flagellin

Summary

Summary: A protein with a molecular weight of 40,000 isolated from bacterial flagella. At appropriate pH and salt concentration, three flagellin monomers can spontaneously reaggregate to form structures which appear identical to intact flagella.

Top Publications

  1. ncbi The innate immune response to bacterial flagellin is mediated by Toll-like receptor 5
    F Hayashi
    Department of Immunology, University of Washington, Seattle 98195, USA
    Nature 410:1099-103. 2001
  2. ncbi FLS2: an LRR receptor-like kinase involved in the perception of the bacterial elicitor flagellin in Arabidopsis
    L Gomez-Gomez
    Friedrich Miescher Institut, Basel, Switzerland
    Mol Cell 5:1003-11. 2000
  3. ncbi Bacterial disease resistance in Arabidopsis through flagellin perception
    Cyril Zipfel
    Friedrich Miescher Institute for Biomedical Research, PO Box 2543, CH 4002 Basel, Switzerland
    Nature 428:764-7. 2004
  4. ncbi Perception of the bacterial PAMP EF-Tu by the receptor EFR restricts Agrobacterium-mediated transformation
    Cyril Zipfel
    Botanical Institute, Zurich Basel Plant Science Centre, University of Basel, Hebelstrasse 1, CH 4056 Basel, Switzerland
    Cell 125:749-60. 2006
  5. ncbi Plants have a sensitive perception system for the most conserved domain of bacterial flagellin
    G Felix
    Friedrich Miescher Institute, Basel, Switzerland
    Plant J 18:265-76. 1999
  6. ncbi The NLRC4 inflammasome receptors for bacterial flagellin and type III secretion apparatus
    Yue Zhao
    Graduate Program in Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, China
    Nature 477:596-600. 2011
  7. ncbi Coordinating assembly of a bacterial macromolecular machine
    Fabienne F V Chevance
    Department of Biology, University of Utah, 257 South 1400 East, Salt Lake City, Utah 84112, USA
    Nat Rev Microbiol 6:455-65. 2008
  8. pmc Differential innate immune signalling via Ca(2+) sensor protein kinases
    Marie Boudsocq
    Department of Genetics, Harvard Medical School, Center for Computational and Integrative Biology, Massachusetts General Hospital, Massachusetts 02114, USA
    Nature 464:418-22. 2010
  9. ncbi A flagellin-induced complex of the receptor FLS2 and BAK1 initiates plant defence
    Delphine Chinchilla
    Zurich Basel Plant Science Center, Botanical Institute, University of Basel, Hebelstrasse 1, 4056 Basel, Switzerland
    Nature 448:497-500. 2007
  10. ncbi Plant stomata function in innate immunity against bacterial invasion
    Maeli Melotto
    Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, MI 48824, USA
    Cell 126:969-80. 2006

Detail Information

Publications303 found, 100 shown here

  1. ncbi The innate immune response to bacterial flagellin is mediated by Toll-like receptor 5
    F Hayashi
    Department of Immunology, University of Washington, Seattle 98195, USA
    Nature 410:1099-103. 2001
    ..b>Flagellin, a principal component of bacterial flagella, is a virulence factor that is recognized by the innate immune ..
  2. ncbi FLS2: an LRR receptor-like kinase involved in the perception of the bacterial elicitor flagellin in Arabidopsis
    L Gomez-Gomez
    Friedrich Miescher Institut, Basel, Switzerland
    Mol Cell 5:1003-11. 2000
    b>Flagellin, the main protein of the bacterial flagella, elicits defence responses and alters growth in Arabidopsis seedlings. Previously, we identified the FLS1 locus, which confers flagellin insensitivity in Ws-0...
  3. ncbi Bacterial disease resistance in Arabidopsis through flagellin perception
    Cyril Zipfel
    Friedrich Miescher Institute for Biomedical Research, PO Box 2543, CH 4002 Basel, Switzerland
    Nature 428:764-7. 2004
    ..and animals recognize microbial invaders by detecting pathogen-associated molecular patterns (PAMPs) such as flagellin. However, the importance of flagellin perception for disease resistance has, until now, not been demonstrated...
  4. ncbi Perception of the bacterial PAMP EF-Tu by the receptor EFR restricts Agrobacterium-mediated transformation
    Cyril Zipfel
    Botanical Institute, Zurich Basel Plant Science Centre, University of Basel, Hebelstrasse 1, CH 4056 Basel, Switzerland
    Cell 125:749-60. 2006
    ..Arabidopsis plants detect a variety of PAMPs including conserved domains of bacterial flagellin and of bacterial EF-Tu...
  5. ncbi Plants have a sensitive perception system for the most conserved domain of bacterial flagellin
    G Felix
    Friedrich Miescher Institute, Basel, Switzerland
    Plant J 18:265-76. 1999
    ..These antagonists suppressed the plant's response to flagellin, crude bacterial extracts and living bacterial cells...
  6. ncbi The NLRC4 inflammasome receptors for bacterial flagellin and type III secretion apparatus
    Yue Zhao
    Graduate Program in Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, China
    Nature 477:596-600. 2011
    ..The inflammasome assembled by the NOD-like receptor (NLR) protein NLRC4 responds to bacterial flagellin and a conserved type III secretion system (TTSS) rod component...
  7. ncbi Coordinating assembly of a bacterial macromolecular machine
    Fabienne F V Chevance
    Department of Biology, University of Utah, 257 South 1400 East, Salt Lake City, Utah 84112, USA
    Nat Rev Microbiol 6:455-65. 2008
    ..In this Review, we discuss this mechanism, together with others that also coordinate gene regulation and flagellar assembly in Gram-negative bacteria...
  8. pmc Differential innate immune signalling via Ca(2+) sensor protein kinases
    Marie Boudsocq
    Department of Genetics, Harvard Medical School, Center for Computational and Integrative Biology, Massachusetts General Hospital, Massachusetts 02114, USA
    Nature 464:418-22. 2010
    ..recognition of pathogen- or microbe-associated molecular patterns (PAMPs or MAMPs, respectively), such as flagellin, initiates convergent signalling pathways involving mitogen-activated protein kinase (MAPK) cascades and global ..
  9. ncbi A flagellin-induced complex of the receptor FLS2 and BAK1 initiates plant defence
    Delphine Chinchilla
    Zurich Basel Plant Science Center, Botanical Institute, University of Basel, Hebelstrasse 1, 4056 Basel, Switzerland
    Nature 448:497-500. 2007
    ..In Arabidopsis thaliana, the leucine-rich repeat receptor kinases flagellin-sensitive 2 (FLS2) (ref. 2) and elongation factor Tu receptor (EFR) (ref...
  10. ncbi Plant stomata function in innate immunity against bacterial invasion
    Maeli Melotto
    Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, MI 48824, USA
    Cell 126:969-80. 2006
    ..We provide evidence that supports a model in which stomata, as part of an integral innate immune system, act as a barrier against bacterial infection...
  11. pmc Interleukin 23 production by intestinal CD103(+)CD11b(+) dendritic cells in response to bacterial flagellin enhances mucosal innate immune defense
    Melissa A Kinnebrew
    Infectious Diseases Service, Department of Medicine, Immunology Program, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA
    Immunity 36:276-87. 2012
    ..Systemic administration of flagellin, a bacterial protein that stimulates Toll-like receptor 5 (TLR5), induces epithelial expression of RegIIIγ and ..
  12. ncbi An agonist of toll-like receptor 5 has radioprotective activity in mouse and primate models
    Lyudmila G Burdelya
    Department of Cell Stress Biology, Roswell Park Cancer Institute, Buffalo, NY 14263, USA
    Science 320:226-30. 2008
    ..We studied CBLB502, a polypeptide drug derived from Salmonella flagellin that binds to Toll-like receptor 5 (TLR5) and activates nuclear factor-kappaB signaling...
  13. pmc Evasion of Toll-like receptor 5 by flagellated bacteria
    Erica Andersen-Nissen
    Institute for Systems Biology, 1441 North 34th Street, Seattle, WA 98103, USA
    Proc Natl Acad Sci U S A 102:9247-52. 2005
    Toll-like receptor 5 (TLR5) recognizes an evolutionarily conserved site on bacterial flagellin that is required for flagellar filament assembly and motility...
  14. pmc The Arabidopsis receptor kinase FLS2 binds flg22 and determines the specificity of flagellin perception
    Delphine Chinchilla
    Zurich Basel Plant Science Center, Botanisches Institut der Universität Basel, CH 4056 Basel, Switzerland
    Plant Cell 18:465-76. 2006
    b>Flagellin, the main building block of the bacterial flagellum, acts as a pathogen-associated molecular pattern triggering the innate immune response in animals and plants...
  15. pmc The receptor-like kinase SERK3/BAK1 is a central regulator of innate immunity in plants
    Antje Heese
    Department of Biochemistry, University of Missouri, 540G Bond Life Sciences Center, 1201 Rollins Road, Columbia, MO 65211, USA
    Proc Natl Acad Sci U S A 104:12217-22. 2007
    ..In Arabidopsis thaliana, AtSERK3/BAK1 rapidly enters an elicitor-dependent complex with FLAGELLIN SENSING 2 (FLS2), the receptor for the bacterial PAMP flagellin and its peptide derivative flg22...
  16. pmc Structural basis of TLR5-flagellin recognition and signaling
    Sung Il Yoon
    Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Science 335:859-64. 2012
    Toll-like receptor 5 (TLR5) binding to bacterial flagellin activates signaling through the transcription factor NF-κB and triggers an innate immune response to the invading pathogen...
  17. ncbi Cytoplasmic flagellin activates caspase-1 and secretion of interleukin 1beta via Ipaf
    Edward A Miao
    Institute for Systems Biology, Seattle, Washington 98103, USA
    Nat Immunol 7:569-75. 2006
    ..We show here that cytosolic bacterial flagellin activated caspase-1 through Ipaf but was independent of Toll-like receptor 5, a known flagellin sensor...
  18. pmc Flagellin-deficient Legionella mutants evade caspase-1- and Naip5-mediated macrophage immunity
    Tao Ren
    Genetics Department, Harvard Medical School, Boston, Massachusetts, USA
    PLoS Pathog 2:e18. 2006
    ..pneumophila that evade macrophage innate immunity. All mutants were deficient in expression of flagellin, the primary flagellar subunit, and failed to induce caspase-1-mediated macrophage death...
  19. pmc Caspase-11 stimulates rapid flagellin-independent pyroptosis in response to Legionella pneumophila
    Christopher L Case
    Department of Microbial Pathogenesis, Yale University School of Medicine, New Haven, CT 06536, USA
    Proc Natl Acad Sci U S A 110:1851-6. 2013
    A flagellin-independent caspase-1 activation pathway that does not require NAIP5 or NRLC4 is induced by the intracellular pathogen Legionella pneumophila. Here we demonstrate that this pathway requires caspase-11...
  20. ncbi Cytosolic flagellin requires Ipaf for activation of caspase-1 and interleukin 1beta in salmonella-infected macrophages
    Luigi Franchi
    Department of Pathology and Comprehensive Cancer Center, University of Michigan Medical School, Ann Arbor, Michigan 48109, USA
    Nat Immunol 7:576-82. 2006
    ..Here we demonstrate that salmonella strains either lacking flagellin or expressing mutant flagellin were deficient in activation of caspase-1 and in interleukin 1beta secretion, ..
  21. ncbi Toll-like receptor 5 recognizes a conserved site on flagellin required for protofilament formation and bacterial motility
    Kelly D Smith
    Institute for Systems Biology, 1441 North 34th Street, Seattle, Washington 98103, USA
    Nat Immunol 4:1247-53. 2003
    Toll-like receptor 5 (TLR5) recognizes bacterial flagellin and activates host inflammatory responses. In this study, we examine the nature of the TLR5-flagellin interaction...
  22. ncbi A single locus determines sensitivity to bacterial flagellin in Arabidopsis thaliana
    L Gomez-Gomez
    Friedrich Miescher Institute, Basel, Switzerland
    Plant J 18:277-84. 1999
    Peptides corresponding to the most conserved domain of eubacterial flagellin act as potent elicitors in cells of different plant species...
  23. pmc Critical function for Naip5 in inflammasome activation by a conserved carboxy-terminal domain of flagellin
    Karla L Lightfield
    School of Public Health, University of California, Berkeley, CA 94720, USA
    Nat Immunol 9:1171-8. 2008
    ..Here we demonstrate that 35 amino acids of the carboxyl terminus of flagellin triggered inflammasome activation in the absence of bacterial contaminants or secretion systems...
  24. ncbi Regulation of Legionella phagosome maturation and infection through flagellin and host Ipaf
    Amal Amer
    Department of Pathology and Comprehensive Cancer Center, University of Michigan Medical School, Ann Arbor, Michigan 48109, USA
    J Biol Chem 281:35217-23. 2006
    ..Here we demonstrate that the presence of flagellin and a competent type IV secretion system are critical for Legionella to activate caspase-1 in macrophages...
  25. pmc Cytosolic recognition of flagellin by mouse macrophages restricts Legionella pneumophila infection
    Ari B Molofsky
    Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI 48109, USA
    J Exp Med 203:1093-104. 2006
    ..pneumophila replication and initiated a proinflammatory program of cell death when flagellin contaminated their cytosol...
  26. ncbi Potent immunogenicity and efficacy of a universal influenza vaccine candidate comprising a recombinant fusion protein linking influenza M2e to the TLR5 ligand flagellin
    James W Huleatt
    VaxInnate Corporation, 300 George Street, New Haven, CT 06511, USA
    Vaccine 26:201-14. 2008
    ..A recombinant protein comprising the TLR5 ligand flagellin fused to four tandem copies of the ectodomain of the conserved influenza matrix protein M2 (M2e) was expressed ..
  27. pmc Flagellin-F1-V fusion protein is an effective plague vaccine in mice and two species of nonhuman primates
    Steven B Mizel
    Department of Microbiology and Immunology, Wake Forest University School of Medicine, Medical Center Blvd, Winston Salem, NC 27157 1064, USA
    Clin Vaccine Immunol 16:21-8. 2009
    A number of studies have clearly demonstrated that flagellin is a potent adjuvant that promotes robust immune responses when it is given with a protein antigen...
  28. ncbi Flagellin promotes the proliferation of gastric cancer cells via the Toll-like receptor 5
    Eun Jung Song
    Department of Biochemistry, College of Medicine, Konyang University, Daejeon 302 711, Republic of Korea
    Int J Mol Med 28:115-9. 2011
    ..The TLR5 agonist, flagellin, induced IL-8 production and NF-κB activation in the gastric cancer cell lines...
  29. pmc A receptor-like cytoplasmic kinase, BIK1, associates with a flagellin receptor complex to initiate plant innate immunity
    Dongping Lu
    Department of Biochemistry and Biophysics, Institute for Plant Genomics and Biotechnology, Texas A and M University, College Station, TX 77843, USA
    Proc Natl Acad Sci U S A 107:496-501. 2010
    ..The plant PRR FLS2, a leucine-rich repeat-receptor kinase, recognizes bacterial flagellin and initiates immune signaling by association with another leucine-rich repeat-receptor-like kinase, BAK1...
  30. pmc Arabidopsis MPK3 and MPK6 play different roles in basal and oligogalacturonide- or flagellin-induced resistance against Botrytis cinerea
    Roberta Galletti
    Istituto Pasteur Fondazione Cenci Bolognetti, Dipartimento di Biologia e Biotecnologie Charles Darwin, Sapienza Universita di Roma, 00185 Rome, Italy
    Plant Physiol 157:804-14. 2011
    ..These data indicate that OG- and flg22-induced defense responses effective against B. cinerea are mainly dependent on MAPKs, with a greater contribution of MPK6...
  31. pmc A prominent role of the flagellin receptor FLAGELLIN-SENSING2 in mediating stomatal response to Pseudomonas syringae pv tomato DC3000 in Arabidopsis
    Weiqing Zeng
    Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824 1312
    Plant Physiol 153:1188-98. 2010
    The FLAGELLIN-SENSING2 (FLS2) receptor kinase recognizes bacterial flagellin and initiates a battery of downstream defense responses to reduce bacterial invasion through stomata in the epidermis and bacterial multiplication in the ..
  32. pmc The induction of colitis and ileitis in mice is associated with marked increases in intestinal concentrations of stimulants of TLRs 2, 4, and 5
    Clett Erridge
    Department of Cardiovascular Sciences, University of Leicester, Leicester, United Kingdom
    PLoS ONE 5:e9125. 2010
    ..We aimed to establish if intestinal concentrations of proinflammatory bacterial ligands of TLR2, TLR4, or TLR5 may be altered in murine IBD models, and to characterize which of the major bacterial groups may contribute to each signal...
  33. pmc Flagellin as an adjuvant: cellular mechanisms and potential
    Steven B Mizel
    Department of Microbiology and Immunology, Wake Forest University School of Medicine, Winston Salem, NC 27157, USA
    J Immunol 185:5677-82. 2010
    b>Flagellin is a potent activator of a broad range of cell types involved in innate and adaptive immunity...
  34. pmc Mutagenesis of Burkholderia pseudomallei with Tn5-OT182: isolation of motility mutants and molecular characterization of the flagellin structural gene
    D DeShazer
    Department of Microbiology and Infectious Diseases, University of Calgary Health Sciences Centre, Alberta, Canada
    J Bacteriol 179:2116-25. 1997
    ..We used Tn5-OT182 to identify the flagellin structural gene, fliC. We screened 3,500 transposon mutants and identified 28 motility mutants...
  35. pmc Pseudomonas evades immune recognition of flagellin in both mammals and plants
    Bart W Bardoel
    Medical Microbiology, University Medical Center Utrecht, Utrecht, The Netherlands
    PLoS Pathog 7:e1002206. 2011
    The building blocks of bacterial flagella, flagellin monomers, are potent stimulators of host innate immune systems...
  36. ncbi Activation of the Arabidopsis thaliana mitogen-activated protein kinase MPK11 by the flagellin-derived elicitor peptide, flg22
    Gerit Bethke
    Leibniz Institute of Plant Biochemistry, Stress and Developmental Biology, Weinberg 3, D 06120 Halle, Germany
    Mol Plant Microbe Interact 25:471-80. 2012
    ..infection or treatments with conserved pathogen-associated molecular patterns (PAMPs) such as the bacterial flagellin-derived flg22 peptide are known to activate three Arabidopsis thaliana MAPK: MPK3, MPK4, and MPK6...
  37. ncbi Early events in the pathogenicity of Pseudomonas syringae on Nicotiana benthamiana
    Dagmar R Hann
    The Sainsbury Laboratory, John Innes Centre, Colney Lane, Norwich NR4 7UH, UK
    Plant J 49:607-18. 2007
    ..One important PAMP is the flagellin protein derived from motile bacteria...
  38. pmc The transcriptional innate immune response to flg22. Interplay and overlap with Avr gene-dependent defense responses and bacterial pathogenesis
    Lionel Navarro
    The Sainsbury Laboratory, John Innes Centre, Norwich Research Park, United Kingdom
    Plant Physiol 135:1113-28. 2004
    Animals and plants carry recognition systems to sense bacterial flagellin. Flagellin perception in Arabidopsis involves FLS2, a Leu-rich-repeat receptor kinase...
  39. ncbi In vivo, fliC expression by Salmonella enterica serovar Typhimurium is heterogeneous, regulated by ClpX, and anatomically restricted
    Lisa A Cummings
    Department of Laboratory Medicine, University of Washington, Seattle, WA 98195, USA
    Mol Microbiol 61:795-809. 2006
    ....
  40. pmc Identification and mutational analysis of Arabidopsis FLS2 leucine-rich repeat domain residues that contribute to flagellin perception
    F Mark Dunning
    Department of Plant Pathology, University of Wisconsin, Madison, Wisconsin 53706
    Plant Cell 19:3297-313. 2007
    ..to study leucine-rich repeat (LRR) domains and were used to identify residues within the Arabidopsis thaliana FLAGELLIN-SENSING2 (FLS2) LRR that contribute to flagellin perception...
  41. ncbi Toll-like receptor (TLR) 2 and TLR5, but not TLR4, are required for Helicobacter pylori-induced NF-kappa B activation and chemokine expression by epithelial cells
    Michael F Smith
    University of Virginia Health System, Digestive Health Center of Excellence and Department of Microbiology, Charlottesville, Virginia 22908 0708, USA
    J Biol Chem 278:32552-60. 2003
    ..pylori strain 26695 activated NF-kappa B in HEK293 via TLR2 but not TLR4. Partially purified flagellin from H. pylori was also capable of inducing NF-kappa B activation in HEK cells transfected with TLR5...
  42. pmc Immune recognition of Pseudomonas aeruginosa mediated by the IPAF/NLRC4 inflammasome
    Fayyaz S Sutterwala
    Department of Immunobiology, Yale University School of Medicine, New Haven, CT 06520, USA
    J Exp Med 204:3235-45. 2007
    ..aeruginosa results in activation of caspase-1 in an IPAF-dependent, but flagellin-independent, manner. Macrophages deficient in IPAF or caspase-1 were markedly resistant to P...
  43. pmc CsrA-FliW interaction governs flagellin homeostasis and a checkpoint on flagellar morphogenesis in Bacillus subtilis
    Sampriti Mukherjee
    Department of Biology, Indiana University, Bloomington, IN 47408, USA
    Mol Microbiol 82:447-61. 2011
    ..FliW, the first protein antagonist of CsrA activity that constitutes a partner switching mechanism to control flagellin synthesis in the Gram-positive organism Bacillus subtilis...
  44. ncbi Antitumor activity of the TLR-5 ligand flagellin in mouse models of cancer
    Lucia Sfondrini
    Institute of Pathology, University of Milan, Milan, Italy
    J Immunol 176:6624-30. 2006
    b>Flagellin, the structural protein subunit of the bacterial flagellum, is specifically recognized by TLR-5 and has potent immunomodulatory effects...
  45. pmc A crucial role of Flagellin in the induction of airway mucus production by Pseudomonas aeruginosa
    Fatima Ben Mohamed
    Unité de Défense Innée et Inflammation, Institut Pasteur, Paris, France
    PLoS ONE 7:e39888. 2012
    Pseudomonas aeruginosa is an opportunistic pathogen involved in nosocomial infections. Flagellin is a P. aeruginosa virulence factor involved in host response to this pathogen. We examined the role of flagellin in P...
  46. pmc Changes in flagellin glycosylation affect Campylobacter autoagglutination and virulence
    Patricia Guerry
    Enteric Diseases Department, Naval Medical Research Center, 503 Robert Grant Ave, Silver Spring, MD, USA
    Mol Microbiol 60:299-311. 2006
    Analysis of the complete flagellin glycosylation locus of Campylobacter jejuni strain 81-176 revealed a less complex genomic organization than the corresponding region in the genome strain, C. jejuni NCTC 11168...
  47. pmc The Arabidopsis flagellin receptor FLS2 mediates the perception of Xanthomonas Ax21 secreted peptides
    Cristian H Danna
    Department of Genetics, Harvard Medical School, Boston, MA 02114, USA
    Proc Natl Acad Sci U S A 108:9286-91. 2011
    ..The Arabidopsis thaliana receptor-like kinase FLS2 is the pattern-recognition receptor for bacterial flagellin. Similarly to FLS2, the rice transmembrane protein XA21 is the receptor for the sulfated form of the Xanthomonas ..
  48. pmc Within-species flagellin polymorphism in Xanthomonas campestris pv campestris and its impact on elicitation of Arabidopsis FLAGELLIN SENSING2-dependent defenses
    Wenxian Sun
    Department of Plant Pathology, University of Wisconsin, Madison, Wisconsin 53705, USA
    Plant Cell 18:764-79. 2006
    ..Arabidopsis thaliana FLAGELLIN SENSING2 (FLS2), a transmembrane leucine-rich repeat kinase, confers flagellin responsiveness...
  49. ncbi Induction of a potent immune response in the elderly using the TLR-5 agonist, flagellin, with a recombinant hemagglutinin influenza-flagellin fusion vaccine (VAX125, STF2.HA1 SI)
    David N Taylor
    VaxInnate Corporation, 3 Cedar Brook Drive, Cranbury, NJ 08512, USA
    Vaccine 29:4897-902. 2011
    ..We evaluated a novel influenza vaccine consisting of the globular head of the HA1 domain of the A/Solomon Islands/3/2006 (H1N1) influenza virus (VAX125) genetically fused to the TLR5 ligand, flagellin, and produced in Escherichia coli.
  50. ncbi Safety and immunogenicity of a recombinant M2e-flagellin influenza vaccine (STF2.4xM2e) in healthy adults
    Christine B Turley
    Sealy Center for Vaccine Development, University of Texas Medical Branch Galveston, 301 University Boulevard, Galveston, TX 77555 0351, USA
    Vaccine 29:5145-52. 2011
    ..is a recombinant fusion protein that links four tandem copies of the M2e antigen to Salmonella typhimurium flagellin, a TLR5 ligand used as an adjuvant...
  51. pmc Genetic analysis of spirochete flagellin proteins and their involvement in motility, filament assembly, and flagellar morphology
    Chunhao Li
    Department of Oral Biology, State University of New York at Buffalo, Buffalo, New York 14214, USA
    J Bacteriol 190:5607-15. 2008
    ..Finally, linear elasticity theory indicates that flagellar stiffness directly affects the spirochete's swimming speed...
  52. ncbi Flagellin induces myeloid-derived suppressor cells: implications for Pseudomonas aeruginosa infection in cystic fibrosis lung disease
    Nikolaus Rieber
    Department of Pediatrics I, University of Tubingen, Tubingen, 72076 Germany
    J Immunol 190:1276-84. 2013
    ..activates the innate immune system, mainly mediated through pathogen-associated molecular patterns, such as flagellin. However, the host is unable to eradicate this flagellated bacterium efficiently...
  53. pmc Deletions in the repertoire of Pseudomonas syringae pv. tomato DC3000 type III secretion effector genes reveal functional overlap among effectors
    Brian H Kvitko
    Department of Plant Pathology and Plant Microbe Biology, Cornell University, Ithaca, New York, USA
    PLoS Pathog 5:e1000388. 2009
    ....
  54. pmc Direct stimulation of tlr5+/+ CD11c+ cells is necessary for the adjuvant activity of flagellin
    John T Bates
    Department of Microbiology and Immunology, Wake Forest University School of Medicine, Winston Salem, NC 27157, USA
    J Immunol 182:7539-47. 2009
    b>Flagellin is a highly effective adjuvant, but the cellular mechanism underlying this activity remains uncertain. More specifically, no consensus exists as to whether flagellin activates dendritic cells (DC) directly or indirectly...
  55. pmc Interleukin-8 production by human airway epithelial cells in response to Pseudomonas aeruginosa clinical isolates expressing type a or type b flagellins
    Kathleen K Shanks
    Division of Pulmonology Allergy, Department of Pediatrics, University of Maryland School of Medicine, 655 W Baltimore Street, Baltimore, MD 21201, USA
    Clin Vaccine Immunol 17:1196-202. 2010
    Pseudomonas aeruginosa lung infection is a major cause of morbidity and mortality worldwide. P. aeruginosa flagellin, the main structural protein of the flagellar filament, is a virulence factor with proinflammatory activity on ..
  56. ncbi Flagellin contamination of recombinant heat shock protein 70 is responsible for its activity on T cells
    Zhiyong Ye
    Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, Block MD7, 05 10, 8 Medical Drive, Singapore 117597, Singapore
    J Biol Chem 282:4479-84. 2007
    ..However, recombinant Hsp70 expressed in Escherichia coli DeltafliC strain completely lost its ability to costimulate T cells. Thus, the activation of T cells by recombinant Hsp70 is ascribed to flagellin contamination.
  57. ncbi Dominant-negative TLR5 polymorphism reduces adaptive immune response to flagellin and negatively associates with Crohn's disease
    Andrew T Gewirtz
    Department of Pathology and Laboratory Medicine, Epithelial Pathobiology Unit, Emory University School of Medicine, Atlanta, GA 30322, USA
    Am J Physiol Gastrointest Liver Physiol 290:G1157-63. 2006
    Crohn's disease (CD) is associated with elevated adaptive immunity to commensal microbes, with flagellin being a dominant antigen...
  58. pmc Salmonella enterica serovar enteritidis core O polysaccharide conjugated to H:g,m flagellin as a candidate vaccine for protection against invasive infection with S. enteritidis
    Raphael Simon
    Center for Vaccine Development, University of Maryland, Baltimore, Baltimore, MD 21201, USA
    Infect Immun 79:4240-9. 2011
    ..We conjugated core polysaccharide-OPS (COPS) of Salmonella Enteritidis lipopolysaccharide (LPS) to flagellin protein from the homologous strain...
  59. pmc Functional characterization of flagellin glycosylation in Campylobacter jejuni 81-176
    Cheryl P Ewing
    Enteric Diseases Department, Naval Medical Research Center, 503 Robert Grant Ave, Silver Spring, MD 20910, USA
    J Bacteriol 191:7086-93. 2009
    The major flagellin of Campylobacter jejuni strain 81-176, FlaA, has been shown to be glycosylated at 19 serine or threonine sites, and this glycosylation is required for flagellar filament formation...
  60. pmc Pseudomonas aeruginosa activates caspase 1 through Ipaf
    Edward A Miao
    Institute for Systems Biology, Seattle, WA 98103, USA
    Proc Natl Acad Sci U S A 105:2562-7. 2008
    ..This activation is mediated by cytosolic flagellin through the activity of the virulence-associated type III secretion system (T3SS)...
  61. pmc Identification and functional characterization of chicken toll-like receptor 5 reveals a fundamental role in the biology of infection with Salmonella enterica serovar typhimurium
    Muhammad Iqbal
    Division of Immunology and Pathology, Institute for Animal Health, Compton, Newbury, Berkshire RG20 7NN, United Kingdom
    Infect Immun 73:2344-50. 2005
    ..Exposure of chTLR5+ cells to flagellin induced elevated levels of chicken interleukin-1beta (chIL-1beta) but little upregulation of chIL-6 mRNA...
  62. ncbi A West Nile virus recombinant protein vaccine that coactivates innate and adaptive immunity
    William F McDonald
    VaxInnate Corporation, New Haven, CT 06511, USA
    J Infect Dis 195:1607-17. 2007
    ..of delivering both innate and adaptive immune signals was designed by fusing a modified version of bacterial flagellin (STF2 Delta ) to the EIII domain of the WNV envelope protein...
  63. pmc Flagellin suppresses epithelial apoptosis and limits disease during enteric infection
    Matam Vijay-Kumar
    Department of Pathology and Laboratory Medicine, Epithelial Pathobiology Unit, Emory University School of Medicine, Atlanta, GA 30322, USA
    Am J Pathol 169:1686-700. 2006
    b>Flagellin, the primary component of bacterial flagella, is a potent activator of toll-like receptor 5 (TLR5) signaling and is a major proinflammatory determinant of enteropathogenic Salmonella...
  64. ncbi The Toll-like receptor 5 stimulus bacterial flagellin induces maturation and chemokine production in human dendritic cells
    Terry K Means
    Center for Immunology and Inflammatory Diseases and Division of Rheumatology, Allergy and Immunology, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02129, USA
    J Immunol 170:5165-75. 2003
    ..The innate immune response to bacterial flagellin is mediated by TLR5, which is expressed on human DCs...
  65. ncbi Direct stimulation of human T cells via TLR5 and TLR7/8: flagellin and R-848 up-regulate proliferation and IFN-gamma production by memory CD4+ T cells
    Gersende Caron
    Unité Mixte Institut National de la Santé et de la Recherche Médicale 564, University Hospital, Angers, France
    J Immunol 175:1551-7. 2005
    ..We report that, in the absence of APCs, flagellin (a TLR5 ligand) and R-848 (a TLR7/8 ligand) synergized with suboptimal concentrations of TCR-dependent (anti-CD3 ..
  66. ncbi Injection of flagellin into the host cell cytosol by Salmonella enterica serotype Typhimurium
    Yao Hui Sun
    Department of Medical Microbiology and Immunology, University of California at Davis, One Shields Avenue, Davis, CA 95616, USA
    J Biol Chem 282:33897-901. 2007
    ..Although the structural basis of TLR5-flagellin interaction is known, little is known about how flagellin enters the host cell cytosol to induce signaling via ..
  67. pmc Flagellin induces innate immunity in nonhost interactions that is suppressed by Pseudomonas syringae effectors
    Xinyan Li
    National Institute of Biological Sciences, Beijing 102206, China
    Proc Natl Acad Sci U S A 102:12990-5. 2005
    ..Here we show that NHO1 is transcriptionally activated by flagellin. The nonhost bacterium P. syringae pv...
  68. ncbi Flagellin perception: a paradigm for innate immunity
    Lourdes Gomez-Gomez
    Sección de Biotecnología, IDR, Campus Universitario s n, E 02071 Albacete, Spain
    Trends Plant Sci 7:251-6. 2002
    ..One of these general elicitors is flagellin, the main component of the bacterial flagellum...
  69. pmc A rapid change in virulence gene expression during the transition from the intestinal lumen into tissue promotes systemic dissemination of Salmonella
    Sebastian E Winter
    Department of Medical Microbiology and Immunology, School of Medicine, University of California at Davis, Davis, California, USA
    PLoS Pathog 6:e1001060. 2010
    ..TviA repressed expression of flagellin, a pathogen associated molecular pattern (PAMP), when bacteria were grown at osmotic conditions encountered in ..
  70. ncbi Vaccination with recombinant fusion proteins incorporating Toll-like receptor ligands induces rapid cellular and humoral immunity
    James W Huleatt
    VaxInnate Corporation, New Haven, CT 06511, USA
    Vaccine 25:763-75. 2007
    ..To test this hypothesis, we employed a recombinant fusion protein strategy using the TLR5 ligand flagellin fused to specific antigens to promote protective immunity...
  71. pmc Activation of Toll-like receptor 5 on breast cancer cells by flagellin suppresses cell proliferation and tumor growth
    Zhenyu Cai
    Center for Infectious and Inflammatory Diseases, Institute of Biosciences and Technology, Texas A and M University Health Science Center, Houston, Texas, USA
    Cancer Res 71:2466-75. 2011
    ..Interestingly, flagellin/TLR5 signaling in breast cancer cells inhibits cell proliferation and an anchorage-independent growth, a ..
  72. pmc Control of Pseudomonas aeruginosa in the lung requires the recognition of either lipopolysaccharide or flagellin
    Reuben Ramphal
    Unité de Défense Innée et Inflammation, Institut Pasteur, Paris, France
    J Immunol 181:586-92. 2008
    ..aeruginosa mutant devoid of flagellin production...
  73. pmc A novel factor controlling bistability in Bacillus subtilis: the YmdB protein affects flagellin expression and biofilm formation
    Christine Diethmaier
    Department of General Microbiology, Georg August University Gottingen, Grisebachstr 8, 37077 Gottingen, Germany
    J Bacteriol 193:5997-6007. 2011
    ..subtilis. We observed a strong overexpression of the hag gene encoding flagellin and of other genes of the σ(D)-dependent motility regulon in the ymdB mutant, whereas the two major operons for ..
  74. ncbi Flagellin promotes myeloid differentiation factor 88-dependent development of Th2-type response
    Arnaud Didierlaurent
    Swiss Institute for Experimental Cancer Research and Institute of Biochemistry, University of Lausanne, Epalinges, Switzerland
    J Immunol 172:6922-30. 2004
    ..In this study we show that flagellin, the bacterial stimulus for TLR5, drives MyD88-dependent Th2-type immunity in mice...
  75. pmc Bacterial flagellin stimulates Toll-like receptor 5-dependent defense against vancomycin-resistant Enterococcus infection
    Melissa A Kinnebrew
    Infectious Diseases Service, Department of Medicine, Immunology Program, Memorial Sloan Kettering Cancer Center, New York, New York, USA
    J Infect Dis 201:534-43. 2010
    ..Here, we demonstrate that the TLR5 agonist flagellin restores antibiotic-impaired innate immune defenses and restricts colonization with VRE...
  76. ncbi Bacterial flagellins: mediators of pathogenicity and host immune responses in mucosa
    Hugo Cruz Ramos
    Equipe AVENIR INSERM d Immunité Anti microbienne des Muqueuses, E0364, Institut Pasteur de Lille, Institut de Biologie, 1 rue du Professeur Calmette, BP 447 59021 Lille, France
    Trends Microbiol 12:509-17. 2004
    ..b>Flagellin is the structural protein that forms the major portion of flagellar filaments...
  77. pmc A bacterial flagellin, Vibrio vulnificus FlaB, has a strong mucosal adjuvant activity to induce protective immunity
    Shee Eun Lee
    National Research Laboratory of Molecular Microbial Pathogenesis and Department of Microbiology, Chonnam National University Medical School, 5 Hak dong, Dong Ku, Gwangju 501 746, South Korea
    Infect Immun 74:694-702. 2006
    b>Flagellin, the structural component of flagellar filament in various locomotive bacteria, is the ligand for Toll-like receptor 5 (TLR5) of host cells...
  78. ncbi Bacterial flagellin is an effective adjuvant for CD4+ T cells in vivo
    Stephen J McSorley
    Department of Microbiology, Center for Immunology, University of Minnesota Medical School, Minneapolis MN 55455, USA
    J Immunol 169:3914-9. 2002
    b>Flagellin is secreted by many enteric bacteria and, upon reaching the basolateral membrane of the intestinal epithelium, activates Toll-like receptor 5-mediated innate immune signaling pathways...
  79. pmc Pathophysiological role of Toll-like receptor 5 engagement by bacterial flagellin in colonic inflammation
    Sang Hoon Rhee
    Division of Gastroenterology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA
    Proc Natl Acad Sci U S A 102:13610-5. 2005
    Commensal and enteroinvasive microbes in the human gut release bacterial flagellin, a specific microbial ligand of Toll-like receptor 5 (TLR5)...
  80. ncbi Cutting edge: bacterial flagellin activates basolaterally expressed TLR5 to induce epithelial proinflammatory gene expression
    A T Gewirtz
    Epithelial Pathobiology Division, Department of Pathology and Laboratory Medicine, Emory University, Atlanta, GA 30322, USA
    J Immunol 167:1882-5. 2001
    b>Flagellin, the structural component of bacterial flagella, is secreted by pathogenic and commensal bacteria. Flagellin activates proinflammatory gene expression in intestinal epithelia...
  81. ncbi Molecular identification and characterization of the tomato flagellin receptor LeFLS2, an orthologue of Arabidopsis FLS2 exhibiting characteristically different perception specificities
    Silke Robatzek
    Zurich Basel Plant Science Center, Botanical Institute, University Basel, Hebelstrasse 1, Basel, 4056, Switzerland
    Plant Mol Biol 64:539-47. 2007
    Bacterial flagellin is known to stimulate host immune responses in mammals and plants...
  82. pmc Both the extracellular leucine-rich repeat domain and the kinase activity of FSL2 are required for flagellin binding and signaling in Arabidopsis
    L Gomez-Gomez
    Friedrich Miescher Institut, P O Box 2543, CH 4002 Basel, Switzerland
    Plant Cell 13:1155-63. 2001
    In Arabidopsis, activation of defense responses by flagellin is triggered by the specific recognition of the most conserved domain of flagellin, represented by the peptide flg22, in a process involving the FLS2 gene, which encodes a ..
  83. ncbi Critical role for Ipaf in Pseudomonas aeruginosa-induced caspase-1 activation
    Luigi Franchi
    Department of Pathology and Comprehensive Cancer Center, The University of Michigan Medical School, Ann Arbor, MI 48109, USA
    Eur J Immunol 37:3030-9. 2007
    ..aeruginosa lung infection. Further analysis showed that bacterial flagellin was essential for caspase-1 and IL-1beta and this activity depended on Ipaf and the adaptor ASC but not TLR5...
  84. pmc Topical flagellin protects the injured corneas from Pseudomonas aeruginosa infection
    Ashok Kumar
    Departments of Ophthalmology and Anatomy and Cell Biology, Kresge Eye Institute, Wayne State University School of Medicine, 4717 St Antoine, Detroit, MI 48201, USA
    Microbes Infect 12:978-89. 2010
    ..Using a mouse model of Pseudomonas keratitis, we evaluated the protective effects of topical application of flagellin, a ligand for Toll-Like receptor 5 (TLR5), on the development of Pseudomonas keratitis and elucidated the ..
  85. ncbi Studies of the dynamic expression of the Xenorhabdus FliAZ regulon reveal atypical iron-dependent regulation of the flagellin and haemolysin genes during insect infection
    Gregory Jubelin
    INRA Université Montpellier 2, UMR 1133 Laboratoire EMIP, Montpellier, France
    Environ Microbiol 13:1271-84. 2011
    ..2008). In this study, we investigated the dynamics of the FliA-dependent flagellin gene fliC and FliZ-dependent haemolysin genes xaxAB during insect infection and nematode association by carrying ..
  86. ncbi Antibodies to CBir1 flagellin define a unique response that is associated independently with complicated Crohn's disease
    Stephan R Targan
    Cedars Sinai Inflammatory Bowel Disease Center, Los Angeles, California 90048, USA
    Gastroenterology 128:2020-8. 2005
    ..The flagellin, CBir1, identified by investigations in the C3H/HeJBir mouse model, has been identified as a dominant antigen ..
  87. ncbi Salmonella flagellin induces bystander activation of splenic dendritic cells and hinders bacterial replication in vivo
    Rosa Maria Salazar-Gonzalez
    Department of Medicine, Division of Gastroenterology, Hepatology, and Nutrition, McGuire Translational Research Facility, University of Minnesota Medical School, Minneapolis, MN 55455, USA
    J Immunol 179:6169-75. 2007
    Bacterial flagellin is a target of innate and adaptive immune responses during Salmonella infection...
  88. ncbi Activation of Toll-like receptor 5 decreases the attachment of human trophoblast cells to endometrial cells in vitro
    Wedad Aboussahoud
    Academic Unit of Reproductive and Developmental Medicine, The University of Sheffield, Level 4, Jessop Wing, Tree Root Walk, Sheffield S10 2SF, UK
    Hum Reprod 25:2217-28. 2010
    ....
  89. ncbi Radioresistant cells expressing TLR5 control the respiratory epithelium's innate immune responses to flagellin
    Laure Janot
    University of Orleans and CNRS, Molecular Immunology and Embryology, UMR 6218, Institut de Transgénose, Orleans, France
    Eur J Immunol 39:1587-96. 2009
    Bacterial products (such as endotoxins and flagellin) trigger innate immune responses through TLRs. Flagellin-induced signalling involves TLR5 and MyD88 and, according to some reports, TLR4...
  90. ncbi Toll-like receptor 5 forms asymmetric dimers in the absence of flagellin
    Kaifeng Zhou
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520, USA
    J Struct Biol 177:402-9. 2012
    ..The asymmetry of the dimer suggests that TLR5 may recognize two flagellin molecules cooperatively to establish an optimal flagellin response threshold...
  91. pmc TLR5-deficient mice lack basal inflammatory and metabolic defects but exhibit impaired CD4 T cell responses to a flagellated pathogen
    Shirdi E Letran
    Center for Infectious Diseases and Microbiology Translational Research, McGuire Translational Research Facility, University of Minnesota Medical School, Minneapolis, MN 55455, USA
    J Immunol 186:5406-12. 2011
    ..In contrast, the absence of TLR5 hindered the initial activation and clonal expansion of intestinal flagellin-specific CD4 T cells following oral Salmonella infection...
  92. pmc TLR5 activation induces secretory interleukin-1 receptor antagonist (sIL-1Ra) and reduces inflammasome-associated tissue damage
    F A Carvalho
    Department of Pathology, Emory University School of Medicine, Atlanta, Georgia, USA
    Mucosal Immunol 4:102-11. 2011
    Toll-like receptor-5 (TLR5)-mediated detection of flagellin induces nuclear factor (NF)-κB-mediated transcription of host defense gene expression, whereas recognition of intracellular flagellin by interleukin (IL)-1-converting enzyme ..
  93. ncbi Helicobacter pylori HP0518 affects flagellin glycosylation to alter bacterial motility
    Hiroshi Asakura
    Department of Molecular Biology and Core Facilities for Genomics, Max Planck Institute for Infection Biology, Chariteplatz 1, Campus Chárite, 10117 Berlin, Germany
    Mol Microbiol 78:1130-44. 2010
    ..We show that the mutant's modulated motility is linked to increased levels of O-linked glycosylation on flagellin A (FlaA) protein. Recombinant HP0518 protein decreased glycosylation levels of H...
  94. pmc TLR5 functions as an endocytic receptor to enhance flagellin-specific adaptive immunity
    Shirdi E Letran
    Center for Infectious Diseases and Microbiology Translational Research, Department of Medicine, Division of Gastroenterology, Hepatology and Nutrition, McGuire Translational Research Facility, University of Minnesota Medical School, Minneapolis, MN 55455, USA
    Eur J Immunol 41:29-38. 2011
    ..we demonstrate a previously unknown function for TLR5, namely that it enhances MHC class-II presentation of flagellin epitopes to CD4(+) T cells and is required for induction of robust flagellin-specific adaptive immune responses...
  95. ncbi Flagellin enhances NK cell proliferation and activation directly and through dendritic cell-NK cell interactions
    Hironori Tsujimoto
    Department of Surgery, University of Florida College of Medicine, Gainesville, FL 32610 0286, USA
    J Leukoc Biol 78:888-97. 2005
    b>Flagellin, the principal component of bacterial flagella, is a ligand for Toll-like receptor 5 (TLR5) or TLR11 and contributes to systemic inflammation during sepsis through activation of dendritic cells (DCs) and other cells of the ..
  96. pmc A fusion protein vaccine containing OprF epitope 8, OprI, and type A and B flagellins promotes enhanced clearance of nonmucoid Pseudomonas aeruginosa
    Eric T Weimer
    Department of Microbiology and Immunology, Wake Forest University School of Medicine, Winston Salem, NC 27157, USA
    Infect Immun 77:2356-66. 2009
    ..aeruginosa. Intramuscular immunization with flagellins and OprI (separate) or OprI-flagellin fusion proteins generated significant antiflagellin immunoglobulin G (IgG) responses...
  97. pmc Carbon storage regulator A (CsrA(Bb)) is a repressor of Borrelia burgdorferi flagellin protein FlaB
    Ching Wooen Sze
    Department of Oral Biology, The State University of New York at Buffalo, New York 14214, USA
    Mol Microbiol 82:851-64. 2011
    ..a homologue of carbon storage regulator A) substantially inhibited the accumulation of FlaB, the major flagellin protein of B. burgdorferi...
  98. ncbi Isolation of motile and non-motile insertional mutants of Campylobacter jejuni: the role of motility in adherence and invasion of eukaryotic cells
    R Yao
    Enteric Diseases Program, Naval Medical Research Institute Annex, Rockville, Maryland 20852
    Mol Microbiol 14:883-93. 1994
    ..which is non-adherent and non-invasive, has an insertion of the kanamycin-resistance cassette into the flaA flagellin gene and has greatly reduced motility and a truncated flagellar filament typical of flaA mutants...
  99. ncbi Intranasal immunization with recombinant PspA fused with a flagellin enhances cross-protective immunity against Streptococcus pneumoniae infection in mice
    Chung Truong Nguyen
    Clinical Vaccine R and D Center, Chonnam National University Hwasun Hospital, 160 Ilsimri, Hwasun Gun, Jeonnam 519 809, South Korea
    Vaccine 29:5731-9. 2011
    ..We have previously demonstrated that the Vibrio vulnificus FlaB, a bacterial flagellin protein and agonist of TLR5, has strong mucosal adjuvant activity and induces protective immunity upon co-..
  100. ncbi NAIP and Ipaf control Legionella pneumophila replication in human cells
    Maya Vinzing
    Department of Internal Medicine Infectious Diseases and Pulmonary Medicine, Charite Universitatsmedizin Berlin, Berlin, Germany
    J Immunol 180:6808-15. 2008
    ..well as the NLR protein mIpaf (murine ICE protease-activating factor), are involved in recognition of Legionella flagellin and in restriction of bacterial replication. Human macrophages and lung epithelial cells support L...
  101. pmc Role of the C-terminal cytoplasmic domain of FlhA in bacterial flagellar type III protein export
    Tohru Minamino
    Graduate School of Frontier Biosciences, Osaka University, Suita, Osaka, Japan
    J Bacteriol 192:1929-36. 2010
    ....

Research Grants70

  1. MICROBES, DENDRITIC CELL SUBSETS AND T-CELL IMMUNITY
    Bali Pulendran; Fiscal Year: 2007
    ..tubercolosis, and the small synthetic molecules Pam3 Cys, and MALP], the TLR5 stimulus flagellin, the TLR7 stimuli imidazoquinolines, the TLR9 stimulus CpG DNA, and the TLR3 stimulus poly-I-C, as well as ..
  2. Controlling Radiation Injury by TLR5 Agonists
    Andrei V Gudkov; Fiscal Year: 2012
    ..In preliminary studies it was demonstrated that the bacterial flagellin protein, acting as an NF-?B activating agonist of Toll-like receptor 5 (TLR5), is a powerful radioprotectant and ..
  3. Development of a Recombinant Tetravalent Dengue Vaccine Based on a Subunit Envelo
    Lynda G Tussey; Fiscal Year: 2013
    ..our clinically proven vaccine platform in three formats, which differ in the site of antigen attachment to the flagellin (a TLR5 ligand)...
  4. ROLE OF MUC1 IN THE GENESIS OF ALLERGIC ASTHMA
    Kwang Chul Kim; Fiscal Year: 2010
    ..Recently we found that it is a receptor for Pseudomonas aeruginosa and binding of bacteria was mediated through flagellin resulting in tyrosine phosphorylation of its cytoplasmic tail...
  5. Combination Clostridium Difficile Toxin and Adhesin Vaccine
    Ciaran P Kelly; Fiscal Year: 2013
    ..TLRs when bound to their ligands such as flagellin can trigger innate responses as well as facilitate in the development of adaptive immunity...
  6. Role of surface proteins in sand fly colonization by Bartonella bacilliformis
    Michael F Minnick; Fiscal Year: 2013
    ..We also propose to examine the role of three putative virulence determinants [i.e., flagellin (FlaA), heme-binding protein C (HbpC) and the invasion-associated locus B (IalB) protein] in Bb's ..
  7. Counter-Regulation of TLR-Driven Mucosal Inflammation
    Erik P Lillehoj; Fiscal Year: 2010
    ..For example, TLRs with specificity for bacterial peptidoglycan (TLR2), LPS (TLR4), and flagellin (TLR5) activate signaling pathways leading to the production of proinflammatory cytokines and chemokines and the ..
  8. Combination Clostridium Difficile Toxin and Adhesin Vaccine
    Ciaran P Kelly; Fiscal Year: 2011
    ..TLRs when bound to their ligands such as flagellin can trigger innate responses as well as facilitate in the development of adaptive immunity...
  9. Mechanisms of flagellin-induced protection against microbial keratitis
    Fu Shin X Yu; Fiscal Year: 2013
    ..During the initial grant period, flagellin, the ligand of Toll-like receptor 5 (TLR5), was used to fortify corneal innate immunity and render resistance to ..
  10. Malaria vaccines modified with TLR agonist adjuvant
    Elizabeth H Nardin; Fiscal Year: 2010
    ..The proposed studies will utilize a well characterized TLR 5 agonist, flagellin, which has been used to developTLR-5 linked candidate vaccines to influenza (currently in clinical trial) and ..
  11. Stomate-based innate immunity against bacterial infection in Arabidopsis
    Maeli Melotto; Fiscal Year: 2012
    ..activated by highly conserved pathogen/microbe- associated molecular patterns (PAMPs/MAMPs), such as bacterial flagellin and lipopolysaccharides (LPSs)...
  12. THE STRESS-ACTIVATED PROTEIN KINASE PATHWAY
    John M Kyriakis; Fiscal Year: 2010
    ..pathogens, which trigger innate immune responses): bacterial lipopolysaccharide (LPS), peptidoglycan (PGN) and flagellin (FliC)...
  13. INNATE AND ADAPTIVE IMMUNITY IN IBD
    Charles O Elson; Fiscal Year: 2013
    ..disease who have seroreactivity to CBir1 flagellins to test the hypothesis that immune response to CBIr1 flagellin defines a population of patients with genetic variations of the IL-23, IL-17, and IL-22 pathways, as well as ..
  14. The role of CBir1 flagellin-specific Th17 and Th1 effector cells in colitis
    Yingzi Cong; Fiscal Year: 2012
    ..We recently identified enteric bacterial flagellin as immunodominant antigen in experimental colitis and in patients with Crohn's disease...
  15. Attenuated RhCMV Delta 10 SIV Oral Vaccine Vectors Encoding TLR5 Ligand Sequences
    ELLEN ELIZABETH SPARGER; Fiscal Year: 2013
    ..immunogen engineered as a fusion protein to include either full length coding sequence or specific domains of flagellin, the agonist for toll-like receptor (TLR)5...
  16. Signaling Mechanisms in Plant Innate Immunity
    Jen Sheen; Fiscal Year: 2012
    ..key function of innate immunity is the detection of microbe-associated molecular patterns (MAMPs, such bacterial flagellin) by pattern recognition receptors and the launch of appropriate defense responses...
  17. Inflammasome Response to Bacterial Infection
    Edward A Miao; Fiscal Year: 2013
    ..typhimurium infection. NLRC4 responds to flagellin and rod protein secreted into the macrophage cytosol...
  18. Listeria-based vaccines engineered to modulate the innate immune system
    Daniel A Portnoy; Fiscal Year: 2011
    ..cytosol and induces a DNA-dependent, AIM2-dependent inflammasome, while another strain secretes an ActA-flagellin (ActA-Fla) fusion protein and activates a NLRC4-dependent inflammasome...
  19. Efficacy and Mechanisms of GLN Dipeptide in the SICU
    Thomas R Ziegler; Fiscal Year: 2012
    ..serial blood levels of GSH, HSP-70 and -27, and GLN;b) decreases the presence in serum of the bacterial products flagellin and LPS and the adaptive immune response to these mediators;and c) improves key indices of innate/adaptive ..
  20. Intracellular Bacterial Recognition in the Drosophila Innate Immune Response
    Neal Silverman; Fiscal Year: 2012
    ..Small fragments of bacterial peptidoglycan as well as bacterial flagellin are known to stimulate the NOD-like Receptors in mammals...
  21. Airway Metalloproteinases in Mucosal Immunity
    CAROLE LYNN WILSON; Fiscal Year: 2010
    ..aeruginosa flagellin. We hypothesize that matrilysin is a key player in respiratory mucosal innate defense by generating an ..
  22. The Role of Inflammasomes in Mucosal Immunity to Intestinal Bacterial Pathogens
    Gabriel Nunez; Fiscal Year: 2013
    ..We have obtained evidence that NLRC4 senses bacterial flagellin that is introduced into the host cytosol via membrane pores formed by bacterial type III or IV secretion systems...
  23. Toll-like receptors and intestinal inflammation
    Charalabos Pothoulakis; Fiscal Year: 2010
    ..b>Flagellin, a major component of bacterial flagellar, is released from various bacteria, including commensal or ..
  24. Developing novel adjuvants for HIV vaccination
    Si Yi Chen; Fiscal Year: 2013
    ..The specific aims of this study are: 1). To test whether soluble flagellin (FliC)-protamine (P) fusion protein/siA20 complexes can potently stimulate APCs as novel adjuvants to induce ..
  25. Structure and function of prokaryotic appendages by cryo-electron tomography
    Elizabeth R Wright; Fiscal Year: 2013
    ..Determine the structural and functional implications of multi-flagellin flagella in pathogenic and non-pathogenic bacteria...
  26. Regulation of host inflammatory responses and outcome in melioidosis by TLR5
    TIMOTHY EOIN WEST; Fiscal Year: 2013
    ..We have identified a key role for Toll-like receptor 5 (TLR5), a cell surface flagellin sensor, in melioidosis...
  27. Flagellin-induced gut epithelial chemokine secretion
    Andrew T Gewirtz; Fiscal Year: 2012
    ..performed under this proposal revealed that ligation of toll-like receptor 5 (TLR5) by bacterial flagellin, the primary structural component of flagella, is a dominant means of activating innate immune signaling in ..
  28. Tolerance to TLR Ligands in the Intestinal Epithelium
    Anatoly V Grishin; Fiscal Year: 2010
    ..responsible for enterocyte tolerance and cross-tolerance to the ligands of TLR3 and TLR9, but not TLR5 ligand flagellin (Fla)...
  29. GENETIC ANALYSIS OF THE PLANT DEFENSE RESPONSE
    Frederick M Ausubel; Fiscal Year: 2013
    ..A high throughput Arabidopsis seedling assay allowed us to make several important discoveries. First, bacterial flagellin (Flg22) elicits the deposition of callose in cotyledons via a pathway that involves the transcription factor ..
  30. Unsaturated glycoceramide as novel platform for mucosal vaccine and drug delivery
    Wayne Lencer; Fiscal Year: 2011
    ..peptide-1 (GLP1), which acts to regulate blood sugar (Aim 1);and to the TLR5-agonist FliC (Salmonella flagellin), which we use here to model a mucosal vaccine adjuvant (Aim 2)...
  31. POTENTIATING AN HIV DNA VACCINE WITH FLAGELLIN AT THE CERVICAL/VAGINAL MUCOSA
    Dinesh Singh; Fiscal Year: 2011
    ..Therefore, the goal of this project is to evaluate flagellin to potentiate a plasmid DNA that expresses env, gag and pol genes of HIV...
  32. Development of a flagellin-cocaine conjugate vaccine
    Steven B Mizel; Fiscal Year: 2010
    ..We propose to prepare cocaine analog: flagellin conjugates that will take advantage of the potent adjuvant effect and immunogenicity of flagellin to elicit ..
  33. Role of caspases in Legionella pneumophila pulmonary infection
    Amal O Amer; Fiscal Year: 2013
    ..Ipaf is a NOD protein that senses intracellular bacterial flagellin leading to activation of caspase-1...
  34. Role of Adaptive Immunity in the Development of Colitis in TLR5 Deficient Mice
    Matam Vijay Kumar; Fiscal Year: 2013
    ..Bacterial protein flagellin, the monomeric subunit of flagella, is a dominant innate immune activator of intestinal epithelial cells...
  35. B. subtilis Reduces Intestinal Inflammation via TLR Signaling and B1 Lymphocytes
    SARA ELIZABETH JONES; Fiscal Year: 2012
    ..subtilis. Further, we discovered that B. subtilis mutants unable to synthesize either an exopolysaccharide or flagellin do not protect the host from C. rodentium-associated disease...
  36. Mechanisms of Inflammasome Inhibition by Salmonella
    Igor E Brodsky; Fiscal Year: 2013
    ..Activation of inflammasomes by Salmonella involves sensing of bacterial flagellin by NLRC4, and sensing of an unknown bacterial signal by NLRP3...
  37. High Throughput Immunoassay for Flagellin
    Eva King; Fiscal Year: 2013
    ..Recent studies in a mouse model have shown that the bacterial protein, flagellin, promotes allergic sensitization to indoor allergens and that human patients with asthma have high levels of ..
  38. Dynamic regulation of epithelial cell survival by enteropathogenic E. coli
    V Viswanathan; Fiscal Year: 2009
    ..EPEC also produces other virulence factors including flagellin and bundle-forming pili...
  39. DEVELOPMENT OF A LIVE ORAL CHOLERA VACCINE
    James B Kaper; Fiscal Year: 2010
    ..In the next period of support, we will pursue four specific aims. The first aim will be to examine the role of flagellin in TLR5 activation and IL-8 production by V. cholerae...
  40. Design of HIV VLPs with Enhanced Immunogenicity
    Richard W Compans; Fiscal Year: 2013
    ..immunogenicity will be enhanced by incorporation of molecular adjuvants, including a membrane -anchored form of flagellin, the natural ligand of the toll-like receptor 5 (TLR5), or a membrane- anchored form of GM-CSF...
  41. Innate Immune Defense against Clostridium Difficile Infection
    Eric G Pamer; Fiscal Year: 2013
    ..difficile infection in antibiotic treated mice and demonstrated that stimulating TLR-5 with bacterial flagellin or TLR7/8 with resiquimod markedly enhances resistance to C. difficile infection...
  42. Recombinant Lactobacillus as an Oral Mucosal Vaccine Against HIV-1
    Gregg A Dean; Fiscal Year: 2012
    ..gasseri after oral immunization. We will then refine L. gasseri as an immunogen by co-expressing flagellin as a means to stimulate the innate immune system through TLR5 and determining whether L...
  43. CD180 and the Macrophage Response to Legionella pneumophila
    Thomas R Hawn; Fiscal Year: 2013
    ..greater replication in CD180-/- mice compared to C57BL/6 controls over a range of MOIs in both wildtype and flagellin-deficient Lp strains...
  44. Protectan CBLB502
    Andrei Gudkov; Fiscal Year: 2009
    ..CBLB502, a novel recombinant protein derived from Salmonella FliC flagellin, is a powerful anti- radiation drug that fits those requirements...
  45. TLR Ectodomains for Microbial Detection and Therapeutics
    Neal Silverman; Fiscal Year: 2013
    ..human and 13 mouse TLRs have been characterized and shown to recognize microbial products such as lipopeptides, flagellin, lipoteichoic acid, GPI anchors, various nucleic acids, and lipopolysaccharides...
  46. Flagellin Stimulates Lung Innate Mucosal Immunity
    Theodore J Standiford; Fiscal Year: 2013
    ..by selected toll like receptors (TLR), including TLR4, which recognizes LPS, and TLR5, which is activated by flagellin. Flagellin stimulates protective immunity against diverse microbial pathogens, including bacteria and viruses...
  47. Th17 cell regulation of intestinal IgA production
    Yingzi Cong; Fiscal Year: 2010
    ..We have generated a TCR transgenic mouse line that is specific for CBir1 flagellin, one of these commensal bacterial flagellins. We have crossed CBir1 Tg mice with IL-17F-Thy1...
  48. Deconstructing Inflammation and Altered Microbiota In Metabolic Syndrome
    Andrew T Gewirtz; Fiscal Year: 2013
    ..Our study of mice with a discrete innate immune deficiency, namely loss of the flagellin receptor toll-like receptor 5 (TLR5), led us to hypothesize that an overarching mechanism by which an aberrant ..
  49. Control of intestinal microflora and inflammation by immune sensing of flagellin
    Janelle S Ayres; Fiscal Year: 2012
    ..suggests that the commensal bacteria appear to be essential for the development of CD and the bacterial protein, flagellin, has been found to be a dominant antigen in CD patients...
  50. Vaccination strategies to overcome immune deficiencies in neonates
    MARTHA ANN ALEXANDER-MILLER; Fiscal Year: 2013
    ..Aim1. To determine the ability of R848 and flagellin to modulate the humoral and cell mediated vaccine responses in neonates...
  51. Design and function of flagellin-based immunogens for broadly neutralizing immuni
    Kelly D Smith; Fiscal Year: 2013
    ..To address this hypothesis we propose to engineer flagellin molecules that will display HIV epitopes and posses potent adjuvant activity, and thus transform bacterial ..
  52. Pathophysiology of TLR5KO Colitis
    Andrew T Gewirtz; Fiscal Year: 2013
    ..The bacterial protein flagellin, the monomeric subunit of flagella, is a dominant innate immune activator of intestinal epithelial cells...
  53. Overcoming Immune Suppression to Enhance Tumor Vaccine Efficacy
    Si Yi Chen; Fiscal Year: 2013
    ..DCs that are transduced to coexpress the inhibitor of TLR's negative regulator A20 (shA20) and secretory flagellin (FliC), TLR5 ligand, to uniquely trigger and sustain TLR signaling cascades can efficiently stimulate the ..
  54. Consequences of NLRC4/NAIP5 Inflammasome Signaling on Adaptive Immunity and Tumor
    ANDREW T KENT; Fiscal Year: 2013
    ..Furthermore, our laboratory has recently shown that artificial expression of the TLR and NLR agonist flagellin within tumor cells prevented their growth once implanted into recipient mice (Garaude et al...
  55. Specificity and in vivo function of the Naip/Nlrc4 inflammasomes
    Russell E Vance; Fiscal Year: 2013
    ..In the past funding period, we found that Naip5 mediates specific cytosolic detection of bacterial flagellin, whereas Naip2 mediates specific cytosolic detection of the inner rod protein from diverse bacterial type III ..
  56. Protection from enteric pathogens by beneficial microbes
    Katherine L Knight; Fiscal Year: 2013
    ..rodentium. One of the non-protective B. subtilis mutants is deficient in flagellin, a ligand for TLR5, and another is deficient in exopolysaccharide, presumed ligand for TLR2, and in Aim 2 we ..
  57. Innate immune response to bacterial flagellins
    Stephen J McSorley; Fiscal Year: 2013
    ..we have found that the innate receptor TLR5 is essential for the development of a robust T cell response to flagellin, but that this requires an unconventional signaling pathway that has not previously been examined...
  58. Flagellin regulation by Vibrio fischeri during colonization and presistence
    TIMOTHY MIYASHIRO; Fiscal Year: 2010
    ..The specific aims of this research application are: 1) to characterize each type of flagellin during initial colonization of the squid light organ;and, 2) to identify the bacterial determinants, including ..
  59. Phosphorylation and ubiquitination of immune sensory complexes in innate immune s
    Libo Shan; Fiscal Year: 2013
    ..senor FLS2, a structural and functional ortholog of mammalian Toll-like receptors (TLR), recognizes bacterial flagellin and initiates immune signaling by association with a receptor-like kinase BAK1...
  60. Preclinical Development of Flagellin as a Mucosal Adjuvant in Biodefense Vaccines
    STEVEN MIZEL; Fiscal Year: 2009
    DESCRIPTION (provided by applicant): Gram-negative flagellin is an extraordinarily potent activator of innate and adaptive immune responses. Our work as well as that of others has established that flagellin is a potent adjuvant in mice...
  61. Novel anti-bacterial therapy for gram negative pneumonia
    Kanneganti Murthy; Fiscal Year: 2001
    ..To address this unmet need, Inotek is developing an antibody-based strategy that targets flagellin, a critical virulence factor of motile gram negative pathogens...
  62. Associations between gut microbiota, flagellin production and bone microstructure
    CHRISTOPHER JOHN HERNANDEZ; Fiscal Year: 2013
    ..The proposed work will provide the senior fellow with training in the use of a germ free facility, including transplantation of gut microbiota and analysis of the composition of the gut flora for future studies in bone. ..
  63. Autophagy as a component of the innate immune response
    MICHELE SWANSON; Fiscal Year: 2009
    ..pneumophila infection by relying on NOD-like proteins to detect cytosolic flagellin and then to digest the microbes by autophagy or to commit a pro-inflammatory cell death known as pyroptosis...
  64. IMMUNOBIOLOGY OF CAMPYLOBACTER JEJUNI
    Irving Nachamkin; Fiscal Year: 1990
    ..Campylobacter flagellin, the major protein of the flagella filament, is the major immunodominant protein of Campylobacter jejuni in ..
  65. STRUCTURAL STUDIES OF BACTERIAL FLAGELLA
    David Derosier; Fiscal Year: 1999
    ..The propellor is a helically shaped filament made up of flagellin subunits. Rotation of the motor turns the filament which in turn propels the cell...
  66. DNA TOPOISOMERASES--BIOLOGICAL FUNCTIONS AND MECHANISMS
    James Wang; Fiscal Year: 1999
    ..motor, located in the cell's inner membrane, land a propeller, which is a helically shaped filament made up of flagellin subunits...
  67. PSEUDOMONAS AERUGINOSA FLAGELLAR BIOGENESIS PATHWAY
    Reuben Ramphal; Fiscal Year: 2007
    ..Recent findings demonstrate that growth of P. aeruginosa in respiratory mucus results in a block in flagellin synthesis...
  68. Generation of Asymmtery During Caulobacter Development
    JAMES GOBER; Fiscal Year: 2005
    ..translation, we describe experiments designed to identify the factors that regulate FlbT and the translation of flagellin genes. Finally, w e propose to identify genes outside of the flagellar regulon that are also controlled by FIbD...
  69. Innate immune resistance to intracellular bacterial pathogens
    RUSSELL VANCE; Fiscal Year: 2007
    ..depends on activation of a rapid caspase-1-mediated cell death that is triggered upon the detection of bacterial flagellin in the macrophage cytosol. Thus, the specific aims of this grant proposal are: 1...
  70. Flagellar Phase Variation in Salmonella
    Kelly Hughes; Fiscal Year: 2005
    ..H.O. estimates). Salmonella enterica alternatively expresses one of two antigenically distinct flagellin proteins, FliC or FljB...