antibodies

Summary

Summary: Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).

Top Publications

  1. ncbi A beta peptide vaccination prevents memory loss in an animal model of Alzheimer's disease
    D Morgan
    Department of Pharmacology, University of South Florida, Tampa 33612, USA
    Nature 408:982-5. 2000
  2. doi Nanobodies: natural single-domain antibodies
    Serge Muyldermans
    Research group Cellular and Molecular Immunology, Vrije Universiteit Brussel, 1050 Brussels, Belgium
    Annu Rev Biochem 82:775-97. 2013
  3. ncbi Aptamers: an emerging class of molecules that rival antibodies in diagnostics
    S D Jayasena
    NeXstar Pharmaceuticals, Inc, 2860 Wilderness Place, Boulder, CO 80301, USA
    Clin Chem 45:1628-50. 1999
  4. doi The toxicity of antiprion antibodies is mediated by the flexible tail of the prion protein
    Tiziana Sonati
    Institute of Neuropathology, University Hospital Zurich, Schmelzbergstrasse 12, CH 8091 Zurich, Switzerland
    Nature 501:102-6. 2013
  5. pmc α-synuclein reactive antibodies as diagnostic biomarkers in blood sera of Parkinson's disease patients
    Kiran Yanamandra
    Department of Medical Biochemistry and Biophysics, Umea University, Umea, Sweden
    PLoS ONE 6:e18513. 2011
  6. doi A genecentric Human Protein Atlas for expression profiles based on antibodies
    Lisa Berglund
    Department of Proteomics, School of Biotechnology, AlbaNova University Center, Royal Institute of Technology, Stockholm, Sweden
    Mol Cell Proteomics 7:2019-27. 2008
  7. pmc Clinical experience and laboratory investigations in patients with anti-NMDAR encephalitis
    Josep Dalmau
    Department of Neurology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
    Lancet Neurol 10:63-74. 2011
  8. doi Radiotherapy increases the permissiveness of established mammary tumors to rejection by immunomodulatory antibodies
    Inge Verbrugge
    Cancer Therapeutics Program, Sir Donald and Lady Trescowthick Laboratories, Department of Physical Sciences, Peter MacCallum Cancer Centre, East Melbourne, Vic, Australia
    Cancer Res 72:3163-74. 2012
  9. pmc Structural classification of toxic amyloid oligomers
    Charles G Glabe
    Department of Molecular Biology and Biochemistry, University of California, Irvine, California 92697, USA
    J Biol Chem 283:29639-43. 2008
  10. ncbi Subacute meningoencephalitis in a subset of patients with AD after Abeta42 immunization
    J M Orgogozo
    Federation of Neurology, CHU Pellegrin, Bordeaux, France
    Neurology 61:46-54. 2003

Research Grants

  1. Discovery metabolite profiling of the prolyl peptidases
    Alan Saghatelian; Fiscal Year: 2009
  2. Personalized Clinical Diagnostics and Beyond: Integrated Ring Resonator Arrays
    Ryan Bailey; Fiscal Year: 2009
  3. CFI Pathogen Inactivation Technology
    Trevor P Castor; Fiscal Year: 2010
  4. Ian C Michelow; Fiscal Year: 2016
  5. ASTHMA AND ALLERGIC DISEASE CENTER
    Thomas A Platts Mills; Fiscal Year: 2010
  6. Cardiotrophin-Like Cytokine 1, a Candidate Molecule for the FSGS Factor
    Virginia J Savin; Fiscal Year: 2010
  7. Cross-talk Between Coagulation, Inflammation, and Immunity
    Lawrence L Leung; Fiscal Year: 2013
  8. Importance of antigen specific immunoglobulin responses in controlling SIV/SHIV i
    Bapi Pahar; Fiscal Year: 2010
  9. Emilio B Gonzalez; Fiscal Year: 2014
  10. Optimized Growth Media to Detect Intracellular Bacteria Implicated in Chronic Dis
    PATRICIA MASCARELLI; Fiscal Year: 2011

Detail Information

Publications472 found, 100 shown here

  1. ncbi A beta peptide vaccination prevents memory loss in an animal model of Alzheimer's disease
    D Morgan
    Department of Pharmacology, University of South Florida, Tampa 33612, USA
    Nature 408:982-5. 2000
    ..The A beta-vaccinated mice also had a partial reduction in amyloid burden at the end of the study. This therapeutic approach may thus prevent and, possibly, treat Alzheimer's dementia...
  2. doi Nanobodies: natural single-domain antibodies
    Serge Muyldermans
    Research group Cellular and Molecular Immunology, Vrije Universiteit Brussel, 1050 Brussels, Belgium
    Annu Rev Biochem 82:775-97. 2013
    Sera of camelids contain both conventional heterotetrameric antibodies and unique functional heavy (H)-chain antibodies (HCAbs)...
  3. ncbi Aptamers: an emerging class of molecules that rival antibodies in diagnostics
    S D Jayasena
    NeXstar Pharmaceuticals, Inc, 2860 Wilderness Place, Boulder, CO 80301, USA
    Clin Chem 45:1628-50. 1999
    b>Antibodies, the most popular class of molecules providing molecular recognition needs for a wide range of applications, have been around for more than three decades...
  4. doi The toxicity of antiprion antibodies is mediated by the flexible tail of the prion protein
    Tiziana Sonati
    Institute of Neuropathology, University Hospital Zurich, Schmelzbergstrasse 12, CH 8091 Zurich, Switzerland
    Nature 501:102-6. 2013
    ..Ligands included seven distinct monoclonal antibodies, monovalent Fab1 fragments and recombinant single-chain variable fragment miniantibodies...
  5. pmc α-synuclein reactive antibodies as diagnostic biomarkers in blood sera of Parkinson's disease patients
    Kiran Yanamandra
    Department of Medical Biochemistry and Biophysics, Umea University, Umea, Sweden
    PLoS ONE 6:e18513. 2011
    Auto-antibodies with specificity to self-antigens have been implicated in a wide variety of neurological diseases, including Parkinson's (PD) and Alzheimer's diseases, being sensitive indicators of neurodegeneration and focus for disease ..
  6. doi A genecentric Human Protein Atlas for expression profiles based on antibodies
    Lisa Berglund
    Department of Proteomics, School of Biotechnology, AlbaNova University Center, Royal Institute of Technology, Stockholm, Sweden
    Mol Cell Proteomics 7:2019-27. 2008
    ..Using antibodies, it might be possible to design protein-specific probes for a representative protein from every protein-coding ..
  7. pmc Clinical experience and laboratory investigations in patients with anti-NMDAR encephalitis
    Josep Dalmau
    Department of Neurology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
    Lancet Neurol 10:63-74. 2011
    Since its discovery in 2007, the encephalitis associated with antibodies against the N-methyl-D-aspartate receptor (NMDAR) has entered the mainstream of neurology and other disciplines...
  8. doi Radiotherapy increases the permissiveness of established mammary tumors to rejection by immunomodulatory antibodies
    Inge Verbrugge
    Cancer Therapeutics Program, Sir Donald and Lady Trescowthick Laboratories, Department of Physical Sciences, Peter MacCallum Cancer Centre, East Melbourne, Vic, Australia
    Cancer Res 72:3163-74. 2012
    ..established triple-negative breast tumors could be enhanced with combinations of clinically relevant monoclonal antibodies (mAb), designed to stimulate immunity [anti-(α)-CD137, α-CD40] or relieve immunosuppression [α-programmed ..
  9. pmc Structural classification of toxic amyloid oligomers
    Charles G Glabe
    Department of Molecular Biology and Biochemistry, University of California, Irvine, California 92697, USA
    J Biol Chem 283:29639-43. 2008
    ..In view of the difficulty in determining the precise structure of amyloids, conformation- and epitope-specific antibodies may provide a facile means of classifying amyloid oligomer structures...
  10. ncbi Subacute meningoencephalitis in a subset of patients with AD after Abeta42 immunization
    J M Orgogozo
    Federation of Neurology, CHU Pellegrin, Bordeaux, France
    Neurology 61:46-54. 2003
    ..A Phase I study in AD patients demonstrated good safety and tolerability of multiple injections of aggregated Abeta42 (AN1792) with QS-21 as adjuvant...
  11. ncbi Selenium supplementation in patients with autoimmune thyroiditis decreases thyroid peroxidase antibodies concentrations
    Roland Gärtner
    Department of Endocrinology, Medizinische Klinik Innenstadt, University of Munich, D 80336 Munich, Germany
    J Clin Endocrinol Metab 87:1687-91. 2002
    ..5 +/- 0.7 yr) with autoimmune thyroiditis and thyroid peroxidase antibodies (TPOAb) and/or Tg antibodies (TgAb) above 350 IU/ml...
  12. doi Frequency and prognostic impact of antibodies to aquaporin-4 in patients with optic neuritis
    Sven Jarius
    Neurosciences Group, Weatherall Institute of Molecular Medicine, University of Oxford, Oxford, UK
    J Neurol Sci 298:158-62. 2010
    b>Antibodies to aquaporin-4 (AQP4-Ab) are found in 60-80% of patients with neuromyelitis optica (NMO), a severely disabling inflammatory CNS disorder of putative autoimmune aetiology, which predominantly affects the optic nerves and spinal ..
  13. ncbi Phage antibodies: filamentous phage displaying antibody variable domains
    J McCafferty
    MRC Laboratory of Molecular Biology, Cambridge, UK
    Nature 348:552-4. 1990
    New ways of making antibodies have recently been demonstrated using gene technology. Immunoglobulin variable (V) genes are amplified from hybridomas or B cells using the polymerase chain reaction, and cloned into expression vectors...
  14. doi Ubiquitin chain editing revealed by polyubiquitin linkage-specific antibodies
    Kim Newton
    Department of Physiological Chemistry, Genentech, Inc, 1 DNA Way, South San Francisco, CA 94080, USA
    Cell 134:668-78. 2008
    ..We have developed linkage-specific antibodies that recognize polyubiquitin chains joined through lysine 63 (K63) or 48 (K48)...
  15. doi Inhibition of hepatitis C virus infection by anti-claudin-1 antibodies is mediated by neutralization of E2-CD81-claudin-1 associations
    Sophie E Krieger
    Institut National de la Sante et de la Recherche Medicale, U748, Strasbourg, France
    Hepatology 51:1144-57. 2010
    ..Due to the lack of neutralizing anti-CLDN1 antibodies, the role of CLDN1 in the viral entry process is poorly understood...
  16. ncbi Common structure of soluble amyloid oligomers implies common mechanism of pathogenesis
    Rakez Kayed
    Department of Molecular Biology and Biochemistry, University of California, Irvine, CA 92697 3900, USA
    Science 300:486-9. 2003
    ..These results indicate that different types of soluble amyloid oligomers have a common structure and suggest they share a common mechanism of toxicity...
  17. pmc The nuclear factor HMGB1 mediates hepatic injury after murine liver ischemia-reperfusion
    Allan Tsung
    Department of Surgery, University of Pittsburgh, Pittsburgh, PA 15213, USA
    J Exp Med 201:1135-43. 2005
    ..Together, these results demonstrate that HMGB1 is an early mediator of injury and inflammation in liver I/R and implicates TLR4 as one of the receptors that is involved in the process...
  18. pmc B cell-helper neutrophils stimulate the diversification and production of immunoglobulin in the marginal zone of the spleen
    Irene Puga
    Institut Municipal d Investigació Mèdica Hospital del Mar, Barcelona, Spain
    Nat Immunol 13:170-80. 2011
    ....
  19. pmc Preexisting donor-specific HLA antibodies predict outcome in kidney transplantation
    Carmen Lefaucheur
    Département de Néphrologie et Transplantation Rénale, Hopital Saint Louis, Paris, France
    J Am Soc Nephrol 21:1398-406. 2010
    The clinical importance of preexisting HLA antibodies at the time of transplantation, identified by contemporary techniques, is not well understood...
  20. pmc The intracellular virus-containing compartments in primary human macrophages are largely inaccessible to antibodies and small molecules
    Hin Chu
    Department of Pediatrics, Emory University and Children s Healthcare of Atlanta, Atlanta, Georgia, United States of America
    PLoS ONE 7:e35297. 2012
    ..designed to determine if the VCC in macrophages was open to the external environment and accessible to antibodies and small molecules...
  21. pmc Limited efficacy of inactivated influenza vaccine in elderly individuals is associated with decreased production of vaccine-specific antibodies
    Sanae Sasaki
    Department of Immunology and Microbiology, Stanford University School of Medicine, Stanford, California, USA
    J Clin Invest 121:3109-19. 2011
    ..Here, we show that this is due to reduced quantities of vaccine-specific antibodies, rather than a lack of antibody avidity or affinity...
  22. pmc Antibodies to merkel cell polyomavirus T antigen oncoproteins reflect tumor burden in merkel cell carcinoma patients
    Kelly G Paulson
    Division of Human Biology, Fred Hutchinson Cancer Research Center, University of Washington, Seattle, Washington 98109, USA
    Cancer Res 70:8388-97. 2010
    ..Serum antibodies recognizing the MCPyV capsid protein VP1 are detectable at high titer in nearly all MCC patients and remain ..
  23. ncbi Rapid generation of functional human IgG antibodies derived from Fab-on-phage display libraries
    Thomas Jostock
    Dyax s a, Sart Tilman, Liege, Belgium
    J Immunol Methods 289:65-80. 2004
    We introduce a procedure for the rapid generation of fully human antibodies derived from "Fab-on-phage" display libraries...
  24. ncbi Clinical effects of Abeta immunization (AN1792) in patients with AD in an interrupted trial
    S Gilman
    Department of Neurology, University of Michigan, 300 N Ingalls 3D15, Ann Arbor, MI 48109 0489, USA
    Neurology 64:1553-62. 2005
    ..The authors report the results of a phase IIa immunotherapy trial of AN1792(QS-21) in patients with mild to moderate Alzheimer disease (AD) that was interrupted because of meningoencephalitis in 6% of immunized patients...
  25. pmc Single domain antibodies: promising experimental and therapeutic tools in infection and immunity
    Janusz Wesolowski
    Institute of Immunology, University Medical Center Hamburg Eppendorf, Hamburg, Germany
    Med Microbiol Immunol 198:157-74. 2009
    b>Antibodies are important tools for experimental research and medical applications. Most antibodies are composed of two heavy and two light chains. Both chains contribute to the antigen-binding site which is usually flat or concave...
  26. ncbi Interaction of Tim-3 and Tim-3 ligand regulates T helper type 1 responses and induction of peripheral tolerance
    Catherine A Sabatos
    Department of Neurology, Center for Neurologic Diseases, Brigham and Women s Hospital and Harvard Medical School, Boston, Massachusetts 02115, USA
    Nat Immunol 4:1102-10. 2003
    ..These data indicate that interaction of Tim-3 with Tim-3 ligand may serve to inhibit effector T(H)1 cells during a normal immune response and may be crucial for the induction of peripheral tolerance...
  27. ncbi Fine epitope mapping of anti-epidermal growth factor receptor antibodies through random mutagenesis and yeast surface display
    Ginger Chao
    Department of Chemical Engineering, Massachusetts Institute of Technology, MIT 66 552, Cambridge, MA 02139, USA
    J Mol Biol 342:539-50. 2004
    Fine epitope mapping of therapeutically relevant monoclonal antibodies (mAbs) specific for the epidermal growth factor receptor (EGFR) was accomplished through random mutagenesis and yeast surface display...
  28. pmc Unique carbohydrate-carbohydrate interactions are required for high affinity binding between FcgammaRIII and antibodies lacking core fucose
    Claudia Ferrara
    Pharma Research and Early Development, Roche Glycart AG, CH 8952 Schlieren, Switzerland
    Proc Natl Acad Sci U S A 108:12669-74. 2011
    ..b>Antibodies lacking core fucosylation show a large increase in affinity for FcγRIIIa leading to an improved receptor-..
  29. ncbi The receptor DEC-205 expressed by dendritic cells and thymic epithelial cells is involved in antigen processing
    W Jiang
    Laboratory of Molecular Immunology, Rockefeller University, New York, New York 10021, USA
    Nature 375:151-5. 1995
    ..Rabbit antibodies that bind DEC-205 are presented to reactive T-cell hybridomas 100-fold more efficiently than rabbit antibodies ..
  30. ncbi Probiotics and antibodies to TNF inhibit inflammatory activity and improve nonalcoholic fatty liver disease
    Zhiping Li
    The Johns Hopkins University, Baltimore, MD 21205, USA
    Hepatology 37:343-50. 2003
    ..NAFLD may have a similar pathogenesis, treatment with a probiotic (to modify the intestinal flora) or anti-TNF antibodies (to inhibit TNF-alpha activity) may improve NAFLD in ob/ob mice...
  31. ncbi Suppression of non-specific binding in serological Luminex assays
    Tim Waterboer
    Infection and Cancer Program, German Cancer Research Center DKFZ, Im Neuenheimer Feld 242, 69120 Heidelberg, Germany
    J Immunol Methods 309:200-4. 2006
    Bead-based suspension array technology (xMAP, Luminex Corp.) permits the simultaneous analysis of antibodies with specificities for up to 100 different antigens in a single reaction and the high through-put screening of up to 1,000 sera ..
  32. pmc Novel antibodies reveal inclusions containing non-native SOD1 in sporadic ALS patients
    Karin Forsberg
    Department of Medical Biosciences, Umea University, Umea, Sweden
    PLoS ONE 5:e11552. 2010
    ..Non-native and aggregation-prone forms of mutant SOD1s are thought to trigger the disease. Two sets of novel antibodies, raised in rabbits and chicken, against peptides spaced along the human SOD1 sequence, were by enzyme-linked ..
  33. ncbi Maintenance of serological memory by polyclonal activation of human memory B cells
    Nadia L Bernasconi
    Institute for Research in Biomedicine, Via Vela 6, CH 6500 Bellinzona, Switzerland
    Science 298:2199-202. 2002
    Production of antibodies can last for a lifetime, through mechanisms that remain poorly understood...
  34. ncbi In vivo bioactivity of interferon-beta in multiple sclerosis patients with neutralising antibodies is titre-dependent
    A Sominanda
    Karolinska Institutet, Department of Clinical Neuroscience, Division of Neurology R54, Karolinska University Hospital Huddinge, SE 141 86 Stockholm, Sweden
    J Neurol Neurosurg Psychiatry 79:57-62. 2008
    Development of neutralising antibodies (NAbs) against recombinant interferon-beta (IFNbeta) is a significant clinical problem in the treatment of multiple sclerosis (MS)...
  35. pmc Clinical protection from falciparum malaria correlates with neutrophil respiratory bursts induced by merozoites opsonized with human serum antibodies
    Charlotte Joos
    Institut Pasteur de Dakar, Unite d immunologie, Dakar, Senegal
    PLoS ONE 5:e9871. 2010
    ..Repeated blood stage infections induce antibodies considered to be the main arbiters of protection from pathology, but their essential functions have remained ..
  36. doi Serological antibodies in inflammatory bowel disease: a systematic review
    Lani Prideaux
    Department of Gastroenterology, St Vincent s Hospital Melbourne, Australia
    Inflamm Bowel Dis 18:1340-55. 2012
    ..This systematic review focuses on the potential for 10 serological antibodies to fill these roles: pANCA, ASCA, anti-OmpC, anti-CBir1, anti-I2, ALCA, ACCA, AMCA, anti-L, and anti-C...
  37. pmc Long-term effect of antibodies against infused alpha-galactosidase A in Fabry disease on plasma and urinary (lyso)Gb3 reduction and treatment outcome
    Saskia M Rombach
    Department of Endocrinology and Metabolism, Division of Internal Medicine, Academic Medical Center, Amsterdam, The Netherlands
    PLoS ONE 7:e47805. 2012
    ..Anti agalsidase ABs negatively influence globotriaosylceramide (Gb3) reduction. We investigated the impact of agalsidase AB on Gb3 and lysoGb3 and clinical outcome in Fabry patients on ERT...
  38. ncbi Clinical development of plant-produced recombinant pharmaceuticals: vaccines, antibodies and beyond
    Vidadi Yusibov
    Fraunhofer USA Center for Molecular Biotechnology, Newark, DE, USA
    Hum Vaccin 7:313-21. 2011
    ..cell culture, a variety of recombinant subunit vaccine candidates, therapeutic proteins, including monoclonal antibodies, and dietary proteins have been produced...
  39. ncbi Detection of species-specific antibody response of humans and mice bitten by sand flies
    I Rohousova
    Department of Parasitology, Faculty of Science, Charles University, Vinicna 7, 128 44 Prague 2, Czech Republic
    Parasitology 130:493-9. 2005
    ..Our data suggest that the antibody response to sand fly saliva could be used for monitoring the exposure of humans and other hosts to sand flies and might be used as a marker of risks for Leishmania transmission in endemic areas...
  40. doi Antibody-based proteomics: fast-tracking molecular diagnostics in oncology
    Donal J Brennan
    UCD School of Biomolecular and Biomedical Science, UCD Conway Institute, University College Dublin, Belfield, Dublin 4, Ireland
    Nat Rev Cancer 10:605-17. 2010
    ..Although the clinical utility of these emerging technologies remains to be established, the traditional use of antibodies as affinity reagents in clinical diagnostic and predictive assays suggests that the rapid translation of such ..
  41. doi Clinical significance of anti-Ro52 (TRIM21) antibodies non-associated with anti-SSA 60kDa antibodies: results of a multicentric study
    P Ghillani
    Laboratoire d Immunochimie, Groupe Hospitalier Pitie Salpetriere, Paris, France
    Autoimmun Rev 10:509-13. 2011
    Ro52 antigen has recently been identified as TRIM21 protein, but the clinical significance of anti-Ro52/TRIM21 antibodies remains controversial...
  42. ncbi Development of antiinfliximab antibodies and relationship to clinical response in patients with rheumatoid arthritis
    Gerrit Jan Wolbink
    Department of Immunopathology, CLB Sanquin Amsterdam and Jan van Breeman Institute, Amsterdam, The Netherlands
    Arthritis Rheum 54:711-5. 2006
    ..monoclonal IgG1 antibody against tumor necrosis factor, may result in the formation of infliximab-specific IgG antibodies. This study evaluated the clinical significance of these antibodies in patients with rheumatoid arthritis (RA).
  43. pmc Camelid single domain antibodies (VHHs) as neuronal cell intrabody binding agents and inhibitors of Clostridium botulinum neurotoxin (BoNT) proteases
    Jacqueline M Tremblay
    Tufts Cummings School of Veterinary Medicine, Department of Biomedical Sciences, 200 Westboro Road, North Grafton, MA 01536, USA
    Toxicon 56:990-8. 2010
    ..These results demonstrate that VHH domains have potential as components of therapeutic agents for reversal of botulism intoxication...
  44. ncbi Biochemical and histochemical evidence of nonspecific binding of alpha7nAChR antibodies to mouse brain tissue
    Donna L Herber
    Department of Pharmacology and Therapeutics, University of South Florida, 12901 Bruce B Downs Blvd, MDC Box 9, Tampa, FL 33612 4799, USA
    J Histochem Cytochem 52:1367-76. 2004
    ..protocols for immunohistochemistry and Western blotting were followed using several commercially available antibodies. Various mice were evaluated, including non-transgenics, APP, PS1, APP+PS1, and alpha7 knockouts...
  45. pmc Paraneoplastic anti-N-methyl-D-aspartate receptor encephalitis associated with ovarian teratoma
    Josep Dalmau
    Department of Neurology, Division of Neuro Oncology, University of Pennsylvania, Philadelphia, PA 19104, USA
    Ann Neurol 61:25-36. 2007
    ..To report the autoantigens of a new category of treatment-responsive paraneoplastic encephalitis...
  46. pmc Induction of epitope-specific neutralizing antibodies against West Nile virus
    Theodore Oliphant
    Departments of Medicine, Molecular Microbiology, and Pathology and Immunology, Washington University School of Medicine, 660 South Euclid Avenue, St Louis, MO 63110, USA
    J Virol 81:11828-39. 2007
    ..III (DIII-lr) of West Nile virus (WNV) envelope (E) protein is recognized by strongly neutralizing type-specific antibodies. In contrast, an epitope against the fusion loop in domain II (DII-fl) is recognized by flavivirus cross-..
  47. doi Treatment with monoclonal antibodies against Clostridium difficile toxins
    Israel Lowy
    Medarex, Princeton, NJ, USA
    N Engl J Med 362:197-205. 2010
    ..New therapies are needed to manage the increasing incidence, severity, and high rate of recurrence of Clostridium difficile infection...
  48. ncbi Targeting and tracing antigens in live cells with fluorescent nanobodies
    Ulrich Rothbauer
    Ludwig Maximilians University Munich, Department of Biology II, Grosshaderner Str 2, 82152 Planegg Martinsried, Germany
    Nat Methods 3:887-9. 2006
    We fused the epitope-recognizing fragment of heavy-chain antibodies from Camelidae sp. with fluorescent proteins to generate fluorescent, antigen-binding nanobodies (chromobodies) that can be expressed in living cells...
  49. doi Banff '09 meeting report: antibody mediated graft deterioration and implementation of Banff working groups
    B Sis
    Department of Laboratory Medicine and Pathology, University of Alberta, Edmonton, Alberta, Canada
    Am J Transplant 10:464-71. 2010
    ....
  50. pmc Discovery of markers of exposure specific to bites of Lutzomyia longipalpis, the vector of Leishmania infantum chagasi in Latin America
    Clarissa Teixeira
    Vector Molecular Biology Section, Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, Maryland, United States of America
    PLoS Negl Trop Dis 4:e638. 2010
    ..Though some proteins are immunogenic and have potential as markers of vector exposure, their identity and vector specificity remain elusive...
  51. doi Advances in the assessment and control of the effector functions of therapeutic antibodies
    Xu Rong Jiang
    Analytical Biochemistry, MedImmune, Gaithersburg, Maryland 20878, USA
    Nat Rev Drug Discov 10:101-11. 2011
    The Fc (crystallizable fragment) region of therapeutic antibodies can have an important role in their safety and efficacy...
  52. pmc Encephalitis and antibodies to synaptic and neuronal cell surface proteins
    Eric Lancaster
    Department of Neurology, University of Pennsylvania, Philadelphia, PA 19104, USA
    Neurology 77:179-89. 2011
    The identification of encephalitis associated with antibodies against cell surface and synaptic proteins, although recent, has already had a substantial impact in clinical neurology and neuroscience...
  53. ncbi Natural human humoral response to salivary gland proteins of Anopheles mosquitoes in Thailand
    Amornrat Waitayakul
    Department of Parasitology, Faculty of Medical Technology, Mahidol University, Salaya, Nakhonpathom, Thailand
    Acta Trop 98:66-73. 2006
    ..Here we have demonstrated that anti-Anopheles salivary protein antibodies occurred predominantly in patients with acute Plasmodium falciparum or P...
  54. pmc Llama antibody fragments with cross-subtype human immunodeficiency virus type 1 (HIV-1)-neutralizing properties and high affinity for HIV-1 gp120
    Anna Forsman
    MRC UCL Centre for Medical Molecular Virology, Division of Infection and Immunity, University College London, 46 Cleveland Street, London W1T 4JF, United Kingdom
    J Virol 82:12069-81. 2008
    ..We have characterized variable domains of these heavy chain antibodies, the VHH, from llamas immunized with human immunodeficiency virus type 1 (HIV-1) envelope protein gp120 in order ..
  55. pmc Human antibodies reveal a protective epitope that is highly conserved among human and nonhuman influenza A viruses
    Andres G Grandea
    Theraclone Sciences, Seattle, WA 98104, USA
    Proc Natl Acad Sci U S A 107:12658-63. 2010
    ..Monoclonal antibodies that target highly conserved viral epitopes might offer an alternative protection paradigm...
  56. pmc Analyzing the validity of GalR1 and GalR2 antibodies using knockout mice
    Xiaoying Lu
    The Harold L Dorris Neurological Research Institute, Molecular and Integrative Neurosciences Department, The Scripps Research Institute, SR 307, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Naunyn Schmiedebergs Arch Pharmacol 379:417-20. 2009
    ..The lack of sensitive and selective antibodies capable of recognizing endogenous GPCRs, however, hampers the progress of research on this class of receptors...
  57. doi Human milk-derived B cells: a highly activated switched memory cell population primed to secrete antibodies
    Edouard Tuaillon
    Unité de Recherche EA 4205, Universite Montpellier 1, and Laboratoire de Virologie, Centre Hospitalier Universitaire de Montpellier, Montpellier, France
    J Immunol 182:7155-62. 2009
    ..Breast milk B cells display a phenotype strikingly different from blood, are primed to secrete Abs, and have a mucosal homing profile similar to B cells located in gut-associated lymphoid tissue...
  58. ncbi Phosphoprotein analysis using antibodies broadly reactive against phosphorylated motifs
    Hui Zhang
    Cell Signaling Technology, Beverly, Massachusetts 01915, USA
    J Biol Chem 277:39379-87. 2002
    ..to any protein kinase for which a substrate consensus motif has been identified, we illustrate here the use of antibodies broadly reactive against phosphorylated Ser/Thr-motifs typical of AGC kinase substrates...
  59. ncbi Characterization of protein kinase A and protein kinase C phosphorylation of the N-methyl-D-aspartate receptor NR1 subunit using phosphorylation site-specific antibodies
    W G Tingley
    Department of Neuroscience, Howard Hughes Medical Institute, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    J Biol Chem 272:5157-66. 1997
    ..of the NR1 subunit of N-methyl-D-aspartate receptors in situ, we have generated several polyclonal antibodies that recognize the NR1 subunit only when specific serine residues are phosphorylated...
  60. ncbi Isolating and engineering human antibodies using yeast surface display
    Ginger Chao
    Department of Chemical Engineering and Biological Engineering Division, Massachusetts Institute of Technology, 77 Massachusetts Avenue E19 563, Cambridge, Massachusetts 02139, USA
    Nat Protoc 1:755-68. 2006
    This protocol describes the process of isolating and engineering antibodies or proteins for increased affinity and stability using yeast surface display...
  61. doi Antibodies and neuronal autoimmune disorders of the CNS
    Francesc Graus
    Servei de Neurologia, Hospital Clinic, Institut d Investigacio Biomedica August Pi i Sunyer, Villarroel 170, 08036, Barcelona, Spain
    J Neurol 257:509-17. 2010
    We review the neuronal antibodies described in CNS disorders in order to clarify their diagnostic value, emphasize potentials pitfalls and limitations in the diagnosis of paraneoplastic neurological syndromes (PNS), and examine the ..
  62. ncbi Recommendations for the design and optimization of immunoassays used in the detection of host antibodies against biotechnology products
    Anthony R Mire-Sluis
    Office of Biotechnology Products, Center for Drug Evaluation and Research, FDA, Rockville, MD 20852, USA
    J Immunol Methods 289:1-16. 2004
    ..Sections on assay performance parameters, including those that appear in regulatory guidances, are contained in this manuscript...
  63. pmc Addiction to elevated insulin-like growth factor I receptor and initial modulation of the AKT pathway define the responsiveness of rhabdomyosarcoma to the targeting antibody
    Liang Cao
    Genetics Branch, Center for Cancer Research, Center for Cancer Research, National Cancer Institute, Bethesda, Maryland 20892 4265, USA
    Cancer Res 68:8039-48. 2008
    ..Subsequent progression of tumors was associated with reactivation of p-AKT despite sustained suppression of IGF-IR. These results identified the first predictive biomarker for anti-IGF-IR therapies in cancer...
  64. ncbi Structural evidence for recognition of a single epitope by two distinct antibodies
    D Fleury
    Laboratoire d Enzymologie et Biochimie Structurales, CNRS, France
    Proteins 40:572-8. 2000
    ..by residues of the H3 complementarity-determining regions (CDR) which adopt distinct conformations in the two antibodies. This demonstrates that there is a definite flexibility in the selection of antibodies that bind to a given ..
  65. doi Mechanisms of disease: aquaporin-4 antibodies in neuromyelitis optica
    Sven Jarius
    Weatherall Institute of Molecular Medicine, University of Oxford, UK
    Nat Clin Pract Neurol 4:202-14. 2008
    ..In NMO, serum antibodies, collectively known as NMO-IgG, characteristically bind to cerebral microvessels, pia mater and Virchow-Robin ..
  66. pmc Directed evolution of a secretory leader for the improved expression of heterologous proteins and full-length antibodies in Saccharomyces cerevisiae
    J Andy Rakestraw
    Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Biotechnol Bioeng 103:1192-201. 2009
    ....
  67. doi Antibodies against deamidated gliadin peptides identify adult coeliac disease patients negative for antibodies against endomysium and tissue transglutaminase
    C Dahle
    Department of Clinical Immunology and Transfusion Medicine, Linkoping University Hospital, Linkoping, Sweden
    Aliment Pharmacol Ther 32:254-60. 2010
    This study was done to evaluate the diagnostic utility of antibodies against deamidated gliadin peptides compared to traditional markers for coeliac disease.
  68. doi AQP4 antibodies in neuromyelitis optica: diagnostic and pathogenetic relevance
    Sven Jarius
    Division of Molecular Neuroimmunology, Department of Neurology, University of Heidelberg, Im Neuenheimer Feld 400, 69120 Heidelberg, Germany
    Nat Rev Neurol 6:383-92. 2010
    b>Antibodies to aquaporin-4 (also known as AQP4-Ab or NMO-IgG) are sensitive and highly specific serum markers of autoimmune neuromyelitis optica (NMO)...
  69. pmc Antibodies to Kv1 potassium channel-complex proteins leucine-rich, glioma inactivated 1 protein and contactin-associated protein-2 in limbic encephalitis, Morvan's syndrome and acquired neuromyotonia
    Sarosh R Irani
    Neurosciences Group, Department of Clinical Neurology, University of Oxford, John Radcliffe Hospital, Oxford, UK
    Brain 133:2734-48. 2010
    b>Antibodies that immunoprecipitate (125)I-alpha-dendrotoxin-labelled voltage-gated potassium channels extracted from mammalian brain tissue have been identified in patients with neuromyotonia, Morvan's syndrome, limbic encephalitis and a ..
  70. doi Cut-off levels and diagnostic accuracy of infliximab trough levels and anti-infliximab antibodies in Crohn's disease
    Casper Steenholdt
    Department of Medical Gastroenterology, University Hospital of Copenhagen, Herlev Hospital, Herlev, Denmark
    Scand J Gastroenterol 46:310-8. 2011
    ..Serum levels of infliximab and anti-infliximab antibodies have been associated with loss of response...
  71. pmc Engineered single-domain antibodies with high protease resistance and thermal stability
    Greg Hussack
    Institute for Biological Sciences, National Research Council Canada, Ottawa, Ontario, Canada
    PLoS ONE 6:e28218. 2011
    ..the upper gastrointestinal tract is a major obstacle facing orally-administered protein therapeutics, including antibodies. Through protein engineering, several Clostridium difficile toxin A-specific heavy chain antibody variable ..
  72. pmc Skin langerin+ dendritic cells transport intradermally injected anti-DEC-205 antibodies but are not essential for subsequent cytotoxic CD8+ T cell responses
    Vincent Flacher
    Department of Dermatology and Venereology, Innsbruck Medical University, A 6020 Innsbruck, Austria
    J Immunol 188:2146-55. 2012
    ....
  73. doi A highly versatile microscope imaging technology platform for the multiplex real-time detection of biomolecules and autoimmune antibodies
    Stefan Rödiger
    Lausitz University of Applied Sciences, Senftenberg, Germany
    Adv Biochem Eng Biotechnol 133:35-74. 2013
    ..Microbead-based and solution applications for the detection of diverse biomolecules, including antigens, antibodies, peptides, oligonucleotides and amplicons in small reaction volumes, are presented together with a high-content ..
  74. doi Avian IgY antibodies and their recombinant equivalents in research, diagnostics and therapy
    Edzard Spillner
    Institute of Biochemistry and Molecular Biology, Department of Chemistry, University of Hamburg, Martin Luther King Platz 6, 20146 Hamburg, Germany
    Biologicals 40:313-22. 2012
    The generation and use of avian antibodies is of increasing interest in a wide variety of applications within the life sciences...
  75. pmc A genome-wide integrative genomic study localizes genetic factors influencing antibodies against Epstein-Barr virus nuclear antigen 1 (EBNA-1)
    Rohina Rubicz
    Department of Genetics, Texas Biomedical Research Institute, San Antonio, Texas, United States of America
    PLoS Genet 9:e1003147. 2013
    ..order to localize genetic factors influencing levels of Epstein Barr virus (EBV) nuclear antigen-1 (EBNA-1) IgG antibodies, as a measure of history of infection with this pathogen, in large Mexican American families...
  76. doi Complement-binding anti-HLA antibodies and kidney-allograft survival
    Alexandre Loupy
    Paris Translational Research Center for Organ Transplantation, INSERM Unité 970, Department of Kidney Transplantation, Hopital Necker, Universite Paris Descartes, and Assistance Publique Hôpitaux de Paris AP HP, Paris, France
    N Engl J Med 369:1215-26. 2013
    Anti-HLA antibodies hamper successful transplantation, and activation of the complement cascade is involved in antibody-mediated rejection...
  77. pmc Induction of immune tolerance to coagulation factor IX antigen by in vivo hepatic gene transfer
    Federico Mingozzi
    Department of Pediatrics, The Children s Hospital of Philadelphia, University of Pennsylvania Medical Center, Philadelphia, Pennsylvania 19104, USA
    J Clin Invest 111:1347-56. 2003
    ..With a strain-dependent rate of success, tolerance to murine F.IX was induced in mice with a large F.IX gene deletion, supporting the relevance of these data for treatment of hemophilia B and other genetic diseases...
  78. ncbi Domain antibodies: proteins for therapy
    Lucy J Holt
    Domantis Limited, Granta Park, Abington, Cambridge CB1 6GS, UK
    Trends Biotechnol 21:484-90. 2003
    Occurring naturally in "heavy chain" immunoglobulins from camels, and now produced in fully human form, domain antibodies (dAbs) are the smallest known antigen-binding fragments of antibodies, ranging from 11 kDa to 15 kDa...
  79. ncbi Hepatic adducts, circulating antibodies, and cytokine polymorphisms in patients with diclofenac hepatotoxicity
    Guruprasad P Aithal
    School of Clinical Medical Sciences Hepatology, Medical School, University of Newcastle, Newcastle upon Tyne, United Kingdom
    Hepatology 39:1430-40. 2004
    ..The purpose of the present study was to explore the potential role played by the immune processes. Antibodies to diclofenac metabolite-modified liver protein adducts were detected in the sera of seven out of seven patients ..
  80. ncbi An open letter to our readers on the use of antibodies
    Clifford B Saper
    J Comp Neurol 493:477-8. 2005
  81. doi Should we be cautious on the use of commercially available antibodies to dopamine receptors?
    Serena Bodei
    Section of Pharmacology, Department of Biomedical Sciences and Biotechnologies, University of Brescia Medical School, V le Europa 11, 25124, Brescia, Italy
    Naunyn Schmiedebergs Arch Pharmacol 379:413-5. 2009
    Evidence indicate that it is difficult to obtain specific antibodies to G protein-coupled receptors and different technical difficulties may allow the generation of antibodies that lack specificity...
  82. doi Lack of specificity of antibodies directed against human beta-adrenergic receptors
    Nazha Hamdani
    Laboratory for Physiology, Institute for Cardiovascular Research, VU University Medical Center, Van der Boechorststraat 7, 1081 BT, Amsterdam, The Netherlands
    Naunyn Schmiedebergs Arch Pharmacol 379:403-7. 2009
    The present study was designed to investigate if antibodies against beta-adrenergic receptors (betaARs) can be used to determine expression of betaAR in human myocardium...
  83. doi Additional value of EGFR downstream signaling phosphoprotein expression to KRAS status for response to anti-EGFR antibodies in colorectal cancer
    Geraldine Perkins
    Institut National de la Recherche et de la Santé Médicale, UMR S775, Paris, France
    Int J Cancer 127:1321-31. 2010
    KRAS mutations are a strong predictive marker of resistance to anti-epidermal growth factor receptor (EGFR) antibodies in advanced colorectal cancer (CRC) but only a subset of wild-type (WT) KRAS patients are responders, suggesting the ..
  84. pmc Using simple artificial intelligence methods for predicting amyloidogenesis in antibodies
    Maria Pamela C David
    Virtual Laboratory of Biomolecular Structures, Marine Science Institute, College of Science, University of the Philippines Diliman, Quezon City 1101, Philippines
    BMC Bioinformatics 11:79. 2010
    ..We explore using a naive Bayesian classifier and a weighted decision tree for predicting the amyloidogenicity of immunoglobulin sequences...
  85. doi Antibodies for profiling the human proteome-The Human Protein Atlas as a resource for cancer research
    Anna Asplund
    Science for Life Laboratory, Department of Immunology, Genetics and Pathology, Uppsala University, Uppsala, Sweden
    Proteomics 12:2067-77. 2012
    ..The objectives underlying the Human Protein Atlas include (i) the generation of validated antibodies toward a major isoform of all proteins encoded by the human genome, (ii) creating an information database of ..
  86. pmc Setting of methods for analysis of mucosal antibodies in seminal and vaginal fluids of HIV seropositive subjects from Cambodian and Italian cohorts
    Carla Donadoni
    Division of Immunology, Infectious Diseases and Trasplantation, San Raffaele Scientific Institute, Milan, Italy
    PLoS ONE 5:e9920. 2010
    ..High variability of published observations concerning IgA isolation and quantification underlies the strong requirement of specific methods able to maximize investigation on HIV-specific IgA...
  87. pmc Quantitative assessment of chromatin immunoprecipitation grade antibodies directed against histone modifications reveals patterns of co-occurring marks on histone protein molecules
    Sally E Peach
    Broad Institute, Proteomics Platform, Cambridge, Massachusetts 02142, USA
    Mol Cell Proteomics 11:128-37. 2012
    ..method using mass spectrometry to characterize the specificity of key histone H3 modification-targeting antibodies that have previously been used to characterize the "histone code...
  88. doi A proteomics approach for the identification and cloning of monoclonal antibodies from serum
    Wan Cheung Cheung
    Cell Signaling Technology, Inc, Danvers, Massachusetts, USA
    Nat Biotechnol 30:447-52. 2012
    ..a proteomics approach that identifies antigen-specific antibody sequences directly from circulating polyclonal antibodies in the serum of an immunized animal...
  89. doi Association, prevalence, and clearance of human papillomavirus and antisperm antibodies in infected semen samples from infertile patients
    Andrea Garolla
    Unit for Human Reproduction Pathology, Section of Clinical Pathology, Department of Molecular Medicine, University of Padova, Italy
    Fertil Steril 99:125-31. 2013
    To evaluate prevalence, association, and clearance of human papillomavirus (HPV) and antisperm antibodies (ASAs) in infected semen samples from infertile patients.
  90. pmc Antibodies covalently immobilized on actin filaments for fast myosin driven analyte transport
    Saroj Kumar
    School of Natural Sciences, Linnaeus University, Kalmar, Sweden
    PLoS ONE 7:e46298. 2012
    ..necessary requirement for the use of the actomyosin system in biosensing devices, namely covalent attachment of antibodies to actin filaments using heterobifunctional cross-linkers...
  91. pmc Activation-induced deaminase-deficient MRL/lpr mice secrete high levels of protective antibodies against lupus nephritis
    Chuancang Jiang
    National Institute of Environmental Health Sciences, Research Triangle Park, NC, USA
    Arthritis Rheum 63:1086-96. 2011
    ..The purpose of the present study was to test the hypothesis that high levels of germline autoreactive IgM in these mice confer protection against lupus nephritis...
  92. pmc The association of heavy and light chain variable domains in antibodies: implications for antigen specificity
    Anna Chailyan
    Department of Physics, Sapienza University of Rome, Italy
    FEBS J 278:2858-66. 2011
    ..We also show that the different packing modes are related to the type of recognized antigen...
  93. pmc Anti-Anopheles darlingi saliva antibodies as marker of Plasmodium vivax infection and clinical immunity in the Brazilian Amazon
    Bruno Bezerril Andrade
    Centro de Pesquisas Gonçalo Moniz FIOCRUZ, Bahia, Brazil
    Malar J 8:121. 2009
    ..darlingi saliva in volunteers from the Brazilian Amazon and addresses the association between levels of specific antibodies and clinical presentation of Plasmodium (P.) vivax infection.
  94. doi In vivo cytotoxicity of type I CD20 antibodies critically depends on Fc receptor ITAM signaling
    Simone de Haij
    Genmab, Immunotherapy Laboratory, Department of Immunology, University Medical Center, Utrecht, The Netherlands
    Cancer Res 70:3209-17. 2010
    Antibody-Fc receptor (FcR) interactions play an important role in the mechanism of action of most therapeutic antibodies against cancer...
  95. doi Cancer therapy with trifunctional antibodies: linking innate and adaptive immunity
    Juergen Hess
    TRION Pharma GmbH, Frankfurter Ring 193a, 80807 Munich, Germany
    Future Oncol 8:73-85. 2012
    Trifunctional antibodies (trAbs) are promising novel anticancer biologics with a particular mode of action capable of linking innate with adaptive immunity...
  96. pmc GABA(B) receptor antibodies in limbic encephalitis and anti-GAD-associated neurologic disorders
    A Boronat
    Servei de Neurologia, Hospital Clinic, Villarroel 170, Barcelona 08036, Spain
    Neurology 76:795-800. 2011
    Aminobutyric acid-B receptor antibodies (GABA(B)R-ab) were recently described in 15 patients with limbic encephalitis (LE), associated with small-cell lung cancer (SCLC) or with concurrent glutamic acid decarboxylase (GAD) antibodies...
  97. doi Binding kinetics of antiricin single domain antibodies and improved detection using a B chain specific binder
    George P Anderson
    Center for Bio Molecular Science and Engineering, Naval Research Laboratory, 4555 Overlook Avenue SW, Washington, DC 20375, USA
    Anal Chem 82:7202-7. 2010
    Single domain antibodies are the recombinantly expressed binding fragments derived from heavy chain antibodies found in camels and llamas...
  98. pmc Llama-derived single-domain antibodies for the detection of botulinum A neurotoxin
    Marla D Swain
    Center for Bio Molecular Science and Engineering, Naval Research Laboratory, 4555 Overlook Ave SW, Washington, DC 20375, USA
    Anal Bioanal Chem 398:339-48. 2010
    Single-domain antibodies (sdAb) specific for botulinum neurotoxin serotype A (BoNT A) were selected from an immune llama phage display library derived from a llama that was immunized with BoNT A toxoid...
  99. ncbi Paraneoplastic syndromes involving the nervous system
    Robert B Darnell
    Howard Hughes Medical Institute, Rockefeller University, New York, USA
    N Engl J Med 349:1543-54. 2003
  100. ncbi Heavy-chain antibodies in Camelidae; a case of evolutionary innovation
    Viet Khong Nguyen
    Department of Ultrastructure, Vlaams Interuniversitaire Instituut voor Biotechnologie, Vrije Universiteit Brussel, Belgium
    Immunogenetics 54:39-47. 2002
    The emergence in Camelidae species of functional antibodies devoid of light chains (referred to as heavy-chain antibodies or HCAbs) is an intriguing evolutionary event...
  101. pmc Induction of dermal-epidermal separation in mice by passive transfer of antibodies specific to type VII collagen
    Cassian Sitaru
    Department of Dermatology, University of Lubeck, Lubeck, Germany
    J Clin Invest 115:870-8. 2005
    ..To study the pathogenic relevance of antibodies specific to type VII collagen in vivo, we generated and characterized rabbit antibodies specific to a murine ..

Research Grants84

  1. Discovery metabolite profiling of the prolyl peptidases
    Alan Saghatelian; Fiscal Year: 2009
    ..Furthermore, the application of DMP to peptidases will demonstrate the generality of this approach for the future characterization of medically relevant enzymes and signaling pathways. ..
  2. Personalized Clinical Diagnostics and Beyond: Integrated Ring Resonator Arrays
    Ryan Bailey; Fiscal Year: 2009
    ..This technology promises to broadly impact the landscape of the biomedical sciences, both meeting the clinical diagnostic challenges of today and pioneering the paradigm-shifting discoveries of tomorrow. ..
  3. CFI Pathogen Inactivation Technology
    Trevor P Castor; Fiscal Year: 2010
    ..Some enzyme immunoassays used for routine screening may detect viral antigens or antibodies, but not the infectious agents themselves...
  4. Ian C Michelow; Fiscal Year: 2016
    ..the hypothesis that novel vaccine targets can be discovered by identifying transplacentally acquired malarial antibodies that mediate infants'resistance to severe or fatal malaria...
  5. ASTHMA AND ALLERGIC DISEASE CENTER
    Thomas A Platts Mills; Fiscal Year: 2010
    ..use new techniques to measure allergen and endotoxin exposure in homes, and a novel approach to measuring IgE antibodies specific for airborne particles...
  6. Cardiotrophin-Like Cytokine 1, a Candidate Molecule for the FSGS Factor
    Virginia J Savin; Fiscal Year: 2010
    ..the striking ability of anti-CLC-1 monoclonal antibodies to block the permeability effect of FSGS plasma...
  7. Cross-talk Between Coagulation, Inflammation, and Immunity
    Lawrence L Leung; Fiscal Year: 2013
    ..To determine the relevant forms of active chemerin in vivo, we have generated polyclonal antibodies specific for prochemerin and plasmin-cleaved and plasmin/CPB-double cleaved chemerin...
  8. Importance of antigen specific immunoglobulin responses in controlling SIV/SHIV i
    Bapi Pahar; Fiscal Year: 2010
    ..also play an important role in the immune response to infection by secreting neutralizing and non-neutralizing antibodies to combat invading pathogens...
  9. Emilio B Gonzalez; Fiscal Year: 2014
    DESCRIPTION (provided by applicant): Antiphospholipid (aPL) antibodies (Abs) are associated with thrombosis and pregnancy loss in patients with systemic lupus erythematosus (SLE) and antiphospholipid syndrome (APS)...
  10. Optimized Growth Media to Detect Intracellular Bacteria Implicated in Chronic Dis
    PATRICIA MASCARELLI; Fiscal Year: 2011
    ..high false negative rates for direct PCR and that the host immune system has little opportunity to develop antibodies against this bacteria...
  11. Gamma-Synuclein-Mediated Regulation of Norepinephrine Transporter
    Anita Sidhu; Fiscal Year: 2010
    ..will be analyzed by pulse-chase labeling using [3H]methionine followed by immunoprecipitation with -Syn antibodies. mRNA levels will be assessed by real-time RTPCR and mRNA stability will be analyzed after blockade of RNA ..
  12. ANTHONY JOHN KANAI; Fiscal Year: 2016
    ..benefits of nitro-fatty acids (FA-NO2) and brain-derived neurotrophic factor- and nerve growth factor-antibodies (BDNF-AB and NGF-AB) will be tested...
  13. Regulation of B Cell Function by Membrane-Cytoskeletal Remodeling
    Neetu Gupta; Fiscal Year: 2012
    ..Health Relevance: B cells respond to environmental pathogens such as bacteria and viruses by secreting specific antibodies that clear infections. This provides the basis for memory that is generated during early childhood vaccinations...
  14. Craig T Morita; Fiscal Year: 2016
    ..V32V42 T cells also express the CD16 immunoglobulin Fc receptor, they kill tumor cells sensitized by anti-tumor antibodies. In recent years, immunotherapy with prenyl pyrophosphates or bisphosphonates and IL-2 to stimulate V32V42 T ..
  15. Neil W Kowall; Fiscal Year: 2014
    ..assessing transcriptional activity using a luciferase assay and the methylation of histone H3K4 using specific antibodies. We will determine the effect of spermidine and tranylcypromine, an LSD1 inhibitor on LSD1 activity and DMH3K4 ..
  16. Integrated Microfluidic Platform for Point-of-Care Serodiagnostics
    Xiaowu Liang; Fiscal Year: 2013
    ..Additionally, ELISAs rely on the inherent humoral immune response of the host to produce antibodies indicative of pathological status...
  17. Statin modulation of immunotherapy for Alzheimer disease
    Jun Tan; Fiscal Year: 2012
    ..form this trial suggested some success in slowing the rate of dementia progression in patients generating antibodies against brain A(...
  18. IMMUNOGENETICS OF COLLAGEN INDUCED ARTHRITIS IN MICE
    Chella David; Fiscal Year: 2009
    ..CIA) susceptible HLA transgenic mice produce rheumatoid factor and anti-cyclic citrullinated peptide (CCP) antibodies similar to that in RA patients...
  19. Terence S Dermody; Fiscal Year: 2015
    ..Candidate receptors will be validated using receptor-specific antibodies, receptor-deficient cell lines, and assays of virus-receptor binding...
  20. SUPPORT CONTRACT FOR THE NTP INTERAGENCY CENTER/VALIDATION OF ALT METHODS
    David Allen; Fiscal Year: 2009
    ..include vaccines, blood and blood components, allergenics, somatic cells, gene therapy, tissues, monoclonal antibodies, and recombinant therapeutic proteins that are used to treat or protect humans or animals...
  21. Inhibition of IkK to treat lethal Graft-vs.-Host Disease
    Patrick Flood; Fiscal Year: 2009
    ..drugs such as methotrexate and a calcineuring inhibitor or in vivo methods of T cell depletion using antibodies such as Campath -1H...
  22. Chronic Sleep Restriction Increases Immunity to Autoantigen: Role of the SNS
    Arnold E Postlethwaite; Fiscal Year: 2010
    ..is enhanced with increased production of interferon3 and other Th1 cytokines, increased serum levels of anti-CII antibodies, decreased CD8+ T cells, increased CD11b+ cells and changes in plasma levels of inflammatory cytokines and ..
  23. Steroid Responsive Mechanisms in the Ear
    DENNIS ROYAL TRUNE; Fiscal Year: 2012
    ..systemic autoimmune disease will be assessed by serum immune complexes, hematocrits, and antinuclear antibodies;4) cochlearspecificautoantibodies willbeassessedwithELISA,and5)steroid-mediatedcochleargeneproducts will be ..
  24. Novel DNA-Launched Attenuated Vaccine for VEE Virus
    PETER M PUSHKO; Fiscal Year: 2012
    ..The vaccine causes adverse effects, whereas some individuals do not develop neutralizing antibodies. The efforts to develop new VEEV vaccines are underway...
  25. Bi-functional Therapeutics for Allergy-BFTA
    Swey Shen Chen; Fiscal Year: 2010
    ..Further, antibodies directed to C(2-3L constrained within the (-barrel of GFP, inhibit IgE binding to Fc(RI( and prevent mast cell ..
  26. Regulation of Airway Mucin Gene Expression by Epigenetic Mechanism
    Reen Wu; Fiscal Year: 2013
    ..immunoprecipitation (ChIP) approaches with anti-methylated deoxy-cytosine and various anti-modified histone antibodies will be used to assess DNA methylation profiles and chromatin structure of MUC5AC...
  27. Susan L Deutscher; Fiscal Year: 2016
    ..Radiolabeled antibodies and peptides are currently being explored as diagnostic cancer imaging agents...
  28. Dengue Infected Endothelial Cells Enhance Immune Cell Activation
    Erich R Mackow; Fiscal Year: 2013
    ..found in DHF/DSS result from vascular leakage, and these severe manifestations are enhanced by preexisting DV antibodies and subsequent infection by a second DV serotype...
  29. UTPAL BHAGIRATH PAJVANI; Fiscal Year: 2015
    ..the effects of inhibition of Notch signaling with pharmacologic tools (small molecule inhibitors or monoclonal antibodies) or other genetic mouse models of Notch hypofunction. Additionally, Dr...
  30. Global regulatory interactions in bacterial pathogenesis
    BRIAN AKERLEY; Fiscal Year: 2009
    ..pathogenesis by promoting adherence to host cells, yet is also a target for host immune effectors such as innate antibodies and C-reactive protein...
  31. Cellular Immunity to Category A-C Viruses in Humans
    ROBERT WILLIAM FINBERG; Fiscal Year: 2013
    ..Influenza A virus hemagglutinin (HA) is a target of protective neutralizing antibodies, which are subtypespecific and vulnerable to antigenic drift...
  32. Novel, Rapidly Translatable Technologies for Healing Long Bone Segmental Defects
    Christopher H Evans; Fiscal Year: 2010
    ..Sections of the healed rat femora will be stained immunohistochemically with antibodies that recognize human, but not rat, nuclear antigen and thus stain all human nuclei...
  33. Christie P Thomas; Fiscal Year: 2014
    ..the proteinuria and hypertension frequently seen in patients undergoing chemotherapy with anti-VEGF monoclonal antibodies or with tyrosine kinase inhibitors and provides a model to study the role of VEGF and its receptors in ..
  34. Novel diagnostic and safety biomarkers of liver injury and hepatotoxicity
    Stanislav I Svetlov; Fiscal Year: 2010
    ..Sufficient supplies of the assay components (e.g. antibodies and antigens) will be produced under quality assured conditions...
  35. Brian F Volkman; Fiscal Year: 2016
    ..Treatment with CXCR4-neutralizing antibodies reduces metastatic tumor formation in a mouse model for human breast cancer...
  36. Present Homologous and Heterologous Antigen with Hepatitis E Virus
    R Holland Cheng; Fiscal Year: 2012
    ..insertion of 15 amino acids at an antibody-binding site, the chimeric VLP reduces the reactivity to anti-HEV antibodies meanwhile retains the icosahedral assembly...
  37. Predictors of Pregnancy Outcome in SLE and APS
    Jane E Salmon; Fiscal Year: 2012
    ..elevations of complement split products predict pregnancy complications in patients with antiphospholipid (aPL) antibodies and/or SLE...
  38. SAUL R TZIPORI; Fiscal Year: 2014
    ..Protection against CDI was shown to be mediated through systemic and mucosal antibodies against the 2 key toxins, although other virulence attributes are known to exist which may also contribute to ..
  39. JEAN CLAIRE LEE; Fiscal Year: 2014
    ..High titered rabbit antibodies to CP5-EPA promoted opsonophagocytic killing of two different S...
  40. Rafi Ahmed; Fiscal Year: 2016
    ..Immune Senescence (Goronzy), and a Technology Development Project on Human Monoclonal Antibodies (Wilson/Lanzavecchia)...
  41. JUSTIN RICHARD BAILEY; Fiscal Year: 2016
    ..the fitness costs of those mutations, and to define the human B cell repertoire encoding broadly neutralizing antibodies induced during HCV infection...
  42. ALAN AI PING WEI; Fiscal Year: 2014
    ..A PB is used as a fluorogenic tracer in competition with aflatoxin for binding to anti-aflatoxin antibodies. The PB will be non-fluorescent when bound to the antibody but becomes highly fluorescent when displaced by AF, ..
  43. Novel Macrolide Th17 Inhibitors
    Jay K Kolls; Fiscal Year: 2012
    ..Anti-IL-12p40 antibodies (Ustekinumab) which suppress Th17 cytokine elaboration have recently been approved for the treatment of ..
  44. Non-coding RNA engineers antibody diversity
    Uttiya Basu; Fiscal Year: 2011
    ..b>Antibodies are polypeptide complexes produced from B-lymphocytes that are present in the bodily fluids of vertebrates, and ..
  45. Christopher D Heaney; Fiscal Year: 2014
    ..Weber and Schmitz to develop, optimize and validate an ELISA for detection of hepatitis E virus (HEV) antibodies (anti-HEV) in saliva. For this purpose co-mentor Dr...
  46. Characterization of epsilon-sarcoglycan interacting proteins in mouse brain
    Yuqing Li; Fiscal Year: 2012
    ..from WT and Sgce KO mouse brains and analyze the levels of the interacting proteins by Western blot with antibodies against the identified proteins...
  47. Novel Therapy for Preeclampsia
    James W Larrick; Fiscal Year: 2012
    ..level of an endogenous "digoxin-like" factor (EDLF), an unknown substance that cross reacts with anti-digoxin antibodies and inhibits the Na+/K+ ATPase (NKA) was first noted in the 1980s...
  48. MOLECULAR BIOLOGY OF HUMAN COAGULATION FACTOR V
    Thomas Ortel; Fiscal Year: 2010
    ..Binding sites will be defined using recombinant factor Va mutants, recombinant light chain domains, scFv antibodies and soluble phospholipid analogues...
  49. Knockdown of Gene Expression in Immune Cells in Humanized Mice
    Judy Lieberman; Fiscal Year: 2010
    ..Experimental manipulations (i.e. gene knockout, blocking antibodies, and cytokines) that have been so powerful for dissecting the molecular basis of mouse immunology are rarely ..
  50. Extracellular ubiquitin: role in myocyte apoptosis and myocardial remodeling
    Krishna Singh; Fiscal Year: 2010
    ..Aim 1 will determine the specificity of extracellular ubiquitin in ?-AR-stimulated apoptosis using neutralizing antibodies against ubiquitin, and other apoptotic agents (angiotensin II, oxidative stress and Tumor necrosis factor-1), ..
  51. Robust Peptide-Based Diagnostics of Botulinum Toxins
    John E Mueller; Fiscal Year: 2012
    ..are confounded by one or the other of the following requirements: 1) expensive and/or sensitive reagents (antibodies);that require stringent storage (e.g...
  52. Vitamin A adjuvant to enhance gut immunity and rotavirus vaccines in neonates
    Linda J Saif; Fiscal Year: 2010
    ..In infants and piglets, fecal or serum IgA antibodies or IgA antibody secreting cells in the gut correlate with protection against RV diarrhea...
  53. SATDARSHAN SINGH MONGA; Fiscal Year: 2016
    ..novel transgenic mice in our lab and complemented by utilization of species-specific PDGFR[unreadable] blocking antibodies to determine impact on disease progression in these models to address therapeutic efficacy...
  54. New Rapid Diagnostic Tests for Amebiasis
    JOEL F HERBEIN; Fiscal Year: 2010
    ..HISTOLYTICA SERUM ANTIBODY QUIK CHEK test, which is designed to detect anti-E. histolytica antibodies in serum samples of patients exposed to E. histolytica who are antigen negative...
  55. Anti-Fibrotic Therapy for Scleroderma/SSc
    Latha Paka; Fiscal Year: 2010
    ..Furthermore, the possibility of PDGFR activation through auto-antibodies is a novel and provocative concept in autoimmune fibrotic diseases...
  56. B Cell Development in Aging
    John C Cambier; Fiscal Year: 2010
    ..Multiple acquired defects may contribute to the reduced ability of elderly individuals to produce protective antibodies against newly encountered pathogens...
  57. MINORITY PREDOCTORAL FELLOWSHIP PROGRAM
    Quentin J Baca; Fiscal Year: 2010
    ..At Harvard Mr. Baca did 2 rotations first in Thomas Look's lab working on making antibodies to oncogenic transfection factors involved in Human leukemias and then in Dr...
  58. Bingmei M Fu; Fiscal Year: 2014
    ..and a) under conditions of normal and increased permeability by VEGF, b) after pretreatment with the blocking antibodies to endothelial cell adhesion molecules, c) after pretreatment with the blocking antibodies to tumor cell ..
  59. Evolution of the Immune Response to Cytomegalovirus Transmitted via Breast Milk
    Lynn Puddington; Fiscal Year: 2010
    ..As breast milk is enriched with monocytes/macrophages and HCMV-specific antibodies, it is possible that these maternal antibodies can influence postnatal infection through the gastrointestinal ..
  60. Olaf Schneewind; Fiscal Year: 2016
    ..proteins IsdA and IsdB, generated significant protective immunity that correlated with the induction of specific antibodies. When assembled into a combined vaccine, the surface protein vaccine afforded high levels of protection against ..
  61. Oliver W Press; Fiscal Year: 2014
    ..Radiolabeled anti-CD45 monoclonal antibodies (Ab) have been shown to improve outcomes for AML and MDS in the setting of HCT, but toxicity remains high and ..
  62. Mucosal Immunity and Influenza Vaccines: Phenotype and Role of Activated B Cells
    HARRY BERNARD GREENBERG; Fiscal Year: 2010
    ..analyses of B cell immunity after influenza vaccination have been largely limited to studies of serum antibodies, which do not provide adequate or unified markers for the efficacy of LAIV and TIV...
  63. Lih Yun Hsu; Fiscal Year: 2016
    ..YYAA mice, like SKG mice, develop rheumatoid factor antibodies, but fail to develop autoimmune arthritis...
  64. GENETIC ANALYSIS OF LUPUS AUTOIMMUNITY
    Judith James; Fiscal Year: 2000
    ..with specific short peptides derived from the Sm autoantigen develop high levels of both anti-Sm and anti-nRNP antibodies; the peptides used as immunogens were selected since they are also bound by human lupus autoantibodies...
  65. Synaptic autoimmunity in disorders of memory, behavior, cognition and psychosis
    Josep Dalmau; Fiscal Year: 2010
    ..Using techniques that we optimized to detect antibodies to neuronal cell surface and/or synaptic proteins, we found that all patients had antibodies against the NR1 ..
  66. ANTIBODIES WITH PROTHROMBINASE ACTIVITY IN LUPUS
    Perumal Thiagarajan; Fiscal Year: 2003
    ..In Preliminary Studies, we identified a new and potent mechanism by which these antibodies can induce thrombosis, i.e. the catalytic cleavage of prothrombin...
  67. Generation of monoclonal and polyclonal antibodies to neolacto-series ganglioside
    Amjad A Ilyas; Fiscal Year: 2010
    ..Gangliosides have been implicated as antigens in various autoimmune neuropathies. For example, anti-GM1 antibodies are associated with motor neuropathy, anti-GD1a antibodies are associated with acute motor axonal neuropathy, ..
  68. COLLAGENS OF HYALINE CARTILAGE
    Richard Mayne; Fiscal Year: 2002
    ..Extensive use will be made to high affinity monoclonal antibodies (mAbs) specific for each collagen type for which the location of different epitopes along the collagen molecule ..
  69. High Mannose Type Carbohydrate-based HIV/AIDS Vaccine
    Yu Geng; Fiscal Year: 2009
    ..few of the vaccine candidates currently in preclinical and clinical trials appear to elicit broadly neutralizing antibodies. Several lines of evidences show that high mannose type carbohydrates on the HIV-1 glycoprotein gp120 are a ..
  70. Glycosylation of full-length antibodies in Escherichia coli
    ADAM FISHER; Fiscal Year: 2009
    Currently, therapeutic antibodies represent approximately 30% of all biotech revenues (>$35 billion) and nearly all are produced in mammalian cell culture...
  71. Mechanisms of non-neutralizing antibody-mediated protection from influenza virus
    DENISE KAMINSKI; Fiscal Year: 2009
    ..However, current influenza vaccines mostly induce antibodies to the virus'external glycoproteins...
  72. Structure Based Design of Humanized Monoclonal Antibodies that Recognize Bioactiv
    JONATHAN WOJCIAK; Fiscal Year: 2009
    Currently, there are over 20 therapeutic antibodies on the market. It is the fastest growing segment of therapeutics largely because humanized mAbs have excellent safety profiles...
  73. SIMPLIFIED POPULATION SCREENING FOR ADULT HYPOTHYROIDISM
    Edwin Naylor; Fiscal Year: 2000
    ..and validated sensitive and precise methods to measure thyroid stimulating hormone (TSH)and the thyroid antibodies, thyroperoxidase antibodies (TPOAb) and thyroglobulin antibodies (TGAb)in DBS specimens...
  74. Broad-Spectrum Therapeutic Human Antibodies for Dengue Virus Infections
    Yorgo Modis; Fiscal Year: 2006
    ..Our working hypothesis is that antibodies recognizing specific, highly conserved envelope protein epitopes can prevent and treat infection by several ..
  75. Immunoglobulin CDR-H3 and neutralizing antibodies to HIV
    Harry Schroeder; Fiscal Year: 2009
    DESCRIPTION (provided by applicant): Among neutralizing antibodies (Nabs) directed against the HIV-1 envelope gp120/gp41 glycoprotein, 2F5 and 4E10 stand out for their potency and broadly neutralizing activity...
  76. CTL2 ELISA: A Diagnostic Test for Autoimmune Hearing Loss
    Vijay Kumar; Fiscal Year: 2007
    ..Multiple lines of evidence implicate antibodies to a 68 kDa antigen in AHL...
  77. Human Autoantibodies in Anti-GBM Disease
    Anne Christensen; Fiscal Year: 2005
    ..provided by applicant):Anti glomerular basement membrane (GBM) disease is an autoimmune disease mediated by antibodies against the GBM...