructing PTI-1: PTI-1 is a truncated, but not mutated, form of translation elongatin factor 1A1, eEF1A1
    Francisco Mansilla
    Department of Molecular Biology, University of Aarhus, Arhus C, Denmark
    Biochim Biophys Acta 1727:116-24. 2005
    ..However, we were unable to detect the PTI-1 protein in LNCaP cell extracts. The potential roles of the PTI-1 protein in carcinogenesis and the origin of the PTI-1 gene in the human genome are discussed...
  73. pmc BMP-6 promotes E-cadherin expression through repressing deltaEF1 in breast cancer cells
    Shuang Yang
    Medical College of Nankai University, Tianjin, China
    BMC Cancer 7:211. 2007
    ..Bone morphogenetic protein-6 (BMP-6) is critically involved in many developmental processes. Recent studies indicate that BMP-6 is closely related to tumor differentiation and metastasis...
  74. ncbi Phosphorylation of elongation factor 1 (EF-1) by protein kinase C stimulates GDP/GTP-exchange activity
    H I Peters
    Department of Biochemistry, University of California, Riverside 92521, USA
    Eur J Biochem 234:550-6. 1995
    ..Thus, the enhancement of EF-1 activity in response to 4 beta-phorbol 12-myristate 13-acetate appears to be due to stimulation of the rate of GDP/GTP exchange following phosphorylation of EF-1 by protein kinase C...
  75. pmc A structural domain mediates attachment of ethanolamine phosphoglycerol to eukaryotic elongation factor 1A in Trypanosoma brucei
    Eva Greganova
    Institute of Biochemistry and Molecular Medicine, University of Bern, Bern, Switzerland
    PLoS ONE 5:e9486. 2010
    ..Using T. brucei as a model organism, we show that amino acid substitutions around the modification site are not critical for EPG attachment and that a truncated version of domain III of eEF1A is sufficient to mediate EPG addition...
  76. ncbi The nucleotide sequence of the gene coding for the elongation factor 1 alpha in Sulfolobus solfataricus. Homology of the product with related proteins
    P Arcari
    Dipartimento di Biochimica e Biotecnologie Mediche, Universita di Napoli Federico II, Italy
    Biochim Biophys Acta 1217:333-7. 1994
    ..Alignments of aEF-1 alpha with several EF-1 alpha/EF-Tu revealed that aEF-1 alpha is more similar to its eukaryotic than to its eubacterial counterparts...
  77. pmc Identification of the human prostatic carcinoma oncogene PTI-1 by rapid expression cloning and differential RNA display
    R Shen
    Department of Pathology, Columbia University, College of Physicians and Surgeons, New York, NY 10032, USA
    Proc Natl Acad Sci U S A 92:6778-82. 1995
    ..The approaches used, rapid expression cloning with the CREF-Trans 6 system and the DD strategy, should prove widely applicable for identifying and cloning additional human oncogenes...
  78. ncbi Effect of elongation factor 1alpha promoter and SUMF1 over in vitro expression of N-acetylgalactosamine-6-sulfate sulfatase
    Carlos J Alméciga-Díaz
    Instituto de Errores Innatos del Metabolismo, Pontificia Universidad Javeriana, Bogota, D C, Colombia
    Mol Biol Rep 36:1863-70. 2009
    ..These results show the advantage of the SUMF1 coexpression on GALNS activity and indicate a considerable effect on the expression stability using EF1alpha promoter compared to CMV...
  79. pmc The primary structure of elongation factor EF-1 alpha from the brine shrimp Artemia
    F J Van Hemert
    EMBO J 3:1109-13. 1984
    ..A number of regions present in both EF-Tu and EF-G from Escherichia coli are also found in EF-1 alpha from Artemia...
  80. ncbi Elongation factor 1beta is an actin-binding protein
    R Furukawa
    Department of Cellular Biology, University of Georgia, 724 Biological Science Building, Athens, GA 30602, USA
    Biochim Biophys Acta 1527:130-40. 2001
    ....
  81. ncbi Interaction of G-rich GT oligonucleotides with nuclear-associated eEF1A is correlated with their antiproliferative effect in haematopoietic human cancer cell lines
    Bruna Scaggiante
    Department of Biochemistry, Biophysics and Macromolecular Chemistry, University of Trieste, Italy
    FEBS J 273:1350-61. 2006
    ..The cytotoxicity of G-rich GT oligomers was shown to be tightly related to their binding affinity for eEF1A protein...
  82. ncbi Site-directed mutants of post-translationally modified sites of yeast eEF1A using a shuttle vector containing a chromogenic switch
    J Cavallius
    Department of Biochemistry, School of Medicine, Case Western Reserve University, Cleveland, OH 44106 4935, USA
    Biochim Biophys Acta 1350:345-58. 1997
    ..There is also an apparent equivalency of wild-type and mutant yeast eEF1A in in vitro assays. It is concluded that the post-translational modifications of eEF1A are not of major importance for eEF1A's role in translation...
  83. ncbi Translation elongation factor 1A is essential for regulation of the actin cytoskeleton and cell morphology
    Stephane R Gross
    Department of Molecular Genetics, Microbiology and Immunology, University of Medicine and Dentistry of New Jersey Robert Wood Johnson Medical School, Piscataway, New Jersey 08854, USA
    Nat Struct Mol Biol 12:772-8. 2005
    ..This demonstrates for the first time a direct consequence of eEF1A on cytoskeletal organization in vivo and the physiological significance of this interaction...
  84. ncbi A structural model for elongation factor 1 (EF-1) and phosphorylation by protein kinase CKII
    G T Sheu
    Department of Biochemistry and Genetics Graduate Group, University of California, Riverside 92521, USA
    Mol Cell Biochem 191:181-6. 1999
    ....
  85. ncbi Candidate reference genes for gene expression studies in water lily
    Huolin Luo
    Nanjing Agricultural University, Jiangsu, People s Republic of China
    Anal Biochem 404:100-2. 2010
    ..ACT11 and AP47 also stably expressed in roots subjected to various treatments, but in the leaves of the same plants the most stably expressed genes were ubiquitin-conjugating enzyme 16 (UBC16) and ACT11...
  86. ncbi Validation of reference genes for real-time polymerase chain reaction studies in Atlantic salmon
    Sven Martin Jorgensen
    Department of Pharmaceutical Biosciences, School of Pharmacy, University of Oslo, 0316, Oslo, Norway
    Mar Biotechnol (NY) 8:398-408. 2006
    ..Software predictions supported a proper normalization is obtained combining 18S, EF1A, and RPL1 in vivo, but for in vitro viral infection assays we recommend using 18S...
  87. ncbi Protein elongation factor EEF1A2 is a putative oncogene in ovarian cancer
    Nisha Anand
    Hamilton Regional Cancer Centre, Room 450, 699 Concession Street, Hamilton, Ontario, L8V 5C2, Canada
    Nat Genet 31:301-5. 2002
    ..Thus, EEF1A2 and the process of protein elongation are likely to be critical in the development of ovarian cancer...
  88. pmc Translation elongation factor 1A is a component of the tombusvirus replicase complex and affects the stability of the p33 replication co-factor
    Zhenghe Li
    Department of Plant Pathology, University of Kentucky, Lexington, 40546, USA
    Virology 385:245-60. 2009
    ..We also demonstrate binding of eEF1A to the p33 replication protein and a known cis-acting element at the 3' end of TBSV RNA. Using a functional mutant of eEF1A, we provide evidence on the involvement of eEF1A in TBSV replication...
  89. pmc Proteasome-mediated degradation of cotranslationally damaged proteins involves translation elongation factor 1A
    Show Mei Chuang
    Department of Biochemistry, Robert Wood Johnson Medical School, 683 Hoes Lane, Piscataway, NJ 08854, USA
    Mol Cell Biol 25:403-13. 2005
    ..Our findings provide a mechanistic foundation for defining how cellular proteins are degraded cotranslationally...
  90. pmc Complex distribution of EFL and EF-1alpha proteins in the green algal lineage
    Geoffrey P Noble
    Canadian Institute for Advanced Research, Department of Botany, University of British Columbia, Vancouver, BC, Canada
    BMC Evol Biol 7:82. 2007
    ..This distribution singles out green algae as a particularly promising group to understand the origin of EFL and the effects of its presence on EF-1alpha...
  91. ncbi Interaction between the keratin cytoskeleton and eEF1Bgamma affects protein synthesis in epithelial cells
    Seyun Kim
    Department of Biological Chemistry, The Johns Hopkins University School of Medicine, 725 N Wolfe Street, Baltimore, Maryland 21205, USA
    Nat Struct Mol Biol 14:982-3. 2007
    ..These findings extend the emerging relationship between keratin proteins and the translational machinery...
  92. ncbi Interaction of the eukaryotic elongation factor 1A with newly synthesized polypeptides
    Yuka Hotokezaka
    Cellular Biochemistry and Biophysics Program, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    J Biol Chem 277:18545-51. 2002
    ..This association was demonstrated both by photo-cross-linking and by a functional refolding assay...
  93. pmc Proteins induced by telomere dysfunction and DNA damage represent biomarkers of human aging and disease
    Hong Jiang
    Institute of Molecular Medicine and Max Planck Research Group on Stem Cell Aging and Department of Internal Medicine III, University of Ulm, 89081 Ulm, Germany
    Proc Natl Acad Sci U S A 105:11299-304. 2008
    ..These results support the concept that telomere dysfunction and DNA damage are interconnected pathways that are activated during human aging and disease...
  94. ncbi Assignment of human elongation factor 1alpha genes: EEF1A maps to chromosome 6q14 and EEF1A2 to 20q13.3
    A Lund
    Department of Biostructural Chemistry, Aarhus University, Aarhus, Denmark
    Genomics 36:359-61. 1996
    ..3 by FISH. In addition, the 11 strongest cross-hybridizing loci (EEF1AL2-EEF1AL13) were mapped by FISH to 12p12, 9q34, 7p15-p21, 19q13, 3q26-q27, 7q33-q35, 1p13-p22, 2q12-q14, 5p12-q11, 1q31-q32, and Xq21...
  95. pmc Eukaryotic elongation factor 1A interacts with the upstream pseudoknot domain in the 3' untranslated region of tobacco mosaic virus RNA
    Vladimir V Zeenko
    Friedrich Miescher Institute, CH 4002 Basel, Switzerland
    J Virol 76:5678-91. 2002
    ..Its possible role in the regulation of tobamovirus gene expression and replication is discussed...
  96. pmc Translation elongation factor 1-alpha interacts specifically with the human immunodeficiency virus type 1 Gag polyprotein
    A Cimarelli
    Departments of Microbiology, College of Physicians and Surgeons, Columbia University, New York, New York 10032, USA
    J Virol 73:5388-401. 1999
    ....
  97. ncbi Prevalence of interspecific hybrids amongst asexual fungal endophytes of grasses
    C D Moon
    Department of Plant Sciences, University of Oxford, South Parks Road, Oxford OX1 3RB, UK
    Mol Ecol 13:1455-67. 2004
    ..Therefore, the abundance of hybrid species among grass endophytes, and their prevalence in many host populations suggests a selective advantage of hybridization for the mutualistic endophytes...
  98. pmc Peptide elongation factor eEF1A-2/S1 expression in cultured differentiated myotubes and its protective effect against caspase-3-mediated apoptosis
    Louis Bruno Ruest
    Bloomfield Center for Research in Aging, Lady Davis Institute for Medical Research, Sir Mortimer B Davis Jewish General Hospital, The Department of Medicine, McGill University, Montreal, Quebec H3T 1E2, Canada
    J Biol Chem 277:5418-25. 2002
    ..These results demonstrate that the two sister genes, eEF1A-1/EF-1alpha and eEF1A-2/S1, regulate myotube survival with the former exerting prodeath activity and the latter a prosurvival effect...
  99. pmc Internet-accessible DNA sequence database for identifying fusaria from human and animal infections
    Kerry O'Donnell
    Bacterial Foodborne Pathogens and Mycology Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, United States Department of Agriculture, Peoria, IL 61604 3999, USA
    J Clin Microbiol 48:3708-18. 2010
    ....
  100. ncbi Phylogenetic utility of elongation factor-1 alpha in noctuoidea (Insecta: Lepidoptera): the limits of synonymous substitution
    A Mitchell
    Department of Entomology, University of Maryland, College Park 20742, USA
    Mol Biol Evol 14:381-90. 1997
    ..EF-1 alpha synonymous changes show promise for phylogeny reconstruction within Noctuidae and other groups of Tertiary age...
  101. ncbi Efficient gene transfer into mouse embryonic stem cells with adenovirus vectors
    Kenji Kawabata
    Laboratory of Gene Transfer and Regulation, National Institute of Biomedical Innovation, Osaka 567 0085, Japan
    Mol Ther 12:547-54. 2005
    ..These results suggest that Ad vectors can be used for basic research using ES cells and that they may be of great utility for therapeutic applications in gene-modified regenerative medicine based on ES cells...

Research Grants20

  1. GENE EXPRESSION OF NEGATIVE STRAND RNA VIRUSES
    Amiya Banerjee; Fiscal Year: 2009
    ..The knowledge gleaned from these studies will help provide valuable information with regard to the mechanism of gene expression of VSV and by extension to other NNS RNA viruses. ..
  2. GENE STRUCTURE AND EF-1A IN THE FUNGUS MUCOR
    PAUL SYPHERD; Fiscal Year: 1991
    ..g., spore germination, yeast-to-hypha conversions, and sporulation). We also propose to study the 5'flanking regions of each gene to assess the mechanisms controlling transcription...
  3. Spontaneous Lytic Replication of KSHV
    Jeffrey Vieira; Fiscal Year: 2008
    ..2. Analyze the X219 genome to identify genetic differences compared to the parental virus. 3. Determine the genotype of X219 responsible for the phenotype of spontaneous lytic replication. ..
  4. Molecular genetics of translational accuracy
    Philip Farabaugh; Fiscal Year: 2007
    ..This work will be done both in bacteria and in yeast. We will also study which trans-acting proteins interact with this accuracy mechanism by studying the accuracy effect of a class of candidate gene deletions in yeast. ..
  5. Arp2/3 mRNA localization and directional cell migration
    Gang Liu; Fiscal Year: 2008
    ..unreadable] [unreadable]..
  6. SIGNALING COMPLEXES AND THE 14-3-3 PROTEIN IN CANDIDA
    Joy Sturtevant; Fiscal Year: 2008
    ..Consequently, identification of inhibitors of the processes identified in this proposal may prove to be a line of antifungal drug discovery. [unreadable] [unreadable] [unreadable]..
  7. Development of selenomethionine resistant yeast
    TERRI KINZY; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  8. Vacuole expansion during flamentation in Candida
    Joy Sturtevant; Fiscal Year: 2006
    ..The information obtained in this study on the role of the fungal vacuole in pathogenesis may also aid in the design of new anti-fungal treatment strategies. ..
  9. Targeting of somatic hypermutation and gene conversion
    David Schatz; Fiscal Year: 2006
    ..The results of these experiments should set the stage for significant progress toward an understanding of the mechanism by which protein factors and DNA sequences collaborate to target SM and GC to Ig genes. ..
  10. Discovery of New Cellular Targets of Anthrax Toxin
    ALINE SCANDURRO; Fiscal Year: 2004
    ..This body of information will also serve as the foundation for new research areas to explore in future proposals. ..
  11. IMMUNOGLOBULIN AND T CELL RECEPTOR GENE ASSEMBLY
    David Schatz; Fiscal Year: 2006
    ..Overall, our experiments will address both the targeting and rejoining functions of the RAG proteins, with the long term goal of linking these fundamental activities to chromosomal translocations found in human malignancies. ..
  12. AUTOMATED DNA ANALYSIS SYSTEM
    TERRI KINZY; Fiscal Year: 2001
    ..Trained personnel of the core facility will run and maintain all equipment to ensure optimal quality and speed. These personnel will also provide ongoing technical support to all users of this equipment. ..
  13. Structure of Eukaryotic Translation Elongation Factor 1
    TERRI KINZY; Fiscal Year: 2004
    ..With S. cerevisiae, the structural information obtained on eEF1A can be utilized for molecular genetic analysis of the critical residues for function and regulation. ..
  14. Oral-Pharyngeal HHV-8 Infection
    Jeffrey Vieira; Fiscal Year: 2004
    ..abstract_text> ..
  15. Molecular genetics of translational accuracy
    PHILIP JAMES FARABAUGH; Fiscal Year: 2010
    ..The conclusion of our work is that wobble errors commonly occur, tllOugh they are not universal, and that other types of errors occur when the competing ..
  16. Transmission and Acquisition of KSHV
    Jeffrey Vieira; Fiscal Year: 2008
    ..2. To investigate the transmission of KSHV across the oral mucosa using in vitro organ culture models. 3. To determine the cells of oral mucosa organ cultures that are sites of primary KSHV infection. ..
  17. The Molecular Basis of Pseudoexfoliation Syndrome
    Pratap Challa; Fiscal Year: 2007
    ..The Ophthalmology department at Duke University is an ideal setting for training physician-scientists and has an established track record of strong support for promising clinician-scientists. ..
  18. Mechanism of ribosomal subunit joining in eukaryotes
    Tatyana Pestova; Fiscal Year: 2005
    ....
  19. Molecular Analysis of Scarless Fetal Wound Healing
    Sandeep Kathju; Fiscal Year: 2007
    ..With these complementary approaches we hope to thereby arrive at a broad understanding of the gene patterns controlling both fetal and adult wound healing, and identify gene products/pathways critical to both. ..