eosinophil derived neurotoxin

Summary

Summary: A 19-kDa cationic peptide found in EOSINOPHIL granules. Eosinophil-derived neurotoxin is a RIBONUCLEASE and may play a role as an endogenous antiviral agent.

Top Publications

  1. ncbi Rapid evolution of a unique family of primate ribonuclease genes
    H F Rosenberg
    Laboratory of Host Defenses, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
    Nat Genet 10:219-23. 1995
  2. ncbi Eosinophils contribute to innate antiviral immunity and promote clearance of respiratory syncytial virus
    Simon Phipps
    Centre for Asthma and Respiratory Diseases CARD, School of Biomedical Sciences, University of Newcastle, Newcastle, Australia
    Blood 110:1578-86. 2007
  3. ncbi Localization of eosinophil-derived neurotoxin and eosinophil cationic protein in neutrophilic leukocytes
    S Sur
    Department of Immunology, Mayo Clinic and Mayo Foundation, and Mayo Medical School, Rochester, Minnesota 55905, USA
    J Leukoc Biol 63:715-22. 1998
  4. ncbi Urinary eosinophil-derived neurotoxin concentrations in patients with atopic dermatitis: a useful clinical marker for disease activity
    Tomoko Goto
    Gunma Institute for Allergy and Asthma, Shin Ohra Hospital, Gunma, Japan
    Allergol Int 56:433-8. 2007
  5. pmc Human ribonuclease A superfamily members, eosinophil-derived neurotoxin and pancreatic ribonuclease, induce dendritic cell maturation and activation
    De Yang
    Basic Research Program, Science Applications International Corporation SAIC Frederick, MD 21702, USA
    J Immunol 173:6134-42. 2004
  6. ncbi Urinary eosinophil protein X in relation to disease activity in childhood asthma
    E Lugosi
    Division of Allergy and Pulmonology, University Children s Hospital, Vienna, Austria
    Allergy 52:584-8. 1997
  7. ncbi Serum eosinophil derived neurotoxin may reflect more strongly disease severity in childhood atopic dermatitis than eosinophil cationic protein
    S Taniuchi
    Department of Paediatrics, Kansai Medical University, Fumizonocho 10 15, Moriguchi, 570 8506, Osaka, Japan
    J Dermatol Sci 26:79-82. 2001
  8. ncbi Eosinophils, eosinophil ribonucleases, and their role in host defense against respiratory virus pathogens
    H F Rosenberg
    Eosinophil Biology Unit, Laboratory of Host Defenses, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Leukoc Biol 70:691-8. 2001
  9. ncbi Genetic polymorphisms of eosinophil-derived neurotoxin and eosinophil cationic protein in tropical pulmonary eosinophilia
    Yae Jean Kim
    Laboratory of Malaria and Vector Research, Biostatistics Research Branch, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
    Am J Trop Med Hyg 73:125-30. 2005
  10. ncbi Eosinophil-derived neurotoxin (EDN), an antimicrobial protein with chemotactic activities for dendritic cells
    De Yang
    Basic Research Program, SAIC Frederick, Laboratory of Molecular Regulation, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bldg 560, Rm 31 19, Frederick, MD 21702 1201, USA
    Blood 102:3396-403. 2003

Research Grants

  1. Alternaria and ribonucleases in Th2-type immunity
    Hirohito Kita; Fiscal Year: 2013
  2. ANTIVIRAL EFFECTS OF EOSINOPHILS IN THE LUNGS
    David Jacoby; Fiscal Year: 2007
  3. Eosinophil Granule Proteins and Their Functions
    Gerald Gleich; Fiscal Year: 2006
  4. CARDIOTOXICITY OF EOSINOPHIL GRANULE CATIONIC PROTEINS
    Helene Rosenberg; Fiscal Year: 1991
  5. NOVEL THERAPY FOR KAPOSI'S SARCOMA
    JAMES LARRICK; Fiscal Year: 2000
  6. Global Gene Expression in Chronic Rhinosinusitis
    DOUGLAS PLAGER; Fiscal Year: 2005
  7. Ventilator-Induced Lung Injury in Children
    Sabrina Heidemann; Fiscal Year: 2004
  8. HIV and Neutrophil Function in Oral Disease
    Larry Thomas; Fiscal Year: 2003
  9. Neutrophils and HIV-1 infection
    Larry Thomas; Fiscal Year: 2006
  10. Urine Protein Biomarker Exploration in Ovarian Cancer
    Bin Ye; Fiscal Year: 2006

Detail Information

Publications184 found, 100 shown here

  1. ncbi Rapid evolution of a unique family of primate ribonuclease genes
    H F Rosenberg
    Laboratory of Host Defenses, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
    Nat Genet 10:219-23. 1995
    ..These results suggest that both EDN and ECP may be responding to unusual evolutionary constraints, which has prompted a reexamination of their physiologic function...
  2. ncbi Eosinophils contribute to innate antiviral immunity and promote clearance of respiratory syncytial virus
    Simon Phipps
    Centre for Asthma and Respiratory Diseases CARD, School of Biomedical Sciences, University of Newcastle, Newcastle, Australia
    Blood 110:1578-86. 2007
    ..Collectively, our results demonstrate that eosinophils can protect against RSV in vivo, as they promote virus clearance and may thus limit virus-induced lung dysfunction...
  3. ncbi Localization of eosinophil-derived neurotoxin and eosinophil cationic protein in neutrophilic leukocytes
    S Sur
    Department of Immunology, Mayo Clinic and Mayo Foundation, and Mayo Medical School, Rochester, Minnesota 55905, USA
    J Leukoc Biol 63:715-22. 1998
    ..Thus, caution must be exercised in interpreting the presence of EDN and ECP as specific markers of eosinophil-associated inflammation in human disease...
  4. ncbi Urinary eosinophil-derived neurotoxin concentrations in patients with atopic dermatitis: a useful clinical marker for disease activity
    Tomoko Goto
    Gunma Institute for Allergy and Asthma, Shin Ohra Hospital, Gunma, Japan
    Allergol Int 56:433-8. 2007
    ..It has been reported that measurements of eosinophil-derived neurotoxin (EDN) may be useful for identifying eosinophil activities in allergic diseases including atopic dermatitis...
  5. pmc Human ribonuclease A superfamily members, eosinophil-derived neurotoxin and pancreatic ribonuclease, induce dendritic cell maturation and activation
    De Yang
    Basic Research Program, Science Applications International Corporation SAIC Frederick, MD 21702, USA
    J Immunol 173:6134-42. 2004
    ..We therefore propose these mediators be considered as endogenous multifunctional immune alarmins...
  6. ncbi Urinary eosinophil protein X in relation to disease activity in childhood asthma
    E Lugosi
    Division of Allergy and Pulmonology, University Children s Hospital, Vienna, Austria
    Allergy 52:584-8. 1997
    ..Measurement of U-EPX may be useful in assessing the inflammatory process and therefore in the management of childhood asthma...
  7. ncbi Serum eosinophil derived neurotoxin may reflect more strongly disease severity in childhood atopic dermatitis than eosinophil cationic protein
    S Taniuchi
    Department of Paediatrics, Kansai Medical University, Fumizonocho 10 15, Moriguchi, 570 8506, Osaka, Japan
    J Dermatol Sci 26:79-82. 2001
    ..However, the relationship between the disease severity and the eosinophil derived neurotoxin (EDN) has not been established in AD patients...
  8. ncbi Eosinophils, eosinophil ribonucleases, and their role in host defense against respiratory virus pathogens
    H F Rosenberg
    Eosinophil Biology Unit, Laboratory of Host Defenses, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Leukoc Biol 70:691-8. 2001
    ....
  9. ncbi Genetic polymorphisms of eosinophil-derived neurotoxin and eosinophil cationic protein in tropical pulmonary eosinophilia
    Yae Jean Kim
    Laboratory of Malaria and Vector Research, Biostatistics Research Branch, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
    Am J Trop Med Hyg 73:125-30. 2005
    ..Although the EDN and ECP polymorphisms appear unlikely to be associated with the development of TPE, further analyses will be more definitive...
  10. ncbi Eosinophil-derived neurotoxin (EDN), an antimicrobial protein with chemotactic activities for dendritic cells
    De Yang
    Basic Research Program, SAIC Frederick, Laboratory of Molecular Regulation, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bldg 560, Rm 31 19, Frederick, MD 21702 1201, USA
    Blood 102:3396-403. 2003
    ..Thus, EDN and its mouse ortholog, mEAR2, are eosinophil granule-derived antimicrobial RNases that function as chemoattractants for DCs in vitro and in vivo...
  11. ncbi Multiple roles of antimicrobial defensins, cathelicidins, and eosinophil-derived neurotoxin in host defense
    De Yang
    Basic Research Program, National Cancer Institute at Frederick, National Institutes of Health, Frederick, MD 21702, USA
    Annu Rev Immunol 22:181-215. 2004
    ..Cathelicidins act on FPRL1-expressing cells. Furthermore, several defensins have considerable immunoenhancing activity. Thus, it appears that mammalian antimicrobial proteins contribute to both innate and adaptive antimicrobial immunity...
  12. ncbi A synthetic peptide derived from the human eosinophil-derived neurotoxin induces apoptosis in Kaposi's sarcoma cells
    Anica Dricu
    Department of Oncology Pathology, Cancer Centrum Karolinska, Karolinska Hospital, RS 00 17176, Stockholm, Sweden
    Anticancer Res 24:1427-32. 2004
    ..We also demonstrated that MSLHV-NT12 EDN induces apoptosis in KSIMM cells. In conclusion, MSLHV-NT12 EDN is a specific proapoptotic substance for KS cells, which warrants further investigation into its in vivo effects...
  13. ncbi Circadian variation of exhaled nitric oxide and urinary eosinophil protein X in asthmatic and healthy children
    Joerg Mattes
    University Children s Hospital Freiburg, D 79106 Freiburg, Germany
    Pediatr Res 51:190-4. 2002
    ..Rhythmicity of EPX excretion and FeNo in healthy children suggests a physiologic mechanism; however, pathologic effects during the night might occur under conditions of asthma-specific inflammation...
  14. pmc Sequence variation at two eosinophil-associated ribonuclease loci in humans
    J Zhang
    Laboratory of Host Defenses, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
    Genetics 156:1949-58. 2000
    ....
  15. ncbi Urine eosinophil protein X (EPX) is an in vitro parameter of inflammation in atopic dermatitis of the adult age
    K Breuer
    Department of Dermatology and Allergology, Hannover Medical University, Ricklinger Strasse 5, D-30449 Hannover, Germany
    Allergy 56:780-4. 2001
    ..36, P<0.0001 and 0.34, P<0.0001, respectively). CONCLUSION: Urine EPX is a useful in vitro parameter of inflammation in atopic dermatitis of the adult age...
  16. ncbi Structure and chromosome localization of the human eosinophil-derived neurotoxin and eosinophil cationic protein genes: evidence for intronless coding sequences in the ribonuclease gene superfamily
    K J Hamann
    Department of Immunology, Mayo Clinic and Foundation, Rochester, Minnesota 55905
    Genomics 7:535-46. 1990
    ..It is likely that these two genes arose as a consequence of a gene duplication event that took place approximately 25-40 million years ago and that a subset of anthropoid primates possess both of these genes or closely related genes...
  17. ncbi The effect of nasal polyp epithelial cells on eosinophil activation
    Seung Heon Shin
    Department of Otolarynology, School of Medicine, Catholic University of Daegu, South Korea
    Laryngoscope 113:1374-7. 2003
    ..The study was designed to investigate the effect of human nasal epithelial cells on the activation of eosinophils...
  18. ncbi Estradiol in premenstrual asthma: a double-blind, randomized, placebo-controlled, crossover study
    Mary H H Ensom
    Faculty of Pharmaceutical Sciences, University of British Columbia, Canada
    Pharmacotherapy 23:561-71. 2003
    ....
  19. ncbi Inhibitory effects of ketotifen on eotaxin-dependent activation of eosinophils: consequences for allergic eye diseases
    G Woerly
    Centre d Immunologie et Biologie Parasitaire, Unité INSERM IPL U547, Institut Pasteur, Lille, France
    Allergy 58:397-406. 2003
    ..The aim of this study was to investigate the effects of ketotifen on different parameters of human eosinophil functions, namely chemotaxis, oxidative metabolism and mediator release, induced after activation...
  20. ncbi Extended exhaled NO measurement differentiates between alveolar and bronchial inflammation
    L Lehtimäki
    Immunopharmacological Research Group, University of Tampere Medical School, Tampere, Finland
    Am J Respir Crit Care Med 163:1557-61. 2001
    ..In conclusion, extended exhaled NO measurement can be used to separately assess alveolar and bronchial inflammation and to assess disease activity/severity in asthma and alveolitis...
  21. ncbi Effect of physical and chemical methods of homogenization on inflammatory mediators in sputum of asthma patients
    E Grebski
    Pulmonary Division, Department of Internal Medicine, University of Zurich, Switzerland
    Chest 119:1521-5. 2001
    ..CONCLUSIONS: The sputum processing by DTT caused a statistically significant fall in EPO and MPO concentrations but did not significantly influence the measured concentrations of ECP and EPX...
  22. ncbi Eosinophil markers in blood, serum, and urine for monitoring the clinical course in childhood asthma: impact of budesonide treatment and withdrawal
    K Lönnkvist
    Astrid Lindgren Children s Hospital, Stockholm and the Department of Laboratory Medicine, Division of Clinical Immunology, Karolinska Hospital, Stockholm
    J Allergy Clin Immunol 107:812-7. 2001
    ..Few studies have focused on the dynamics of airway inflammation as reflected by the activity of the eosinophils and their proteins after withdrawal of inhaled corticosteroids...
  23. ncbi Both aromatic and cationic residues contribute to the membrane-lytic and bactericidal activity of eosinophil cationic protein
    Esther Carreras
    Departament de Bioquimica i Biologia Molecular, Facultat de Ciencies, Universitat Autonoma de Barcelona, 08193 Bellaterra, Spain
    Biochemistry 42:6636-44. 2003
    Eosinophil cationic protein (ECP) and eosinophil derived neurotoxin (EDN) are proteins of the ribonuclease A (RNase A) superfamily that have developed biological properties related to the function of eosinophils...
  24. ncbi Neuropathological findings in hypereosinophilic syndrome
    Hiroo Yoshikawa
    Intern Med 42:381-2. 2003
  25. ncbi Gene structure and enzymatic activity of mouse eosinophil-associated ribonuclease 2
    A L McDevitt
    Eosinophil Biology Unit, Laboratory of Host Defenses 10 11N104, NIAID National Institutes of Health, 9000 Rockville Pike, Bethesda, MD 20892 1886, USA
    Gene 267:23-30. 2001
    ..Sequence analysis of the non-coding regions and enzymatic characterization of the gene product provide further evidence indicating that mEAR-2 is a structural and functional ortholog of primate EDNs and ECPs...
  26. ncbi Ambient ozone exposure is associated with eosinophil activation in healthy children
    T Frischer
    University Children's Hospital Vienna, Austria
    Clin Exp Allergy 31:1213-9. 2001
    ..007 microg/mmol creatinine per ppb ozone; SE:0.02; P < 0.001). CONCLUSION: Our observation supports the hypothesis that ozone in healthy children is associated with eosinophil inflammation, most likely in the airways...
  27. ncbi Asthma attack severity and urinary concentration of eosinophil X protein in children
    Wassim Khalil Kalaajieh
    Public Health Faculty Lebanese University Najran Saudi Arabia
    Allergol Immunopathol (Madr) 30:225-31. 2002
    ..Determination of the urinary concentration of eosinophil protein X (U-EPX) may objectively predict the severity and activity of asthma in children...
  28. ncbi A new method for the quantification of neutrophil and eosinophil cationic proteins in feces: establishment of normal levels and clinical application in patients with inflammatory bowel disease
    Christer G B Peterson
    Pharmacia Diagnostics AB, Uppsala, Sweden
    Am J Gastroenterol 97:1755-62. 2002
    ....
  29. pmc The past as the key to the present: resurrection of ancient proteins from eosinophils
    Steven A Benner
    Department of Chemistry, University of Florida, Gainesville, FL 32611 7200, USA
    Proc Natl Acad Sci U S A 99:4760-1. 2002
  30. ncbi Eosinophil degranulation during pregnancy and after delivery by cesarean section
    Kenji Matsumoto
    Department of Allergy and Immunology, National Research Institute for Child Health and Development, Tokyo, Japan
    Int Arch Allergy Immunol 131:34-9. 2003
    ....
  31. pmc Localized eosinophil degranulation mediates disease in tropical pulmonary eosinophilia
    Laura O'Bryan
    National Institutes of Health, Bethesda, Maryland, USA
    Infect Immun 71:1337-42. 2003
    ..02). These data suggest it is the eosinophilic granular protein EDN, an RNase capable of damaging the lung epithelium, that plays the most important role in the pathogenesis of TPE...
  32. ncbi Circadian variations in serum eosinophil cationic protein, and serum and urine eosinophil protein X
    O D Wolthers
    Children s Clinic Randers, Randers, Department of Paediatrics, Denmark
    Pediatr Allergy Immunol 14:130-3. 2003
    ..To avoid confounding influence from circadian variations in ECP and EPX in clinical studies blood or urine should be sampled at consistent times...
  33. ncbi Serum eosinophil-derived neurotoxin (EDN) in diagnosis and evaluation of severity and bronchial hyperresponsiveness in childhood asthma
    Kyung Won Kim
    Department of Pediatrics and Institute of Allergy, Brain Korea 21 Project for Medical Sciences, Biomolecule Secretion Research Center, Yonsei University College of Medicine, Seoul, Korea
    Lung 185:97-103. 2007
    ....
  34. ncbi Inhaled fluticasone decreases bronchial but not alveolar nitric oxide output in asthma
    L Lehtimäki
    The Immunopharmacological Research Group, Medical School, University of Tampere, Dept of Clinical Chemistry, Finland
    Eur Respir J 18:635-9. 2001
    ..In conclusion, inhaled fluticasone decreases bronchial but not alveolar nitric oxide output simultaneously with clinical improvement in patients with asthma...
  35. ncbi Increased urinary leukotriene E4 and eosinophil protein X excretion in patients with interstitial cystitis
    K Bouchelouche
    Smooth Muscle Laboratory, Departments of Urology and Pathology, Herlev Hospital, University of Copenhagen, Herlev, Pharmacia Upjohn, Copenhagen, Denmark
    J Urol 166:2121-5. 2001
    ..However, it remains to be investigated whether the increase in urinary leukotriene E4 and eosinophil protein X correlates with interstitial cystitis symptoms...
  36. ncbi Comparison of the effects of repetitive low-dose and single-dose antigen challenge on airway inflammation
    Lin Ying Liu
    Allergy and Immunology Section of the Department of Medicine, University of Wisconsin Hospital, Madison, USA
    J Allergy Clin Immunol 111:818-25. 2003
    ..Allergen provocation is usually administered as a relatively large single-dose challenge that might not reflect a chronic, natural, low-dose airborne allergen exposure...
  37. ncbi Urinary leukotriene E(4), eosinophil protein X, and nasal eosinophil cationic protein are not associated with respiratory symptoms in 1-year-old children
    C Wojnarowski
    Wilhelminenspital, Department of Pediatric Infectious Diseases, Vienna, Austria
    Allergy 56:883-8. 2001
    ..With regard to respiratory symptoms, urinary and nasal inflammatory parameters are not helpful in characterizing the phenotype of a single patient...
  38. ncbi The effect of cationic charge on release of eosinophil mediators
    Darryl J Adamko
    Department of Pediatrics, Pulmonary Research Group, University of Alberta, Edmonton, Alberta, Canada
    J Allergy Clin Immunol 122:383-90, 390.e1-4. 2008
    ..Although the role of cytokines in cell activation is well established, the presence of cationic-charged tissue can also be an important factor in inflammatory cell function...
  39. ncbi Evidence for glycosylphosphatidylinositol (GPI)-anchored eosinophil-derived neurotoxin (EDN) on human granulocytes
    Françoise Debierre-Grockiego
    Institute for Virology, Medical Center for Hygiene, Philipps University, D 35037 Marburg, Germany
    FEBS Lett 537:111-6. 2003
    ..Furthermore, metabolic labeling with GPI anchor components supports biochemical evidence of GPI anchoring of EDN...
  40. ncbi Refined crystal structures of native human angiogenin and two active site variants: implications for the unique functional properties of an enzyme involved in neovascularisation during tumour growth
    D D Leonidas
    Department of Biology and Biochemistry, University of Bath, Claverton Down, BA2 7AY, UK
    J Mol Biol 285:1209-33. 1999
    ..The H13A structure shows some changes beyond the ribonucleolytic site, but sites involved in cell-binding and nuclear translocation are essentially unaffected by the amino acid replacement...
  41. ncbi Efficacy of a short course of parent-initiated oral prednisolone for viral wheeze in children aged 1-5 years: randomised controlled trial
    Abraham Oommen
    Leicester Children s Asthma Centre, Institute for Lung Health, University of Leicester, Leicester, UK
    Lancet 362:1433-8. 2003
    ..However, evidence for this treatment strategy is conflicting. We therefore aimed to assess the efficacy of a short course of oral prednisolone for preschool viral wheeze, with stratification for systemic eosinophil priming...
  42. ncbi Differential extraction of eosinophil granule proteins
    Lyo E Ohnuki
    Department of Dermatology, University of Utah, Salt Lake City, 84132, USA
    J Immunol Methods 307:54-61. 2005
    ..These results indicate that repetitive extraction of eosinophil granules yields an increased amount of all granule proteins, and that MBP2 can now be recovered in good quantities and in a relatively pure form...
  43. ncbi Inflammatory changes associated with circadian variation in pulmonary function in subjects with mild asthma
    E A B Kelly
    Pulmonary and Critical Care Medicine Section, Department of Medicine, University of Wisconsin, Madison, WI 53792, USA
    Clin Exp Allergy 34:227-33. 2004
    ..Nocturnal enhancement of airway inflammation has been demonstrated in patients with asthma who have a significant drop in pulmonary function at night...
  44. ncbi Familial eosinophilia: a benign disorder?
    Amy D Klion
    National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Blood 103:4050-5. 2004
    ..These data suggest that, despite prolonged marked eosinophilia, FE can be distinguished from HES by a more benign clinical course that may be related to a relative lack of eosinophil activation...
  45. ncbi [Effect of two doses of budesonide on exhaled nitric oxide and urinary EPX excretion in asthmatic children]
    K Storm van's Gravesande
    Zentrum für Kinderheilkunde und Jugendmedizin, Universitatsklinikum Freiburg
    Pneumologie 58:483-8. 2004
    ....
  46. pmc Differential effects of non-selective and selective phosphodiesterase inhibitors on human eosinophil functions
    A Hatzelmann
    Byk Gulden, Department of Biochemistry, Konstanz, Germany
    Br J Pharmacol 114:821-31. 1995
    ....
  47. ncbi CD28 and secretory immunoglobulin A-dependent activation of eosinophils: inhibition of mediator release by the anti-allergic drug, suplatast tosilate
    G Woerly
    Inserm U547 IFR17, Institut Pasteur de Lille, Lille, France
    Clin Exp Allergy 34:1379-87. 2004
    ....
  48. ncbi Identification of a purine-rich intronic enhancer element in the mouse eosinophil-associated ribonuclease 2 (mEar 2) gene
    Kimberly D Dyer
    Eosinophil Pathophysiology Section, LHD, NIAID, National Institutes of Health, Bethesda, Maryland 20892, USA
    Mamm Genome 15:126-34. 2004
    ..Similar intronic enhancers have been described as regulating transcription of the human EDN gene, suggesting an overall conservation of an important regulatory strategy...
  49. ncbi Peripheral blood eosinophils from patients with allergic asthma contain increased intracellular eosinophil-derived neurotoxin
    Julie B Sedgwick
    Allergy and Immunology, Department of Medicine, University of Wisconsin, Madison, WI 53792, USA
    J Allergy Clin Immunol 114:568-74. 2004
    ....
  50. ncbi Localization of the human eosinophil Charcot-Leyden crystal protein (lysophospholipase) gene (CLC) to chromosome 19 and the human ribonuclease 2 (eosinophil-derived neurotoxin) and ribonuclease 3 (eosinophil cationic protein) genes (RNS2 and RNS3) to chro
    D M Mastrianni
    Division of Hematology Oncology, Beth Israel Hospital, Boston, Massachusetts
    Genomics 13:240-2. 1992
  51. ncbi Serial blood and urine levels of EDN and ECP in eosinophilic colitis
    H Inamura
    Gunma Clinical Research Center for Allergy and Regeneration, Showa Hospital, 1341 Watanuki cho, Takasaki 370 1207, Japan
    Allergy 58:959-60. 2003
  52. ncbi Eosinophil granule proteins in peripheral blood granulocytes
    R I Abu-Ghazaleh
    Department of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, Minnesota 55905
    J Leukoc Biol 52:611-8. 1992
    ..5; EDN, 72 +/- 9; and ECP, 50 +/- 12. Therefore we conclude that these proteins are mainly expressed in eosinophils, but that certain ones are present in basophils and neutrophils...
  53. pmc High-resolution crystal structures of ribonuclease A complexed with adenylic and uridylic nucleotide inhibitors. Implications for structure-based design of ribonucleolytic inhibitors
    Demetres D Leonidas
    Institute of Organic and Pharmaceutical Chemistry, The National Hellenic Research Foundation, 11635 Athens, Greece
    Protein Sci 12:2559-74. 2003
    ..These inhibitors could be developed to therapeutic agents that could control the biological activities of EDN, ECP, and ANG, which play key roles in human pathologies...
  54. ncbi A kinetic study in adults with food hypersensitivity assessed as eosinophil activation in fecal samples
    J Magnusson
    Asthma and Allergy Research Group, Department of Respiratory Medicine, Sahlgrenska University Hospital, Gothenburg, Sweden
    Clin Exp Allergy 33:1052-9. 2003
    ..Immune-mediated food hypersensitivity affecting the gut is difficult to evaluate, and objective tools to diagnose local gastrointestinal (GI) inflammatory reactions are lacking...
  55. ncbi The stimulus-dependent release of eosinophil cationic protein and eosinophil protein x increases in apoptotic eosinophils
    K Seton
    Department of Medical Sciences, Unit of Clinical Chemistry, University Hospital, Uppsala University, Sweden
    Scand J Immunol 58:312-20. 2003
    ..Our results suggest that apoptotic eosinophils are potentially harmful cells that have retained their ability to react to certain extracellular stimuli. The findings point to unexpected consequences of eosinophil apoptosis...
  56. ncbi Identification of local Th2 and Th0 lymphocytes in vernal conjunctivitis by cytokine flow cytometry
    A Leonardi
    Department of Physiopathologic Optics, Institute of Ophthalmology, University of Padua, Italy
    Invest Ophthalmol Vis Sci 40:3036-40. 1999
    ..Cytokine flow cytometry of tear samples was used to identify the phenotypical and functional properties of lymphocytes at the actual site of the allergic reaction...
  57. ncbi Seasonal differences of peak expiratory flow rate variability and mediators of allergic inflammation in non-atopic adolescents
    H A Ferdousi
    Department of Paediatrics, University Hospital of Linkoping, Linkoping, Sweden
    Pediatr Allergy Immunol 12:238-46. 2001
    ..Normal limits for mediators were higher and PEF variability was almost the same as has been reported in adults. When using normal values, seasonal influences should be considered...
  58. ncbi Urinary eosinophil protein X in children with asthma: influence of atopy and airway infections
    K Oymar
    Department of Pediatrics, Rogaland Central Hospital, Stavanger, Norway
    Pediatr Allergy Immunol 12:34-41. 2001
    ....
  59. ncbi Eosinophil cationic protein cDNA. Comparison with other toxic cationic proteins and ribonucleases
    R L Barker
    Department of Immunology, Mayo Medical School, Rochester, MN 55905
    J Immunol 143:952-5. 1989
    ..These data suggest that ECP and EDN are derived from a common ancestral ribonuclease gene and that ECP has evolved into a potent helminthotoxin similar in some respects to MBP, while losing much of its ribonuclease activity...
  60. ncbi Increased production of cysteinyl leukotrienes and prostaglandin D2 during human anaphylaxis
    E Ono
    Clinical Research Center for Allergy and Rheumatology, National Hospital Organization, Sagamihara National Hospital, Sagamihara, Kanagawa, Japan
    Clin Exp Allergy 39:72-80. 2009
    ..However, pathological evidence of the association between inflammatory mediators and human anaphylaxis is insufficient...
  61. pmc Human eosinophils recognize endogenous danger signal crystalline uric acid and produce proinflammatory cytokines mediated by autocrine ATP
    Takehito Kobayashi
    Division of Allergic Diseases, Department of Medicine and Department of Immunology, Mayo Clinic, Rochester, MN 55905, USA
    J Immunol 184:6350-8. 2010
    ..These responses by eosinophils to tissue damage may explain the self-perpetuating nature of chronic inflammation in certain human diseases, such as asthma...
  62. ncbi Eosinophils induce DC maturation, regulating immunity
    Ramin Lotfi
    Surgery and Bioengineering, Hillmann Cancer Center, 5117 Centre Avenue, Pittsburgh, PA 15213, USA
    J Leukoc Biol 83:456-60. 2008
    ..The role of viral or bacterial products or potentially, host-derived DNA as eosinophil activators with consequent DC maturation should be considered in more detail in the inflammatory settings in which eosinophils have been observed...
  63. pmc Perturbations in eosinophil homeostasis following treatment of lymphatic filariasis
    R Gopinath
    Helminth Immunology Section, Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
    Infect Immun 68:93-9. 2000
    ....
  64. ncbi Purification and characterization of a ribonuclease from human liver
    S Sorrentino
    Department of Biological Chemistry, UCLA School of Medicine 90024 1737
    J Biol Chem 263:16125-31. 1988
    ..Cross-reactive ribonuclease is present in serum, but levels are not correlated with any of the disease states examined...
  65. pmc Protein microarray analysis in patients with asthma: elevation of the chemokine PARC/CCL18 in sputum
    Hyo Bin Kim
    Department of Pediatrics, Asthma and Allergy Center, Inje University Sanggye Paik Hospital, Seoul, Korea
    Chest 135:295-302. 2009
    ..To identify novel factors produced in the asthmatic airway, we analyzed sputum samples by using a membrane-based human cytokine microarray technology in patients with bronchial asthma (BA)...
  66. ncbi Eosinophils alter colonic epithelial barrier function: role for major basic protein
    Glenn T Furuta
    Combined Program in Pediatric Gastroenterology and Nutrition, Harvard Medical School, Boston, MA 02115, USA
    Am J Physiol Gastrointest Liver Physiol 289:G890-7. 2005
    ..In conclusion, MBP decreases epithelial barrier function and in this manner contributes to the pathogenesis of inflammatory bowel diseases...
  67. ncbi Amino acid sequence of the nonsecretory ribonuclease of human urine
    J J Beintema
    Biochemisch Laboratorium, Rijksuniversiteit, Groningen, The Netherlands
    Biochemistry 27:4530-8. 1988
    ..The nonsecretory ribonuclease has five short carbohydrate chains attached via asparagine residues at the surface of the molecule; these chains may have been shortened by exoglycosidase action.(ABSTRACT TRUNCATED AT 250 WORDS)..
  68. ncbi Eosinophils function as antigen-presenting cells
    Huan Zhong Shi
    First Affiliated Hospital, Guangxi Medical University, Nanning 530021, Guangxi, P R China
    J Leukoc Biol 76:520-7. 2004
    ..Furthermore, eosinophils within the lumina of airways promote expansion of T helper cell type 2 (Th2) by presenting antigen, suggesting that eosinophils actively modulate immune responses by amplifying Th2 cell responses...
  69. ncbi Rapid evolution of the ribonuclease A superfamily: adaptive expansion of independent gene clusters in rats and mice
    N A Singhania
    Laboratory of Host Defenses, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Building 10 Bethesda, MD 20892, USA
    J Mol Evol 49:721-8. 1999
    ....
  70. ncbi The kinetics of allergen-induced eotaxin level in nasal lavage fluid: its key role in eosinophil recruitment in nasal mucosa
    N Terada
    Department of Otorhinolaryngology, Chiba University School of Medicine, Chiba, Japan
    Am J Respir Crit Care Med 164:575-9. 2001
    ..These results indicate that eotaxin has an important role in eosinophil-dependent inflammation in nasal mucosa and suggest that blocking eotaxin or CCR-3 might be useful for new therapeutic tools of allergic rhinitis...
  71. ncbi Synergistic effect of theophylline and procaterol on interleukin-5-induced degranulation from human eosinophils
    Takao Fujisawa
    Department of Pediatrics and Allergy, National Mie Hospital, Tsu City, Japan
    J Asthma 39:21-7. 2002
    ..The inhibition was comparable to that with dexamethasone at 10(-9) M. Our results suggest that a combination of low-dose theophylline and procaterol exhibits antiinflammatory effects in asthma by inhibiting eosinophil-effector functions...
  72. ncbi Detection of local mast-cell activity in patients with food hypersensitivity
    C G B Peterson
    Department of Medical Sciences, Clinical Chemistry, Uppsala University, Uppsala, Sweden
    J Investig Allergol Clin Immunol 17:314-20. 2007
    ..However, to date, assessment of mast-cell activation using serum markers has been unsuccessful...
  73. ncbi Leukocytes and mediators in bronchoalveolar lavage during allergen-induced late-phase asthmatic reactions
    P Diaz
    Department of Experimental Medicine, University of Chile, Santiago
    Am Rev Respir Dis 139:1383-9. 1989
    ..05) in LPR, but not in SER. These findings support the hypothesis that eosinophils and their products play a role in tissue injury in LPR and that eosinophil infiltration may be associated with macrophage activation...
  74. ncbi Association of transient dermal mastocytosis and elevated plasma tryptase levels with development of adverse reactions after treatment of onchocerciasis with ivermectin
    Philip J Cooper
    Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA
    J Infect Dis 186:1307-13. 2002
    ..The data indicate that mast cells play a role in initiation of tissue inflammatory reactions after ivermectin treatment of onchocerciasis...
  75. ncbi Crystal structure of eosinophil cationic protein at 2.4 A resolution
    E Boix
    Department of Biology and Biochemistry, University of Bath, UK
    Biochemistry 38:16794-801. 1999
    ..The structure also shows how the cationic residues are distributed on the surface of the ECP molecule that may have implications for an understanding of the cytotoxicity of this enzyme...
  76. ncbi Prospective longitudinal study of urinary eosinophil protein X in children with asthma and chronic cough
    A Labbe
    Service de Pédiatrie A, Hotel Dieu, Clermont Ferrand, France
    Pediatr Pulmonol 31:354-62. 2001
    ..02). In contrast, there was no significant change in PBE levels. Urinary EPX concentrations are sensitive, noninvasive technique that could be useful to the clinician in the evaluation of manifestations of airway inflammation...
  77. ncbi A functional study on CysLT(1) receptors in human eosinophils
    Nobuharu Ohshima
    Department of Respiratory Medicine, University of Tokyo Graduate School of Medicine, Tokyo, Japan
    Int Arch Allergy Immunol 129:67-75. 2002
    ..The cysteinyl leukotrienes (CysLTs) mediate their biological actions through two receptors: CysLT(1) receptor and CysLT(2) receptor...
  78. ncbi A promising alternative anti-HBV agent: the targeted ribonuclease
    Yinghui Li
    Department of Medical Microbiology and Parasitology, Fourth Military Medical University, Xi an, Shaanxi 710032, PR China
    Int J Mol Med 26:51-6. 2010
    ..HBV may therefore be particularly vulnerable to the capsid-targeted viral inactivation approach...
  79. pmc Innate antifungal immunity of human eosinophils mediated by a beta 2 integrin, CD11b
    Juhan Yoon
    Department of Immunology, Division of Allergic Disease, Mayo Graduate School, Mayo Clinic, Rochester, MN 55905, USA
    J Immunol 181:2907-15. 2008
    ..This immune response by eosinophils may benefit the host, but, in turn, it may also play a role in the development and/or exacerbation of eosinophil-related allergic human diseases, such as asthma...
  80. ncbi Increased urinary leukotriene E4 concentration in patients with eosinophilic pneumonia
    E Ono
    Clinical Research Centre for Allergy and Rheumatology, Sagamihara National Hospital, Sakuradai 18 1, Sagamihara, Kanagawa 228 8522, Japan
    Eur Respir J 32:437-42. 2008
    ....
  81. ncbi Treatment of chronic rhinosinusitis with intranasal amphotericin B: a randomized, placebo-controlled, double-blind pilot trial
    Jens U Ponikau
    Department of Otorhinolaryngology Head and Neck Surgery, Mayo Clinic, Rochester, MN 55905, USA
    J Allergy Clin Immunol 115:125-31. 2005
    ..Chronic rhinosinusitis (CRS) is one of the most common chronic diseases. Its etiology is unknown, and there is a paucity of effective medical treatments...
  82. ncbi Crystal structures of ribonuclease A complexes with 5'-diphosphoadenosine 3'-phosphate and 5'-diphosphoadenosine 2'-phosphate at 1.7 A resolution
    D D Leonidas
    School of Biology and Biochemistry, University of Bath, Claverton Down, U K
    Biochemistry 36:5578-88. 1997
    ....
  83. ncbi The crystal structure of eosinophil cationic protein in complex with 2',5'-ADP at 2.0 A resolution reveals the details of the ribonucleolytic active site
    C Gopi Mohan
    Department of Biology and Biochemistry, University of Bath, Claverton Down, Bath BA2 7AY, U K
    Biochemistry 41:12100-6. 2002
    ....
  84. ncbi Weaning to hypoallergenic formula improves gut barrier function in breast-fed infants with atopic eczema
    Taina Arvola
    Paediatric Research Centre, University of Tampere and Department of Paediatrics, Tampere University Hospital, Finland
    J Pediatr Gastroenterol Nutr 38:92-6. 2004
    ....
  85. ncbi Emotional and behavioural problems in Swedish 7- to 9-year olds with asthma
    K Reichenberg
    The Nordic School of Public Health, PO Box 12133, SE 402 42 Goteborg, Sweden
    Chron Respir Dis 1:183-9. 2004
    ..The purpose of this study is to compare emotional and behavioural problems between preadolescent children with asthma and healthy children, and to explore if disease factors relate to problem scores...
  86. pmc Molecular signature of clinical severity in recovering patients with severe acute respiratory syndrome coronavirus (SARS-CoV)
    Yun Shien Lee
    Genomic Medicine Research Core Laboratory, Chang Gung Memorial Hospital, Tao Yuan, Taiwan
    BMC Genomics 6:132. 2005
    ..6% mortality rate. Although immunopathological damages may account for the severity of respiratory distress, little is known about how the genome-wide gene expression of the host changes under the attack of SARS-CoV...
  87. ncbi The human eosinophil proteome. Changes induced by birch pollen allergy
    Charlotte Woschnagg
    Department of Medical Sciences, Clinical Chemistry, Uppsala University, 75185 Uppsala, Sweden
    J Proteome Res 8:2720-32. 2009
    ..The link between these findings and an increased cellular antigen-presenting capacity and motility are discussed...
  88. ncbi Human eosinophils exert TNF-α and granzyme A-mediated tumoricidal activity toward colon carcinoma cells
    Fanny Legrand
    Inserm Unité 547, Institut Fédératif de Recherche 142, Lille, France
    J Immunol 185:7443-51. 2010
    ....
  89. ncbi The antipathogen activities of eosinophil cationic protein
    Ester Boix
    Departament Bioquimica i Biologia Molecular, Facultat Biociències, Universitat Autonoma de Barcelona, 08193 Cerdanyola del Valles, Spain
    Curr Pharm Biotechnol 9:141-52. 2008
    ..antibacterial activity, which is not shared by the other, closely-related eosinophil ribonuclease, the eosinophil derived neurotoxin (EDN)...
  90. pmc RNase A ribonucleases and host defense: an evolving story
    Helene F Rosenberg
    Laboratory of Allergic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 9000 Rockville Pike, Bethesda, MD 20892, USA
    J Leukoc Biol 83:1079-87. 2008
    ..The properties of individual RNase A ribonucleases, recent experimental findings, and important questions for the near and distant future will be reviewed...
  91. ncbi Possible release of eosinophil granule proteins in response to signaling from intercellular adhesion molecule-1 and its ligands
    J Chihara
    Fourth Department of Internal Medicine, Kinki University School of Medicine, Osaka, Japan
    Int Arch Allergy Immunol 108:52-4. 1995
    ..g. eosinophil cationic protein and eosinophil-derived neurotoxin...
  92. pmc Post-translational tyrosine nitration of eosinophil granule toxins mediated by eosinophil peroxidase
    Martina Ulrich
    Institute of Medical Microbiology and Hygiene, Universitatsklinikum Tubingen, Tübingen 72074, Germany
    J Biol Chem 283:28629-40. 2008
    ..Tyrosine nitration of eosinophil granule toxins occurs during maturation of eosinophils, independent of inflammation. These results provide evidence that post-translational tyrosine nitration is unique to eosinophils...
  93. ncbi The role of TNF-alpha in eosinophilic inflammation associated with RSV bronchiolitis
    Jungi Choi
    Department of Pediatrics and Inje University Sanggye Paik Hospital, Seoul, Korea
    Pediatr Allergy Immunol 21:474-9. 2010
    ..0001), IFN-gamma (r = 0.92, p < 0.0001), eotaxin (r = 0.64, p < 0.0001), and IL-8 (r = 0.84, p < 0.0001). TNF-alpha may have an important role in eosinophilic inflammation of airways in children with RSV bronchiolitis...
  94. ncbi Identification and characterization of human eosinophil cationic protein by an epitope-specific antibody
    E Boix
    Departament de Bioquimica i Biologia Molecular, Facultat de Ciencies, Universitat Autonoma de Barcelona, 08193 Bellaterra, Spain
    J Leukoc Biol 69:1027-35. 2001
    ....
  95. ncbi CpG oligodeoxynucleotide prolongs eosinophil survival through activation of contaminating B cells and plasmacytoid dendritic cells in vitro
    Kenji Matsumoto
    Department of Allergy and Immunology, National Research Institute for Child Health and Development, Tokyo, Japan
    Int Arch Allergy Immunol 140:42-50. 2006
    ....
  96. ncbi Short-term growth in children with eczema during treatment with topical mometasone furoate and tacrolimus
    Josefine Gradman
    Children s Clinic Randers, Randers, Denmark
    Acta Paediatr 96:1233-7. 2007
    ..Treatment with topical glucocorticoids in children with atopic eczema may be associated with systemic adverse effects, such as suppression of growth...
  97. ncbi Eosinophil-derived neurotoxin in childhood asthma: correlation with disease severity
    Chang Keun Kim
    Asthma and Allergy Center, Department of Pediatrics, Inje University Sanggye Paik Hospital, Seoul, Korea
    J Asthma 47:568-73. 2010
    ....
  98. ncbi Eosinophil sequestration and activation are associated with the onset and severity of systemic adverse reactions following the treatment of onchocerciasis with ivermectin
    P J Cooper
    Laboratory of Parasitic Diseases, NIAID, National Institutes of Health, Bethesda, MD 20892 0425, USA
    J Infect Dis 179:738-42. 1999
    ..These results suggest that eosinophil sequestration and activation/degranulation are associated with the initiation and severity of ivermectin-associated adverse reactions...
  99. ncbi U-EPX levels and wheezing in infants and young children with and without RSV bronchiolitis
    Sigurdur Kristjansson
    Department of Pediatrics, Landspitali University Hospital, IS 101 Reykjavik, Iceland
    Respir Med 100:878-83. 2006
    ..In conclusion, RSV bronchiolitis severe enough to require in-ward care produces a significant, but transient increase in U-EPX. Furthermore, a high U-EPX at baseline appears to be associated with an increased risk of future wheezing...
  100. ncbi Eosinophils from hematopoietic stem cell recipients suppress allogeneic T cell proliferation
    Jennie Andersson
    Department of Infectious Diseases, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden Electronic address
    Biol Blood Marrow Transplant 20:1891-8. 2014
    ..To conclude, eosinophils from HSC transplant recipients and healthy subjects have a T cell suppressive capacity. ..
  101. ncbi Towards the rational design of antimicrobial proteins: single point mutations can switch on bactericidal and agglutinating activities on the RNase A superfamily lineage
    David Pulido
    Department of Biochemistry and Molecular Biology, Universitat Autonoma de Barcelona, Cerdanyola del Valles, Spain
    FEBS J 280:5841-52. 2013
    ..This study provides an example of how structure-guided protein engineering can successfully reproduce an evolution selection process towards the emergence of new physiological roles...

Research Grants11

  1. Alternaria and ribonucleases in Th2-type immunity
    Hirohito Kita; Fiscal Year: 2013
    ....
  2. ANTIVIRAL EFFECTS OF EOSINOPHILS IN THE LUNGS
    David Jacoby; Fiscal Year: 2007
    ..Eosinophil ribonucleases (eosinophil derived neurotoxin (EDN) and eosinophil cationic protein (ECP)) may also participate...
  3. Eosinophil Granule Proteins and Their Functions
    Gerald Gleich; Fiscal Year: 2006
    ..Overall, these studies will expand our knowledge of the molecules composing the eosinophil granule and provide new tools to dissect eosinophil function in disease. ..
  4. CARDIOTOXICITY OF EOSINOPHIL GRANULE CATIONIC PROTEINS
    Helene Rosenberg; Fiscal Year: 1991
    ..From an educational perspective, the planned program will provide the skills and experience necessary for the principal investigator to pursue a career as an independent clinician-scientist...
  5. NOVEL THERAPY FOR KAPOSI'S SARCOMA
    JAMES LARRICK; Fiscal Year: 2000
    ..PROPOSED COMMERCIAL APPLICATIONS: The market for an effective agent for Kaposi's sarcoma and other disorders of angiogenesis is substantial. ..
  6. Global Gene Expression in Chronic Rhinosinusitis
    DOUGLAS PLAGER; Fiscal Year: 2005
    ..These new molecular approaches should shed light on the mechanisms of CRS and open novel avenues for diagnosis and treatment of this difficult disease. ..
  7. Ventilator-Induced Lung Injury in Children
    Sabrina Heidemann; Fiscal Year: 2004
    ..The results of this study may serve as the basis for a multicenter clinical trial. ..
  8. HIV and Neutrophil Function in Oral Disease
    Larry Thomas; Fiscal Year: 2003
    ..As such, the results may provide an additional target to enhance innate immunity within the oral cavity and also to decrease the vertical transmission of HIV in breast milk. ..
  9. Neutrophils and HIV-1 infection
    Larry Thomas; Fiscal Year: 2006
    ..It is predicted that the results of this study will provide a basis for expanded and detailed analysis for potential and previously unrecognized involvement of neutrophils in HIV-1 transmission and disease. ..
  10. Urine Protein Biomarker Exploration in Ovarian Cancer
    Bin Ye; Fiscal Year: 2006
    ..This project, if successful, could fundamentally shift the approach to the early detection of ovarian cancer and significantly improve the survival rate of cases detected. [unreadable] [unreadable]..
  11. Evolution of vertebrate sensory genes
    Jianzhi Zhang; Fiscal Year: 2010
    ..These studies will also help understand human smell and taste variations and disorders. ..