osteocalcin

Summary

Summary: Vitamin K-dependent calcium-binding protein synthesized by OSTEOBLASTS and found primarily in BONES. Serum osteocalcin measurements provide a noninvasive specific marker of bone metabolism. The protein contains three residues of the amino acid gamma-carboxyglutamic acid (Gla), which, in the presence of CALCIUM, promotes binding to HYDROXYAPATITE and subsequent accumulation in BONE MATRIX.

Top Publications

  1. ncbi Osf2/Cbfa1: a transcriptional activator of osteoblast differentiation
    P Ducy
    Department of Molecular Genetics, M D Anderson Cancer Center, University of Texas, Houston 77030, USA
    Cell 89:747-54. 1997
  2. pmc Osteocalcin differentially regulates beta cell and adipocyte gene expression and affects the development of metabolic diseases in wild-type mice
    Mathieu Ferron
    Departments of Genetics and Development and Pathology, College of Physicians and Surgeons, Columbia University, New York, NY 10032, USA
    Proc Natl Acad Sci U S A 105:5266-70. 2008
  3. pmc Insulin signaling in osteoblasts integrates bone remodeling and energy metabolism
    Mathieu Ferron
    Department of Genetics and Development, College of Physicians and Surgeons, Columbia University, New York, NY 10032, USA
    Cell 142:296-308. 2010
  4. pmc A novel role for CCL3 (MIP-1α) in myeloma-induced bone disease via osteocalcin downregulation and inhibition of osteoblast function
    S Vallet
    Division of Hematology and Oncology, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA
    Leukemia 25:1174-81. 2011
  5. ncbi Serum undercarboxylated osteocalcin was inversely associated with plasma glucose level and fat mass in type 2 diabetes mellitus
    I Kanazawa
    Department of Internal Medicine 1, Faculty of Medicine, Shimane University, 89 1 Enya cho, Izumo, Shimane, 693 8501, Japan
    Osteoporos Int 22:187-94. 2011
  6. ncbi Plasma osteocalcin is inversely related to fat mass and plasma glucose in elderly Swedish men
    Jenny M Kindblom
    Center for Bone Research at the Sahlgrenska Academy, Institute of Medicine, The Sahlgrenska Academy at Goteborg University, Goteborg, Sweden
    J Bone Miner Res 24:785-91. 2009
  7. pmc Vitamin K-dependent carboxylation of osteocalcin: friend or foe?
    Caren M Gundberg
    Orthopaedics and Rehabilitation, Yale School of Medicine, New Haven, CT, USA
    Adv Nutr 3:149-57. 2012
  8. ncbi Bone morphogenetic proteins, extracellular matrix, and mitogen-activated protein kinase signaling pathways are required for osteoblast-specific gene expression and differentiation in MC3T3-E1 cells
    Guozhi Xiao
    Department of Periodontics, Prevention, and Geriatrics, School of Dentistry, University of Michigan, Ann Arbor 48109 1078, USA
    J Bone Miner Res 17:101-10. 2002
  9. ncbi The osteoblast: an insulin target cell controlling glucose homeostasis
    Thomas L Clemens
    Department of Orthopaedic Surgery, The Johns Hopkins University, Baltimore, MD 21287, USA
    J Bone Miner Res 26:677-80. 2011
  10. pmc TGF-beta-induced repression of CBFA1 by Smad3 decreases cbfa1 and osteocalcin expression and inhibits osteoblast differentiation
    T Alliston
    Department of Growth and Development, Program in Cell Biology, University of California at San Francisco, San Francisco, CA 94143-0640, USA
    EMBO J 20:2254-72. 2001

Detail Information

Publications349 found, 100 shown here

  1. ncbi Osf2/Cbfa1: a transcriptional activator of osteoblast differentiation
    P Ducy
    Department of Molecular Genetics, M D Anderson Cancer Center, University of Texas, Houston 77030, USA
    Cell 89:747-54. 1997
    ..We previously identified an osteoblast-specific cis-acting element, termed OSE2, in the Osteocalcin promoter. We have now cloned the cDNA encoding Osf2/Cbfa1, the protein that binds to OSE2...
  2. pmc Osteocalcin differentially regulates beta cell and adipocyte gene expression and affects the development of metabolic diseases in wild-type mice
    Mathieu Ferron
    Departments of Genetics and Development and Pathology, College of Physicians and Surgeons, Columbia University, New York, NY 10032, USA
    Proc Natl Acad Sci U S A 105:5266-70. 2008
    The osteoblast-specific secreted molecule osteocalcin behaves as a hormone regulating glucose metabolism and fat mass in two mutant mouse strains...
  3. pmc Insulin signaling in osteoblasts integrates bone remodeling and energy metabolism
    Mathieu Ferron
    Department of Genetics and Development, College of Physicians and Surgeons, Columbia University, New York, NY 10032, USA
    Cell 142:296-308. 2010
    ..expressing this receptor is the osteoblast, a bone-specific cell favoring glucose metabolism through a hormone, osteocalcin, that becomes active once uncarboxylated...
  4. pmc A novel role for CCL3 (MIP-1α) in myeloma-induced bone disease via osteocalcin downregulation and inhibition of osteoblast function
    S Vallet
    Division of Hematology and Oncology, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA
    Leukemia 25:1174-81. 2011
    ..MM-derived and exogenous CCL3 represses mineralization and osteocalcin production by primary human bone marrow stromal cells and HS27A cells...
  5. ncbi Serum undercarboxylated osteocalcin was inversely associated with plasma glucose level and fat mass in type 2 diabetes mellitus
    I Kanazawa
    Department of Internal Medicine 1, Faculty of Medicine, Shimane University, 89 1 Enya cho, Izumo, Shimane, 693 8501, Japan
    Osteoporos Int 22:187-94. 2011
    Although recent animal studies have shown that undercarboxylated osteocalcin acts as a hormone regulating glucose metabolism and fat mass, little is known about the relationships in humans...
  6. ncbi Plasma osteocalcin is inversely related to fat mass and plasma glucose in elderly Swedish men
    Jenny M Kindblom
    Center for Bone Research at the Sahlgrenska Academy, Institute of Medicine, The Sahlgrenska Academy at Goteborg University, Goteborg, Sweden
    J Bone Miner Res 24:785-91. 2009
    The osteoblast-derived protein osteocalcin has recently been shown to affect adiposity and glucose homeostasis in mice, suggesting that the skeleton influences energy metabolism through an endocrine mechanism...
  7. pmc Vitamin K-dependent carboxylation of osteocalcin: friend or foe?
    Caren M Gundberg
    Orthopaedics and Rehabilitation, Yale School of Medicine, New Haven, CT, USA
    Adv Nutr 3:149-57. 2012
    b>Osteocalcin originates from osteoblastic synthesis and is deposited into bone or released into circulation, where it correlates with histological measures of bone formation...
  8. ncbi Bone morphogenetic proteins, extracellular matrix, and mitogen-activated protein kinase signaling pathways are required for osteoblast-specific gene expression and differentiation in MC3T3-E1 cells
    Guozhi Xiao
    Department of Periodontics, Prevention, and Geriatrics, School of Dentistry, University of Michigan, Ann Arbor 48109 1078, USA
    J Bone Miner Res 17:101-10. 2002
    ..The ability of exogenously added BMP-2, BMP-4, or BMP-7 to stimulate osteocalcin (OCN) and bone sialoprotein (BSP) mRNAs or OCN promoter activity was synergistically increased in cells that ..
  9. ncbi The osteoblast: an insulin target cell controlling glucose homeostasis
    Thomas L Clemens
    Department of Orthopaedic Surgery, The Johns Hopkins University, Baltimore, MD 21287, USA
    J Bone Miner Res 26:677-80. 2011
    ..tasks is the role of bone in the control of energy metabolism, which is achieved through the secretion of osteocalcin, an osteoblasts-derived hormone regulating insulin secretion, insulin sensitivity, and energy expenditure...
  10. pmc TGF-beta-induced repression of CBFA1 by Smad3 decreases cbfa1 and osteocalcin expression and inhibits osteoblast differentiation
    T Alliston
    Department of Growth and Development, Program in Cell Biology, University of California at San Francisco, San Francisco, CA 94143-0640, USA
    EMBO J 20:2254-72. 2001
    ..TGF-beta inhibited the expression of the cbfa1 and osteocalcin genes, whose expression is controlled by CBFA1 in osteoblast-like cell lines...
  11. pmc Association of osteocalcin with obesity, insulin resistance, and cardiovascular risk factors in young adults
    Lynda E Polgreen
    Department of Pediatrics, University of Minnesota, Minneapolis, Minnesota, USA
    Obesity (Silver Spring) 20:2194-201. 2012
    Low levels of osteocalcin (OCN), an osteoblast-specific hormone, have recently been associated with insulin resistance (homeostasis model assessment-insulin resistance (HOMA-IR)) and obesity, particularly in older adults...
  12. ncbi Serum levels of osteocalcin are inversely associated with the metabolic syndrome and the severity of coronary artery disease in Chinese men
    Yuqian Bao
    Department of Endocrinology and Metabolism, Shanghai Diabetes Institute, Shanghai Key Laboratory of Diabetes Mellitus, Shanghai Clinical Center for Diabetes, Shanghai Jiao Tong University Affiliated Sixth People s Hospital, Shanghai, China
    Clin Endocrinol (Oxf) 75:196-201. 2011
    b>Osteocalcin is a bone-derived protein and has been shown to play an important role in regulating glucose and fat metabolism...
  13. ncbi Total and undercarboxylated osteocalcin predict changes in insulin sensitivity and β cell function in elderly men at high cardiovascular risk
    Monica Bullo
    Human Nutrition Unit, Facultat de Medicina i Ciencies de la Salut, IISPV, Universitat Rovira i Virgili, Reus, Spain
    Am J Clin Nutr 95:249-55. 2012
    b>Osteocalcin has been related to insulin secretion in experimental models. Few prospective studies have evaluated the association between circulating osteocalcin concentrations and insulin secretion and sensitivity in humans.
  14. ncbi Low serum osteocalcin level is a potential marker for metabolic syndrome: results from a Chinese male population survey
    Aihua Tan
    Center for Metabolic Disease and Diabetes, First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi Zhuang Autonomous Region, China
    Metabolism 60:1186-92. 2011
    b>Osteocalcin has been recognized as a bone-derived hormone to regulate energy metabolism recently. Little is known about the role of osteocalcin as regards metabolic syndrome (MetS) in a Chinese population...
  15. ncbi The role of osteocalcin in the endocrine cross-talk between bone remodelling and energy metabolism
    P Ducy
    Department of Pathology and Cell Biology, Columbia University Medical Center, New York, NY 10032, USA
    Diabetologia 54:1291-7. 2011
    ..Studies to test this hypothesis identified osteocalcin, a hormone secreted by osteoblasts, as a positive regulator of insulin secretion, insulin resistance and energy ..
  16. ncbi The relationship of serum osteocalcin concentration to insulin secretion, sensitivity, and disposal with hypocaloric diet and resistance training
    Jose Manuel Fernandez-Real
    Department of Diabetes, Institut d Investigació Biomédica de Girona, CIBER Fisiopatología de la Obesidad y Nutrición CB06 03 010, 17007 Girona, Catalonia, Spain
    J Clin Endocrinol Metab 94:237-45. 2009
    Bone has recently been described as exhibiting properties of an endocrine organ by producing osteocalcin that increases insulin sensitivity and secretion in animal models.
  17. ncbi Effects of genistein and hormone-replacement therapy on bone loss in early postmenopausal women: a randomized double-blind placebo-controlled study
    Nunziata Morabito
    Department of Internal Medicine, University of Messina, Italy
    J Bone Miner Res 17:1904-12. 2002
    ..Placebo administration did not change the serum levels of the bone-specific ALP (B-ALP) and osteocalcin (bone Gla protein [BGP])...
  18. ncbi Directing the expression of a green fluorescent protein transgene in differentiated osteoblasts: comparison between rat type I collagen and rat osteocalcin promoters
    Z Kalajzic
    Department of Genetics and Developmental Biology, University of Connecticut Health Center, Farmington, Connecticut 06030, USA
    Bone 31:654-60. 2002
    The osteocalcin (OC) and a 2.3 kb fragment of the collagen promoter (Col2.3) have been used to restrict transgenic expression of a variety of proteins to bone...
  19. ncbi Serum undercarboxylated osteocalcin levels are inversely associated with glycemic status and insulin resistance in an elderly Japanese male population: Fujiwara-kyo Osteoporosis Risk in Men (FORMEN) Study
    M Iki
    Department of Public Health, Kinki University Faculty of Medicine, 377 2 Oono Higashi, Osaka Sayama, Osaka 589 8511, Japan
    Osteoporos Int 23:761-70. 2012
    Recent animal studies have demonstrated that undercarboxylated osteocalcin upregulates insulin secretion via osteoblast-insulin signaling. However, it remains unclear whether such a pathway exists in humans...
  20. pmc BMP2 protein regulates osteocalcin expression via Runx2-mediated Atf6 gene transcription
    Won Gu Jang
    Dental Science Research Institute and BK21, School of Dentistry, Chonnam National University, Gwangju 500 757, Republic of Korea
    J Biol Chem 287:905-15. 2012
    ..Overall, these results suggest that BMP2 induces osteoblast differentiation through Runx2-dependent ATF6 expression, which directly regulates Oc transcription...
  21. pmc Change in undercarboxylated osteocalcin is associated with changes in body weight, fat mass, and adiponectin: parathyroid hormone (1-84) or alendronate therapy in postmenopausal women with osteoporosis (the PaTH study)
    Anne L Schafer
    Department of Medicine, University of California, San Francisco, San Francisco, California 94143, USA
    J Clin Endocrinol Metab 96:E1982-9. 2011
    The undercarboxylated form of the osteoblast-secreted protein osteocalcin has favorable effects on fat and glucose metabolism in mice. In human subjects, cross-sectional studies suggest a relevant association.
  22. ncbi Associations of total osteocalcin with all-cause and cardiovascular mortality in older men. The Health In Men Study
    B B Yeap
    School of Medicine and Pharmacology, University of Western Australia, Perth, WA, Australia
    Osteoporos Int 23:599-606. 2012
    In older men, both lower and higher total osteocalcin levels predict increased all-cause mortality, with comparable associations for cardiovascular and non-cardiovascular deaths...
  23. pmc Intermittent injections of osteocalcin improve glucose metabolism and prevent type 2 diabetes in mice
    Mathieu Ferron
    Department of Genetics and Development, College of Physicians and Surgeons, Columbia University, New York, NY 10032, USA
    Bone 50:568-75. 2012
    The uncarboxylated form of the osteoblast-specific secreted molecule osteocalcin is a hormone favoring glucose handling and increasing energy expenditure...
  24. ncbi Activation of peroxisome proliferator-activated receptor-gamma inhibits the Runx2-mediated transcription of osteocalcin in osteoblasts
    Min Jae Jeon
    Department of Internal Medicine, Seoul National University College of Medicine, Seoul 110 744, Korea
    J Biol Chem 278:23270-7. 2003
    ..that the activation of PPARgamma in osteoblasts suppresses osteoblast differentiation and the expression of osteocalcin, an osteoblast-specific protein. However, the mechanism of this inhibition remains unclear...
  25. ncbi Serum osteocalcin level is associated with glucose metabolism and atherosclerosis parameters in type 2 diabetes mellitus
    Ippei Kanazawa
    Department of Internal Medicine 1, Shimane University School of Medicine, 89 1 Enya cho, Izumo, Shimane 693 8501, Japan
    J Clin Endocrinol Metab 94:45-9. 2009
    Recent animal studies showed that osteocalcin action is related to not only bone metabolism but also glucose metabolism and fat mass...
  26. pmc Bone and glucose metabolism: a two-way street
    Katherine J Motyl
    Department of Physiology, Michigan State University, East Lansing, MI 48824, USA
    Arch Biochem Biophys 503:2-10. 2010
    Evidence from rodent models indicates that undercarboxylated osteocalcin (ucOC), a product of osteoblasts, is a hormone affecting insulin production by the pancreas and insulin sensitivity in peripheral tissues, at least in part through ..
  27. ncbi Fibroblast growth factor 2 induction of the osteocalcin gene requires MAPK activity and phosphorylation of the osteoblast transcription factor, Cbfa1/Runx2
    Guozhi Xiao
    Department of Periodontics, School of Dentistry, University of Michigan, Ann Arbor, Michigan 48109 1078, USA
    J Biol Chem 277:36181-7. 2002
    ..Biol. Chem. 275, 4453-4459). The present study examined the mechanism of FGF-2 regulation of the mouse osteocalcin gene in MC3T3-E1 preosteoblastic cells...
  28. ncbi Enhanced osteocalcin expression by osteoblast-like cells (MC3T3-E1) exposed to bioactive coating glass (SiO2-CaO-P2O5-MgO-K2O-Na2O system) ions
    V G Varanasi
    Division of Biomaterials and Bioengineering, University of California, San Francisco, CA 94143 0758, USA
    Acta Biomater 5:3536-47. 2009
    ..type 1 synthesis and in turn enhanced the expression of downstream markers alkaline phosphatase, Runx2 and osteocalcin during osteoblast differentiation...
  29. pmc Gamma-carboxylation of osteocalcin and insulin resistance in older men and women
    M Kyla Shea
    USDA Human Nutrition Research Center on Aging at Tufts University, Boston, MA 02111, USA
    Am J Clin Nutr 90:1230-5. 2009
    The skeletal protein osteocalcin is gamma-carboxylated by vitamin K. High serum uncarboxylated osteocalcin reflects low vitamin K status...
  30. ncbi Biphasic effects of TGFβ1 on BMP9-induced osteogenic differentiation of mesenchymal stem cells
    Rui Dong Li
    Department of Orthopaedic Surgery, The First Affiliated Hospital, Chongqing Medical University, Chongqing, China
    BMB Rep 45:509-14. 2012
    ..BMP9 in combination with low dose of TGFβ1 potentiates the expression of later osteogenic markers osteopontin, osteocalcin and collagen type 1 (COL1a2), while higher concentrations of TGFβ1 decrease the expression of osteopontin and ..
  31. pmc Distinct patterns of notochord mineralization in zebrafish coincide with the localization of Osteocalcin isoform 1 during early vertebral centra formation
    Anabela Bensimon-Brito
    PhD Program in Biomedical Sciences, University of Algarve, Faro, Portugal
    BMC Dev Biol 12:28. 2012
    ..This study compares initial patterns of mineralization in different regions of the vertebral column in zebrafish. We ask if the absence or presence of cartilaginous arches influences the pattern of notochord sheath mineralization...
  32. ncbi The role of simvastatin in the osteogenesis of injectable tissue-engineered bone based on human adipose-derived stromal cells and platelet-rich plasma
    Yongsheng Zhou
    Department of Prosthodontics, Peking University School and Hospital of Stomatology, Beijing 100081, PR China
    Biomaterials 31:5325-35. 2010
    ..phosphatase activity, more mineralization deposition and more expression of osteoblast-related genes such as osteocalcin, core binding factor alpha1, bone morphogenetic protein-2, vascular endothelial growth factor, and basic ..
  33. ncbi The effect of Pb(2+) on the structure and hydroxyapatite binding properties of osteocalcin
    T L Dowd
    Department of Pediatrics, Montefiore Medical Center, Albert Einstein College of Medicine, Bronx, NY 10467, USA
    Biochim Biophys Acta 1535:153-63. 2001
    ..binding assay to investigate the effect of Pb(2+) on the structure and mineral binding properties of osteocalcin, a noncollagenous bone protein...
  34. ncbi Inverse relationship between serum osteocalcin levels and visceral fat area in Chinese men
    Yuqian Bao
    Department of Endocrinology and Metabolism, Shanghai Jiao Tong University Affiliated Sixth People s Hospital, 600 Yishan Road, Shanghai 200233, China
    J Clin Endocrinol Metab 98:345-51. 2013
    The relationship between serum osteocalcin levels and body fat distribution remains unclear. This study aimed to investigate the association between serum osteocalcin levels and visceral fat area (VFA) in Chinese men.
  35. ncbi Osteocalcin, deoxypyridinoline and interleukin-1beta in peri-implant crevicular fluid of patients with peri-implantitis
    Masashi Murata
    Department of Oral Biological Science, Niigata University Graduate School of Medical and Dental Services, Niigata, Japan
    Clin Oral Implants Res 13:637-43. 2002
    The aim of the present study was to analyze the levels of osteocalcin, deoxypyridinoline (Dpd) and interleukin-1beta as markers of bone metabolism in peri-implant crevicular fluid (PICF) from peri-implantitis patients...
  36. ncbi Human osteocalcin and bone sialoprotein mediating osteomimicry of prostate cancer cells: role of cAMP-dependent protein kinase A signaling pathway
    Wen Chin Huang
    Molecular Urology and Therapeutics Program, Department of Urology and Winship Cancer Institute, Emory University School of Medicine, Atlanta, GA 30322, USA
    Cancer Res 65:2303-13. 2005
    b>Osteocalcin and bone sialoprotein are the most abundant noncollagenous bone matrix proteins expressed by osteoblasts...
  37. ncbi The endocrine role of the skeleton: background and clinical evidence
    Verena Schwetz
    Division of Endocrinology and Metabolism, Department of Internal Medicine, Medical University of Graz, Auenbruggerplatz 15, Graz, Austria
    Eur J Endocrinol 166:959-67. 2012
    ..The first pathway leads to a decrease in bioactivity of the osteoblast-produced hormone osteocalcin (OC) (part 2)...
  38. ncbi Serum concentrations of osteocalcin, procollagen type 1 N-terminal propeptide and beta-CrossLaps in obese subjects with varying degrees of glucose tolerance
    P Iglesias
    Department of Endocrinology, Hospital Universitario Ramon y Cajal, Madrid, Spain
    Clin Endocrinol (Oxf) 75:184-8. 2011
    To evaluate serum levels of osteocalcin (OC), procollagen type 1 N-terminal propeptide (P1PN) and beta-CrossLaps (beta-CTx) in obese subjects and their relationship with glucose metabolism parameters.
  39. ncbi Molecular modulation of osteocalcin and its relevance in diabetes (Review)
    José Rafael Villafán-Bernal
    Departamento de Biología Molecular y Genómica, Centro Universitario de Ciencias de Salud, Universidad de Guadalajara, Colonia Independencia, CP 44340 Guadalajara, Jalisco, Mexico
    Int J Mol Med 28:283-93. 2011
    b>Osteocalcin (OC) is encoded by the bone γ-carboxyglutamate (Gla) protein (BGLAP) gene, and it is released by osteoblasts during osteogenesis...
  40. ncbi Material-related effects of BMP-2 delivery systems on bone regeneration
    Antonio Hernandez
    Department of Chemical Engineering and Pharmaceutical Technology, Universidad de La Laguna, San Cristóbal de La Laguna, Spain
    Acta Biomater 8:781-91. 2012
    ..In contrast, the PLGA system resulted in complete regeneration with mature trabecular bone formation 8 weeks after implantation...
  41. ncbi Dietary ratio of n-6/n-3 PUFAs and docosahexaenoic acid: actions on bone mineral and serum biomarkers in ovariectomized rats
    Bruce A Watkins
    Center for Enhancing Foods to Protect Health, Lipid Chemistry and Molecular Biology Laboratory, Purdue University, West Lafayette, IN 47907 2009, USA
    J Nutr Biochem 17:282-9. 2006
    ..of the bone resorption biomarkers pyridinoline (Pyd) and deoxypyridinoline, whereas the bone formation marker osteocalcin was lowest in the HP groups...
  42. ncbi Biology without walls: the novel endocrinology of bone
    Gerard Karsenty
    Department of Genetics and Development, Columbia University, New York, NY 10032, USA
    Annu Rev Physiol 74:87-105. 2012
    ..Collectively, the body of work discussed below illustrates a new and unconventional role for bone in mammalian physiology...
  43. ncbi Age-related loss of muscle mass and bone strength in mice is associated with a decline in physical activity and serum leptin
    Mark W Hamrick
    Department of Cellular Biology and Anatomy, Medical College of Georgia, Augusta, GA 30912, USA
    Bone 39:845-53. 2006
    ..Our data show that mice lose a significant amount of muscle and bone tissue with age, and this loss of musculoskeletal tissue is accompanied by a drop in serum leptin and preceded by a significant decrease in physical activity...
  44. ncbi In vitro differentiation of embryonic stem cells into mineralized osteoblasts
    Nicole I zur Nieden
    Bayer AG, Research Toxicology, Wippertal, Germany
    Differentiation 71:18-27. 2003
    ..The major noncollagenous component of bone matrix, osteocalcin, was localized to the mineralized cells by immunofluorescence...
  45. pmc Osteocalcin positive CD133+/CD34-/KDR+ progenitor cells as an independent marker for unstable atherosclerosis
    Andreas J Flammer
    Division of Cardiovascular Diseases, Mayo Clinic and College of Medicine, 200 First Street SW, Rochester, MN 55905, USA
    Eur Heart J 33:2963-9. 2012
    ..In patients with coronary artery disease (CAD), a large fraction of EPCs carry the osteoblastic marker osteocalcin (OCN), which may mediate vascular calcification and abnormal repair...
  46. ncbi Different bone integration profiles of turned and acid-etched implants associated with modulated expression of extracellular matrix genes
    Takahiro Ogawa
    The Jane and Jerry Weintraub Center for Reconstructive Biotechnology, Division of Advanced Prosthodontics, Biomaterials and Hospital Dentistry, UCLA School of Dentistry, Los Angeles, California 90095 1668, USA
    Int J Oral Maxillofac Implants 18:200-10. 2003
    ..This study correlated morphologic profiles of bone-implant integration and extracellular matrix (ECM) gene expression in response to the placement of implants with different surface topographies...
  47. pmc Carboxylation of osteocalcin affects its association with metabolic parameters in healthy children
    Anna Prats-Puig
    Pediatrics, Dr Josep Trueta Hospital and Girona Institute for Biomedical Research, Girona, Spain
    Diabetes Care 33:661-3. 2010
    OBJECTIVE Osteocalcin (OC), a bone-derived protein, was recently shown to regulate metabolic pathways in mice. Undercarboxylated OC (ucOC), but not carboxylated OC (cOC), increases adiponectin and insulin secretion...
  48. pmc Biofunctionalization of a titanium surface with a nano-sawtooth structure regulates the behavior of rat bone marrow mesenchymal stem cells
    Wenjie Zhang
    Department of Prosthodontics, Ninth People s Hospital Affiliated to Shanghai Jiao Tong University, School of Medicine, Shanghai, China
    Int J Nanomedicine 7:4459-72. 2012
    ..A series of improved implant surfaces functionalized with nanoscale structures have been fabricated using various methods...
  49. pmc Increased bone formation and decreased osteocalcin expression induced by reduced Twist dosage in Saethre-Chotzen syndrome
    M Yousfi
    Laboratory on Osteoblast Biology and Pathology, , Affiliated Centre National de la Recherche Scientifique, , Paris, France
    J Clin Invest 107:1153-61. 2001
    ..In contrast, Twist mutant osteoblasts displayed a cell-autonomous reduction of osteocalcin mRNA expression in basal conditions and during osteogenesis...
  50. pmc Circulating osteocalcin level is associated with improved glucose tolerance, insulin secretion and sensitivity independent of the plasma adiponectin level
    Y C Hwang
    Division of Endocrinology and Metabolism, Department of Medicine, Kyung Hee University Hospital at Gangdong, Kyung Hee University School of Medicine, 149, Sangil dong, Kangdong Ku, Seoul 134 727, South Korea
    Osteoporos Int 23:1337-42. 2012
    In agreement with the results of animal studies, the plasma osteocalcin level is positively associated with improved glucose tolerance and insulin secretion and sensitivity...
  51. ncbi Is 1, 25-dihydroxyvitamin D3 an ideal substitute for dexamethasone for inducing osteogenic differentiation of human adipose tissue-derived stromal cells in vitro?
    Yong Sheng Zhou
    Department of Prosthodontics, School and Hospital of Stomatology, Peking University, Beijing 100081, China
    Chin Med J (Engl) 119:1278-86. 2006
    ....
  52. pmc Coronary endothelial dysfunction in humans is associated with coronary retention of osteogenic endothelial progenitor cells
    Mario Gossl
    Division of Cardiovascular Diseases, Mayo Clinic College of Medicine, Rochester, MN, USA
    Eur Heart J 31:2909-14. 2010
    ..We have recently identified EPC co-expressing the osteoblastic marker osteocalcin [OCN (+) EPC] and found that their numbers are increased in patients with early and late coronary ..
  53. pmc Osteoblasts mediate the adverse effects of glucocorticoids on fuel metabolism
    Tara C Brennan-Speranza
    Bone Research Program, ANZAC Research Institute, University of Sydney, Sydney, Australia
    J Clin Invest 122:4172-89. 2012
    ..Glucocorticoids also suppress osteoblast function, including osteocalcin synthesis...
  54. pmc Vimentin inhibits ATF4-mediated osteocalcin transcription and osteoblast differentiation
    Na Lian
    Department of Medicine, Vanderbilt University Center for Bone Biology, Vanderbilt University Medical Center, Nashville, Tennessee 37232, USA
    J Biol Chem 284:30518-25. 2009
    Activating transcription factor 4 (ATF4) is an osteoblast-enriched transcription factor that regulates osteocalcin transcription and osteoblast terminal differentiation. To identify functional partners of ATF4, we applied ROS17/2...
  55. ncbi Distinct stem cells subpopulations isolated from human adipose tissue exhibit different chondrogenic and osteogenic differentiation potential
    Tommaso Rada
    3B s Research Group Biomaterials, Biodegradables and Biomimetics, Department of Polymer Engineering, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, Avepark, 4806 909 Taipas, Guimaraes, Portugal
    Stem Cell Rev 7:64-76. 2011
    ..CD90) as well as known markers of the chondrogenic (Sox 9, Collagen II) and osteogenic lineage (Osteopontin, Osteocalcin)...
  56. pmc Foxo1 mediates insulin-like growth factor 1 (IGF1)/insulin regulation of osteocalcin expression by antagonizing Runx2 in osteoblasts
    Shengyong Yang
    Department of Medicine, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15240, USA
    J Biol Chem 286:19149-58. 2011
    ..of insulin-like growth factor 1 (IGF1)/insulin actions, regulates Runx2 activity and expression of the mouse osteocalcin gene 2 (Bglap2) in osteoblasts in vitro...
  57. ncbi The effect of soy protein isolate on bone metabolism
    J Christopher Gallagher
    Bone Metabolism Unit, School of Medicine and the Osteoporosis Research Center, Creighton University, Omaha, NE, USA
    Menopause 11:290-8. 2004
    ..This double-blind, 15-month pilot study was designed to investigate the effect of soy protein isolate with varying concentrations of isoflavones on early postmenopausal bone loss and lipids...
  58. ncbi The effects of enamel matrix derivative (EMD) on osteoblastic cells in culture and bone regeneration in a rat skull defect
    Satoshi Yoneda
    Masticatory Function Control, Tokyo Medical and Dental University, Yushima, Tokyo, Japan
    J Periodontal Res 38:333-42. 2003
    ..Enamel matrix derivative (EMD) has been clinically used to promote periodontal tissue regeneration. The purpose of the present study is to clarify EMD affects on osteoblastic cells and bone regeneration...
  59. ncbi Ghrelin regulates proliferation and differentiation of osteoblastic cells
    Giuseppina Maccarinelli
    Department of Biomedical Sciences and Biotechnology, University of Brescia, Brescia, Italy
    J Endocrinol 184:249-56. 2005
    ..Ghrelin and HEXA significantly increased alkaline phosphatase (ALP) and osteocalcin (OC) production. At variance with these peptides, EP-40737 did not significantly stimulate ALP and OC...
  60. ncbi Widespread increase in myeloid calcifying cells contributes to ectopic vascular calcification in type 2 diabetes
    Gian Paolo Fadini
    Department of Clinical and Experimental Medicine, Metabolic Division, University of Padova, Medical School, Padova, Italy
    Circ Res 108:1112-21. 2011
    ..Acquisition of a procalcific phenotype by resident or circulating cells is important for calcification of atherosclerotic plaques, which is common in diabetes...
  61. ncbi MAPK pathways activate and phosphorylate the osteoblast-specific transcription factor, Cbfa1
    G Xiao
    Department of Periodontics, Prevention, and Geriatrics, School of Dentistry, University of Michigan, Ann Arbor, Michigan 48109 1078, USA
    J Biol Chem 275:4453-9. 2000
    The bone-specific transcription factor, Cbfa1, regulates expression of the osteocalcin (OCN) gene and is essential for bone formation. However, little is known about the mechanisms regulating Cbfa1 activity...
  62. pmc Endocrine regulation of male fertility by the skeleton
    Franck Oury
    Department of Genetics and Development, Columbia University, New York, NY 10032, USA
    Cell 144:796-809. 2011
    ..Analyses of cell-specific loss- and gain-of-function models reveal that the osteoblast-derived hormone osteocalcin performs this endocrine function...
  63. ncbi Serum osteocalcin level is positively associated with insulin sensitivity and secretion in patients with type 2 diabetes
    Ippei Kanazawa
    Department of Internal Medicine 1, Shimane University School of Medicine, 89 1 Enya cho, Izumo, Shimane, 693 8501, Japan
    Bone 48:720-5. 2011
    Bone is being recognized as an endocrine organ. Although previous animal studies showed that osteocalcin stimulated the expression of insulin in islets and of adiponectin in adipocytes with increased insulin secretion and sensitivity, ..
  64. pmc FoxO1 expression in osteoblasts regulates glucose homeostasis through regulation of osteocalcin in mice
    Marie Therese Rached
    Department of Medicine, Division of Endocrinology, College of Physicians and Surgeons, Columbia University, New York, NY, USA
    J Clin Invest 120:357-68. 2010
    Osteoblasts have recently been found to play a role in regulating glucose metabolism through secretion of osteocalcin. It is unknown, however, how this osteoblast function is regulated transcriptionally...
  65. ncbi Ghrelin directly regulates bone formation
    Nobuhiro Fukushima
    Molecular Genetics, Institute of Life Science, Kurume University, Kurume, Fukuoka, Japan
    J Bone Miner Res 20:790-8. 2005
    ..Ghrelin and its receptor, GHS-R1a, were identified in osteoblasts, and ghrelin promoted both proliferation and differentiation. Furthermore, ghrelin increased BMD in rats. Our results show that ghrelin directly affects bone formation...
  66. pmc Serum osteocalcin is associated with measures of insulin resistance, adipokine levels, and the presence of metabolic syndrome
    Umer Saleem
    Division of Cardiovascular Diseases, Mayo Clinic, 200 First Street SW, Rochester, MN 55905, USA
    Arterioscler Thromb Vasc Biol 30:1474-8. 2010
    b>Osteocalcin has been reported to influence insulin secretion in experimental animals...
  67. ncbi The uncarboxylated form of osteocalcin is associated with improved glucose tolerance and enhanced beta-cell function in middle-aged male subjects
    You Cheol Hwang
    Division of Endocrinology and Metabolism, Department of Medicine, Kyung Hee East West Neo Medical Center, Kyung Hee University School of Medicine, Seoul, Republic of Korea
    Diabetes Metab Res Rev 25:768-72. 2009
    Recent human studies support the notion that serum osteocalcin increases beta-cell proliferation and insulin secretion, and improves insulin sensitivity...
  68. ncbi Fabrication of pillar-like titania nanostructures on titanium and their interactions with human skeletal stem cells
    Terje Sjostrom
    Department of Oral and Dental Science, University of Bristol, Lower Maudlin Street, Bristol BS1 2LY, UK
    Acta Biomater 5:1433-41. 2009
    ..The 15 nm high topography features resulted in the greatest cell response with bone matrix nodule forming on the Ti surface after 21 days...
  69. ncbi Bone as an endocrine organ
    Seiji Fukumoto
    Division of Nephrology and Endocrinology, Department of Medicine, University of Tokyo Hospital, 7 3 1, Hongo, Bunkyo ku, Tokyo 113 8655, Japan
    Trends Endocrinol Metab 20:230-6. 2009
    ..evidence shows that the skeleton itself produces at least two hormones, fibroblast growth factor 23 (FGF23) and osteocalcin. FGF23 is produced by osteocytes in bone and acts on the kidney to inhibit 1alpha-hydroxylation of vitamin D ..
  70. ncbi Evidence for osteocalcin production by adipose tissue and its role in human metabolism
    Carlo Foresta
    Department of Histology, Internal Medicine 3, University of Padova, Italy
    J Clin Endocrinol Metab 95:3502-6. 2010
    The adipose tissue (AT), which is an endocrine organ, is linked to several metabolic abnormalities. Undercarboxylated osteocalcin (ucOCN) regulates insulin and adiponectin secretion.
  71. ncbi TAZ, a transcriptional modulator of mesenchymal stem cell differentiation
    Jeong Ho Hong
    Center for Cancer Research, Department of Biology, Massachusetts Institute of Technology, 77 Massachusetts Avenue, E18 580, Cambridge, MA 02139, USA
    Science 309:1074-8. 2005
    ..These results indicate that TAZ functions as a molecular rheostat that modulates MSC differentiation...
  72. pmc Therapeutic potential of vasculogenesis and osteogenesis promoted by peripheral blood CD34-positive cells for functional bone healing
    Tomoyuki Matsumoto
    Stem Cell Translational Research, Kobe Institute of Biomedical Research and Innovation RIKEN Center for Developmental Biology, 2 2 Minatojima Minamimachi, Chuo Ku, Kobe 650 0047
    Am J Pathol 169:1440-57. 2006
    ..blood CD34+ cells, isolated from total mononuclear cells of adult human volunteers, showed gene expression of osteocalcin in 4 of 20 freshly isolated cells by single cell reverse transcriptase-polymerase chain reaction analysis...
  73. ncbi Serum osteocalcin concentrations in relation to glucose and lipid metabolism in Chinese individuals
    Mi Zhou
    Shanghai Key Laboratory of Diabetes Mellitus, Department of Endocrinology and Metabolism, Shanghai Diabetes Institute, Shanghai Clinical Center for Diabetes, Shanghai Jiao Tong University Affiliated Sixth People s Hospital, 600 Yishan Road, Shanghai 200233, People s Republic of China
    Eur J Endocrinol 161:723-9. 2009
    b>Osteocalcin, a bone-derived protein, has recently been reported to affect energy metabolism. We investigated the relationship between serum osteocalcin and parameters of adiposity, glucose tolerance, and lipid profile in Chinese subjects.
  74. ncbi Myeloma cells block RUNX2/CBFA1 activity in human bone marrow osteoblast progenitors and inhibit osteoblast formation and differentiation
    Nicola Giuliani
    Laboratory of Hematology, Hematology and Bone Marrow Transplantation BMT Center, Department of Internal Medicine and Biomedical Science, University of Parma, Parma, Italy
    Blood 106:2472-83. 2005
    ..Moreover, an inhibitory effect on osteocalcin, alkaline phosphatase, collagen I mRNA, protein expression, and RUNX2/CBFA1 activity by human preosteoblastic ..
  75. ncbi Vitamin K status and bone health: an analysis of methods for determination of undercarboxylated osteocalcin
    C M Gundberg
    Department of Orthopaedics and Rehabilitation, Yale University School of Medicine, New Haven, Connecticut 06510, USA
    J Clin Endocrinol Metab 83:3258-66. 1998
    Recent studies suggest that fracture risk is associated with increased undercarboxylated osteocalcin. Methods use differences in binding of undercarboxylated and fully carboxylated osteocalcin to hydroxyapatite or barium sulfate...
  76. ncbi Reduced serum total osteocalcin is associated with metabolic syndrome in older men via waist circumference, hyperglycemia, and triglyceride levels
    Bu B Yeap
    School of Medicine and Pharmacology, University of Western Australia, Perth, Western Australia 6009, Australia
    Eur J Endocrinol 163:265-72. 2010
    Bone-derived undercarboxylated osteocalcin regulates insulin secretion and sensitivity in mice, and reduced serum total osteocalcin (TOC) is associated with diabetes in humans...
  77. pmc Osteocalcin expression by circulating endothelial progenitor cells in patients with coronary atherosclerosis
    Mario Gossl
    Division of Cardiology, College of Medicine, Mayo Clinic, Rochester, Minnesota 55905, USA
    J Am Coll Cardiol 52:1314-25. 2008
    ..This study was designed to test whether patients with coronary atherosclerosis have increases in circulating endothelial progenitor cells (EPCs) expressing an osteogenic phenotype...
  78. ncbi Osteocalcin and matrix Gla protein in zebrafish (Danio rerio) and Senegal sole (Solea senegalensis): comparative gene and protein expression during larval development through adulthood
    Paulo J Gavaia
    CCMAR, Universidade do Algarve, 8005 139 Faro, Portugal
    Gene Expr Patterns 6:637-52. 2006
    Bone Gla protein (Bgp or osteocalcin) and matrix Gla protein (Mgp) are important in calcium metabolism and skeletal development, but their precise roles at the molecular level remain poorly understood...
  79. ncbi Relationship between osteocalcin and glucose metabolism in postmenopausal women
    Jee Aee Im
    Department of Laboratory Medicine, MizMedi Hospital, 701 4 Naebalsan dong, Gangseo Gu, and Department of Sport and Leisure Studies, Yonsei University, Seoul, South Korea
    Clin Chim Acta 396:66-9. 2008
    Recently, osteocalcin was found to regulate blood glucose, insulin secretion, and fat deposition in mice...
  80. ncbi Altered bone and mineral metabolism in patients receiving imatinib mesylate
    Ellin Berman
    Department of Medicine, Memorial Sloan Kettering Cancer Center, New York, NY 10021, USA
    N Engl J Med 354:2006-13. 2006
    ..We observed that hypophosphatemia developed in some patients with either chronic myelogenous leukemia or gastrointestinal stromal tumors who were receiving imatinib...
  81. ncbi The DASH diet and sodium reduction improve markers of bone turnover and calcium metabolism in adults
    Pao Hwa Lin
    Department of Medicine, Sarah W Stedman Center for Nutritional Studies, Duke University Medical Center, Durham, NC, USA
    J Nutr 133:3130-6. 2003
    ..Serum osteocalcin (OC), C-terminal telopeptide of type I collagen (CTX), fasting serum parathyroid hormone (PTH), urinary sodium, ..
  82. ncbi Ankylosing spondylitis, psoriatic arthritis, and reactive arthritis show increased bone resorption, but differ with regard to bone formation
    Johannes Grisar
    Department of Internal Medicine III, University of Vienna, Austria
    J Rheumatol 29:1430-6. 2002
    ..To test if markers of bone metabolism are altered in patients with seronegative spondyloarthropathies (SSpA)...
  83. ncbi Diagnostic performance of salivary cortisol and serum osteocalcin measurements in patients with overt and subclinical Cushing's syndrome
    Marta Sereg
    2nd Department of Medicine, Semmelweis University, Szentkiralyi u 46, Budapest H 1088, Hungary
    Steroids 76:38-42. 2011
    ..Because serum osteocalcin (OC) is considered as a sensitive marker of hypercortisolism, we also investigated whether OC could have a role ..
  84. pmc Stromal derived factor-1 regulates bone morphogenetic protein 2-induced osteogenic differentiation of primary mesenchymal stem cells
    Naobumi Hosogane
    Muskuloskeletal Integrity Program, Hospital for Special Surgery, New York, NY 10021, USA
    Int J Biochem Cell Biol 42:1132-41. 2010
    ..or adding SDF-1 protein to MSCs significantly affected BMP2-induced alkaline phosphatase (ALP) activity and osteocalcin (OCN) synthesis, markers of preosteoblasts and mature osteoblasts, respectively...
  85. pmc Osterix enhances BMSC-associated osseointegration of implants
    B Xu
    Division of Oral Biology, Tufts University School of Dental Medicine, One Kneeland Street, Boston, MA 02111, USA
    J Dent Res 88:1003-7. 2009
    ..We concluded that exogenous BMSCs participate in the osseointegration after implantation, and that Osx overexpression accelerates osseointegration...
  86. ncbi Osteocalcin secretion as an early marker of in vitro osteogenic differentiation of rat mesenchymal stem cells
    Akifumi Nakamura
    Department of Orthopedic Surgery, Health Management, and Policy, School of Medicine, Nara Medical University, Kashihara, Nara, Japan
    Tissue Eng Part C Methods 15:169-80. 2009
    b>Osteocalcin (OC) is a bone-specific protein synthesized by osteoblasts that represents a good marker for osteogenic maturation...
  87. pmc Co-dependence of bone and energy metabolisms
    Jianwen Wei
    Department of Genetics and Development, Columbia University, New York, NY 10032, USA
    Arch Biochem Biophys 503:35-40. 2010
    ..This review is intended to summarize the key events that led to the identification and the characterization of the different steps and molecules constituting this regulatory network...
  88. ncbi Structure, activity, and distribution of fish osteocalcin
    Satoru K Nishimoto
    Molecular Sciences Department, University of Tennessee, Health Science Center, Memphis, Tennessee 38163, USA
    J Biol Chem 278:11843-8. 2003
    b>Osteocalcin (bone Gla protein) is an extracellular matrix protein synthesized by osteoblasts that is a marker of bone...
  89. ncbi The suppressive effect of enamel matrix derivative on osteocalcin gene expression of osteoblasts is neutralized by an antibody against TGF-beta
    Yoshiyuki Wada
    Department of Oral Health Science, Graduate School of Dentistry, Hokkaido University, Sapporo, Japan
    J Periodontol 79:341-7. 2008
    ..However, the precise mechanism of tissue regeneration by EMD remains obscure. The purpose of this study was to clarify what factors present in EMD show bioactivity...
  90. pmc Relationship of testosterone and osteocalcin levels during growth
    Salman Kirmani
    College of Medicine, Mayo Clinic, Rochester, MN 55905, USA
    J Bone Miner Res 26:2212-6. 2011
    Recent studies in mice have demonstrated that osteocalcin (OCN) regulates testosterone (T) production in males but not in females...
  91. ncbi Lower osteocalcin and osteopontin contents of the femoral head in hip fracture patients than osteoarthritis patients
    S Tanaka
    Department of Orthopaedic Surgery, Saitama Medical Center, Saitama Medical University, 1981 Kamoda, Kawagoe, Saitama, 350 8550, Japan
    Osteoporos Int 22:587-97. 2011
    ..The osteocalcin/deoxypyridinoline ratio and osteopontin/calcium ratio of cortical bone were selected as significant predictors.
  92. ncbi Relationship between serum levels of osteocalcin and atherosclerotic disease in type 2 diabetes
    R Reyes-García
    Bone Metabolic Unit RETICEF, Endocrinology Division, Hospital Universitario San Cecilio, Av Dr Oloriz 16, 18012 Granada, Spain
    Diabetes Metab 38:76-81. 2012
    To analyze the relationship between serum levels of osteocalcin and parameters of atherosclerosis in patients with type 2 diabetes mellitus (T2DM).
  93. ncbi Osteocalcin: genetic and physical mapping of the human gene BGLAP and its potential role in postmenopausal osteoporosis
    M H Raymond
    Department of Preventive Medicine and Environmental Health, University of Iowa, Iowa City, Iowa 52242, USA
    Genomics 60:210-7. 1999
    b>Osteocalcin is an abundant, highly conserved bone-specific protein that is synthesized by osteoblasts...
  94. ncbi The relationship between adipokines, osteocalcin and bone quality in chronic kidney disease
    Justine Bacchetta
    Centre de Référence des Maladies Rénales Rares, Hopital Femme Mere Enfant, Bron, France
    Nephrol Dial Transplant 24:3120-5. 2009
    b>Osteocalcin, a small peptide secreted by osteoblasts, has been recently described as a circulating hormone involved in the regulation of energy metabolism...
  95. ncbi Performance evaluation of automated assays for beta-CrossLaps, N-MID-Osteocalcin and intact parathyroid hormone (BIOROSE Multicenter Study)
    Heinrich Schmidt-Gayk
    Department of Endocrinology and Oncology, Limbach Laboratory, Im Breitspiel 15, 69126 Heidelberg, Germany
    Clin Chem Lab Med 42:90-5. 2004
    ..This has been shown in several external quality assessment schemes (EQAS)...
  96. ncbi Circulating osteocalcin is increased in early-stage diabetes
    Atsushi Aoki
    First Department of Comprehensive Medicine, Saitama Medical Center, Jichi Medical University School of Medicine, 1 847 Amanuma, Omiya, Saitama 330 8503, Japan
    Diabetes Res Clin Pract 92:181-6. 2011
    We aimed to examine whether circulating levels of osteocalcin, bone formation marker secreted from osteoblast, are changed in glucose-intolerant subjects without taking glucose lowering agent, because bone metabolism is reportedly ..
  97. ncbi Necessity of enzymatic activity of alkaline phosphatase for mineralization of osteoblastic cells
    Yuki Sugawara
    Department of Oral Functional Science, Graduate School of Dental Medicine, Hokkaido University, Sapporo, Japan
    Jpn J Pharmacol 88:262-9. 2002
    ..tetramisole showed no effect on cell growth and increased hydroxyproline accumulation, it decreased the osteocalcin production and the accumulation of calcium and phosphate in the matrices...
  98. ncbi Osteocalcin as a predictor of the metabolic syndrome in older persons: a population-based study
    Mirjam M Oosterwerff
    Department of Internal Medicine, Section Endocrinology, VU University Medical Center, Amsterdam, The Netherlands
    Clin Endocrinol (Oxf) 78:242-7. 2013
    Recent evidence indicates that the osteoblast-derived protein osteocalcin is able to influence adiposity and glucose homeostasis in mice. Little is known about this relationship in humans.
  99. pmc Genetic evidence points to an osteocalcin-independent influence of osteoblasts on energy metabolism
    Yoshihiro Yoshikawa
    Department of Medicine, Division of Endocrinology, College of Physicians and Surgeons, Columbia University, New York, NY, USA
    J Bone Miner Res 26:2012-25. 2011
    The skeleton has been shown recently to regulate glucose metabolism through an osteoblast-specific hormone, osteocalcin, which favors β-cell proliferation, insulin secretion, insulin sensitivity, and energy expenditure...
  100. ncbi The effects of enamel matrix derivative on the proliferation and differentiation of human mesenchymal stem cells
    Seong Suk Jue
    Department of Oral Anatomy, School of Dentistry, Kyung Hee University, Seoul, Korea
    Clin Oral Implants Res 21:741-6. 2010
    ..This study was designed to investigate the effect of enamel derivative matrix (EMD) on the proliferation, mineralization, and differentiation of human mesenchymal stem cells (hMSCs)...
  101. ncbi Effects of enamel matrix derivative on gene expression of primary osteoblasts
    J Jiang
    Department of Endodontology, School of Dental Medicine, University of Connecticut Health Center, Farmington, USA
    Oral Surg Oral Med Oral Pathol Oral Radiol Endod 91:95-100. 2001
    ..was to investigate the effects of enamel matrix derivative (EMD) on gene expression of collagen alpha1 (I), osteocalcin, prostaglandin G/H synthase 2 (PGHS-2), interleukin-6, and insulin-like growth factor I in primary mouse ..

Research Grants63

  1. Osteocalcin and Metabolic Risk Factors. The Framingham Study
    Yi Hsiang Hsu; Fiscal Year: 2011
    DESCRIPTION (provided by applicant): Based on a recent discovery in rodents, for the first time, osteocalcin (OC), a major bone formation protein uniquely secreted by osteoblasts, may play a crucial role in endocrine regulation of ..
  2. Biology of IGFs in Bone
    Thomas L Clemens; Fiscal Year: 2013
    ..Second, insulin induces production of osteocalcin (OC), which influences glucose utilization and energy expenditure...
  3. BBP (Bone Morphogenetic Protein Binding Peptide) and Bone Healing
    SAMUEL SCOTT MURRAY; Fiscal Year: 2013
    ..bone formation and chondrogenesis by quantitative histology and immunohistology of types I and II collagen and osteocalcin. In Specific Aim 5, we will synthesize cBBP derivatives with decreased KDs (increased affinities) for BMPs or ..
  4. Undercarboxylated osteocalcin, body fat, and diabetes in older adults
    Ann V Schwartz; Fiscal Year: 2010
    ..A product of osteoblasts, undercarboxylated osteocalcin (ucOC), functions as a hormone regulating insulin sensitivity and production, an unexpected connection that ..
  5. Salivary-based Bone-loss Marker Detection Platform for Point-of-Care Screening
    MANAL BESHAY; Fiscal Year: 2012
    ..This platform will assess two key bone activities: bone formation, presented in osteocalcin (OC), and bone resorption, presented in collagen crosslink deoxypyridinoline (D-PYR)...
  6. Conditional Knockout of Calcium Receptors in Bone Cells
    Dolores M Shoback; Fiscal Year: 2013
    ..specific Tg mice expressing Cre recombinase under the control of specific bone promoters (type 1 collagen, osteocalcin, osterix, dentin matrix protein 1) to develop conditional knockouts of the CaR targeted to bone cell ..
  7. Regulation of glucose metabolism by bone resorption and gamma-carboxylation
    Mathieu Ferron; Fiscal Year: 2012
    ..We have identified osteocalcin a hormone produced by the osteoblasts, the bone forming cells, as a previously uncharacterized regulator of ..
  8. Effect of Perinatal Diet on Developmental Programming of the Skeleton
    Mary L Bouxsein; Fiscal Year: 2010
    ..strength by biomechanical testing;serum adipokines (leptin, adiponectin), IGF-1 and markers of bone turnover (osteocalcin, TRACP5b, sCTx) by ELISA;and static and dynamic indices of bone formation/resorption, marrow adiposity and ..
  9. Classical and non-classical responses to vitamin D in children: the role of DBP g
    Thomas O Carpenter; Fiscal Year: 2010
    ..and mineral system, with outcome measures to include: circulating levels of PTH, alkaline phosphatase activity, osteocalcin, P1NP, and FGF23 as markers of a vitamin D effect on the skeleton and bone mineral density by ultrasound;and b) ..
  10. Establishing the Precursors of Osteoporosis in Children
    Xiping Xu; Fiscal Year: 2010
    ..markers: osteoprotegerin (OPG), soluble receptor activator of NFKB ligand (sRANKL), parathyroid hormone (PTH), osteocalcin (OC), tartrate-resistant acid phosphatase 5b (TRAP-5b);nutritional markers: magnesium, vitamin K1, and 25(OH) ..
  11. Delayed pubertal development on the mechanism of bone loss at maturity
    Vanessa R Yingling; Fiscal Year: 2011
    ..biomarkers will be measured to assess the response of pubertal delay on 23 systemic changes in bone metabolism osteocalcin (a marker of bone formation) and N-telopeptide of collagen 24 type I (NTx) (a marker of bone resorption)...
  12. The role of IFT80 in bone formation
    Shuying Yang; Fiscal Year: 2013
    ..osteoblast differentiation through down-regulating the expression of osteoblast marker genes- Runx2 and osteocalcin, and hedgehog signaling (Hh) related genes -shh, Ihh and Gli2...
  13. Role of Gsalpha in Regulating Osteoblast Differentiation
    JOY Y WU; Fiscal Year: 2012
    ..Furthermore expression of osteocalcin, a marker of terminally differentiated osteoblasts, is almost absent...
  14. Effect of botulinum toxin plus vibration on bone in children with cerebral palsy
    Christopher M Modlesky; Fiscal Year: 2012
    ..will be assessed using magnetic resonance imaging, changes in bone formation will be assessed using serum osteocalcin, and changes in bone resorption will be assessed using C-terminal telopeptide of type I collagen (CTX-1)...
  15. Targeted delivery of osteoinductive molecules to biologic HA in bone grafts
    Jennifer L Bain; Fiscal Year: 2012
    ..Bone formation markers such as alkaline phosphatase and osteocalcin will be measured. In vivo we will evaluate the anchored grafts ability to form bone in a subcutaneous rat model...
  16. Discovery of an Osteocalcin Sensing GPCR Regulating Beta-Cell Function
    L Darryl Quarles; Fiscal Year: 2013
    ..also an endocrine organ that regulates energy metabolism through the release of the osteoblast-derived hormone, osteocalcin (Ocn)...
  17. Wear Particle Disease and NF-kappa B Signaling
    Stuart B Goodman; Fiscal Year: 2013
    ..OPC cultures will be assayed for alkaline phosphatase and osteocalcin expression, and bone matrix formation will be quantitated using Von Kossa staining...
  18. Osteocalcin: the hormonal link between bone and reproduction
    Gerard Karsenty; Fiscal Year: 2013
    ..In the second aspect of this work we then showed that the osteoblast-derived hormone osteocalcin favors glucose handling by the body and enhances energy homeostasis...
  19. CSF-1 in Dental Biology
    SHERRY L ABBOUD-WERNER; Fiscal Year: 2012
    ..op/op mice expressing either csCSF-1 (op/opCS) or sCSF-1 (op/opS) in odontoblasts under the control of the osteocalcin (OC) promoter were generated and showed distinct tooth phenotypes...
  20. Osteocalcin as a regulator of energy metabolism
    Gerard Karsenty; Fiscal Year: 2012
    ..Looking for substrates of the Esp gene product we noted that Osteocalcin-deficient mice had a phenotype that is the mirror image of the one observed in Esp-deficient mice...
  21. Evaluation of Recombinant Human Lactoferrin as an Osteogenic Biomaterial
    Ashley A Amini; Fiscal Year: 2013
    ..Alizarin Red will be utilized to study the degree of mineralization after 21 days of culture. Additionally, osteocalcin, osteopontin and Runx2, alkaline phosphatase expression levels will be assessed to study the effects of the ..
  22. SYNTHESIS OF OSTEOCALCIN IN BONE
    Jane B Lian; Fiscal Year: 2013
    This program continues to study the tissue specific and developmental regulation of the osteocalcin (OC) gene and the factors that contribute to induction and progression of osteoblast (OB) maturation...
  23. Black Bear Parathyroid Hormone as an Anabolic Agent for Bone
    SETH WARNER DONAHUE; Fiscal Year: 2011
    ..by endogenous parathyroid hormone (PTH), since serum PTH levels are correlated with the bone formation marker osteocalcin in hibernating and active bears...
  24. Bone development and the IGF1 axis in preterm infants given massage intervention.
    Shannon L Haley; Fiscal Year: 2012
    ..mineral content (DEXA) and bone strength (tibia speed of sound), greater levels of bone formation biomarkers (osteocalcin &bone specific alkaline phosphotase) and lower levels of bone resorption markers (urinary pyridinium ..
  25. NIDCR Individual Predoctoral Dental Scientist Fellowship
    JOSHUA PADOVANO; Fiscal Year: 2013
    ..The mouse models are designed using the osteocalcin (OC) and dentin sialophosphoprotein (DSPP) gene promoters to drive tissue-specific overexpression in bone and ..
  26. Growth Factors;Effect on Aspects of Bone Formation
    Ernesto Canalis; Fiscal Year: 2012
    ..In addition, transgenic mice overexpressing Nov under the control of the osteocalcin promoter exhibited osteopenia secondary to decreased bone formation...
  27. KROX 20 AND BONE MASS
    Baruch Frenkel; Fiscal Year: 2010
    ..transcriptional activity were strongly inhibited by glucocorticoids (GCs), contributing to the repression of osteocalcin transcription in cultured osteoblasts...
  28. Renal Osteodystrophy and Vascular Calcification
    Keith A Hruska; Fiscal Year: 2013
    ..One of the new hormones regulates energy utilization and the effects of changes in osteocalcin on cardiac energy utilization will be studied in aim 2...
  29. Endothelial-to-osteoblast transition in prostate cancer bone metastasis
    Sue Hwa Lin; Fiscal Year: 2013
    ..bone metastasis specimens, we detected stromal cells that co-express osteoblast and endothelial cell markers, osteocalcin and Tie2, respectively...
  30. The Skeletal Effects of Reducing Inflammation in Type 2 Diabetes Mellitus
    Mishaela R Rubin; Fiscal Year: 2010
    ..cellular products of the osteoblast, the cell in bone that directs processes associated with bone formation, is osteocalcin. It has been shown recently that osteocalcin participates in glucose homeostasis by signaling pancreatic cells ..
  31. Role of Endothelial Progenitor Cells in vascular Aging and Calcification
    Amir Lerman; Fiscal Year: 2013
    ..percentage of EPCs expressing an osteogenic phenotype, as assessed by staining for the bone- related protein, osteocalcin (OCN), has led us to propose the overall novel concept that expression of an osteogenic phenotype by ..
  32. Biochemical markers of bone turnover in metastatic prostate cancer
    Primo N Lara; Fiscal Year: 2010
    ..We found that several baseline markers of bone formation (osteocalcin, procollagen N-terminal propeptides: PINP &PIIINP, total alkaline phosphates) and resorption (N-telopeptide,..
  33. Effects of Osteocalcin and Osteopontin on Damage Morphology and Bone Fragility
    Deepak Vashishth; Fiscal Year: 2013
    ..Studies conducted under the parent grant suggest that non-collagenous matrix (NCPs) proteins including osteocalcin (OC) and osteopontin (OPN) act as link proteins to bone mineral and influence the initiation of bone fracture ..
  34. Crevicular Fluid Content During Early Wound Healing- Implant versus Tooth
    Binnaz Leblebicioglu; Fiscal Year: 2010
    ..g. VEGF, TGF-b, IL-6, IL-10, MMP-8, MMP-9, TIMP-1, TIMP-2, TNF-a, IL-1b, bone alkaline phosphatase and osteocalcin) to determine: a) anticipated differences in early wound healing responses at soft and/or hard tissue levels ..
  35. Analyzing the Role of Wnt Signaling in Bone Development
    Bart O Williams; Fiscal Year: 2013
    ..Subaims will include characterizing the phenotypes associated with Osteocalcin-cre- mediated conditional deletion of Wls in osteoblasts, assessing the function in Wls in osteoblasts of ..
  36. Mechanistic Role of NELL-1 in Premature Suture Closure
    Kang Ting; Fiscal Year: 2003
    ..Microarrays show up-regulation of osteoblast differentiation marker like osteopontin and osteocalcin. To further delineate the in vivo effects of NELL-1, we have constructed NELL-1 over-expression transgenic mice ..
  37. VITAMIN D HORMONE MECHANISM OF ACTION IN CULTURED CELLS
    MARK HAUSSLER; Fiscal Year: 1999
    ..of the human vitamin D receptor (hVDR), and elucidated the vitamin D response element (VDRE) in the rat osteocalcin gene...
  38. ACTION OF DMSA ON CELLULAR LEAD METABOLISM AND TOXICITY
    Joel Pounds; Fiscal Year: 1993
    ..and from which subcellular kinetic pool, to provide restoration of biochemical function (porphyrin, heme, and osteocalcin production) to the cells? together, these experiments will test the hypothesis that DMSA will decrease the ..
  39. BONE RESPONSE TO COMBINED MECHANICAL AND PTH STIMULATION
    X Guo; Fiscal Year: 2005
    ..Specific Aim 1: To determine the changes in gene expression (c-fos, cbfa-1, IGF-1, collagen a1(I), osteopontin, osteocalcin, alkaline phosphatase, RANK-L, and osteoprotegerin) by RT-PCR and in situ hybridization, bone cell activities ..
  40. VITAMIN K AND SKELETAL HEALTH
    Neil Binkley; Fiscal Year: 2000
    ..time), it has recently become appreciated that measurement of undercarboxylation of other proteins, notably osteocalcin, is a much more sensitive measure of adequacy...
  41. BASIC FIBROBLAST GROWTH FACTOR EXPRESSION IN BONE CELLS
    Marja Hurley; Fiscal Year: 2003
    ..of differentiation of early and mature osteoblasts including, CBFA1, type 1 collagen, alkaline phosphatase and osteocalcin. We will examine the expression of genes which support osteoblast differentiation which may be downstream of ..
  42. COMPLEMENTARY DNA PROBES TO A STEROID RECEPTOR GENE
    J Pike; Fiscal Year: 1992
    ..by employing natural and cloned target gene promoters as reporter genes for transcriptional activity (CaBP, osteocalcin, MMTV)...
  43. TRANSCRIPTIONAL REGULATION BY FIBROBLAST GROWTH FACTOR
    Dwight Towler; Fiscal Year: 2001
    ..interactions and kinase cascades conferring FGFR1/FGFR2-regulated gene expression in osteoblasts, using osteocalcin (OC) and interstitial collagenase (MMP) FGF response elements (FREs) as models for study...
  44. CHEMOATTRACTANTS & GROWTH FACTORS IN MINERALIZED TISSUES
    Peter Hauschka; Fiscal Year: 1991
    ..The chemoattractant work focuses on bone and dentin matrix proteins, particularly osteocalcin and its constituent peptides which are active on monocytes in vitro...
  45. LEAD EXPOSURE, GENETICS AND OSTEOPOROSIS EPIDEMIOLOGY
    Susan Korrick; Fiscal Year: 2002
    ..is associated with declines in bone mineral density (BMD) and increases in bone turnover (defined by serum osteocalcin and urinary cross-linked N telopeptide of type I collagen or NTX) in middle-aged women...
  46. LEAD EXPOSURE, GENETICS AND OSTEOPOROSIS EPIDEMIOLOGY
    Susan Korrick; Fiscal Year: 1999
    ..is associated with declines in bone mineral density (BMD) and increases in bone turnover (defined by serum osteocalcin and urinary cross-linked N telopeptide of type I collagen or NTX) in middle-aged women...
  47. METABOLISM OF NONCOLLAGENOUS BONE PROTEINS
    SATORU NISHIMOTO; Fiscal Year: 1992
    ..what is the biological function of the vitamin K-dependent, bone specific protein, bone Gla protein (BGP, Osteocalcin)? BGP is an abundant, noncollagenous protein which comprises approximately 1-2% of the total proteins present...
  48. Mechanosensing function of primary cilium-polycysin complex in bone
    Zhousheng Xiao; Fiscal Year: 2010
    ..mice have impaired bone and osteoblastic response to mechanical loading in vivo and in vitro and that the Osteocalcin-Cre mediated selective deletion of PKD1 from bone results in osteopenia in adult mice, we propose that the ..
  49. MECHANISMS OF GENE SELECTIVITY IN VITAMIN D SIGNALING
    J Pike; Fiscal Year: 2001
    ..of vitamin D3 are integrated with other incoming extracellular signals to regulate the expression of human osteocalcin and 24OHase genes...
  50. Vitamin K and Bone in Children with Cerebral Palsy
    CHRISTOPHER MODLESKY; Fiscal Year: 2007
    ..Vitamin K is necessary for the carboxylation of osteocalcin. Undercarboxylation of osteocalcin is associated with poor mineralization of the skeleton...
  51. Molecular Mechanisms of Bone Formation
    Yi Ping Li; Fiscal Year: 2006
    ..CNBP directly regulates osteocalcin expression and overexpression of CNBP increases osteoblast proliferation in vitro...
  52. CIRCADIAN MECHANISMS REGULATING OSTEOGENESIS
    Jeffrey Gimble; Fiscal Year: 2005
    ..Clues in the literature suggest that a peripheral, "osteogenic" circadian clock exists; serum levels of osteocalcin, alkaline phosphatase, and C- telopeptide exhibit a reproducible, oscillatory diurnal pattern in human subjects ..
  53. TGF-Beta regulation of Runx2 in bone formation and quality
    Tamara Alliston; Fiscal Year: 2007
    ..Specifically, the repression of Runx2 by Smad3/HDAC4/5 confers TGF-[unreadable] inhibitory signals to osteocalcin and Runx2 expression, and to osteoblast differentiation in vitro...
  54. MOLECULAR MECHANISMS OF GENETIC REGULATION OF BONE MASS
    John Eisman; Fiscal Year: 1993
    ..This hypothesis is supported by our recent finding that the strong genetic influence on circulating osteocalcin levels is mediated at least in part through allelic variation in the vitamin D receptor (VDR) gene as detected ..
  55. AD OC TK/VAL GENE THERAPY CLINICAL CORRELATES
    Leland Chung; Fiscal Year: 2001
    ..Laboratory evidence suggests that both cancer epithelium and its supporting stroma express a common protein, osteocalcin (OC)...
  56. BIOMARKERS OF AGING--CIRCULATING/DEPOSITED OSTEOCALCIN
    PAUL GALLOP; Fiscal Year: 1992
    b>Osteocalcin (OC), a vitamin K-dependent protein is made specifically by osteoblasts...
  57. CHARACTERIZATION OF VITAMIN D3 RECEPTOR BINDING TO DNA
    MARILEE PARSONS; Fiscal Year: 1992
    ..In initial experiments, VDR binding to the promoter region of the osteocalcin gene will be used as a model system...
  58. Anabolic Effect of Osteogenic Peptides
    Gary Balian; Fiscal Year: 2009
    ..We have shown this by measuring increases in alkaline phosphatase activity and gene expression for osteocalcin, bone sialoprotein, osterix and RUNX2, moreover, the peptides promote bone repair in critical sized unicortical ..
  59. INVESTIGATION OF THE RECEPTOR FOR OSTEOCALCIN
    PATRICIA MACCHIARULO; Fiscal Year: 1991
    ..These cells are under the exquisite control of numerous humoral and local factors. Osteocalcin (bone Gla protein, BGP) is one of the predominant proteins in bone, comprising 10-20% of the noncollagenous ..
  60. RAPID MOLECULAR ACTIONS OF 1-A 25-DIHYDROXYVITAMIN D3
    DANIEL BARAN; Fiscal Year: 1999
    ..The physiologic role of the rapid effects is demonstrated by their modulation of hormone-induced increments in osteocalcin (OC) gene transcription in osteoblast-like cells...
  61. HOXBOXES, HOMEODOMAINS, AND OSTEOBLAST GENE EXPRESSION
    Dwight Towler; Fiscal Year: 2007
    ..Msx2 suppresses osteocalcin (OC) gene expression via (a) a Ku antigen complex that enhances Runx2-dependent OC transcription; and (b) MINT (..
  62. EFFECT OF PB2+ TOXICITY AT THE MOLECULAR LEVEL
    TERRY DOWD; Fiscal Year: 2001
    ..Some of the properties of a noncollagenous protein secreted from the osteoblast, osteocalcin, have been found to be altered by Pb2+. Osteocalcin plays a role in bone mineralization as well as resorption...
  63. REGULATION OF OSTEOCALCIN CONCENTRATIONS IN BLOOD
    Caren Gundberg; Fiscal Year: 1991
    b>Osteocalcin is an abundant calcium binding protein indigenous to the organic matrix of bone. Development of radioimmunoassays for osteocalcin revealed that it circulates in blood...