escherichia coli proteins

Summary

Summary: Proteins obtained from ESCHERICHIA COLI.

Top Publications

  1. pmc WebLogo: a sequence logo generator
    Gavin E Crooks
    Department of Plant and Microbial Biology, University of California, Berkeley, California 94720, USA
    Genome Res 14:1188-90. 2004
  2. pmc Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter
    L M Guzman
    Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Bacteriol 177:4121-30. 1995
  3. pmc Quantifying E. coli proteome and transcriptome with single-molecule sensitivity in single cells
    Yuichi Taniguchi
    Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA
    Science 329:533-8. 2010
  4. pmc EcoCyc: a comprehensive database of Escherichia coli biology
    Ingrid M Keseler
    SRI International, 333 Ravenswood Ave, Menlo Park, CA 94025, USA
    Nucleic Acids Res 39:D583-90. 2011
  5. ncbi A common mechanism of cellular death induced by bactericidal antibiotics
    Michael A Kohanski
    Center for BioDynamics and Center for Advanced Biotechnology, Boston University, Boston, MA 02215, USA
    Cell 130:797-810. 2007
  6. ncbi Bacterial persistence as a phenotypic switch
    Nathalie Q Balaban
    Laboratory of Living Matter and Center for Studies in Physics and Biology, Rockefeller University, 1230 York Avenue, New York, NY 10021, USA
    Science 305:1622-5. 2004
  7. ncbi Principles of c-di-GMP signalling in bacteria
    Regine Hengge
    Institut für Biologie Mikrobiologie, Freie Universitat Berlin, 14195 Berlin, Germany
    Nat Rev Microbiol 7:263-73. 2009
  8. ncbi Stochastic gene expression in a single cell
    Michael B Elowitz
    Laboratory of Cancer Biology, Center for Studies in Physics and Biology, Rockefeller University, New York, NY 10021, USA
    Science 297:1183-6. 2002
  9. pmc Automated design of synthetic ribosome binding sites to control protein expression
    Howard M Salis
    Department of Pharmaceutical Chemistry, University of California San Francisco, San Francisco, California, USA
    Nat Biotechnol 27:946-50. 2009
  10. ncbi Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research
    Masanari Kitagawa
    Graduate School of Biological Sciences, Nara Institute of Science and Technology, Takayama, Ikoma, Nara, Japan
    DNA Res 12:291-9. 2005

Detail Information

Publications304 found, 100 shown here

  1. pmc WebLogo: a sequence logo generator
    Gavin E Crooks
    Department of Plant and Microbial Biology, University of California, Berkeley, California 94720, USA
    Genome Res 14:1188-90. 2004
    ..A command line interface and the complete, open WebLogo source code are available for local installation and customization...
  2. pmc Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter
    L M Guzman
    Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Bacteriol 177:4121-30. 1995
    ..We have exploited the tight regulation of the PBAD promoter to study the phenotypes of null mutations of essential genes and explored the use of pBAD vectors as an expression system...
  3. pmc Quantifying E. coli proteome and transcriptome with single-molecule sensitivity in single cells
    Yuichi Taniguchi
    Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA
    Science 329:533-8. 2010
    ..At high expression levels, the distributions are dominated by extrinsic noise. We found that a single cell's protein and mRNA copy numbers for any given gene are uncorrelated...
  4. pmc EcoCyc: a comprehensive database of Escherichia coli biology
    Ingrid M Keseler
    SRI International, 333 Ravenswood Ave, Menlo Park, CA 94025, USA
    Nucleic Acids Res 39:D583-90. 2011
    ..The graphical representation of signal transduction pathways has been updated, and the cellular and regulatory overviews were enhanced with new functionality. A specialized undergraduate teaching resource using EcoCyc is being developed...
  5. ncbi A common mechanism of cellular death induced by bactericidal antibiotics
    Michael A Kohanski
    Center for BioDynamics and Center for Advanced Biotechnology, Boston University, Boston, MA 02215, USA
    Cell 130:797-810. 2007
    ..g., reca...
  6. ncbi Bacterial persistence as a phenotypic switch
    Nathalie Q Balaban
    Laboratory of Living Matter and Center for Studies in Physics and Biology, Rockefeller University, 1230 York Avenue, New York, NY 10021, USA
    Science 305:1622-5. 2004
    ..Inherent heterogeneity of bacterial populations may be important in adaptation to fluctuating environments and in the persistence of bacterial infections...
  7. ncbi Principles of c-di-GMP signalling in bacteria
    Regine Hengge
    Institut für Biologie Mikrobiologie, Freie Universitat Berlin, 14195 Berlin, Germany
    Nat Rev Microbiol 7:263-73. 2009
    ..This Review focuses on emerging principles of c-di-GMP signalling using selected systems in different bacteria as examples...
  8. ncbi Stochastic gene expression in a single cell
    Michael B Elowitz
    Laboratory of Cancer Biology, Center for Studies in Physics and Biology, Rockefeller University, New York, NY 10021, USA
    Science 297:1183-6. 2002
    ..These results establish a quantitative foundation for modeling noise in genetic networks and reveal how low intracellular copy numbers of molecules can fundamentally limit the precision of gene regulation...
  9. pmc Automated design of synthetic ribosome binding sites to control protein expression
    Howard M Salis
    Department of Pharmaceutical Chemistry, University of California San Francisco, San Francisco, California, USA
    Nat Biotechnol 27:946-50. 2009
    ..The proposed forward engineering approach should accelerate the construction and systematic optimization of large genetic systems...
  10. ncbi Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research
    Masanari Kitagawa
    Graduate School of Biological Sciences, Nara Institute of Science and Technology, Takayama, Ikoma, Nara, Japan
    DNA Res 12:291-9. 2005
    ....
  11. ncbi Selective translation of leaderless mRNAs by specialized ribosomes generated by MazF in Escherichia coli
    Oliver Vesper
    Max F Perutz Laboratories, Center for Molecular Biology, Department of Microbiology, Immunobiology and Genetics, University of Vienna, Dr Bohrgasse 9 4, 1030 Vienna, Austria
    Cell 147:147-57. 2011
    ..Thus, we have discovered a modified translation machinery that is generated in response to MazF induction and that probably serves for stress adaptation in Escherichia coli...
  12. pmc The seed region of a small RNA drives the controlled destruction of the target mRNA by the endoribonuclease RNase E
    Katarzyna J Bandyra
    Department of Biochemistry, University of Cambridge, Cambridge CB2 1GA, England, UK
    Mol Cell 47:943-53. 2012
    ..These results suggest that the chemical signature and pairing status of the sRNA seed region may help to both 'proofread' recognition and activate mRNA cleavage, as part of a dynamic process involving cooperation of RNA, Hfq and RNase E...
  13. ncbi The twin-arginine translocation (Tat) protein export pathway
    Tracy Palmer
    Division of Molecular Microbiology, College of Life Sciences, University of Dundee, Dundee DD1 5EH, UK
    Nat Rev Microbiol 10:483-96. 2012
    ..In this Review, we summarize recent advances in our understanding of how this remarkable machine operates...
  14. pmc A small RNA regulates the expression of genes involved in iron metabolism in Escherichia coli
    Eric Massé
    Laboratory of Molecular Biology, National Cancer Institute, Building 37, Room 5132, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 99:4620-5. 2002
    ....
  15. ncbi A comprehensive library of fluorescent transcriptional reporters for Escherichia coli
    Alon Zaslaver
    Department of Molecular Cell Biology, Weizmann Institute of Science, 76100, Israel
    Nat Methods 3:623-8. 2006
    ..coli, including putative internal promoters within previously known operons, such as the lac operon. This library can serve as a tool for accurate, high-resolution analysis of transcription networks in living E. coli cells...
  16. pmc Bacterial persistence by RNA endonucleases
    Etienne Maisonneuve
    Centre for Bacterial Cell Biology, Institute for Cell and Molecular Biosciences, Newcastle University, Newcastle upon Tyne NE2 4AX, United Kingdom
    Proc Natl Acad Sci U S A 108:13206-11. 2011
    ..Many pathogenic bacteria known to enter dormant states have a plethora of TA genes. Therefore, in the future, the discoveries described here may lead to a mechanistic understanding of the persistence phenomenon in pathogenic bacteria...
  17. pmc Cellular defenses against superoxide and hydrogen peroxide
    James A Imlay
    Department of Microbiology, University of Illinois, Urbana, IL 61801, USA
    Annu Rev Biochem 77:755-76. 2008
    ..Current research seeks to identify these, and bacteria comprise an exceptionally accessible experimental system that has provided many of the answers. This manuscript reviews recent developments and identifies remaining puzzles...
  18. pmc Absolute metabolite concentrations and implied enzyme active site occupancy in Escherichia coli
    Bryson D Bennett
    Department of Chemistry and Lewis Sigler Institute for Integrative Genomics, Princeton University, Princeton, New Jersey, USA
    Nat Chem Biol 5:593-9. 2009
    ..The data and analyses presented here highlight the ability to identify organizing metabolic principles from systems-level absolute metabolite concentration data...
  19. pmc Ciprofloxacin causes persister formation by inducing the TisB toxin in Escherichia coli
    Tobias Dörr
    Department of Biology, Northeastern University, Antimicrobial Discovery Center, Boston, Massachusetts, United States of America
    PLoS Biol 8:e1000317. 2010
    ..These results suggest that a DNA damage-induced toxin controls production of multidrug tolerant cells and thus provide a model of persister formation...
  20. pmc Rate and molecular spectrum of spontaneous mutations in the bacterium Escherichia coli as determined by whole-genome sequencing
    Heewook Lee
    School of Informatics and Computing, Indiana University, Bloomington, IN 47405, USA
    Proc Natl Acad Sci U S A 109:E2774-83. 2012
    ....
  21. ncbi Multidrug-resistance efflux pumps - not just for resistance
    Laura J V Piddock
    Antimicrobial Agents Research Group, Division of Immunity and Infection, The Medical School, University of Birmingham, Birmingham B15 2TT, UK
    Nat Rev Microbiol 4:629-36. 2006
    ..Here, I present the accumulating evidence that multidrug-resistance efflux pumps have roles in bacterial pathogenicity and propose that these pumps therefore have greater clinical relevance than is usually attributed to them...
  22. ncbi Mechanisms of cyclic-di-GMP signaling in bacteria
    Urs Jenal
    Biozentrum of the University of Basel, CH 4056 Basel, Switzerland
    Annu Rev Genet 40:385-407. 2006
    ..In addition, we examine several well-established model systems for c-di-GMP signaling, including Pseudomonas, Vibrio, Caulobacter, and Salmonella...
  23. ncbi Protein translocation across the bacterial cytoplasmic membrane
    Arnold J M Driessen
    Department of Molecular Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute and the Zernike Institute for Advanced Materials, University of Groningen, Haren, The Netherlands
    Annu Rev Biochem 77:643-67. 2008
    ..This review summarizes the present knowledge of the mechanism and structure of the Sec translocase, with a special emphasis on unresolved questions and topics of current research...
  24. pmc Growth phase-dependent variation in protein composition of the Escherichia coli nucleoid
    T Ali Azam
    Department of Molecular Genetics, National Institute of Genetics, Mishima, Shizuoka 411 8540, Japan
    J Bacteriol 181:6361-70. 1999
    ..These changes in the composition of nucleoid-associated proteins in the stationary phase are accompanied by compaction of the genome DNA and silencing of the genome functions...
  25. ncbi DNA mismatch repair
    Thomas A Kunkel
    Laboratory of Molecular Genetics and Laboratory of Structural Biology, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709, USA
    Annu Rev Biochem 74:681-710. 2005
    ..Emphasis is on structure-function studies of MMR proteins, on how mismatches are recognized, on the process by which the newly replicated strand is identified, and on excision of the replication error...
  26. pmc Specialized persister cells and the mechanism of multidrug tolerance in Escherichia coli
    Iris Keren
    Department of Biology, Northeastern University, 134 Mugar Hall, 360 Huntington Ave, Boston, MA 02115
    J Bacteriol 186:8172-80. 2004
    ..The function of these specialized dormant cells is to ensure the survival of the population in the presence of lethal factors...
  27. ncbi Femtomolar sensitivity of metalloregulatory proteins controlling zinc homeostasis
    C E Outten
    Department of Chemistry, Northwestern University, 2145 Sheridan Road, Evanston, IL 60208, USA
    Science 292:2488-92. 2001
    ..Thus, cells exert tight control over cytosolic metal concentrations, even for relatively low-toxicity metals such as zinc...
  28. pmc A small RNA that regulates motility and biofilm formation in response to changes in nutrient availability in Escherichia coli
    Maureen K Thomason
    Cell Biology and Metabolism Program, Eunice Kennedy Shriver National Institute of Child Health and Human Development, Bethesda, MD, USA
    Mol Microbiol 84:17-35. 2012
    ..Based on our observations, we propose that, in response to limited nutrient availability, increasing levels of McaS modulate steps in the progression to a sessile lifestyle...
  29. ncbi Effects of nucleoid-associated proteins on bacterial chromosome structure and gene expression
    Douglas F Browning
    School of Immunity and Infection, University of Birmingham, Birmingham B15 2TT, UK
    Curr Opin Microbiol 13:773-80. 2010
    ..Some specific examples, involving the E. coli IHF and Fis proteins, that illustrate new principles, are described in detail...
  30. pmc Probing transcription factor dynamics at the single-molecule level in a living cell
    Johan Elf
    Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA
    Science 316:1191-4. 2007
    ..The methods and findings can be generalized to other nucleic acid binding proteins...
  31. pmc Colicin biology
    Eric Cascales
    Laboratoire d Ingénierie des Systèmes Macromoléculaires, Institut de Biologie Structurale et Microbiologie, Centre National de la Recherche Scientifique, UPR 9027, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
    Microbiol Mol Biol Rev 71:158-229. 2007
    ..The mechanisms of inhibition by specific and cognate immunity proteins are presented. Finally, the use of colicins as laboratory or biotechnological tools and their mode of evolution are discussed...
  32. pmc Structure of Escherichia coli Hfq bound to polyriboadenylate RNA
    Todd M Link
    Department of Biochemistry and Molecular Biology, University of Texas, MD Anderson Cancer Center, Houston, TX 77030 4009, USA
    Proc Natl Acad Sci U S A 106:19292-7. 2009
    ..The abundance of (A-R-N)(4) and (A-R-N)(5) triplet repeats in the E. coli genome suggests additional RNA targets for Hfq. Further, the structure provides insight into Hfq-mediated sRNA-mRNA annealing and the role of Hfq in RNA decay...
  33. pmc In vivo architecture and action of bacterial structural maintenance of chromosome proteins
    Anjana Badrinarayanan
    Department of Biochemistry, University of Oxford, UK
    Science 338:528-31. 2012
    ..Thus, by functioning in pairs, MukBEF complexes may undergo multiple cycles of ATP hydrolysis without being released from DNA, analogous to the behavior of well-characterized molecular motors...
  34. pmc Structure and function of the feed-forward loop network motif
    S Mangan
    Departments of Molecular Cell Biology and Physics of Complex Systems, Weizmann Institute of Science, Rehovot 76100, Israel
    Proc Natl Acad Sci U S A 100:11980-5. 2003
    ..Additional features, such as pulse generation and cooperativity, are discussed. This study defines the function of one of the most significant recurring circuit elements in transcription networks...
  35. ncbi Biosynthesis and assembly of capsular polysaccharides in Escherichia coli
    Chris Whitfield
    Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario N1G 2W1, Canada
    Annu Rev Biochem 75:39-68. 2006
    ..These systems have an impact on both a general understanding of membrane trafficking in bacteria and on bacterial pathogenesis...
  36. pmc SSB as an organizer/mobilizer of genome maintenance complexes
    Robert D Shereda
    Department of Biomolecular Chemistry, University of Wisconsin School of Medicine and Public Health, Madison, WI, USA
    Crit Rev Biochem Mol Biol 43:289-318. 2008
    ..Similar themes to those highlighted in this review are evident in all biological systems...
  37. pmc Analysis of Pseudomonas aeruginosa diguanylate cyclases and phosphodiesterases reveals a role for bis-(3'-5')-cyclic-GMP in virulence
    Hemantha Kulasakara
    Department of Microbiology and Molecular Genetics, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 103:2839-44. 2006
    ....
  38. ncbi Interdependence of cell growth and gene expression: origins and consequences
    Matthew Scott
    Center for Theoretical Biological Physics, Department of Physics, University of California, San Diego, La Jolla, CA 92093, USA
    Science 330:1099-102. 2010
    ..The use of such empirical relations, analogous to phenomenological laws, may facilitate our understanding and manipulation of complex biological systems before underlying regulatory circuits are elucidated...
  39. pmc Coupled degradation of a small regulatory RNA and its mRNA targets in Escherichia coli
    Eric Massé
    Laboratory of Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Genes Dev 17:2374-83. 2003
    ..Thus, this large class of regulatory RNAs share an unexpected intrinsic mechanism for shutting off their action...
  40. pmc Dissemination of clonally related Escherichia coli strains expressing extended-spectrum beta-lactamase CTX-M-15
    Teresa M Coque
    Hospital Universitario Ramon y Cajal, Madrid, Spain
    Emerg Infect Dis 14:195-200. 2008
    ..Most plasmids contained blaOXA-1, aac(6')-Ib-cr, and blaTEM-1. Worldwide dissemination of CTX-M-15 seems to be determined by clonal complexes ST131 and ST405 and multidrug-resistant IncFII plasmids...
  41. pmc Replication fork stalling at natural impediments
    Ekaterina V Mirkin
    Department of Biology, Tufts University, Medford, MA 02155, USA
    Microbiol Mol Biol Rev 71:13-35. 2007
    ..This review describes the mechanisms and consequences of replication stalling at various natural impediments, with an emphasis on the role of replication stalling in genomic instability...
  42. pmc An interdomain energetic tug-of-war creates the allosterically active state in Hsp70 molecular chaperones
    Anastasia Zhuravleva
    Department of Biochemistry and Molecular Biology, University of Massachusetts, Amherst, MA 01003, USA
    Cell 151:1296-307. 2012
    ..The resulting energetic tension underlies Hsp70 functional properties and enables them to be modulated by ligands and cochaperones and "tuned" through evolution...
  43. pmc Structural insights into the dynamics and function of the C-terminus of the E. coli RNA chaperone Hfq
    Mads Beich-Frandsen
    Department of Structural and Computational Biology, Max F Perutz Laboratories, University of Vienna, Campus Vienna Biocenter 5, A 1030 Vienna, Austria
    Nucleic Acids Res 39:4900-15. 2011
    ..Furthermore, SRCD spectroscopy supported the hypothesis that RNA fragments exceeding a certain length interact with the C-termini of Hfq(Ec)...
  44. pmc Hexamer to monomer equilibrium of E. coli Hfq in solution and its impact on RNA annealing
    Subrata Panja
    T C Jenkins Department of Biophysics, Johns Hopkins University, 3400 North Charles Street, Baltimore, MD 21218, USA
    J Mol Biol 417:406-12. 2012
    ..These results suggest that RNA binding is coupled to hexamer assembly and that the biochemical activity of Hfq reflects the equilibrium between different quaternary structures...
  45. pmc Structures of the bacterial ribosome in classical and hybrid states of tRNA binding
    Jack A Dunkle
    Department of Molecular and Cell Biology, University of California at Berkeley, Berkeley, CA 94720, USA
    Science 332:981-4. 2011
    ..The structures help to explain how the ratchet-like motion of the two ribosomal subunits contributes to the mechanisms of translocation, termination, and ribosome recycling...
  46. ncbi MipZ, a spatial regulator coordinating chromosome segregation with cell division in Caulobacter
    Martin Thanbichler
    Department of Developmental Biology, Stanford University School of Medicine, Beckman Center B300, 279 Campus Drive, Stanford, CA 94305, USA
    Cell 126:147-62. 2006
    ..The cellular localization of MipZ thus serves the dual function of positioning the FtsZ ring and delaying formation of the cell division apparatus until chromosome segregation has initiated...
  47. ncbi Escherichia coli sequence type ST131 as the major cause of serious multidrug-resistant E. coli infections in the United States
    James R Johnson
    Infectious Diseases, VA Medical Center, Minneapolis, MN 55417, USA
    Clin Infect Dis 51:286-94. 2010
    ..Escherichia coli sequence type ST131 (O25:H4), associated with the CTX-M-15 extended-spectrum beta-lactamase, has emerged internationally as a multidrug-resistant pathogen but has received little attention in the United States...
  48. pmc Evidence for an autonomous 5' target recognition domain in an Hfq-associated small RNA
    Kai Papenfort
    Institute for Molecular Infection Biology, University of Wurzburg, D 97080 Wurzburg, Germany
    Proc Natl Acad Sci U S A 107:20435-40. 2010
    ..Autonomous 5' domains seem more common in sRNAs than appreciated and might improve the design of synthetic RNA regulators...
  49. ncbi Global analysis of small RNA and mRNA targets of Hfq
    Aixia Zhang
    Cell Biology and Metabolism Branch, National Institute of Child Health and Development, Bethesda MD 20892, USA
    Mol Microbiol 50:1111-24. 2003
    ..Thus Hfq immunoprecipitation followed by microarray analysis is a highly effective method for detecting a major class of small RNAs as well as identifying new Hfq functions...
  50. ncbi A synthetic oscillatory network of transcriptional regulators
    M B Elowitz
    Department of Molecular Biology and Physics, Princeton University, New Jersey 08544, USA
    Nature 403:335-8. 2000
    ..Such 'rational network design may lead both to the engineering of new cellular behaviours and to an improved understanding of naturally occurring networks...
  51. pmc Inverse regulatory coordination of motility and curli-mediated adhesion in Escherichia coli
    Christina Pesavento
    Institut für Biologie Mikrobiologie, Freie Universitat Berlin, 14195 Berlin, Germany
    Genes Dev 22:2434-46. 2008
    ..In summary, these data reveal the logic and sequence of molecular events underlying the motile-to-adhesive "lifestyle" switch in E. coli...
  52. ncbi Advanced genetic strategies for recombinant protein expression in Escherichia coli
    Hans Peter Sørensen
    Laboratory of BioDesign, Department of Molecular Biology, Aarhus University, Gustav Wieds Vej 10 C, DK 8000 Aarhus C, Denmark
    J Biotechnol 115:113-28. 2005
    ..The macromolecular background for a variety of obstacles and genetic state-of-the-art solutions are presented...
  53. ncbi The RNA degradosome of Escherichia coli: an mRNA-degrading machine assembled on RNase E
    Agamemnon J Carpousis
    Laboratoire de Microbiologie et Genetique Moleculaires, Unité Mixte de Recherche 5100, Centre National de la Recherche Scientifique et Université Paul Sabatier, 31062 Toulouse, France
    Annu Rev Microbiol 61:71-87. 2007
    ....
  54. pmc Bacterial toxin-antitoxin systems: more than selfish entities?
    Laurence Van Melderen
    Laboratoire de Génétique et Physiologie Bactérienne, IBMM, Faculte des Sciences, Universite Libre de Bruxelles, Gosselies, Belgium
    PLoS Genet 5:e1000437. 2009
    ..This Review discusses current hypotheses regarding the biological roles of these evolutionarily successful small operons. We consider the various selective forces that could drive the maintenance of TA systems in bacterial genomes...
  55. ncbi Positive regulation of motility and flhDC expression by the RNA-binding protein CsrA of Escherichia coli
    B L Wei
    Department of Molecular Biology and Immunology, University of North Texas Health Science Center at Fort Worth, 3500 Camp Bowie Blvd, Fort Worth, TX 76107 2699, USA
    Mol Microbiol 40:245-56. 2001
    ..Thus, CsrA stimulates flhDC gene expression by a post-transcriptional mechanism reminiscent of its function in the repression of glycogen biosynthesis...
  56. pmc Stoichiometry and architecture of active DNA replication machinery in Escherichia coli
    Rodrigo Reyes-Lamothe
    Department of Biochemistry, University of Oxford, Oxford OX1 3QU, UK
    Science 328:498-501. 2010
    ..Single-strand binding protein has a broader spatial distribution than the core components, with 5 to 11 tetramers per replisome. This in vivo technique could provide single-molecule insight into other molecular machines...
  57. pmc Analysis of a complete library of putative drug transporter genes in Escherichia coli
    K Nishino
    Department of Cell Membrane Biology, Institute of Scientific and Industrial Research, Osaka University, Ibaraki, Osaka 567-0047, Japan
    J Bacteriol 183:5803-12. 2001
    ..Of these 20 ORFs, cusA, yegMNO, ydgFE, yceE, yceL, yidY, and ybjYZ are novel drug resistance genes. The fsr, bcr, yjiO, ydhE, acrD, and yhiUV genes gave broader resistance spectra than previously reported...
  58. pmc Chromosome organization by a nucleoid-associated protein in live bacteria
    Wenqin Wang
    Department of Physics, Harvard University, Cambridge, MA 02138, USA
    Science 333:1445-9. 2011
    ..Deleting H-NS led to substantial chromosome reorganization. These observations demonstrate that H-NS plays a key role in global chromosome organization in bacteria...
  59. pmc RNAs actively cycle on the Sm-like protein Hfq
    Aurélie Fender
    Department of Cell and Molecular Biology, Biomedical Center, Uppsala University, Sweden
    Genes Dev 24:2621-6. 2010
    ..This solves the "strong binding-high turnover" paradox and permits efficient use of the Hfq pool...
  60. pmc Cryo-EM structure of the ribosome-SecYE complex in the membrane environment
    Jens Frauenfeld
    Gene Center, Department for Biochemistry, University of Munich, Munich, Germany
    Nat Struct Mol Biol 18:614-21. 2011
    ..On the basis of our map and molecular dynamics simulations, we present a model of a signal anchor-gated PCC in the membrane...
  61. ncbi Hfq binding at RhlB-recognition region of RNase E is crucial for the rapid degradation of target mRNAs mediated by sRNAs in Escherichia coli
    Yoshiki Ikeda
    Division of Biological Science, Graduate School of Science, Nagoya University, Chikusa, Nagoya 464 8602, Japan
    Mol Microbiol 79:419-32. 2011
    ..Finally, we demonstrate that overexpression of RhlB partially inhibits the Hfq binding to RNase E and the rapid degradation of ptsG mRNA...
  62. ncbi The effects of alternate optimal solutions in constraint-based genome-scale metabolic models
    R Mahadevan
    Genomatica, Inc, Bioprocessing Division, 5405 Morehouse Drive, Suite 210, San Diego, CA 92121, USA
    Metab Eng 5:264-76. 2003
    ..These methods also provide an efficient and robust method to calculate the range of flux distributions that can be derived from quantitative fermentation data...
  63. ncbi Escherichia coli harboring Shiga toxin 2 gene variants: frequency and association with clinical symptoms
    Alexander W Friedrich
    Institut fur Hygiene und Mikrobiologie der Universitat Wurzburg, Wurzburg, Germany
    J Infect Dis 185:74-84. 2002
    ..stx2c-positive STEC isolates can cause HUS, but the presence of stx2d or stx2e may predict a milder disease with a minimal risk of HUS...
  64. ncbi Identification and characterization of E. coli CRISPR-cas promoters and their silencing by H-NS
    Umit Pul
    Institut für Physikalische Biologie, Heinrich Heine Universitat Dusseldorf, Dusseldorf, Germany
    Mol Microbiol 75:1495-512. 2010
    ..Our results demonstrate an active involvement of H-NS in the induction of the CRISPR-cas system and suggest a potential link between two prokaryotic defence systems against foreign DNA...
  65. ncbi The bacterial LexA transcriptional repressor
    M Butala
    School of Biosciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, United Kingdom
    Cell Mol Life Sci 66:82-93. 2009
    ..These may provide new starting points in the battle against the emergence of bacterial pathogens and the spread of drug resistance among them...
  66. pmc The stringent response and cell cycle arrest in Escherichia coli
    Daniel J Ferullo
    Department of Biology and Rosenstiel Basic Medical Sciences Research Center, Brandeis University, Waltham, MA, USA
    PLoS Genet 4:e1000300. 2008
    ..This is the first indication that bacterial chromosome segregation, whose mechanism is not understood, is a step that may be regulated in response to environmental conditions...
  67. ncbi Optimization of membrane protein overexpression and purification using GFP fusions
    David Drew
    Department of Biochemistry and Biophysics, Stockholm University, SE 106 91 Stockholm, Sweden
    Nat Methods 3:303-13. 2006
  68. ncbi Oxidative stress
    G Storz
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Curr Opin Microbiol 2:188-94. 1999
    ..These studies have also shown that the oxidative stress responses are intimately coupled to other regulatory networks in the cell...
  69. ncbi X-ray structure of a protein-conducting channel
    Bert van den Berg
    Howard Hughes Medical Institute and Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, Massachusetts 02115, USA
    Nature 427:36-44. 2004
    ....
  70. ncbi Disassembly of the Mu transposase tetramer by the ClpX chaperone
    I Levchenko
    Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge 02139, USA
    Genes Dev 9:2399-408. 1995
    ..These data contribute to the emerging picture that members of the Clp family are chaperones specifically suited for disaggregating proteins and are able to function with or without a collaborating protease...
  71. pmc Genome-wide analysis of Fis binding in Escherichia coli indicates a causative role for A-/AT-tracts
    Byung Kwan Cho
    Department of Bioengineering, University of California San Diego, La Jolla, California 92093 0412, USA
    Genome Res 18:900-10. 2008
    ..These novel results provide new insight into how Fis modulates DNA topology at a genome scale and thus advance our understanding of the architectural bases of the E. coli nucleoid...
  72. pmc Expression of flagella is coincident with uropathogenic Escherichia coli ascension to the upper urinary tract
    M Chelsea Lane
    Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI 48109 0620, USA
    Proc Natl Acad Sci U S A 104:16669-74. 2007
    ..These data provide compelling evidence that wild-type UPEC express flagellin and presumably utilize flagellum-mediated motility during UTI to ascend to the upper urinary tract and disseminate within the host...
  73. ncbi Proteome-wide analysis of chaperonin-dependent protein folding in Escherichia coli
    Michael J Kerner
    Department of Cellular Biochemistry, Max Planck Institute of Biochemistry, Am Klopferspitz 18, D 82152 Martinsried, Germany
    Cell 122:209-20. 2005
    ..We suggest that the chaperonin system may have facilitated the evolution of this fold into a versatile platform for the implementation of numerous enzymatic functions...
  74. ncbi Structure of a Na+/H+ antiporter and insights into mechanism of action and regulation by pH
    Carola Hunte
    Department of Molecular Membrane Biology, Max Planck Institute of Biophysics, Max von Laue Str 3, D 60438 Frankfurt, Germany
    Nature 435:1197-202. 2005
    ..This ion-exchange machinery is regulated by a conformational change elicited by a pH signal perceived at the entry to the cytoplasmic funnel...
  75. ncbi Crystal structures of a multidrug transporter reveal a functionally rotating mechanism
    Satoshi Murakami
    Department of Cell Membrane Biology, Institute of Scientific and Industrial Research, Osaka University, Ibaraki, Osaka 567 0047, Japan
    Nature 443:173-9. 2006
    ..The voluminous binding pocket is aromatic and allows multi-site binding. The structures indicate that drugs are exported by a three-step functionally rotating mechanism in which substrates undergo ordered binding change...
  76. ncbi MutT protein specifically hydrolyses a potent mutagenic substrate for DNA synthesis
    H Maki
    Department of Biochemistry, Faculty of Medicine, Kyushu University, Fukuoka, Japan
    Nature 355:273-5. 1992
    ..This indicates that elimination from the nucleotide pool of the oxidized form of guanine nucleotide is important for the high fidelity of DNA synthesis...
  77. pmc Targeting of csgD by the small regulatory RNA RprA links stationary phase, biofilm formation and cell envelope stress in Escherichia coli
    Franziska Mika
    Institut für Biologie Mikrobiologie, Freie Universitat Berlin, 14195 Berlin, Germany
    Mol Microbiol 84:51-65. 2012
    ..Thus, antagonistic regulation of csgD and RprA at the mRNA level integrates cell envelope stress signals with global gene expression during stationary phase and biofilm formation...
  78. pmc PolyU tail of rho-independent terminator of bacterial small RNAs is essential for Hfq action
    Hironori Otaka
    Division of Biological Science, Graduate School of Science, Nagoya University, Chikusa, Nagoya 464 8602, Japan
    Proc Natl Acad Sci U S A 108:13059-64. 2011
    ..We conclude that the polyU tail of sRNAs is essential for Hfq action in general. The data also indicate that the terminator polyU tail plays a role in Hfq-dependent stabilization of sRNAs...
  79. ncbi Optimality and evolutionary tuning of the expression level of a protein
    Erez Dekel
    Department of Molecular Cell Biology and Department of Physics of Complex Systems, The Weizmann Institute of Science, Rehovot 76100, Israel
    Nature 436:588-92. 2005
    ..Thus, protein expression from the lac operon seems to be a solution of a cost-benefit optimization problem, and can be rapidly tuned by evolution to function optimally in new environments...
  80. ncbi LEEways: tales of EPEC, ATEC and EHEC
    M Alexander Schmidt
    Institut für Infektiologie Zentrum für Molekularbiologie der Entzündung ZMBE, Westfalische Wilhelms Universitat Munster, Von Esmarch Str 56, D 48149 Munster, Germany
    Cell Microbiol 12:1544-52. 2010
    ..coli during infection...
  81. pmc A stochastic single-molecule event triggers phenotype switching of a bacterial cell
    Paul J Choi
    Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA
    Science 322:442-6. 2008
    ..Hence, a stochastic single-molecule event determines a cell's phenotype...
  82. pmc Cooperation of Escherichia coli Hfq hexamers in DsrA binding
    Weiwei Wang
    Hefei National Laboratory for Physical Sciences at Microscale, School of Life Sciences, University of Science and Technology of China, Hefei, Anhui 230026, China
    Genes Dev 25:2106-17. 2011
    ..Fluorescence resonance energy transfer conducted with full-length Hfq and DsrA also supports cooperation of Hfq hexamers upon DsrA binding. The implications of Hfq hexamer cooperation have been discussed...
  83. ncbi SOS response induction by beta-lactams and bacterial defense against antibiotic lethality
    Christine Miller
    Department of Genetics, Stanford University, Stanford, CA 94305, USA
    Science 305:1629-31. 2004
    ....
  84. pmc Sm-like proteins in Eubacteria: the crystal structure of the Hfq protein from Escherichia coli
    Claude Sauter
    European Molecular Biology Laboratory, Structural and Computational Biology Programme, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
    Nucleic Acids Res 31:4091-8. 2003
    ..Finally, a detailed structural comparison shows that the Sm-fold is remarkably well conserved in bacteria, Archaea and Eukarya, and represents a universal and modular building unit for oligomeric RNA binding proteins...
  85. ncbi Enteropathogenic and enterohaemorrhagic Escherichia coli: even more subversive elements
    Alexander R C Wong
    Centre for Molecular Microbiology and Infection, Division of Cell and Molecular Biology, Imperial College London, London SW7 2AZ, UK
    Mol Microbiol 80:1420-38. 2011
    ....
  86. ncbi Managing membrane stress: the phage shock protein (Psp) response, from molecular mechanisms to physiology
    Nicolas Joly
    Division of Biology, Imperial College London, South Kensington, London, UK
    FEMS Microbiol Rev 34:797-827. 2010
    ....
  87. pmc DNA microarray-mediated transcriptional profiling of the Escherichia coli response to hydrogen peroxide
    M Zheng
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    J Bacteriol 183:4562-70. 2001
    ..These results expand our understanding of the oxidative stress response and raise interesting questions regarding the nature of other regulators that modulate gene expression in response to hydrogen peroxide...
  88. pmc RNase E-based ribonucleoprotein complexes: mechanical basis of mRNA destabilization mediated by bacterial noncoding RNAs
    Teppei Morita
    Division of Biological Science, Graduate School of Science, Nagoya University, Chikusa, Nagoya 464 8602, Japan
    Genes Dev 19:2176-86. 2005
    ..The formation of ribonucleoprotein complexes containing RNases could be a general way by which small RNAs destabilize target mRNAs in both prokaryotes and eukaryotes...
  89. ncbi Membrane protein architects: the role of the BAM complex in outer membrane protein assembly
    Timothy J Knowles
    Cancer Research UK Institute for Cancer Studies, School of Cancer Sciences, Edgbaston, Birmingham, UK
    Nat Rev Microbiol 7:206-14. 2009
    ..Here, we discuss how these structures illuminate the mechanisms responsible for the biogenesis of outer membrane proteins...
  90. pmc Associations between virulence factors of Shiga toxin-producing Escherichia coli and disease in humans
    P Boerlin
    Department of Population Medicine, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada
    J Clin Microbiol 37:497-503. 1999
    ....
  91. pmc The C-terminal domain of Escherichia coli Hfq is required for regulation
    Branislav Vecerek
    Max F Perutz Laboratories, University of Vienna, Dr Bohrgasse 9, 1030 Vienna, Austria
    Nucleic Acids Res 36:133-43. 2008
    ..These studies indicate that the C-terminal extension of E. coli Hfq constitutes a hitherto unrecognized RNA interaction surface with specificity for mRNAs...
  92. pmc The stoichiometry of the Escherichia coli Hfq protein bound to RNA
    Taylor B Updegrove
    School of Biology, Georgia Institute of Technology, Atlanta, Georgia 30332, USA
    RNA 17:489-500. 2011
    ....
  93. ncbi CsrA post-transcriptionally represses pgaABCD, responsible for synthesis of a biofilm polysaccharide adhesin of Escherichia coli
    Xin Wang
    Department of Microbiology and Immunology, Emory University School of Medicine, 3105 Rollins Research Center, 1510 Clifton Road N E, Atlanta, GA 30322, USA
    Mol Microbiol 56:1648-63. 2005
    ..These studies define the crucial mechanisms, though not the only means, by which the Csr system influences biofilm formation...
  94. pmc The Pic protease of enteroaggregative Escherichia coli promotes intestinal colonization and growth in the presence of mucin
    Susan M Harrington
    Center for Vaccine Development, University of Maryland, Baltimore, MD 21201, USA
    Infect Immun 77:2465-73. 2009
    ..The data suggest a novel metabolic role for the Pic mucinase in EAEC colonization...
  95. pmc YeeV is an Escherichia coli toxin that inhibits cell division by targeting the cytoskeleton proteins, FtsZ and MreB
    Qian Tan
    Department of Biochemistry, Center for Advanced Biotechnology and Medicine, Robert Wood Johnson Medical School, 679 Hoes Lane, Piscataway, NJ 08854, USA
    Mol Microbiol 79:109-18. 2011
    ..The YeeV toxin is distinct from other well-studied toxins: it directs the binding of two cytoskeletal proteins and inhibits FtsZ and MreB simultaneously...
  96. pmc Direct and indirect effects of H-NS and Fis on global gene expression control in Escherichia coli
    Christina Kahramanoglou
    Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QP, UK
    Nucleic Acids Res 39:2073-91. 2011
    ..Our study serves as a proof-of-principle for the use of ChIP-seq for global DNA-binding proteins in bacteria, which should become significantly more economical and feasible with the development of multiplexing techniques...
  97. ncbi Despite similar binding to the Hfq protein regulatory RNAs widely differ in their competition performance
    Mikołaj Olejniczak
    Institute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12 14, Poznań, Poland
    Biochemistry 50:4427-40. 2011
    ..Overall, the results suggest that despite the uniform binding of different sRNAs to the same site on Hfq their exchange on this protein is dependent on the identities of the competing sRNAs...
  98. pmc Preserving the membrane barrier for small molecules during bacterial protein translocation
    Eunyong Park
    Howard Hughes Medical Institute and Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, Massachusetts 02115, USA
    Nature 473:239-42. 2011
    ..The structural conservation of the channel in all organisms indicates that this may be a universal mechanism by which the membrane barrier is maintained during protein translocation...
  99. ncbi Competition among Hfq-binding small RNAs in Escherichia coli
    Kyung Moon
    Laboratory of Molecular Biology, National Cancer Institute, Bethesda, MD 20892, USA
    Mol Microbiol 82:1545-62. 2011
    ..These results support the existence of a hierarchy of sRNA competition for Hfq, modulating the function of some sRNAs...
  100. ncbi Structural basis for recognition and repair of the endogenous mutagen 8-oxoguanine in DNA
    S D Bruner
    Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts 02138, USA
    Nature 403:859-66. 2000
    ..One known mutation, R154H, converts hOGG1 to a promutator by relaxing the specificity of the enzyme for the base opposite oxoG...
  101. pmc Structure of the SSB-DNA polymerase III interface and its role in DNA replication
    Aimee H Marceau
    Department of Biomolecular Chemistry, University of Wisconsin School of Medicine and Public Health, Madison, WI 53706 1532, USA
    EMBO J 30:4236-47. 2011
    ..Destabilization of the χ/SSB complex in vivo produces cells with temperature-dependent cell cycle defects that appear to arise from replisome instability...

Research Grants64

  1. Single-molecule visualization of transcription regulation mechanisms
    Jeff Gelles; Fiscal Year: 2013
    ..processes involved in regulation of transcription initiation and elongation in vitro using purified Escherichia coli proteins in reconstituted transcription reactions...
  2. MOLECULAR ANALYSIS OF FERRIC ENTEROBACTIN TRANSPORT
    MARK MCINTOSH; Fiscal Year: 1993
    ..particular, this proposal is designed to delineate structural and functional features of three specific Escherichia coli proteins involved in the biosynthesis (EntD) of the catechol siderophore enterobactin (enterochelin) and the ..
  3. Structural Insights Into Regulation of SOS Mutagenesis
    Martin Gonzalez; Fiscal Year: 2003
    ..An example of these are the Escherichia coli proteins UmuD' (UmuD) and UmuC...
  4. Protein Transport Across Cell Membranes Gordon Conference 2005
    Ross Dalbey; Fiscal Year: 2005
    ..abstract_text> ..
  5. Inner Membrane Protein Assembly in Bacteria
    Ross Dalbey; Fiscal Year: 2009
    ..These studies should impact the field of bacterial membrane protein biogenesis and advance the knowledge of analogous pathways in eukaryotic cells. ..
  6. Studying GrpE: Protein Function, Stability, and Folding
    ANDREW MEHL; Fiscal Year: 2002
    ..Point mutants will also be created to test the function of salt bridges within the dimer interface. An internal deletion mutant is proposed that will potentially lead to only a monomer of GrpE. ..
  7. Pathogenesis of EAST1 toxin in ETEC associated diarrhea disease
    Weiping Zhang; Fiscal Year: 2008
    ..The determination of virulence significance of EAST1 toxin in diarrheal disease will provide essential information to prevent or control this disease. [unreadable] [unreadable] [unreadable] [unreadable]..
  8. Development of an additive model to study the significance of heat-labile and hea
    Weiping Zhang; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  9. GENETIC CONTROL OF NITRATE RESPIRATION IN E. COLI
    Valley J Stewart; Fiscal Year: 2010
    ..Thus, results from the model species E. coli enhance understanding for a broad range of pathogens that significantly impact public health. ..
  10. Glucose Signaling: Regulation of Enzyme Activity
    DONALD PETTIGREW; Fiscal Year: 2006
    ..coli. ..
  11. Genome-wide screening of E. coli for bacteremia
    Kwang Kim; Fiscal Year: 2007
    ..coli bacteremia should help in development of preventive measures such as vaccines against E. coli bacteremia. [unreadable] [unreadable] [unreadable]..
  12. Role of phage in expression & transmission of STX genes
    David Friedman; Fiscal Year: 2008
    ..unreadable] [unreadable]..
  13. BACTERIAL CHEMOTAXIS: A COMPUTER-BASED STUDY
    Dennis Bray; Fiscal Year: 2007
    ..Moreover, the intimate involvement of computational and experimental approaches in our study will both contribute to, and derive benefit from, the raidly expanding area of systems biology. [unreadable] [unreadable]..
  14. MECHANISMS OF ESCHERICHIA COLI CHROMOSOMAL REPLICATION
    JON KAGUNI; Fiscal Year: 2008
    ..Further study on the molecular mechanism of initiation and its regulation in E. coli should provide insight into the biochemistry of initiation and its control in other organisms. [unreadable] [unreadable]..
  15. HIV INFECTION AND OPIOID-INDUCED IMMUNOREGULATION
    Thomas Rogers; Fiscal Year: 2007
    ..We expect the proposed studies will enhance our understanding of the role of opioid drugs of abuse in the interaction of the immune system with HIV. ..
  16. Structure and mechanism of the ABC maltose transporter
    Jue Chen; Fiscal Year: 2008
    ..coli maltose transport mechanism will advance our understanding of how conformational changes of ABC transporters facilitate the transport processes. ..
  17. The eae Gene Cluster of Enteropathogenic E.coli
    Michael Donnenberg; Fiscal Year: 2007
    ..The significance of this work is that it will lead to a better understanding of how bacteria inject proteins into host cells and interfere with pathways that protect the cells from death and maintain barrier function. ..
  18. Modeling Min-protein oscillations in bacteria
    Ned Wingreen; Fiscal Year: 2008
    ....
  19. Structural Studies of AIDS-Responsive Drugs
    Vivian Cody; Fiscal Year: 2010
    ..These results will help guide the design of species selective inhibitors. Mutagenesis studies will be carried out to test these possibilitiesin the structure-based correlations to help design novel pjDHFRinhibitors. ..
  20. Structural Studies of Transcriptional Regulators
    Mitchell Lewis; Fiscal Year: 2011
    ..In the future a modified lac repressor could be used to regulate a variety of promoters. Endogenous loci could be switched on and off to create reversible models of human disease and normal development. ..
  21. TERMINAL ENZYMES OF HEME SYNTHESIS
    Harry A Dailey; Fiscal Year: 2010
    ..3.) identify and characterize protein-protein interactions between ferrochelatase and PPO. ..
  22. The structural basis for APP cleavability
    Ya Ha; Fiscal Year: 2009
    ..abstract_text> ..
  23. Spatio-temporal analysis of HIV-1 assembly and release
    Markus Thali; Fiscal Year: 2008
    ..abstract_text> ..
  24. TETHERED DOMAINS AS REGULATORY ELEMENTS
    Gregory Grant; Fiscal Year: 2009
    ..Our observations that PGDH from M. tuberculosis is inhibited by L-serine and that PGDH from humans is not, may provide a new focus in drug development for treatment of tuberculosis. ..
  25. MOLECULAR BASIS OF LOCALIZED ADHERENCE IN E. COLI
    Michael Donnenberg; Fiscal Year: 2009
    ..The structure of the distantly related B6 variant of bundlin will be solved to shed light on the role of sequence variation in pilus structure and immunogenicity. ..
  26. Centrifugal Precipitation Chromatography for Biotechnology
    Martha Knight; Fiscal Year: 2008
    ..New manufacturing techniques will be applied to make an improved instrument for introduction on the market. [unreadable] [unreadable] [unreadable]..
  27. Ler control of EPEC virulence
    JAY MELLIES; Fiscal Year: 2008
    ..Further elucidation of the molecular mechanisms of Ler action should lead to the development of chemotherapeutic agents directed against EPEC and related E. coli O157 bacteria. [unreadable] [unreadable] [unreadable] [unreadable]..
  28. Biophysical Studies into the Coronavirus Packaging Mechanism
    Jue Chen; Fiscal Year: 2008
    ..This application aims at establishing a general model for coronavirus assembly. Knowledge gained in this study will be useful for the development of antiviral drugs. [unreadable] [unreadable] [unreadable]..
  29. Development of killed vaccine for Vibrio cholerae O139
    Jan Holmgren; Fiscal Year: 2007
    ..unreadable] [unreadable]..
  30. Typhoon Trio Imager
    Carol Wilusz; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  31. OPIOIDS AND T CELL DIFFERENTIATION
    Thomas Rogers; Fiscal Year: 2001
    ..It is hoped that these studies will provide valuable information relating to the control of the T cell arm of the immune system in opioid abuse. ..
  32. Ethanol, Tat and the Blood-brain barrier
    Kwang Kim; Fiscal Year: 2004
    ..e., nitric oxide and signaling pathways. The information derived from this proposal should enhance our understanding of the role of ethanol in HIV-1 neuropthogenesis. ..
  33. DNA Replication Termination in Escherichia coli.
    CHARMAIN COURCELLE; Fiscal Year: 2005
    ..These experiments are necessary to characterize this critical, yet heretofore unexamined, mechanism for accurate chromosomal replication. ..
  34. LENTIVIRUS-HOST INTERACTIONS DURING ASSEMBLY AND RELEASE
    Markus Thali; Fiscal Year: 2005
    ....
  35. Type IV Pilins as Vaccines against Bioterrorism Threats
    Michael Donnenberg; Fiscal Year: 2004
    ....
  36. Imaging HIV-1 Egress by Evanescent Field Microscopy
    Markus Thali; Fiscal Year: 2005
    ..We anticipate that EF-FM/TIR-FM will become an important tool to elucidate, with high spatial and temporal resolution, the sequence of events culminating in the release of newly formed viral particles. ..
  37. 10th-12th Conferences: Drug Abuse/Immunomodulation/AIDS
    Thomas Rogers; Fiscal Year: 2005
    ..The combination of the web site, and a brochure mailing should guarantee wide dissemination of information regarding the meeting, and guarantee publicity for the published results. ..
  38. Resistant E.coli in Humans and Poultry
    James Johnson; Fiscal Year: 2005
    ..Does the research address a current and compelling problem of antimicrobial resistance that is of high public health importance and for which research is needed? [yes]. ..
  39. FASEB SUMMER RESEARCH CONFERENCE: TRANSPORT ATPASES
    ALAN SENIOR; Fiscal Year: 2005
    ..This conference, focussed on transport ATPases but multidisciplinary in nature, is therefore very clearly related to the mission of NIH. ..
  40. CONJUGATIVE MOBILIZATION OF PLASMID R1162
    Richard Meyer; Fiscal Year: 2003
    ..How pSCl0l has become independent of these proteins will investigated. Overall, these studies will provide insight into how the relaxosome directs plasmid DNA to the conjugal pore for transfer. ..
  41. Biofilm growth and detachment of an oral pathogen
    Jeffrey Kaplan; Fiscal Year: 2007
    ..abstract_text> ..
  42. Modular design of central metabolism in methylotrophs
    Gregory Crowther; Fiscal Year: 2006
    ..abstract_text> ..
  43. E. COLI BINDING TO BRAIN ENDOTHELIAL CELLS
    Kwang Kim; Fiscal Year: 2003
    ....
  44. CHARACTERIZATION OF E.COLI F1FO-ATP SYNTHASE
    ALAN SENIOR; Fiscal Year: 2004
    ..ATP-driven pumps are very widely distributed in nature, and are involved in many disease states. Work to be done here will consequently have broad impact in biology and medicine. ..
  45. Zeiss LSM510 META confocal-fluorescence spectroscopy
    HAROLD ERICKSON; Fiscal Year: 2003
    ..Projects for this module are proposed by two faculty from Cell Biology and two from Biochemistry. This will be the only FCS facility at Duke University, and we expect additional projects from across the campus. ..
  46. BIOCHEMICAL INVESTIGATION OF P-GLYCOPROTEIN
    ALAN SENIOR; Fiscal Year: 2004
    ..Basic knowledge of this kind will be invaluable in devising ways to disable P-glycoprotein and overcome drug-resistance in patients. ..
  47. Molecular mechanism of antibiotic rifampicin action
    Irina Artsimovitch; Fiscal Year: 2006
    ..Finally a set of rules for design of more potent rifamycin-like antibiotics will be formulated to achieve the long-term goal of knowledge-based creation of the new generation of antibacterial drugs. ..
  48. Screen for Quorum Sensing Molecular Inhibitors (RMI)
    Vanessa Sperandio; Fiscal Year: 2005
    ....
  49. MEMBRANE PROTEIN STRUCTURE : USE OF LIPIDIC MESOPHASES
    Martin Caffrey; Fiscal Year: 2004
    ..The results of these studies, together with an analysis in detail of the crystal/lipid interface will then be used in attempts at rational crystallization of the previously uncrystallized photosystem II from Chlamydomonas. ..
  50. BIAcore 3000 System
    Jannette Carey; Fiscal Year: 2005
    ..The instrument will also support instruction in the fundamental principles and practice of ligand-binding analysis for graduate and advanced undergraduate students. ..
  51. Export & Import of Lethal Agents Mediated by TolC
    Rajeev Misra; Fiscal Year: 2006
    ..Besides the obvious role of TolC in antibiotic resistance, its ability to mediate the secretion of a variety of virulence factors in pathogenic bacteria makes it a medically important subject of investigation. ..
  52. ENZYMATIC MECHANISMS OF SULFUR NUCLEOSIDE METABOLISM
    GEORGE MARKHAM; Fiscal Year: 2008
    ..These studies will unmask why nature conserves use of a pyruvoyl cofactor for this metabolic function instead of adopting the common pyridoxal cofactor. ..
  53. Molecular Basis for Recognition and Nuclear Import of Non-classical NLS Cargos
    Gino Cingolani; Fiscal Year: 2010
    ..Carry out a quantitative structural and functional analysis of HIV-1 Rev-NLS 3. Define the molecular basis for the recognition of STAT1 "dinner-specific"NLS by importin a-5. ..
  54. Novel Application of Infection Control Strategies to Limit Transmission of ESBL'S
    Ebbing Lautenbach; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  55. PROTON & ELECTRON TRANSFER & ENERGY COUPLING IN SITE I
    TOMOKO NONE OHNISHI; Fiscal Year: 2010
    ..Using this new strategy, we found that both cluster N2 and flavin can generate superoxide. We will solidify this exciting finding and study its relevance to complex I function under physiological and pathological conditions. ..
  56. Quinolone resistant E coli in long term care facilities
    Ebbing Lautenbach; Fiscal Year: 2009
    ..abstract_text> ..
  57. Clinical Impact of Quinolone-Resistant E.coli carriage
    Ebbing Lautenbach; Fiscal Year: 2007
    ..unreadable] [unreadable]..
  58. MECHANISM OF E. COLI TERMINATION FACTOR RHO
    Barbara Stitt; Fiscal Year: 2003
    ..The results will provide a better understanding of how the energy of ATP hydrolysis can be transduced to movement. ..
  59. Hydrophobics transport across the outer membrane
    Bert van den Berg; Fiscal Year: 2009
    ..These structures will also allow us to determine whether mechanistic differences exist between LCFA and xenobiotics transport. ..
  60. Cryptococcus traversal of Brain Endothelial cells
    Kwang Kim; Fiscal Year: 2007
    ..neoformans traversal of HBMEC monolayers and will be the basis of future investigations to characterize microbial-host interactions that are relevant to the pathogenesis of cryptococcal meningitis/meningoencephalitis. ..
  61. GONOCOCCI: GENETICS OF RESISTANCE TO PMN PROTEINS
    William Shafer; Fiscal Year: 2009
    ..abstract_text> ..
  62. GP 120 MEDIATED ACTIVATION OF BRAIN ENDOTHELIAL CELLS
    Kwang Kim; Fiscal Year: 2002
    ..e., roles of G proteins and signaling pathways, platelet-activating factor (PAF). The information derived from this proposal should enhance our understanding of the role of HBMEC in HIV encephalopathy. ..
  63. E. coli invasion of brain endothelial cells
    Kwang Kim; Fiscal Year: 2008
    ..3.To determine the role of K1 capsule in E. coli trafficking in HBMEC. Further understanding and characterization of E. coli K1-HBMEC interactions should allow us to develop novel strategies to prevent this serious infection. ..
  64. Gonococcal Pilus Structure and Function
    H Seifert; Fiscal Year: 2008
    ..These studies will provide new findings and insights into the structure and functions of the gonococcal pilus. ..