- Concerted nitric oxide formation and release from the simultaneous reactions of nitrite with deoxy- and oxyhemoglobin
Vascular Medicine Branch, NHLBI, National Institutes of Health, Bethesda, Maryland 20892, USA
J Biol Chem 282:12916-27. 2007
..reacts with deoxyhemoglobin in an allosteric reaction that generates NO and oxidizes deoxyhemoglobin to methemoglobin. NO then reacts at a nearly diffusion-limited rate with deoxyhemoglobin to form iron-nitrosyl-hemoglobin, ..
- Methemoglobin--it's not just blue: a concise review
PPD Inc, Wilmington, North Carolina 28412, USA
Am J Hematol 82:134-44. 2007
..besides carrying oxygen to the tissues, and regulates vascular tone and inflammation via a redox couple with methemoglobin. Hemoglobin has iron in the reduced valance Fe(II) and methemoglobin has iron in the oxidized valance Fe (III),..
- Reduction of methemoglobin via electron transfer from photoreduced flavin: restoration of O2-binding of concentrated hemoglobin solution coencapsulated in phospholipid vesicles
Advanced Research Institute for Science and Engineering, Waseda University, Tokyo 169 8555, Japan
Bioconjug Chem 15:1037-45. 2004
Ferric methemoglobin is reduced to its ferrous form by photoirradiation either by direct photoexcitation of the heme portion to induce electron transfer from the surrounding media (Sakai at al...
- Pathway for heme uptake from human methemoglobin by the iron-regulated surface determinants system of Staphylococcus aureus
Department of Veterinary Molecular Biology, Montana State University, Bozeman, MT 59718, USA
J Biol Chem 283:18450-60. 2008
..of heme acquisition by this system, we have investigated hemin transfer between the various couples of human methemoglobin (metHb), IsdA, IsdB, IsdC, and IsdE by spectroscopic and kinetic analyses...
- Synthesis, antiprotozoal, antimicrobial, β-hematin inhibition, cytotoxicity and methemoglobin (MetHb) formation activities of bis(8-aminoquinolines)
Department of Medicinal Chemistry, National Institute of Pharmaceutical Education and Research, Sector 67, S A S Nagar, Punjab 160 062, India
Bioorg Med Chem 19:197-210. 2011
..antimicrobial (a panel of pathogenic bacteria and fungi), cytotoxicity, β-hematin inhibitory and methemoglobin (MetHb) formation activities...
- Shr of group A streptococcus is a new type of composite NEAT protein involved in sequestering haem from methaemoglobin
Department of Biology, College of Arts and Sciences, Georgia State University, Atlanta, GA 30303, USA
Mol Microbiol 78:739-56. 2010
..We suggest that Shr is the prototype of a new group of NEAT composite proteins involved in haem uptake found in pyogenic streptococci and Clostridium novyi...
- Functionally distinct NEAT (NEAr Transporter) domains within the Staphylococcus aureus IsdH/HarA protein extract heme from methemoglobin
Rosemarie M Pilpa
Department of Chemistry and Biochemistry, UCLA DOE Institute for Genomics and Proteomics, UCLA, Los Angeles, California 90095, USA
J Biol Chem 284:1166-76. 2009
..We also demonstrate that the IsdC NEAT domain can capture heme directly from Hb, suggesting that there are multiple pathways for heme transfer across the cell wall...
- MRI of thermally denatured blood: methemoglobin formation and relaxation effects
Department of Radiological Sciences, UCLA School of Medicine, Los Angeles, CA 90024 1721, USA
Magn Reson Imaging 17:1489-94. 1999
..The aims of this study were two-fold: to find evidence for heat-induced conversion of hemoglobin (Hb) to methemoglobin (mHb), and to investigate the effects of heat treatment of in-vitro blood components upon their MR relaxation ..
- Pro-stimulatory role of methemoglobin in inflammation through hemin oxidation and polymerization
Malaria Research Group, Department of Biotechnology, Indian Institute of Technology Guwahati, Assam 781039, India
Inflamm Allergy Drug Targets 12:68-78. 2013
..The current study aimed to characterize the role of major pro-oxidant factor methemoglobin present in the malaria culture supernatant contributing in inflammation during malaria...
- Plasma methemoglobin as a potential biomarker of anemic stress in humans
Gregory M T Hare
Department of Anesthesia, Keenan Research Centre in the Li Ka Shing Knowledge Institute, St Michael s Hospital, University of Toronto, 30 Bond Street, Toronto, ON, M5B 1W8, Canada
Can J Anaesth 59:348-56. 2012
..Hemoglobin (Hb) oxidation to methemoglobin (MetHb) may be a byproduct of such local NO production...
- Characterization of methemoglobin formation induced by 3,5-dichloroaniline, 4-amino-2,6-dichlorophenol and 3,5-dichlorophenylhydroxylamine
M A Valentovic
Department of Pharmacology, Marshall University School of Medicine, Huntington, WV 25704 9388, USA
Toxicology 118:23-36. 1997
..This study characterized the capacity of 3,5-dichloroaniline and two putative metabolites to induce methemoglobin formation. In vivo intraperitoneal (i.p.) administration of 0...
- EPR spectroscopy of nitrite complexes of methemoglobin
David E Schwab
Department of Chemistry and Biochemistry, Montana State University, P O Box 173400, Bozeman, Montana 59717, USA
Inorg Chem 49:6330-7. 2010
..raised in recent studies of the structure and affinity of the nitrite ligand in complexes with Fe(III) in methemoglobin (metHb)...
- Accuracy of methemoglobin detection by pulse CO-oximetry during hypoxia
John R Feiner
Department of Anesthesia and Perioperative Care, University of California at San Francisco, 521 Parnassus Ave, San Francisco, CA 94143 0648, USA
Anesth Analg 111:143-8. 2010
b>Methemoglobin in the blood cannot be detected by conventional pulse oximetry, although it can bias the oximeter's estimate (Spo2) of the true arterial functional oxygen saturation (Sao2)...
- Plasma levels of lidocaine, o-toluidine, and prilocaine after application of 8.5 g Oraqix in patients with generalized periodontitis: effect on blood methemoglobin and tolerability
Britt Marie Herdevall
Specialist Dental Clinic, Sodertalje, Sweden
Acta Odontol Scand 61:230-4. 2003
..5 g Oraqix (212.5 mg of lidocaine base and 212.5 mg of prilocaine base) in periodontal pockets was well tolerated and displayed a wide safety margin with respect to plasma levels normally associated with systemic toxicity...
- Alkyl substituted 2'-benzoylpyridine thiosemicarbazone chelators with potent and selective anti-neoplastic activity: novel ligands that limit methemoglobin formation
Iron Metabolism and Chelation Program, Department of Pathology, University of Sydney, Sydney, New South Wales 2006, Australia
J Med Chem 56:357-70. 2013
..Major findings include that methemoglobin formation mediated by the lipophilic t-BuBpT series was significantly (p < 0.05-0...
- Interference of patent blue dye with pulse oximetry readings, methemoglobin measurements, and blue urine in sentinel lymph node mapping: a case report and review of the literature
Hou Chuan Lai
Department of Anesthesiology, Tri Service General Hospital, National Defense Medical Center, Taipei, Taiwan
Acta Anaesthesiol Taiwan 49:162-4. 2011
..The unexpected events might be a consequence of excessive administration of PB dye. We also reviewed the published discourses in literature on the adverse effects of PB dye...
- Methemoglobin formation by triapine, di-2-pyridylketone-4,4-dimethyl-3-thiosemicarbazone (Dp44mT), and other anticancer thiosemicarbazones: identification of novel thiosemicarbazones and therapeutics that prevent this effect
Discipline of Pathology, University of Sydney, Sydney, New South Wales, Australia
Mol Pharmacol 82:105-14. 2012
..These studies have demonstrated that 3-AP induces methemoglobin (metHb) formation and hypoxia in patients, limiting its usefulness...
- Methemoglobin exposure produces toxicological effects in macrophages due to multiple ROS spike induced apoptosis
Malaria Research Group, Department of Biotechnology, Indian Institute of Technology, Guwahati, Assam 781039, India
Toxicol In Vitro 27:16-23. 2013
..Presence of methemoglobin in body fluids such as blood, cerebrospinal fluid and urine is associated with tissue damage...
- Methemoglobin incites primaquine toxicity through single-electron oxidation and modification
S N Balaji
Malaria Research Group, Department of Biotechnology, Indian Institute of Technology Guwahati, Guwahati, Assam, India
J Basic Clin Physiol Pharmacol 24:105-14. 2013
..Primaquine (Pq) metabolic products are responsible for drug-associated hemotoxicity and limit primaquine usage...
- Extracellular methemoglobin primes red blood cell aggregation in malaria: an in vitro mechanistic study
S N Balaji
Malaria Research Group, Department of Biotechnology, Indian Institute of Technology Guwahati, Guwahati 781039, Assam, India
FEBS Lett 587:350-7. 2013
..Ammonium sulfate fractionation and immunodepletion of methemoglobin from culture supernatant confirms methemoglobin as a major aggregant...
- The near-iron transporter (NEAT) domains of the anthrax hemophore IsdX2 require a critical glutamine to extract heme from methemoglobin
Erin S Honsa
Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, Texas 77030, USA
J Biol Chem 288:8479-90. 2013
..Additionally, we used a spectroscopic analysis to show that IsdX2 NEAT domains only scavenge heme from methemoglobin (metHb) and that autoxidation of oxyhemoglobin to metHb must occur prior to extraction...
- Reactions of deoxy-, oxy-, and methemoglobin with nitrogen monoxide. Mechanistic studies of the S-nitrosothiol formation under different mixing conditions
Laboratorium für Anorganische Chemie, Eidgenossische Technische Hochschule, ETH Honggerberg, CH 8093 Zurich, Switzerland
J Biol Chem 278:6623-34. 2003
..Our data strongly suggest that, because of mixing artifacts, care should be taken to use results from in vitro experiments to draw conclusion on the mechanism of the reaction in vivo...
- Effect of heavy metal exposure on blood haemoglobin concentration and methemoglobin percentage in Lumbricus terrestris
Department of Biological and Environmental Sciences and Technologies, University of Salento, via prov le Lecce Monteroni, 73100, Lecce, Italy
Ecotoxicology 20:847-54. 2011
..possible effect of heavy metal (cadmium, copper, mercury) exposure on Hb concentration and oxidation state (methemoglobin formation) in the earthworm Lumbricus terrestris...
- Metmyoglobin and methemoglobin catalyze the isomerization of peroxynitrite to nitrate
Laboratorium für Anorganische Chemie, Eidgenossische Technische Hochschule, ETH Honggerberg, CH 8093 Zurich, Switzerland
Biochemistry 42:14036-46. 2003
..Moreover, the globin offers very little protection, as the heme-free (apoMb) and heme-blocked (metMbCN) forms only partly prevent nitration of free tyrosine...
- Hemoglobin as a nitrite anhydrase: modeling methemoglobin-mediated N2O3 formation
Kathrin H Hopmann
Centre for Theoretical and Computational Chemistry and Department of Chemistry, University of Tromsø, N 9037 Tromsø, Norway
Chemistry 17:6348-58. 2011
..be readily explained by the reaction Hb-Fe(3+)+NO(2)(-)+NO⇌Hb-Fe(2+)+N(2)O(3), the exact manner in which methemoglobin (Hb-Fe(3+)), nitrite and NO interact with one another is unclear...
- An electron paramagnetic resonance study of the affinity of nitrite for methemoglobin
Bradley I Goetz
Department of Physics, Wake Forest University, Winston Salem, NC 27109, USA
Nitric Oxide 22:149-54. 2010
..The latter reaction is hypothesized to involve reactions on NO, nitrite and methemoglobin to form N(2)O(3) in an anhydrase reaction...
- Cerebral malaria and the hemolysis/methemoglobin/heme hypothesis: shedding new light on an old disease
Unidade de Malaria, Instituto de Medicina Molecular, Faculdade de Medicina da Universidade de Lisboa, 1649 028 Lisboa, Portugal
Int J Biochem Cell Biol 41:711-6. 2009
..In this review, we discuss the role of hemolysis, methemoglobin and free heme in CM, brought to light by our recent studies in mice as well as by other studies in humans.
- Consequences of methemoglobinemia in pregnancy in newborns, children, and adults: issues raised by new findings on methemoglobin catabolism
Department of Environmental Medicine, University of Rijeka School of Medicine, Rijeka, Croatia
J Matern Fetal Neonatal Med 23:956-9. 2010
The aim of this review is to warn about the effects of methemoglobin and its catabolic products and the toxic effects caused by environmental oxidants that cause high-risk pregnancy and may later impair the health of newborns, children ..
- Synthesis, antimalarial, antileishmanial, antimicrobial, cytotoxicity, and methemoglobin (MetHB) formation activities of new 8-quinolinamines
Department of Medicinal Chemistry, National Institute of Pharmaceutical Education and Research, Sector 67, S A S Nagar, Punjab 160 062, India
Bioorg Med Chem 15:915-30. 2007
..Several analogs, notably 21-24, 26-32, and 60, showed less MetHb formation compared to primaquine indicating the potential of these compounds in 8-quinolinamine-based antimalarial drug development...
- Blood levels of methemoglobin in patients with aluminum phosphide poisoning and its correlation with patient's outcome
Department of Medical Toxicology and Forensic Medicine, Loghman Hakim Poison Hospital, Shaheed Beheshti University of Medical Sciences, Tehran, Iran
J Med Toxicol 7:40-3. 2011
..following aluminum phosphide (AlP) intoxication has been reported, probable effect of blood level of methemoglobin (Met-Hb) on outcome of AlP-poisoned patients has not yet been investigated...
- The level of maternal methemoglobin during pregnancy in an air-polluted environment
Obstetric and Gynecologic Primary Care, Labin, Croatia
Environ Health Perspect 111:1902-5. 2003
..determine if a correlation could be established between the ground-level concentrations of sulfur dioxide and methemoglobin concentrations in pregnant women when a coal-powered thermoelectric power plant was in operation ("dirty" ..
- Simulation study of methemoglobin reduction in erythrocytes. Differential contributions of two pathways to tolerance to oxidative stress
Institute for Advanced Biosciences, Keio University, Fujisawa, Kanagawa 252 8520, Japan
FEBS J 274:1449-58. 2007
b>Methemoglobin (metHb), an oxidized form of hemoglobin, is unable to bind and carry oxygen. Erythrocytes are continuously subjected to oxidative stress and nitrite exposure, which results in the spontaneous formation of metHb...
- N-acetylcysteine reduces methemoglobin in vitro
R O Wright
Division of General Pediatrics, Children s Hospital, Boston, Massachusetts, USA
Ann Emerg Med 28:499-503. 1996
To determine whether N-acetylcysteine (NAC reduces methemoglobin.
- Discovery of a bulky 2-tert-butyl group containing primaquine analogue that exhibits potent blood-schizontocidal antimalarial activities and complete elimination of methemoglobin toxicity
National Institute of Pharmaceutical Education and Research, Sector 67, S A S Nagar, Punjab 160 062, India
J Med Chem 47:285-7. 2004
..yoelii nigeriensis strain in vivo. Compound 2 was also evaluated for methemoglobin (MetHb) toxicity...
- Internal electron transfer between hemes and Cu(II) bound at cysteine beta93 promotes methemoglobin reduction by carbon monoxide
Marine Freshwater Biomedical Center, Duke University Marine Laboratory, Beaufort, North Carolina 28516, USA
J Biol Chem 274:5499-507. 1999
..The beta hemes in Cu(II)-metHb are reduced under CO at rates close to those observed for cytochrome c oxidase, where heme and copper are present together in the oxygen-binding site and where internal electron transfer also occurs...
- Nitric oxide scavenging by barley hemoglobin is facilitated by a monodehydroascorbate reductase-mediated ascorbate reduction of methemoglobin
Abir U Igamberdiev
Department of Plant Science, University of Manitoba, Winnipeg, MB, R3T 2N2 Canada
Planta 223:1033-40. 2006
..NO scavenging is facilitated by monodehydroascorbate reductase mediating a coupled reaction involving ferric Hb reduction in the presence of ascorbate and NADH...
- Methemoglobin is a potent activator of endothelial cells by stimulating IL-6 and IL-8 production and E-selectin membrane expression
Department of Cell Biology and Molecular Medicine, UMDNJ New Jersey Medical School, 185 South Orange Avenue, MSB G 626, Newark, NJ 07103, USA
Am J Physiol Cell Physiol 285:C1036-46. 2003
..Endothelial cells are direct targets of free Hb or its oxidative derivatives, including methemoglobin (MHb) and hemin...
- Perioperative changes in carbonylhemoglobin and methemoglobin during abdominal surgery: alteration in endogenous generation of carbon monoxide
Department of Surgery, Kyoto City Hospital, Higashitakada cho, Mibu, Nakagyo ku, Japan
J Gastroenterol Hepatol 17:535-41. 2002
..Therefore, we investigated perioperative changes in CO generation during abdominal surgery...
- Trehalose glass-facilitated thermal reduction of metmyoglobin and methemoglobin
Department of Physiology and Biophysics, Albert Einstein College of Medicine, Bronx, New York 10461, USA
J Am Chem Soc 124:7270-1. 2002
- Characterization of erythrocytic uptake and release and disposition pathways of nitrite, nitrate, methemoglobin, and iron-nitrosyl hemoglobin in the human circulation
Yuen Yi Hon
Clinical Center Pharmacy Department, National Institutes of Health, 10 Center Drive, Bethesda, MD 20892, USA
Drug Metab Dispos 38:1707-13. 2010
..model to describe the in vivo erythrocytic uptake and release and disposition pathways of nitrite, nitrate, methemoglobin, and iron-nitrosyl hemoglobin in the human circulation...
- The role of methemoglobinemia in early and late complicated pregnancy
Obstetric and Gynecologic Primary Care, Creska 2, 52221 Rabac, Labin, Croatia
Med Hypotheses 68:1114-9. 2007
The objective of this review was to direct attention about methemoglobin as a biomarker which has an important role in the detection of adverse effects of the oxidative stress, misbalanced production of ROS, RNS and RSS...
- Measurement of carboxyhemoglobin and methemoglobin by pulse oximetry: a human volunteer study
Steven J Barker
Department of Anesthesiology, University of Arizona College of Medicine, P O Box 245114, Tucson, Arizona 85724 5114, USA
Anesthesiology 105:892-7. 2006
A new eight-wavelength pulse oximeter is designed to measure methemoglobin and carboxyhemoglobin, in addition to the usual measurements of hemoglobin oxygen saturation and pulse rate...
- Antioxidant protein 2 prevents methemoglobin formation in erythrocyte hemolysates
Karl M Stuhlmeier
Ludwig Boltzmann Institute for Rheumatology and Balneology, Vienna, Austria
Eur J Biochem 270:334-41. 2003
..Recombinant AOP2 prevented induced as well as noninduced methemoglobin formation in erythrocyte hemolysates, indicating its antioxidant properties...
- [The involvement of nitric oxide in formation of hemoglobin oxygen-binding properties]
V V Zinchuk
Grodno Medical University, Belarus
Usp Fiziol Nauk 34:33-45. 2003
..In reaction with hemoglobin NO forms methemoglobin, nitrosyl-hemoglobin (HbFe2+NO) and S-nitrosohemoglobin (SNO-Hb)...
- Hemoglobin autoxidation and regulation of endogenous H2O2 levels in erythrocytes
Robert M Johnson
Department of Biochemistry and Molecular Biology, Wayne State Medical School, 540 E Canfield, Detroit, MI 48201, USA
Free Radic Biol Med 39:1407-17. 2005
..b>Methemoglobin and the rate of catalase inactivation by 3-amino-2,4,5-triazole (3-AT) were determined...
- In vitro evaluation of antioxidant properties of Cocos nucifera Linn. water
Sudheer Kumar Mantena
Department of Pharmacology, Collega of Pharmaceutical Sciences, Manipal 576 199, Karnataka, India
Nahrung 47:126-31. 2003
..CW protected hemoglobin from nitrite-induced oxidation to methemoglobin when added before the autocatalytic stage of the oxidation...
- Vasoactivity of S-nitrosohemoglobin: role of oxygen, heme, and NO oxidation states
Jack H Crawford
Department of Pathology, University of Alabama at Birmingham, Birmingham, AL 35294, USA
Blood 101:4408-15. 2003
..This suggests that oxygen-dependent modulation of SNOHb vasoactivity does not occur by controlling the allosteric state of Hb but is a property of vessel responsiveness to nitrosovasodilators at low oxygen tensions...
- A 74-year-old woman with desaturation following surgery. Co-oximetry is the first step in making the diagnosis of dyshemoglobinemia
Matthias W König
Department of Anesthesiology, Wake Forest University School of Medicine, Winston Salem, NC 27157, USA
Chest 123:613-6. 2003
- Nitrite disrupts multiple physiological functions in aquatic animals
Frank B Jensen
Institute of Biology, University of Southern Denmark, Campusvej 55, DK 5230 M Odense, Denmark
Comp Biochem Physiol A Mol Integr Physiol 135:9-24. 2003
..Rainbow trout fall into two groups with regard to susceptibility and physiological response. These two groups are not related to sex but show significant different nitrite uptake rates...
- Blood viscosity maintains microvascular conditions during normovolemic anemia independent of blood oxygen-carrying capacity
La Jolla Bioengineering Institute, La Jolla, CA, USA
Am J Physiol Heart Circ Physiol 291:H581-90. 2006
Responses to exchange transfusion with red blood cells (RBCs) containing methemoglobin (MetRBC) were studied in an acute isovolemic hemodiluted hamster window chamber model to determine whether oxygen content participates in the ..
- Kinetics of the reactions of nitrogen monoxide and nitrite with ferryl hemoglobin
Laboratorium für Anorganische Chemie, Eidgenossische Technische Hochschule, ETH Honggerberg, Zurich, Switzerland
Free Radic Biol Med 34:531-45. 2003
..0 and 20 degrees C), but increases with decreasing pH (1.8 x 10(3) M(-1)s(-1) at pH 6.4 and 20 degrees C). Thus, under acidic conditions as found in ischemic tissues, this reaction may also have a physiological relevance...
- The effects of nitroglycerine on the redox status of rat erythrocytes and reticulocytes
S D Marković
Institute of Biology and Ecology, Faculty of Sciences, Kragujevac, Serbia
Physiol Res 55:389-96. 2006
..However, NTG robustly increased concentration of methemoglobin (MetHb), suggesting that NTG bio-transformation was primarily connected with hemoglobin (Hb)...
- Blood storage for forensic hemoglobin analysis using CO-oximeter
Hokkaido University Graduate School of Medicine, Sapporo 060 8638, Japan
Hokkaido Igaku Zasshi 78:289-95. 2003
..And changes in the values of oxyhemoglobin (O2Hb), methemoglobin (MetHb), carboxyhemoglobin (COHb), deoxyhemoglobin (HHb) and total hemoglobin (THb) were examined...
- Oxidation of hemoglobin to methemoglobin in intact erythrocyte by a hydroperoxide induces formation of glutathionyl hemoglobin and binding of alpha-hemoglobin to membrane
Department of Nutrition and Health Science, Faculty of Human Environmental Science, Fukuoka Women s University, 1 1 1 Kasumigaoka, Higashi ku, Fukuoka, Japan
Arch Biochem Biophys 417:244-50. 2003
..The formation of G-Hb occurred linearly until 10 min in parallel with the formation of methemoglobin (metHb) after exhaustion of reduced glutathione...
- Inhibitory effects of flavonoids on free radical-induced hemolysis and their oxidative effects on hemoglobin
Niigata University of Pharmacy and Applied Life Sciences, Faculty of Pharmaceutical Sciences, Niigata, Japan
Chem Pharm Bull (Tokyo) 52:999-1001. 2004
..of AAPH, the relatively hydrophobic flavonoids quercetin and morin induced the oxidation of oxyhemoglobin to methemoglobin. Oxidation by quercetin was especially marked. However, this oxidation did not induce hemolysis...
- Cross-linking with O-raffinose lowers oxygen affinity and stabilizes haemoglobin in a non-cooperative T-state conformation
Laboratory of Biochemistry and Vascular Biology, Division of Hematology, Center for Biologics Evaluation and Research CBER, Food and Drug Administration FDA, Bethesda, Maryland 20892, USA
Biochem J 384:367-75. 2004
..Although the physiological ramifications of locking HbA(0) in the T conformation with the O-raffinose are still unknown, valuable insights into haemoglobin function are provided by these studies of O-R-polyHbA(0)...
- Metabolism of pyrogallol to purpurogallin by human erythrocytic hemoglobin
Department of Geriatric Medicine, Tokyo Medical University, Japan
Tohoku J Exp Med 203:319-30. 2004
..having three hydroxy groups at the adjacent positions in the benzene ring, oxidized human oxyhemoglobin to methemoglobin and reduced human methemoglobin to oxyhemoglobin...
- Scavenging of reactive nitrogen species by oxygenated hemoglobin: globin radicals and nitrotyrosines distinguish nitrite from nitric oxide reaction
Department of Cell Biology and Neuroscience, Istituto Superiore di Sanita, Rome, Italy
Free Radic Biol Med 37:1244-55. 2004
..Collectively, these results indicate that NO2- reaction with oxyHb causes an oxidative challenge not observed with *NO. The differences in oxidation mechanisms of *NO and NO2- are discussed...
- Laboratory assessment of oxygenation in methemoglobinemia
Department of Pathology and Immunology, Washington University School of Medicine, 660 South Euclid Ave, St Louis, MO 63110, USA
Clin Chem 51:434-44. 2005
..This case conference reviews laboratory methods for assessing oxygenation status: arterial blood gases, pulse oximetry, and CO-oximetry. Caveats of these measurements are discussed in the context of two methemoglobinemia cases...
- Simultaneous monitoring of nitric oxide, oxyhemoglobin and deoxyhemoglobin from small areas of the rat brain by in vivo visible spectroscopy and a least-square approach
Felipe A Martín
Area de Psicobiologia, Universidad de La Laguna, Tenerife 38201, Canary Islands, Spain
J Neurosci Methods 140:75-80. 2004
..Under physiological conditions, NO reacts with oxyhemoglobin (OxyHb) to form methemoglobin (MetHb) at a very high rate...
- Small angle x-ray scattering (SAXS) study of the extracellular Hemoglobin of Glossoscolex paulistus: effect of pH, iron oxidation state, and interaction with anionic SDS surfactant
Emerson Luiz Gelamo
Departamento de Quimica e Fisica Molecular, Instituto de Quimica de Sao Carlos, Universidade de Sao Paulo, USP, C P 780, 13560 970 Sao Carlos, Sao Paulo, Brazil
J Biol Chem 279:33298-305. 2004
..Our results give support to conclude that the iron oxidation state is an important factor modulating the oligomeric dissociation...
- Hemoglobin mediated contraction of the isolated rat thoracic aorta: why is precontraction necessary?
H W Kim
Brown University and The Miriam Hospital, Providence, RI 02906, USA
Artif Cells Blood Substit Immobil Biotechnol 33:27-36. 2005
..This may explain why the Hb mediated additional contraction occurs only after an agonist induced precontraction...
- Role of thiamine thiol form in nitric oxide metabolism
A I Stepuro
Institute of Biochemistry, National Academy of Sciences of Belarus, Bulvar Keninskogo Komsomola 50, 230017 Grodno, Belarus
Biochemistry (Mosc) 70:339-49. 2005
..the release of nitric oxide was recorded by the production of nitrite or the oxidation of oxyhemoglobin to methemoglobin. The concentration of the thiol form in the body under physiological pH values (7.2-7.4) did not exceed 1.5-2...
- Nitric oxide scavenging by red blood cells as a function of hematocrit and oxygenation
Department of Physics, School of Medicine, Wake Forest University, Winston Salem, North Carolina 27109, USA
J Biol Chem 280:39024-32. 2005
..These observations may have important physiological implications within the microvasculature and for pathophysiological disruption of nitric oxide homeostasis in diseases...
- UVA-ketoprofen-induced hemoglobin radicals detected by immuno-spin trapping
Yu Ying He
Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA
Photochem Photobiol 77:585-91. 2003
..The methemoglobin (metHb) radicals react covalently with 5,5-dimethyl-1-pyrroline N-oxide (DMPO) to form nitroxyl radical ..
- Enalapril increases mitochondrial nitric oxide synthase activity in heart and liver
Laboratory of Free Radical Biology, School of Pharmacy and Biochemistry, University of Buenos Aires, Buenos Aires, Argentina
Antioxid Redox Signal 5:691-7. 2003
..as determined by dual wavelength spectrophotometry (577-591 nm) following hemoglobin oxidation to methemoglobin. The response to enalapril treatment, attributed to a gene-mediated up-regulation of mitochondrial nitric ..
- Effects of choline on hemorheological properties and NO metabolism of human erythrocytes
Institute of Biochemistry, Faculty of Medicine, University of Lisbon, Avenida Prof Egas Moniz, 1649 028 Lisbon, Portugal
Clin Hemorheol Microcirc 29:41-51. 2003
..To determine the effects of choline on red blood cell membrane properties and NO metabolism...
- The case of the missing NO-hemoglobin: spectral changes suggestive of heme redox reactions reflect changes in NO-heme geometry
Nicholas School of the Environment and Earth Sciences, Duke University, Beaufort, NC 28516, USA
Proc Natl Acad Sci U S A 100:12087-92. 2003
- A new sensitive assay reveals that hemoglobin is oxidatively modified in vivo
Niels B J Vollaard
Department of Biological Sciences, University of Essex, Wivenhoe Park, Colchester, CO4 3SQ, UK
Free Radic Biol Med 39:1216-28. 2005
..The exercise-induced increase is short-lived, suggesting the existence of an active mechanism for repairing or removing the damaged heme proteins...
- Noninvasive in vivo monitoring of methemoglobin formation and reduction with broadband diffuse optical spectroscopy
Laser Microbeam and Medical Program, Beckman Laser Institute, 1002 Health Sciences Rd East, University of California, Irvine, CA 92612 1475, USA
J Appl Physiol 100:615-22. 2006
We present noninvasive, quantitative in vivo measurements of methemoglobin formation and reduction in a rabbit model using broadband diffuse optical spectroscopy (DOS)...
- Characterization of hydrogen peroxide removal reaction by hemoglobin in the presence of reduced pyridine nucleotides
Department of Biochemistry, Okayama University Graduate School of Medicine and Dentistry, 2 5 1 Shikata cho, 700 8558, Okayama, Japan
Biochim Biophys Acta 1637:46-54. 2003
..We deduced that the removal reaction by hemoglobin included formations of methemoglobin and the ferryl radical and reduction of the latter with pyridine nucleotides...
- Electron spin resonance investigation of semiquinone radicals formed from the reaction of ubiquinone 0 with human oxyhemoglobin
Laboratories of Pharmacology and Chemistry, NIEHS, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA
J Biol Chem 277:6104-10. 2002
..Subsequently UQ(0) bound to the beta-93 cysteinyl residue of HbO(2) to form a covalent-binding adduct responsible for the broad spectrum...
- Acetylphenylhydrazine induced haemoglobin oxidation in erythrocytes studied by MÃ¶ssbauer spectroscopy
Instituto di Scienze Fisiche, INFM-Udr Parma, University of Parma, Italy
Biochim Biophys Acta 1568:99-104. 2001
- Apparent specific volume of human hemoglobin: effect of ligand state and contribution of heme
Section of Neurobiology, School of Biological Sciences, Institute of Molecular and Cell Biology, and Department of Chemistry and Biochemistry, University of Texas, Austin, TX 78712, USA
Anal Biochem 363:196-203. 2007
..The contribution can be estimated from the difference between the measured volume of each molecular species and that calculated from the amino acid composition...
- Reaction of melatonin with hemoglobin-derived oxoferryl radicals and inhibition of the hydroperoxide-induced hemoglobin denaturation in red blood cells
Department of Pharmacological Sciences, University of Palermo, Italy
J Pineal Res 31:114-9. 2001
..A decrease of heme loss and accumulation of soluble methemoglobin (met-Hb) are explained in terms of the interaction of the indoleamine with perferryl Hb (Hb[Fe(IV)=O]), a ..
- Inhaled nitric oxide improves survival rates during hypoxia in a sickle cell (SAD) mouse model
Department of Anesthesia Critical Care, Massachusetts General Hospital, Harvard Medical School, Boston, 02114, USA
Anesthesiology 94:1113-8. 2001
..Changes in HbSAD oxygen affinity were not detected with NO breathing, and methemoglobin levels were low in all surviving mice...
- Modulation of erythrocyte hemorheological properties by band 3 phosphorylation and dephosphorylation
Instituto de Biopatologia Química and Unidade de Biopatologia Vascular, Instituto de Medicina Molecular, Faculdade de Medicina de Lisboa, Lisboa, Portugal
Clin Hemorheol Microcirc 36:183-94. 2007
..In conclusion, the results suggest that erythrocyte aggregation index is dependent of the phosphorylated/dephosphorylated state of band 3...
- Transition of hemoglobin between two tertiary conformations: determination of equilibrium and thermodynamic parameters from the reaction of 5,5'-dithiobis(2-nitrobenzoate) with the CysF9beta sulfhydryl group
Kehinde Onwochei Okonjo
Department of Chemistry, University of Ibadan, Ibadan, Nigeria
Biophys Chem 128:56-62. 2007
..22+/-0.06. The standard enthalpy and entropy changes for the isomerization are DeltaH(o)(rt)=24.2 kJ mol(-1) and DeltaS(o)(rt)=68.8 JK(-1)mol(-1), respectively...
- [Effect of nebivolol on the blood oxygen transport]
V V Zinchuk
Eksp Klin Farmakol 70:44-7. 2007
..01), thereby indicating the dose dependence of the drug effect. Greater drug concentration resulted in higher methemoglobin level and nitrate/nitrite content...
- In vitro and in vivo kinetic handling of nitrite in blood: effects of varying hemoglobin oxygen saturation
Arlin B Blood
Center for Perinatal Research and Division of Neonatology, Loma Linda University School of Medicine, CA 92354, USA
Am J Physiol Heart Circ Physiol 293:H1508-17. 2007
..10 mg/kg iv) was given to normoxic or hypoxic newborn lambs, and the time course of plasma nitrite and methemoglobin (MetHb) concentrations was measured...
- The heme pocket geometry of Lucina pectinata hemoglobin II restricts nitric oxide and peroxide entry: model of ligand control for the design of a stable oxygen carrier
Walleska De Jesus-Bonilla
Department of Chemistry, University of Puerto Rico, Mayaguez Campus, P O Box 9019, Mayagüez 00681 9019, Puerto Rico
Biochemistry 46:10451-60. 2007
..Engineering of second-generation Hb-based oxygen therapeutics that are resistant to NO/H2O2-driven oxidation may ultimately require further optimization of the heme pocket architecture to limit heme exposure to solvent...
- Assay and characterization of the NO dioxygenase activity of flavohemoglobins
Paul R Gardner
Department of Chemistry, University of Dayton, Dayton, Ohio, USA
Methods Enzymol 436:217-37. 2008
..14.12.17) activity and function of flavohemoglobins are described. The methods may also be applied to the discovery and design of inhibitors for use as antibiotics or as modulators of *NO signaling...
- Separation of hemoglobin variants in single human erythrocytes by capillary electrophoresis with laser-induced native fluorescence detection
S J Lillard
Department of Chemistry, Iowa State University, Ames 50011, USA
J Chromatogr A 718:397-404. 1995
..Normal adult cells contained 9.6% and 4.8% glycated beta- and alpha-chains, respectively. Cells with elevated Hb A1 gave 30% and 12%, respectively. The amounts of glycated Hb and total Hb in a given cell were found to be uncorrelated...
- Iron nitrosyl hemoglobin formation from the reactions of hemoglobin and hydroxyurea
Department of Chemistry, Wake Forest University, Winston Salem, North Carolina 27109, USA
Biochemistry 41:2466-74. 2002
..Electron paramagnetic resonance spectroscopy shows that hydroxyurea reacts with oxy-, deoxy-, and methemoglobin to produce 2-6% of iron nitrosyl hemoglobin. No S-nitrosohemoglobin forms during these reactions...
- Inhibition of human hemoglobin autoxidation by sodium n-dodecyl sulphate
Dayer Mohammad Reza
Institute of Biochemistry and Biophysics, University of Tehran, Iran
J Biochem Mol Biol 35:364-70. 2002
..The induction of the deoxy form in the presence of SDS was confirmed by densitometry techniques. The compact structure of deoxyhemoglobin blocks the formation of met-conformation in low SDS concentrations...
- In vivo spectroscopy: a novel approach for simultaneously estimating nitric oxide and hemodynamic parameters in the rat brain
José L González-Mora
Departamento de Fisiologia, Facultad de Medicina, Universidad de La Laguna, Tenerife 38071, Canary Islands, Spain
J Neurosci Methods 119:151-61. 2002
..Under physiological conditions, NO reacts with oxyhemoglobin (OxyHb) to form methemoglobin (MetHb) at a very high rate...
- [Precision and accuracy of "a pocket" pulse oximeter in Mexico City]
Clínica de Suefio, Instituto Nacional de Enfermedades Respiratorias, Tlalpan, DF Mexico
Rev Invest Clin 58:28-33. 2006
..The objective was to evaluate the precision and accuracy of a "pocket" pulse oximeter at an altitude of 2,240 m above sea level...
- Interaction of sodium dodecyl sulfate with human native and cross-linked hemoglobins: a transient kinetic study
Apurba Kumar Sau
Department of Chemical Sciences, Tata Institute of Fundamental Research, Homi Bhabha Road, Colaba, 400 005, Mumbai, India
Biophys Chem 98:267-73. 2002
..83 mM) with human native and cross-linked oxyhemoglobin (oxyHb) and methemoglobin (metHb) has been investigated by optical spectroscopy and stopped-flow transient kinetic measurements...
- Site-specific cross-linking of human and bovine hemoglobins differentially alters oxygen binding and redox side reactions producing rhombic heme and heme degradation
Molecular Dynamics Section, National Institute on Aging NIH, 5600 Nathan Shock Drive, Baltimore, MD 21224 6823, USA
Biochemistry 41:7407-15. 2002
..These findings establish the importance of these secondary oxidative reactions over autoxidation in evaluating the toxicity of HBOCs...
- Potential interference by hydroxocobalamin on cooximetry hemoglobin measurements during cyanide and smoke inhalation treatments
Laser Microbeam and Medical Program, Beckman Laser Institute, University of California, Irvine, CA, USA
Ann Emerg Med 49:802-5. 2007
..peak at 532 nm), we investigate potential interference effects of OHCob on total hemoglobin concentration (tHb), carboxyhemoglobin (COHb), methemoglobin (MetHb), and oxyhemoglobin (Hb-O2) cooximetry measurement values in blood.
- Nitric oxide reaction with red blood cells and hemoglobin under heterogeneous conditions
Tae H Han
Department of Chemical Engineering, University of California, Los Angeles, CA 90095 1592, USA
Proc Natl Acad Sci U S A 99:7763-8. 2002
..These results were supported further by mathematically modeling NO-Hb reactions and diffusion...
- Ascorbate removes key precursors to oxidative damage by cell-free haemoglobin in vitro and in vivo
Department of Biological Sciences, University of Essex, Wivenhoe Park, Colchester CO4 3SQ, UK
Biochem J 399:513-24. 2006
..g. haemolytic anaemias, subarachnoid haemorrhage, rhabdomyolysis...
- Role of redox potential of hemoglobin-based oxygen carriers on methemoglobin reduction by plasma components
Scott C Dorman
Department of Chemistry, University of South Alabama, Mobile, USA
Artif Cells Blood Substit Immobil Biotechnol 30:39-51. 2002
..This is necessary for the reversible binding/release of molecular oxygen and minimization of methemoglobin (Fe+3) formation. Acellular hemoglobins are especially susceptible to oxidation and denaturation...
- Exposure to malondialdehyde induces an early redox unbalance preceding membrane toxicity in human erythrocytes
Department of Pharmaceutical Toxicological and Biological Chemistry, University of Palermo, Italy
Free Radic Res 36:89-97. 2002
..Accumulation of methemoglobin, and formation of small amounts of hemichrome were later evident...
- Oxygen-related processes in red blood cells exposed to tert-butyl hydroperoxide
I K Dremza
Institute of Biochemistry, National Academy of Sciences of Belarus, Grodno, Belarus
Redox Rep 11:185-92. 2006
..Neither GSH nor oxyhaemoglobin oxidation depended on oxygen pressure...
- The effect of simvastatin on erythrocyte membrane fluidity during oxidative stress induced by cardiopulmonary bypass: a randomized controlled study
Department of Biochemical Sciences, University La Sapienza, Rome, Italy
Clin Ther 29:1706-17. 2007
..Abnormal erythrocyte deformability can cause severe complications during cardiopulmonary bypass (CPB) surgery, including both hemolysis and perfusion abnormalities...
- Antidotal treatment of cyanide poisoning
Réanimation Médicale et Toxicologique, Universite Paris VII, INSERM U26, Hospital Lariboisiere, Paris, France
J Chin Med Assoc 66:193-203. 2003
..Oxygen counteracts efficiently cyanide action at the mitochondrial level. Sodium thiosulfate, methemoglobin forming agents and cobalt compounds act efficiently by complexing or transforming cyanide into non-toxic ..
- Mechanism of the contractile effect of diaspirin-cross-linked hemoglobin in rat isolated aorta strip denuded of endothelium as revealed using an oil-immersion procedure
Centre de Recherche, , , Canada
Can J Physiol Pharmacol 74:1171-9. 1996
..to drugs in the Krebs medium followed by replacement of the Krebs medium by mineral oil) was mimicked by methemoglobin and metmyoglobin, but not by cytochrome c, albumin, hemin, hematin, Fe2+, and a variety of hemorphins...
- Two cases of methemoglobinemia following zopiclone ingestion
Hin Tat Fung
Tuen Mun Hospital, Hong Kong, China
Clin Toxicol (Phila) 46:167-70. 2008
..Intoxication with zopiclone is expected to produce drowsiness, confusion and coma but not methemoglobinemia. We report two cases of zopiclone overdose with methemoglobinemia...
- The availability of toxicological analyses for poisoned patients in Ireland
National Poisons Information Centre, Beaumont Hospital, Dublin, Ireland
Clin Toxicol (Phila) 48:373-9. 2010
..Some professional associations in different countries quote these guidelines but there are no data to support adherence to these recommendations in the medical literature...
- Ascaris suum NADH-methemo(myo)globin reductase systems recovering differential functions of hemoglobin and myoglobin, adapting to environmental hypoxia
Department of Molecular and Cellular Parasitology, Juntendo University School of Medicine, 2 1 1 Hongo, Bunkyo ku, Tokyo 113 8421, Japan
Parasitol Int 58:278-84. 2009
..and dually localized in extracellular perienteric fluid and hypodermis, is involved in both perienteric NADH-methemoglobin and cytosolic NADH-metmyoglobin reduction, where cytochrome b5 functions as an electron carrier between NADH-..