streptavidin

Summary

Summary: A 60-kDa extracellular protein of Streptomyces avidinii with four high-affinity biotin binding sites. Unlike AVIDIN, streptavidin has a near neutral isoelectric point and is free of carbohydrate side chains.

Top Publications

  1. pmc Imaging proteins in live mammalian cells with biotin ligase and monovalent streptavidin
    Mark Howarth
    Department of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA
    Nat Protoc 3:534-45. 2008
  2. pmc A monovalent streptavidin with a single femtomolar biotin binding site
    Mark Howarth
    Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Nat Methods 3:267-73. 2006
  3. pmc A streptavidin variant with slower biotin dissociation and increased mechanostability
    Claire E Chivers
    Department of Biochemistry, Oxford University, UK
    Nat Methods 7:391-3. 2010
  4. pmc Streptavidin aptamers: affinity tags for the study of RNAs and ribonucleoproteins
    C Srisawat
    Department of Biological Chemistry, The University of Michigan, Ann Arbor 48109-0606, USA
    RNA 7:632-41. 2001
  5. ncbi The biotin-streptavidin interaction can be reversibly broken using water at elevated temperatures
    Anders Holmberg
    Department of Biotechnology, Royal Institute of Technology KTH, Stockholm, Sweden
    Electrophoresis 26:501-10. 2005
  6. pmc Biotin-4-fluorescein based fluorescence quenching assay for determination of biotin binding capacity of streptavidin conjugated quantum dots
    Rowena Mittal
    Department of Biomedical Engineering, Carnegie Mellon University, Pittsburgh, Pennsylvania, USA
    Bioconjug Chem 22:362-8. 2011
  7. ncbi Isolation of transcription factor complexes by in vivo biotinylation tagging and direct binding to streptavidin beads
    Patrick Rodriguez
    Department of Cell Biology, Erasmus Medical Center, Rotterdam, The Netherlands
    Methods Mol Biol 338:305-23. 2006
  8. ncbi Self-assembly of proteins into designed networks
    Philippe Ringler
    Institut fur Organische Chemie und Biochemie, Albert Ludwigs Universitat Freiburg, Albertstrasse 21, D 79104 Freiburg im Breisgau, Germany
    Science 302:106-9. 2003
  9. ncbi Templated protein assembly on micro-contact-printed surface patterns. Use of the SNAP-tag protein functionality
    Lars Iversen
    Bio Nanotechnology Laboratory, Department of Neuroscience and Pharmacology and Nano Science Center, University of Copenhagen, Universitetsparken 5, 2100 Copenhagen, Denmark
    Langmuir 24:6375-81. 2008
  10. ncbi Novel structural labeling method using cryo-electron tomography and biotin-streptavidin system
    Toshiyuki Oda
    Department of Cell Biology and Anatomy, Graduate School of Medicine, The University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    J Struct Biol 183:305-11. 2013

Detail Information

Publications365 found, 100 shown here

  1. pmc Imaging proteins in live mammalian cells with biotin ligase and monovalent streptavidin
    Mark Howarth
    Department of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA
    Nat Protoc 3:534-45. 2008
    ..The biotin group then allows sensitive and stable binding by streptavidin conjugates...
  2. pmc A monovalent streptavidin with a single femtomolar biotin binding site
    Mark Howarth
    Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Nat Methods 3:267-73. 2006
    b>Streptavidin and avidin are used ubiquitously because of the remarkable affinity of their biotin binding, but they are tetramers, which disrupts many of their applications...
  3. pmc A streptavidin variant with slower biotin dissociation and increased mechanostability
    Claire E Chivers
    Department of Biochemistry, Oxford University, UK
    Nat Methods 7:391-3. 2010
    b>Streptavidin binds biotin conjugates with exceptional stability but dissociation does occur, limiting its use in imaging, DNA amplification and nanotechnology...
  4. pmc Streptavidin aptamers: affinity tags for the study of RNAs and ribonucleoproteins
    C Srisawat
    Department of Biological Chemistry, The University of Michigan, Ann Arbor 48109-0606, USA
    RNA 7:632-41. 2001
    ..To develop a new affinity tag, streptavidin-binding RNA ligands, termed "aptamers," were identified from a random RNA library using in vitro ..
  5. ncbi The biotin-streptavidin interaction can be reversibly broken using water at elevated temperatures
    Anders Holmberg
    Department of Biotechnology, Royal Institute of Technology KTH, Stockholm, Sweden
    Electrophoresis 26:501-10. 2005
    The biotin-streptavidin system is the strongest noncovalent biological interaction known, having a dissociation constant, K(d), in the order of 4x10(-14) M...
  6. pmc Biotin-4-fluorescein based fluorescence quenching assay for determination of biotin binding capacity of streptavidin conjugated quantum dots
    Rowena Mittal
    Department of Biomedical Engineering, Carnegie Mellon University, Pittsburgh, Pennsylvania, USA
    Bioconjug Chem 22:362-8. 2011
    ..Commercially available streptavidin conjugates (SAv QDs) are the most commonly used tool for preparing QD-biomolecule conjugates...
  7. ncbi Isolation of transcription factor complexes by in vivo biotinylation tagging and direct binding to streptavidin beads
    Patrick Rodriguez
    Department of Cell Biology, Erasmus Medical Center, Rotterdam, The Netherlands
    Methods Mol Biol 338:305-23. 2006
    ..Owing to the very high affinity of biotin for avidin and streptavidin, biotinylation tagging offers an attractive approach for the efficient purification of protein complexes...
  8. ncbi Self-assembly of proteins into designed networks
    Philippe Ringler
    Institut fur Organische Chemie und Biochemie, Albert Ludwigs Universitat Freiburg, Albertstrasse 21, D 79104 Freiburg im Breisgau, Germany
    Science 302:106-9. 2003
    ..was used to produce a quadratic network consisting of the enzyme as a rigid four-way connector and stiff streptavidin rods as spacers...
  9. ncbi Templated protein assembly on micro-contact-printed surface patterns. Use of the SNAP-tag protein functionality
    Lars Iversen
    Bio Nanotechnology Laboratory, Department of Neuroscience and Pharmacology and Nano Science Center, University of Copenhagen, Universitetsparken 5, 2100 Copenhagen, Denmark
    Langmuir 24:6375-81. 2008
    ..When patterns of streptavidin modified with BG-biotin (streptavidin-BG) are stamped onto a surface, the SFH can subsequently assemble on the ..
  10. ncbi Novel structural labeling method using cryo-electron tomography and biotin-streptavidin system
    Toshiyuki Oda
    Department of Cell Biology and Anatomy, Graduate School of Medicine, The University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    J Struct Biol 183:305-11. 2013
    ..Here, we report a novel method for identifying the 3D locations of proteins using biotin-streptavidin labeling and cryo-electron tomography...
  11. pmc DOLORS: versatile strategy for internal labeling and domain localization in electron microscopy
    Pick Wei Lau
    National Resource for Automated Molecular Microscopy, The Scripps Research Institute, La Jolla, CA 92037, USA
    Structure 20:1995-2002. 2012
    ..DOLORS uses monovalent streptavidin added posttranslationally to tagged sites in the target protein...
  12. ncbi Multimodality nuclear and fluorescence tumor imaging in mice using a streptavidin nanoparticle
    Minmin Liang
    Department of Radiology, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
    Bioconjug Chem 21:1385-8. 2010
    ..We describe the use of streptavidin nanoparticle-based complexes as multimodality imaging agents to achieve tumor detection in a mouse model by ..
  13. ncbi One-step purification of recombinant proteins using a nanomolar-affinity streptavidin-binding peptide, the SBP-Tag
    A D Keefe
    Department of Molecular Biology, Howard Hughes Medical Institute, Boston, Massachusetts 02114, USA
    Protein Expr Purif 23:440-6. 2001
    We describe the use of the SBP-tag, a new streptavidin-binding peptide, for both the one-step purification and the detection of recombinant proteins...
  14. ncbi Multiplex transport and detection of cytokines using kinesin-driven molecular shuttles
    Lynnette Rios
    Biomolecular Interfaces and Systems Department, Sandia National Laboratories, PO Box 5800, MS 1303, Albuqeurque, NM 87185 1303, USA
    Lab Chip 9:1005-10. 2009
    ..antibodies against interleukin-2 (IL-2) and tumor necrosis factor-alpha (TNF-alpha) on biotinylated MTs using a streptavidin bridge...
  15. pmc Interaction of streptavidin-based peptide-MHC oligomers (tetramers) with cell-surface TCRs
    Jennifer D Stone
    Department of Biochemistry, University of Illinois, Urbana Champaign, Urbana, IL 61801, USA
    J Immunol 187:6281-90. 2011
    ..In this study, we analyzed the equilibrium binding of streptavidin-based pMHC oligomers (tetramers) and their dissociation kinetics from CD8(pos) T cells from 2C-TCR transgenic ..
  16. ncbi Engineering soluble monomeric streptavidin with reversible biotin binding capability
    Sau Ching Wu
    Department of Biological Sciences, University of Calgary, Alberta, Canada
    J Biol Chem 280:23225-31. 2005
    Monomeric streptavidin with reversible biotin binding capability has many potential applications...
  17. ncbi Quantitative measurement of IgE antibodies to purified allergens using streptavidin linked to a high-capacity solid phase
    Elizabeth A Erwin
    University of Virginia Astma and Allergic Diseases Center, Charlottesville, VA 22908 1355, USA
    J Allergy Clin Immunol 115:1029-35. 2005
    ..Commercially available assays for IgE antibody provide results in international units per milliliter for many allergen extracts, but this is not easily achieved with purified or novel allergens...
  18. pmc A two-photon excitation fluorescence cross-correlation assay for a model ligand-receptor binding system using quantum dots
    J L Swift
    Department of Chemistry, University of Calgary, Calgary AB, T2N 1N4, Canada
    Biophys J 90:1396-410. 2006
    ..Nanocrystalline semiconductor quantum dots functionalized with biotin (lambda(em) = 605 nm) and streptavidin (lambda(em) = 525 nm) were used for which an average K(d) value of 0.30 +/- 0.04 x 10(-9) M was obtained (cf...
  19. pmc Engineered single-chain dimeric streptavidins with an unexpected strong preference for biotin-4-fluorescein
    Filiz M Aslan
    Center for Advanced Biotechnology, Department of Chemistry, and Biomolecular Engineering Research Center, Boston University, Boston, MA 02215, USA
    Proc Natl Acad Sci U S A 102:8507-12. 2005
    b>Streptavidin, a homotetrameric protein with extremely tight biotin binding (K(d) < or = 10(-14) M), has been widely used as an affinity reagent...
  20. pmc The use of mRNA display to select high-affinity protein-binding peptides
    D S Wilson
    Howard Hughes Medical Institute and Department of Molecular Biology, Massachusetts General Hospital, Boston, MA 02114, USA
    Proc Natl Acad Sci U S A 98:3750-5. 2001
    ..Starting with a library of approximately 10(13) random peptides, 20 different aptamers to streptavidin were obtained, with dissociation constants as low as 5 nM...
  21. ncbi A nanoscale optical biosensor: sensitivity and selectivity of an approach based on the localized surface plasmon resonance spectroscopy of triangular silver nanoparticles
    Amanda J Haes
    Department of Chemistry, Northwestern University, Evanston, Illinois 60208 3113, USA
    J Am Chem Soc 124:10596-604. 2002
    ..and operational principles of these LSPR nanobiosensors will be illustrated using the well-studied biotin-streptavidin system. Exposure of biotin-functionalized Ag nanotriangles to 100 nM streptavidin (SA) caused a 27...
  22. ncbi Genetically engineered avidins and streptavidins
    O H Laitinen
    A I Virtanen Institute, Department of Molecular Medicine, University of Kuopio, P O Box 1627, 70211, Kuopio, Finland
    Cell Mol Life Sci 63:2992-3017. 2006
    Chicken avidin and bacterial streptavidin, (strept)avidin, are proteins widely utilized in a number of applications in life science, ranging from purification and labeling techniques to diagnostics, and from targeted drug delivery to ..
  23. pmc Three-dimensional structures of avidin and the avidin-biotin complex
    O Livnah
    Department of Structural Biology, Weizmann Institute of Science, Rehovot, Israel
    Proc Natl Acad Sci U S A 90:5076-80. 1993
    ..antiparallel beta-barrel, remarkably similar to that of the genetically distinct bacterial analog streptavidin. As in streptavidin, binding of biotin involves a highly stabilized network of polar and hydrophobic ..
  24. ncbi FluMag-SELEX as an advantageous method for DNA aptamer selection
    R Stoltenburg
    Department of Environmental Biotechnology, Centre for Environmental Research Leipzig Halle GmbH UFZ, Permoserstr 15, 04318 Leipzig, Germany
    Anal Bioanal Chem 383:83-91. 2005
    ..These aptamers will be applied as new biosensor receptors. In this work selection of streptavidin-specific aptamers by FluMag-SELEX is described...
  25. ncbi Brave new (strept)avidins in biotechnology
    Olli H Laitinen
    A I Virtanen Institute, Department of Molecular Medicine, University of Kuopio, P O Box 1627, FI 70211 Kuopio, Finland
    Trends Biotechnol 25:269-77. 2007
    Avidin and streptavidin are widely used in (strept)avidin-biotin technology, which is based on their tight biotin-binding capability...
  26. ncbi Conservative secondary structure motif of streptavidin-binding aptamers generated by different laboratories
    Tao Bing
    Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China
    Bioorg Med Chem 18:1798-805. 2010
    ..Here we report the selection of a group of aptamer candidates (85 mer) against streptavidin. Through comparing the predicted secondary structures of all the candidates, a conservative bulge-hairpin ..
  27. ncbi Hepatitis C virus NS3 and simian virus 40 T antigen helicases displace streptavidin from 5'-biotinylated oligonucleotides but not from 3'-biotinylated oligonucleotides: evidence for directional bias in translocation on single-stranded DNA
    Patrick D Morris
    Department of Biochemistry and Molecular Biology, University of Arkansas for Medical Sciences, Little Rock, Arkansas 72205, USA
    Biochemistry 41:2372-8. 2002
    ..We developed an assay that monitors the ability of a helicase to displace streptavidin from biotinylated oligonucleotides [Morris, P. D., and Raney, K. D. (1999) Biochemistry 38, 5164-5171]...
  28. pmc Cooperative hydrogen bond interactions in the streptavidin-biotin system
    David E Hyre
    Box 351721, Department of Bioengineering, University of Washington, Seattle, WA 98195 1721, USA
    Protein Sci 15:459-67. 2006
    ..bonds was measured by calorimetric and X-ray crystallographic studies of the S45A/D128A double mutant of streptavidin. The double mutant exhibits a binding affinity approximately 2x10(7) times lower than that of wild-type ..
  29. ncbi Enhanced antigen-specific antibody and cytokine responses when targeting antigen to human FcGAMMA receptor type I using an anti-human FcGAMMA receptor type I-streptavidin fusion protein in an adjuvant-free system
    Elisaveta Adamova
    Center for Immunology and Microbial Disease, MC 151, Albany Medical College, Albany, NY 12208, USA
    Immunol Invest 34:417-29. 2005
    ..Furthermore, antibody isotype and cytokine responses following immunization with hFcgammaRI-targeted antigen, suggest enhancement of both Th1 and Th2 responses...
  30. ncbi One-step affinity purification of bacterially produced proteins by means of the "Strep tag" and immobilized recombinant core streptavidin
    T G Schmidt
    Abteilung Molekulare Membranbiologie, , Frankfurt am Main, Germany
    J Chromatogr A 676:337-45. 1994
    The "Strep tag" is a nine amino acid peptide with intrinsic streptavidin-binding activity...
  31. ncbi Tracking T cells with tetramers: new tales from new tools
    Paul Klenerman
    Nuffield Department of Medicine, University of Oxford, UK
    Nat Rev Immunol 2:263-72. 2002
    ..Although there are common patterns, over time, each host-pathogen relationship seems to develop unique characteristics, as reflected in the quality of the T-cell response...
  32. ncbi Replacement-free electrodeless quartz crystal microbalance biosensor using nonspecific-adsorption of streptavidin on quartz
    Hirotsugu Ogi
    Graduate School of Engineering Science, Osaka University, Machikaneyama 1 3, Toyonaka, Osaka 560 8531, Japan
    Anal Chem 81:4015-20. 2009
    ..With the use of significant nonspecific adsorption of streptavidin on naked quartz surfaces, target analyte is detected through biotin-tagged receptors on streptavidin...
  33. pmc Real-time biomolecular binding detection using a sensitive photonic crystal biosensor
    Yunbo Guo
    Center for Ultrafast Optical Science, Department of Electrical Engineering and Computer Science, University of Michigan, Ann Arbor, Michigan 48109, USA
    Anal Chem 82:5211-8. 2010
    ..We characterize the performance of this biosensor using a standard streptavidin-biotin binding system...
  34. ncbi On-chip microlasers for biomolecular detection via highly localized deposition of a multifunctional phospholipid ink
    Uwe Bog
    Institute of Microstructure Technology, Karlsruhe Institute of Technology, 76128 Karlsruhe, Germany
    Lab Chip 13:2701-7. 2013
    ..In a proof-of-concept we show how this highly localized molecule deposition suffices for low-threshold lasing in air and water, and demonstrate the capability of the ink-lasers as biosensors in a biotin-streptavidin binding experiment.
  35. pmc Intersubunit contacts made by tryptophan 120 with biotin are essential for both strong biotin binding and biotin-induced tighter subunit association of streptavidin
    T Sano
    Center for Advanced Biotechnology, Boston University, MA 02215, USA
    Proc Natl Acad Sci U S A 92:3180-4. 1995
    In natural streptavidin, tryptophan 120 of each subunit makes contacts with the biotin bound by an adjacent subunit through the dimer-dimer interface...
  36. pmc How the biotin-streptavidin interaction was made even stronger: investigation via crystallography and a chimaeric tetramer
    Claire E Chivers
    Department of Biochemistry, Oxford University, South Parks Road, Oxford OX1 3QU, UK
    Biochem J 435:55-63. 2011
    The interaction between SA (streptavidin) and biotin is one of the strongest non-covalent interactions in Nature. SA is a widely used tool and a paradigm for protein-ligand interactions...
  37. ncbi Smart polymer-streptavidin conjugates
    Patrick S Stayton
    Department of Bioengineering, University of Washington, Seattle, USA
    Methods Mol Biol 283:37-43. 2004
    The conjugation of stimuli-responsive, or "smart," polymers to streptavidin is described...
  38. pmc Biotinylated Rh(III) complexes in engineered streptavidin for accelerated asymmetric C-H activation
    Todd K Hyster
    Department of Chemistry, Colorado State University, Fort Collins, CO 80523, USA
    Science 338:500-3. 2012
    ..of a bifunctional artificial metalloenzyme in which a glutamic acid or aspartic acid residue engineered into streptavidin acts in concert with a docked biotinylated rhodium(III) complex to enable catalytic asymmetric carbon-hydrogen (..
  39. pmc Streptavidin-Binding Peptide (SBP)-tagged SMC2 allows single-step affinity fluorescence, blotting or purification of the condensin complex
    Ji Hun Kim
    Murdoch Childrens Research Institute, Royal Children s Hospital, Melbourne, Victoria, Australia
    BMC Biochem 11:50. 2010
    ..Often protein purification, cellular localisation and Western blot analyses can be multi-step processes, where protein is lost, activity is destroyed or effective antibodies have not yet been generated...
  40. ncbi Sodium dodecyl sulfate-polyacrylamide gel electrophoretic method for assessing the quaternary state and comparative thermostability of avidin and streptavidin
    E A Bayer
    Department of Membrane Research and Biophysics, Weizmann Institute of Science, Rehovot, Israel
    Electrophoresis 17:1319-24. 1996
    ..Acetylated avidin was found to behave in a manner similar to that of streptavidin; under nondenaturing conditions (i.e...
  41. pmc Streptavidin facilitates internalization and pulmonary targeting of an anti-endothelial cell antibody (platelet-endothelial cell adhesion molecule 1): a strategy for vascular immunotargeting of drugs
    V R Muzykantov
    Institute for Environmental Medicine, University of Pennsylvania, Philadelphia, PA, 19104 60608, USA
    Proc Natl Acad Sci U S A 96:2379-84. 1999
    ..However, conjugation of biotinylated anti-PECAM (b-anti-PECAM) with streptavidin (SA) markedly stimulated uptake and internalization of anti-PECAM by endothelial cells and by cells expressing ..
  42. ncbi C2-streptavidin mediates the delivery of biotin-conjugated tumor suppressor protein p53 into tumor cells
    Jörg Fahrer
    Institute of Pharmacology and Toxicology, University of Ulm Medical Center, Albert Einstein Allee 11, D 89081 Ulm, Germany
    Bioconjug Chem 24:595-603. 2013
    We have previously generated a recombinant C2-streptavidin fusion protein for the delivery of biotin-labeled molecules of low molecular weight into the cytosol of mammalian cells...
  43. ncbi A biosensor based on photonic crystal surface waves with an independent registration of the liquid refractive index
    Valery N Konopsky
    Institute of Spectroscopy, Russian Academy of Sciences, Troitsk, Moscow Region, Russia
    Biosens Bioelectron 25:1212-6. 2010
    ..This technique was tested using binding of biotin molecules to a streptavidin monolayer at low and high biotin concentrations...
  44. ncbi High resolution structure of streptavidin in complex with a novel high affinity peptide tag mimicking the biotin binding motif
    M Perbandt
    Department of Biochemistry and Molecular Biology, University of Hamburg, 20146 Hamburg, Germany
    Proteins 67:1147-53. 2007
    A novel peptide was designed which possesses nanomolar affinity of less than 20 nM for streptavidin. Therefore it was termed Nano-tag and has been used as an affinity tag for recombinant proteins...
  45. pmc Thermal and sodium dodecylsulfate induced transitions of streptavidin
    Mark J Waner
    Department of Chemistry, John Carroll University, University Heights, Ohio 44118, USA
    Biophys J 87:2701-13. 2004
    The strong specific binding of streptavidin (SA) to biotin is utilized in numerous biotechnological applications. The SA tetramer is also known to exhibit significant stability, even in the presence of sodium dodecylsulfate (SDS)...
  46. ncbi PET imaging of apoptosis with (64)Cu-labeled streptavidin following pretargeting of phosphatidylserine with biotinylated annexin-V
    Nicole Cauchon
    Sherbrooke Molecular Imaging Centre and Department of Nuclear Medicine and Radiobiology, Faculty of Medicine and Health Sciences, Universite de Sherbrooke, Sherbrooke, QC, Canada
    Eur J Nucl Med Mol Imaging 34:247-58. 2007
    ..In this study, tumor apoptosis after therapy was visualized by positron emission tomography (PET) using (64)Cu-labeled streptavidin (SAv), following pre-targeting of apoptotic cells with biotinylated anxV.
  47. pmc Effects of multiple-bond ruptures on kinetic parameters extracted from force spectroscopy measurements: revisiting biotin-streptavidin interactions
    Senli Guo
    Department of Chemistry, Duke University, Durham, North Carolina 27708, USA
    Biophys J 95:3964-76. 2008
    Force spectroscopy measurements of the rupture of the molecular bond between biotin and streptavidin often results in a wide distribution of rupture forces...
  48. pmc Engineering of a Bacillus subtilis strain with adjustable levels of intracellular biotin for secretory production of functional streptavidin
    Sau Ching Wu
    Department of Biological Sciences, Division of Molecular, Cellular and Microbial Biology, University of Calgary, 2500 University Drive NW, Calgary, Alberta T2N 1N4, Canada
    Appl Environ Microbiol 68:1102-8. 2002
    b>Streptavidin is a biotin-binding protein which has been widely used in many in vitro and in vivo applications...
  49. ncbi A novel immunotherapy for superficial bladder cancer by the immobilization of streptavidin-tagged bioactive IL-2 on the biotinylated mucosal surface of the bladder wall
    Xin Huang
    Institute of Biotherapy, School of Biotechnology, Southern Medical University, Guangzhou, Guangdong 510515, PR China
    Chin J Cancer 29:611-6. 2010
    ..This study established a novel immunotherapy through immobilization of streptavidin-tagged human IL-2 (SA-hIL-2) on the biotinylated mucosal surface of bladder wall.
  50. pmc Streptavidin and its biotin complex at atomic resolution
    Isolde Le Trong
    Department of Biological Structure, University of Washington, Seattle, USA
    Acta Crystallogr D Biol Crystallogr 67:813-21. 2011
    Atomic resolution crystallographic studies of streptavidin and its biotin complex have been carried out at 1.03 and 0.95 Å, respectively...
  51. pmc In vivo biotinylation of bacterial magnetic particles by a truncated form of Escherichia coli biotin ligase and biotin acceptor peptide
    Yoshiaki Maeda
    Department of Biotechnology, Tokyo University of Agriculture and Technology, 2 24 16 Naka cho, Koganei, Tokyo 184 8588, Japan
    Appl Environ Microbiol 76:5785-90. 2010
    ..The biotinylated BAP-displaying BacMPs were then exposed to streptavidin by simple mixing...
  52. ncbi Coupling of biotin-(poly(ethylene glycol))amine to poly(D,L-lactide-co-glycolide) nanoparticles for versatile surface modification
    Barbara Weiss
    Biopharmaceutics and Pharmaceutical Technology, Center for Bioinformatics, Saarland University, Saarbruecken, Germany
    Bioconjug Chem 18:1087-94. 2007
    ..Furthermore, after coupling of NAv-linked fluorescence dyes to BPEG-functionalized NP, differences in binding and uptake could be demonstrated using two epithelial cell lines (Caco-2, A549)...
  53. ncbi Antibody pretargeting advances cancer radioimmunodetection and radioimmunotherapy
    David M Goldenberg
    Garden State Cancer Center, Center for Molecular Medicine and Immunology, Belleville, NJ 07109, USA
    J Clin Oncol 24:823-34. 2006
    ..We describe initial promising clinical results using streptavidin-antibody constructs with biotin-radionuclide conjugates in the treatment of patients with malignant gliomas, ..
  54. ncbi In vitro selection of high-affinity DNA aptamers for streptavidin
    Chenglong Wang
    Department of Stomatology, General Hospital of PLA, Beijing 100853, China
    Acta Biochim Biophys Sin (Shanghai) 41:335-40. 2009
    ..As an example, the selection of streptavidin-specific aptamers was performed...
  55. ncbi Dissecting the streptavidin-biotin interaction by phage-displayed shotgun scanning
    Sara K Avrantinis
    Department of Chemistry 516 Rowland Hall, University of California Irvine, CA 92697 2025, USA
    Chembiochem 3:1229-34. 2002
    Shotgun scanning the streptavidin-biotin interaction identifies long-range hydrophobic interactions that contribute to one of the strongest naturally occurring noncovalent protein-ligand interactions...
  56. pmc The origins of femtomolar protein-ligand binding: hydrogen-bond cooperativity and desolvation energetics in the biotin-(strept)avidin binding site
    Jason DeChancie
    Department of Chemistry and Biochemistry, University of California, Los Angeles, California 90095 1569, USA
    J Am Chem Soc 129:5419-29. 2007
    The unusually strong reversible binding of biotin by avidin and streptavidin has been investigated by density functional and MP2 ab initio quantum mechanical methods...
  57. ncbi Accurate titration of avidin and streptavidin with biotin-fluorophore conjugates in complex, colored biofluids
    H J Gruber
    Institute of Biophysics, J Kepler University, Altenberger Str 69, A 4040 Linz, Austria
    Biochim Biophys Acta 1381:203-12. 1998
    A new fluorimetric assay is presented for the specific and reliable quantitation of >/=2 nM avidin and streptavidin. The assay is based on pronounced changes in the fluorescence properties of commercial fluorescein-biotin, or of a ..
  58. pmc Efficient biotinylation and single-step purification of tagged transcription factors in mammalian cells and transgenic mice
    Ernie de Boer
    Department of Cell Biology, Erasmus Medical Center, P O Box 1738, 3000 DR Rotterdam, The Netherlands
    Proc Natl Acad Sci U S A 100:7480-5. 2003
    ..is an attractive approach for protein complex purification due to the very high affinity of avidin/streptavidin for biotinylated templates...
  59. ncbi Analysis of protein-DNA binding by streptavidin-agarose pulldown
    Kenneth K Wu
    Division of Hematology, Institute of Molecular Medicine, University of Texas Health Science Center, Houston, TX, USA
    Methods Mol Biol 338:281-90. 2006
    ..We describe here a streptavidin-agarose pulldown assay that is capable of analyzing quantitatively binding of an array of proteins to DNA ..
  60. pmc Bacterial display using circularly permuted outer membrane protein OmpX yields high affinity peptide ligands
    Jeffrey J Rice
    Department of Chemical Engineering, University of California, Santa Brabara, 93106, USA
    Protein Sci 15:825-36. 2006
    ..Using streptavidin as a model target, bacterial display yielded the well-characterized HP(Q)/(M) motif obtained previously using ..
  61. ncbi T7 lytic phage-displayed peptide libraries exhibit less sequence bias than M13 filamentous phage-displayed peptide libraries
    Lauren R H Krumpe
    Basic Research Program, SAIC Frederick, Inc, Frederick, MD, USA
    Proteomics 6:4210-22. 2006
    ..To demonstrate their utility, several of the T7-displayed peptide libraries were screened for streptavidin- and neutravidin-binding phage. Novel binding motifs were identified for each protein.
  62. ncbi Easily reversible desthiobiotin binding to streptavidin, avidin, and other biotin-binding proteins: uses for protein labeling, detection, and isolation
    James D Hirsch
    Molecular Probes, Inc, Eugene, OR 97402, USA
    Anal Biochem 308:343-57. 2002
    The high-affinity binding of biotin to avidin, streptavidin, and related proteins has been exploited for decades...
  63. pmc The solution to the streptavidin-biotin paradox: the influence of history on the strength of single molecular bonds
    Frederic Pincet
    Laboratoire de Physique Statistique de l Ecole Normale Superieure, Paris, France
    Biophys J 89:4374-81. 2005
    ..the experiment even if the pulling conditions are similar: this paradox was first observed on the widely used streptavidin-biotin bond...
  64. pmc Aptamer-based endocytosis of a lysosomal enzyme
    Chi hong B Chen
    Department of Biological Chemistry, David Geffen School of Medicine at the University of California, Los Angeles, CA 90095 1737, USA
    Proc Natl Acad Sci U S A 105:15908-13. 2008
    ..After selection, an RNA aptamer and a DNA aptamer were modified with biotin and linked to dye-labeled streptavidin for detection by confocal microscopy...
  65. ncbi Bioactivation and cell targeting of semiconductor CdSe/ZnS nanocrystals with phytochelatin-related peptides
    Fabien Pinaud
    Department of Chemistry and Biochemistry, University of California Los Angeles, Los Angeles, California 90095, USA
    J Am Chem Soc 126:6115-23. 2004
    ..CdSe/ZnS nanocrystals coated with biotinylated peptides efficiently bind to streptavidin and are specifically targeted to GPI-anchored avidin-CD14 chimeric proteins expressed on the membranes of live ..
  66. ncbi Development and characterization of a series of soluble tetrameric and monomeric streptavidin muteins with differential biotin binding affinities
    M H Qureshi
    Department of Biological Sciences, Division of Cellular, Molecular and Microbial Biology, University of Calgary, Alberta T2N 1N4, Canada
    J Biol Chem 276:46422-8. 2001
    The strong biotin-streptavidin interaction limits the application of streptavidin as a reversible affinity matrix for purification of biotinylated biomolecules...
  67. ncbi Setting up roadblocks for kinesin-1: mechanism for the selective speed control of cargo carrying microtubules
    Till Korten
    Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, D 01307, Dresden, Germany
    Lab Chip 8:1441-7. 2008
    ..microtubules we found that individual kinesin-1 motors frequently stopped upon encounters with attached streptavidin molecules...
  68. ncbi A smart dust biosensor powered by kinesin motors
    Thorsten Fischer
    University of Florida, Department of Materials Science and Engineering, 160 Rhines Hall, Gainesville, Florida 32611 6400, USA
    Nat Nanotechnol 4:162-6. 2009
    ..Owing to their small size and autonomous function, we envision that large numbers of such smart dust biosensors could be inserted into organisms or distributed into the environment for remote sensing...
  69. ncbi Molecular self-assembly of "nanowires"and "nanospools" using active transport
    Henry Hess
    Department of Bioengineering, University of Washington, Seattle, Washington 98195, USA
    Nano Lett 5:629-33. 2005
    ..example, functionalized microtubules transported by surface-immobilized kinesin motors cross-link via biotin/streptavidin bonds and form extended linear and circular mesoscopic structures, which move in the presence of ATP...
  70. ncbi Generation and iterative affinity maturation of antibodies in vitro using hypermutating B-cell lines
    Sarah J Cumbers
    Medical Research Laboratory of Biology, Hills Road, Cambridge CB2 2QH, UK
    Nat Biotechnol 20:1129-34. 2002
    ..line expressing IgM of unknown specificity, we derived descendants that exhibit stepwise improved binding to streptavidin. Binding is initially conferred by mutations in complementarity-determining regions (CDRs), but maturation is ..
  71. ncbi Heterotetramers formed by an S-layer-streptavidin fusion protein and core-streptavidin as a nanoarrayed template for biochip development
    Carina Huber
    Center for NanoBiotechnology, University of Natural Resources and Applied Life Sciences Vienna, Gregor Mendel Strasse 33, 1180 Vienna, Austria
    Small 2:142-50. 2006
    Based on the S-layer protein SbpA of Bacillus sphaericus CCM 2177, an S-layer-streptavidin fusion protein was constructed...
  72. pmc Self-assembly of DNA-streptavidin nanostructures and their use as reagents in immuno-PCR
    C M Niemeyer
    Universitat Bremen, FB 2 UFT, Biotechnologie und Molekulare Genetik, Leobener Strasse, D 28359 Bremen, Germany
    Nucleic Acids Res 27:4553-61. 1999
    The self-assembly of bis-biotinylated double-stranded DNA and the tetravalent biotin-binding protein streptavidin (STV) have been studied by non-denaturing gel electrophoresis and atomic force microscopy (AFM)...
  73. pmc Evidence for rotation of V1-ATPase
    Hiromi Imamura
    ATP System Project, Exploratory Research for Advanced Technology, Japan Science and Technology Corporation, 5800 3 Nagatsuta, Midori ku, Yokohama 226 0026, Japan
    Proc Natl Acad Sci U S A 100:2312-5. 2003
    ..was immobilized onto a glass surface, and a bead was attached to the D or F subunit through the biotin-streptavidin linkage...
  74. ncbi The C2-streptavidin delivery system promotes the uptake of biotinylated molecules in macrophages and T-leukemia cells
    Jörg Fahrer
    Institute of Pharmacology and Toxicology, University of Ulm Medical Center, Albert Einstein Allee 11, Ulm, Germany
    Biol Chem 391:1315-25. 2010
    ..recently established a novel protein delivery system based on the binary C2 toxin of Clostridium botulinum and streptavidin, allowing the uptake of exogenous biotinylated molecules into mammalian cells...
  75. pmc An azido-biotin reagent for use in the isolation of protein adducts of lipid-derived electrophiles by streptavidin catch and photorelease
    Hye Young H Kim
    Department of Chemistry, Vanderbilt University, Nashville, Tennessee 37235, USA
    Mol Cell Proteomics 8:2080-9. 2009
    ..Peptides and proteins modified in this way are affinity purified on streptavidin beads...
  76. pmc Improved affinity of engineered streptavidin for the Strep-tag II peptide is due to a fixed open conformation of the lid-like loop at the binding site
    Ingo P Korndörfer
    Lehrstuhl fur Biologische Chemie, Technische Universitat Munchen, Freising Weihenstephan, Germany
    Protein Sci 11:883-93. 2002
    ..acid peptide that was developed as an affinity tool for the purification of corresponding fusion proteins on streptavidin columns...
  77. ncbi Biotin-streptavidin-labeled oligonucleotides as probes of helicase mechanisms
    P D Morris
    Department of Biochemistry and Molecular Biology, University of Arkansas for Medical Sciences, 4301 West Markham Street, Little Rock, Arkansas 72205, USA
    Methods 23:149-59. 2001
    ..To prepare such protein blocks, biotin-streptavidin has been used due to the ease with which the biotin can be incorporated into nucleic acids by chemical ..
  78. ncbi Streptavidin-biotin binding energetics
    P S Stayton
    Department of Bioengineering, University of Washington, Seattle 98195, USA
    Biomol Eng 16:39-44. 1999
    The high affinity energetics in the streptavidin-biotin system provide an excellent model system for studying how proteins balance enthalpic and entropic components to generate an impressive overall free energy for ligand binding...
  79. ncbi Protein biosensors based on biofunctionalized conical gold nanotubes
    Zuzanna Siwy
    Department of Chemistry and Center for Research at the Bio Nano Interface, University of Florida, Gainesville, Florida 32611 7200, USA
    J Am Chem Soc 127:5000-1. 2005
    ..different molecular-recognition agents, and correspondingly three different protein analytes, were investigated: (i) biotin/streptavidin, (ii) protein-G/immunoglobulin, and (iii) an antibody to the protein ricin with ricin as the analyte.
  80. ncbi Energy landscape roughness of the streptavidin-biotin interaction
    Felix Rico
    Department of Physiology and Biophysics, University of Miami Miller School of Medicine, 1600 N W 10th Avenue, Miami, FL 33136, USA
    J Mol Recognit 20:495-501. 2007
    ..The streptavidin-biotin complex has been extensively studied due to its remarkably low dissociation kinetics...
  81. ncbi Homogeneous noncompetitive assay of protein via Förster-resonance-energy-transfer with tryptophan residue(s) as intrinsic donor(s) and fluorescent ligand as acceptor
    Fei Liao
    Chongqing Key Laboratory of Biochemical and Molecular Pharmacology, Chongqing Medical University, Chongqing 400016, China
    Biosens Bioelectron 25:112-7. 2009
    ..BNEDA) was used as an analytical FRET probe for homogeneous noncompetitive assay of streptavidin (SAV)...
  82. ncbi Plasmonic detection of a model analyte in serum by a gold nanorod sensor
    Stella M Marinakos
    Department of Biomedical Engineering, Center for Biologically Inspired Materials and Material Systems, Duke University, Durham, North Carolina 27708, USA
    Anal Chem 79:5278-83. 2007
    ..b>Streptavidin binding to biotin was monitored by the wavelength shift of the LSPR peak in the UV-vis extinction spectrum of ..
  83. ncbi Synthesis of streptavidin-FITC-conjugated core-shell Fe3O4-Au nanocrystals and their application for the purification of CD4+ lymphocytes
    Hong Ling Liu
    Research Institute of Engineering and Technology, Korea University, Seoul 136 713, Republic of Korea
    Biomaterials 29:4003-11. 2008
    ..b>Streptavidin-fluorescein isothiocyanate (STA-FITC) was conjugated to the surface of the Fe(3)O(4)-Au core-shell nanocrystals ..
  84. ncbi Single-chain Fv-streptavidin substantially improved therapeutic index in multistep targeting directed at disialoganglioside GD2
    Nai Kong V Cheung
    Department of Pediatrics, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    J Nucl Med 45:867-77. 2004
    ..Neuroblastoma is uniquely suited for such investigations because of its abundance of surface ganglioside GD2...
  85. ncbi Internalization of biotinylated compounds into cancer cells is promoted by a molecular Trojan horse based upon core streptavidin and clostridial C2 toxin
    Jörg Fahrer
    Institute of Pharmacology and Toxicology, University of Ulm Medical Center, Albert Einstein Allee 11, 89081 Ulm, Germany
    Naunyn Schmiedebergs Arch Pharmacol 383:263-73. 2011
    ..In the present study, we generated a delivery system building on C2IN and a truncated core streptavidin (Stv13) with enhanced solubility for the C2IIa-dependent internalization of biotinylated cargo molecules into ..
  86. ncbi Cellular uptake of fluorescent labelled biotin-streptavidin microspheres
    M Bradley
    Chemical Biology Section, School of Chemistry, University of Edinburgh, Joseph Black Building, West Mains Road, Edinburgh, UK
    J Fluoresc 18:733-9. 2008
    Amino functionalized, cross-linked, polystyrene microspheres were covalently loaded with streptavidin to which was coupled fluorescently labeled biotin and biotinylated-tagged DNA...
  87. ncbi Position-specific incorporation of dansylated non-natural amino acids into streptavidin by using a four-base codon
    Takahiro Hohsaka
    Department of Bioscience and Biotechnology, Okayama University, 3 1 1 Tsushimanaka, Okayama 700 8530, Japan
    FEBS Lett 560:173-7. 2004
    ..carrying a dansyl fluorescent group were designed, synthesized, and incorporated into various positions of streptavidin by using a CGGG four-base codon in an Escherichia coli in vitro translation system...
  88. ncbi Microspotting streptavidin and double-stranded DNA arrays on gold for high-throughput studies of protein-DNA interactions by surface plasmon resonance microscopy
    Jennifer S Shumaker-Parry
    Department of Chemistry, University of Washington, Seattle, WA 98195 1700, USA
    Anal Chem 76:918-29. 2004
    ..Both methods use streptavidin (SA) as a linker layer between a biotin-containing mixed self-assembled monolayer (SAM) and biotinylated dsDNAs ..
  89. ncbi Synthesis of monodisperse biotinylated p(NIPAAm)-coated iron oxide magnetic nanoparticles and their bioconjugation to streptavidin
    Ravin Narain
    Department of Chemistry and Biochemistry, Laurentian University, Sudbury, ON, P3E 2C6, Canada
    Langmuir 23:6299-304. 2007
    ..The ability of the biotin terminal groups on the b-PNIPAAm-coated nanoparticles to interact with streptavidin was confirmed by fluorescence and surface plasmon resonance...
  90. ncbi The use of streptavidin conjugates as immunoblot loading controls and mitochondrial markers for use with Dictyostelium discoideum
    Andrew J Davidson
    Beatson Institute for Cancer Research, Glasgow, UK
    Biotechniques 55:39-41. 2013
    ..Here we demonstrate the use of labeled streptavidin to detect biotinylated mitochondrial 3-methylcrotonyl-CoA carboxylase α (MCCC1), providing a robust and ..
  91. pmc Pretargeted radioimmunotherapy using genetically engineered antibody-streptavidin fusion proteins for treatment of non-hodgkin lymphoma
    Steven I Park
    Department of Medicine, University of North Carolina, Chapel Hill, USA
    Clin Cancer Res 17:7373-82. 2011
    Pretargeted radioimmunotherapy (PRIT) using streptavidin (SAv)-biotin technology can deliver higher therapeutic doses of radioactivity to tumors than conventional RIT...
  92. pmc RNA affinity tags for purification of RNAs and ribonucleoprotein complexes
    Chatchawan Srisawat
    Department of Biological Chemistry, University of Michigan, 3200 MSRB III, 1150 West Medical Center Drive, Ann Arbor 48109 0606, USA
    Methods 26:156-61. 2002
    ..Here, the preparation and use of two RNA affinity tags against Sephadex or streptavidin are described. The two tags have different strengths that make them appropriate for slightly different uses...
  93. ncbi Tumor delivery of antisense oligomer using trastuzumab within a streptavidin nanoparticle
    Yi Wang
    Division of Nuclear Medicine, Department of Radiology, University of Massachusetts Medical School, Worcester, MA 01655, USA
    Eur J Nucl Med Mol Imaging 36:1977-86. 2009
    ..The objective of this study was to investigate whether trastuzumab can be used as a specific carrier to deliver antisense oligomers into Her2+ tumor cells both in vitro and in vivo...
  94. ncbi A streptavidin-protein cage Janus particle for polarized targeting and modular functionalization
    Peter A Suci
    Center for BioInspired Nanomaterials, Montana State University, Bozeman, Montana 59717, USA
    J Am Chem Soc 131:9164-5. 2009
    ..modification to assemble new types of targeting nanoplatforms by docking the universal coupling protein, streptavidin (StAv), onto a restricted region of the surface of a small protein cage...
  95. ncbi Performance of antibody microarrays fabricated by either DNA-directed immobilization, direct spotting, or streptavidin-biotin attachment: a comparative study
    Ron Wacker
    Chimera Biotec GmbH, Emil Figge Strasse 76A, D 44227 Dortmund, Germany
    Anal Biochem 330:281-7. 2004
    ..DDI is based on the self-assembly of semisynthetic DNA-streptavidin conjugates which converts an array of DNA oligomers into an antibody microarray...
  96. ncbi Endogenous biotin in rat brain: implications for false-positive results with avidin-biotin and streptavidin-biotin techniques
    Bruce E McKay
    Hotchkiss Brain Institute, University of Calgary, Calgary, Alberta, Canada
    Methods Mol Biol 418:111-28. 2008
    The interaction between avidin and biotin or streptavidin and biotin forms the basis of several widely used immunohistochemical techniques...
  97. ncbi Conjugation of the photoluminescent silicon nanoparticles to streptavidin
    Jonghoon Choi
    Department of Chemical and Biomolecular Engineering, University of Maryland, College Park, MD 20742, USA
    Bioconjug Chem 19:680-5. 2008
    We have covalently attached multiple photoluminescent silicon nanoparticles (SNs) to streptavidin molecules. Conjugation of SNs to a target protein is achieved using the multistage photoassisted procedure...
  98. ncbi Detection of IgE antibodies to bacitracin using a commercially available streptavidin-linked solid phase in a patient with anaphylaxis to triple antibiotic ointment
    Saba Sharif
    Kaiser Permanente, Los Angeles Medical Center, Los Angeles, California, USA
    Ann Allergy Asthma Immunol 98:563-6. 2007
    ..Because of the potential for provoking a systemic reaction by skin testing, it would be advantageous to develop an in vitro test for bacitracin specific IgE...
  99. ncbi The precise control of cell labelling with streptavidin paramagnetic particles
    Vincent H B Ho
    Department of Chemical Engineering and Biotechnology, University of Cambridge, New Museums Site, Pembroke St, Cambridge CB2 3RA, UK
    Biomaterials 30:6548-55. 2009
    ..provides a quick way of labelling cells by first biotinylating the cell membrane proteins and then binding streptavidin paramagnetic particles onto the biotinylated proteins...
  100. ncbi Detection of protein carbonyls by means of biotin hydrazide-streptavidin affinity methods
    Kenneth Hensley
    Free Radical Biology and Aging Research Program, Oklahoma Medical Research Foundation, Oklahoma City, OK, 73104, USA
    Methods Mol Biol 536:457-62. 2009
    ..Biotin hydrazide-labeled proteins are detectable using standard streptavidin-coupled detection techniques such as peroxidase-catalyzed chemiluminescence of immunoblots...
  101. ncbi Biotinylation of TiO(2) nanoparticles and their conjugation with streptavidin
    Lu Ye
    Department of Chemical Engineering, Center for Pulp and Paper Research, and Department of Chemistry, McMaster University, 1280 Main Street, West Hamilton, Ontario, Canada L8S 4L7
    Langmuir 23:5630-7. 2007
    ..It was postulated that TiO(2) particles could be directed to specific targets of interest using biotin/streptavidin linkages...

Research Grants65

  1. MOLECULAR RECOGNITION IN THE STREPTAVIDIN BIOTIN SYSTEM
    Patrick Stayton; Fiscal Year: 2002
    DESCRIPTION (Adapted from abstract): Study of the streptavidin- biotin model system is providing molecular insight into the structure-function relationships, which govern the high affinity equilibrium thermodynamics and the construction ..
  2. Bone Marrow Transplantation for Hematologic Malignancies using Novel Radioimmunot
    John M Pagel; Fiscal Year: 2013
    ..employing MHC-haploidentical stem cells utilizing myeloablative pretargeted RIT with an anti-CD45 Ab (30F11)-streptavidin (SA) conjugate followed by either 90Y- or 177Lu-labeled DOTA-biotin (as determined from aim 1 the best ..
  3. Characterizing gene regulation with single molecule sensitive probes
    Philip J Santangelo; Fiscal Year: 2013
    ..resistant, linear nucleic acids, labeled with multiple, high quantum-yield fluorophores linked together by streptavidin, via the biotin-streptavidin linkage...
  4. Development of CNS-targeted AAV vectors
    MIGUEL S ESTEVES; Fiscal Year: 2012
    ..As brain-targeting molecules we will use Streptavidin fused to a single-chain antibody specific for the mouse Transferrin receptor (TfR), or peptides derived from ..
  5. P63/IGFBP3/BCL2 Control of Corneal Epithelial Homeostasis
    Danielle M Robertson; Fiscal Year: 2011
    ..membrane localization and subcellular trafficking of IGFBP3 (cell fractionation and WB, and biotinylated IGFPB3-streptavidin-conjugated quantum dots using time-lapse confocal microscopy);and characterize the role of dimerization and ..
  6. Affinity Tagging of Adipocyte Nuclei to Examine Epigenetic Controls in Obesity
    Richard Brian Meagher; Fiscal Year: 2013
    ..of normal or environmentally compromised mice or obese mice may then be purified from mixed tissue samples on Streptavidin magnetic affinity resin...
  7. Genetic hearing screening for newborns
    Xi Lin; Fiscal Year: 2009
    ..We will test a device based on biotinylated cDNA bait probes fixed on streptavidin- coated magnetic microspheres, which can be manufactured in-house without the use of any specialized equipment...
  8. Radioimmunotherapy for Multiple Myeloma
    Damian J Green; Fiscal Year: 2013
    ..anti-CD38 (OKT10) monoclonal antibody and suggest that an improved therapeutic index will result with anti-CD38 streptavidin (SA) fusion protein (OKT10[scFv]4SA) PRIT...
  9. Defining the thyroid hormone receptor Cistrome in vivo
    Anthony N Hollenberg; Fiscal Year: 2012
    ..The biotinylated TR21 isoform can then be precipitated in ChIP by streptavidin and the recovered DNA subjected to deep parallel sequencing (ChIP-seq) to define the TR cistrome in target ..
  10. Targeted Delivery of a Novel Synthetic Curcumin Analog, EF24 to Glioblastoma and
    MAMORU NONE SHOJI; Fiscal Year: 2012
    ..5-labeled EF24-FFRck- fVIIa. The distribution of the drug using biotinylated EF24 combined with streptavidin histochemistry will be used to more precisely localize the drug in glioma xenografts tissue sections...
  11. CD45 Pretargeted Radioimmunotherapy for AML
    Oliver W Press; Fiscal Year: 2013
    ..of conventional RIT using a directly radiolabeled anti-mCD45 Ab (30F11) with pretargeted RIT using a 30F11-streptavidin (SA) conjugate, followed by 131I, 177Lu or 90Y-labeled DOTA-biotin...
  12. Dengue diagnostics using a novel peroxidase chain reaction (PxCR)
    PETER PETER ZASSENHAUS; Fiscal Year: 2013
    ..In PxCR, targets are captured onto a biosensor, tagged with a biotinylated probe, and then coated with streptavidin-conjugated horseradish peroxidase (SA-Px)...
  13. Bispecific Antibody Engineering for AML RIT
    Oliver W Press; Fiscal Year: 2013
    ..anti-CD45 x anti- ligand bispecific Abs (scFv2-iFabs) with our current gold standard method of PRIT using a streptavidin-biotin pretargeting method...
  14. Interplay between Chromatin and Co-activator Complexes
    Michael F Carey; Fiscal Year: 2012
    ..The templates are attached to streptavidin-coated beads to capture the PICs from extracts and analyze their composition by immunoblotting...
  15. REGULATION OF MEMBRANE EXCITABILITY
    Kurt G Beam; Fiscal Year: 2012
    ..Aim 1 is to use site-directed binding of streptavidin and FRET to identify conformational changes of the DHPR that are essential for EC coupling...
  16. THERAPY OF LYMPHOMA/LEUKEMIA WITH MONOCLONAL ANTIBODIES
    Oliver W Press; Fiscal Year: 2013
    ..and in normal organs following targeting with directly radiolabeled 111ln-BC8 antibody or with pretargeted BC8-streptavidin followed by 111In-DOTA-biotin...
  17. Functional proteomics in differentiating erythroid cells
    IOANNIS JOHN STROUBOULIS; Fiscal Year: 2013
    ..Biotinylated transcription factors will be isolated from cells using streptavidin coated magnetic beads and subjected to mass-spectrometry for identifying associated proteins, to chromatin ..
  18. CD38 Pretargeted Radioimmunotherapy for Myeloma
    Oliver W Press; Fiscal Year: 2013
    ..radiolabeled anti-CD38 Ab (OKT10) with the biodistribution of PRIT using an engineered tetravalent OKT10 scFv4-streptavidin (SA) molecular fusion protein, followed by a dendrimeric "clearing agent" and then 90Y-labeled DOTA-biotin...
  19. Critical Role of Mybl2 in Spontaneous and Short-Chain Fatty Acid-Induced Intestin
    MICHAEL JOSEPH PAPETTI; Fiscal Year: 2011
    ..Functional targets will then be immobilized on streptavidin/agarose to physically capture miRNAs (known or novel) from small RNAs of differentiating colonic epithelial ..
  20. Novel Approaches to Access GPIs and GPI-Anchored Proteins for the Study of GPI An
    Zhongwu Guo; Fiscal Year: 2012
    ..Thereafter, GPI-anchored proteins will be easily isolated with a streptavidin column and analyzed by MS and other methods established for proteomic studies...
  21. Epigenomic profiling of histone turnover kinetics in mammalian cells
    Steven Henikoff; Fiscal Year: 2013
    ..with a biotin moiety, selective extraction of nucleosome core particles, affinity purification with streptavidin, and extraction of DNA for genome-wide profiling...
  22. Label-free HIV-1 Viral Load Measurement through a Contactless Electrode Microchip
    Hadi Shafiee; Fiscal Year: 2013
    ..b>Streptavidin-coated nano-magnetic beads conjugated with gp120 antibody will be used to selectively capture and isolate HIV-1 ..
  23. Triplex-based Aptamers to Detect DNA Damage
    Paul S Miller; Fiscal Year: 2010
    ..or 8-oxo-dA;ligand induced triplex formation will displace the probe;ligand-free aptamers will be captured on streptavidin beads;and ligand- bound aptamers will be isolated and sequenced...
  24. TRANSLATIONAL CONTROL DURING MURINE SPERMATOGENESIS
    Robert E Braun; Fiscal Year: 2010
    ..Prm1-enriched mRNPs will be isolated using biotinylated antisense RNAs and streptavidin-coated immunomagnetic beads...
  25. Quantitative molecular profiling of tumor biomarkers with multi-color monovalent
    Hongyan Liu; Fiscal Year: 2012
    ..Conjugates with one copy of streptavidin per QD and monovalent QD will be separated through the hybrid gel and recovered using a lab-made eluter device...
  26. Enhanced Oral Tissue Repair via Self Assembled Polymer Multi-layer Barriers for t
    THOMAS DEAN DZIUBLA; Fiscal Year: 2010
    ..These polymers will use fibrin targeting via peptide modification, and biotin-streptavidin binding for layer by layer assembly...
  27. Tumor exosomes as agents of genetic change
    XANDRA OWENS BREAKEFIELD; Fiscal Year: 2011
    ..biotinylated-imaging agents (for diagnostics) or biotinylated-diphtheria toxin (for therapy) complexed to streptavidin which binds with extremely high affinity to biotin (Kd = 10-15) and can be internalized into tumor cells...
  28. Multivalent Nanophage Engineered as Dual Receptor Cancer Theranostic Agents.
    Susan L Deutscher; Fiscal Year: 2013
    ..Following phage clearance, fluorescent streptavidin-coated silica nanoparticles will be used to target the multiple biotins on tumor-bound phage...
  29. Metabolic Engineering with Bioorthogonal Chemical Reporters
    Carolyn R Bertozzi; Fiscal Year: 2013
    ..N-azidoacetylmannosamine (ManNAz) followed by sequential injection of biotinylated phosphine and fluorescent streptavidin conjugates...
  30. Anionic Phospholipid-Selective PET/SPECT Agent for Infection Imaging
    BRIAN DAVID GRAY; Fiscal Year: 2012
    ..In an alternative, non-covalent radiolabeling approach, we will use streptavidin (SA) as a linker between the biotinylated Zn-DPA targeting motif and a biotinylated chelator to form an imaging ..
  31. Targeted Inhibition of Innate Immunosurveillance to Promote the Engraftment of He
    NATHANIEL FERNHOFF; Fiscal Year: 2013
    ..Using biotin- streptavidin crosslinking, we have successfully increased cell surface display of CD47 and we are actively characterizing ..
  32. Nanoparticle Induced Circuit Excitation
    Jeffrey M Friedman; Fiscal Year: 2013
    ..Ferrous oxide coated with streptavidin is used to decorate cells, which express a biotin acceptor protein under the control of cell specific promoters...
  33. Artificial Metalloproteins: Confining Metal Complexes within Protein Hosts. (Orig
    Andrew S Borovik; Fiscal Year: 2013
    ..Protein hosts streptavidin and avidin serve as new binding sites for synthetic metal complexes: these proteins do not normally bind metal ..
  34. Single cell analysis of chromatin changes at individual genes
    Oleg N Denisenko; Fiscal Year: 2013
    ..In this method, fluorogenic substrate conjugated to streptavidin is tethered to a genomic locus of interest via biotinylated DNA probe;and the cognate enzyme is recruited to a ..
  35. PRETARGETED RADIOIMMUNOTHERAPY OF CD20+ LYMPHOMAS
    Oliver W Press; Fiscal Year: 2012
    ..Two separate pretargeting approaches will be investigated, one using streptavidin (SA) and radioactive biotin and the second employing molecularly engineered bispecific anti-CD20 x anti-ligand ..
  36. Development of At-211-Labeled Reagents for Therapy of Metastatic Prostate Cancer
    DANIEL SCOTT WILBUR; Fiscal Year: 2013
    ..Those trifunctional molecules will be conjugated with recombinant streptavidin (rSAv) and that conjugate will be succinylated to demonstrate cancer targeting in that model system...
  37. Complexes of HIV-1 Nef with Src-family Kinases as Therapeutic Targets
    Lori A Emert Sedlak; Fiscal Year: 2010
    ..This compound represents the first to target a viral protein:host cell protein kinase complex, providing a new lead for anti-HIV drug development. ..
  38. Global protein palmitoylation and DHHC proteins in T cell activation and anergy
    Amnon Altman; Fiscal Year: 2013
    ..heavy and light membrane proteomes will be mixed and coupled to biotin-azide using click chemistry, enriched on streptavidin-agarose beads, trypsinized, and analyzed using multidimensional protein identification technology (MudPIT) on a ..
  39. Neuronal-specific cargo-delivery platforms as post-exposure botulism therapies
    Brenda A Wilson; Fiscal Year: 2013
    ..Peptide/protein-based inhibitory cargo-delivery vehicles, and Aim 2. Biotin-streptavidin-linked inhibitory cargos-delivery vehicles...
  40. The role of the DOC2.1 protein in Toxoplasma gondii Ca2+- dependent exocytosis
    Marc Jan Gubbels; Fiscal Year: 2013
    ..in promiscuous biotinilyation of proteins in the same complex, which subsequently can be easily identified by streptavidin enrichment and mass spectrometry. Putative TgDOC2...
  41. The Structural Basis of Eukaryotic Replication Origin Licensing by Cryo-EM
    Huilin Li; Fiscal Year: 2010
    ..The origin DNA fragments will be biotinylated and labeled with streptavidin before binding with ORC and Cdc6p for EM analysis...
  42. TOPIC 319 TECHNOLOGY TO GENERATE ANTI PEPTIDE CAPTUE REAGENTS FOR AFFINITY ENRIFH
    John Bruno; Fiscal Year: 2013
    ..These aptamers will be biotinylated and coupled to streptavidin-coated magnetic microbeads (MBs) or covalently bound to MBs, if necessary...
  43. X-LINKED OCULAR ALBINISM & RETINITIS PIGMENTOSA
    Anand Swaroop; Fiscal Year: 1993
    ..biotinylated complementary RNA from human B-lymphocytes, and by subsequently separating the specific cDNAs by streptavidin-agarose chromatography...
  44. Growth Factor Coated Sutures for Improved Tendon Repair
    HANNE GRON; Fiscal Year: 2010
    ..these sequences, a conjugation strategy, attaching suture- and GDF7-binding peptides through a biotin:streptavidin system, was used to test their potential efficacy as a bifunctional linker molecule...
  45. ApoVax-SVN as a Novel Vaccine for Cancer Immunotherapy
    Haval Shirwan; Fiscal Year: 2007
    ..ProtEx(tm) involves the generation of chimeric immunological ligands with a modified form of core streptavidin, modification of the cell membrane with biotin, and decoration with chimeric proteins...
  46. Superior Near-IR Dyes for Bioimaging
    ARUNKUMAR EASWARAN; Fiscal Year: 2009
    ..be followed by conjugation of the squaraine rotaxane probes as well as Cy5 as control probe to IgG protein, streptavidin and a folic acid derivative. 2) SA2 to be perfomed at UND - In vitro and in vivo evaluation of bioconjugates...
  47. ApoVax104-HPV as a Novel Vaccine for Cervical Cancer
    KATHRYN MACLEOD; Fiscal Year: 2009
    ..i) a chimeric molecule containing the extracellular domain of costimulatory 4-1BBL fused C-terminus to core streptavidin (ApoVax104), and ii) biotinylated HPV 16 E7 oncoprotein as a TAA conjugated to the chimeric protein via biotin/..
  48. MOLECULAR RECOGNITION IN THE STREPTAVIDIN BIOTIN SYSTEM
    Patrick Stayton; Fiscal Year: 2001
    DESCRIPTION (Adapted from abstract): Study of the streptavidin- biotin model system is providing molecular insight into the structure-function relationships, which govern the high affinity equilibrium thermodynamics and the construction ..
  49. Bright and Stable Near-IR dyes for Bioimaging
    ARUNKUMAR EASWARAN; Fiscal Year: 2009
    ..by conjugation of the squaraine rotaxane probes as well as Cy7 as a control probe to Zn-DPA, IgG protein and streptavidin . 2) SA2 to be perfomed at UND - In vitro evaluation of bioconjugates...
  50. BIOSPECIFIC POLYMER ENZYME CONJUGATES FOR DRUG DELIVERY
    GLEN KWON; Fiscal Year: 2004
    ..A biotin moiety may tether an antibody in conjunction with biotin and streptavidin. Lastly, a biotin moiety may bind a clearing agent (e.g...
  51. PATHOGENESIS AND PREVENTION OF IDDM
    Hugh McDevitt; Fiscal Year: 2002
    ..by producing soluble biotinylated I-A/g7 molecules with a covalently bound peptide coupled to fluorescent streptavidin. The peptide epitopes of glutamic acid decarboxylase and pre pro-insulin will be identified in Dr...
  52. Aminoglycoside Antivirals to Combat Arenaviruses
    JUAN DE LA TORRE; Fiscal Year: 2004
    ..For this, biotin-RNA conjugates of the LCMV promoter will be attached to streptavidin-coated sensor chips and used as a ligand to screen the libraries using surface plasmon resonance...
  53. In-focus Phase Contrast for Cryo-EM
    Robert M Glaeser; Fiscal Year: 2013
    ..these devices will be determined from cryo-EM images of biological test specimens such as monolayer crystals of streptavidin and tobacco mosaic virus...
  54. ApoFasL: a Novel Immunotherapeutic for T1D
    Rolf Huseby; Fiscal Year: 2007
    ..ProtEx(tm) involves the generation of chimeric immunological ligands with a modified form of core streptavidin, modification of a cell membrane with biotin, and decoration of biotinylated membrane with chimeric proteins...
  55. BIOLUMINESCENT PROTEINS AS LABELS IN DIAGNOSTIC ASSAYS
    MILTON CORMIER; Fiscal Year: 1992
    ..Aim #2 Apoaequorin, aequorin and luciferase will be covalently coupled to streptavidin and/or avidin to prepared conjugates...
  56. Multiplex bead-PCR diagnosis for AIDS diarrhea
    Eric Houpt; Fiscal Year: 2007
    ..Fluorescence is generated by addition of streptavidin-PE and quantitated per bead color on a Luminex suspension array machine, such that up to 100 pathogen species ..
  57. CONFORMATIONAL CHANGES ASSOCIATED WITH K+ CHANNEL GATING
    ROBERT BLAUSTEIN; Fiscal Year: 2000
    ..electrophysiologic recording, oocytes with open channels will be exposed to a biotinylating reagent and then to streptavidin, a large molecule that binds tightly to biotin...
  58. IMPROVED SECOND ANTIBODY RADIOIMMUNOTHERAPY OF CANCER
    ROBERT SHARKEY; Fiscal Year: 2000
    ..Preclinical studies will continue to evaluate the avidin (or streptavidin) biotin pretargeting methodology (A-B method)...
  59. PHASE I STUDY OF ANTICD20/STREPTAVIDIN
    Hazel Breitz; Fiscal Year: 2001
    ..applicant's abstract): The objective of this proposal is to test whether a genetically engineered anti-CD20/streptavidin fusion protein can be used for the initial pretargeting step in the treatment of non-Hodgkin' lymphoma using a ..
  60. Chimeric CD80-SA as a Novel Cancer Vaccine
    Rolf Huseby; Fiscal Year: 2006
    ..ProtEx involves the generation of chimeric proteins with core streptavidin, biotinylation of the cell surface, and decoration with chimeric proteins...
  61. Reagents to Investigate Glycosylated Macromolecules
    Animesh Nandi; Fiscal Year: 2004
    ..labeled with peracetylated N-(2-azidoacetyl) glucosamine (peracetylated GIcNAc azide) and can be detected by streptavidin HRP following conjugation with a biotinylated phosphine capture reagent...
  62. MONOCLONAL ANTIBODIES FOR IN VIVO LABELED BLOOD PRODUCTS
    Mary Rusckowski; Fiscal Year: 1992
    ..The direction of the project is to couple the antibody to avidin or streptavidin. The antibody-avidin complex will be injected, allowed to localize at specific sites and the excess to clear ..
  63. Engineered Macromolecule for Controlled Synthesis in Nanotechnology
    Patricia Weber; Fiscal Year: 2007
    unreadable] DESCRIPTION (provided by applicant): Our objective is to develop a Streptavidin Macromolecular Adaptor (SAMA) enabling the controlled assembly of streptavidin-linked protein conjugates for nanotechnology, biomaterials and ..
  64. Engineered Proteins for Nanotechnology Applications
    Patricia Weber; Fiscal Year: 2007
    ..Our initial strut molecule is streptavidin, a tetrameric protein molecule of MW 60,000 with D2 symmetry that has 4 high-affinity binding sites for the ..
  65. STRUCTURE/FUNCTION IN IMMOBILIZED PROTEIN FILMS
    STEVEN SAAVEDRA; Fiscal Year: 1999
    ..b>Streptavidin will form the inner layer of bilayer films; monolayer films will serve as controls for bilayer films...