bacterial proteins

Summary

Summary: Proteins found in any species of bacterium.

Top Publications

  1. pmc Dali server: conservation mapping in 3D
    Liisa Holm
    Institute of Biotechnology and Department of Biosciences, University of Helsinki, Helsinki, Finland
    Nucleic Acids Res 38:W545-9. 2010
  2. ncbi Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes
    A Krogh
    Center for Biological Sequence Analysis, Technical University of Denmark, Building 208, 2800 Lyngby, Denmark
    J Mol Biol 305:567-80. 2001
  3. pmc Hfq and its constellation of RNA
    Jörg Vogel
    Institute for Molecular Infection Biology, University of Wurzburg, Joseph Schneider Strasse, Wurzburg, Germany
    Nat Rev Microbiol 9:578-89. 2011
  4. pmc Evolution and classification of the CRISPR-Cas systems
    Kira S Makarova
    National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, 8600 Rockville Pike, Bethesda, Maryland 20894, USA
    Nat Rev Microbiol 9:467-77. 2011
  5. pmc CRISPR RNA maturation by trans-encoded small RNA and host factor RNase III
    Elitza Deltcheva
    The Laboratory for Molecular Infection Medicine Sweden, Umea, Sweden
    Nature 471:602-7. 2011
  6. doi Breaking the code of DNA binding specificity of TAL-type III effectors
    Jens Boch
    Department of Genetics, Institute of Biology, Martin Luther University Halle Wittenberg, Weinbergweg 10, D 06099 Halle Saale Germany
    Science 326:1509-12. 2009
  7. doi A simple cipher governs DNA recognition by TAL effectors
    Matthew J Moscou
    Department of Plant Pathology and Bioinformatics and Computational Biology Program, Iowa State University, Ames, IA 50011, USA
    Science 326:1501. 2009
  8. pmc Structure and regulation of the type VI secretion system
    Julie M Silverman
    Department of Microbiology, University of Washington, Seattle, 98195, USA
    Annu Rev Microbiol 66:453-72. 2012
  9. ncbi Complete genome sequence of the model actinomycete Streptomyces coelicolor A3(2)
    S D Bentley
    The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK
    Nature 417:141-7. 2002
  10. pmc Unification of Cas protein families and a simple scenario for the origin and evolution of CRISPR-Cas systems
    Kira S Makarova
    National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, 8600 Rockville Pike, Bethesda, MD 20894, USA
    Biol Direct 6:38. 2011

Detail Information

Publications374 found, 100 shown here

  1. pmc Dali server: conservation mapping in 3D
    Liisa Holm
    Institute of Biotechnology and Department of Biosciences, University of Helsinki, Helsinki, Finland
    Nucleic Acids Res 38:W545-9. 2010
    ..In favourable cases, protein structure comparison can lead to evolutionary discoveries not detected by sequence analysis...
  2. ncbi Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes
    A Krogh
    Center for Biological Sequence Analysis, Technical University of Denmark, Building 208, 2800 Lyngby, Denmark
    J Mol Biol 305:567-80. 2001
    ..We discuss the possible relevance of this finding for our understanding of membrane protein assembly mechanisms. A TMHMM prediction service is available at http://www.cbs.dtu.dk/services/TMHMM/...
  3. pmc Hfq and its constellation of RNA
    Jörg Vogel
    Institute for Molecular Infection Biology, University of Wurzburg, Joseph Schneider Strasse, Wurzburg, Germany
    Nat Rev Microbiol 9:578-89. 2011
    ..In this Review, we describe the salient structural and functional features of Hfq and discuss possible mechanisms by which this protein can promote RNA interactions to catalyse specific and rapid regulatory responses in vivo...
  4. pmc Evolution and classification of the CRISPR-Cas systems
    Kira S Makarova
    National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, 8600 Rockville Pike, Bethesda, Maryland 20894, USA
    Nat Rev Microbiol 9:467-77. 2011
    ..Accordingly, we propose a 'polythetic' classification that integrates the phylogenies of the most common cas genes, the sequence and organization of the CRISPR repeats and the architecture of the CRISPR-cas loci...
  5. pmc CRISPR RNA maturation by trans-encoded small RNA and host factor RNase III
    Elitza Deltcheva
    The Laboratory for Molecular Infection Medicine Sweden, Umea, Sweden
    Nature 471:602-7. 2011
    ..pyogenes against prophage-derived DNA. Our study reveals a novel pathway of small guide RNA maturation and the first example of a host factor (RNase III) required for bacterial RNA-mediated immunity against invaders...
  6. doi Breaking the code of DNA binding specificity of TAL-type III effectors
    Jens Boch
    Department of Genetics, Institute of Biology, Martin Luther University Halle Wittenberg, Weinbergweg 10, D 06099 Halle Saale Germany
    Science 326:1509-12. 2009
    ..Our study describes the functionality of a distinct type of DNA binding domain and allows the design of DNA binding domains for biotechnology...
  7. doi A simple cipher governs DNA recognition by TAL effectors
    Matthew J Moscou
    Department of Plant Pathology and Bioinformatics and Computational Biology Program, Iowa State University, Ames, IA 50011, USA
    Science 326:1501. 2009
    ..Our finding represents a previously unknown mechanism for protein-DNA recognition that explains TAL effector specificity, enables target site prediction, and opens prospects for use of TAL effectors in research and biotechnology...
  8. pmc Structure and regulation of the type VI secretion system
    Julie M Silverman
    Department of Microbiology, University of Washington, Seattle, 98195, USA
    Annu Rev Microbiol 66:453-72. 2012
    ..This review discusses recent progress toward characterizing T6S structure and regulation...
  9. ncbi Complete genome sequence of the model actinomycete Streptomyces coelicolor A3(2)
    S D Bentley
    The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK
    Nature 417:141-7. 2002
    ..The genome sequence will greatly increase our understanding of microbial life in the soil as well as aiding the generation of new drug candidates by genetic engineering...
  10. pmc Unification of Cas protein families and a simple scenario for the origin and evolution of CRISPR-Cas systems
    Kira S Makarova
    National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, 8600 Rockville Pike, Bethesda, MD 20894, USA
    Biol Direct 6:38. 2011
    ..Comparative analysis of the Cas protein sequences and structures led to the classification of the CRISPR-Cas systems into three Types (I, II and III)...
  11. ncbi AQUA and PROCHECK-NMR: programs for checking the quality of protein structures solved by NMR
    R A Laskowski
    Department of Biochemistry and Molecular Biology, University College London, UK
    J Biomol NMR 8:477-86. 1996
    ..They are intended to be of use both to support ongoing NMR structure determination and in the validation of the final results...
  12. pmc Genetic adaptation by Pseudomonas aeruginosa to the airways of cystic fibrosis patients
    Eric E Smith
    Genome Center, Program in Molecular and Cellular Biology, University of Washington, Seattle, WA 98195, USA
    Proc Natl Acad Sci U S A 103:8487-92. 2006
    ..aeruginosa strains present in advanced CF infections differ systematically from those of "wild-type" P. aeruginosa and that these differences may offer new opportunities for treatment of this chronic disease...
  13. pmc A phylogeny-driven genomic encyclopaedia of Bacteria and Archaea
    Dongying Wu
    DOE Joint Genome Institute, Walnut Creek, California 94598, USA
    Nature 462:1056-60. 2009
    ....
  14. pmc A type VI secretion system of Pseudomonas aeruginosa targets a toxin to bacteria
    Rachel D Hood
    Department of Microbiology, University of Washington, Seattle, WA 98195, USA
    Cell Host Microbe 7:25-37. 2010
    ..This demonstration that the T6SS targets a toxin to bacteria helps reconcile the structural and evolutionary relationship between the T6SS and the bacteriophage tail and spike...
  15. pmc Type VI secretion requires a dynamic contractile phage tail-like structure
    M Basler
    Department of Microbiology and Immunobiology, Harvard Medical School, 200 Longwood Avenue, Boston, Massachusetts 02115, USA
    Nature 483:182-6. 2012
    ..These data support a model in which the contraction of the type VI secretion system sheath provides the energy needed to translocate proteins out of effector cells and into adjacent target cells...
  16. doi Creation of a bacterial cell controlled by a chemically synthesized genome
    Daniel G Gibson
    The J Craig Venter Institute, 9704 Medical Center Drive, Rockville, MD 20850, USA
    Science 329:52-6. 2010
    ..The new cells have expected phenotypic properties and are capable of continuous self-replication...
  17. ncbi Mechanisms of biofilm resistance to antimicrobial agents
    T F Mah
    Dept of Microbiology and Immunology, Dartmouth Medical School, Hanover, NH 03755, USA
    Trends Microbiol 9:34-9. 2001
    ..Recent research has begun to shed light on how and why surface-attached microbial communities develop resistance to antimicrobial agents...
  18. pmc Type VI secretion delivers bacteriolytic effectors to target cells
    Alistair B Russell
    Department of Microbiology, University of Washington, Seattle, Washington 98195, USA
    Nature 475:343-7. 2011
    ..The requirement for these immunity proteins depends on intercellular self-intoxication through an active type VI secretion system, indicating a mechanism for export whereby effectors do not access donor cell periplasm in transit...
  19. pmc A virulence locus of Pseudomonas aeruginosa encodes a protein secretion apparatus
    Joseph D Mougous
    Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA 02115, USA
    Science 312:1526-30. 2006
    ..Thus, HSI-I likely contributes to the pathogenesis of P. aeruginosa in CF patients. HSI-I-related loci are widely distributed among bacterial pathogens and may play a general role in mediating host interactions...
  20. pmc Pseudomonas Genome Database: improved comparative analysis and population genomics capability for Pseudomonas genomes
    Geoffrey L Winsor
    Department of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, BC V5A 1S6, Canada
    Nucleic Acids Res 39:D596-600. 2011
    ....
  21. ncbi HADDOCK: a protein-protein docking approach based on biochemical or biophysical information
    Cyril Dominguez
    Department of NMR Spectroscopy, Bijvoet Center for Biomolecular Research, Utrecht University, 3584CH, Utrecht, The Netherlands
    J Am Chem Soc 125:1731-7. 2003
    ..In all cases, the best structures generated by HADDOCK, that is, the structures with the lowest intermolecular energies, were the closest to the published structure of the respective complexes (within 2.0 A backbone RMSD)...
  22. pmc Cas9-crRNA ribonucleoprotein complex mediates specific DNA cleavage for adaptive immunity in bacteria
    Giedrius Gasiunas
    Institute of Biotechnology, Vilnius University, Vilnius, Lithuania
    Proc Natl Acad Sci U S A 109:E2579-86. 2012
    ..These findings pave the way for engineering of universal programmable RNA-guided DNA endonucleases...
  23. pmc Sequence- and structure-specific RNA processing by a CRISPR endonuclease
    Rachel E Haurwitz
    Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA
    Science 329:1355-8. 2010
    ..The RNA recognition mechanism identified here explains sequence- and structure-specific processing by a large family of CRISPR-specific endoribonucleases...
  24. ncbi Community genomics among stratified microbial assemblages in the ocean's interior
    Edward F DeLong
    Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Science 311:496-503. 2006
    ..Comparative genomic analyses of stratified microbial communities have the potential to provide significant insight into higher-order community organization and dynamics...
  25. pmc The crystal structure of a voltage-gated sodium channel
    Jian Payandeh
    Department of Pharmacology, University of Washington, Seattle, Washington 98195, USA
    Nature 475:353-8. 2011
    ..This structure provides the template for understanding electrical signalling in excitable cells and the actions of drugs used for pain, epilepsy and cardiac arrhythmia at the atomic level...
  26. pmc The HHpred interactive server for protein homology detection and structure prediction
    Johannes Söding
    Department of Protein Evolution, Max Planck Institute for Developmental Biology Spemannstrasse 35, 72076 Tubingen, Germany
    Nucleic Acids Res 33:W244-8. 2005
    ..A detailed help facility is available. As a demonstration, we analyze the sequence of SpoVT, a transcriptional regulator from Bacillus subtilis. HHpred can be accessed at http://protevo.eb.tuebingen.mpg.de/hhpred...
  27. pmc Pretreatment of mice with streptomycin provides a Salmonella enterica serovar Typhimurium colitis model that allows analysis of both pathogen and host
    Manja Barthel
    Institute of Microbiology, ETH Zurich, 8092 Zurich, Switzerland
    Infect Immun 71:2839-58. 2003
    ..Our results demonstrate that streptomycin-pretreated mice offer a unique infection model that allows for the first time to use mutants of both the pathogen and the host to study the molecular mechanisms of enteric salmonellosis...
  28. ncbi Quorum sensing: cell-to-cell communication in bacteria
    Christopher M Waters
    Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544 1014, USA
    Annu Rev Cell Dev Biol 21:319-46. 2005
    ....
  29. ncbi Complete genome sequence of a virulent isolate of Streptococcus pneumoniae
    H Tettelin
    The Institute for Genomic Research (TIGR, 9712 Medical Center Drive, Rockville, MD 20850, USA
    Science 293:498-506. 2001
    ..Comparative genome hybridization with DNA arrays revealed strain differences in S. pneumoniae that could contribute to differences in virulence and antigenicity...
  30. ncbi Partitioning of lipid-modified monomeric GFPs into membrane microdomains of live cells
    David A Zacharias
    Department of Pharmacology, Biomedical Sciences Graduate Program, and, Howard Hughes Medical Institute, University of California, San Diego, La Jolla, CA 92093 0647, USA
    Science 296:913-6. 2002
    ..Thus the nature of the lipid anchor on a protein is sufficient to determine submicroscopic localization within the plasma membrane...
  31. ncbi Bacterial iron homeostasis
    Simon C Andrews
    School of Animal and Microbial Sciences, University of Reading, Reading RG6 6AJ, UK
    FEMS Microbiol Rev 27:215-37. 2003
    ....
  32. pmc Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter
    L M Guzman
    Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Bacteriol 177:4121-30. 1995
    ..We have exploited the tight regulation of the PBAD promoter to study the phenotypes of null mutations of essential genes and explored the use of pBAD vectors as an expression system...
  33. ncbi IL-23 and IL-17 in the establishment of protective pulmonary CD4+ T cell responses after vaccination and during Mycobacterium tuberculosis challenge
    Shabaana A Khader
    Trudeau Institute, Saranac Lake, New York 12983, USA
    Nat Immunol 8:369-77. 2007
    ....
  34. pmc Cellular defenses against superoxide and hydrogen peroxide
    James A Imlay
    Department of Microbiology, University of Illinois, Urbana, IL 61801, USA
    Annu Rev Biochem 77:755-76. 2008
    ..Current research seeks to identify these, and bacteria comprise an exceptionally accessible experimental system that has provided many of the answers. This manuscript reviews recent developments and identifies remaining puzzles...
  35. ncbi Neighbor-net: an agglomerative method for the construction of phylogenetic networks
    David Bryant
    McGill Centre for Bioinformatics, Montreal, Quebec, Canada
    Mol Biol Evol 21:255-65. 2004
    ..and a collection of 12 Archeal chaperonin sequences demonstrating strong evidence for gene conversion. Neighbor-Net is available as part of the SplitsTree4 software package...
  36. pmc RNA-guided complex from a bacterial immune system enhances target recognition through seed sequence interactions
    Blake Wiedenheft
    Howard Hughes Medical Institute, University of California, Berkeley, CA 94720, USA
    Proc Natl Acad Sci U S A 108:10092-7. 2011
    ....
  37. pmc Biological diversity of prokaryotic type IV secretion systems
    Cristina E Alvarez-Martinez
    Department of Microbiology and Molecular Genetics, University of Texas Medical School at Houston, 6431 Fannin, Houston, TX 77030, USA
    Microbiol Mol Biol Rev 73:775-808. 2009
    ....
  38. pmc Dynamic evolution of pathogenicity revealed by sequencing and comparative genomics of 19 Pseudomonas syringae isolates
    David A Baltrus
    Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA
    PLoS Pathog 7:e1002132. 2011
    ..This megaplasmid encodes a type IV secretion system and a diverse set of unknown proteins, which dramatically increases both the genomic content of these strains and the pan-genome of the species...
  39. doi Wolbachia: master manipulators of invertebrate biology
    John H Werren
    Biology Department, University of Rochester, Rochester, New York, New York 14627, USA
    Nat Rev Microbiol 6:741-51. 2008
    ..Here, we review the basic biology of Wolbachia, with emphasis on recent advances in our understanding of these fascinating endosymbionts...
  40. pmc Expanded dynamic range of fluorescent indicators for Ca(2+) by circularly permuted yellow fluorescent proteins
    Takeharu Nagai
    Laboratory for Cell Function and Dynamics, Advanced Technology Development Group, Brain Science Institute, RIKEN, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
    Proc Natl Acad Sci U S A 101:10554-9. 2004
    ..Our study provides an important guide for the development and improvement of indicators using GFP-based FRET...
  41. pmc Lipopolysaccharide endotoxins
    Christian R H Raetz
    Department of Biochemistry, Duke University Medical Center, Durham, North Carolina 27710, USA
    Annu Rev Biochem 71:635-700. 2002
    ..The expanding knowledge of TLR4 specificity and its downstream signaling pathways should provide new opportunities for blocking inflammation associated with infection...
  42. pmc Community-associated methicillin-resistant Staphylococcus aureus: epidemiology and clinical consequences of an emerging epidemic
    Michael Z David
    Department of Pediatrics and Department of Medicine, The University of Chicago, 5841 S Maryland Ave, Chicago, IL 60637, USA
    Clin Microbiol Rev 23:616-87. 2010
    ..It also addresses the therapy of these infections and strategies for their prevention...
  43. ncbi Two-component signal transduction
    A M Stock
    Center for Advanced Biotechnology and Medicine and Howard Hughes Medical Institute, University of Medicine and Dentistry of New Jersey Robert Wood Johnson Medical School, Piscataway, New Jersey 08854, USA
    Annu Rev Biochem 69:183-215. 2000
    ..Thus detailed analyses of a relatively small number of representative proteins provide a foundation for understanding this large family of signaling proteins...
  44. pmc Comparative genomics of the lactic acid bacteria
    K Makarova
    National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
    Proc Natl Acad Sci U S A 103:15611-6. 2006
    ....
  45. ncbi A predominantly clonal multi-institutional outbreak of Clostridium difficile-associated diarrhea with high morbidity and mortality
    Vivian G Loo
    Department of Microbiology, McGill University Health Center, Montreal, Que, Canada
    N Engl J Med 353:2442-9. 2005
    ..In March 2003, several hospitals in Quebec, Canada, noted a marked increase in the incidence of Clostridium difficile-associated diarrhea...
  46. ncbi Structure and mechanism of helicases and nucleic acid translocases
    Martin R Singleton
    Macromolecular Structure and Function Laboratory, The London Research Institute, London WC2A 3PX, United Kingdom
    Annu Rev Biochem 76:23-50. 2007
    ..As a result, each superfamily is subdivided, where appropriate, on the basis of mechanistic understanding, which we hope will provide a framework for classification of new superfamily members as they are discovered and characterized...
  47. ncbi Evaluation of a nutrient starvation model of Mycobacterium tuberculosis persistence by gene and protein expression profiling
    Joanna C Betts
    Respiratory Pathogens, GlaxoSmithKline, Stevenage, Herts, UK
    Mol Microbiol 43:717-31. 2002
    ..Thus, we have generated a model with which we can search for agents active against persistent M. tuberculosis and revealed a number of potential targets expressed under these conditions...
  48. pmc A small RNA regulates the expression of genes involved in iron metabolism in Escherichia coli
    Eric Massé
    Laboratory of Molecular Biology, National Cancer Institute, Building 37, Room 5132, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 99:4620-5. 2002
    ....
  49. pmc The Streptococcus thermophilus CRISPR/Cas system provides immunity in Escherichia coli
    Rimantas Sapranauskas
    Institute of Biotechnology, Vilnius University, Graiciuno 8, LT 02241 Vilnius, Lithuania
    Nucleic Acids Res 39:9275-82. 2011
    ..This can be leveraged to develop strains more robust against phage attack, and safer organisms less likely to uptake and disseminate plasmid-encoded undesirable genetic elements...
  50. ncbi The multidrug-resistant human pathogen Clostridium difficile has a highly mobile, mosaic genome
    Mohammed Sebaihia
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, CB10 1SA, UK
    Nat Genet 38:779-86. 2006
    ..The extreme genome variability was confirmed by whole-genome microarray analysis; it may reflect the organism's niche in the gut and should provide information on the evolution of virulence in this organism...
  51. ncbi Quorum sensing in bacteria
    M B Miller
    Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544 1014, USA
    Annu Rev Microbiol 55:165-99. 2001
    ..Presumably, this process bestows upon bacteria some of the qualities of higher organisms. The evolution of quorum sensing systems in bacteria could, therefore, have been one of the early steps in the development of multicellularity...
  52. ncbi A signaling network reciprocally regulates genes associated with acute infection and chronic persistence in Pseudomonas aeruginosa
    Andrew L Goodman
    Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA 02115, USA
    Dev Cell 7:745-54. 2004
    ..This study defines RetS as a pleiotropic regulator of multiple virulence phenotypes that orchestrates genes required for acute infection and genes associated with chronic persistence...
  53. pmc Identification of a conserved bacterial protein secretion system in Vibrio cholerae using the Dictyostelium host model system
    Stefan Pukatzki
    Department of Microbiology and Molecular Genetics, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 103:1528-33. 2006
    ..Thus, the genes encoding the VAS-related, type VI secretion system likely play an important conserved function in microbial pathogenesis and represent an additional class of targets for vaccine and antimicrobial drug-based therapies...
  54. pmc Prediction of lipoprotein signal peptides in Gram-negative bacteria
    Agnieszka S Juncker
    Center for Biological Sequence Analysis, Technical University of Denmark, Lyngby 2800, Denmark
    Protein Sci 12:1652-62. 2003
    ..A neural network-based predictor was developed for comparison, and it gave very similar results. LipoP is available as a Web server at www.cbs.dtu.dk/services/LipoP/...
  55. ncbi Neutrophil extracellular traps kill bacteria
    Volker Brinkmann
    Microscopy Core Facility, Max Planck Institute for Infection Biology, Schumannstrasse 21 22, 10117 Berlin, Germany
    Science 303:1532-5. 2004
    ..NETs appear to be a form of innate response that binds microorganisms, prevents them from spreading, and ensures a high local concentration of antimicrobial agents to degrade virulence factors and kill bacteria...
  56. doi Bacterial nucleoid-associated proteins, nucleoid structure and gene expression
    Shane C Dillon
    Department of Microbiology, School of Genetics and Microbiology, Moyne Institute of Preventive Medicine, Trinity College, Dublin 2, Ireland
    Nat Rev Microbiol 8:185-95. 2010
    ..Here, NAP biology is considered from the standpoints of both gene regulation and nucleoid structure...
  57. ncbi An improved cyan fluorescent protein variant useful for FRET
    Mark A Rizzo
    Nat Biotechnol 22:445-9. 2004
    ..Cerulean is 2.5-fold brighter than ECFP and replacement of ECFP with Cerulean substantially improves the signal-to-noise ratio of a FRET-based sensor for glucokinase activation...
  58. ncbi The complete genome sequence of Escherichia coli K-12
    F R Blattner
    Laboratory of Genetics, University of Wisconsin Madison, 445 Henry Mall, Madison, WI 53706, USA
    Science 277:1453-62. 1997
    ..The genome also contains insertion sequence (IS) elements, phage remnants, and many other patches of unusual composition indicating genome plasticity through horizontal transfer...
  59. ncbi Cytoplasmic flagellin activates caspase-1 and secretion of interleukin 1beta via Ipaf
    Edward A Miao
    Institute for Systems Biology, Seattle, Washington 98103, USA
    Nat Immunol 7:569-75. 2006
    ....
  60. doi The real threat of Klebsiella pneumoniae carbapenemase-producing bacteria
    Patrice Nordmann
    Service de Bactériologie Virologie Hygiène, INSERM U914 Emerging Resistance to Antibiotics, Hopital de Bicetre, Assistance Publique Hopitaux de Paris, Faculté de Médecine and Université Paris Sud, K Bicêtre, France
    Lancet Infect Dis 9:228-36. 2009
    ..Detection of KPC-producing bacteria may be difficult based on routine antibiotic susceptibility testing. It is therefore crucial to implement efficient infection control measures to limit the spread of these pathogens...
  61. pmc TIGRFAMs and Genome Properties: tools for the assignment of molecular function and biological process in prokaryotic genomes
    Jeremy D Selengut
    TIGR, Bioinformatics Department, 9712 Medical Center Drive, Rockville, MD 20850, USA
    Nucleic Acids Res 35:D260-4. 2007
    ..The TIGRFAMs and Genome Properties systems can be accessed at http://www.tigr.org/TIGRFAMs and http://www.tigr.org/Genome_Properties...
  62. doi Regulation and secretion of Xanthomonas virulence factors
    Daniela Büttner
    Genetics Department, Institute of Biology, Martin Luther University Halle Wittenberg, Halle Saale, Germany
    FEMS Microbiol Rev 34:107-33. 2010
    ..In this review, we summarize the current knowledge on the infection strategies and regulatory networks controlling secreted virulence factors from Xanthomonas species...
  63. pmc Structural basis for promoter-10 element recognition by the bacterial RNA polymerase σ subunit
    Andrey Feklistov
    The Rockefeller University, 1230 York Avenue, New York, NY 10065, USA
    Cell 147:1257-69. 2011
    ..These results provide a detailed structural basis for the critical roles of A(-11) and T(-7) in promoter melting and reveal important insights into the initiation of transcription bubble formation...
  64. pmc Self versus non-self discrimination during CRISPR RNA-directed immunity
    Luciano A Marraffini
    Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, 2205 Tech Drive, Evanston, Illinois 60208, USA
    Nature 463:568-71. 2010
    ..Differential complementarity outside of the spacer sequence is a built-in feature of all CRISPR systems, indicating that this mechanism is a broadly applicable solution to the self/non-self dilemma that confronts all immune pathways...
  65. doi Xanthomonas AvrBs3 family-type III effectors: discovery and function
    Jens Boch
    Department of Genetics, Martin Luther University Halle Wittenberg, D 06099 Halle Saale, Germany
    Annu Rev Phytopathol 48:419-36. 2010
    ..Specificity of TAL effectors is determined by a novel modular DNA-binding domain. Here, we describe the discovery of TAL effectors and their structure, activity, and host targets...
  66. pmc The type III secretion system of Pseudomonas aeruginosa: infection by injection
    Alan R Hauser
    Departments of MicrobiologyImmunology and Medicine, Northwestern University, Chicago, Illinois 60611, USA
    Nat Rev Microbiol 7:654-65. 2009
    ..The features of this interesting secretion system have important implications for the pathogenesis of P. aeruginosa infections and for other type III secretion systems...
  67. ncbi Protein translocation across the bacterial cytoplasmic membrane
    Arnold J M Driessen
    Department of Molecular Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute and the Zernike Institute for Advanced Materials, University of Groningen, Haren, The Netherlands
    Annu Rev Biochem 77:643-67. 2008
    About 25% to 30% of the bacterial proteins function in the cell envelope or outside of the cell...
  68. pmc Simultaneous assay of every Salmonella Typhi gene using one million transposon mutants
    Gemma C Langridge
    The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, United Kingdom
    Genome Res 19:2308-16. 2009
    ..Typhi in the gall bladder. This screen validated our hypothesis that we can simultaneously assay every gene in the genome to identify niche-specific essential genes...
  69. pmc Plasmid-mediated quinolone resistance: a multifaceted threat
    Jacob Strahilevitz
    Department of Clinical Microbiology and Infectious Diseases, Hadassah Ein Kerem, Jerusalem 91120, Israel
    Clin Microbiol Rev 22:664-89. 2009
    ..Of concern, these genes are not yet being taken into account in resistance screening by clinical microbiology laboratories...
  70. doi Ongoing revolution in bacteriology: routine identification of bacteria by matrix-assisted laser desorption ionization time-of-flight mass spectrometry
    Piseth Seng
    Pôle des Maladies Infectieuses, Assistance Publique Hopitaux de Marseille, URMITE, Unite Mixte de Recherche, Centre National de la Recherche Scientifique Institut pour la Recherche et le Développement 6236, Universite de la Mediterranee, Marseille, France
    Clin Infect Dis 49:543-51. 2009
    ..Matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry accurately identifies both selected bacteria and bacteria in select clinical situations. It has not been evaluated for routine use in the clinic...
  71. pmc Detection of staphylococcal cassette chromosome mec type XI carrying highly divergent mecA, mecI, mecR1, blaZ, and ccr genes in human clinical isolates of clonal complex 130 methicillin-resistant Staphylococcus aureus
    Anna C Shore
    Microbiology Research Unit, Dublin Dental University Hospital, University of Dublin, Trinity College Dublin, Dublin, Ireland
    Antimicrob Agents Chemother 55:3765-73. 2011
    ..aureus strains were predominantly from bovine sources. The highly divergent nature of SCCmec XI relative to other SCCmec elements indicates that it may have originated in another taxon...
  72. ncbi Identification of genes that are associated with DNA repeats in prokaryotes
    Ruud Jansen
    Department of Infectious Diseases and Immunology, Bacteriology Division, Veterinary Faculty, Utrecht University, Yalelaan 1, 3584 CL Utrecht, The Netherlands
    Mol Microbiol 43:1565-75. 2002
    ..The spatial coherence of CRISPR and cas genes may stimulate new research on the genesis and biological role of these repeats and genes...
  73. pmc HAMAP: a database of completely sequenced microbial proteome sets and manually curated microbial protein families in UniProtKB/Swiss-Prot
    Tania Lima
    Swiss Prot group, Swiss Institute of Bioinformatics, 1 rue Michel Servet, 1211 Geneva 4, Switzerland
    Nucleic Acids Res 37:D471-8. 2009
    ..Thanks to the implementation of HAMAP, more than 200,000 microbial proteins have been fully annotated in UniProtKB/Swiss-Prot (HAMAP website: http://www.expasy.org/sprot/hamap)...
  74. pmc Burkholderia type VI secretion systems have distinct roles in eukaryotic and bacterial cell interactions
    Sandra Schwarz
    Department of Microbiology, University of Washington, Seattle, Washington, United States of America
    PLoS Pathog 6:e1001068. 2010
    ....
  75. pmc What is type VI secretion doing in all those bugs?
    Sandra Schwarz
    Department of Microbiology, University of Washington, Seattle, WA 98195, USA
    Trends Microbiol 18:531-7. 2010
    ..The crucial role of T6S in bacterial interactions, along with its presence in many organisms relevant to disease, suggests that it might be a key determinant in the progression and outcome of certain human polymicrobial infections...
  76. pmc A multi-label classifier for predicting the subcellular localization of gram-negative bacterial proteins with both single and multiple sites
    Xuan Xiao
    Computer Department, Jing De Zhen Ceramic Institute, Jing De Zhen, China
    PLoS ONE 6:e20592. 2011
    ..a novel predictor called iLoc-Gneg is developed for predicting the subcellular localization of gram-positive bacterial proteins with both single-location and multiple-location sites...
  77. pmc How rhizobial symbionts invade plants: the Sinorhizobium-Medicago model
    Kathryn M Jones
    Department of Biology, Massachusetts Institute of Technology, Building 68, Room 633, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA
    Nat Rev Microbiol 5:619-33. 2007
    ....
  78. pmc Small RNA-induced mRNA degradation achieved through both translation block and activated cleavage
    Karine Prévost
    Department of Biochemistry, RNA Group, University of Sherbrooke, Sherbrooke, Quebec J1H 5N4, Canada
    Genes Dev 25:385-96. 2011
    ..Thus, beyond translation initiation block, sRNA-induced mRNA cleavage requires several unexpected steps, many of which are determined by structural features of the target mRNA...
  79. pmc The opportunistic pathogen Serratia marcescens utilizes type VI secretion to target bacterial competitors
    Sarah L Murdoch
    Division of Molecular Microbiology, College of Life Sciences, University of Dundee, United Kingdom
    J Bacteriol 193:6057-69. 2011
    ..marcescens with a very similar and active T6SS. We conclude that type VI secretion plays a crucial role in the competitiveness, and thus indirectly the virulence, of S. marcescens and other opportunistic bacterial pathogens...
  80. doi Signal processing in complex chemotaxis pathways
    Steven L Porter
    Biosciences, College of Life and Environmental Sciences, University of Exeter, Exeter, Devon EX4 4QD, UK
    Nat Rev Microbiol 9:153-65. 2011
    ....
  81. ncbi Engineering and characterization of a superfolder green fluorescent protein
    Jean Denis Pédelacq
    Bioscience Division, MS M888, Los Alamos National Laboratory, Los Alamos, New Mexico 87545, USA
    Nat Biotechnol 24:79-88. 2006
    ..X-ray crystallographic structural analyses helped explain the enhanced folding of superfolder GFP relative to folding reporter GFP...
  82. doi Basis of virulence in community-associated methicillin-resistant Staphylococcus aureus
    Michael Otto
    Laboratory of Human Bacterial Pathogenesis, National Institute of Allergy and Infectious Diseases, The National Institutes of Health, Bethesda, Maryland 20892, USA
    Annu Rev Microbiol 64:143-62. 2010
    ..All these toxins impact disease progression in animal models of USA300 infection. In contrast, the basis of virulence in other CA-MRSA epidemics, which also include PVL-negative strains, is poorly understood...
  83. doi The type VI secretion system: translocation of effectors and effector-domains
    Stefan Pukatzki
    Department of Medical Microbiology and Immunology, University of Alberta, 1 63 Medical Sciences Building, Edmonton, Alberta T6G 2H7, Canada
    Curr Opin Microbiol 12:11-7. 2009
    ..Interestingly, a number of VgrGs contain C-terminal extensions with effector-domains. Thus, the T6SS may translocate soluble effectors, as well as VgrG effector-domains...
  84. pmc Positive regulation by small RNAs and the role of Hfq
    Toby Soper
    Johns Hopkins University, Program in Cell, Molecular, Developmental Biology and Biophysics, Baltimore, MD 21218, USA
    Proc Natl Acad Sci U S A 107:9602-7. 2010
    ..These studies demonstrate that binding of Hfq to the rpoS mRNA is critical for sRNA regulation under normal conditions, but if the stability of the sRNA*mRNA complex is sufficiently high, the requirement for Hfq can be bypassed...
  85. pmc The TetR family of transcriptional repressors
    Juan L Ramos
    Department of Plant Biochemistry and Molecular and Cellular Biology, Estacion Experimental del Zaidin, Consejo Superior de Investigaciones Cientificas, Granada, Spain
    Microbiol Mol Biol Rev 69:326-56. 2005
    ..Information related to the TetR family of regulators has been updated in a database that can be accessed at www.bactregulators.org...
  86. pmc Structural basis for sequence-specific recognition of DNA by TAL effectors
    Dong Deng
    State Key Laboratory of Bio membrane and Membrane Biotechnology, Tsinghua University, Beijing 100084, China
    Science 335:720-3. 2012
    ..The 12th residue stabilizes the RVD loop, whereas the 13th residue makes a base-specific contact. Understanding DNA recognition by TAL effectors may facilitate rational design of DNA-binding proteins with biotechnological applications...
  87. doi CRISPR/Cas, the immune system of bacteria and archaea
    Philippe Horvath
    Danisco France SAS, BP10, F 86220 Dangé Saint Romain, France
    Science 327:167-70. 2010
    ..CRISPR features may be exploited for typing purposes, epidemiological studies, host-virus ecological surveys, building specific immunity against undesirable genetic elements, and enhancing viral resistance in domesticated microbes...
  88. ncbi Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen
    C K Stover
    PathoGenesis Corporation, Seattle, Washington 98119, USA
    Nature 406:959-64. 2000
    ..We propose that the size and complexity of the P. aeruginosa genome reflect an evolutionary adaptation permitting it to thrive in diverse environments and resist the effects of a variety of antimicrobial substances...
  89. ncbi Biofilms as complex differentiated communities
    P Stoodley
    Center for Biofilm Engineering, Montana State University, Bozeman 59717, USA
    Annu Rev Microbiol 56:187-209. 2002
    ..In a holistic sense both biofilm and planktonic phenotypes may be viewed as integrated components of prokaryote life...
  90. pmc Rv3133c/dosR is a transcription factor that mediates the hypoxic response of Mycobacterium tuberculosis
    Heui Dong Park
    Department of Pathobiology, University of Washington, Seattle, WA 98195, USA
    Mol Microbiol 48:833-43. 2003
    ..Together, these results demonstrate that Rv3133c/DosR is a transcription factor of the two-component response regulator class, and that it is the primary mediator of a hypoxic signal within M. tuberculosis...
  91. pmc Salmonella takes control: effector-driven manipulation of the host
    Emma J McGhie
    University of Cambridge, Department of Pathology, Tennis Court Road, Cambridge CB2 1QP, UK
    Curr Opin Microbiol 12:117-24. 2009
    ..This review focuses on the actions of the effectors on their host cell targets during each stage of Salmonella infection...
  92. doi The RpoS-mediated general stress response in Escherichia coli
    Aurélia Battesti
    Laboratory of Molecular Biology, National Cancer Institute, Bethesda, Maryland 20892, USA
    Annu Rev Microbiol 65:189-213. 2011
    ..Recent progress has led to a better, but still far from complete, understanding of how stresses lead to RpoS induction and what RpoS-dependent genes help the cell deal with the stress...
  93. pmc The meningococcal vaccine candidate neisserial surface protein A (NspA) binds to factor H and enhances meningococcal resistance to complement
    Lisa A Lewis
    Division of Infectious Diseases and Immunology, University of Massachusetts Medical School, Worcester, Massachusetts, USA
    PLoS Pathog 6:e1001027. 2010
    ..Collectively, these data identify a key complement evasion mechanism with important implications for ongoing efforts to develop meningococcal vaccines that employ fHbp as one of its components...
  94. ncbi Indole-3-acetic acid in microbial and microorganism-plant signaling
    Stijn Spaepen
    Department of Microbial and Molecular Systems, Centre of Microbial and Plant Genetics, Heverlee, Belgium
    FEMS Microbiol Rev 31:425-48. 2007
    ..This review discusses past and recent data, and emerging views on IAA, a well-known phytohormone, as a microbial metabolic and signaling molecule...
  95. pmc Pseudomonas Genome Database: facilitating user-friendly, comprehensive comparisons of microbial genomes
    Geoffrey L Winsor
    Department of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, BC V5A 1S6, Canada
    Nucleic Acids Res 37:D483-8. 2009
    ..This database aims to continue to provide a high quality, annotated genome resource for the research community and is available under an open source license...
  96. pmc Probing transcription factor dynamics at the single-molecule level in a living cell
    Johan Elf
    Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA
    Science 316:1191-4. 2007
    ..The methods and findings can be generalized to other nucleic acid binding proteins...
  97. ncbi A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications
    Takeharu Nagai
    Laboratory for Cell Function and Dynamics, Advanced Technology Development Center, Brain Science Institute, RIKEN, 2 1 Hirosawa, Wako City, Saitama, 351 0198, Japan
    Nat Biotechnol 20:87-90. 2002
    ..With the improved speed and efficiency of maturation and the increased resistance to environment, Venus will enable fluorescent labelings that were not possible before...
  98. pmc Crystal structure of an orthologue of the NaChBac voltage-gated sodium channel
    Xu Zhang
    State Key Laboratory of Bio membrane and Membrane Biotechnology, Center for Structural Biology, Tsinghua University, Beijing 100084, China
    Nature 486:130-4. 2012
    ..We propose that Na(v)Rh is in an 'inactivated' conformation. Comparison of Na(v)Rh with Na(v)Ab reveals considerable conformational rearrangements that may underlie the electromechanical coupling mechanism of voltage-gated channels...
  99. pmc Tit-for-tat: type VI secretion system counterattack during bacterial cell-cell interactions
    Marek Basler
    Department of Microbiology and Immunobiology, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA 02115, USA
    Cell 152:884-94. 2013
    ..aeruginosa, T6SS organelle assembly and lethal counterattack are regulated by a signal that corresponds to the point of attack of the T6SS apparatus elaborated by a second aggressive T6SS(+) bacterial cell. PAPERFLICK:..
  100. ncbi Genome and virulence determinants of high virulence community-acquired MRSA
    Tadashi Baba
    Department of Bacteriology, Juntendo University, 2 1 1 Hongo, Bunkyo ku, Tokyo 113 8421, Japan
    Lancet 359:1819-27. 2002
    ..By contrast with hospital-acquired MRSA, community-acquired MRSA is more susceptible to non b-lactam antibiotics. We investigated the high virulence potential of certain strains of this bacterium...
  101. pmc PSORTb 3.0: improved protein subcellular localization prediction with refined localization subcategories and predictive capabilities for all prokaryotes
    Nancy Y Yu
    Department of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, BC V5A 1S6, Canada
    Bioinformatics 26:1608-15. 2010
    ..Such improvements should preferably be encompassed in a freely available web-based predictor that can also be used as a standalone program...

Research Grants75

  1. Controlling Radiation Injury by TLR5 Agonists
    Andrei V Gudkov; Fiscal Year: 2012
    ..The proposed experiments will provide essential mechanistic insight into the previously observed radio-protective and -mitigative effects of CBLB502. ..
  2. Enzymology of Chlamydia Pathogenesis
    Dewey G McCafferty; Fiscal Year: 2013
    ..an enzyme that has emerged as a central virulence factor due to its multivariable roles in modifying host or bacterial proteins involved in lipid and membrane transport, the actin cytoskeleton, microtubule-based motors, lysosomal ..
  3. Novel immunotherapeutics for the management of otitis media due to H. influenzae
    Steven D Goodman; Fiscal Year: 2013
    ..e. hearing loss due to OM), a goal of Strategic Plan Priority Area I. ..
  4. Polymicrobial interactions in the lung.
    JENNIFER MELINDA BOMBERGER; Fiscal Year: 2010
    ..aeruginosa. Very little is known about how secreted bacterial proteins suppress the host immune response to viral pathogens, and there is minimal data elucidating how the host ..
  5. Molecular Mechanism of cell-cell spread of Listeria in polarized epithelial cells
    KEITH PATRICK IRETON; Fiscal Year: 2013
    ..Specifically, it remains unclear whether bacterial proteins exist that function after ActA to directly control protrusion formation...
  6. Biology of the HMW1 and HMW2 Adhesins of H. Influenzae
    JOSEPH W ST. GEME; Fiscal Year: 2013
    ..influenzae disease. More generally, these studies will provide fundamental insights into host-microbial relationships, bacterial protein secretion, and prokaryotic protein glycosylation. ..
  7. Research and Development of a Novel System to Produce Polysaccharide Conjugate Va
    Peng George Wang; Fiscal Year: 2013
    ....
  8. Polymicrobial Interactions in the Lung
    JENNIFER MELINDA BOMBERGER; Fiscal Year: 2013
    ..aeruginosa. Very little is known about how secreted bacterial proteins suppress the host immune response to viral pathogens, and there is minimal data elucidating how the host ..
  9. Effects of apoptotic epithelial cell engulfment on mucosal inflammation
    Peter B Ernst; Fiscal Year: 2010
    ..Aim 3: Examine the role of bacterial proteins in regulating Rac1 activation and mucosal inflammation...
  10. Studies of YlxM of Streptococcus mutans, a newly identified component of the Sign
    MATTHEW LAWRENCE WILLIAMS; Fiscal Year: 2013
    ..The relevance of bacterial membrane proteins is exemplified by the fact that nearly a third of all bacterial proteins produced are membrane-associated or secreted...
  11. TYPE II SECRETION AND LEGIONELLA PNEUMOPHILA INFECTION
    Nicholas P Cianciotto; Fiscal Year: 2013
    ..abstract_text> ..
  12. Transport and Inhibition in a Biogenic Amine Transporter
    Satinder Singh; Fiscal Year: 2009
    ..Achieving any one of these objectives would likely have significant impact in both the laboratory and in the clinic. ..
  13. Transport and Inhibition in a Biogenic Amine Transporter
    SATINDER KAUR SINGH; Fiscal Year: 2012
    ..Achieving any one of these objectives would likely have significant impact in both the laboratory and in the clinic. ..
  14. Effect of the Middle Ear Inflammation On the Inner Ear
    STEVEN SUNG KYUN JUHN; Fiscal Year: 2013
    ..Our central hypothesis is that PspA and other bacterial proteins affect the pathology of the middle and inner ears...
  15. Interactions of Enteropathogenic E Coli with Intestinal Epithelial Cells
    Gail A Hecht; Fiscal Year: 2013
    ..Cells will also be transfected to express bacterial proteins, NleH1, NleH2, or both, and then challenged with host cytokines, bacterial pro-inflammatory molecules, or ..
  16. Host-pathogen signaling in group A streptococcal infection
    Michael R Wessels; Fiscal Year: 2012
    ..We plan to study how GAS senses activation of human host defenses during infection through specialized bacterial proteins that bind and respond to human antimicrobial peptides and magnesium ions...
  17. Bordetella adenylate cyclase toxin: the role of cell interaction in toxin functio
    JOSHUA CLARK EBY; Fiscal Year: 2013
    ..Here, we will characterize the basic mechanisms of cell binding by AC toxin and show that these mechanisms affect the function of the toxin and determine its role as a virulence factor. ..
  18. Retrotranslocation of cholera toxin AI-chain from ER ot cytosol and cell response
    Jin Ah Cho; Fiscal Year: 2010
    ..Cholera remains prevalent in many parts of Asia, Africa and Latin America and outbreaks can occur in areas affected by natural disasters, wars and famines [1]. ..
  19. Maneuvering of Macrophage Function By Escherichia coli K1
    PRASADARAO V NEMANI; Fiscal Year: 2013
    ..The information from these studies will have a great impact on our understanding of the pathogenesis of E. coli meningitis and will also be useful for developing therapeutic approaches to treat and prevent meningitis. ..
  20. Structure and Function of the B. anthracis Exosporium
    Charles L Turnbough; Fiscal Year: 2010
    ..reveal generally important mechanisms of protein-protein interactions, macromolecular assembly, protein attachment to cell surfaces, glycosylation and phosphorylation of bacterial proteins, and host-pathogen interactions.
  21. Host protein prenyl transferases as modifiers of effectors of pathogenic bacteria
    ELIA WRIGHT; Fiscal Year: 2013
    ..To investigate the prevalence of prenylation of bacterial proteins, I propose to measure the reactivity of mammalian FTase and GGTase both in vivo and in vitro with peptide ..
  22. TLR-mediated Signaling Complex Formation and Regulation of Effector Functions
    Hans Haecker; Fiscal Year: 2012
    ..e. autoimmune diseases and sepsis. ..
  23. Cell Surface Protein Anchoring and Function in Gram-Positive Bacteria
    ROBERT THOMPSON CLUBB; Fiscal Year: 2013
    ..Collectively, this research will increase our understanding of the molecular basis of S. aureus pathogenesis and it could lead to new therapeutics to treat bacterial infections. ..
  24. Anti-inflammatory promoting commensal E. coli
    CAMMIE LESSER; Fiscal Year: 2013
    ..many Gram-negative bacterial pathogens utilize specialized type 3 secretion systems to deliver tens of bacterial proteins directly into host cells proteins...
  25. A Novel CNS-Penetrable Antiretroviral
    Ned C Sacktor; Fiscal Year: 2011
    ..Additionally, viral and bacterial proteins can lead to neuronal death through inflammatory responses of CNS- resident cells, even in the absence of ..
  26. Regulation of motility and organelle assembly at the Pseudomonas aeruginosa pole
    Barbara I Kazmierczak; Fiscal Year: 2012
    ..Genomics has revealed over 2000 bacterial proteins containing domains predicted to synthesize or breakdown c-di-GMP, as well as proteins predicted to bind c-di-..
  27. Evaluation of CPAF Mediated Anti-Chlamydial Immunity in the Guinea Pig
    BERNARD PRAGASH ARULANANDAM; Fiscal Year: 2012
    ..Moreover, the completion of the sequencing of the guinea pig genome will result in the development of immunological reagents for characterization, which will further facilitate the use of this animal model in the scientific community. ..
  28. Functional Characterization of Coxiella burnetii Dot/Icm Substrates
    Daniel E Voth; Fiscal Year: 2010
    ..However, the bacterial proteins mediating these events are not known. The major mode of secretion used by C...
  29. Intestinal Disease-enterocyte toxin interaction
    Wayne Lencer; Fiscal Year: 2013
    ..We have also developed the zebrafish for genetic studies and identified 13 families by forward screen as resistant to intoxication. The mutant genes in these families will be identified by positional-mapping and their function studied. ..
  30. BACTERIAL PROTEINASES IN PERIODONTAL DISEASE
    JAN S POTEMPA; Fiscal Year: 2012
    ....
  31. Selective Delivery of a novel bacterial adjuvant for multipurpose vaccination
    GREGOIRE STEPHANE LAUVAU; Fiscal Year: 2013
    ....
  32. Cocaine Degrading Enzymes From Pest Insects Of Coca Plants
    Howard H Gu; Fiscal Year: 2010
    ....
  33. MOLECULAR BIOLOGY OF VIRULENCE IN PERIODONTAL DISEASE
    EDWARD THOMAS LALLY; Fiscal Year: 2012
    ..The outcomes of toxin/membrane interactions will be assessed by biochemical, biophysical and morphological assays. ..
  34. Enteropathogenic E. coli Effects on Intestinal Function
    Gail A Hecht; Fiscal Year: 2010
    ..and does not produce toxins, but expresses a type III secretory system (TTSS) that allows translocation of bacterial proteins into host cells...
  35. Structural Aspects of Protein Synthesis in the Mammalian Mitochondria
    Rajendra K Agrawal; Fiscal Year: 2013
    ....
  36. Development of novel proteins synthesis inhibitors for MDR tuberculosis
    RICHARD EDWARD LEE; Fiscal Year: 2013
    ..Compounds discovered in this study may also have the potential to treat other drug resistant bacterial diseases. ..
  37. Characterization of an Anaplasma phagocytophilum protein interfering with eukaryo
    Sukanya Narasimhan; Fiscal Year: 2010
    ..the mammalian host cells by this gram-negative bacterium are known, relatively little is known regarding the bacterial proteins involved in exploiting the host cells to establish a successful infection...
  38. Computationally optimized anti-staphylococcal biotherapeutics
    Karl E Griswold; Fiscal Year: 2013
    ..aureus infections. ..
  39. Characterizing the role of type IV-secreted CpeD and CpeE in Coxiella burnetii in
    Daniel E Voth; Fiscal Year: 2013
    ..burnetii actively regulates multiple host processes, including vesicular trafficking and cell survival. The bacterial proteins mediating these events are not known but are likely delivered to the host cytosol by a Dot/Icm type IV ..
  40. Development of Novel Topoisomerase and Replication Initiator Assays
    James M Berger; Fiscal Year: 2012
    ..This effort will develop several new assays that monitor key reactions carried out by two essential bacterial proteins in support of DNA replication and cell growth...
  41. Delivery of a bacterial inhibitor of NF-kB to colon tumors
    PHILIP ROSS HARDWIDGE; Fiscal Year: 2012
    ....
  42. Cell Cycle Regulation in Caulobacter Crescentus
    Michael Laub; Fiscal Year: 2013
    ..Finally, by comparing the regulatory strategies unveiled here to those employed in eukaryotes our work will help to reveal the design principles of regulatory circuits throughout biology. ..
  43. Studies of a Bacterial Protein N-Lysine Acetyltransferase
    Sandy Thao; Fiscal Year: 2010
    ..These variant proteins may be critical in the genetic engineering of strains with desirable metabolic capabilities. ..
  44. Cellular Intoxication Pathway of Cytolethal Distending Toxin
    KENNETH ALAN BRADLEY; Fiscal Year: 2013
    ....
  45. A new technology for preclinical development of functional bispecific antibodies
    Lee A Henderson; Fiscal Year: 2010
    ..coli. ..
  46. Role of Adaptive Immunity in the Development of Colitis in TLR5 Deficient Mice
    Matam Vijay Kumar; Fiscal Year: 2013
    ..It will shed light on the crosstalk between innate and adaptive immunity that ultimately culminates in spontaneous colitis. ..
  47. Dynamic cellular architecture of bacteria by system-wide super-resolution imaging
    Xiaowei Zhuang; Fiscal Year: 2013
    ..For most bacterial proteins, we do not know their quantities inside the cell, nor do we know their spatial distributions and ultra-..
  48. Microparticle Capture-Based Analysis of Lymph Node Proteome
    Serguei Popov; Fiscal Year: 2013
    ..The core shell nanoparticles containing different baits demonstrate high-affinity capture of host and bacterial proteins that can be reliably identified by MS and further validated using the highly-sensitive, high-throughput ..
  49. Development of a vaccine for prevention of Haemophilus influenzae otitis media
    Stephen J Barenkamp; Fiscal Year: 2013
    ..We previously identified two related bacterial proteins that are very promising vaccine candidates and we will further assess their vaccine potential in the studies ..
  50. Identification of Bacterial Resistance Mechanisms to Antimicrobial Chemokines
    David L Erickson; Fiscal Year: 2012
    ..application tests the hypothesis that different bacteria are not uniformly affected by AMCs, and identifying bacterial proteins that modulate AMC susceptibility will uncover processes that could provide novel targets for antimicrobial ..
  51. ADHESIN-BASED NANOTHERAPEUTICS IN URINARY TRACT INFECTION
    DAVID ALAN HUNSTAD; Fiscal Year: 2013
    ..We will evaluate the ability of these nanoparticles to prevent and treat UTI. ..
  52. TRANSLOCATION OF DNA ACROSS THE AGROBACTERIUM ENVELOPE
    Peter J Christie; Fiscal Year: 2013
    ..channel subunit: i) it spans the entire Gram-negative cell envelope, a novel property among all described bacterial proteins, ii) it forms an ?-helical pore at the outer membrane, only the second described ?-helical outer membrane ..
  53. A unique mouse model for studying immunity to virulent Franciscella infections
    Gary R Klimpel; Fiscal Year: 2010
    ..Our overall goal is to test the hypothesis that antibody specific for bacterial proteins can be protective for mice following aerosol challenge with virulent Francisella tularensis (SCHU S4) and ..
  54. High Throughput Immunoassay for Flagellin
    Eva King; Fiscal Year: 2013
    ..The research could generate several product lines that are complementary to the company's current products and which will enable the company to expand its product range in new markets. ..
  55. Mechanism of transcript elongation control by RfaH
    Irina Artsimovitch; Fiscal Year: 2013
    ..We will also analyze selected RfaH operons by quantitative RT PCR. ..
  56. SSB PROTEIN/DNA INTERACTIONS
    Timothy M Lohman; Fiscal Year: 2013
    ..Our basic studies of EcoSSB and its interacting proteins can potentially lead to a new target for the development of new antibiotics. ..
  57. Mechanisms of Enteropathogenic E. coli Effects on Polarity and Tight Junctions
    Gail A Hecht; Fiscal Year: 2013
    ..The rationale for the proposed research is that the identification of mechanisms by which pathogenic bacterial proteins hijack host cell regulatory controls to alter physiology will guide the development of novel strategies ..
  58. NF-kB /Beta-catenin interaction during intestinal inflammation
    Jun Sun; Fiscal Year: 2011
    ..This has direct relevance to a better understanding of diseases such as inflammatory bowel diseases and infectious colitis. ..
  59. Analysis of the interplay between cell adhesion and bacterial protein translocati
    Ralph R Isberg; Fiscal Year: 2013
    ..The ability to disrupt collaborative bacterial virulence factor interactions that promote the disease process is critical for identifying strategies that maintain immune function in the presence of pathogen attack. ..
  60. H. pylori-induced inflammation and gastric cancer
    Richard M Peek; Fiscal Year: 2013
    ..pathogenicity island, and several cag genes encode components of a type IV secretion system which exports bacterial proteins such as CagA into host epithelial cells. Our group has now demonstrated that H...
  61. Nitrosative Stress in Bacteria: Molecular Targets and Mechanisms of Resistance
    Jonathan S Stamler; Fiscal Year: 2011
    ..including an endogenous nitrosative stress that we show is responsible for regulating the S-nitrosylation of bacterial proteins. In Aim 2, we examine the enzymatic mechanisms that regulate nitrosative stress through protein ..
  62. Evolution of a bacterial genome encoding increased virulence: the genomics of Hel
    Mark H Forsyth; Fiscal Year: 2012
    ..To examine the evolution of virulence in this significant human pathogen, more research is needed on the genomes of H. pylori strains that produce disease less frequently ..
  63. ANXIETY--GABA RECEPTOR AND OPIOID GENE TRANSFER
    MARLENE WILSON; Fiscal Year: 2000
    ..It will thus help to elucidate the role(s) of specific, localized neurotransmitter functions in complex behaviors such as anxiety. ..
  64. Research and Development of a Novel System to Produce Polysaccharide Conjugate Va
    Peng Wang; Fiscal Year: 2009
    ..This method will firstly be explored with a single pathogen as a model. Then the established method can be easily applied to other pathogens. ..
  65. INTERACTION OF GROUP A STREP WITH THE PLASMINOGEN SYSTEM
    Michael Boyle; Fiscal Year: 2001
    ..as well as a more complicated pathway involving two host proteins (plasminogen and fibrinogen) and two bacterial proteins (the secreted bacterial plasminogen activator, streptokinase (SK), and a surface fibrinogen-binding protein)...
  66. B27 BINDING BACTERIAL AND SELF PEPTIDES IN B27 ARTHRITIS
    R Scofield; Fiscal Year: 2000
    ..demonstrates that this B27 derived peptide can be bound by B27 as are several peptides derived from enteric bacterial proteins. Based on these findings, a new hypothesis for the relationship of spondyloarthropathies to B27 and enteric ..
  67. CMV ACCESSORY GENES AND RETINAL CELL INFECTION
    Lenore Pereira; Fiscal Year: 1999
    ..These herpesvirus and bacterial proteins are also similar to families of mammalian Ca2+ and C1- transporters, which predicts their possible functions...
  68. Borrelia lipoproteins with the cerebral microcirculation
    Diego Cadavid; Fiscal Year: 2007
    ..Various bacterial proteins that contribute to BBB penetration by transcytosis have been identified in gram positive and gram-negative ..
  69. HUMAN N FORMYL PEPTIDE RECEPTOR--REGULATION AND GENETICS
    David Haviland; Fiscal Year: 2000
    ....
  70. Production of Polysaccharide-Protein Conjugates as Vaccines
    Wen Yi; Fiscal Year: 2009
    ..In this program, we develop a novel system to produce conjugate vaccines in a facile and widely applicable manner compared to the traditional chemical approach. ..
  71. Helicobacter Host Interactions in Animal Models
    Stanley Falkow; Fiscal Year: 2009
    ....
  72. THE ROLE OF RETINOIDS IN LACRIMAL GLAND FUNCTION
    John Ubels; Fiscal Year: 2002
    ..The lacrimal gland fluid provides the ocular surface with lubrication, anti-bacterial proteins, electrolytes and nutrients...
  73. TRANSCRIPTIONAL CONTROL OF GASTRIN
    Juanita Merchant; Fiscal Year: 2007
    ..pylori infection, it has now been proposed that gastrin is regulated by bacterial proteins and/or cytokines...
  74. An in vivo Gene Deletion System for Analyzing Temporal Requirement of the Dot/Icm
    Zhao Qing Luo; Fiscal Year: 2009
    ..Legionella pneumophila, the causative agent of Legionnaires'disease transfers a large number of bacterial proteins into host cells via a specialized protein translocation system termed Dot/Icm to construct an intracellular ..
  75. Role of Adapter Protein in Infectious Diseases
    Maria Diakonova; Fiscal Year: 2005
    ..abstract_text> ..