integration host factors

Summary

Summary: Bacterial proteins that are used by BACTERIOPHAGES to incorporate their DNA into the DNA of the "host" bacteria. They are DNA-binding proteins that function in genetic recombination as well as in transcriptional and translational regulation.

Top Publications

  1. pmc Growth phase-dependent variation in protein composition of the Escherichia coli nucleoid
    T Ali Azam
    Department of Molecular Genetics, National Institute of Genetics, Mishima, Shizuoka 411 8540, Japan
    J Bacteriol 181:6361-70. 1999
  2. ncbi IHF and HU: flexible architects of bent DNA
    Kerren K Swinger
    Department of Biochemistry and Molecular Biology, University of Chicago, Chicago, IL 60637, USA
    Curr Opin Struct Biol 14:28-35. 2004
  3. ncbi A divalent metal-mediated switch controlling protein-induced DNA bending
    Qiuye Bao
    Division of Genomics and Genetics, School of Biological Sciences, Nanyang Technological University, Singapore 637551, Singapore
    J Mol Biol 367:731-40. 2007
  4. ncbi Integration host factor is involved in transcriptional regulation of the Brucella abortus virB operon
    Rodrigo Sieira
    Instituto de Investigaciones Biotecnologicas, Universidad Nacional de General San Martin, CONICET, San Martin 1650, Buenos Aires, Argentina
    Mol Microbiol 54:808-22. 2004
  5. pmc Association of nucleoid proteins with coding and non-coding segments of the Escherichia coli genome
    David C Grainger
    School of Biosciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK
    Nucleic Acids Res 34:4642-52. 2006
  6. pmc Searching for and predicting the activity of sites for DNA binding proteins: compilation and analysis of the binding sites for Escherichia coli integration host factor (IHF)
    J A Goodrich
    Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, PA 15213
    Nucleic Acids Res 18:4993-5000. 1990
  7. ncbi Escherichia coli prereplication complex assembly is regulated by dynamic interplay among Fis, IHF and DnaA
    Valorie T Ryan
    Department of Biological Sciences, Florida Institute of Technology, Melbourne, Florida 32901 6795, USA
    Mol Microbiol 51:1347-59. 2004
  8. ncbi Genome organisation and chromatin structure in Escherichia coli
    D Ussery
    Center for Biological Sequence Analysis, Department of Biotechnology, Building 208, The Technical University of Denmark, 2800 Kgs Lyngby, Denmark
    Biochimie 83:201-12. 2001
  9. ncbi Role of upstream activation sequences and integration host factor in transcriptional activation by the constitutively active prokaryotic enhancer-binding protein PspF
    J Dworkin
    Rockefeller University, 1230 York Avenue, New York, NY 10021, USA
    J Mol Biol 273:377-88. 1997
  10. ncbi Crystal structure of an IHF-DNA complex: a protein-induced DNA U-turn
    P A Rice
    Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
    Cell 87:1295-306. 1996

Detail Information

Publications246 found, 100 shown here

  1. pmc Growth phase-dependent variation in protein composition of the Escherichia coli nucleoid
    T Ali Azam
    Department of Molecular Genetics, National Institute of Genetics, Mishima, Shizuoka 411 8540, Japan
    J Bacteriol 181:6361-70. 1999
    ..These changes in the composition of nucleoid-associated proteins in the stationary phase are accompanied by compaction of the genome DNA and silencing of the genome functions...
  2. ncbi IHF and HU: flexible architects of bent DNA
    Kerren K Swinger
    Department of Biochemistry and Molecular Biology, University of Chicago, Chicago, IL 60637, USA
    Curr Opin Struct Biol 14:28-35. 2004
    ..These techniques include time-resolved synchrotron X-ray footprinting, differential scanning calorimetry, isothermal titration calorimetry and single-molecule experiments...
  3. ncbi A divalent metal-mediated switch controlling protein-induced DNA bending
    Qiuye Bao
    Division of Genomics and Genetics, School of Biological Sciences, Nanyang Technological University, Singapore 637551, Singapore
    J Mol Biol 367:731-40. 2007
    ..Introduction of acidic residues at the protein-DNA interface holds potential for the design of metal-mediated switches for the investigation of functional relationships...
  4. ncbi Integration host factor is involved in transcriptional regulation of the Brucella abortus virB operon
    Rodrigo Sieira
    Instituto de Investigaciones Biotecnologicas, Universidad Nacional de General San Martin, CONICET, San Martin 1650, Buenos Aires, Argentina
    Mol Microbiol 54:808-22. 2004
    ..These data indicate that IHF plays a key role during intracellular virB operon expression being required for the biogenesis of the endoplasmic reticulum-derived replicative vacuole...
  5. pmc Association of nucleoid proteins with coding and non-coding segments of the Escherichia coli genome
    David C Grainger
    School of Biosciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK
    Nucleic Acids Res 34:4642-52. 2006
    ..Hence some targets are associated with combinations of bound FIS, H-NS and IHF. In addition, many regions associated with FIS and H-NS are also associated with RNA polymerase...
  6. pmc Searching for and predicting the activity of sites for DNA binding proteins: compilation and analysis of the binding sites for Escherichia coli integration host factor (IHF)
    J A Goodrich
    Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, PA 15213
    Nucleic Acids Res 18:4993-5000. 1990
    ..The MacTargsearch program and its potential usefulness in searching for other sites and predicting their relative activities is discussed...
  7. ncbi Escherichia coli prereplication complex assembly is regulated by dynamic interplay among Fis, IHF and DnaA
    Valorie T Ryan
    Department of Biological Sciences, Florida Institute of Technology, Melbourne, Florida 32901 6795, USA
    Mol Microbiol 51:1347-59. 2004
    ....
  8. ncbi Genome organisation and chromatin structure in Escherichia coli
    D Ussery
    Center for Biological Sequence Analysis, Department of Biotechnology, Building 208, The Technical University of Denmark, 2800 Kgs Lyngby, Denmark
    Biochimie 83:201-12. 2001
    ..We also show that the regions upstream of genes regulated by H-NS are more curved and have a higher AT content than regions upstream of other genes. These regions in general would also be localised near the replication terminus...
  9. ncbi Role of upstream activation sequences and integration host factor in transcriptional activation by the constitutively active prokaryotic enhancer-binding protein PspF
    J Dworkin
    Rockefeller University, 1230 York Avenue, New York, NY 10021, USA
    J Mol Biol 273:377-88. 1997
    ..These data, taken together, support the model that a precise promoter geometry is necessary for IHF to positively regulate transcription and that IHF may act to prevent activation from inappropriately spaced upstream sites...
  10. ncbi Crystal structure of an IHF-DNA complex: a protein-induced DNA U-turn
    P A Rice
    Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
    Cell 87:1295-306. 1996
    ..One such readout involves a six-base A tract, providing evidence for the importance of a narrow minor groove...
  11. ncbi Biofilms can be dispersed by focusing the immune system on a common family of bacterial nucleoid-associated proteins
    S D Goodman
    Division of Biomedical Sciences, Herman Ostrow School of Dentistry, University of Southern California, Los Angeles, California, USA
    Mucosal Immunol 4:625-37. 2011
    ..We discuss the prospects for targeting DNABII family members as a potential universal strategy for treating biofilm diseases...
  12. pmc Compaction of single DNA molecules induced by binding of integration host factor (IHF)
    B M Ali
    Department of Physics of Complex Systems, The Weizmann Institute of Science, Rehovot 76100, Israel
    Proc Natl Acad Sci U S A 98:10658-63. 2001
    ..Our findings support the long-held view that IHF and other histone-like proteins play an important role in shaping the long-scale structure of the bacterial nucleoid...
  13. ncbi The interaction of E. coli IHF protein with its specific binding sites
    C C Yang
    Laboratory of Molecular Biology, National Institute of Mental Health, Bethesda, Maryland 20892
    Cell 57:869-80. 1989
    ....
  14. ncbi Specific and non-specific interactions of integration host factor with DNA: thermodynamic evidence for disruption of multiple IHF surface salt-bridges coupled to DNA binding
    J A Holbrook
    Department of Biochemistry, University of Wisconsin-Madison, WI 53706, USA
    J Mol Biol 310:379-401. 2001
    ....
  15. ncbi Integration host factor is essential for the optimal expression of the styABCD operon in Pseudomonas fluorescens ST
    Pedro Miguel Santos
    Department of Biology, Third University of Rome, Italy
    Res Microbiol 153:527-36. 2002
    ....
  16. ncbi The specific binding of Escherichia coli integration host factor involves both major and minor grooves of DNA
    S Wang
    Department of Microbiology, University of Illinois, Urbana 61801, USA
    Biochemistry 34:13082-90. 1995
    ..Thus, the specific interaction of IHF with its H' site likely involves interactions with both the minor and major grooves of the DNA...
  17. pmc Targeting bacterial integration host factor to disrupt biofilms associated with cystic fibrosis
    Jodi E Gustave
    Nationwide Children s Hospital, Columbus, OH 43205, USA
    J Cyst Fibros 12:384-9. 2013
    ....
  18. ncbi IHF and HU stimulate assembly of pre-replication complexes at Escherichia coli oriC by two different mechanisms
    Valorie T Ryan
    Department of Biological Services, Florida Institute of Technology, 150 W University Blvd, Melbourne, Florida 32901, USA
    Mol Microbiol 46:113-24. 2002
    ..HU did not redistribute DnaA, but suppressed binding specifically at I3. These results suggest that different pathways mediated by bacterial chromatin proteins exist to regulate pre-RC assembly and unwind oriC...
  19. pmc Aberrant community architecture and attenuated persistence of uropathogenic Escherichia coli in the absence of individual IHF subunits
    Sheryl S Justice
    Center for Microbial Pathogenesis, The Research Institute at Nationwide Children s Hospital, Columbus, Ohio, United States of America
    PLoS ONE 7:e48349. 2012
    ....
  20. pmc Regulation of the Escherichia coli K5 capsule gene cluster: evidence for the roles of H-NS, BipA, and integration host factor in regulation of group 2 capsule gene clusters in pathogenic E. coli
    S Rowe
    School of Biological Sciences, University of Manchester, Manchester M13 9PT, United Kingdom
    J Bacteriol 182:2741-5. 2000
    ..These results indicate that a complex regulatory network involving a number of global regulators exists for the control of expression of group 2 capsules in E. coli...
  21. pmc The isolation and characterization of mutants of the integration host factor (IHF) of Escherichia coli with altered, expanded DNA-binding specificities
    E C Lee
    Department of Microbiology, University of Illinois, Urbana 61801
    EMBO J 11:305-13. 1992
    ..We discuss the possible mechanisms of suppression by the mutants in terms of a model of the IHF-DNA complex proposed by Yang and Nash [Cell, 57, 869-880 (1989)]...
  22. ncbi Integration host factor (IHF) dictates the structure of polyamine-DNA condensates: implications for the role of IHF in the compaction of bacterial chromatin
    Tumpa Sarkar
    School of Chemistry and Biochemistry, Petit Institute of Bioengineering and Bioscience, and School of Biology, Georgia Institute of Technology, Atlanta, Georgia 30332, USA
    Biochemistry 48:667-75. 2009
    ..We propose that a common function of IHF and HU in bacterial cells is to facilitate DNA organization in the nucleoid by the introduction of sharp bends in chromosomal DNA...
  23. ncbi Two types of localization of the DNA-binding proteins within the Escherichia coli nucleoid
    T A Azam
    Department of Molecular Genetics, National Institute of Genetics, Mishima, Shizuoka 411 8540, Japan
    Genes Cells 5:613-26. 2000
    ..The intracellular localization of these proteins in E. coli could be predicted from these data, but no attempt has been made thus far to directly observe the intracellular distribution of the DNA-binding proteins...
  24. pmc Direct interaction between quorum-sensing regulator SmcR and RNA polymerase is mediated by integration host factor to activate vvpE encoding elastase in Vibrio vulnificus
    Hye Sook Jeong
    National Research Laboratory of Molecular Microbiology and Toxicology, Department of Agricultural Biotechnology, Center for Agricultural Biomaterials, and Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul 151 921, South Korea
    J Biol Chem 285:9357-66. 2010
    ..Collectively, the results proposed a model whereby IHF positions SmcR to contact RNAP by looping the vvpE regulatory DNA, thus allowing precise control of the expression level of VvpE during the pathogenesis of V. vulnificus...
  25. ncbi Mapping the transition state for DNA bending by IHF
    Paula Vivas
    Department of Physics M C 273, University of Illinois at Chicago, 845 West Taylor Street, Chicago, IL 60607, USA
    J Mol Biol 418:300-15. 2012
    ....
  26. ncbi From IHF protein to design and synthesis of a sequence-specific DNA bending peptide
    Eduard K Liebler
    Institut fur Organische und Biomolekulare Chemie, Universitat Gottingen, Tammannstrasse 2, D 37077 Gottingen, Germany
    Org Lett 6:2893-6. 2004
    ..The latter was designed for specific DNA recognition in the minor groove followed by bending of the double strand...
  27. ncbi New insights into the transition pathway from nonspecific to specific complex of DNA with Escherichia coli integration host factor
    Paula Vivas
    Department of Physics M C 273, and Department of Bioengineering M C 063, University of Illinois at Chicago, 845 West Taylor Street, Chicago, Illinois 60607, USA
    J Phys Chem B 112:5997-6007. 2008
    ....
  28. ncbi Primary structure of the hip gene of Escherichia coli and of its product, the beta subunit of integration host factor
    E L Flamm
    J Mol Biol 183:117-28. 1985
    ..A hip missense mutation that replaces a completely conserved glycine with aspartate has a null phenotype, suggesting that the conserved regions are functionally important...
  29. ncbi Integration host factor interactions with Neisseria gene sequences: correlation between predicted binding sites and in vitro binding of Neisseria -derived IHF protein
    S A Hill
    Department of Biological Sciences, Northern Illinois University, DeKalb, IL 60115, US
    Mol Cell Probes 16:153-8. 2002
    ..The results show a positive correlation between the identification of a predicted Neisseria IHF binding site and in vitro binding of Neisseria -derived IHF protein...
  30. ncbi Opening of the replication origin of Escherichia coli by DnaA protein with protein HU or IHF
    D S Hwang
    Department of Biochemistry, Stanford University School of Medicine, California 94305 5307
    J Biol Chem 267:23083-6. 1992
    ..The footprint of the nucleotide-free form of the protein, by contrast, was more extensive and thus, less specific...
  31. ncbi Regulation of HU alpha and HU beta by CRP and FIS in Escherichia coli
    L Claret
    Institute de Biologie Physico Chimique, Laboratoire de Physiologie Bacterienne, Paris, France
    J Mol Biol 263:126-39. 1996
    ..It is rather unexpected that such divergent transcriptional regulation controls the two genes encoding a dimeric protein...
  32. pmc The HimA and HimD subunits of integration host factor can specifically bind to DNA as homodimers
    L Zulianello
    Laboratory of Molecular Genetics, Leiden Institute of Chemistry, Leiden University, The Netherlands
    EMBO J 13:1534-40. 1994
    ..A comparable fusion protein with only the DNA-binding domains of HimD forms less stable complexes, suggesting that sequence-specific contacts between IHF and the ihf consensus are mainly provided by the HimA subunit...
  33. pmc A protein-induced DNA bend increases the specificity of a prokaryotic enhancer-binding protein
    J Dworkin
    Laboratory of Genetics, The Rockefeller University, New York, New York 10021, USA
    Genes Dev 12:894-900. 1998
    ..These opposite effects have the consequence of increasing the specificity of activation of a promoter that is susceptible to regulation by proteins bound to a distal site...
  34. ncbi Making DNA do a U-turn: IHF and related proteins
    P A Rice
    Laboratory of Molecular Biology, National Institute of Digestive, Diabetes and Kidney Diseases, National Institutes of Health, 9000 Rockville Pike, Bethesda, MD 20892 0540, USA
    Curr Opin Struct Biol 7:86-93. 1997
    ..In addition to the crystal structure of IHF bound to DNA, the past year has seen a number of advances in the understanding of the interactions of these proteins with DNA in solution...
  35. pmc Stepwise binding and bending of DNA by Escherichia coli integration host factor
    Sawako Sugimura
    Department of Chemistry, Yale University, P O Box 208107, New Haven, CT 06520, USA
    Proc Natl Acad Sci U S A 103:18510-4. 2006
    ..Our observations on the bending step kinetics are in agreement with results using the temperature-jump kinetic method...
  36. pmc DNA looping generated by DNA bending protein IHF and the two domains of lambda integrase
    L Moitoso de Vargas
    Division of Biology and Medicine, Brown University, Providence, RI 02912
    Science 244:1457-61. 1989
    ..The bivalent DNA binding protein is positioned at high affinity sites and directed, by a DNA bending protein, to interactions with distant lower affinity sites. Assembly of this complex is independent of protein-protein interactions...
  37. pmc Formation of a wrapped DNA-protein interface: experimental characterization and analysis of the large contributions of ions and water to the thermodynamics of binding IHF to H' DNA
    Kirk A Vander Meulen
    Department of Biochemistry, University of Wisconsin, Madison, WI 53706, USA
    J Mol Biol 377:9-27. 2008
    ..7+/-0.4 at constant KCl activity, indicating the net release of ca. 150 H(2)O molecules from anionic surface...
  38. ncbi DNA relaxation-dependent phase biasing of the fim genetic switch in Escherichia coli depends on the interplay of H-NS, IHF and LRP
    Colin P Corcoran
    Department of Microbiology, Moyne Institute of Preventive Medicine, School of Genetics and Microbiology, Trinity College Dublin, Dublin 2, Ireland
    Mol Microbiol 74:1071-82. 2009
    ....
  39. ncbi The integration host factor (IHF) integrates stationary-phase and virulence gene expression in Salmonella enterica serovar Typhimurium
    Michael W Mangan
    Department of Microbiology, Moyne Institute of Preventive Medicine, University of Dublin, Trinity College, Ireland
    Mol Microbiol 59:1831-47. 2006
    ..Although the three mutants showed considerable overlaps in the genes affected by the ihf lesions, the observed patterns were not identical, showing that S. Typhimurium has not one but three overlapping IHF regulons...
  40. ncbi The role of integration host factor in gene expression in Escherichia coli
    M Freundlich
    Department of Biochemistry and Cell Biology, State University of New York, Stony Brook 11794
    Mol Microbiol 6:2557-63. 1992
    ....
  41. ncbi The Fis protein: it's not just for DNA inversion anymore
    S E Finkel
    Department of Biological Chemistry, UCLA School of Medicine 90024 1737
    Mol Microbiol 6:3257-65. 1992
    ..The X-ray crystal structure of Fis has been determined and insights into its mode of DNA binding and mechanisms of action in these disparate systems are being made...
  42. pmc In vivo and in vitro effects of integration host factor at the DmpR-regulated sigma(54)-dependent Po promoter
    C C Sze
    Department of Cell and Molecular Biology, , , Sweden
    J Bacteriol 183:2842-51. 2001
    ..Mechanistic implications are discussed in the context of a model in which IHF binding results in transduction of DNA instability from an A+T-rich region to the melt region of the promoter...
  43. ncbi Transposon-like Correia elements: structure, distribution and genetic exchange between pathogenic Neisseria sp
    Nicolas Buisine
    Department of Biochemistry, University of Oxford, UK
    FEBS Lett 522:52-8. 2002
    ..Phylogenetic analysis suggests that their presence predates the divergence of Neisseria meningitidis and Neisseria gonorrhoeae...
  44. pmc The Escherichia coli Fis protein prevents initiation of DNA replication from oriC in vitro
    S Wold
    Department of Biophysics, Institute for Cancer Research, Montebello, Oslo, Norway
    Nucleic Acids Res 24:3527-32. 1996
    ..This indicates a negative role for Fis in the regulation of replication initiation...
  45. pmc Reciprocal expression of integration host factor and HU in the developmental cycle and infectivity of Legionella pneumophila
    Michael G Morash
    Department of Microbiology and Immunology, Faculty of Medicine, Dalhousie University, Halifax, Nova Scotia, Canada B3H 4H7
    Appl Environ Microbiol 75:1826-37. 2009
    ..These studies establish IHF and HU as markers of developmental stages and show that IHF function is required for both differentiation and full virulence of L. pneumophila in natural amoebic hosts...
  46. ncbi PspF and IHF bind co-operatively in the psp promoter-regulatory region of Escherichia coli
    G Jovanovic
    Laboratory of Genetics, The Rockefeller University, New York, NY 10021, USA
    Mol Microbiol 25:473-81. 1997
    ..In the absence of IHF, in vivo autoregulation of pspF transcription is lifted and, consequently, PspF production is increased, indicating that IHF enhances PspF binding to the psp enhancer in vivo...
  47. pmc sigma factor selectivity of Escherichia coli RNA polymerase: role for CRP, IHF and lrp transcription factors
    F Colland
    Institut Pasteur, Unité de Physicochimie des Macromolécules Biologiques URA 1773 du CNRS, 75724 Paris, Cedex 15, France
    EMBO J 19:3028-37. 2000
    ..This different ability of the two holoenzymes to interact productively with promoters once assembled in complex nucleoprotein structures may be a crucial factor in generating sigma(S) selectivity in vivo...
  48. pmc Regulation by nucleoid-associated proteins at the Escherichia coli nir operon promoter
    Douglas F Browning
    School of Biosciences, University of Birmingham, Birmingham, B15 2TT, United Kingdom
    J Bacteriol 190:7258-67. 2008
    ..Differences in the upstream IHF and Fis binding sites at the nir promoter in related enteric bacteria fix the level of nir operon expression under anaerobic growth conditions...
  49. pmc Examining the contribution of a dA+dT element to the conformation of Escherichia coli integration host factor-DNA complexes
    L M Hales
    Department of Microbiology, University of Illinois, Urbana, 61801, USA
    Nucleic Acids Res 24:1780-6. 1996
    ..These results indicate the importance of flanking DNA and a dA+dT element in the binding and bending of a site by IHF...
  50. ncbi DNA microloops and microdomains: a general mechanism for transcription activation by torsional transmission
    A Travers
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge, CB2 2QH, England
    J Mol Biol 279:1027-43. 1998
    ..In this process, which we term torsional transmission, a major function of the activator is to act as a local topological homeostat. We argue that the same mechanism may also be employed in site-specific DNA inversion...
  51. pmc Structure and function of the Pseudomonas putida integration host factor
    R Calb
    Department of Molecular Genetics and Biotechnology, Hadassah Medical School, The Hebrew University, Jerusalem, Israel
    J Bacteriol 178:6319-26. 1996
    ..It was further shown that the ihfA P. putida mutant strain carrying the TOL plasmid was defective in the degradation of the aromatic model compound benzyl alcohol, proving the unique role of IHF in xyl operon promoter regulation...
  52. pmc Integration host factor positively regulates virulence gene expression in Vibrio cholerae
    Emily Stonehouse
    Department of Microbiology and Immunology, Dartmouth Medical School, Hanover, NH 03755, USA
    J Bacteriol 190:4736-48. 2008
    ..In addition, disruption of ihfA in an hns or toxT mutant background had no effect on tcpA expression. These results suggest that IHF may function at the tcpA promoter to alleviate H-NS repression...
  53. ncbi Protein surface salt bridges and paths for DNA wrapping
    Ruth M Saecker
    Department of Chemistry, University of Wisconsin Madison, 1101University Avenue, Madison, WI 53706, USA
    Curr Opin Struct Biol 12:311-9. 2002
    ....
  54. pmc Fimbrial phase variation in Escherichia coli: dependence on integration host factor and homologies with other site-specific recombinases
    C J Dorman
    J Bacteriol 169:3840-3. 1987
    ..A mechanism by which IHF may exert control over fimA expression is discussed...
  55. pmc Activation from a distance: roles of Lrp and integration host factor in transcriptional activation of gltBDF
    L Paul
    Biophysics Research Division, University of Michigan, Ann Arbor, Michigan 48109, USA
    J Bacteriol 183:3910-8. 2001
    ..Based on these results, we suggest that IHF plays a crucial architectural role, bringing the distant Lrp complex in close proximity to the promoter-bound RNA polymerase...
  56. pmc Dual role of response regulator StyR in styrene catabolism regulation
    Livia Leoni
    Department of Biology, University Roma Tre, Viale G Marconi 446, 00146 Rome, Italy
    Appl Environ Microbiol 71:5411-9. 2005
    ..We suggest that the cellular levels of phosphorylated StyR, as determined by StyS sensor kinase activity, and the interplay of this molecule with IHF modulate the activity of the promoter in different growth conditions...
  57. ncbi In vivo UV laser footprinting of the Pseudomonas putidasigma 54Pu promoter reveals that integration host factor couples transcriptional activity to growth phase
    Marc Valls
    Department of Microbial Biotechnology, Centro Nacional de Biotecnologia, Consejo Superior de Investigaciones Cientificas, Campus de Cantoblanco, 28049 Madrid, Spain
    J Biol Chem 277:2169-75. 2002
    ..Further, they validate UV laser technology as a suitable tool to visualize nondisruptive alterations of DNA shape in vivo...
  58. ncbi Role of integration host factor in stimulating transcription from the sigma 54-dependent nifH promoter
    E Santero
    Department of Plant Pathology, University of California, Berkeley 94720
    J Mol Biol 227:602-20. 1992
    ....
  59. pmc In vitro selection of integration host factor binding sites
    S D Goodman
    Department of Basic Sciences, University of Southern California School of Dentistry, Los Angeles, California, USA
    J Bacteriol 181:3246-55. 1999
    ..Despite finding individual sequences that varied over 100-fold in affinity for IHF, we found no apparent correlation between affinity and function...
  60. ncbi A good turn for DNA: the structure of integration host factor bound to DNA
    T Ellenberger
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
    Structure 5:153-7. 1997
    ..IHF exerts leverage in the minor groove and wraps DNA around the body of the protein, providing another example of sequence-specific recognition of the minor groove...
  61. ncbi Structural distortions induced by integration host factor (IHF) at the H' site of phage lambda probed by (+)-CC-1065, pluramycin, and KMnO4 and by DNA cyclization studies
    D Sun
    Department of Microbiology, University of Texas at Austin 78712, USA
    Biochemistry 35:10815-27. 1996
    ..These studies suggest that IHF-induced DNA bending is accompanied by the introduction of a DNA node, DNA unwinding, and/or by some other DNA distortion. An enhanced binding and stability of IHF was observed on small circular DNA...
  62. ncbi Effects of integration host factor and DNA supercoiling on transcription from the ilvPG promoter of Escherichia coli
    B S Parekh
    Department of Microbiology and Molecular Genetics, College of Medicine, University of California, Irvine, California 92697, USA
    J Biol Chem 271:20258-64. 1996
    ..Therefore, IHF binding does not activate transcription simply by increasing the local negative supercoiling of the DNA helix in the downstream promoter region or by differential binding to relaxed and supercoiled DNA templates...
  63. pmc Visualization of coupled protein folding and binding in bacteria and purification of the heterodimeric complex
    Haoyong Wang
    New England Biolabs, Inc, 32 Tozer Road, Beverly, MA 01915, USA
    Proc Natl Acad Sci U S A 100:478-83. 2003
    ..Such interaction has been previously shown to occur between the E. coli integration host factors alpha and beta, and between the domains of the general transcriptional coactivator cAMP response element ..
  64. ncbi The regulation of transcription initiation by integration host factor
    N Goosen
    Laboratory of Molecular Genetics, Leiden Institute of Chemistry, Gorlaeus Laboratories, Leiden University, The Netherlands
    Mol Microbiol 16:1-7. 1995
    ..In addition, it summarizes the evidence indicating that IHF can stimulate transcription via a direct interaction with RNA polymerase and explores the possibility that the asymmetry of the IHF protein might reflect such an interaction...
  65. pmc The positive and negative regulation of Tn10 transposition by IHF is mediated by structurally asymmetric transposon arms
    Sven Sewitz
    Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK
    Nucleic Acids Res 31:5868-76. 2003
    ..This suggests that inhibition of target interactions is due to steric hindrance of the target binding site by a single IHF-folded transposon arm...
  66. ncbi Integration host factor: putting a twist on protein-DNA recognition
    Thomas W Lynch
    Department of Biochemistry and Molecular Biology, The University of Chicago, 920 E 58th Street CLSC 221, Chicago, IL 60637, USA
    J Mol Biol 330:493-502. 2003
    ..Comparison of these structures reveals that DNA twist plays a major role in DNA recognition by IHF, and that this geometric parameter is dependent on the dinucleotide step and not on the bound IHF variant...
  67. ncbi Indirect recognition in sequence-specific DNA binding by Escherichia coli integration host factor: the role of DNA deformation energy
    Kimberly A Aeling
    Institute for Genomics and Bioinformatics, Department of Microbiology and Molecular Genetics, School of Medicine, University of California 92697, USA
    J Biol Chem 281:39236-48. 2006
    ....
  68. pmc Conserved response regulator CtrA and IHF binding sites in the alpha-proteobacteria Caulobacter crescentus and Rickettsia prowazekii chromosomal replication origins
    Ann Karen C Brassinga
    Department of Microbiology and Immunology, Dalhousie University, Halifax, Nova Scotia B3H 4H7, Canada
    J Bacteriol 184:5789-99. 2002
    ..Therefore, CtrA and IHF regulatory proteins have similar binding patterns in both replication origins, and we propose that CzcR is a global cell cycle regulator in R. prowazekii...
  69. pmc Pressure dissociation of integration host factor-DNA complexes reveals flexibility-dependent structural variation at the protein-DNA interface
    Donald F Senear
    Department of Molecular Biology and Biochemistry, College of Medicine, University of California, Irvine, CA 92697, USA
    Nucleic Acids Res 35:1761-72. 2007
    ..Biol. 310, 379-401, 2001). We propose that the volume changes reflect differences in hydration that arise from structural variation at IHF-DNA interfaces while the resulting energetic compensation maintains the same net binding energy...
  70. ncbi DNA looping and catalysis; the IHF-folded arm of Tn10 promotes conformational changes and hairpin resolution
    Paul Crellin
    Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, United Kingdom
    Mol Cell 13:537-47. 2004
    ..Further evidence suggests that the molecular mechanism responsible may be mechanical stress in the IHF loop, related to a change in the relative position of the transposase contacts that anchor the loop on either side...
  71. ncbi Expression of the genes coding for the Escherichia coli integration host factor are controlled by growth phase, rpoS, ppGpp and by autoregulation
    M Aviv
    Department of Cellular Biochemistry, Hebrew University Hadassah Medical School, Jerusalem, Israel
    Mol Microbiol 14:1021-31. 1994
    ..An additional IHF-binding site was found upstream of the himD/hip promoter. All three sites show low binding affinity to IHF suggesting that autoregulation can take place only after sufficiently high levels of IHF accumulate in the cell...
  72. pmc HU and IHF, two homologous histone-like proteins of Escherichia coli, form different protein-DNA complexes with short DNA fragments
    E Bonnefoy
    Laboratoire de Physiologie Bacterienne, Institut de Biologie Physico Chimique, Paris, France
    EMBO J 10:687-96. 1991
    ..coli (IHF) form different structures with the same DNA fragments. Moreover, HU seems to enhance the DNA-binding capacity of IHF to a DNA fragment which does not contain its consensus sequence...
  73. ncbi Characterization of a set of integration host factor mutants deficient for DNA binding
    A E Granston
    Laboratory of Molecular Biology, National Institute of Mental Health, Bethesda, MD 20892
    J Mol Biol 234:45-59. 1993
    ..We discuss the positions of the mutant amino acid residues as they relate to a proposed molecular model of an IHF:DNA complex...
  74. ncbi Quantitative distance information on protein-DNA complexes determined in polyacrylamide gels by fluorescence resonance energy transfer
    M Lorenz
    Department of Molecular Biology, Institute for Molecular Biotechnology, Jena, Germany
    Electrophoresis 22:990-8. 2001
    ..The measured Forster transfer efficiency allows us to deduce information on the overall shape and the DNA bending angle in the complex...
  75. ncbi Mechanisms of specific and nonspecific binding of architectural proteins in prokaryotic gene regulation
    James M Benevides
    School of Biological Sciences, University of MissouriKansas City, 5100 Rockhill Road, Kansas City, Missouri 64110 2499, USA
    Biochemistry 47:3855-62. 2008
    ....
  76. ncbi Genetic and biochemical analysis of the integration host factor of Escherichia coli
    G Mengeritsky
    Department of Molecular Genetics, Hebrew University Hadassah Medical School, Jerusalem, Israel
    J Mol Biol 231:646-57. 1993
    ..The binding of IHF to DNA is probably not restricted to one domain, but requires the co-operative participation of a number of regions of the protein...
  77. ncbi Opposed actions of regulatory proteins, DnaA and IciA, in opening the replication origin of Escherichia coli
    D S Hwang
    Department of Biochemistry, Stanford University School of Medicine, California 94305 5307
    J Biol Chem 267:23087-91. 1992
    ..The selective interactions of DnaA and IciA proteins with the 13-mer region appear to be components of the on/off switch that controls initiation of E. coli chromosomal replication...
  78. pmc The global regulator H-NS acts directly on the transpososome to promote Tn10 transposition
    Simon J Wardle
    Department of Biochemistry, University of Western Ontario, London, Ontario N6A 5C1 Canada
    Genes Dev 19:2224-35. 2005
    ..Sensing of environmental changes through H-NS would allow transposition to occur when it is most opportune for both the transposon and the host...
  79. ncbi IscR acts as an activator in response to oxidative stress for the suf operon encoding Fe-S assembly proteins
    Won Sik Yeo
    Laboratory of Molecular Microbiology, School of Biological Sciences, and, Institute of Microbiology, Seoul National University, Seoul 151 742, Korea
    Mol Microbiol 61:206-18. 2006
    ..These results support a model that IscR in its demetallated form directly activates sufA transcription, while it de-represses isc operon, under oxidative stress condition...
  80. ncbi Integration of three signals at the Escherichia coli nrf promoter: a role for Fis protein in catabolite repression
    Douglas F Browning
    School of Biosciences, The University of Birmingham, Birmingham B15 2TT, UK
    Mol Microbiol 57:496-510. 2005
    ..Hence, Fis protein is a major factor responsible for catabolite repression at the nrf promoter, and Fis can override activation by FNR and NarL or NarP...
  81. ncbi A DNA architectural protein couples cellular physiology and DNA topology in Escherichia coli
    R Schneider
    Institut fur Genetik und Mikrobiologie, LMU Munchen, Maria Ward Str 1a, 80638 Munchen, Germany
    Mol Microbiol 34:953-64. 1999
    ..We propose that FIS is involved in coupling cellular physiology to the topology of the bacterial chromosome...
  82. ncbi Growth phase-dependent regulation of nuoA-N expression in Escherichia coli K-12 by the Fis protein: upstream binding sites and bioenergetic significance
    B Wackwitz
    Institut für Mikrobiologie und Weinforschung, Universitat Mainz, Germany
    Mol Gen Genet 262:876-83. 1999
    ..This ensures higher ATP yields under conditions where large amounts of ATP are required...
  83. pmc A role for the umuDC gene products of Escherichia coli in increasing resistance to DNA damage in stationary phase by inhibiting the transition to exponential growth
    S Murli
    Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    J Bacteriol 182:1127-35. 2000
    ..Thus, the umuDC gene products appear to increase DNA damage tolerance at least partially by regulating growth after DNA damage in both exponentially growing and stationary-phase cells...
  84. ncbi The regulation of the Escherichia coli mazEF promoter involves an unusual alternating palindrome
    I Marianovsky
    Department of Cellular Biochemistry, Hebrew University Hadassah Medical School, Ein Kerem, Jerusalem, 91120 Israel
    J Biol Chem 276:5975-84. 2001
    ....
  85. ncbi Involvement of FIS in the H-NS-mediated regulation of virF gene of Shigella and enteroinvasive Escherichia coli
    M Falconi
    Laboratorio di Genetica, Dipartimento di Biologia MCA, , 62032 Camerino (MC, Italy
    Mol Microbiol 42:439-52. 2001
    ..Our data indicate that FIS may be relevant for the rapid increase in virF expression after penetration of bacteria into the host...
  86. ncbi Structural analysis of the transcriptional activation region on Fis: crystal structures of six Fis mutants with different activation properties
    Y S Cheng
    Graduate Institute of Life Science, National Defense Medical Center, Taipei, Taiwan, Republic of China
    J Mol Biol 302:1139-51. 2000
    ..The structures and biochemical properties of the mutants suggest that Arg71 is the most critical residue for contacting RNAP within this ridge and that the glycine at position 72 helps to stabilize the structure...
  87. pmc Involvement of Fis protein in replication of the Escherichia coli chromosome
    M Filutowicz
    Department of Bacteriology, University of Wisconsin Madison 53706
    J Bacteriol 174:398-407. 1992
    ..Finally, purified Fis protein interacts in vitro with sites in oriC. These interactions could be involved in mediating the effect of Fis on DNA synthesis in vivo...
  88. ncbi Control of autogenous activation of Herbaspirillum seropedicae nifA promoter by the IHF protein
    Roseli Wassem
    Departamento de Bioquímica UFPR, Caixa Postal 19046, CEP 81531 990, Curitiba, PR Brazil
    FEMS Microbiol Lett 212:177-82. 2002
    ..IHF is apparently required to prevent overexpression of the NifA protein via auto-activation under nitrogen-fixing conditions in H. seropedicae...
  89. ncbi The Pseudomonas aeruginosa hemA promoter is regulated by Anr, Dnr, NarL and Integration Host Factor
    R Krieger
    Institut fur Organische Chemie und Biochemie, Albert Ludwigs Universitat Freiburg, D 79104 Freiburg i Br, Germany
    Mol Genet Genomics 267:409-17. 2002
    ..The mode of regulation of P. aeruginosa hemA differs significantly from that described for the hemA gene of Escherichia coli K-12...
  90. ncbi Independent regulation of the divergent Escherichia coli nrfA and acsP1 promoters by a nucleoprotein assembly at a shared regulatory region
    Douglas F Browning
    School of Biosciences, The University of Birmingham, Birmingham B15 2TT, UK
    Mol Microbiol 43:687-701. 2002
    ..We conclude that the nrfA-acs intergenic region is folded into an ordered nucleoprotein structure that permits the two divergent promoters to be regulated independently in response to different physiological signals...
  91. pmc Structural analysis of conserved base pairs in protein-DNA complexes
    Leonid A Mirny
    Harvard MIT Division of Health Sciences and Technology, Room 16 343D, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA
    Nucleic Acids Res 30:1704-11. 2002
    ..Implications for prediction of the DNA-binding specificity are discussed...
  92. ncbi Suppression of FNR-dependent transcription activation at the Escherichia coli nir promoter by Fis, IHF and H-NS: modulation of transcription initiation by a complex nucleo-protein assembly
    D F Browning
    School of Biosciences, The University of Birmingham, Birmingham B15 2TT, UK
    Mol Microbiol 37:1258-69. 2000
    ..NarL and NarP can relieve both IHF- and Fis-mediated repression, but are unable to counteract H-NS-mediated repression...
  93. pmc Fis plays a role in Tn5 and IS50 transposition
    M D Weinreich
    Department of Biochemistry, University of Wisconsin Madison 53706
    J Bacteriol 174:4530-7. 1992
    ....
  94. ncbi The transcriptional activator protein FIS: DNA interactions and cooperative interactions with RNA polymerase at the Escherichia coli rrnB P1 promoter
    A J Bokal
    Department of Bacteriology, University of Wisconsin Madison 53706
    J Mol Biol 245:197-207. 1995
    ....
  95. pmc Identification of genes negatively regulated by Fis: Fis and RpoS comodulate growth-phase-dependent gene expression in Escherichia coli
    J Xu
    Department of Biological Chemistry, UCLA School of Medicine 90024
    J Bacteriol 177:938-47. 1995
    ..We conclude that Fis negatively regulates a diverse set of genes and that RpoS can function to both activate and inhibit the expression of specific genes...
  96. ncbi Expression of the gene encoding the major bacterial nucleotide protein H-NS is subject to transcriptional auto-repression
    M Falconi
    Department of Biology, University of Camerino, Italy
    Mol Microbiol 10:273-82. 1993
    ..Binding of H-NS to this site is probably the ultimate cause of transcriptional auto-repression...
  97. pmc Plasmids bearing hfq and the hns-like gene stpA complement hns mutants in modulating arginine decarboxylase gene expression in Escherichia coli
    X Shi
    Department of Biochemistry and Cell Biology, Rice University, Houston, Texas 77251
    J Bacteriol 176:6769-75. 1994
    ....
  98. pmc Regulation of aroL expression by TyrR protein and Trp repressor in Escherichia coli K-12
    B Lawley
    Department of Microbiology, University of Melbourne, Parkville, Victoria, Australia
    J Bacteriol 176:6921-30. 1994
    ..Identification of a potential integration host factor binding site and repression studies of a himA mutant support the notion that integration host factor binding normally exerts a negative effect on tyrosine-mediated repression...
  99. ncbi The dps promoter is activated by OxyR during growth and by IHF and sigma S in stationary phase
    S Altuvia
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892
    Mol Microbiol 13:265-72. 1994
    ..Our results suggest that the dps promoter is recognized by both sigma 70-holoenzyme and sigma S-holoenzyme, since OxyR acts through sigma 70 and the starts of the OxyR- and sigma S-dependent transcripts are identical...
  100. pmc Regulation of the Escherichia coli nrd operon: role of DNA supercoiling
    L Sun
    Department of Biochemistry, University of Minnesota, St Paul 55108
    J Bacteriol 176:4617-26. 1994
    ....
  101. pmc Escherichia coli Fis and DnaA proteins bind specifically to the nrd promoter region and affect expression of an nrd-lac fusion
    L B Augustin
    Department of Biochemistry, University of Minnesota, St Paul 55108
    J Bacteriol 176:378-87. 1994
    ..Assay of in vivo expression from wild-type and DnaA box-mutated nrd promoter fragments fused to lacZ on single-copy plasmids indicates a positive effect of DnaA binding on expression of nrd...

Research Grants73

  1. Host factors involved in viral RNA recombination
    Peter Nagy; Fiscal Year: 2006
    ..Knowledge of recombination promises untold health benefits by providing means to limit virus infections and to develop safer vaccines. ..
  2. Variation in M. tuberculosis in response to host selection
    Sarah Fortune; Fiscal Year: 2009
    ..In these studies, we expect to provide fundamental insights into the mechanisms and targets of diversifying immune selection in M. tuberculosis. ..
  3. Viral and Host Determinants of New World Alphaviral Entry
    ASIM AMINSHARIF AHMED; Fiscal Year: 2013
    ..Children's Hospital Boston is committed to this career development plan and has assured the candidate will be able to devote at least 85% full-time effort to the activities described in this proposal. ..
  4. Dependency of O-3 Induced Lung Mucus Hypersecretion on NQ01
    W Michael Foster; Fiscal Year: 2010
    ....
  5. Effect Ultraviolet Radiation on Latent M. tuberculosis Infection in Guinea Pigs
    Amminikutty Jeevan; Fiscal Year: 2010
    ..These studies will be addressed in the low-dose guinea pig model of pulmonary tuberculosis as the disease in these animals closely resembles the human disease. ..
  6. A novel allele influencing HIV infection among injection drug users
    Xiao Fang Yu; Fiscal Year: 2012
    ..abstract_text> ..
  7. Mechanisms of adaptive immunity to P. aeruginosa in the lung
    Gregory P Priebe; Fiscal Year: 2013
    ..Knowledge gained from these studies will support the development of better vaccines for pneumonia as well as an improved understanding of how to prevent lung infections in patients with cystic fibrosis. ..
  8. Control of alphavirous replacation in the nervous system
    Diane E Griffin; Fiscal Year: 2013
    ....
  9. Otitis media-associated pneumococcal genes
    Honggao Yan; Fiscal Year: 2013
    ..pneumoniae. The bacterial genes identified in this project can be targeted to treat and prevent S. pneumoniae disease by disarming the immune evasion arsenals of S. pneumoniae through novel anti-bacterial drugs and vaccines. ..
  10. Cellular Components Involved in Japanese Encephalitis Virus Entry
    Young Min Lee; Fiscal Year: 2013
    ....
  11. The Thrifty Microbiome: The Role of the Gut Microbiota in Obesity in the Amish
    Alan R Shuldiner; Fiscal Year: 2010
    ....
  12. Mechanisms of viral proteases in coronavirus replication and pathogenesis
    Andrew D Mesecar; Fiscal Year: 2013
    ..The results from this research will allow us to identify critical sites that mediate specificity of papain-like proteases and provide new insight into viral mechanisms for pathogenesis and evasion of the innate immune response. ..
  13. Murine coronavirus neurovirulence: role of type I interferon response
    Susan R Weiss; Fiscal Year: 2013
    ..Moreover, understanding type I IFN signaling in the CNS may aid in developing or refining therapeutic applications for type I IFNs, which are currently used in treatment of MS and hepatitis C. ..
  14. GAS Switch from Colonizing Bacterium to Invasive Pathogen
    Victor Nizet; Fiscal Year: 2012
    ..We are studying the ways in which the GAS bacteria shifts from an innocent member of our normal flora to an invasive pathogen, using molecular genetic techniques, assays of immune function, and mouse models of infection. ..
  15. Mechanism of murine coronavirus induced hepatitis
    Susan R Weiss; Fiscal Year: 2010
    ..Elucidation of the mechanism of MHV induced demyelination will contribute to the understanding of hepatitis in humans and in the long term contribute to the design of anti-viral therapies ..
  16. Host Factors in Fungal Allergy and Fibrosis
    Anuradha Ray; Fiscal Year: 2010
    ..The long-term objective of the SCCOR is to use the knowledge of the molecular pathways to improve diagnosis and treatment of patients suffering form chronic lung diseases particularly allergic and fibrotic lung diseases. ..
  17. HIV-1 regulation by MTb: pathogen and host factor
    Anne Goldfeld; Fiscal Year: 2010
    ..abstract_text> ..
  18. The role of the host Ca/Mn pump in emergence of novel viral RNA recombinants
    Peter Nagy; Fiscal Year: 2010
    ..The research holds promise of benefiting society by leading to groundbreaking results in the area of virus recombination/evolution, host-virus interaction and the emergence of new viruses. ..
  19. Pathophysiology of Adult T-cell Leukemia/Lymphoma
    UTPAL P DAVE; Fiscal Year: 2013
    ..Our aims are well-timed and can have a significant impact on a disease that is universally fatal upon diagnosis and advance our understanding of JAK3's role in T-cell neoplasia. ..
  20. Group II Intron Mobility and Gene Targeting
    Alan M Lambowitz; Fiscal Year: 2012
    ....
  21. Discovery of novel anti-HBV compounds targeting host factors
    Pavel A Petukhov; Fiscal Year: 2013
    ....
  22. Dental Caries: Whole Genome Association and Gene x Environment Studies
    Mary L Marazita; Fiscal Year: 2010
    ..Successful completion of this study will allow a better understanding of the biology of dental caries, and will lead to earlier identification of children at risk for dental caries, thereby allowing improved and targeted interventions. ..
  23. Protecutive immunity to human cholera in Bangladesh
    Edward T Ryan; Fiscal Year: 2013
    ..Identification of protective immunity against cholera could not only directly affect cholera vaccination strategies, but could be applicable to the development of improved vaccines against other mucosal pathogens. ..
  24. MOLECULAR MECHANISMS OF ALPHAVIRUS ENTRY AND EXIT
    Margaret Kielian; Fiscal Year: 2013
    ....
  25. Evaluation of the Effects of Chikungunya N-linked Glycosylation on Pathogenesis
    CHARLES EDWIN MCGEE; Fiscal Year: 2013
    ....
  26. IRON ACQUISITION IN BORDETELLA PERTUSSIS
    SANDRA KAY ARMSTRONG; Fiscal Year: 2013
    ..This increased understanding may lead to improved strategies to prevent growth of these, and other respiratory pathogens, in humans. ..
  27. Role of Macrophage Migration Inhibitory Factor (MIF) in Pneumococcal Pathogenesis
    Rituparna Das; Fiscal Year: 2013
    ....
  28. Mechanisms of pneumococcal persistence during carriage.
    JEFFREY NEAL WEISER; Fiscal Year: 2013
    ..Together these studies will provide mechanistic insight into the three main features of pneumococcal carriage;why it is common, why it is transient, and why it varies greatly among isolates by capsule type. ..
  29. Screens for host DNA replication and repair factors involved in viral infection
    Matthew D Weitzman; Fiscal Year: 2010
    ..These screens will identify cellular factors that regulate viral infection, will provide insights into virus-host interactions and fundamental cellular processes, and may suggest targets for anti-viral therapies. ..
  30. The Epidemiology of Home Allergens and Asthma
    Diane R Gold; Fiscal Year: 2010
    ..abstract_text> ..
  31. GFAP as a novel HIV/neuroAIDS biomarker
    Johnny J He; Fiscal Year: 2013
    ....
  32. Novel Inhibitors of Viral Replication
    Michael G Roth; Fiscal Year: 2013
    ..In sum, these studies will likely reveal novel leads for antiviral therapies as well as provide information on novel mechanisms of viral-host interactions and pathways. ..
  33. Benzophenones: Novel natural agents which inhibits HIV and Candida
    Ramiro Mendonca Murata; Fiscal Year: 2013
    ..The successful completion of the proposed studies has the potential to change the current strategies for dealing with both viral and microbial pandemics as well as promoting the value of therapeutic agents derived from natural products. ..
  34. Benzophenones: Novel natural agents which inhibits HIV and Candida
    Ramiro Mendonca Murata; Fiscal Year: 2011
    ..The successful completion of the proposed studies has the potential to change the current strategies for dealing with both viral and microbial pandemics as well as promoting the value of therapeutic agents derived from natural products. ..
  35. Assembly dynamics and role of PI4P enriched replication organelles for enterovira
    Ann Cali; Fiscal Year: 2013
    ..Our goals are to determine how these membrane properties regulate viral RNA replication and how specific cellular proteins are hijacked to impart these properties. ..
  36. Identifying Host-Factors Required for Toxin Entry and Toxicity
    ERIK HETT; Fiscal Year: 2010
    ..This work will illuminate the molecular mechanisms underlying resultant pathology and may provide clues for how to combat toxin-related diseases. ..
  37. Mechanistic Studies of the Function of Desmosterol Homeostasis in HCV Replication
    VALERIE ANNE AGUSTINO VILLAREAL; Fiscal Year: 2013
    ..Collectively, these experiments provide unique opportunities to study and validate desmosterol biosynthesis as a potential anti-HCV target and to explore a heretofore uncharacterized mechanism of regulation in cholesterol biosynthesis. ..
  38. Functional genomic analysis of host determinants of malaria infection
    Elizabeth Egan; Fiscal Year: 2013
    ..The results of this work will ultimately guide the rational development of host-targeted therapeutics for malaria. ..
  39. Critical Role of Nrf2 in Innate Immune Response and Survival During Sepsis
    Shyam Biswal; Fiscal Year: 2010
    ..and survival during sepsis. These studies may lead to the development of novel strategies based on Nrf2 for intervening in sepsis and improving survival. ..
  40. Host Factors for Hemorrhagic Fever
    JOHN NICHOLAS MISASI; Fiscal Year: 2013
    ..These determinants are a key component to understanding why these viruses cause hemorrhagic fever and may lead to new therapies and antivirals. ..
  41. Role of Immunogenetic Factors in Invasive Streptococcal Infections
    MALAK Y S KOTB; Fiscal Year: 2012
    ....
  42. REGULATION OF RETROTRANSPOSITION IN S. CEREVISIAE
    M Joan Curcio; Fiscal Year: 2013
    ..Cellular proteins that are required for retroviral replication are potential targets for the development of new drugs to treat AIDS. ..
  43. Mechanism of Pseudomonas-mediated epithelial cell damage
    Joanne N Engel; Fiscal Year: 2010
    ..It opens up the possibility of targeting host cell processes to treat infectious diseases. ..
  44. Genetic determinants of HSV-1 virulence in neurons
    Moriah L Szpara; Fiscal Year: 2013
    ..This research will investigate how HSV-1 virus isolated from one patient may differ from that of another. This will help us understand why some patients suffer more severe symptoms from HSV-1 infection than others. ..
  45. Non-invasive Fluorescent Imaging Mycobacterium tuberculosis Extrapulmonary Infect
    Ying Kong; Fiscal Year: 2013
    ..Identifying bacterial and host factors affecting EPTB would help in the development of improved diagnostic tools, anti-tuberculosis therapies and vaccines. ..
  46. Mechanisms of the M protein in group A Streptococcus virulence
    Victor Nizet; Fiscal Year: 2013
    ..Ghosh will directly supervise three postdoctoral researchers focusing on biochemical and structural approaches, and Dr. Nizet will directly supervise one postdoctoral researcher focusing on microbiological and immunological approaches. ..
  47. Neurovirulence of murine coronavirus: roles of viral genes
    Susan R Weiss; Fiscal Year: 2010
    ..We believe these studies will lead to a better understanding of the determinants of neurovirulence during MHV infection. ..
  48. Dengue Virus Genome Encapsidation and Its Interplay with Host Lipid Droplets
    Andrea Gamarnik; Fiscal Year: 2013
    ..Importantly, the studies proposed will provide new information about a viral process that has been unexplored for antiviral intervention. ..
  49. Inhibition of Toxin Translocation Can Reverse Cholera and ETEC-Mediated Diarrhea
    KENNETH R TETER; Fiscal Year: 2013
    ..Here, we will establish these parameters and demonstrate recovery from intoxication is possible with treatments that lower the levels of intracellular toxin. ..
  50. HSV latency and reactivation and the novel neuronal regulation of VP16 in vivo.
    Nancy M Sawtell; Fiscal Year: 2013
    ..The outcomes of this work will identify transcription factors or protein modifying enzymes that could be targets for future development of therapeutic interventions for HSV reactivation. ..
  51. Mechanism and Therapy of HIV Encephalitis
    Shilpa J Buch; Fiscal Year: 2012
    ....
  52. NEONATAL CANDIDIASIS &IMMUNE COMPROMISE DURING DEVELOPMENT
    JOSEPH MARK BLISS; Fiscal Year: 2011
    ..abstract_text> ..
  53. HIV Nucleoside Analogs Translational Studies: Resistance and Metabolism
    Elijah Paintsil; Fiscal Year: 2012
    ....
  54. Live Attenuated Recombinant Bacterial Delivery of Polysaccharide Vaccine Antigens
    Joanna B Goldberg; Fiscal Year: 2010
    ..We anticipate that this knowledge will also be applicable to other pathogens. ..
  55. Analysis of novel in vivo derived Plasmodium falciparum transcriptional profiles
    JOHANNA PATRICIA DAILY; Fiscal Year: 2012
    ..abstract_text> ..
  56. Nipsnap1: A Novel Negative Regulator of Hepatitis C Virus Replication
    BRIANA M WEISER; Fiscal Year: 2013
    ..Through immunoprecipitation coupled mass spectrometry, we hope to generate a body of data to devise a model by which hepatitis C viral proteins utilize different host factors to manipulate membranes for replication and assembly. ..
  57. Role of Lipids in Hepatitis C Virus Maturation
    Aleem Siddiqui; Fiscal Year: 2013
    ..These studies will reveal unique insights into the mechanisms of HCV maturation, secretion and budding processes and provide a unique model for other RNA viruses. ..
  58. Domoic Acid Neurotoxicity in Native Americans
    Lynn M Grattan; Fiscal Year: 2013
    ..The diagnosis and prognosis for ASP will be defined and established safety levels for exposure will be re-evaluated to insure they are protective of persons with long term, repeated exposure to DA. ..
  59. Role of Host miRNAs in Persistent HIV Macrophage Infection
    Judy Lieberman; Fiscal Year: 2011
    ....
  60. Interactions of Ebola virus glycoproteins with host cells
    Paul Bates; Fiscal Year: 2013
    ....
  61. Quantifying Drug Adherence and Drug Exposure to Antiretroviral Therapy
    JOSE RAMON CASTILLO-MANCILLA; Fiscal Year: 2013
    ..The proposed research will develop a new pharmacologic approach to quantify drug exposure and adherence, which could lead to more efficacious HIV treatment and prevention strategies. ..
  62. Apolipoprotein B and Control of S. aureus Quorum Sensing
    PAMELA RANEL HALL; Fiscal Year: 2013
    ..abstract_text> ..
  63. Cutaneous Leishmaniasis in West Africa: the Parasite, Vector and Disease
    Seydou Doumbia; Fiscal Year: 2013
    ..Understanding of these relationship is important for vaccine development and field trials of control strategies. ..
  64. Functional role of a host metabolic enzyme in viral replication
    Peter Nagy; Fiscal Year: 2010
    ..The proposed work will lead to deeper understanding of the role of the host in RNA virus infections and likely open new antiviral strategies. ..
  65. Cohort Study of Genetic Susceptibility to Cutaneous Malignant Melanoma
    Jiali Han; Fiscal Year: 2010
    ..This research will contribute to the scientific basis for identifying individuals at high risk for melanoma and providing individualized risk management strategies. ..
  66. Foregut microbiome in development of esophageal adenocarcinoma
    Zhiheng Pei; Fiscal Year: 2009
    ..If GERD represents a microbiome-related disease, it could be possible to design new antibiotic or probiotic treatment strategies to prevent GERD and reverse the current trend of increasing rate of esophageal adenocarcinoma. ..
  67. Host factors in the alphavirus replication complex
    Margaret MacDonald; Fiscal Year: 2009
    ..Understanding the components of and molecular interactions within the Alphavirus minus and plus strand replicases will facilitate future exploration for inhibitors of these important pathogens. ..
  68. Development of a genetically tractable nematode model of alphavirus infection
    Margaret R Macdonald; Fiscal Year: 2010
    ..Understanding the virus-host interaction in greater detail may lead to the development of specific therapies. ..
  69. Risk factors for gastric disease in pediatric H. pylori
    Benjamin Gold; Fiscal Year: 2006
    ..We propose to further our previous work with critically lacking studies from a multivariate approach leading to a better understanding of the gastroduodenal disease sequelae and overall pathobiology of Hp infection in humans. ..
  70. EFFECTORS AND IINHIBITORS OF SARS VIRUS POLYMERASE
    Neerja Kaushik Basu; Fiscal Year: 2006
    ..Further, analysis of the sensitivity of the SARS-CoV RdRp towards a panel of known and novel inhibitors of polymerases will provide new leads in the quest for specific antiviral agents against the SARS-CoV RdRp. ..
  71. Modulation of c-MYC Functions in an HTLV-1 Model for Carcinogenesis
    Robert Harrod; Fiscal Year: 2009
    ..These studies may lead to the development of targeted therapeutic strategies to inhibit c-MYC oncogenic functions in hematological malignancies and solid tumors. ..
  72. Bacterial biofilm-host interaction during Vibrio cholerae infection
    Jun Zhu; Fiscal Year: 2009
    ..cholerae's genetic controls in host-pathogen interactions, with the goal of better understanding V. cholerae pathogenesis and, potentially, discovering novel treatment options for the cholera disease. ..
  73. Developing a yeast system to study virus-host interactions in Mononegavirales
    VALERY GRDZELISHVILI; Fiscal Year: 2009
    ....