type i antifreeze proteins

Summary

Summary: A subclass of ANTIFREEZE PROTEINS that are 3-5 kDa in size and contain a single alanine-rich amphipathic alpha-helix.

Top Publications

  1. pmc A mechanism for stabilization of membranes at low temperatures by an antifreeze protein
    Melanie M Tomczak
    Section of Molecular and Cellular Biology, University of California, Davis, California 95616, USA
    Biophys J 82:874-81. 2002
  2. ncbi Solution structure of a hydrophobic analogue of the winter flounder antifreeze protein
    Edvards Liepinsh
    Karolinska Institute, , Stockholm, Sweden
    Eur J Biochem 269:1259-66. 2002
  3. ncbi Effects of warming rate, temperature, and antifreeze proteins on the survival of mouse spermatozoa frozen at an optimal rate
    Chihiro Koshimoto
    Department of Biochemistry and Cellular and Molecular Biology, The University of Tennessee, Knoxville, TN 37392 2575, USA
    Cryobiology 45:49-59. 2002
  4. ncbi Lipid unsaturation determines the interaction of AFP type I with model membranes during thermotropic phase transitions
    Melanie M Tomczak
    Section of Molecular and Cellular Biology, University of California, Davis, CA 95616, USA
    Cryobiology 45:135-42. 2002
  5. ncbi Solution structure of a recombinant type I sculpin antifreeze protein
    Ann H Y Kwan
    School of Chemistry, The University of Sydney, NSW 2006, Australia
    Biochemistry 44:1980-8. 2005
  6. ncbi Type I antifreeze proteins expressed in snailfish skin are identical to their plasma counterparts
    Robert P Evans
    Ocean Sciences Centre, Memorial University of Newfoundland, St John s, Newfoundland, Canada
    FEBS J 272:5327-36. 2005
  7. ncbi Type I antifreeze proteins: possible origins from chorion and keratin genes in Atlantic snailfish
    Robert P Evans
    Ocean Sciences Centre, Memorial University of Newfoundland, St John s, Newfoundland A1C 5S7, Canada
    J Mol Evol 61:417-24. 2005
  8. ncbi Isolation and characterization of type I antifreeze proteins from Atlantic snailfish (Liparis atlanticus) and dusky snailfish (Liparis gibbus)
    R P Evans
    Ocean Sciences Centre, Memorial University of Newfoundland, A1C 5S7, St John s, NF, Canada
    Biochim Biophys Acta 1547:235-44. 2001
  9. ncbi Structures and ice-binding faces of the alanine-rich type I antifreeze proteins
    Shruti N Patel
    Department of Molecular and Cellular Biology, University of Guelph, Guelph, ON N1G 2W1, Canada
    Biochem Cell Biol 88:223-9. 2010
  10. pmc Insight into the binding of antifreeze proteins to ice surfaces via 13C spin lattice relaxation solid-state NMR
    Yougang Mao
    Department of Chemistry and Biochemistry, California State University, Los Angeles, 90032, USA
    Biophys J 91:1059-68. 2006

Scientific Experts

  • Robert P Evans
  • Chuan Mei Yeh
  • M M Tomczak
  • Harry M Murray
  • J H Wang
  • T D Ecke
  • Nilendran S Prathalingam
  • V Robles
  • Yong Ba
  • M M Harding
  • Gabriel Amir
  • Peter W Wilson
  • Liel Rubinsky
  • Peter L Davies
  • Christopher B Marshall
  • Garth L Fletcher
  • Hagit Kun
  • Laurie A Graham
  • Rod S Hobbs
  • Steven R Inglis
  • Steffen P Graether
  • Shruti N Patel
  • T A Faber
  • Hidehisa Kawahara
  • Yitzhak Mastai
  • S Martínez-Páramo
  • Heather M Young
  • Kunio Funakoshi
  • C Salpietro
  • Huang Zeng
  • Yougang Mao
  • Marjan van den Belt
  • Cheng Yang
  • Ann H Y Kwan
  • Joaquin Panadero
  • Sherry Y Gauthier
  • Joel P Mackay
  • Chihiro Koshimoto
  • Edvards Liepinsh
  • S P Graether
  • K A Soltys
  • Margaret A Shears
  • G C Fahey
  • K S Swanson
  • P J Bechtel
  • C M Parsons
  • S Smiley
  • D C Hernot
  • Hitoshi Obata
  • Hiroyuki Tatsukawa
  • Sakura Higa
  • Takaaki Inada
  • S Pérez-Cerezales
  • Hiroshi Kawabata
  • M P Herraez
  • Takashi Tomita
  • L Anel
  • Susana Diaz
  • Jennifer J Turner
  • Serge Demers
  • Lee D Wilson
  • W K Low
  • Virginia K Walker
  • Gustavo A Ferreyra
  • Robert Costanza
  • Matthew J McGann
  • Oscar A Bianciotto
  • William S Price
  • John A Ripmeester
  • Maria Vernet
  • Fernando R Momo
  • Evamaria W Koch
  • Pia I Anderberg
  • Kayesh Fairley
  • Chu Wai Liew
  • Francisca Randez-Gil
  • Avijit Chakrabartty
  • Jose Antonio Prieto
  • Kim A Sharp
  • Nathalie Dourdin
  • Dominic Cuerrier
  • Brian D Sykes
  • Carolyn M Slupsky
  • Gottfried Otting
  • Peter Mazur
  • Leanne G Ward
  • A D J Haymet
  • C M Slupsky
  • P L Davies
  • B D Sykes

Detail Information

Publications48

  1. pmc A mechanism for stabilization of membranes at low temperatures by an antifreeze protein
    Melanie M Tomczak
    Section of Molecular and Cellular Biology, University of California, Davis, California 95616, USA
    Biophys J 82:874-81. 2002
    ..The data suggest a hydrophobic interaction between the peptide and the bilayer. Further, we suggest that the expression of AFP type I in transgenic plants may be significant for thermal adaptation of chilling-sensitive plants...
  2. ncbi Solution structure of a hydrophobic analogue of the winter flounder antifreeze protein
    Edvards Liepinsh
    Karolinska Institute, , Stockholm, Sweden
    Eur J Biochem 269:1259-66. 2002
    ..The present study presents the first complete sequence-specific resonance assignments and the first complete solution structure determination by NMR of any AFP I protein...
  3. ncbi Effects of warming rate, temperature, and antifreeze proteins on the survival of mouse spermatozoa frozen at an optimal rate
    Chihiro Koshimoto
    Department of Biochemistry and Cellular and Molecular Biology, The University of Tennessee, Knoxville, TN 37392 2575, USA
    Cryobiology 45:49-59. 2002
    ..We suggest that such changes may be the basis of the adverse effects of AFP on the survival of the sperm, especially since mouse sperm are exquisitely sensitive to a variety of mechanical stresses...
  4. ncbi Lipid unsaturation determines the interaction of AFP type I with model membranes during thermotropic phase transitions
    Melanie M Tomczak
    Section of Molecular and Cellular Biology, University of California, Davis, CA 95616, USA
    Cryobiology 45:135-42. 2002
    ..The results indicate that the liposomes and protein still interact, even following hydrogenation of the acyl chains, perhaps at the membrane-solution interface...
  5. ncbi Solution structure of a recombinant type I sculpin antifreeze protein
    Ann H Y Kwan
    School of Chemistry, The University of Sydney, NSW 2006, Australia
    Biochemistry 44:1980-8. 2005
    ..These data provide some insight into the structural determinants of antifreeze activity and should assist in the development of models that explain the recognition of different ice interfaces by the sculpin and flounder type I AFPs...
  6. ncbi Type I antifreeze proteins expressed in snailfish skin are identical to their plasma counterparts
    Robert P Evans
    Ocean Sciences Centre, Memorial University of Newfoundland, St John s, Newfoundland, Canada
    FEBS J 272:5327-36. 2005
    b>Type I antifreeze proteins (AFPs) are usually small, Ala-rich alpha-helical polypeptides found in right-eyed flounders and certain species of sculpin...
  7. ncbi Type I antifreeze proteins: possible origins from chorion and keratin genes in Atlantic snailfish
    Robert P Evans
    Ocean Sciences Centre, Memorial University of Newfoundland, St John s, Newfoundland A1C 5S7, Canada
    J Mol Evol 61:417-24. 2005
    b>Type I antifreeze proteins (AFPs) are alanine-rich alpha-helical polypeptides found in some species of right-eye flounders, sculpin, and snailfish...
  8. ncbi Isolation and characterization of type I antifreeze proteins from Atlantic snailfish (Liparis atlanticus) and dusky snailfish (Liparis gibbus)
    R P Evans
    Ocean Sciences Centre, Memorial University of Newfoundland, A1C 5S7, St John s, NF, Canada
    Biochim Biophys Acta 1547:235-44. 2001
    ..Results of this study have indicated that although the AFPs from snailfish are significantly larger than previously described type I AFPs, they share enough characteristics to be classified in this group...
  9. ncbi Structures and ice-binding faces of the alanine-rich type I antifreeze proteins
    Shruti N Patel
    Department of Molecular and Cellular Biology, University of Guelph, Guelph, ON N1G 2W1, Canada
    Biochem Cell Biol 88:223-9. 2010
    ..We also propose that the denaturation of hyperactive type I AFP at room temperature is explained by the stabilization of the dimerization interface through hydrogen bonds...
  10. pmc Insight into the binding of antifreeze proteins to ice surfaces via 13C spin lattice relaxation solid-state NMR
    Yougang Mao
    Department of Chemistry and Biochemistry, California State University, Los Angeles, 90032, USA
    Biophys J 91:1059-68. 2006
    The primary sequences of type I antifreeze proteins (AFPs) are Ala rich and contain three 11-residue repeat units beginning with threonine residues. Their secondary structures consist of alpha-helices...
  11. ncbi Applications of type I antifreeze proteins: studies with model membranes & cryoprotectant properties
    Steven R Inglis
    School of Chemistry, The University of Sydney, NSW, Australia
    Curr Protein Pept Sci 7:509-22. 2006
    ..The major focus will be on the alpha-helical type I antifreeze proteins, and synthetic mutants, that have been most widely studied...
  12. ncbi Hyperactive antifreeze protein in flounder species. The sole freeze protectant in American plaice
    Sherry Y Gauthier
    Department of Biochemistry, Queen s University, Kingston, ON, Canada
    FEBS J 272:4439-49. 2005
    ..The previously known, smaller and much less active type I antifreeze proteins cannot by themselves protect the flounder down to the freezing point of seawater...
  13. pmc Helical antifreeze proteins have independently evolved in fishes on four occasions
    Laurie A Graham
    Department of Biomedical and Molecular Sciences, Queen s University, Kingston, Ontario, Canada
    PLoS ONE 8:e81285. 2013
    ....
  14. ncbi Cryoprotection and cryosterilization effects of type I antifreeze protein on E. coli cells
    Hidehisa Kawahara
    Department of Life Science and Biotechnology, Kansai University, 3 3 35 Yamate cho, Suita, Osaka 564 8680, Japan
    Biocontrol Sci 14:49-54. 2009
    ..35% of that using 10 microg/ml under the same conditions. This is the first report on the cryoprotectant and cryosterilization effects of type I AFP of E. coli cells under various conditions...
  15. ncbi Impact of antifreeze proteins and antifreeze glycoproteins on bovine sperm during freeze-thaw
    N S Prathalingam
    Royal Veterinary College, Royal College Street, London NW1 0TU, UK
    Theriogenology 66:1894-900. 2006
    ..Previous research has shown that osmotic resistance correlates with bull fertility, suggesting that bull spermatozoa cryopreserved in the presence of AFPI may have increased fertility in vivo...
  16. ncbi Isolation and characterization of type I antifreeze proteins from cunner, Tautogolabrus adspersus, order Perciformes
    Rod S Hobbs
    Ocean Sciences Centre, Memorial University of Newfoundland, St John s, NL, Canada
    FEBS J 278:3699-710. 2011
    ..These results further solidify the hypothesis that type I AFPs are multiphyletic in origin and suggest that they represent remarkable examples of convergent evolution within three orders of teleost fish...
  17. pmc Type I antifreeze proteins enhance ice nucleation above certain concentrations
    Peter W Wilson
    School of Life and Environmental Sciences, University of Tsukuba, Ibaraki 305 8572, Japan
    J Biol Chem 285:34741-5. 2010
    ..Using an automated lag time apparatus and a new analysis, we show several dilution series of Type I antifreeze proteins. Our results indicate that, above a concentration of ∼8 mg/ml, ice nucleation is enhanced rather than ..
  18. ncbi Antifreeze protein suppresses spontaneous neural activity and protects neurons from hypothermia/re-warming injury
    Liel Rubinsky
    Department of Anesthesia and Perioperative Care, University of California at San Francisco, 513 Parnassus Avenue, San Francisco, CA 94143 0542, USA
    Neurosci Res 67:256-9. 2010
    ..The protection observed is believed to be associated with the inhibitory effect of antifreeze protein...
  19. ncbi Diffusion NMR studies on fish antifreeze proteins and synthetic analogues
    Steven R Inglis
    School of Chemistry, The University of New South Wales, Sydney, NSW 2052, Australia
    FEBS Lett 580:3911-5. 2006
    ..These results are compared with previously reported solubility and thermal hysteresis data and the implications for the design of synthetic antifreeze proteins are discussed...
  20. ncbi Protein digestibility evaluations of meat and fish substrates using laboratory, avian, and ileally cannulated dog assays
    T A Faber
    Department of Animal Sciences, University of Illinois, Urbana, IL 61801, USA
    J Anim Sci 88:1421-32. 2010
    ....
  21. ncbi The antifreeze protein type I (AFP I) increases seabream (Sparus aurata) embryos tolerance to low temperatures
    V Robles
    CCMAR, Center for Marine Sciences, University of Algarve, Campus de Gambelas, Faro 8005 139, Portugal
    Theriogenology 68:284-9. 2007
    ..These results support the hypothesis that AFP protect cellular structures by stabilizing cellular membranes...
  22. ncbi Activity of short segments of Type I antifreeze protein
    Hagit Kun
    Department of Chemistry, Bar Ilan University, Ramat Gan 52900, Israel
    Biopolymers 88:807-14. 2007
    ..This approach enables the preparation of large quantities of short AFP segments at low cost with high antifreeze activity, and opens the possibility of developing the commercial potential of AFPs...
  23. ncbi [From molecular genetics to the clinic]
    C Salpietro
    Con la collaborazione di S Briuglia, L Rigoli, C Cuppari
    Minerva Pediatr 59:559-61. 2007
  24. ncbi Incorporation of antifreeze proteins into zebrafish embryos by a non-invasive method
    S Martínez-Páramo
    Department of Molecular Biology, Area of Cell Biology, University of Leon, Campus de Vegazana 24071, Leon, Spain
    Cryobiology 56:216-22. 2008
    ..Our findings demonstrate that delivery of AFP type I and AFP type III into zebrafish embryos by incubation in media containing protein is a simple and harmless method that may improve cryoprotection of the cellular compartment...
  25. ncbi Isothermal calorimetry study of the interactions of type I antifreeze proteins with a lipid model membrane
    Hagit Kun
    Department of Chemistry, Institute of Nanotechnology Bar Ilan University, Ramat Gan 52900, Israel
    Protein Pept Lett 17:739-43. 2010
    ..The heat of interaction above phase transition is assigned to the interaction of the AFP with the membrane, while below phase transition the ordering effect of the AFP influence the heat of interaction...
  26. ncbi Functional secretion of a type 1 antifreeze protein analogue by optimization of promoter, signal peptide, prosequence, and terminator in Lactococcus lactis
    Chuan Mei Yeh
    Department of Food Science and Biotechnology, National Chung Hsing University, Taichung, Taiwan, Republic of China
    J Agric Food Chem 56:8442-50. 2008
    ..The extracellularly expressed rAFP successfully inhibited ice recrystallization and is thus potentially applicable for cryogenic preservation...
  27. ncbi Cooperative function of ammonium polyacrylate with antifreeze protein type I
    Kunio Funakoshi
    National Institute of Advanced Industrial Science and Technology, 1 2 1 Namiki, Tsukuba, Ibaraki 305 8564, Japan
    Biomacromolecules 9:3150-6. 2008
    ..The experimental results suggest that the cooperative function of NH4PA with AFP type I was caused either by the increase in adsorption sites of AFP type I on ice or by the adsorption of AFP type I aggregates on ice...
  28. ncbi Focus on urinary bladder cancer markers: a review
    T H Ecke
    HELIOS Hospital, Department of Urology, Bad Saarow, Germany
    Minerva Urol Nefrol 60:237-46. 2008
    ..Molecular staging of urological tumors will allow selecting cases that will require systemic treatment. It is necessary and important to integrate under the same objectives basic and clinical research...
  29. ncbi Production of a recombinant type 1 antifreeze protein analogue by L. lactis and its applications on frozen meat and frozen dough
    Chuan Mei Yeh
    Department of Food Science and Biotechnology, National Chung Hsing University, Taichung, Taiwan, Republic of China
    J Agric Food Chem 57:6216-23. 2009
    ..Frozen dough treated with the rAFP showed better fermentation capacity than untreated frozen dough. Breads baked from frozen dough treated with rAFP acquired the same consumer acceptance as fresh bread...
  30. ncbi Antifreeze protein gene expression in winter flounder pre-hatch embryos: implications for cryopreservation
    Heather M Young
    Ocean Sciences Centre and Department of Biology, Memorial University of Newfoundland, St John s, NL, Canada A1C5S7
    Cryobiology 57:84-90. 2008
    ..Neither of the liver type AFP genes was expressed in the embryos...
  31. ncbi Mediated modeling of the impacts of enhanced UV-B radiation on ecosystem services
    Marjan van den Belt
    Mediated Modeling Partners LLC, Charlotte, VT, USA
    Photochem Photobiol 82:865-77. 2006
    ..In summary, although changes in the marine sector due to anthropogenic influences may affect global climate change, marshes are expected to primarily be affected by climate change...
  32. ncbi Effect of antifreeze proteins on the nucleation, growth, and the memory effect during tetrahydrofuran clathrate hydrate formation
    Huang Zeng
    Department of Biology, Queen s University, Kingston, Ontario K7L 3N6, Canada
    J Am Chem Soc 128:2844-50. 2006
    ..On the basis of our results, we postulate a mechanism for heterogeneous nucleation, the memory effect and its elimination by antifreeze proteins...
  33. ncbi The dual effect of antifreeze protein on cryopreservation of rice (Oryza sativa l.) embryogenic suspension cells
    J H Wang
    College of Life Sciences, Zhejiang University, Hangzhou 310012, China
    Cryo Letters 22:175-82. 2001
    ..The possible mechanism of this dual character was discussed...
  34. pmc Structure of type I antifreeze protein and mutants in supercooled water
    S P Graether
    CIHR Group in Protein Structure and Function, Department of Biochemistry, University of Alberta, Edmonton, Alberta, T6G 2H7, Canada
    Biophys J 81:1677-83. 2001
    ..These data have important implications for the structure of the AFP bound to ice as well as the mechanisms for ice-binding and protein oligomerization...
  35. ncbi Successful nonfreezing, subzero preservation of rat liver with 2,3-butanediol and type I antifreeze protein
    K A Soltys
    Department of Surgery, Transplant Research Laboratory, University of Medicine and Dentistry New Jersey Medical School, 185 South Orange Avenue, Newark, New Jersey 07103, USA
    J Surg Res 96:30-4. 2001
    ..Although as effective as current +4 degrees C protocols, subzero preservation for longer periods needs to be achieved prior to clinical application...
  36. ncbi Type I 'antifreeze' proteins. Structure-activity studies and mechanisms of ice growth inhibition
    M M Harding
    School of Chemistry, University of Sydney, NSW 2006, Australia
    Eur J Biochem 264:653-65. 1999
    ....
  37. ncbi Skin-type antifreeze protein from the shorthorn sculpin, Myoxocephalus scorpius. Expression and characterization of a Mr 9, 700 recombinant protein
    W K Low
    Structural Biology and Biochemistry Division, Hospital for Sick Children, and Departments of Biochemistry and Laboratory Medicine and Pathobiology, University of Toronto, Toronto M5G 1L5, Ontario, Canada
    J Biol Chem 273:23098-103. 1998
    ..These studies reinforce recent evidence that indicate that the external tissues of cold water marine fishes are major organs for antifreeze protein synthesis and are likely the first line of defense against the threat of freezing...
  38. ncbi Reversible binding of the HPLC6 isoform of type I antifreeze proteins to ice surfaces and the antifreeze mechanism studied by multiple quantum filtering-spin exchange NMR experiment
    Yong Ba
    Department of Chemistry and Biochemistry, California State University Los Angeles, 5151 State University Drive, 90032, USA
    J Am Chem Soc 125:330-1. 2003
    ..This concentration gradient creates a dense layer of AFP in contact with the ice-binding surface, which depresses the local freezing point because of the colligative property, but not the Kelvin effect...
  39. pmc Freezing of a fish antifreeze protein results in amyloid fibril formation
    Steffen P Graether
    Canadian Institute of Health Research Group in Protein Structure and Function, Department of Biochemistry and Protein Engineering Network of Centres of Excellence, University of Alberta, Edmonton, T6G 2H7 Canada
    Biophys J 84:552-7. 2003
    ..Our results demonstrate that the freezing of some proteins may accelerate the formation of amyloid fibrils...
  40. ncbi Heterologous expression of type I antifreeze peptide GS-5 in baker's yeast increases freeze tolerance and provides enhanced gas production in frozen dough
    Joaquin Panadero
    Department of Biotechnology, Instituto de Agroquimica y Tecnologia de los Alimentos, Consejo Superior de Investigaciones Cientificas, P O Box 73, 46100 Burjassot Valencia, Spain
    J Agric Food Chem 53:9966-70. 2005
    ..These effects are unlikely to be due to reduced osmotic damage during freezing/thawing, because recombinant cells showed growth behavior similar to that of the parent under hypermosmotic stress conditions...
  41. ncbi Hydrophobic tendency of polar group hydration as a major force in type I antifreeze protein recognition
    Cheng Yang
    E R Johnson Research Foundation, Department of Biochemistry and Biophysics, University of Pennsylvania, Philadelphia, Pennsylvania 19104 6049, USA
    Proteins 59:266-74. 2005
    ..The specific shape, residue size, and clustering of both polar and apoler groups are essential for an active ice binding surface...
  42. ncbi Hyperactive antifreeze protein from winter flounder is a very long rod-like dimer of alpha-helices
    Christopher B Marshall
    Department of Biochemistry, Queen s University, Kingston, Ontario, Canada
    J Biol Chem 280:17920-9. 2005
    ....
  43. ncbi [A novel antifreeze protein in fish in the North Atlantic]
    Christopher B Marshall
    Med Sci (Paris) 20:746-7. 2004
  44. ncbi Subzero nonfreezing cryopresevation of rat hearts using antifreeze protein I and antifreeze protein III
    Gabriel Amir
    Department of Cardiac Surgery, Sheba Medical Center, Tel Hashomer, Israel
    Cryobiology 48:273-82. 2004
    ..4 vs. 1.7, p<0.05, and 3.4 vs. 1.7, p<0.05, respectively). In conclusion, AFPs were found to protect the heart from freezing and improve survival and dP (AFP III) in prolonged subzero preservation...
  45. ncbi Hyperactive antifreeze protein in a fish
    Christopher B Marshall
    Department of Biochemistry, Queen s University, Kingston, Ontario K7L 3N6, Canada
    Nature 429:153. 2004
    ..Here we describe a previously undiscovered antifreeze protein from this flounder that is extremely active (as effective as those found in insects) and which explains the resistance of this fish to freezing in polar and subpolar waters...
  46. ncbi The effect of hydrophobic analogues of the type I winter flounder antifreeze protein on lipid bilayers
    Melanie M Tomczak
    Division of Biological Sciences, University of California, Davis, CA 95616, USA
    FEBS Lett 551:13-9. 2003
    ..The different effects correlate with the helicity of the peptides and suggest that the solution conformation of the peptides has a significant role in determining the effects of the peptides on thermotropic membrane phase transitions...
  47. ncbi Spatial expression patterns of skin-type antifreeze protein in winter flounder (Pseudopleuronectes americanus) epidermis following metamorphosis
    Harry M Murray
    Ocean Sciences Centre, Memorial University of Newfoundland, St John s, Newfoundland, Canada A1C 5S7
    J Morphol 257:78-86. 2003
    ..This distribution suggests that the AFP may be important in slowing ice crystal formation in these interstitial regions and thus reducing cellular damage due to osmotic imbalance...
  48. ncbi cDNA sequence analysis and expression of two cold-regulated genes of Arabidopsis thaliana
    S J Gilmour
    Department of Crop and Soil Sciences, Michigan State University, East Lansing 48824
    Plant Mol Biol 18:13-21. 1992
    ..The similarities and differences between COR and LEA proteins are discussed as are their possible roles in freezing and drought tolerance...