factor for inversion stimulation protein

Summary

Summary: A highly abundant DNA binding protein whose expression is strongly correlated with the growth phase of bacteria. The protein plays a role in regulating DNA topology and activation of RIBOSOMAL RNA transcription. It was originally identified as a factor required for inversion stimulation by the Hin recombinase of SALMONELLA and Gin site-specific recombinase of BACTERIOPHAGE MU.

Top Publications

  1. ncbi Involvement of FIS in the H-NS-mediated regulation of virF gene of Shigella and enteroinvasive Escherichia coli
    M Falconi
    Laboratorio di Genetica, Dipartimento di Biologia MCA, , 62032 Camerino (MC, Italy
    Mol Microbiol 42:439-52. 2001
  2. ncbi Transient repressor effect of Fis on the growth phase-regulated osmE promoter of Escherichia coli K12
    P Bordes
    Laboratoire de Microbiologie et Genetique Moleculaire, UMR 5100 CNRS Université Toulouse III, 118 route de Narbonne, F 31062 Toulouse Cedex, France
    Mol Genet Genomics 268:206-13. 2002
  3. pmc Growth phase-dependent variation in protein composition of the Escherichia coli nucleoid
    T Ali Azam
    Department of Molecular Genetics, National Institute of Genetics, Mishima, Shizuoka 411 8540, Japan
    J Bacteriol 181:6361-70. 1999
  4. pmc The shape of the DNA minor groove directs binding by the DNA-bending protein Fis
    Stefano Stella
    Department of Biological Chemistry, David Geffen School of Medicine at the University of California at Los Angeles, 90095 1737, USA
    Genes Dev 24:814-26. 2010
  5. pmc Association of nucleoid proteins with coding and non-coding segments of the Escherichia coli genome
    David C Grainger
    School of Biosciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK
    Nucleic Acids Res 34:4642-52. 2006
  6. ncbi A global role for Fis in the transcriptional control of metabolism and type III secretion in Salmonella enterica serovar Typhimurium
    Arlene Kelly
    Department of Microbiology, Moyne Institute of Preventive Medicine, University of Dublin, Trinity College, Dublin 2, Ireland
    Microbiology 150:2037-53. 2004
  7. pmc Mechanism of chromosome compaction and looping by the Escherichia coli nucleoid protein Fis
    Dunja Skoko
    University of Illinois at Chicago, Department of Physics, Chicago, IL 60607 7059, USA
    J Mol Biol 364:777-98. 2006
  8. pmc Dramatic changes in Fis levels upon nutrient upshift in Escherichia coli
    C A Ball
    Molecular Biology Institute, University of California, Los Angeles 90024
    J Bacteriol 174:8043-56. 1992
  9. pmc DksA is required for growth phase-dependent regulation, growth rate-dependent control, and stringent control of fis expression in Escherichia coli
    Prabhat Mallik
    Department of Biological Sciences, University at Albany, 1400 Washington Avenue, Albany, NY 12222, USA
    J Bacteriol 188:5775-82. 2006
  10. pmc Information analysis of Fis binding sites
    P N Hengen
    Laboratory of Mathematical Biology, National Cancer Institute, Frederick Cancer Research and Development Center, PO Box B, Building 469, Room 144, Frederick, MD 21702 1201, USA
    Nucleic Acids Res 25:4994-5002. 1997

Scientific Experts

Detail Information

Publications133 found, 100 shown here

  1. ncbi Involvement of FIS in the H-NS-mediated regulation of virF gene of Shigella and enteroinvasive Escherichia coli
    M Falconi
    Laboratorio di Genetica, Dipartimento di Biologia MCA, , 62032 Camerino (MC, Italy
    Mol Microbiol 42:439-52. 2001
    ..Our data indicate that FIS may be relevant for the rapid increase in virF expression after penetration of bacteria into the host...
  2. ncbi Transient repressor effect of Fis on the growth phase-regulated osmE promoter of Escherichia coli K12
    P Bordes
    Laboratoire de Microbiologie et Genetique Moleculaire, UMR 5100 CNRS Université Toulouse III, 118 route de Narbonne, F 31062 Toulouse Cedex, France
    Mol Genet Genomics 268:206-13. 2002
    ..In contrast, expression of osmE (p) at elevated osmolarity during the mid-exponential growth phase is increased in the absence of Fis, demonstrating that Fis is able to act as a repressor in vivo at a particular stage of growth...
  3. pmc Growth phase-dependent variation in protein composition of the Escherichia coli nucleoid
    T Ali Azam
    Department of Molecular Genetics, National Institute of Genetics, Mishima, Shizuoka 411 8540, Japan
    J Bacteriol 181:6361-70. 1999
    ..These changes in the composition of nucleoid-associated proteins in the stationary phase are accompanied by compaction of the genome DNA and silencing of the genome functions...
  4. pmc The shape of the DNA minor groove directs binding by the DNA-bending protein Fis
    Stefano Stella
    Department of Biological Chemistry, David Geffen School of Medicine at the University of California at Los Angeles, 90095 1737, USA
    Genes Dev 24:814-26. 2010
    ..Then Fis further compresses the minor groove and bends the DNA to generate the bound structure...
  5. pmc Association of nucleoid proteins with coding and non-coding segments of the Escherichia coli genome
    David C Grainger
    School of Biosciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK
    Nucleic Acids Res 34:4642-52. 2006
    ..Hence some targets are associated with combinations of bound FIS, H-NS and IHF. In addition, many regions associated with FIS and H-NS are also associated with RNA polymerase...
  6. ncbi A global role for Fis in the transcriptional control of metabolism and type III secretion in Salmonella enterica serovar Typhimurium
    Arlene Kelly
    Department of Microbiology, Moyne Institute of Preventive Medicine, University of Dublin, Trinity College, Dublin 2, Ireland
    Microbiology 150:2037-53. 2004
    ..The data reported reveal the central role played by Fis in coordinating the expression of both housekeeping and virulence factors required by S. typhimurium during life in the gut lumen or during systemic infection of host cells...
  7. pmc Mechanism of chromosome compaction and looping by the Escherichia coli nucleoid protein Fis
    Dunja Skoko
    University of Illinois at Chicago, Department of Physics, Chicago, IL 60607 7059, USA
    J Mol Biol 364:777-98. 2006
    ..These results suggest that Fis may play a role in vivo as a domain barrier element by organizing DNA loops within the E. coli chromosome...
  8. pmc Dramatic changes in Fis levels upon nutrient upshift in Escherichia coli
    C A Ball
    Molecular Biology Institute, University of California, Los Angeles 90024
    J Bacteriol 174:8043-56. 1992
    ..We suggest that the fluctuations in Fis levels may serve as an early signal of a nutritional upshift and may be important in the physiological roles Fis plays in the cell...
  9. pmc DksA is required for growth phase-dependent regulation, growth rate-dependent control, and stringent control of fis expression in Escherichia coli
    Prabhat Mallik
    Department of Biological Sciences, University at Albany, 1400 Washington Avenue, Albany, NY 12222, USA
    J Bacteriol 188:5775-82. 2006
    ..This work extends our understanding of the multiple factors controlling fis expression and demonstrates the generality of the DksA requirement for regulation of kinetically similar promoters...
  10. pmc Information analysis of Fis binding sites
    P N Hengen
    Laboratory of Mathematical Biology, National Cancer Institute, Frederick Cancer Research and Development Center, PO Box B, Building 469, Room 144, Frederick, MD 21702 1201, USA
    Nucleic Acids Res 25:4994-5002. 1997
    ..A sequence in aldB previously reported to be a Fis site, for which information theory predicts no site, did not shift. These results demonstrate that information analysis is useful for predicting Fis DNA binding...
  11. ncbi Effects of Fis on Escherichia coli gene expression during different growth stages
    Meranda D Bradley
    Department of Biological Science, University at Albany, 1400 Washington Avenue, Albany, NY 12222, USA
    Microbiology 153:2922-40. 2007
    ..The results demonstrate that the growth phase-dependent Fis expression triggers coordinate regulation of 15 categories of functionally related genes during specific stages of growth of an E. coli culture...
  12. pmc Genome-wide analysis of Fis binding in Escherichia coli indicates a causative role for A-/AT-tracts
    Byung Kwan Cho
    Department of Bioengineering, University of California San Diego, La Jolla, California 92093 0412, USA
    Genome Res 18:900-10. 2008
    ..These novel results provide new insight into how Fis modulates DNA topology at a genome scale and thus advance our understanding of the architectural bases of the E. coli nucleoid...
  13. ncbi Escherichia coli prereplication complex assembly is regulated by dynamic interplay among Fis, IHF and DnaA
    Valorie T Ryan
    Department of Biological Sciences, Florida Institute of Technology, Melbourne, Florida 32901 6795, USA
    Mol Microbiol 51:1347-59. 2004
    ....
  14. ncbi DNA supercoiling is differentially regulated by environmental factors and FIS in Escherichia coli and Salmonella enterica
    Andrew D S Cameron
    Department of Microbiology, Moyne Institute of Preventive Medicine, School of Genetics and Microbiology, Trinity College Dublin, Dublin 2, Ireland
    Mol Microbiol 80:85-101. 2011
    ..coli and S. enterica. These findings suggest that E. coli and S. enterica may be unexpectedly divergent in their global regulation of cellular physiology...
  15. ncbi H-NS: a universal regulator for a dynamic genome
    Charles J Dorman
    Department of Microbiology, Moyne Institute of Preventive Medicine, Trinity College, University of Dublin, Dublin 2, Ireland
    Nat Rev Microbiol 2:391-400. 2004
  16. ncbi Antagonistic involvement of FIS and H-NS proteins in the transcriptional control of hns expression
    M Falconi
    Department of Biology, University of Camerino, Italy
    Mol Microbiol 19:965-75. 1996
    ....
  17. ncbi Genome organisation and chromatin structure in Escherichia coli
    D Ussery
    Center for Biological Sequence Analysis, Department of Biotechnology, Building 208, The Technical University of Denmark, 2800 Kgs Lyngby, Denmark
    Biochimie 83:201-12. 2001
    ..We also show that the regions upstream of genes regulated by H-NS are more curved and have a higher AT content than regions upstream of other genes. These regions in general would also be localised near the replication terminus...
  18. pmc Functional characterization of the Escherichia coli Fis-DNA binding sequence
    Yongping Shao
    Department of Biological Sciences, University at Albany, 1400 Washington Avenue, Albany, NY 12222, USA
    J Mol Biol 376:771-85. 2008
    ....
  19. ncbi CRP modulates fis transcription by alternate formation of activating and repressing nucleoprotein complexes
    W Nasser
    Institut fur Genetik und Mikrobiologie, Ludwig Maximilians Univesitaet, Maria Ward Strasse 1a, 80638 Munchen, Germany
    J Biol Chem 276:17878-86. 2001
    ..This cross-talk is manifested in alternate formation of functional nucleoprotein complexes exerting either activating or repressing effects on transcription...
  20. ncbi Low-force DNA condensation and discontinuous high-force decondensation reveal a loop-stabilizing function of the protein Fis
    Dunja Skoko
    Department of Physics, University of Illinois at Chicago, 845 West Taylor Street, Chicago, Illinois 60607 7059, USA
    Phys Rev Lett 95:208101. 2005
    ..Our experiments suggest that Fis may play a role in vivo stabilizing the "loop-domain" structure of the bacterial chromosome...
  21. ncbi Integration of three signals at the Escherichia coli nrf promoter: a role for Fis protein in catabolite repression
    Douglas F Browning
    School of Biosciences, The University of Birmingham, Birmingham B15 2TT, UK
    Mol Microbiol 57:496-510. 2005
    ..Hence, Fis protein is a major factor responsible for catabolite repression at the nrf promoter, and Fis can override activation by FNR and NarL or NarP...
  22. ncbi Two types of localization of the DNA-binding proteins within the Escherichia coli nucleoid
    T A Azam
    Department of Molecular Genetics, National Institute of Genetics, Mishima, Shizuoka 411 8540, Japan
    Genes Cells 5:613-26. 2000
    ..The intracellular localization of these proteins in E. coli could be predicted from these data, but no attempt has been made thus far to directly observe the intracellular distribution of the DNA-binding proteins...
  23. ncbi Suppression of FNR-dependent transcription activation at the Escherichia coli nir promoter by Fis, IHF and H-NS: modulation of transcription initiation by a complex nucleo-protein assembly
    D F Browning
    School of Biosciences, The University of Birmingham, Birmingham B15 2TT, UK
    Mol Microbiol 37:1258-69. 2000
    ..NarL and NarP can relieve both IHF- and Fis-mediated repression, but are unable to counteract H-NS-mediated repression...
  24. pmc Fis, an accessorial factor for transcriptional activation of the mar (multiple antibiotic resistance) promoter of Escherichia coli in the presence of the activator MarA, SoxS, or Rob
    R G Martin
    Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, Maryland 20892 0560, USA
    J Bacteriol 179:7410-9. 1997
    ..Thus, Fis acts as an accessory transcriptional activator at the mar promoter...
  25. pmc An architectural role of the Escherichia coli chromatin protein FIS in organising DNA
    R Schneider
    Institut fuer Genetik und Mikrobiologie, LMU Muenchen, Maria Ward Strasse 1a, D 80638 Muenchen, Germany
    Nucleic Acids Res 29:5107-14. 2001
    ..coli nucleoid...
  26. pmc Transcriptional regulation of fis operon involves a module of multiple coupled promoters
    William Nasser
    Max Planck Institute for Terrestrial Microbiology, Karl von Frisch Strasse, D 35043 Marburg, Germany
    EMBO J 21:715-24. 2002
    ..We infer that these coupled promoters represent a regulatory module and propose a novel "dynamic balance" mechanism involved in the transcriptional control of the fis operon...
  27. pmc Downregulation of the Escherichia coli guaB promoter by FIS
    Seyyed I Husnain
    F Floor, School of Medicine and Biomedical Sciences, University of Sheffield, Beech Hill Road, Sheffield S10 2RX, UK
    Microbiology 154:1729-38. 2008
    ..Binding of FIS to these sites results in repression of P(guaB) in vitro but not in vivo. Deletion of the fis gene results in increased P(guaB) activity in vivo, but GRDC of P(guaB) is maintained...
  28. ncbi Three-dimensional structure of the E. coli DNA-binding protein FIS
    D Kostrewa
    Institut fur Kristallographie, Freie Universitat Berlin, Germany
    Nature 349:178-80. 1991
    ..We infer from model building that DNA has to bend for tight binding to FIS...
  29. pmc Escherichia coli host factor for site-specific DNA inversion: cloning and characterization of the fis gene
    C Koch
    Max Planck Institut fur Molekulare Genetik, Otto Warburg Laboratorium, Berlin, Federal Republic of Germany
    Proc Natl Acad Sci U S A 85:4237-41. 1988
    ..Fis is nonessential for Es. coli growth; however, inversion of the G segment of a Mu prophage was not detected in the fis mutant. The fis gene is located between 71 and 72 min on the Es. coli genetic map...
  30. pmc The FIS protein binds and bends the origin of chromosomal DNA replication, oriC, of Escherichia coli
    H Gille
    Max Planck Institut fur Molekulare Genetik, Berlin, FRG
    Nucleic Acids Res 19:4167-72. 1991
    ..A fis mutant strain can not be efficiently transformed with plasmids which carry and replicate from oriC, suggesting that FIS is required for minichromosome replication...
  31. ncbi Growth phase-dependent regulation of nuoA-N expression in Escherichia coli K-12 by the Fis protein: upstream binding sites and bioenergetic significance
    B Wackwitz
    Institut für Mikrobiologie und Weinforschung, Universitat Mainz, Germany
    Mol Gen Genet 262:876-83. 1999
    ..This ensures higher ATP yields under conditions where large amounts of ATP are required...
  32. ncbi Selective regulation of ptsG expression by Fis. Formation of either activating or repressing nucleoprotein complex in response to glucose
    Dongwoo Shin
    Department of Food Science and Technology, School of Agricultural Biotechnology and Center for Agricultural Biomaterials, Seoul National University, Suwon 441 744, Korea
    J Biol Chem 278:14776-81. 2003
    ..Tighter Mlc repression and enhanced CRP.cAMP activation of ptsG P1 by Fis enable cells to regulate Mlc regulon efficiently by selectively controlling the concentration of enzyme IICB(Glc) that modulates Mlc activity...
  33. ncbi Selective repression by Fis and H-NS at the Escherichia coli dps promoter
    David C Grainger
    School of Biosciences, University of Birmingham, Edgbaston, Birmingham, UK
    Mol Microbiol 68:1366-77. 2008
    ..Our data suggest a simple model to explain how the Dps-dependent super-compaction of the folded chromosome is triggered as cell growth ceases...
  34. pmc Regulation of crp transcription by oscillation between distinct nucleoprotein complexes
    G Gonzalez-Gil
    Institut für Genbiologische Forschung Berlin GmbH, Berlin, Germany
    EMBO J 17:2877-85. 1998
    ..Our results suggest that the control of crp transcription is achieved by oscillation in the composition of these regulatory nucleoprotein complexes in response to the physiological state of the cell...
  35. pmc Dynamic state of DNA topology is essential for genome condensation in bacteria
    Ryosuke L Ohniwa
    Laboratory of Plasma Membrane and Nuclear Signaling, Kyoto University Graduate School of Biostudies, Kitashirakawa Oiwake cho, Sakyo ku, Kyoto, Japan
    EMBO J 25:5591-602. 2006
    ..These results indicate that the topology of DNA is dynamically transmutable and that the topology control is important for Dps-induced nucleoid condensation...
  36. pmc Homeostatic regulation of supercoiling sensitivity coordinates transcription of the bacterial genome
    Nicolas Blot
    International University Bremen, Campus Ring 1, 28759 Bremen, Germany
    EMBO Rep 7:710-5. 2006
    ..We infer that a homeostatic mechanism organizing the supercoiling sensitivity is coordinating the growth-phase-dependent transcription of the genome...
  37. pmc Contributions of UP elements and the transcription factor FIS to expression from the seven rrn P1 promoters in Escherichia coli
    C A Hirvonen
    Department of Bacteriology, University of Wisconsin Madison, Madison, Wisconsin 53706, USA
    J Bacteriol 183:6305-14. 2001
    ..These studies have implications for the control of gene expression of unlinked multigene families...
  38. ncbi The role of FIS in the Rcd checkpoint and stable maintenance of plasmid ColE1
    I K Blaby
    Department of Genetics, University of Cambridge, Cambridge CB2 3EH, UK
    Microbiology 155:2676-82. 2009
    ..Rcd transcription is triggered in plasmid dimers by the lifting of XerCD-mediated repression in the synaptic complex...
  39. pmc Promoter protection by a transcription factor acting as a local topological homeostat
    Mark Rochman
    Max Planck Institute for Terrestrial Microbiology, Karl von Frisch Strasse, D 35043 Marburg, Germany
    EMBO Rep 3:355-60. 2002
    ..We infer that FIS maintains stable RNA transcription by stabilizing local writhe in the UAS. These results suggest a novel mechanism of transcriptional regulation by a transcription factor acting as a local topological homeostat...
  40. ncbi Role of the nucleoid-associated protein Fis in the regulation of virulence properties of enteropathogenic Escherichia coli
    M D Goldberg
    Department of Microbiology and Immunology, School of Medicine, Maurice Shock Building, PO Box 138, University Road, Leicester LE1 9HN, UK
    Mol Microbiol 41:549-59. 2001
    ....
  41. ncbi A DNA architectural protein couples cellular physiology and DNA topology in Escherichia coli
    R Schneider
    Institut fur Genetik und Mikrobiologie, LMU Munchen, Maria Ward Str 1a, 80638 Munchen, Germany
    Mol Microbiol 34:953-64. 1999
    ..We propose that FIS is involved in coupling cellular physiology to the topology of the bacterial chromosome...
  42. pmc The small nucleoid-binding proteins H-NS, HU, and Fis affect hilA expression in Salmonella enterica serovar Typhimurium
    Lisa M Schechter
    Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA
    Infect Immun 71:5432-5. 2003
    ..Modulation of hilA by counteracting repressing and derepressing mechanisms may allow Salmonella serovar Typhimurium to regulate its virulence genes in response to different situations in vivo...
  43. ncbi FIS modulates the kinetics of successive interactions of RNA polymerase with the core and upstream regions of the tyrT promoter
    Iain K Pemberton
    Enzymologie et cinétique structurale, UMR 8532 du CNRS, LBPA, Ecole Normale Superieure de Cachan, 94235 Cachan, France
    J Mol Biol 318:651-63. 2002
    ..We further suggest that FIS plays a similar role and may facilitate polymerase escape...
  44. ncbi Escherichia coli response to hydrogen peroxide: a role for DNA supercoiling, topoisomerase I and Fis
    D Weinstein-Fischer
    Department of Molecular Genetics and Biotechnology, The Hebrew University Hadassah Medical School, 91120 Jerusalem, Israel
    Mol Microbiol 35:1413-20. 2000
    ..We also show that a fis deficient mutant strain is extremely sensitive to hydrogen peroxide. Our results suggest that topA activation by Fis is an important component of the Escherichia coli response to oxidative stress...
  45. pmc Identification of regulatory network topological units coordinating the genome-wide transcriptional response to glucose in Escherichia coli
    Rosa María Gutiérrez-Ríos
    Departamentos de Microbiología Molecular, Instituto de Biotecnologia, Universidad Nacional Autonoma de Mexico, Apdo Postal 510 3, Cuernavaca, Morelos, Mexico
    BMC Microbiol 7:53. 2007
    ..To determine the extent of the cellular response to glucose, we applied an approach combining global transcriptome and regulatory network analyses...
  46. ncbi Regulation of gene expression by histone-like proteins in bacteria
    Charles J Dorman
    Department of Microbiology, Moyne Institute of Preventive Medicine, University of Dublin, Trinity College, Dublin 2, Ireland
    Curr Opin Genet Dev 13:179-84. 2003
    ..Recent advances have also been assisted by insights into the effects the histone-like proteins exert on DNA structure not only at specific promoters but throughout the genome...
  47. pmc Regulation by nucleoid-associated proteins at the Escherichia coli nir operon promoter
    Douglas F Browning
    School of Biosciences, University of Birmingham, Birmingham, B15 2TT, United Kingdom
    J Bacteriol 190:7258-67. 2008
    ..Differences in the upstream IHF and Fis binding sites at the nir promoter in related enteric bacteria fix the level of nir operon expression under anaerobic growth conditions...
  48. ncbi The role of FIS protein in the physiological control of the expression of the Escherichia coli meta-hpa operon
    Beatriz Galán
    Department of Molecular Microbiology, Centro de Investigaciones Biologicas, CSIC, Madrid, Spain
    Microbiology 154:2151-60. 2008
    ..These results suggest that FIS contributes to silencing the Pg promoter in the exponential phase of growth in an undefined rich medium when FIS is predominantly expressed...
  49. ncbi DNA supercoiling and transcription in Escherichia coli: The FIS connection
    A Travers
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK
    Biochimie 83:213-7. 2001
    ..We suggest that these microloops compensate in part for the FIS-induced lowering of the superhelical density...
  50. pmc The Escherichia coli Fis protein prevents initiation of DNA replication from oriC in vitro
    S Wold
    Department of Biophysics, Institute for Cancer Research, Montebello, Oslo, Norway
    Nucleic Acids Res 24:3527-32. 1996
    ..This indicates a negative role for Fis in the regulation of replication initiation...
  51. ncbi DNA microloops and microdomains: a general mechanism for transcription activation by torsional transmission
    A Travers
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge, CB2 2QH, England
    J Mol Biol 279:1027-43. 1998
    ..In this process, which we term torsional transmission, a major function of the activator is to act as a local topological homeostat. We argue that the same mechanism may also be employed in site-specific DNA inversion...
  52. pmc FIS and RNA polymerase holoenzyme form a specific nucleoprotein complex at a stable RNA promoter
    G Muskhelishvili
    Institute für Genetik und Mikrobiologie, Munchen, Germany
    EMBO J 14:1446-52. 1995
    ..Such a mechanism could overcome transient limitations on the availability of sigma 70 or core polymerase after a prolonged stationary phase...
  53. ncbi The Fis protein: it's not just for DNA inversion anymore
    S E Finkel
    Department of Biological Chemistry, UCLA School of Medicine 90024 1737
    Mol Microbiol 6:3257-65. 1992
    ..The X-ray crystal structure of Fis has been determined and insights into its mode of DNA binding and mechanisms of action in these disparate systems are being made...
  54. ncbi Regulation of HU alpha and HU beta by CRP and FIS in Escherichia coli
    L Claret
    Institute de Biologie Physico Chimique, Laboratoire de Physiologie Bacterienne, Paris, France
    J Mol Biol 263:126-39. 1996
    ..It is rather unexpected that such divergent transcriptional regulation controls the two genes encoding a dimeric protein...
  55. ncbi The small nucleoid protein Fis is involved in Vibrio cholerae quorum sensing
    Derrick H Lenz
    Department of Molecular Biology, Princeton University, Princeton, NJ 08544 1014, USA
    Mol Microbiol 63:859-71. 2007
    ....
  56. ncbi The C-terminal domains of the RNA polymerase alpha subunits: contact site with Fis and localization during co-activation with CRP at the Escherichia coli proP P2 promoter
    Sarah M McLeod
    Department of Biological Chemistry, UCLA School of Medicine, Los Angeles, CA 90095 1737, USA
    J Mol Biol 316:517-29. 2002
    ..These experiments define the overall architecture of the proP P2 initiation complex where Fis and CRP each function through a different alphaCTD...
  57. ncbi A genetic selection for supercoiling mutants of Escherichia coli reveals proteins implicated in chromosome structure
    Christine D Hardy
    Department of Molecular and Cell Biology, 16 Barker Hall, University of California, Berkeley, CA 94720 3204, USA
    Mol Microbiol 57:1636-52. 2005
    ..We suggest that at least H-NS, Fis and perhaps TktA assist directly in the supercoiling of domains by forming topological barriers on the E. coli chromosome...
  58. ncbi The ndh-binding protein (Nbp) regulates the ndh gene of Escherichia coli in response to growth phase and is identical to Fis
    J Green
    Krebs Institute, Department of Molecular Biology and Biotechnology, University of Sheffield, UK
    Mol Microbiol 20:1043-55. 1996
    ....
  59. pmc The transactivation region of the fis protein that controls site-specific DNA inversion contains extended mobile beta-hairpin arms
    M K Safo
    Institute of Molecular Biology, Academia Sinica, Taipei, Taiwan 11529, Republic of China
    EMBO J 16:6860-73. 1997
    ..The emerging picture for this regulatory motif is that contacts with the recombinase at the tip of the mobile beta-arms activate the DNA invertase in the context of an invertasome complex...
  60. pmc The E.coli fis promoter is subject to stringent control and autoregulation
    O Ninnemann
    Institut für Genbiologische Forschung Berlin GmbH, Germany
    EMBO J 11:1075-83. 1992
    ..We show that the fis promoter is subject to stringent control and discuss this unusual feature with respect to the known and putative functions FIS serves in E. coli...
  61. pmc Identification of two functional regions in Fis: the N-terminus is required to promote Hin-mediated DNA inversion but not lambda excision
    R Osuna
    Department of Biological Chemistry, School of Medicine, University of California, Los Angeles 90024
    EMBO J 10:1593-603. 1991
    ....
  62. pmc The molecular structure of wild-type and a mutant Fis protein: relationship between mutational changes and recombinational enhancer function or DNA binding
    H S Yuan
    Molecular Biology Institute, University of California, Los Angeles 90024
    Proc Natl Acad Sci U S A 88:9558-62. 1991
    ..In contrast, mutations that affect HTH helices C and D, or interactions of these with helix B, have the additional effect of decreasing or eliminating binding to DNA...
  63. ncbi Regulation of ndh expression in Escherichia coli by Fis
    Laura Jackson
    Krebs Institute for Biomolecular Research, Department of Molecular Biology and Biotechnology, University of Sheffield, Western Bank, Sheffield S10 2TN, UK
    Microbiology 150:407-13. 2004
    ....
  64. pmc Correlation between binding rate constants and individual information of E. coli Fis binding sites
    Ryan K Shultzaberger
    National Cancer Institute at Frederick, Center for Cancer Research Nanobiology Program, SAIC Frederick, NCI Frederick Bldg 469, Rm 237 Frederick, MD 21782, USA
    Nucleic Acids Res 35:5275-83. 2007
    ..Finally, we observed a break in the binding curve around zero bits of information. The break is expected from information theory because it represents the coding demarcation between specific and nonspecific binding...
  65. pmc The promoter of the tgt/sec operon in Escherichia coli is preceded by an upstream activation sequence that contains a high affinity FIS binding site
    R K Slany
    Institut fur Biochemie, Universitat Erlangen Nurnberg, Germany
    Nucleic Acids Res 20:4193-8. 1992
    ..An approximately two-fold activation of the promoter by the UAS element was observed...
  66. ncbi Subunit exchange and the role of dimer flexibility in DNA binding by the Fis protein
    Stacy K Merickel
    Department of Biological Chemistry, School of Medicine, Howard Hughes Medical Institute, University of California, Los Angeles, Los Angeles, California 90095, USA
    Biochemistry 41:5788-98. 2002
    ..These results support a model in which the unusually short distance between DNA recognition helices on Fis is accommodated primarily through bending of the DNA...
  67. pmc Identification and characterization of the fis operon in enteric bacteria
    M B Beach
    Department of Biological Sciences, University at Albany, Albany, New York 12222, USA
    J Bacteriol 180:5932-46. 1998
    ..However, their promoter strengths vary, as do their responses to Fis autoregulation and integration host factor stimulation...
  68. pmc The Escherichia coli FIS protein is not required for the activation of tyrT transcription on entry into exponential growth
    L R Lazarus
    MRC Laboratory of Molecular Biology, Cambridge, UK
    EMBO J 12:2483-94. 1993
    ..In principle such a mechanism could confer a significant selective advantage thus accounting for the conservation of FIS binding sites in the regulatory regions of stable RNA promoters...
  69. pmc E.coli Fis protein activates ribosomal RNA transcription in vitro and in vivo
    W Ross
    Department of Bacteriology, University of Wisconsin, Madison 53706
    EMBO J 9:3733-42. 1990
    ..On the basis of these results, we propose roles for Fis and the upstream activation system in rRNA synthesis...
  70. pmc Growth phase-dependent regulation and stringent control of fis are conserved processes in enteric bacteria and involve a single promoter (fis P) in Escherichia coli
    Prabhat Mallik
    Department of Biological Sciences, University at Albany, SUNY, Albany, New York 12222, USA
    J Bacteriol 186:122-35. 2004
    ..We further show how certain conditions used in the primer extension reactions can generate artifacts resulting from secondary annealing events that are the likely source of incorrect assignment of additional fis promoters...
  71. pmc Biochemical identification of base and phosphate contacts between Fis and a high-affinity DNA binding site
    Yongping Shao
    Department of Biological Sciences, University at Albany, 1400 Washington Avenue, Albany, NY 12222, USA
    J Mol Biol 380:327-39. 2008
    ....
  72. ncbi Competition between NarL-dependent activation and Fis-dependent repression controls expression from the Escherichia coli yeaR and ogt promoters
    Derrick J P Squire
    University of Birmingham, Edgbaston, UK
    Biochem J 420:249-57. 2009
    ..The ogt gene encodes an O6-alkylguanine DNA alkyltransferase and, hence, this is the first report of expression of a DNA repair function being controlled by nitrate ions...
  73. pmc Location, degree, and direction of DNA bending associated with the Hin recombinational enhancer sequence and Fis-enhancer complex
    D Perkins-Balding
    Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, Georgia 30322, USA
    J Bacteriol 179:4747-53. 1997
    ..The positions and directions of DNA bends associated with the Fis-recombinational complex support a direct role for Fis-induced DNA bending in assembly of the active invertasome...
  74. ncbi FIS modulates growth phase-dependent topological transitions of DNA in Escherichia coli
    R Schneider
    Institut fur Genetik und Mikrobiologie, LMU Munchen, Germany
    Mol Microbiol 26:519-30. 1997
    ..Our results suggest that the primary role of FIS is to modulate chromosomal dynamics during bacterial growth...
  75. ncbi Transcription factor as a topological homeostat
    Georgi Muskhelishvili
    Max Planck Institute for Terrestrial Microbiology, Karl von Frisch Strasse, D 35043, Marburg, Germany
    Front Biosci 8:d279-85. 2003
    ..We argue that the coordinated effects of FIS on the global and local DNA architecture optimise gene expression by channelling the free energy of negative supercoiling to specific, biologically relevant sites...
  76. ncbi Architecture of Fis-activated transcription complexes at the Escherichia coli rrnB P1 and rrnE P1 promoters
    Sarah E Aiyar
    Department of Bacteriology, University of Wisconsin, 1550 Linden Drive, Madison, WI 53706 1567, USA
    J Mol Biol 316:501-16. 2002
    ..Thus, similar Fis-alphaCTD interactions are used for activation of transcription when the activator is bound at very different positions on the DNA...
  77. ncbi The transcriptional activator protein FIS: DNA interactions and cooperative interactions with RNA polymerase at the Escherichia coli rrnB P1 promoter
    A J Bokal
    Department of Bacteriology, University of Wisconsin Madison 53706
    J Mol Biol 245:197-207. 1995
    ....
  78. ncbi FIS-dependent trans-activation of tRNA and rRNA operons of Escherichia coli
    L Bosch
    Department of Biochemistry, Leiden University, Gorlaeus Laboratories, The Netherlands
    Biochim Biophys Acta 1050:293-301. 1990
    ..This protein is known to stimulate the inversion of various DNA segments by binding to cis-acting recombinational enhancers and functions as a host factor for the bacteriophages Mu and Lambda...
  79. pmc Molecular flip-flops formed by overlapping Fis sites
    Paul N Hengen
    Intramural Research Support Program, SAIC, NCI Frederick, Frederick, MD 21702 1201, USA
    Nucleic Acids Res 31:6663-73. 2003
    ..Alternatively, the flip-flop could represent part of an on-off switch for replication. Because they can be used to create precise switched states, molecular flip-flops could be used as the basis of a novel molecular computer...
  80. ncbi Effects of the Escherichia coli DNA-binding protein H-NS on rRNA synthesis in vivo
    H Afflerbach
    Institut für Physikalische Biologie, Heinrich Heine Universitat Dusseldorf, Germany
    Mol Microbiol 28:641-53. 1998
    ..Determination of the cellular ppGpp concentrations revealed that H-NS does not mediate its function via alterations in the synthesis of the global effector ppGpp...
  81. pmc Identification of genes negatively regulated by Fis: Fis and RpoS comodulate growth-phase-dependent gene expression in Escherichia coli
    J Xu
    Department of Biological Chemistry, UCLA School of Medicine 90024
    J Bacteriol 177:938-47. 1995
    ..We conclude that Fis negatively regulates a diverse set of genes and that RpoS can function to both activate and inhibit the expression of specific genes...
  82. ncbi Antagonistic control of the Escherichia coli bgl promoter by FIS and CAP in vitro
    A Caramel
    Institut fur Genetik, der Universität zu Köln, Weyertal 121, D 50931 Köln, Germany
    Mol Microbiol 36:85-92. 2000
    ..These results suggest that FIS contributes to silencing of the wild-type bgl promoter in vivo, presumably in the early exponential phase when FIS is predominantly expressed...
  83. pmc Sequence, regulation, and functions of fis in Salmonella typhimurium
    R Osuna
    Department of Biological Chemistry, School of Medicine, University of California, Los Angeles 90024, USA
    J Bacteriol 177:2021-32. 1995
    ..During the course of these studies, the sigma 28-dependent promoter within the hin-invertible segment that is responsible for fljB (H2) flagellin synthesis was precisely located...
  84. pmc Involvement of Fis protein in replication of the Escherichia coli chromosome
    M Filutowicz
    Department of Bacteriology, University of Wisconsin Madison 53706
    J Bacteriol 174:398-407. 1992
    ..Finally, purified Fis protein interacts in vitro with sites in oriC. These interactions could be involved in mediating the effect of Fis on DNA synthesis in vivo...
  85. ncbi Activation of transcription initiation from a stable RNA promoter by a Fis protein-mediated DNA structural transmission mechanism
    Michael L Opel
    Department of Microbiology and Molecular Genetics, College of Medicine, University of California, Irvine, CA 92697, USA
    Mol Microbiol 53:665-74. 2004
    ....
  86. ncbi FIS activates glnAp2 in Escherichia coli: role of a DNA bend centered at -55, upstream of the transcription start site
    Yi Xin Huo
    National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Peking University, Beijing, China
    FEMS Microbiol Lett 257:99-105. 2006
    ..In vitro transcription assays indicated that all DNA bends centered at -55 activate transcriptional initiation of glnAp2, especially when linear templates were used...
  87. pmc A role for the umuDC gene products of Escherichia coli in increasing resistance to DNA damage in stationary phase by inhibiting the transition to exponential growth
    S Murli
    Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    J Bacteriol 182:1127-35. 2000
    ..Thus, the umuDC gene products appear to increase DNA damage tolerance at least partially by regulating growth after DNA damage in both exponentially growing and stationary-phase cells...
  88. ncbi Multiple cis-acting sites positively regulate Escherichia coli nrd expression
    B A Jacobson
    Department of Biochemistry, University of Minnesota, St Paul 55108, USA
    Mol Microbiol 28:1315-22. 1998
    ....
  89. ncbi Potential binding sites of the trans-activator FIS are present upstream of all rRNA operons and of many but not all tRNA operons
    H Verbeek
    Department of Biochemistry, Leiden University, Gorleaus Laboratories, The Netherlands
    Biochim Biophys Acta 1050:302-6. 1990
    ..Accordingly, FIS binding to an upstream sequence of the metY(nusA) operon was found to be weaker than that to the UAS of the thrU(tufB) operon. No FIS binding sites were found upstream three tRNA operons...
  90. pmc Coactivation of the RpoS-dependent proP P2 promoter by fis and cyclic AMP receptor protein
    S M McLeod
    Department of Biological Chemistry, UCLA School of Medicine, Los Angeles, California 90095 1737, USA
    J Bacteriol 182:4180-7. 2000
    ..Efficient transcription in vitro requires that both activators must be preincubated with the DNA prior to addition of RNA polymerase...
  91. ncbi The expression of the Escherichia coli fis gene is strongly dependent on the superhelical density of DNA
    R Schneider
    Institut fur Genetik und Mikrobiologie, LMU Munchen, Maria Ward Str 1a, 80638 Munchen, Germany
    Mol Microbiol 38:167-75. 2000
    ..This strict dependence of fis expression on the superhelical density suggests that fis may be involved in 'fine-tuning' the homeostatic control mechanism of DNA supercoiling in E. coli...
  92. pmc Regulation of rRNA transcription correlates with nucleoside triphosphate sensing
    M M Barker
    Department of Bacteriology, University of Wisconsin, Madison, Wisconsin 53706, USA
    J Bacteriol 183:6315-23. 2001
    ....
  93. pmc Escherichia coli Fis and DnaA proteins bind specifically to the nrd promoter region and affect expression of an nrd-lac fusion
    L B Augustin
    Department of Biochemistry, University of Minnesota, St Paul 55108
    J Bacteriol 176:378-87. 1994
    ..Assay of in vivo expression from wild-type and DnaA box-mutated nrd promoter fragments fused to lacZ on single-copy plasmids indicates a positive effect of DnaA binding on expression of nrd...
  94. ncbi Novel growth rate control of dam gene expression in Escherichia coli
    L J Rasmussen
    Department of Pharmacology, University of Massachusetts Medical School, Worcester 01655
    Mol Microbiol 12:631-8. 1994
    ..The cde-4::miniTn10 insertion is located close to kilobase 670 on the physical map in or near the lipB gene...
  95. ncbi Analysis of the Fis-dependent and Fis-independent transcription activation mechanisms of the Escherichia coli ribosomal RNA P1 promoter
    M Zacharias
    Max Planck Institut fur Molekulare Genetik, Abteilung Wittmann, Berlin, Germany
    Biochemistry 31:2621-8. 1992
    ..In addition, the study demonstrates that both the helical geometry and the absolute distance of the UAS region relative to the promoter are crucial for transcription activation...
  96. ncbi Expression of the gene encoding the major bacterial nucleotide protein H-NS is subject to transcriptional auto-repression
    M Falconi
    Department of Biology, University of Camerino, Italy
    Mol Microbiol 10:273-82. 1993
    ..Binding of H-NS to this site is probably the ultimate cause of transcriptional auto-repression...
  97. ncbi FIS is a regulator of metabolism in Escherichia coli
    G Gonzalez-Gil
    Institut für Genbiologische Forschung Berlin GmbH, Berlin, Germany
    Mol Microbiol 22:21-9. 1996
    ..We conclude that FIS is a transcriptional modulator involved in the regulation of metabolism in E. coli...
  98. pmc Escherichia coli ribosome-associated protein SRA, whose copy number increases during stationary phase
    K Izutsu
    Institute for Virus Research, Kyoto University, Kyoto 606 8507, Japan
    J Bacteriol 183:2765-73. 2001
    ....
  99. ncbi Effects of FIS protein on rnpB transcription in Escherichia coli
    Hyun Sook Choi
    Department of Chemistry and Center for Molecular Design and Synthesis, Korea Advanced Institute of Science and Technology, Daejeon, Korea
    Mol Cells 19:239-45. 2005
    ..rnpB transcription was higher in the fis- than the fis+ cells during the transitions from lag to exponential phase, and from exponential to stationary phase...
  100. pmc Isolation of the gene encoding the Hin recombinational enhancer binding protein
    R C Johnson
    Department of Biological Chemistry, School of Medicine, University of California, Los Angeles 90024
    Proc Natl Acad Sci U S A 85:3484-8. 1988
    ..coli that lack a functional fis gene demonstrates that Fis is not essential for cell growth under laboratory conditions but is required for high rates of Hin-mediated site-specific inversion in vivo...
  101. ncbi Effects of Fis on ribosome synthesis and activity and on rRNA promoter activities in Escherichia coli
    X Zhang
    Molecular Program, University of Texas at Dallas, Richardson 75083 0688, USA
    J Mol Biol 259:27-40. 1996
    ....