blocking antibodies

Summary

Summary: Antibodies that inhibit the reaction between ANTIGEN and other antibodies or sensitized T-LYMPHOCYTES (e.g., antibodies of the IMMUNOGLOBULIN G class that compete with IGE antibodies for antigen, thereby blocking an allergic response). Blocking antibodies that bind tumors and prevent destruction of tumor cells by CYTOTOXIC T-LYMPHOCYTES have also been called enhancing antibodies. (Rosen et al., Dictionary of Immunology, 1989)

Top Publications

  1. ncbi Established T cell-driven germinal center B cell proliferation is independent of CD28 signaling but is tightly regulated through CTLA-4
    Lucy S K Walker
    Department of Pathology, University of California, San Francisco, CA 94143, USA
    J Immunol 170:91-8. 2003
  2. ncbi Mouse strain differences in plasmacytoid dendritic cell frequency and function revealed by a novel monoclonal antibody
    Carine Asselin-Paturel
    Laboratory for Immunological Research, Schering Plough Research Institute, Dardilly, France
    J Immunol 171:6466-77. 2003
  3. ncbi Involvement of cellular death in TRAIL/DR5-dependent suppression induced by CD4(+)CD25(+) regulatory T cells
    X Ren
    Institute for Immunobiology and Department of Immunology, Shanghai Medical College, Fudan University, Shanghai, China
    Cell Death Differ 14:2076-84. 2007
  4. ncbi Function blocking antibodies to neuropilin-1 generated from a designed human synthetic antibody phage library
    Wei Ching Liang
    Antibody Engineering, Genentech Inc, South San Francisco, CA 94080, USA
    J Mol Biol 366:815-29. 2007
  5. ncbi IgE-mediated allergen presentation and blocking antibodies: regulation of T-cell activation in allergy
    R J J van Neerven
    Department of Dermatology and Allergology, University Medical Center Utrecht, Utrecht, The Netherlands
    Int Arch Allergy Immunol 141:119-29. 2006
  6. ncbi Function-blocking antibodies to human vascular adhesion protein-1: a potential anti-inflammatory therapy
    Christopher M Kirton
    Immunology Division, Department of Pathology, Cambridge University, Cambridge, UK
    Eur J Immunol 35:3119-30. 2005
  7. ncbi Prevalence and functional significance of thyrotropin receptor blocking antibodies in children and adolescents with chronic lymphocytic thyroiditis
    Shiri B Feingold
    Division of Endocrinology, Children s Hospital Boston, 300 Longwood Avenue, Boston, Massachusetts 02115, USA
    J Clin Endocrinol Metab 94:4742-8. 2009
  8. ncbi Characterization of thyroid-stimulating blocking antibodies that appeared during transient hypothyroidism after radioactive iodine therapy
    A W Kung
    Department of Medicine, University of Hong Kong, Queen Mary Hospital, Hong Kong
    Thyroid 10:909-17. 2000
  9. pmc IgG-blocking antibodies inhibit IgE-mediated anaphylaxis in vivo through both antigen interception and Fc gamma RIIb cross-linking
    Richard T Strait
    Division of Emergency Medicine, Cincinnati Children s Hospital Medical Center, Cincinnati, Ohio, USA
    J Clin Invest 116:833-41. 2006
  10. ncbi Cutaneous side-effects of kinase inhibitors and blocking antibodies
    Caroline Robert
    Department of Medical Oncology, Dermatology Unit, Institut Gustave Roussy, 39 rue Camille Desmoulins, 94801Villejuif Cedex, France
    Lancet Oncol 6:491-500. 2005

Detail Information

Publications242 found, 100 shown here

  1. ncbi Established T cell-driven germinal center B cell proliferation is independent of CD28 signaling but is tightly regulated through CTLA-4
    Lucy S K Walker
    Department of Pathology, University of California, San Francisco, CA 94143, USA
    J Immunol 170:91-8. 2003
    ..By examining germinal center responses in mice where the ability to signal through T cell CTLA-4 was compromised, we provide data that supports a critical role for CTLA-4 in down-regulating T cell help for germinal center B cells...
  2. ncbi Mouse strain differences in plasmacytoid dendritic cell frequency and function revealed by a novel monoclonal antibody
    Carine Asselin-Paturel
    Laboratory for Immunological Research, Schering Plough Research Institute, Dardilly, France
    J Immunol 171:6466-77. 2003
    ..The higher ability of 129Sv mice to produce IFN-alpha in vivo is related to a higher number of pDC, but also to a higher ability of pDC from 129Sv mice to produce IFN-alpha in vitro in response to viral stimulation...
  3. ncbi Involvement of cellular death in TRAIL/DR5-dependent suppression induced by CD4(+)CD25(+) regulatory T cells
    X Ren
    Institute for Immunobiology and Department of Immunology, Shanghai Medical College, Fudan University, Shanghai, China
    Cell Death Differ 14:2076-84. 2007
    ..The prolonged survival of allogeneic skin grafts induced by Treg was inhibited by DR5-blocking antibodies. Our findings suggest that the TRAIL/DR5 pathway is one of the mechanisms used by Treg to regulate immune ..
  4. ncbi Function blocking antibodies to neuropilin-1 generated from a designed human synthetic antibody phage library
    Wei Ching Liang
    Antibody Engineering, Genentech Inc, South San Francisco, CA 94080, USA
    J Mol Biol 366:815-29. 2007
    ..To investigate the possibility of function blocking antibodies to NRP1 as potential therapeutics, and study the consequence of targeting NRP1 in murine tumor models, ..
  5. ncbi IgE-mediated allergen presentation and blocking antibodies: regulation of T-cell activation in allergy
    R J J van Neerven
    Department of Dermatology and Allergology, University Medical Center Utrecht, Utrecht, The Netherlands
    Int Arch Allergy Immunol 141:119-29. 2006
    ....
  6. ncbi Function-blocking antibodies to human vascular adhesion protein-1: a potential anti-inflammatory therapy
    Christopher M Kirton
    Immunology Division, Department of Pathology, Cambridge University, Cambridge, UK
    Eur J Immunol 35:3119-30. 2005
    ..Furthermore, the chimeric antibodies prevented leukocyte adhesion and transmigration in vitro and in vivo. Hence, these chimeric antibodies have the potential to be used as a new anti-inflammatory therapy...
  7. ncbi Prevalence and functional significance of thyrotropin receptor blocking antibodies in children and adolescents with chronic lymphocytic thyroiditis
    Shiri B Feingold
    Division of Endocrinology, Children s Hospital Boston, 300 Longwood Avenue, Boston, Massachusetts 02115, USA
    J Clin Endocrinol Metab 94:4742-8. 2009
    TSH receptor (TSHR) blocking antibodies (Abs) inhibit TSH-induced thyroid growth and function in some adults with chronic lymphocytic thyroiditis (CLT), but their role in the pediatric age range is unknown.
  8. ncbi Characterization of thyroid-stimulating blocking antibodies that appeared during transient hypothyroidism after radioactive iodine therapy
    A W Kung
    Department of Medicine, University of Hong Kong, Queen Mary Hospital, Hong Kong
    Thyroid 10:909-17. 2000
    ..hypothyroidism had a surge in TBII activity and all except one became positive for thyroid-stimulating blocking antibodies (TSBAb), as measured by inhibition of TSH-stimulated cAMP from WT cells...
  9. pmc IgG-blocking antibodies inhibit IgE-mediated anaphylaxis in vivo through both antigen interception and Fc gamma RIIb cross-linking
    Richard T Strait
    Division of Emergency Medicine, Cincinnati Children s Hospital Medical Center, Cincinnati, Ohio, USA
    J Clin Invest 116:833-41. 2006
    ....
  10. ncbi Cutaneous side-effects of kinase inhibitors and blocking antibodies
    Caroline Robert
    Department of Medical Oncology, Dermatology Unit, Institut Gustave Roussy, 39 rue Camille Desmoulins, 94801Villejuif Cedex, France
    Lancet Oncol 6:491-500. 2005
    ..Elucidation of this connection could lead to the identification of crucial predictive factors for tumour response...
  11. pmc The human immune response to Plasmodium falciparum includes both antibodies that inhibit merozoite surface protein 1 secondary processing and blocking antibodies
    Roseangela I Nwuba
    Cellular Parasitology Programme, Department of Zoology, University of Ibadan, Ibadan, Nigeria
    Infect Immun 70:5328-31. 2002
    ..There was no correlation between the titer of the antibody to the 19-kDa fragment of MSP1 and its inhibitory activity. These findings have implications for the design of MSP1-based vaccines...
  12. ncbi Blocking antibodies induced by specific allergy vaccination prevent the activation of CD4+ T cells by inhibiting serum-IgE-facilitated allergen presentation
    R J Van Neerven
    ALK ABELLO, Horsholm, Denmark and Lung and Allergy Clinic, Copenhagen, Denmark
    J Immunol 163:2944-52. 1999
    ....
  13. ncbi Intravenous immunoglobulins neutralize blocking antibodies in Guillain-Barré syndrome
    Brigitte Buchwald
    Department of Neurology, Julius Maximilians Universitat, Josef Schneider Strasse 11, 97080 Wurzburg, Germany
    Ann Neurol 51:673-80. 2002
    ..We attempted to delineate the effect of IVIg on neuromuscular blocking antibodies in GBS...
  14. ncbi Analysis of B-cell epitopes from the allergen Hev b 6.02 revealed by using blocking antibodies
    Martha Pedraza-Escalona
    Instituto de Quimica, Universidad Nacional Autonoma de Mexico, Circuito Exterior, Cd Universitaria, Coyoacan, Mexico, D F 04510, Mexico
    Mol Immunol 46:668-76. 2009
    ..02-IgE interactions. Antibodies recognizing this important allergen have potential to be used to diagnose and ultimately treat latex allergy...
  15. ncbi IL-10 underlies distinct susceptibility of BALB/c and C57BL/6 mice to Mycobacterium avium infection and influences efficacy of antibiotic therapy
    Susana Roque
    Life and Health Sciences Research Institute, School of Health Sciences, University of Minho, Campus Gualtar, 4710 057 Braga, Portugal
    J Immunol 178:8028-35. 2007
    ..This should now be considered in the context of human response to mycobacterial infection, particularly as a possible strategy to improve treatment against infections by mycobacteria...
  16. pmc Two amino acid substitutions within the first external loop of CCR5 induce human immunodeficiency virus-blocking antibodies in mice and chickens
    Claudia Pastori
    Infectious Diseases Clinic, San Raffaele Scientific Institute, Milan, Italy
    J Virol 82:4125-34. 2008
    ..These findings may be relevant to the induction of strong and efficient HIV-blocking antibodies.
  17. ncbi Inhibitory and blocking monoclonal antibody epitopes on merozoite surface protein 1 of the malaria parasite Plasmodium falciparum
    C Uthaipibull
    Division of Parasitology, Walter Reed Army Institute of Research, Washington, DC, USA
    J Mol Biol 307:1381-94. 2001
    ..The incorporation of several specific amino acid changes enabled the design of proteins that bound inhibitory but not blocking antibodies. These may be suitable for the development of MSP-1-based vaccines against malaria.
  18. pmc HIV blocking antibodies following immunisation with chimaeric peptides coding a short N-terminal sequence of the CCR5 receptor
    Benjamin M Chain
    Division of Infection and Immunity, Windeyer Building, UCL, 46 Cleveland St, London W1T 4JF, UK
    Vaccine 26:5752-9. 2008
    ..Thus results of this study suggest that N-terminal fragments of CCR5 may provide potential immunogens with which to generate blocking antibodies to this receptor, while avoiding the dangers of including T cell auto-epitopes.
  19. pmc Integrin-blocking antibodies delay keratinocyte re-epithelialization in a human three-dimensional wound healing model
    Christophe Egles
    Division of Cancer Biology and Tissue Engineering, Department of Oral and Maxillofacial Pathology, School of Dental Medicine, Tufts University, Boston, Massachusetts, United States of America
    PLoS ONE 5:e10528. 2010
    ..These results demonstrate beta4 adhesion-blocking antibodies may have adverse effects on normal tissue, whereas antibodies directed to other epitopes may provide safer ..
  20. ncbi Blocking antibodies in blood from patients with recurrent spontaneous abortion in relation to pregnancy outcome and intravenous immunoglobulin treatment
    B Jablonowska
    Division of Obstetrics and Gynecology, University Hospital, Linkoping, Sweden
    Am J Reprod Immunol 45:226-31. 2001
    To study whether the occurrence of mixed lymphocyte culture (MLC) blocking antibodies is associated with pregnancy outcome in women with unexplained recurrent spontaneous abortion (RSA) and the in vivo effect of intravenous ..
  21. ncbi Induction of CD4 T cell changes in murine AIDS is dependent on costimulation and involves a dysregulation of homeostasis
    Michael H Yen
    Department of Biology, University of California at San Diego, La Jolla, CA 92093, USA
    J Immunol 169:722-31. 2002
    ..Thus, a combination of up-regulation of costimulatory ligands and disruption of homeostatic control may be responsible for CD4 lymphoproliferation in MAIDS...
  22. ncbi Identification of tissue transglutaminase as a novel molecule involved in human CD8+ T cell transendothelial migration
    Karkada Mohan
    Departments of Pediatrics, Microbiology Immunology and Pathology, Dalhousie University, and Institute for Marine Biosciences, National Research Council, Halifax, Nova Scotia, Canada
    J Immunol 171:3179-86. 2003
    ..Thus, 6B9 is a new blocking mAb to human tTG, which demonstrates that tTG may have a novel role in mediating CD8(+) T cell migration across cytokine-activated endothelium and infiltration of tissues during inflammation...
  23. ncbi Viral abrogation of stem cell transplantation tolerance causes graft rejection and host death by different mechanisms
    Daron Forman
    Program in Immunology, Department of Medicine, University of Massachusetts Medical School, Worcester, MA 01655, USA
    J Immunol 168:6047-56. 2002
    ..Clinical application of stem cell transplantation protocols based on costimulation blockade and tolerance induction may require patient isolation to facilitate the procedure and to protect recipients...
  24. ncbi Two-signal blockade with anti-CD45RB and anti-CD154 monoclonal antibodies inhibits graft rejection via CD4-dependent mechanisms in allogeneic skin transplantation
    Eun Young Kim
    Transplantation Research Center, Sungkyunkwan University School of Medicine, Seoul 135 710, Korea
    Exp Mol Med 38:284-94. 2006
    ..In addition, results of IL-4 and IL-10 production were also showed CD4-dependence. Therefore, the two- signal blockade is accompanied by CD4-dependent mechanisms in allogeneic skin transplantation...
  25. ncbi Successful allogeneic neonatal bone marrow transplantation devoid of myeloablation requires costimulatory blockade
    Brian W Soper
    The Jackson Laboratory, Bar Harbor, ME 04609, USA
    J Immunol 171:3270-7. 2003
    ..This combinatorial approach provides a nontoxic method to establish tolerant allogeneic engraftment for treatment of progressive childhood diseases...
  26. ncbi In vivo CD86 blockade inhibits CD4+ T cell activation, whereas CD80 blockade potentiates CD8+ T cell activation and CTL effector function
    Thomas J Lang
    Research Service, Baltimore Veterans Affairs Medical Center, and Division of Rheumatology and Clinical Immunology, University of Maryland School of Medicine, Baltimore, MD 21201, USA
    J Immunol 168:3786-92. 2002
    ..In contrast, CD86 is critical for the activation of naive CD4(+) T cells in either a Th1 or a Th2 cytokine-mediated response...
  27. ncbi T cell immunity to lymphoma following treatment with anti-CD40 monoclonal antibody
    Alison L Tutt
    Tenovus Research Laboratory, Cancer Sciences Division, University School of Medicine, Southampton General Hospital, Southampton, United Kingdom
    J Immunol 168:2720-8. 2002
    ..These data underline the ability of anti-CD40 mAb to potentiate CTL responses and the potency of cellular immunity in eradicating large quantities of syngeneic tumor...
  28. ncbi CTLA-4 blockade differentially influences the outcome of non-lethal and lethal Plasmodium yoelii infections
    Bernd Lepenies
    Department of Immunology, Bernhard Nocht Institute for Tropical Medicine, Bernhard Nocht Str 74, 20359 Hamburg, Germany
    Microbes Infect 9:687-94. 2007
    ..In contrast, blockade of CTLA-4 during infection with a P. yoelii strain exhibiting a higher parasitemia induced markedly increased serum-levels of TNF-alpha, which was associated with severe inflammation and reduced survival...
  29. ncbi Without CD4 help, CD8 rejection of pig xenografts requires CD28 costimulation but not perforin killing
    Y Zhan
    Walter and Eliza Hall Institute of Medical Research, Parkville, Australia
    J Immunol 167:6279-85. 2001
    ..Therefore, we show that CD8 cells are activated to mediate FPP rejection independent of perforin and that this CD4-independent activation of CD8 cells critically depends on B7/CD28 costimulation...
  30. ncbi Dynamics of pathogenic and suppressor T cells in autoimmune diabetes development
    Silvia Gregori
    BioXell, Milan, Italy
    J Immunol 171:4040-7. 2003
    ..Thus, the pathogenesis of autoimmune diabetes in NOD mice is correlated with both an enhanced pathogenicity of CD4(+)CD25(-) T cells and a decreased suppressive activity of CD4(+)CD25(+) T cells...
  31. ncbi Interference with CD28, CD80, CD86 or CD152 in collagen-induced arthritis. Limited role of IFN-gamma in anti-B7-mediated suppression of disease
    A C Tellander
    Active Biotech Research AB, SE-22007 Lund, Sweden
    J Autoimmun 17:39-50. 2001
    ..We also demonstrate that the enhanced production of IFN-gamma observed after B7-blockade is not accountable for the anti-B7 mediated inhibition of CIA...
  32. ncbi Negative regulation of phagocytosis in macrophages by the CD47-SHPS-1 system
    Hideki Okazawa
    Biosignal Research Center, Institute for Molecular and Cellular Regulation, and Department of Dermatology, Graduate School of Medicine, Gunma University, Showa machi, Maebashi, Gunma, Japan
    J Immunol 174:2004-11. 2005
    ..The inhibition of phagocytosis by the SHPS-1-SHP-1 complex may be mediated at the level of Syk or PI3K signaling...
  33. ncbi Blockade of CD86 signaling facilitates a Th2 bias at the maternal-fetal interface and expands peripheral CD4+CD25+ regulatory T cells to rescue abortion-prone fetuses
    Xiao Yong Zhu
    Laboratory of Reproductive Immunology, Institute of Obstetrics and Gynecology, Fudan University, Shanghai 200011, China
    Biol Reprod 72:338-45. 2005
    ..These results suggest that selective blockade of CD86 costimulation leads to maternal immune tolerance to embryo antigen, and might contribute to a rational immunoregulatory regimen for recurrent spontaneous abortion...
  34. ncbi Blockade of the interaction between PD-1 and PD-L1 accelerates graft arterial disease in cardiac allografts
    Noritaka Koga
    Department of Cardiovascular Medicine, Tokyo Medical and Dental University, Tokyo, Japan
    Arterioscler Thromb Vasc Biol 24:2057-62. 2004
    ..PD-1 is involved in the negative regulation of some immune responses. We evaluated the role of PD-ligand 1 (PD-L1) in graft arterial disease (GAD) of cardiac allografts and in smooth muscle cells (SMCs)...
  35. ncbi Activation of APCs through CD40 or Toll-like receptor 9 overcomes tolerance and precipitates autoimmune disease
    Hiroshi T Ichikawa
    Department of Neurology, University of Rochester School of Medicine and Dentistry, NY 14642, USA
    J Immunol 169:2781-7. 2002
    ..Microbial pathogens could 1) release molecules that bind Toll-like receptors, and/or 2) stimulate microbe-specific T cells to express CD40 ligand, thereby licensing APCs that bear both microbial and autoantigens to break tolerance...
  36. ncbi Blockade of B7-H1 on macrophages suppresses CD4+ T cell proliferation by augmenting IFN-gamma-induced nitric oxide production
    Tomohide Yamazaki
    Department of Immunology, Juntendo University School of Medicine, Hongo, Tokyo, Japan
    J Immunol 175:1586-92. 2005
    ....
  37. ncbi B7-1 costimulatory molecule is critical for the development of experimental autoimmune myasthenia gravis
    Mathilde A Poussin
    Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX 77555, USA
    J Immunol 170:4389-96. 2003
    ..The data also point to B7-2 molecules as being the dominant costimulatory molecules required for AChR-induced lymphocyte proliferation...
  38. ncbi B7-H1 (programmed death-1 ligand) on dendritic cells is involved in the induction and maintenance of T cell anergy
    Nicole Selenko-Gebauer
    Institute of Immunology, University of Vienna Medical School, Vienna, Austria
    J Immunol 170:3637-44. 2003
    ..Our results thus demonstrate that the mAb DF272-defined surface molecule B7-H1 represents a unique receptor structure on DC that might play a role in the induction and maintenance of T cell anergy...
  39. ncbi Activation of alloreactive CD8+ T cells operates via CD4-dependent and CD4-independent mechanisms and is CD154 blockade sensitive
    Yuan Zhai
    Dumont University of California, Los Angeles, Transplant Center, Division of Liver and Pancreas Transplantation, Department of Surgery, School of Medicine, 90095, USA
    J Immunol 170:3024-8. 2003
    ..Indeed, CD154 blockade was effective in preventing CD8(+) T cell-mediated cardiac allograft rejection...
  40. ncbi Blockade of CD40-mediated signaling is sufficient for inducing islet but not skin transplantation tolerance
    Nancy E Phillips
    Department of Medicine, University of Massachusetts Medical School, Worcester, MA 01655, USA
    J Immunol 170:3015-23. 2003
    ..We speculate that a requirement for regulatory CD4(+) T cells in skin allograft recipients could account for this differential response to tolerance induction...
  41. ncbi CD137-deficient mice have reduced NK/NKT cell numbers and function, are resistant to lipopolysaccharide-induced shock syndromes, and have lower IL-4 responses
    Dass S Vinay
    Louisiana State University Eye Center, Louisiana State University Health Sciences Center School of Medicine, New Orleans, LA 70112, USA
    J Immunol 173:4218-29. 2004
    ....
  42. ncbi The murine NK receptor 2B4 (CD244) exhibits inhibitory function independent of signaling lymphocytic activation molecule-associated protein expression
    Jill M Mooney
    Graduate Program in Immunology, University of Texas Southwestern Medical Center, Dallas 75390, USA
    J Immunol 173:3953-61. 2004
    ..This predominant inhibitory function of murine 2B4 correlates with increased 2B4 long isoform level expression over 2B4 short...
  43. ncbi CTLA-4 blockage increases resistance to infection with the intracellular protozoan Trypanosoma cruzi
    GISLAINE APARECIDA MARTINS
    Department of Immunology, Institute of Biomedical Sciences, University of Sao Paulo, Sao Paulo, Brazil
    J Immunol 172:4893-901. 2004
    ..cruzi by acting in the mechanisms that control IFN-gamma and NO production during the acute phase of the infection...
  44. ncbi Inducible costimulator protein controls the protective T cell response against Listeria monocytogenes
    Hans Willi Mittrücker
    Max Planck Institute for Infection Biology, Schumannstrasse 20 21, 10117 Berlin, Germany
    J Immunol 169:5813-7. 2002
    ..Thus, ICOS costimulation is crucial for protective immunity against the intracellular bacterium L. monocytogenes...
  45. ncbi Induction of autoantigen-specific Th2 and Tr1 regulatory T cells and modulation of autoimmune diabetes
    Cyndi Chen
    Division of Immunology, Beckman Research Institute, City of Hope, Duarte, CA 91010, USA
    J Immunol 171:733-44. 2003
    ..Therefore, treatment of NOD mice with autoantigen could induce Th2 and Tr1 regulatory cells that can suppress the function and/or block the migration of other T cells, including diabetogenic T cells, and inhibit diabetes development...
  46. ncbi Investigation of the role of complement and complement receptors in the modulation of B cell activation by a Paracoccidioides brasiliensis cell wall fraction
    Carla de Agostino Biella
    Departamento de Analises Clinicas, Toxicológicas e Bromatológicas Faculdade de Ciências Farmacêuticas de Ribeirão Preto Universidade de São Paulo, Avenida do Café, S N, B Monte Alegre, CEP 14040 903, Ribeirao Preto, Sao Paulo, Brazil
    Clin Immunol 118:324-31. 2006
    ..These results may contribute for a better understanding of the B cell activation and differentiation processes in response to the F1 fraction from P. brasiliensis...
  47. ncbi Blockade of programmed death-1 engagement accelerates graft-versus-host disease lethality by an IFN-gamma-dependent mechanism
    Bruce R Blazar
    University of Minnesota Cancer Center and Department of Pediatrics, Minneapolis, MN 55455, USA
    J Immunol 171:1272-7. 2003
    ..These data indicate that PD-1 ligation down-regulates GVHD through modulation of IFN-gamma production and suggest a novel therapeutic target for inhibiting GVHD lethality...
  48. ncbi Break of neonatal Th1 tolerance and exacerbation of experimental allergic encephalomyelitis by interference with B7 costimulation
    J Jeremiah Bell
    Department of Molecular Microbiology and Immunology, University of Missouri School of Medicine, Columbia, MO 65212, USA
    J Immunol 171:1801-8. 2003
    ..Thus, recovery from neonatally induced T cell anergy requires B7 molecules to serve double functions, namely, costimulation and induction of cytokine production by APCs...
  49. ncbi ICOS-B7 homologous protein interactions are necessary for mercury-induced autoimmunity
    Yan Zheng
    Department of Microbiology and Immunology, Temple University School of Medicine, Philadelphia, PA 19140, USA
    J Immunol 174:3117-21. 2005
    ..Taken together, these results indicate that the ICOS-B7h costimulation pathway is required for this autoimmune syndrome and suggest that targeting this pathway might have therapeutic benefits for human autoimmune diseases...
  50. ncbi CD28-independent costimulation of T cells in alloimmune responses
    A Yamada
    Laboratory of Immunogenetics and Transplantation and Immunology Research Division, Department of Pathology, Brigham and Women's Hospital and Harvard Medical School, Boston, MA 02115, USA
    J Immunol 167:140-6. 2001
    ....
  51. ncbi Long-term inhibition of murine lupus by brief simultaneous blockade of the B7/CD28 and CD40/gp39 costimulation pathways
    D I Daikh
    Department of Medicine, Department of Veterans Affairs Medical Center, San Francisco, CA 94121, USA
    J Immunol 159:3104-8. 1997
    ..These findings demonstrate that brief simultaneous blockade of the B7/CD28 and CD40/gp39 costimulation pathways can produce benefit that lasts long after treatment has been discontinued...
  52. ncbi CD70 signaling is critical for CD28-independent CD8+ T cell-mediated alloimmune responses in vivo
    Akira Yamada
    Transplantation Research Center, Brigham and Women s Hospital and Children s Hospital Boston, Harvard Medical School, Boston, MA 02115, USA
    J Immunol 174:1357-64. 2005
    ..These novel findings have important implications for the development of transplantation tolerance-inducing strategies in primates and humans, in which CD8(+) T cell depletion is currently mandatory...
  53. ncbi Cutting edge: a critical role for CD70 in CD8 T cell priming by CD40-licensed APCs
    Vadim Y Taraban
    Cancer Sciences Division, Tenovus Research Laboratory, University of Southampton School of Medicine, Southampton General Hospital, Southampton, United Kingdom
    J Immunol 173:6542-6. 2004
    ..Our data indicate that CD70 expression on APCs plays a key role in CD40-dependent CD8 T cell responses...
  54. ncbi Crry/p65, a membrane complement regulatory protein, has costimulatory properties on mouse T cells
    E Fernández-Centeno
    Centro Nacional de Biologia Fundamental, Instituto de Salud Carlos III, Madrid, Spain
    J Immunol 164:4533-42. 2000
    ..The relationship between protective function and costimulation by Crry/p65 is discussed. Our results support a multifunctional role for Crry/p65 in T cells and suggest new links between the natural and adaptive immune responses...
  55. ncbi A critical role for B7/CD28 costimulation in experimental autoimmune encephalomyelitis: a comparative study using costimulatory molecule-deficient mice and monoclonal antibody blockade
    A M Girvin
    Department of Microbiology Immunology, Northwestern University Medical School, Northwestern University Institute for Neuroscience, Chicago, IL 60611, USA
    J Immunol 164:136-43. 2000
    ..Collectively, these results support a critical role for CD28 costimulation in EAE induction...
  56. ncbi WSX-1 and glycoprotein 130 constitute a signal-transducing receptor for IL-27
    Stefan Pflanz
    DNAX Research Institute, Palo Alto, CA 94304, USA
    J Immunol 172:2225-31. 2004
    ..Thus, IL-27 not only contributes to the development of an adaptive immune response through its action on CD4(+) T cells, it also directly acts on cells of the innate immune system...
  57. ncbi CD63 as an activation-linked T cell costimulatory element
    Katharina Pfistershammer
    Institute of Immunology, Medical University of Vienna, Borschkegasse 8a, A 1090 Vienna, Austria
    J Immunol 173:6000-8. 2004
    ..CD63 thus represents an activation-induced reinforcing element, whose triggering promotes sustained and efficient T cell activation and expansion...
  58. ncbi The role of the common cytokine receptor gamma-chain in regulating IL-2-dependent, activation-induced CD8+ T cell death
    Z Dai
    The Carlos and Marguerite Mason Transplantation Research Center, Renal Division, Department of Medicine, Veterans Affairs Medical Center, Emory University, Atlanta, GA 30033, USA
    J Immunol 163:3131-7. 1999
    ..These data provide evidence that IL-2 prepares CD8+ T lymphocytes for AICD by at least two mechanisms: 1) by up-regulating a pro-apoptotic molecule, FasL, and 2) by down-regulating a survival molecule, gamma c...
  59. ncbi Dependency of direct pathway CD4+ T cells on CD40-CD154 costimulation is determined by nature and microenvironment of primary contact with alloantigen
    Andre van Maurik
    Nuffield Department of Surgery, University of Oxford, John Radcliffe Hospital, Oxford, United Kingdom
    J Immunol 172:2163-70. 2004
    ..Taken together, these results clearly demonstrate that naive alloreactive CD4(+) T cells have distinct requirements for CD40-CD154 costimulation depending on the form and microenvironment of primary alloantigen contact...
  60. ncbi Importance of IL-10 for CTLA-4-mediated inhibition of tumor-eradicating immunity
    Vladimir M Jovasevic
    Department of Biochemistry and Molecular Genetics, University of Illinois, Chicago, IL 60612, USA
    J Immunol 172:1449-54. 2004
    ..Taken together, these results indicate that CTLA-4 blockade improves the therapeutic outcome of low-dose L-PAM for MOPC-315 tumor bearers by inhibiting IL-10 secretion as a consequence of blocking CTLA-4 ligation...
  61. ncbi B7 costimulation in the development of lupus: autoimmunity arises either in the absence of B7.1/B7.2 or in the presence of anti-b7.1/B7.2 blocking antibodies
    B Liang
    Department of Internal Medicine, Section of Rheumatology, Yale University School of Medicine, New Haven, CT 06510, USA
    J Immunol 163:2322-9. 1999
    ..These studies indicate that each B7 costimulatory signal may control unique pathological events in murine systemic lupus erythematosus that may not always be apparent in autoantibody titers alone...
  62. ncbi Critical role of OX40 in CD28 and CD154-independent rejection
    Gulcin Demirci
    Department of Medicine, Harvard Medical School, and Division of Immunology, Beth Israel Deaconess Medical Center, Boston, MA 02215, USA
    J Immunol 172:1691-8. 2004
    ..Our study revealed a key cellular mechanism of rejection and identified OX40 as a critical alternative costimulatory molecule in CD28/CD154-independent rejection...
  63. ncbi CD28-independent costimulation of T cells by OX40 ligand and CD70 on activated B cells
    H Akiba
    Department of Immunology, Juntendo University School of Medicine, Tokyo, Japan
    J Immunol 162:7058-66. 1999
    ..These results indicated that OX40L and CD70 expressed on surface Ig- and CD40-stimulated B cells can provide CD28-independent costimulatory signals to T cells...
  64. ncbi B7 requirements for primary and secondary protein- and polysaccharide-specific Ig isotype responses to Streptococcus pneumoniae
    Z Q Wu
    Departments of Pathology and Microbiology and Immunology, Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA
    J Immunol 165:6840-8. 2000
    ..These studies demonstrate a critical, but variable, role for B7-dependent costimulation during an Ig response to an extracellular bacteria...
  65. ncbi CD40 signaling replaces CD4+ lymphocytes and its blocking prevents chronic rejection of heart transplants
    M P Fischbein
    Department of Microbiology, Immunology, and Molecular Genetics, Division of Cardiothoracic Surgery, University of California Los Angeles School of Medicine, Los Angeles, CA 90095, USA
    J Immunol 165:7316-22. 2000
    ..Importantly, this study provides evidence that CD40 ligand-targeted therapies may prevent chronic rejection only in strain combinations where CD4(+) lymphocyte help is absolutely required...
  66. ncbi Ligation of B and T lymphocyte attenuator prevents the genesis of experimental cerebral malaria
    Bernd Lepenies
    Department of Immunology, Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany
    J Immunol 179:4093-100. 2007
    ..berghei ANKA infection of mice and that BTLA is a potential target for therapeutic interventions in severe malaria...
  67. ncbi In vivo helper functions of alloreactive memory CD4+ T cells remain intact despite donor-specific transfusion and anti-CD40 ligand therapy
    Yifa Chen
    Department of Immunology, Cleveland Clinic Foundation, Cleveland, OH 44195, USA
    J Immunol 172:5456-66. 2004
    ....
  68. ncbi In vivo CD40-CD154 (CD40 ligand) interaction induces integrated HIV expression by APC in an HIV-1-transgenic mouse model
    C Chougnet
    Experimental Immunology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    J Immunol 166:3210-7. 2001
    ..Because chronic T cell activation driven by coinfections as well as HIV-1 itself is a characteristic of HIV disease, this pathway may be important in sustaining viral expression from APC reservoirs...
  69. ncbi Blockade of CD40/CD40 ligand interactions attenuates skin fibrosis and autoimmunity in the tight-skin mouse
    K Komura
    Department of Dermatology, Kanazawa University Graduate School of Medical Science, Kanazawa, Japan
    Ann Rheum Dis 67:867-72. 2008
    ..To assess the association of CD40/CD40 ligand (CD40L) interactions with the development of skin fibrosis and autoimmunity in tight-skin (TSK/+) mouse, which is a mouse model for human systemic sclerosis...
  70. ncbi B7/CD28 costimulation is critical in susceptibility to Pseudomonas aeruginosa corneal infection: a comparative study using monoclonal antibody blockade and CD28-deficient mice
    L D Hazlett
    Department of Anatomy Cell Biology, Wayne State University School of Medicine, Detroit, MI 48201, USA
    J Immunol 166:1292-9. 2001
    ..These results support a critical role for B7/CD28 costimulation in susceptibility to P. aeruginosa ocular infection...
  71. ncbi Blockade of B7-H1 suppresses the development of chronic intestinal inflammation
    Takanori Kanai
    Department of Gastroenterology and Hepatology, Graduate School, Tokyo Medical and Dental University, Tokyo, Japan
    J Immunol 171:4156-63. 2003
    ....
  72. ncbi TGF-beta 1 regulates adhesion of mucosal mast cell homologues to laminin-1 through expression of integrin alpha 7
    Anne Rosbottom
    Department of Veterinary Pathology, University of Edinburgh, Roslin, Midlothian, UK EH25 9RG
    J Immunol 169:5689-95. 2002
    ..05), demonstrating a novel role for this molecule in adhesion of a hemopoietic cell to laminin-1...
  73. ncbi Costimulation-dependent modulation of experimental autoimmune encephalomyelitis by ligand stimulation of V alpha 14 NK T cells
    E Pal
    Department of Demyelinating Disease and Aging, National Institute of Neuroscience, National Center of Neurology and Psychiatry, Kodaira, Tokyo, Japan
    J Immunol 166:662-8. 2001
    ..These results have important implications for understanding the role of NK T cells in autoimmunity and for designing a therapeutic strategy targeting NK T cells...
  74. ncbi Induction of vascular smooth muscle cell tenascin-C gene expression by denatured type I collagen is dependent upon a beta3 integrin-mediated mitogen-activated protein kinase pathway and a 122-base pair promoter element
    P L Jones
    Division of Cardiovascular Research, Research Institute, The Hospital for Sick Children, Department of Pediatrics, University of Toronto, Ontario, Canada M5G 1X8
    J Cell Sci 112:435-45. 1999
    ..beta3 integrin function-blocking antibodies attenuate ERK1/2 activation and tenascin-C protein synthesis...
  75. ncbi Epitope mapping of tsh receptor-blocking antibodies in Graves' disease that appear during pregnancy
    A W Kung
    Department of Medicine, University of Hong Kong, Queen Mary, Hong Kong, China
    J Clin Endocrinol Metab 86:3647-53. 2001
    ..All thyroid stimulating-blocking antibodies had blocking activities against residues 261-370 of the C-terminus of the ectodomain...
  76. ncbi Analytical aspects of thyroid antibodies estimation
    David Sinclair
    Department of Clinical Biochemistry, Queen Alexandra Hospital, Portsmouth, UK
    Autoimmunity 41:46-54. 2008
    ..those antibodies recognising the B subunit, located much nearer the cell surface, appear to function as blocking antibodies. The aetiology and mechanics of the autoimmune cellular and antibody responses involves a combination of ..
  77. ncbi The impact of transient hypothyroidism on the increasing rate of congenital hypothyroidism in the United States
    John S Parks
    Division of Pediatric Endocrinology, Department of Pediatrics, Emory University School of Medicine, Atlanta, Georgia, USA
    Pediatrics 125:S54-63. 2010
    ..Among the 4 etiologies for transient hypothyroidism (maternal thyrotropin receptor-blocking antibodies, exposure to maternal antithyroid medications, iodine deficiency, and iodine excess), there is little ..
  78. ncbi [Relationship between OX40/4-1 BB (CD134/CD137) costimulatory molecules expression on T lymphocytes and stimulating and blocking autoantibodies to the TSH-receptor in children with Graves' disease]
    Artur Bossowski
    II Klinika Chorób Dzieci AM w Białymstoku, Białystok
    Endokrynol Diabetol Chor Przemiany Materii Wieku Rozw 11:133-40. 2005
    ..Detection of stimulating and blocking antibodies to the TSH-receptor using JPO9 CHO cells in unfractionated serum were measured by a highly sensitive ..
  79. ncbi [Analysis of costimulatory molecules (CD28-CTLA-4/B7) expression on chosen mononuclear cells in adolescents with Graves' disease during methimazole therapy]
    Artur Bossowski
    II Klinika Chorób Dzieci AM w Białymstoku, Białystok, Poland
    Endokrynol Diabetol Chor Przemiany Materii Wieku Rozw 10:93-101. 2004
    ..Detection of stimulating and blocking antibodies to the TSH-receptor using JPO9 CHO cells in unfractionated serum was measured by a highly sensitive ..
  80. ncbi Clinical value of a new TSH binding inihibitory activity assay using human TSH receptors in the follow-up of antithyroid drug treated Graves' disease. Comparison with thyroid stimulating antibody bioassay
    D Maugendre
    Unité d endocrinologie, Departement de Medecine, CHU, Hopital Sud, 16 Boulevard de Bulgarie, 35043 Rennes Cedex, France
    Clin Endocrinol (Oxf) 54:89-96. 2001
    ..TSAb were also evaluated at each time. Thyroid blocking antibodies (TBAb) were measured in sera positive for h-TBII and negative for TSAb...
  81. ncbi Involvement of laminin binding integrins and laminin-5 in branching morphogenesis of the ureteric bud during kidney development
    R Zent
    Department of Medicine, University of California, San Diego, 9500 Gilman Drive, La Jolla, California 92093 0693, USA
    Dev Biol 238:289-302. 2001
    ..However, inhibiting individual integrin function with blocking antibodies in whole kidney and isolated UB culture only partially inhibited UB branching morphogenesis, suggesting ..
  82. ncbi Iron absorption and transport
    M E Conrad
    USA Cancer Center, University of South Alabama, Mobile 36688, USA
    Am J Med Sci 318:213-29. 1999
    ..Each of these pathways are incompletely described. However, studies with blocking antibodies, observations in rodents with disorders of iron metabolism, and studies in tissue culture cells suggest ..
  83. pmc Sequence variation and immunologic cross-reactivity among Babesia bovis merozoite surface antigen 1 proteins from vaccine strains and vaccine breakthrough isolates
    Tanya LeRoith
    Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164 7040, USA
    Infect Immun 73:5388-94. 2005
    ..surface antigen 1 (MSA-1) is an immunodominant membrane glycoprotein that is the target of invasion-blocking antibodies. While antigenic variation has been demonstrated in MSA-1 among strains from distinct geographical areas, ..
  84. ncbi Basement membrane integrity and keratinization in healthy and ulcerated bovine hoof tissue
    Kay A K Hendry
    Hannah Research Institute, Hannah Research Park, Ayr, KA6 5HL, UK
    J Dairy Res 70:19-27. 2003
    ..relationship between basement membrane anchorage and epidermal keratinization, the effect of function-blocking antibodies to laminins and integrins was tested in tissue explant cultures prepared from healthy sole tissue...
  85. ncbi Roles for integrin very late activation antigen-4 in stroma-dependent erythropoiesis
    N Yanai
    Department of Cell Biology, Tohoku University, Sendai, Japan
    Blood 83:2844-50. 1994
    ..In this stroma-dependent erythropoiesis, we examined the role of adhesion molecules in erythropoiesis by blocking antibodies. The development of the erythroid cells on stroma cells was inhibited by anti-very late activation antigen-..
  86. ncbi Inhibition of complement alternative pathway function with anti-properdin monoclonal antibodies
    R Gupta-Bansal
    Exploratory Research, Gliatech Inc, Cleveland, OH 44122, USA
    Mol Immunol 37:191-201. 2000
    ..These blocking antibodies bind human properdin with high avidity and prevent its interaction with the alternative pathway C3 ..
  87. ncbi Evaluation of serum IgG4 antibodies specific to grass pollen allergen components in the follow up of allergic patients undergoing subcutaneous and sublingual immunotherapy
    Renato E Rossi
    Allergy Unit National Health Service, Rete di Allergologia Regione Piemonte, Cuneo, Italy
    Vaccine 25:957-64. 2007
    A number of reports suggest that induction of IgG 'blocking antibodies' may be important for successful allergen immunotherapy...
  88. ncbi Blockade of IL-2 receptor suppresses HTLV-I and IFN-gamma expression in patients with HTLV-I-associated myelopathy/tropical spastic paraparesis
    Naomi Fukushima
    Department of Molecular Microbiology and Immunology, Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki, Japan
    Intern Med 46:347-51. 2007
    ....
  89. ncbi Pseudomonas aeruginosa exoenzyme S, a bifunctional type-III secreted cytotoxin
    J T Barbieri
    Department of Microbiology and Molecular Genetics, Medical College of Wisconsin, Milwaukee 53226, USA
    Int J Med Microbiol 290:381-7. 2000
    ..of endothelial cells with activated Ras stimulated their motility, while microinjection of Ras-blocking antibodies inhibited cellular motility that is a component of the wound healing process (Fox et al., 1994)...
  90. pmc Division of labor among the alpha6beta4 integrin, beta1 integrins, and an E3 laminin receptor to signal morphogenesis and beta-casein expression in mammary epithelial cells
    J Muschler
    Life Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720, USA
    Mol Biol Cell 10:2817-28. 1999
    ..Signals from laminin for beta-casein expression were inhibited in the presence of function-blocking antibodies against both the alpha6 and beta1 integrin subunits and by the laminin E3 fragment...
  91. ncbi Immunological studies in recurrent spontaneous abortion: effects of immunization of women with paternal mononuclear cells on lymphocytotoxic and mixed lymphocyte reaction blocking antibodies and correlation with sharing of HLA and pregnancy outcome
    J B Smith
    Department of Medicine, Jefferson Medical College, Thomas Jefferson University, Philadelphia, PA 19107
    J Reprod Immunol 14:99-113. 1988
    ..Results so far suggest that conversion to LCTA positive after immunization may have a negative influence on pregnancy outcome...
  92. ncbi Clinical and laboratory aspects of thyroid autoantibodies
    David Sinclair
    Department of Clinical Biochemistry, Queen Alexandra Hospital, Portsmouth PO6 3LY, UK
    Ann Clin Biochem 43:173-83. 2006
    ..those antibodies recognizing the B subunit, located much nearer the cell surface, appear to function as blocking antibodies. Thyroid peroxidase (TPO), originally described as thyroid microsomal antigen, is present on the apical ..
  93. ncbi Analysis of costimulatory molecules OX40/4-1BB (CD134/CD137) detection on chosen mononuclear cells in children and adolescents with Graves' disease during methimazole therapy
    Artur Bossowski
    2nd Department of Children s Diseases, Medical University Bialystok, Poland
    J Pediatr Endocrinol Metab 18:1365-72. 2005
    ..Stimulating and blocking antibodies to the TSH-receptor using JPO9 CHO cells in unfractionated serum were measured by a highly sensitive ..
  94. pmc Structure-guided identification of a laminin binding site on the laminin receptor precursor
    Kelly V Jamieson
    Department of Pathology, New York University School of Medicine, New York, NY 10016, USA
    J Mol Biol 405:24-32. 2011
    ..In addition, the specific targeting of LamR with small interfering RNAs, blocking antibodies, and Sindbis viral vectors confers anti-tumor effects...
  95. ncbi Age-dependent effects of secreted Semaphorins 3A, 3F, and 3E on developing hippocampal axons: in vitro effects and phenotype of Semaphorin 3A (-/-) mice
    E Pozas
    Department of Cell Biology, University of Barcelona, E 08028, Spain
    Mol Cell Neurosci 18:26-43. 2001
    ..Such chemorepulsion is blocked by Neuropilin-1 and -2 blocking antibodies. However, at perinatal stages, the inner layers of the entorhinal cortex attract CA1 axons...
  96. ncbi Alpha1beta1-integrin is an essential signal for neurite outgrowth induced by thrombospondin type 1 repeats of SCO-spondin
    M Bamdad
    Faculte de Medecine, UMR INSERM 384 et Laboratoire de Biochimie médicale, 28 Place Henri Dunant, BP 38, 63001, Clermont Ferrand Cedex, France
    Cell Tissue Res 315:15-25. 2004
    ..In this study, we investigate whether function-blocking antibodies directed against integrin subunits can block these effects in cell line B104, cloned from a neuroblastoma ..
  97. pmc Integrin cross-talk in endothelial cells is regulated by protein kinase A and protein phosphatase 1
    Annette M Gonzalez
    Department of Cell and Molecular Biology, The Feinberg School of Medicine at Northwestern University, Chicago, Illinois 60611, USA
    J Biol Chem 283:31849-60. 2008
    In endothelial cells (ECs) beta1 integrin function-blocking antibodies inhibit alphavbeta3 integrin-mediated adhesion to a recombinant alpha4-laminin fragment (ralpha4LN fragment)...
  98. ncbi [Diagnosis of autoimmune thyroid disease]
    Bozo Trbojević
    Institute of Endocrinology, Diabetes and Metabolic Disorders, Clinical Centre of Serbia, Belgrade
    Srp Arh Celok Lek 133:25-33. 2005
    ..Patients with autoimmune thyroid disease may have both stimulating and blocking antibodies in their sera, the clinical picture being the result of the relative potency of each species; blocking ..
  99. ncbi Keratinocyte collagenase-1 expression requires an epidermal growth factor receptor autocrine mechanism
    B K Pilcher
    Division of Dermatology, Department of Medicine, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 274:10372-81. 1999
    ..that treatment of keratinocytes cultured on type I collagen with epidermal growth factor receptor (EGFR) blocking antibodies or a specific receptor antagonist inhibited cell migration across type I collagen and the matrix-directed ..
  100. pmc P-selectin glycoprotein ligand-1 mediates L-selectin-dependent leukocyte rolling in venules
    Markus Sperandio
    University of Virginia Health System, Cardiovascular Research Center, MR5 Bldg, Rm 1013, P O Box 801 394, Charlottesville, VA 22908 1394, USA
    J Exp Med 197:1355-63. 2003
    ..venules of inflamed tissues is reduced in L-selectin-deficient mice and mice treated with L-selectin blocking antibodies, but the glycoprotein ligand for L-selectin in inflamed venules is unknown...
  101. ncbi Role of selectins in the intestinal epithelial migration of eosinophils
    Sonia Michail
    Department of Pediatrics, Division of Gastroenterology and Nutrition and Wright State University School of Medicine, Dayton, OH 45404, USA
    Pediatr Res 58:644-7. 2005
    ..Subsequently, blocking antibodies toward L-selectin and PSGL-1 were used in a similar manner...

Research Grants75

  1. Cardiotrophin-Like Cytokine 1, a Candidate Molecule for the FSGS Factor
    Virginia J Savin; Fiscal Year: 2010
    ..05 to 50 ng/ml in established Palb and GEC monolayer protocols. Specificity of effects will be tested using blocking antibodies to CLA-1 and known blockers of FSGS plasma effects...
  2. Knockdown of Gene Expression in Immune Cells in Humanized Mice
    Judy Lieberman; Fiscal Year: 2010
    ..Experimental manipulations (i.e. gene knockout, blocking antibodies, and cytokines) that have been so powerful for dissecting the molecular basis of mouse immunology are ..
  3. Role of Platelet derived growth factor receptor-a in Liver Patho-biology
    SATDARSHAN SINGH MONGA; Fiscal Year: 2013
    ..in novel transgenic mice in our lab and complemented by utilization of species-specific PDGFR[unreadable] blocking antibodies to determine impact on disease progression in these models to address therapeutic efficacy...
  4. Tumor Cell Arrest and Adhesion in the Microcirculation
    Bingmei M Fu; Fiscal Year: 2013
    ..rates and a) under conditions of normal and increased permeability by VEGF, b) after pretreatment with the blocking antibodies to endothelial cell adhesion molecules, c) after pretreatment with the blocking antibodies to tumor cell ..
  5. Pulmonary Defenses Against Intracellular Infection
    Shawn J Skerrett; Fiscal Year: 2013
    ..This aim will use knockout mice and blocking antibodies, a model of airborne infection, and tissue culture studies to test the hypothesis that IL-1[unreadable] and ..
  6. DAMP-RAGE Signaling and Fetal Injury in Inflammation-Induced Preterm Birth
    Irina A Buhimschi; Fiscal Year: 2013
    ..We will test the efficacy of anti-HMGB1 and anti-RAGE blocking antibodies or potential therapeutic agents such as N-acetylcysteine and ethyl pyruvate to reverse the damaging effects ..
  7. Naturally Occurring IgM Anti-Leucocyte Autoantibodies Protect Against Renal IRI
    MARK DOUGLAS OKUSA; Fiscal Year: 2013
    ..We will interrupt this pathway in IgMko mice with blocking antibodies and depleting DCs with diptheria toxin...
  8. Role of Surfactant in Innate and Adaptive Immunity
    Jo Rae Wright; Fiscal Year: 2010
    ..b>Blocking antibodies and receptor null mice will be used to test the hypothesis that dendritic and mast cell specific responses ..
  9. Molecular Regulation of Vascular Regression
    Roberto Nicosia; Fiscal Year: 2013
    ..Functional studies will be performed with anti-INF-1 and -IFN-2 blocking antibodies. Experiments proposed in this grant will further our understanding of the cellular and molecular mechanisms ..
  10. Role of chemokines in the T cell response to ocular toxoplasmosis
    Christopher A Hunter; Fiscal Year: 2012
    ..In addition, administration of CXCL10-specific blocking antibodies leads to a decrease in T cell number in the eye and a corresponding increase in ocular pathology...
  11. Modulation of Glioma Growth and Invasion by HCMV Glycoprotein B
    Liliana Soroceanu; Fiscal Year: 2010
    ..glioma cell migration and that HCMV-induced glioma transwell migration is inhibited both by PDGFR1 blocking antibodies or gB neutralizing antibodies...
  12. Matrix and trophic coupling in the neurovascular unit
    Eng H Lo; Fiscal Year: 2011
    ..Integrin activating or blocking antibodies, ILK mutants and pharmacologic inhibitors are used to document these pathways...
  13. Cytokines and the blood-brain barrier in the ovine fetus
    Barbara S Stonestreet; Fiscal Year: 2012
    ..IL-6 and IL-1 2 blocking antibodies will be infused before ischemia...
  14. Recognition of Mtb-Infected Cells by Non-Classically Restritcted CD8+ T Cells
    David M Lewinsohn; Fiscal Year: 2013
    ..b>Blocking antibodies will be used to determine the relative contribution of each HLA-Ib allele...
  15. Regulatory Costimulatory Pathways &Fetomaternal Tolerance
    Indira Guleria; Fiscal Year: 2012
    ..vivo mechanisms involved in ICOS-B7h pathway induced fetomaternal tolerance utilizing ICOS and B7h specific blocking antibodies as well as gene deficient mice...
  16. Remote Inflammation and Atherothrombosis
    NICOLE LEANNE WARD; Fiscal Year: 2013
    ..mouse molecular genetics, therapeutic targeting strategies utilizing small molecule inhibitors and function blocking antibodies, coupled with bone marrow and skin transplant approaches we propose to identify pro-atherogenic and pro-..
  17. Specificity and mechanisms of lung lymphocyte homing
    LINH PHUONG NGUYEN; Fiscal Year: 2010
    ..Second, to determine the molecular players involved, we will use flow cytometry and blocking antibodies in in vivo migration assays to test the involvement of specifc endothelial and lymphocyte adhesion ..
  18. IgM Fc receptor in CLL
    Hiromi Kubagawa; Fiscal Year: 2013
    ..IgM antibodies for cross-linkage of the B-cell receptor and the Fc[unreadable]R along with Fc[unreadable]R -blocking antibodies, and is conceptually innovative as it is expected to provide the first demonstration of a survival function ..
  19. DEFINITION OF MICROENVIRONMENT IN BREAST CANCER
    Mina Bissell; Fiscal Year: 2013
    ..of Ln-1 signaling cascade components for mammary specific functions using inhibitory peptides, blocking antibodies, mutant cells and shRNA ablation in high throughput assays using Cellomics and other devices and designer ..
  20. FETAL AND POSTNATAL DEVELOPMENT OF VISUAL CONNECTIONS
    Carla J Shatz; Fiscal Year: 2013
    ..Direct blockade of PirB with recombinant soluble truncated PirB protein or function-blocking antibodies will also be used. These experiments should reveal when and in what cell types PirB acts...
  21. REGULATION OF SIGNALING BY CELL-SURFACE PROTEOLYSIS
    Nigel W Bunnett; Fiscal Year: 2010
    ..The role of PARs in colitis will be investigated using novel blocking antibodies, which circumvents the lack of small molecular antagonists...
  22. Cell-Matrix Interactions in Epithelial Polarization
    Karl S Matlin; Fiscal Year: 2010
    ..which integrin receptors for laminin are involved in the polarization process through the use of function-blocking antibodies, expression of mutant integrins, and RNA interference, and will map the signaling events leading from ..
  23. Renal Protection Through PD-1/PD-L1 Interactions
    Gilbert R Kinsey; Fiscal Year: 2013
    ..AKI in mouse models using both PD-1 knockout mice (compared to wild-type littermates) and PD-1 or PD-L1 blocking antibodies in wild-type mice...
  24. Mechanisms of social-stress enhanced allergic airway response in a mouse model
    Angela Haczku; Fiscal Year: 2013
    ..a recombinant mutant SP-D containing the carbohydrate recognition (CRD), but not the collagen domain, and blocking antibodies against the negative Signal-Regulatory Protein (SIRP) on antigen presenting and Th cell function, we will ..
  25. CLASSICAL AND ALTERNATIVE PATHWAYS OF FIBRINOLYSIS
    EDWARD FRANKLIN PLOW; Fiscal Year: 2013
    ..A panel of blocking antibodies to these five major Plg-Rs has been developed, have been shown to be specific for their target Plg-R, and ..
  26. CD80 and CD86 mediated innate immune response in sepsis
    Anna Nolan; Fiscal Year: 2013
    ..and septic human subjects on macrophages in vitro and assess the role CD80 and CD86 using using siRNA and blocking antibodies in co-culture experiments...
  27. Potent Phage T4 Derived V2 Immunogens as HIV Vaccines
    Venigalla B Rao; Fiscal Year: 2013
    ..for V2- [unreadable]4[unreadable]7 interfering antibodies, virus neutralizing antibodies and transmission-blocking antibodies. The best V2 variants down-selected from these assays will then be tested in the rabbit model...
  28. T Cell Homeostasis in Systemic Autoimmunity
    Argyrios N Theofilopoulos; Fiscal Year: 2012
    ..proliferation of transferred T cells into non- lymphopenic hosts, and inhibition of proliferation by IL-7R-blocking antibodies;b) define the mechanisms of cytokine receptor downregulation and their implications to autoimmunity by ..
  29. Integrin Mechanotransduction and caveolae-based signaling complexes
    VICTOR J RIZZO; Fiscal Year: 2012
    ..activity at the luminal surface and within purified caveolae in endothelial cells pretreated with integrin blocking antibodies. We propose that integrins expressed on the luminal endothelial cell surface translocate to caveolae ..
  30. CD4+ Memory T-Cells in Human Tumor Microenvironment
    Richard B Bankert; Fiscal Year: 2013
    ..This last aim is achieved using function blocking antibodies in a xenograft model in which tumor cells, T lymphocytes and fibroblasts are implanted into NOD-scid/IL-2 ..
  31. Elucidating the mechanisms of venous reprogramming during coronary development
    MARY K RED-HORSE; Fiscal Year: 2011
    ..role in vivo by assessing coronary vessel development in knockout animals or those treated with function-blocking antibodies or drugs. The initial part of the proposal will be carried out at Stanford University in Dr...
  32. Targeting IFN gamma and chemokines to treat vitiligo in a humanized mouse model
    John E Harris; Fiscal Year: 2013
    ..skills necessary to generate a humanized mouse model of vitiligo, test anti-human cytokine and chemokine blocking antibodies as novel treatments, and gain an understanding of clinical and translational research strategies for future ..
  33. Tumor-produced TNF alpha as an inducer of immune resistance and tumor survival
    ELENA LEVITSKAIA; Fiscal Year: 2013
    ..Disruption of TNF:TNF-R(s) interactions, using blocking antibodies or soluble TNF-R2, decreased human NB cell viability indicating that TNF constitutively generates a pro- ..
  34. Modeling PCa Bone Metastasis: Dual Role of E-selectin Ligands and Integrins
    STEVEN RUSSELL BARTHEL; Fiscal Year: 2012
    ..Control PCa cell lines, blocking antibodies, RNAi, small molecule antagonists and metabolic inhibitors will be employed to control for E-selectin ..
  35. B7H1 Mediated Immunosuppression in Glioma
    Andrew T Parsa; Fiscal Year: 2013
    ..expression of B7H1 provides another opportunity to reverse immunosuppression through administration of blocking antibodies against B7H1 or its receptor PD-1...
  36. A novel colonic crypt base secretory cell that supports colon stem cells
    MICHAEL EVAN ROTHENBERG; Fiscal Year: 2013
    ..It will make use of monoclonal cKit blocking antibodies, cytotoxins targeted to cKit+ cells, and small molecule cKit inhibitors...
  37. Hyaluronan and CD44 are Novel Targets for Acute Lung injury
    Patrick A Singleton; Fiscal Year: 2013
    ..strategies designed to further validate HA/CD44 regulation of vascular integrity (CD44v10-specific blocking antibodies, hyaluronidase and Rho Kinase inhibitors, siRNA) using ACE antibody-directed nanocarrier technologies to ..
  38. Elucidating the mechanisms of venous reprogramming during coronary development
    MARY K RED-HORSE; Fiscal Year: 2013
    ..role in vivo by assessing coronary vessel development in knockout animals or those treated with function-blocking antibodies or drugs. The initial part of the proposal will be carried out at Stanford University in Dr...
  39. Role of ICOS-B7h Regulatory Costimulatory Pathway in Fetomaternal Tolerance
    Indira Guleria; Fiscal Year: 2010
    ..vivo mechanisms involved in ICOS-B7h pathway induced fetomaternal tolerance utilizing ICOS and B7h specific blocking antibodies as well as gene deficient mice...
  40. Novel therapeutic antibodies for the treatment and prevention of AIDS
    BILL DON HARRIMAN; Fiscal Year: 2012
    ..For these reasons, a new strategy has emerged: to identify blocking antibodies against the HIV receptors or co-receptors, such as CCR5, rather than the virus itself...
  41. FGF Receptor in Myelin Function and Disease
    Rashmi Bansal; Fiscal Year: 2012
    ..Using FGFs that preferentially activate specific FGF-Rs and receptor-specific blocking antibodies, we test the hypothesis that during OL lineage progression, cells are differentially activated by ..
  42. Role of beta-1 containing integrins in JNK activation in lung mechanical stretch
    LINDY SARA KLAFF; Fiscal Year: 2010
    ..Then, by using blocking antibodies and/or RNA interference directed towards different integrin a subunits, I will further delineate the ..
  43. Molecular mechanisms of choroidal neovascularization and vascular homeostasis
    ALEXANDER GEORG MARNEROS; Fiscal Year: 2011
    ..perform experiments that use a combination of conditional gene targeting approaches and treatments with blocking antibodies in mice. We will test the role of VEGF, Flt1 and Flk1 for choroidal endothelial cell functions...
  44. Hematopoietic stem cell cytokine control of developmental vascularization
    George E Davis; Fiscal Year: 2013
    ..administration of c-Kit (the SCF receptor) and CXCR4 (the SDF-1[unreadable] receptor) inhibitors or blocking antibodies to SCF and IL-3 leads to vascular hemorrhage phenotypes in developing quail embryos that relates to defects ..
  45. Helper and suppressor roles of CD8+ T cells
    Julie Urban; Fiscal Year: 2009
    ..therapeutic settings. We will use small molecule inhibitors, blocking antibodies and transgenic mice in in vitro assays examining killing of DC by effector-stage CD8+ T cells and the ..
  46. Targeting HSP70 in autoimmune vitiligo
    ISABELLE CAROLINE LE POOLE; Fiscal Year: 2012
    ..of HSP70 will be defined in our newly established in vivo mouse model of autoimmune vitiligo, and [3] HSP70 blocking antibodies will be tested for functional activity and the ability to interfere with progressive vitiligo...
  47. The role of beta integrins in epidermal tumor progression
    Alexander Ungewickell; Fiscal Year: 2012
    ..We have designed experiments using blocking antibodies and RNA interference to target [unreadable]1 and [unreadable]4 integrin in a 3-D human epidermal tissue ..
  48. Mathematical Modeling of Matrix Metalloproteinase-9 Driven Left Ventricular Remod
    Yufang Jin; Fiscal Year: 2010
    ..This model will be validated by evaluating in vivo LV remodeling progress of MI mice treated with MMP-9 blocking antibodies at day 1 and day 3...
  49. Transport of Large Molecules and Viral Vectors in Tumors: Effects of Cytotoxic an
    Yves Boucher; Fiscal Year: 2013
    ..The tumor vasculature will be targeted with endothelial cell targeting agents (anti-integrin 14 or 15 blocking antibodies) or cytotoxics (cyclophosphamide, docetaxel) that target both endothelial and cancer cells...
  50. Targeting Plasmacytoid Dendritic Cells to Treat Human Myeloma
    Qing Yi; Fiscal Year: 2013
    ..will result in the development of novel combinational therapies, such as chemotherapy drugs plus BST2/ILT7 blocking antibodies, for the treatment of myeloma patients;they will also improve our understanding of the mechanisms and ..
  51. Etiology and Therapy of Rickets in the Hyp mouse model of XLH
    Marie Demay; Fiscal Year: 2013
    ..plate and on parathyroid function will be compared to that of direct inhibition of FGF23 action using blocking antibodies. The effects of these three treatments on bone will be evaluated histologically, histomorphometrically and ..
  52. Blocking immune evasion as a novel HSV vaccine approach
    HARVEY MICHAEL FRIEDMAN; Fiscal Year: 2010
    ..Immunizing to produce blocking antibodies that disrupt gC and gE functions may greatly improve the effectiveness of HSV vaccines.
  53. Characterization of Innate immune receptors
    Detlef Schuppan; Fiscal Year: 2009
    ..Identity and function of the receptor(s) will further be confirmed by use of function blocking antibodies, siRNA and signaling pathway inhibitors...
  54. Oral delivered bone building therapy for osteoporosis
    Debra L Ellies; Fiscal Year: 2013
    ..two decades of expertise on the role of Wnt in bone and her involvement in the development of sclerostin blocking antibodies has enriched her skills to now focus on developing small molecule sclerostin inhibitor therapeutics, as ..
  55. Mechanisms of Endotoxin-induced Breakdown of Tolerance in the Lung
    Timothy J Chapman; Fiscal Year: 2013
    ..We then will use blocking antibodies and genetically modified mouse strains to examine the importance of lung accumulation of Tregs to the ..
  56. Complement &T Cell Medicated Allograft Rejection
    PETER SCOTT HEEGER; Fiscal Year: 2013
    ..We will use knockout and transgenic mice along with pharmacological inhibitors, blocking antibodies and knockdown technologies to a) determine the in vivo effects of C3aR/C5aR signaling on Treg in ..
  57. Transmembrane Proteins Involved in Human Tumor Expansion
    James P Quigley; Fiscal Year: 2013
    ..and to use these cell pairs in new subtractive immunization approaches to generate novel function-blocking antibodies. Panels of mAbs will be screened for differential reactivity and function-blocking properties in our ..
  58. Factors Controlling Effector T Cell Maintenance in the Pathogenesis of Colitis
    Casey T Weaver; Fiscal Year: 2013
    ..These transgenic models are complemented by new blocking antibodies and recombinant proteins that we have acquired through collaboration...
  59. Malaria Vaccine Adjuvant Immunogenicity and Safety
    Nirbhay Kumar; Fiscal Year: 2013
    ..stage development of zygote into ookinete, represent well established target antigens of transmission-blocking antibodies. Antibodies recognizing specific conformational epitopes in these proteins are potent blockers of ..
  60. Role of hemeoxygenase-1 in experimental acute pancreatitis
    Aida Habtezion; Fiscal Year: 2013
    ..this aim, we propose to define trafficking molecule expression and then assess their functional role using blocking antibodies and/or mice genetically deficient in specified trafficking receptors...
  61. Complement &T Cell Medicated Allograft Rejection
    PETER SCOTT HEEGER; Fiscal Year: 2012
    ..We will use knockout and transgenic mice along with pharmacological inhibitors, blocking antibodies and knockdown technologies to a) determine the mechanisms linking C3aR/C5aR signaling with induction and ..
  62. Functional Imaging of Tumor-Associated Lymphatics
    Sunkuk Kwon; Fiscal Year: 2013
    ..in response to the anti-VEGF receptor (VEGFR)-3 blocking antibody or a combination of anti-VEGFR-2 and -3 blocking antibodies as a means to ameliorate the earliest tumor lymphangiogenesis and reduce the rate of metastasis in tumor-..
  63. Lymphangiogenesis and Angiogenesis in Airway Inflammation
    Donald McDonald; Fiscal Year: 2009
    ..The contribution of putative mediators and reversibility will be determined through the use of function-blocking antibodies, soluble decoy receptors, and receptor tyrosine kinase inhibitors...
  64. Falciparum malaria invasion ligand DNA/protein vaccine
    David Narum; Fiscal Year: 2002
    ..and purification, 2) biochemical, and functional characterization, and 3) study generation of EBP2 blocking antibodies and efficacy to inhibit parasite growth in vitro...
  65. XENOGENEIC MODEL FOR HOMING OF HUMAN LYMPHOCYTES
    Sharon Evans; Fiscal Year: 1993
    ..lymph node HEV of normal Balb/c and SCID mice and (b) examining the effect of L-selectin-specific blocking antibodies on the adhesion of human lymphocytes to murine HEV...
  66. SELECTIN MEDIATED LEUKOCYTE ADHESION IN VIVO
    Klaus Ley; Fiscal Year: 2002
    ..compare the shear resistance of P-selectin and E-selectin dependent rolling, (4) determine whether function-blocking antibodies to PSGL-1 (P-selectin Glycoprotein Ligand-1) can block P-selectin dependent rolling, and (5) compare the ..
  67. Craniofacial defects in ethanol-exposed zebrafish
    Sara Ahlgren; Fiscal Year: 2004
    ..Partial inhibition of Sonic hedgehog in the chick embryo using function-blocking antibodies results in a phenotype similar to a mild holoprosencephaly that is intriguingly similar to fetal alcohol ..
  68. BINDING OF ALLERGENS TO LIPOSOMES FOR IMMUNOTHERAPY
    ARISTO WOJDANI; Fiscal Year: 1990
    ..or without BRMs will correct the faulty regulation in T cell function and may lead to production of IgG blocking antibodies but not IgE. To test this hypothesis, in Phase I it is planned to: 1...
  69. ROLE OF 02 RADICALS AND IRON IN ASBESTOS-INDUCED CANCER
    ANN AUST; Fiscal Year: 2003
    ..Whether the alpha v beta 6 integrin is involved in the activation will be determined using blocking antibodies specific for this integrin...
  70. INTRACELLULAR INFECTION OF HUMAN ALVEOLAR MACROPHAGES
    David Park; Fiscal Year: 2001
    ..cytokines on intracellular growth will be measured using selected combinations of recombinant cytokines and blocking antibodies. Specific interactions signaling cytokine induction by live bacteria and cell wall glycolipids will be ..
  71. RAGE and a Novel Tumor Axis
    Ann Schmidt; Fiscal Year: 2005
    ..interaction mediates outgrowth of cerebral cortical neurites, as the process is inhibited by blocking antibodies to RAGE, or soluble RAGE (sRAGE), the extracellular ligand-binding domain of RAGE...
  72. Matrix Protein in Human Epidermal Neoplasia
    Robin Kimmel; Fiscal Year: 2006
    ..Laminin 5 blocking antibodies will be tested for their ability to inhibit growth of established Ras-induced tumors...
  73. INTEGRIN REGULATION AND FUNCTIONS IN ADULT BRAIN
    Christine Gall; Fiscal Year: 2007
    ..synaptic responses in electrophysiological studies of acute hippocampal slices; integrin function blocking antibodies will be used to identify integrins mediating these effects and to test if integrin effects are tonic or ..
  74. RAGE and modulation of tumor properties
    Emina Huang; Fiscal Year: 2006
    ..interaction mediated outgrowth of cerebral cortical neurites, as the process is inhibited by blocking antibodies to RAGE, or soluble RAGE (sRAGE), the extracellular ligand-binding domain of RAGE...
  75. Cyclic Follicle Function and Angiogenesis
    Ralf Zimmermann; Fiscal Year: 2006
    ..define the role of VEGF and its receptors VEGF-R1 and VEGF-R2 in cyclic folliculogenesis using specific blocking antibodies and ad-vectors expressing angiogenic substances...