type i antifreeze proteins
Summary: A subclass of ANTIFREEZE PROTEINS that are 3-5 kDa in size and contain a single alanine-rich amphipathic alpha-helix.
- A mechanism for stabilization of membranes at low temperatures by an antifreeze proteinMelanie M Tomczak
Section of Molecular and Cellular Biology, University of California, Davis, California 95616, USA
Biophys J 82:874-81. 2002..The data suggest a hydrophobic interaction between the peptide and the bilayer. Further, we suggest that the expression of AFP type I in transgenic plants may be significant for thermal adaptation of chilling-sensitive plants...
- Solution structure of a hydrophobic analogue of the winter flounder antifreeze proteinEdvards Liepinsh
Karolinska Institute, , Stockholm, Sweden
Eur J Biochem 269:1259-66. 2002..The present study presents the first complete sequence-specific resonance assignments and the first complete solution structure determination by NMR of any AFP I protein...
- Effects of warming rate, temperature, and antifreeze proteins on the survival of mouse spermatozoa frozen at an optimal rateChihiro Koshimoto
Department of Biochemistry and Cellular and Molecular Biology, The University of Tennessee, Knoxville, TN 37392 2575, USA
Cryobiology 45:49-59. 2002..We suggest that such changes may be the basis of the adverse effects of AFP on the survival of the sperm, especially since mouse sperm are exquisitely sensitive to a variety of mechanical stresses...
- Lipid unsaturation determines the interaction of AFP type I with model membranes during thermotropic phase transitionsMelanie M Tomczak
Section of Molecular and Cellular Biology, University of California, Davis, CA 95616, USA
Cryobiology 45:135-42. 2002..The results indicate that the liposomes and protein still interact, even following hydrogenation of the acyl chains, perhaps at the membrane-solution interface...
- Solution structure of a recombinant type I sculpin antifreeze proteinAnn H Y Kwan
School of Chemistry, The University of Sydney, NSW 2006, Australia
Biochemistry 44:1980-8. 2005..These data provide some insight into the structural determinants of antifreeze activity and should assist in the development of models that explain the recognition of different ice interfaces by the sculpin and flounder type I AFPs...
- Type I antifreeze proteins expressed in snailfish skin are identical to their plasma counterpartsRobert P Evans
Ocean Sciences Centre, Memorial University of Newfoundland, St John s, Newfoundland, Canada
FEBS J 272:5327-36. 2005b>Type I antifreeze proteins (AFPs) are usually small, Ala-rich alpha-helical polypeptides found in right-eyed flounders and certain species of sculpin...
- Type I antifreeze proteins: possible origins from chorion and keratin genes in Atlantic snailfishRobert P Evans
Ocean Sciences Centre, Memorial University of Newfoundland, St John s, Newfoundland A1C 5S7, Canada
J Mol Evol 61:417-24. 2005b>Type I antifreeze proteins (AFPs) are alanine-rich alpha-helical polypeptides found in some species of right-eye flounders, sculpin, and snailfish...
- Isolation and characterization of type I antifreeze proteins from Atlantic snailfish (Liparis atlanticus) and dusky snailfish (Liparis gibbus)R P Evans
Ocean Sciences Centre, Memorial University of Newfoundland, A1C 5S7, St John s, NF, Canada
Biochim Biophys Acta 1547:235-44. 2001..Results of this study have indicated that although the AFPs from snailfish are significantly larger than previously described type I AFPs, they share enough characteristics to be classified in this group...
- Structures and ice-binding faces of the alanine-rich type I antifreeze proteinsShruti N Patel
Department of Molecular and Cellular Biology, University of Guelph, Guelph, ON N1G 2W1, Canada
Biochem Cell Biol 88:223-9. 2010..We also propose that the denaturation of hyperactive type I AFP at room temperature is explained by the stabilization of the dimerization interface through hydrogen bonds...
- Insight into the binding of antifreeze proteins to ice surfaces via 13C spin lattice relaxation solid-state NMRYougang Mao
Department of Chemistry and Biochemistry, California State University, Los Angeles, 90032, USA
Biophys J 91:1059-68. 2006The primary sequences of type I antifreeze proteins (AFPs) are Ala rich and contain three 11-residue repeat units beginning with threonine residues. Their secondary structures consist of alpha-helices...
- Applications of type I antifreeze proteins: studies with model membranes & cryoprotectant propertiesSteven R Inglis
School of Chemistry, The University of Sydney, NSW, Australia
Curr Protein Pept Sci 7:509-22. 2006..The major focus will be on the alpha-helical type I antifreeze proteins, and synthetic mutants, that have been most widely studied...
- Hyperactive antifreeze protein in flounder species. The sole freeze protectant in American plaiceSherry Y Gauthier
Department of Biochemistry, Queen s University, Kingston, ON, Canada
FEBS J 272:4439-49. 2005..The previously known, smaller and much less active type I antifreeze proteins cannot by themselves protect the flounder down to the freezing point of seawater...
- Helical antifreeze proteins have independently evolved in fishes on four occasionsLaurie A Graham
Department of Biomedical and Molecular Sciences, Queen s University, Kingston, Ontario, Canada
PLoS ONE 8:e81285. 2013....
- Cryoprotection and cryosterilization effects of type I antifreeze protein on E. coli cellsHidehisa Kawahara
Department of Life Science and Biotechnology, Kansai University, 3 3 35 Yamate cho, Suita, Osaka 564 8680, Japan
Biocontrol Sci 14:49-54. 2009..35% of that using 10 microg/ml under the same conditions. This is the first report on the cryoprotectant and cryosterilization effects of type I AFP of E. coli cells under various conditions...
- Impact of antifreeze proteins and antifreeze glycoproteins on bovine sperm during freeze-thawN S Prathalingam
Royal Veterinary College, Royal College Street, London NW1 0TU, UK
Theriogenology 66:1894-900. 2006..Previous research has shown that osmotic resistance correlates with bull fertility, suggesting that bull spermatozoa cryopreserved in the presence of AFPI may have increased fertility in vivo...
- Isolation and characterization of type I antifreeze proteins from cunner, Tautogolabrus adspersus, order PerciformesRod S Hobbs
Ocean Sciences Centre, Memorial University of Newfoundland, St John s, NL, Canada
FEBS J 278:3699-710. 2011..These results further solidify the hypothesis that type I AFPs are multiphyletic in origin and suggest that they represent remarkable examples of convergent evolution within three orders of teleost fish...
- Type I antifreeze proteins enhance ice nucleation above certain concentrationsPeter W Wilson
School of Life and Environmental Sciences, University of Tsukuba, Ibaraki 305 8572, Japan
J Biol Chem 285:34741-5. 2010..Using an automated lag time apparatus and a new analysis, we show several dilution series of Type I antifreeze proteins. Our results indicate that, above a concentration of ∼8 mg/ml, ice nucleation is enhanced rather than ..
- Antifreeze protein suppresses spontaneous neural activity and protects neurons from hypothermia/re-warming injuryLiel Rubinsky
Department of Anesthesia and Perioperative Care, University of California at San Francisco, 513 Parnassus Avenue, San Francisco, CA 94143 0542, USA
Neurosci Res 67:256-9. 2010..The protection observed is believed to be associated with the inhibitory effect of antifreeze protein...
- Diffusion NMR studies on fish antifreeze proteins and synthetic analoguesSteven R Inglis
School of Chemistry, The University of New South Wales, Sydney, NSW 2052, Australia
FEBS Lett 580:3911-5. 2006..These results are compared with previously reported solubility and thermal hysteresis data and the implications for the design of synthetic antifreeze proteins are discussed...
- Protein digestibility evaluations of meat and fish substrates using laboratory, avian, and ileally cannulated dog assaysT A Faber
Department of Animal Sciences, University of Illinois, Urbana, IL 61801, USA
J Anim Sci 88:1421-32. 2010....
- The antifreeze protein type I (AFP I) increases seabream (Sparus aurata) embryos tolerance to low temperaturesV Robles
CCMAR, Center for Marine Sciences, University of Algarve, Campus de Gambelas, Faro 8005 139, Portugal
Theriogenology 68:284-9. 2007..These results support the hypothesis that AFP protect cellular structures by stabilizing cellular membranes...
- Activity of short segments of Type I antifreeze proteinHagit Kun
Department of Chemistry, Bar Ilan University, Ramat Gan 52900, Israel
Biopolymers 88:807-14. 2007..This approach enables the preparation of large quantities of short AFP segments at low cost with high antifreeze activity, and opens the possibility of developing the commercial potential of AFPs...
- [From molecular genetics to the clinic]C Salpietro
Con la collaborazione di S Briuglia, L Rigoli, C Cuppari
Minerva Pediatr 59:559-61. 2007
- Incorporation of antifreeze proteins into zebrafish embryos by a non-invasive methodS Martínez-Páramo
Department of Molecular Biology, Area of Cell Biology, University of Leon, Campus de Vegazana 24071, Leon, Spain
Cryobiology 56:216-22. 2008..Our findings demonstrate that delivery of AFP type I and AFP type III into zebrafish embryos by incubation in media containing protein is a simple and harmless method that may improve cryoprotection of the cellular compartment...
- Isothermal calorimetry study of the interactions of type I antifreeze proteins with a lipid model membraneHagit Kun
Department of Chemistry, Institute of Nanotechnology Bar Ilan University, Ramat Gan 52900, Israel
Protein Pept Lett 17:739-43. 2010..The heat of interaction above phase transition is assigned to the interaction of the AFP with the membrane, while below phase transition the ordering effect of the AFP influence the heat of interaction...
- Functional secretion of a type 1 antifreeze protein analogue by optimization of promoter, signal peptide, prosequence, and terminator in Lactococcus lactisChuan Mei Yeh
Department of Food Science and Biotechnology, National Chung Hsing University, Taichung, Taiwan, Republic of China
J Agric Food Chem 56:8442-50. 2008..The extracellularly expressed rAFP successfully inhibited ice recrystallization and is thus potentially applicable for cryogenic preservation...
- Cooperative function of ammonium polyacrylate with antifreeze protein type IKunio Funakoshi
National Institute of Advanced Industrial Science and Technology, 1 2 1 Namiki, Tsukuba, Ibaraki 305 8564, Japan
Biomacromolecules 9:3150-6. 2008..The experimental results suggest that the cooperative function of NH4PA with AFP type I was caused either by the increase in adsorption sites of AFP type I on ice or by the adsorption of AFP type I aggregates on ice...
- Focus on urinary bladder cancer markers: a reviewT H Ecke
HELIOS Hospital, Department of Urology, Bad Saarow, Germany
Minerva Urol Nefrol 60:237-46. 2008..Molecular staging of urological tumors will allow selecting cases that will require systemic treatment. It is necessary and important to integrate under the same objectives basic and clinical research...
- Production of a recombinant type 1 antifreeze protein analogue by L. lactis and its applications on frozen meat and frozen doughChuan Mei Yeh
Department of Food Science and Biotechnology, National Chung Hsing University, Taichung, Taiwan, Republic of China
J Agric Food Chem 57:6216-23. 2009..Frozen dough treated with the rAFP showed better fermentation capacity than untreated frozen dough. Breads baked from frozen dough treated with rAFP acquired the same consumer acceptance as fresh bread...
- Antifreeze protein gene expression in winter flounder pre-hatch embryos: implications for cryopreservationHeather M Young
Ocean Sciences Centre and Department of Biology, Memorial University of Newfoundland, St John s, NL, Canada A1C5S7
Cryobiology 57:84-90. 2008..Neither of the liver type AFP genes was expressed in the embryos...
- Mediated modeling of the impacts of enhanced UV-B radiation on ecosystem servicesMarjan van den Belt
Mediated Modeling Partners LLC, Charlotte, VT, USA
Photochem Photobiol 82:865-77. 2006..In summary, although changes in the marine sector due to anthropogenic influences may affect global climate change, marshes are expected to primarily be affected by climate change...
- Effect of antifreeze proteins on the nucleation, growth, and the memory effect during tetrahydrofuran clathrate hydrate formationHuang Zeng
Department of Biology, Queen s University, Kingston, Ontario K7L 3N6, Canada
J Am Chem Soc 128:2844-50. 2006..On the basis of our results, we postulate a mechanism for heterogeneous nucleation, the memory effect and its elimination by antifreeze proteins...
- The dual effect of antifreeze protein on cryopreservation of rice (Oryza sativa l.) embryogenic suspension cellsJ H Wang
College of Life Sciences, Zhejiang University, Hangzhou 310012, China
Cryo Letters 22:175-82. 2001..The possible mechanism of this dual character was discussed...
- Structure of type I antifreeze protein and mutants in supercooled waterS P Graether
CIHR Group in Protein Structure and Function, Department of Biochemistry, University of Alberta, Edmonton, Alberta, T6G 2H7, Canada
Biophys J 81:1677-83. 2001..These data have important implications for the structure of the AFP bound to ice as well as the mechanisms for ice-binding and protein oligomerization...
- Successful nonfreezing, subzero preservation of rat liver with 2,3-butanediol and type I antifreeze proteinK A Soltys
Department of Surgery, Transplant Research Laboratory, University of Medicine and Dentistry New Jersey Medical School, 185 South Orange Avenue, Newark, New Jersey 07103, USA
J Surg Res 96:30-4. 2001..Although as effective as current +4 degrees C protocols, subzero preservation for longer periods needs to be achieved prior to clinical application...
- Type I 'antifreeze' proteins. Structure-activity studies and mechanisms of ice growth inhibitionM M Harding
School of Chemistry, University of Sydney, NSW 2006, Australia
Eur J Biochem 264:653-65. 1999....
- Skin-type antifreeze protein from the shorthorn sculpin, Myoxocephalus scorpius. Expression and characterization of a Mr 9, 700 recombinant proteinW K Low
Structural Biology and Biochemistry Division, Hospital for Sick Children, and Departments of Biochemistry and Laboratory Medicine and Pathobiology, University of Toronto, Toronto M5G 1L5, Ontario, Canada
J Biol Chem 273:23098-103. 1998..These studies reinforce recent evidence that indicate that the external tissues of cold water marine fishes are major organs for antifreeze protein synthesis and are likely the first line of defense against the threat of freezing...
- Reversible binding of the HPLC6 isoform of type I antifreeze proteins to ice surfaces and the antifreeze mechanism studied by multiple quantum filtering-spin exchange NMR experimentYong Ba
Department of Chemistry and Biochemistry, California State University Los Angeles, 5151 State University Drive, 90032, USA
J Am Chem Soc 125:330-1. 2003..This concentration gradient creates a dense layer of AFP in contact with the ice-binding surface, which depresses the local freezing point because of the colligative property, but not the Kelvin effect...
- Freezing of a fish antifreeze protein results in amyloid fibril formationSteffen P Graether
Canadian Institute of Health Research Group in Protein Structure and Function, Department of Biochemistry and Protein Engineering Network of Centres of Excellence, University of Alberta, Edmonton, T6G 2H7 Canada
Biophys J 84:552-7. 2003..Our results demonstrate that the freezing of some proteins may accelerate the formation of amyloid fibrils...
- Heterologous expression of type I antifreeze peptide GS-5 in baker's yeast increases freeze tolerance and provides enhanced gas production in frozen doughJoaquin Panadero
Department of Biotechnology, Instituto de Agroquimica y Tecnologia de los Alimentos, Consejo Superior de Investigaciones Cientificas, P O Box 73, 46100 Burjassot Valencia, Spain
J Agric Food Chem 53:9966-70. 2005..These effects are unlikely to be due to reduced osmotic damage during freezing/thawing, because recombinant cells showed growth behavior similar to that of the parent under hypermosmotic stress conditions...
- Hydrophobic tendency of polar group hydration as a major force in type I antifreeze protein recognitionCheng Yang
E R Johnson Research Foundation, Department of Biochemistry and Biophysics, University of Pennsylvania, Philadelphia, Pennsylvania 19104 6049, USA
Proteins 59:266-74. 2005..The specific shape, residue size, and clustering of both polar and apoler groups are essential for an active ice binding surface...
- Hyperactive antifreeze protein from winter flounder is a very long rod-like dimer of alpha-helicesChristopher B Marshall
Department of Biochemistry, Queen s University, Kingston, Ontario, Canada
J Biol Chem 280:17920-9. 2005....
- [A novel antifreeze protein in fish in the North Atlantic]Christopher B Marshall
Med Sci (Paris) 20:746-7. 2004
- Subzero nonfreezing cryopresevation of rat hearts using antifreeze protein I and antifreeze protein IIIGabriel Amir
Department of Cardiac Surgery, Sheba Medical Center, Tel Hashomer, Israel
Cryobiology 48:273-82. 2004..4 vs. 1.7, p<0.05, and 3.4 vs. 1.7, p<0.05, respectively). In conclusion, AFPs were found to protect the heart from freezing and improve survival and dP (AFP III) in prolonged subzero preservation...
- Hyperactive antifreeze protein in a fishChristopher B Marshall
Department of Biochemistry, Queen s University, Kingston, Ontario K7L 3N6, Canada
Nature 429:153. 2004..Here we describe a previously undiscovered antifreeze protein from this flounder that is extremely active (as effective as those found in insects) and which explains the resistance of this fish to freezing in polar and subpolar waters...
- The effect of hydrophobic analogues of the type I winter flounder antifreeze protein on lipid bilayersMelanie M Tomczak
Division of Biological Sciences, University of California, Davis, CA 95616, USA
FEBS Lett 551:13-9. 2003..The different effects correlate with the helicity of the peptides and suggest that the solution conformation of the peptides has a significant role in determining the effects of the peptides on thermotropic membrane phase transitions...
- Spatial expression patterns of skin-type antifreeze protein in winter flounder (Pseudopleuronectes americanus) epidermis following metamorphosisHarry M Murray
Ocean Sciences Centre, Memorial University of Newfoundland, St John s, Newfoundland, Canada A1C 5S7
J Morphol 257:78-86. 2003..This distribution suggests that the AFP may be important in slowing ice crystal formation in these interstitial regions and thus reducing cellular damage due to osmotic imbalance...
- cDNA sequence analysis and expression of two cold-regulated genes of Arabidopsis thalianaS J Gilmour
Department of Crop and Soil Sciences, Michigan State University, East Lansing 48824
Plant Mol Biol 18:13-21. 1992..The similarities and differences between COR and LEA proteins are discussed as are their possible roles in freezing and drought tolerance...