peptide library

Summary

Summary: A collection of cloned peptides, or chemically synthesized peptides, frequently consisting of all possible combinations of amino acids making up an n-amino acid peptide.

Top Publications

  1. ncbi Progress in phage display: evolution of the technique and its application
    Tomaz Bratkovic
    Department of Pharmaceutical Biology, Faculty of Pharmacy, University of Ljubljana, Askerceva 7, 1000 Ljubljana, Slovenia
    Cell Mol Life Sci 67:749-67. 2010
  2. pmc Structural and functional bases for broad-spectrum neutralization of avian and human influenza A viruses
    Jianhua Sui
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, Harvard Medical School, 44 Binney Street JFB 826, Boston, Massachusetts 02115, USA
    Nat Struct Mol Biol 16:265-73. 2009
  3. ncbi Yeast surface display for screening combinatorial polypeptide libraries
    E T Boder
    Department of Chemical Engineering, University of Illinois, Urbana 61801, USA
    Nat Biotechnol 15:553-7. 1997
  4. ncbi Autoantibody signatures in prostate cancer
    Xiaoju Wang
    Department of Pathology, University of Michigan Medical School, Ann Arbor, MI 48109, USA
    N Engl J Med 353:1224-35. 2005
  5. ncbi The SPOT-synthesis technique. Synthetic peptide arrays on membrane supports--principles and applications
    Ronald Frank
    AG Molecular Recognition, German Research Centre for Biotechnology GBF, Mascheroder Weg 1, D 38124 Brunswick, Germany
    J Immunol Methods 267:13-26. 2002
  6. pmc A compact phage display human scFv library for selection of antibodies to a wide variety of antigens
    Potjamas Pansri
    School of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand
    BMC Biotechnol 9:6. 2009
  7. ncbi DARPins and other repeat protein scaffolds: advances in engineering and applications
    Ykelien L Boersma
    Department of Biochemistry, University of Zurich, Winterthurerstrasse 190, 8057 Zurich, Switzerland
    Curr Opin Biotechnol 22:849-57. 2011
  8. ncbi Rotamer libraries in the 21st century
    Roland L Dunbrack
    Institute for Cancer Research, Fox Chase Cancer Center, 7701 Burholme Avenue, Philadelphia PA 19111, USA
    Curr Opin Struct Biol 12:431-40. 2002
  9. pmc Bayesian modeling of the yeast SH3 domain interactome predicts spatiotemporal dynamics of endocytosis proteins
    Raffi Tonikian
    Terrence Donnelly Center for Cellular and Biomolecular Research, Banting and Best Department of Medical Research, University of Toronto, Toronto, Ontario, Canada
    PLoS Biol 7:e1000218. 2009
  10. ncbi Biophysical properties of human antibody variable domains
    Stefan Ewert
    Biochemisches Institut, Universitat Zurich, Winterthurerstr 190, CH 8057 Zurich, Switzerland
    J Mol Biol 325:531-53. 2003

Detail Information

Publications322 found, 100 shown here

  1. ncbi Progress in phage display: evolution of the technique and its application
    Tomaz Bratkovic
    Department of Pharmaceutical Biology, Faculty of Pharmacy, University of Ljubljana, Askerceva 7, 1000 Ljubljana, Slovenia
    Cell Mol Life Sci 67:749-67. 2010
    ..Novel display formats, innovative library designs and screening strategies are discussed. I will also briefly review some recent uses of the technology to illustrate its incredible versatility...
  2. pmc Structural and functional bases for broad-spectrum neutralization of avian and human influenza A viruses
    Jianhua Sui
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, Harvard Medical School, 44 Binney Street JFB 826, Boston, Massachusetts 02115, USA
    Nat Struct Mol Biol 16:265-73. 2009
    ..Our data further suggest that this region is recalcitrant to neutralization escape and that nAb-based immunotherapy is a promising strategy for broad-spectrum protection against seasonal and pandemic influenza viruses...
  3. ncbi Yeast surface display for screening combinatorial polypeptide libraries
    E T Boder
    Department of Chemical Engineering, University of Illinois, Urbana 61801, USA
    Nat Biotechnol 15:553-7. 1997
    ..Quantitative flow cytometric analysis enables fine discrimination of kinetic parameters for protein binding to soluble ligands...
  4. ncbi Autoantibody signatures in prostate cancer
    Xiaoju Wang
    Department of Pathology, University of Michigan Medical School, Ann Arbor, MI 48109, USA
    N Engl J Med 353:1224-35. 2005
    ..New biomarkers, such as autoantibody signatures, may improve the early detection of prostate cancer...
  5. ncbi The SPOT-synthesis technique. Synthetic peptide arrays on membrane supports--principles and applications
    Ronald Frank
    AG Molecular Recognition, German Research Centre for Biotechnology GBF, Mascheroder Weg 1, D 38124 Brunswick, Germany
    J Immunol Methods 267:13-26. 2002
    ..Therefore, peptide arrays prepared by the SPOT-technique became quite popular tools for studying numerous aspects of molecular recognition, particularly in the field of molecular immunology...
  6. pmc A compact phage display human scFv library for selection of antibodies to a wide variety of antigens
    Potjamas Pansri
    School of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand
    BMC Biotechnol 9:6. 2009
    ..However, since this technology emerged more than 20 years ago, there have only been a handful reports on the construction and application of phage display antibody libraries world-wide...
  7. ncbi DARPins and other repeat protein scaffolds: advances in engineering and applications
    Ykelien L Boersma
    Department of Biochemistry, University of Zurich, Winterthurerstrasse 190, 8057 Zurich, Switzerland
    Curr Opin Biotechnol 22:849-57. 2011
    ..In this review, progress on DARPins and other repeat protein scaffolds will be discussed. Advances in their design as well as novel applications will be highlighted...
  8. ncbi Rotamer libraries in the 21st century
    Roland L Dunbrack
    Institute for Cancer Research, Fox Chase Cancer Center, 7701 Burholme Avenue, Philadelphia PA 19111, USA
    Curr Opin Struct Biol 12:431-40. 2002
    ....
  9. pmc Bayesian modeling of the yeast SH3 domain interactome predicts spatiotemporal dynamics of endocytosis proteins
    Raffi Tonikian
    Terrence Donnelly Center for Cellular and Biomolecular Research, Banting and Best Department of Medical Research, University of Toronto, Toronto, Ontario, Canada
    PLoS Biol 7:e1000218. 2009
    ....
  10. ncbi Biophysical properties of human antibody variable domains
    Stefan Ewert
    Biochemisches Institut, Universitat Zurich, Winterthurerstr 190, CH 8057 Zurich, Switzerland
    J Mol Biol 325:531-53. 2003
    ..On the basis of these analyses and model structures, we suggest possibilities for further improvement of the biophysical properties of individual frameworks and give recommendations for library design...
  11. ncbi Diversity of phage-displayed libraries of peptides during panning and amplification
    Ratmir Derda
    Department of Chemistry, University of Alberta, Edmonton, AB T6G2G2, Canada
    Molecules 16:1776-803. 2011
    ..This review also highlights techniques that allow elimination of amplification-induced losses of diversity, and how these techniques can be used to improve phage-display selection and enable the identification of novel ligands...
  12. pmc Detection of colonic dysplasia in vivo using a targeted heptapeptide and confocal microendoscopy
    Pei Lin Hsiung
    Department of Pediatrics, Radiology and Microbiology and Immunology, Stanford University School of Medicine, 318 Campus Dr, Rm E 150, Stanford, California 94305, USA
    Nat Med 14:454-8. 2008
    ..This methodology represents a promising diagnostic imaging approach for the early detection of colorectal cancer and potentially of other epithelial malignancies...
  13. ncbi SRP and Sec pathway leader peptides for antibody phage display and antibody fragment production in E. coli
    Holger Thie
    Technische Universitat Braunschweig, Institut für Biochemie und Biotechnologie, Abteilung Biotechnologie, Spielmannstr 7, 38106 Braunschweig, Germany
    N Biotechnol 25:49-54. 2008
    ..The applicability of the SRP pathway to antibody phage display and the production of functional scFvs is shown here for the first time...
  14. ncbi A helper phage to improve single-chain antibody presentation in phage display
    S Rondot
    Molekulargenetisches Labor Prof. Raue, Friedrich-Ebert-Anlage 28, 69117 Heidelberg, Germany
    Nat Biotechnol 19:75-8. 2001
    ..Thus, hyperphage is particularly useful in stoichiometric situations, when there is little chance that a single phage will locate the desired antigen...
  15. ncbi Phage display: concept, innovations, applications and future
    Jyoti Pande
    Department of Medicine, HSC 4N41 McMaster Univ, Hamilton, ON, Canada
    Biotechnol Adv 28:849-58. 2010
    ..Phage display can also be used in conjunction with other methods. The past innovations and those to come promise a bright future for this field...
  16. pmc Crystal structure of full-length KcsA in its closed conformation
    Serdar Uysal
    Department of Biochemistry and Molecular Biology, and Institute for Biophysical Dynamics, University of Chicago, Chicago, IL 60637, USA
    Proc Natl Acad Sci U S A 106:6644-9. 2009
    ..Functional analysis of the full-length KcsA-Fab complex suggests that the C-terminal bundle remains whole during gating. We suggest that this structure likely represents the physiologically relevant closed conformation of KcsA...
  17. ncbi Phage display screening without repetitious selection rounds
    Peter A C 't Hoen
    Center for Human and Clinical Genetics and Leiden Genome Technology Center, Leiden University Medical Center, 2300 RC Leiden, The Netherlands
    Anal Biochem 421:622-31. 2012
    ..We conclude that next generation sequencing can significantly empower phage display screenings by accelerating the finding of specific binders and restraining the number of false positive hits...
  18. ncbi Development of zinc finger domains for recognition of the 5'-ANN-3' family of DNA sequences and their use in the construction of artificial transcription factors
    B Dreier
    Skaggs Institute for Chemical Biology and the Department of Molecular Biology, The Scripps Research Institute, La Jolla, California 92037
    J Biol Chem 276:29466-78. 2001
    ....
  19. ncbi Fully synthetic human combinatorial antibody libraries (HuCAL) based on modular consensus frameworks and CDRs randomized with trinucleotides
    A Knappik
    MorphoSys AG, Lena Christ Str 48, Martinsried Munich, 82152, Germany
    J Mol Biol 296:57-86. 2000
    ..The small number of 49 master genes will allow future improvements to be incorporated quickly, and the separation of the frameworks may help in analyzing why nature has evolved these distinct subfamilies of antibody germline genes...
  20. ncbi Flow-cytometric isolation of human antibodies from a nonimmune Saccharomyces cerevisiae surface display library
    Michael J Feldhaus
    Pacific Northwest National Laboratories, Biological Sciences Division, 902 Battelle Blvd, P O Box 999, Richland, WA 99352, USA
    Nat Biotechnol 21:163-70. 2003
    ..The ability to use multiplex library screening demonstrates the usefulness of this approach for high-throughput antibody isolation for proteomics applications...
  21. pmc Beyond natural antibodies: the power of in vitro display technologies
    Andrew R M Bradbury
    Los Alamos National Laboratory, Los Alamos, New Mexico, USA
    Nat Biotechnol 29:245-54. 2011
    ..The amenability of in vitro display to high-throughput applications broadens the prospects for their wider use in basic and applied research...
  22. ncbi High-throughput generation of synthetic antibodies from highly functional minimalist phage-displayed libraries
    Frederic A Fellouse
    Department of Protein Engineering, Genentech Inc, 1 DNA Way, South San Francisco, CA 94080, USA
    J Mol Biol 373:924-40. 2007
    ....
  23. ncbi Functional proteins from a random-sequence library
    A D Keefe
    Howard Hughes Medical Institute, Department of Molecular Biology, Massachusetts General Hospital, Boston 02114, USA
    Nature 410:715-8. 2001
    ..The frequency of occurrence of functional proteins in random-sequence libraries appears to be similar to that observed for equivalent RNA libraries...
  24. ncbi Reliable cloning of functional antibody variable domains from hybridomas and spleen cell repertoires employing a reengineered phage display system
    A Krebber
    Biochemisches Institut der Universitat Zurich, Switzerland
    J Immunol Methods 201:35-55. 1997
    ..To maximize soluble expression of functional scFvs subsequent to cloning, a compatible vector series to simplify modification, detection, multimerization and rapid purification of recombinant antibody fragments was constructed...
  25. ncbi Domain antibodies: proteins for therapy
    Lucy J Holt
    Domantis Limited, Granta Park, Abington, Cambridge CB1 6GS, UK
    Trends Biotechnol 21:484-90. 2003
    ..dAbs are bioactive as monomers and, owing to their small size and inherent stability, can be formatted into larger molecules to create drugs with prolonged serum half-lives or other pharmacological activities...
  26. ncbi Aggregation-resistant domain antibodies selected on phage by heat denaturation
    Laurent Jespers
    Nat Biotechnol 22:1161-5. 2004
    ..The method should be useful for making aggregation-resistant proteins and for helping to identify features that promote or prevent protein aggregation, including those responsible for misfolding diseases...
  27. pmc Substrates of the Arabidopsis thaliana protein isoaspartyl methyltransferase 1 identified using phage display and biopanning
    Tingsu Chen
    Department of Horticulture, University of Kentucky, Lexington, Kentucky 40546 0312, USA
    J Biol Chem 285:37281-92. 2010
    ..Impaired PIMT activity would hinder protein function in these targets, possibly resulting in poor seed performance...
  28. ncbi Isolation of antigen-binding camelid heavy chain antibody fragments (nanobodies) from an immune library displayed on the surface of Pichia pastoris
    Stefan Ryckaert
    Unit for Molecular Glycobiology, Department for Molecular Biomedical Research, VIB, B 9052 Ghent, Belgium
    J Biotechnol 145:93-8. 2010
    ..This technology for displaying protein libraries on P. pastoris enables the isolation and engineering of antibody-derived molecules in a robust eukaryotic expression host...
  29. ncbi Discovery of novel Streptococcus pneumoniae antigens by screening a whole-genome lambda-display library
    Elisa Beghetto
    Kenton Srl, Pomezia, Rome, Italy
    FEMS Microbiol Lett 262:14-21. 2006
    ..In this work, Spr0075 is first identified as an expressed S. pneumoniae gene product, having an antigenic function during infection...
  30. pmc Facile generation of heat-stable antiviral and antitoxin single domain antibodies from a semisynthetic llama library
    Ellen R Goldman
    Center for Bio Molecular Science and Engineering, US Naval Research Laboratory, Washington, DC 20375, USA
    Anal Chem 78:8245-55. 2006
    ..The ability to rapidly select such rugged antibodies will enhance the reliability of immunoassays by extending shelf life and the capacity to function in hostile environments...
  31. pmc Heterosubtypic neutralizing monoclonal antibodies cross-protective against H5N1 and H1N1 recovered from human IgM+ memory B cells
    Mark Throsby
    Crucell Holland BV, Leiden, The Netherlands
    PLoS ONE 3:e3942. 2008
    ..Furthermore, there are 16 different HA subtypes and no certainty the next pandemic will be caused by an H5 subtype, thus it is important to develop prophylactic and therapeutic interventions that provide heterosubtypic protection...
  32. pmc High-resolution mapping of protein sequence-function relationships
    Douglas M Fowler
    Department of Genome Sciences, University of Washington, Seattle, WA, USA
    Nat Methods 7:741-6. 2010
    ..Our approach could be applied to many in vitro or in vivo protein assays, providing a general means for understanding how protein function relates to sequence...
  33. pmc A new peptide ligand for targeting human carbonic anhydrase IX, identified through the phage display technology
    Vasileios Askoxylakis
    Department of Radiooncology and Radiation Therapy, University of Heidelberg, Heidelberg, Germany
    PLoS ONE 5:e15962. 2010
    ..Ligands binding this target may be used to visualize hypoxia, tumor manifestation or treat tumors by endoradiotherapy...
  34. ncbi Quantification of PDZ domain specificity, prediction of ligand affinity and rational design of super-binding peptides
    Urs Wiedemann
    Forschungsinstitut fur Molekulare Pharmakologie, Robert Rossle Str 10, 13125 Berlin, Germany
    J Mol Biol 343:703-18. 2004
    ..specificity as a function of ligand sequence-dependent affinity contributions by statistical analysis of peptide library screens for the AF6, ERBIN and SNA1 (alpha-1-syntrophin) PDZ domains...
  35. ncbi Generating recombinant antibodies to the complete human proteome
    Stefan Dubel
    Technische Universitat Braunschweig, Institute of Biochemistry and Biotechnology, D 38106 Braunschweig, Germany
    Trends Biotechnol 28:333-9. 2010
    ..This opinion article summarizes opportunities for the generation of antibodies for proteome research using in vitro technologies...
  36. ncbi Epitope identification and discovery using phage display libraries: applications in vaccine development and diagnostics
    Lin Fa Wang
    CSIRO Livestock Industries, Australian Animal Health Laboratory, Geelong 3220, Victoria, Australia
    Curr Drug Targets 5:1-15. 2004
    ..Instead, this review aims to focus on specific applications related to the discovery and identification of epitopes which have potential as vaccine candidates or can be used in disease diagnosis...
  37. ncbi Phage versus phagemid libraries for generation of human monoclonal antibodies
    David O'Connell
    Department of Anesthesia and Pharmaceutical Chemistry, University of California, San Francisco, Rm 3C 38, San Francisco General Hospital, 1001 Potrero Ave, 94110, USA
    J Mol Biol 321:49-56. 2002
    ..This could be overcome by utilizing the scFv in a multivalent format, by affinity maturation, or by converting the library to monovalent display after the first round of selection...
  38. pmc Mapping B-cell epitopes of hepatitis C virus E2 glycoprotein using human monoclonal antibodies from phage display libraries
    F Bugli
    Istituti di Microbiologia, , , 00168 Rome, Italy
    J Virol 75:9986-90. 2001
    ....
  39. ncbi Random-peptide libraries and antigen-fragment libraries for epitope mapping and the development of vaccines and diagnostics
    M B Irving
    Department of Molecular Biology and Biochemistry, Simon Fraser University, BC V5A 1S6, Burnaby, Canada
    Curr Opin Chem Biol 5:314-24. 2001
    ..These may prove useful for diagnosis and, possibly, in determining disease etiology...
  40. ncbi Generation of high-affinity human antibodies by combining donor-derived and synthetic complementarity-determining-region diversity
    René Michael Hoet
    Nat Biotechnol 23:344-8. 2005
    ..This very often circumvents the need for affinity maturation, accelerating discovery of antibody drug candidates...
  41. ncbi Isolation of engineered, full-length antibodies from libraries expressed in Escherichia coli
    Yariv Mazor
    Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, Texas 78712, USA
    Nat Biotechnol 25:563-5. 2007
    ..Screening of a library constructed from an immunized animal yielded several antibodies with nanomolar affinities toward the protective antigen of Bacillus anthracis...
  42. ncbi The functional capacity of the natural amino acids for molecular recognition
    Sara Birtalan
    Department of Protein Engineering, Genentech Inc, 1 DNA Way, South San Francisco, CA 94080, USA
    Mol Biosyst 6:1186-94. 2010
    ..Moreover, our findings illuminate the fundamental principles underlying protein-protein interactions and provide valuable guidelines for engineering synthetic binding proteins with functions beyond the scope of natural proteins...
  43. ncbi Efficient cancer therapy with a nanobody-based conjugate
    Virna Cortez-Retamozo
    Department of Molecular and Cellular Interactions, Flanders Interuniversity Institute for Biotechnology, Vrije Universiteit Brussel, Brussels, Belgium
    Cancer Res 64:2853-7. 2004
    ..The easy generation and manufacturing yield of nanobody-based conjugates together with their potent antitumor activity make nanobodies promising vehicles for new generation cancer therapeutics...
  44. ncbi High affinity, developability and functional size: the holy grail of combinatorial antibody library generation
    Dirk Ponsel
    MorphoSys AG, Lena Christ Strasse 48, 82152 Martinsried, Germany
    Molecules 16:3675-700. 2011
    ..More recently a major focus on reduction of unwanted side effects through reduced immunogenicity and improved overall biophysical behavior has led to a re-evaluation of library design...
  45. pmc On the influence of vector design on antibody phage display
    Glenn Soltes
    Cangene Corporation, 3403 American Drive, Mississauga, Ontario L4V 1T4, Canada
    J Biotechnol 127:626-37. 2007
    ..However, phage production was positively correlated to phage infectivity. In summary, this study demonstrates simultaneously optimization of multiple and independent features of importance for phage selection...
  46. pmc Probing a protein-protein interaction by in vitro evolution
    George Thom
    Cambridge Antibody Technology, Milstein Building, Granta Park, Cambridge CB1 6GH, United Kingdom
    Proc Natl Acad Sci U S A 103:7619-24. 2006
    ....
  47. ncbi Selection of full-length IgGs by tandem display on filamentous phage particles and Escherichia coli fluorescence-activated cell sorting screening
    Yariv Mazor
    Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, TX 78712, USA
    FEBS J 277:2291-303. 2010
    ....
  48. ncbi Filamentous phage display in the new millennium
    John W Kehoe
    Biosciences Division, Argonne National Laboratory, Building 202, Argonne, Illinois 60439, USA
    Chem Rev 105:4056-72. 2005
  49. ncbi Signal sequences directing cotranslational translocation expand the range of proteins amenable to phage display
    Daniel Steiner
    Department of Biochemistry, University of Zurich, Winterthurerstrasse 190, 8057 Zurich, Switzerland
    Nat Biotechnol 24:823-31. 2006
    ..We named this method 'SRP phage display' and envision broad applicability, especially when displaying cDNA libraries or very stable and fast-folding proteins from libraries of alternative scaffolds...
  50. pmc Identification of a conserved B-cell epitope on reticuloendotheliosis virus envelope protein by screening a phage-displayed random peptide library
    Mei Xue
    Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, Harbin, P R China
    PLoS ONE 7:e49842. 2012
    ..B-cell epitopes on the gp90 protein of REV have not been well studied and reported...
  51. ncbi Affilin-novel binding molecules based on human gamma-B-crystallin, an all beta-sheet protein
    Hilmar Ebersbach
    Scil Proteins GmbH, Heinrich Damerow Str 1, 06120 Halle Saale, Germany
    J Mol Biol 372:172-85. 2007
    ..We conclude that the intrinsically stable and easily expressed gamma-B-crystallin provides a suitable framework for the generation of novel binding molecules...
  52. ncbi Peptides panned from a phage-displayed random peptide library are useful for the detection of Bacillus anthracis surrogates B. cereus 4342 and B. anthracis Sterne
    Shilpakala Sainath Rao
    Section of Cell Biology, Laboratory of Cellular Hematology, Center for Biologics Evaluation and Research, FDA, Bethesda, MD 20892, USA
    Biochem Biophys Res Commun 395:93-8. 2010
    ..In this study, we screened a commercially available bacteriophage-displayed random peptide library using Bacillus cereus 4342 cells as bait to identify peptides that could be used for detection of Bacillus...
  53. ncbi The human combinatorial antibody library HuCAL GOLD combines diversification of all six CDRs according to the natural immune system with a novel display method for efficient selection of high-affinity antibodies
    Christine Rothe
    MorphoSys AG, Lena Christ Strasse 48, 82152 Martinsried, Germany
    J Mol Biol 376:1182-200. 2008
    ..Furthermore, the data presented in this article demonstrate that CysDisplay is a robust and broadly applicable display technology even for high-throughput applications...
  54. ncbi Use of baculovirus MHC/peptide display libraries to characterize T-cell receptor ligands
    Frances Crawford
    Integrated Department of Immunology, National Jewish Medical and Research Center, Denver, CO 80206, USA
    Immunol Rev 210:156-70. 2006
    ..We have used these libraries to examine the role of MHCII-bound peptides in the presentation of the staphylococcal enterotoxin A (SEA) and to manipulate an MHCI tumor-associated antigen...
  55. pmc Landscape phage ligands for PC3 prostate carcinoma cells
    P K Jayanna
    Department of Pathobiology, College of Veterinary Medicine, Auburn University, Auburn, AL 36849, USA
    Protein Eng Des Sel 23:423-30. 2010
    ..The phage ligands and their fusion coat proteins can be used as navigating modules in both therapeutic and diagnostic approaches to prostate carcinoma...
  56. pmc Antiangiogenic targeting liposomes increase therapeutic efficacy for solid tumors
    De Kuan Chang
    Institute of Cellular and Organismic Biology, Academia Sinica, Taipei, Taiwan
    J Biol Chem 284:12905-16. 2009
    ..Our results indicate that the tumor-homing peptides can be used specifically target tumor vasculature and have the potential to improve the systemic treatment of patients with solid tumors...
  57. pmc Display technologies: application for the discovery of drug and gene delivery agents
    Anna Sergeeva
    Department of Blood and Marrow Transplantation, The University of Texas M D Anderson Cancer Center, Houston, Texas 77030, USA
    Adv Drug Deliv Rev 58:1622-54. 2006
    ..Prospective applications of targeted compounds derived from display libraries in the discovery of targeted drugs and gene therapy vectors are discussed...
  58. pmc Corruption of phage display libraries by target-unrelated clones: diagnosis and countermeasures
    William D Thomas
    Division of Biological Sciences, University of Missouri, Columbia, MO 65211, USA
    Anal Biochem 407:237-40. 2010
    ..The founder's infectivity advantage spread by simple recombination to clones displaying different peptides. We propose measures for minimizing TUP corruption...
  59. pmc Rapid determination of multiple linear kinase substrate motifs by mass spectrometry
    Arminja N Kettenbach
    Norris Cotton Cancer Center, Lebanon, NH 03756, USA
    Chem Biol 19:608-18. 2012
    ..Furthermore, we identify specific and distinguishable motif elements for the four members of the polo-like kinase (Plk) family and verify members of these motif elements for Plk1 in vivo...
  60. ncbi Combining phage and staphylococcal surface display for generation of ErbB3-specific Affibody molecules
    Nina Kronqvist
    Department of Molecular Biotechnology, School of Biotechnology, Royal Institute of Technology KTH, AlbaNova University Center, SE 106 91 Stockholm
    Protein Eng Des Sel 24:385-96. 2011
    ..Potential future applications, such as radionuclide-based diagnosis and treatment of human cancers are discussed...
  61. ncbi Design, production and molecular structure of a new family of artificial alpha-helicoidal repeat proteins (αRep) based on thermostable HEAT-like repeats
    Agathe Urvoas
    Institut de Biochimie Moléculaire et Cellulaire IBBMC, Univ Paris Sud, UMR 8619, Orsay, F 91405 Orsay, France
    J Mol Biol 404:307-27. 2010
    ..This fortuitous binding site could later prove to be a useful binding site for other low molecular mass partners...
  62. ncbi Preferential binding of peptides to graphene edges and planes
    Sang N Kim
    Materials and Manufacturing Directorate, Air Force Research Laboratory, Wright Patterson Air Force Base, Ohio 45433, USA
    J Am Chem Soc 133:14480-3. 2011
    ..Here we show that peptides identified from a combinatorial phage display peptide library assemble preferentially to the edge or planar surface of graphene and can affect the electronic properties of ..
  63. pmc Development of anti-infectives using phage display: biological agents against bacteria, viruses, and parasites
    Johnny X Huang
    Institute for Molecular Bioscience, The University of Queensland, St Lucia, Brisbane, Australia
    Antimicrob Agents Chemother 56:4569-82. 2012
    ..Advances in screening, manufacturing, and humanization technologies now mean that phage display can make a significant contribution in the fight against clinically important pathogens...
  64. pmc Isoform-specific monobody inhibitors of small ubiquitin-related modifiers engineered using structure-guided library design
    Ryan N Gilbreth
    Department of Biochemistry and Molecular Biology, University of Chicago, 929 East 57th Street, Chicago, IL 60637, USA
    Proc Natl Acad Sci U S A 108:7751-6. 2011
    ..The monobodies inhibited SUMO1/SIM interactions and, unexpectedly, also inhibited SUMO1 conjugation. These high-affinity and isoform-specific inhibitors will enhance mechanistic and cellular investigations of SUMO biology...
  65. pmc Protective activity of Streptococcus pneumoniae Spr1875 protein fragments identified using a phage displayed genomic library
    Angela Cardaci
    The Elie Metchnikoff Department, Universita di Messina, Messina, Italy
    PLoS ONE 7:e36588. 2012
    ..As the R4 fragment conferred immunoprotection from experimental sepsis, selected antigenic fragments of Spr1875 may be useful for the development of a pneumococcal protein-based vaccine...
  66. pmc Development of a novel mammalian cell surface antibody display platform
    Chen Zhou
    Protein Science, Amgen Inc, Thousand Oaks, CA, USA
    MAbs 2:508-18. 2010
    ..Antibodies with significantly improved neutralizing activity have been identified from a randomly mutagenized library, demonstrating the power of this platform in screening and selecting antibody therapeutics...
  67. ncbi Engineered high-affinity affibody molecules targeting platelet-derived growth factor receptor β in vivo
    M Lindborg
    Affibody AB, Stockholm, Sweden
    J Mol Biol 407:298-315. 2011
    ..The PDGFRβ-specific Affibody molecule also accumulated around tumoral blood vessels in a model of spontaneous insulinoma, confirming a potential for in vivo targeting...
  68. pmc Virus-polymer hybrid nanowires tailored to detect prostate-specific membrane antigen
    Jessica A Arter
    Department of Chemistry, University of California, Irvine, California 92697 2025, United States
    Anal Chem 84:2776-83. 2012
    ....
  69. pmc A human PrM antibody that recognizes a novel cryptic epitope on dengue E glycoprotein
    Annie Hoi Yi Chan
    Bio Defence Programme, DMERI, DSO National Laboratories, Singapore, Singapore
    PLoS ONE 7:e33451. 2012
    ..These findings suggest that generation of anti-prM antibodies that enhance DENV infection may not be completely avoided even with immunization strategies employing E protein alone or subunits of E proteins...
  70. pmc Structural insights for engineering binding proteins based on non-antibody scaffolds
    Ryan N Gilbreth
    Department of Biochemistry and Molecular Biology, The University of Chicago, Chicago, IL 60637, USA
    Curr Opin Struct Biol 22:413-20. 2012
    ..Furthermore, the structural features of these synthetic proteins produced under tightly controlled, directed evolution deepen our understanding of the underlying principles governing molecular recognition...
  71. pmc Protease-resistant peptide ligands from a knottin scaffold library
    Jennifer A Getz
    Department of Chemical Engineering, University of California, Santa Barbara, California 93106, United States
    ACS Chem Biol 6:837-44. 2011
    ..More generally, this strategy may prove useful in the development of stable peptide ligands suitable for in vivo applications...
  72. pmc Purification of phage display-modified bacteriophage T4 by affinity chromatography
    Anna Oślizło
    Bacteriophage Laboratory, Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, R Weigla 12, Wroclaw, Poland
    BMC Biotechnol 11:59. 2011
    ..The phage was propagated in bacteria expressing fusions of the phage protein Hoc with affinity tags from bacterial plasmids, independently from the phage expression system...
  73. pmc Global identification of peptidase specificity by multiplex substrate profiling
    Anthony J O'Donoghue
    Department of Pharmaceutical Chemistry, University of California, San Francisco, USA
    Nat Methods 9:1095-100. 2012
    ..Additionally, the proteolytic secretions from schistosome parasitic flatworm larvae and a pancreatic cancer cell line were deconvoluted in a subtractive strategy using class-specific peptidase inhibitors...
  74. ncbi Isolation of ZnO-binding 12-mer peptides and determination of their binding epitopes by NMR spectroscopy
    Dirk Rothenstein
    Institute for Materials Science, University of Stuttgart, 70569 Stuttgart, Germany
    J Am Chem Soc 134:12547-56. 2012
    ..oxygen-terminated sides of single-crystalline ZnO (0001) and (000-1) substrates, were selected from a random peptide library using the phage display technique...
  75. ncbi Anticalins small engineered binding proteins based on the lipocalin scaffold
    Michaela Gebauer
    Lehrstuhl fur Biologische Chemie, Technische Universitat Munchen, Freising Weihenstephan, Germany
    Methods Enzymol 503:157-88. 2012
    ....
  76. pmc Design of a binding scaffold based on variable lymphocyte receptors of jawless vertebrates by module engineering
    Sang Chul Lee
    Department of Biological Sciences, Korea Advanced Institute of Science and Technology KAIST, Daejeon, 305 701, Korea
    Proc Natl Acad Sci U S A 109:3299-304. 2012
    ..We demonstrate general applicability of the scaffold by selecting repebodies with different binding affinities for interleukin-6 using phage display...
  77. ncbi Improved breadth and potency of an HIV-1-neutralizing human single-chain antibody by random mutagenesis and sequential antigen panning
    Mei Yun Zhang
    Human Immunovirology and Computational Biology Group, LECB, CCR, National Cancer Institute Frederick, NIH, Frederick, MD 21702, USA
    J Mol Biol 335:209-19. 2004
    ....
  78. pmc C-end rule peptides mediate neuropilin-1-dependent cell, vascular, and tissue penetration
    Tambet Teesalu
    Vascular Mapping Center, The Burnham Institute for Medical Research at University of California, Santa Barbara, CA 93106 9610, USA
    Proc Natl Acad Sci U S A 106:16157-62. 2009
    ..The peptides were able to take payloads up to the nanoparticle size scale deep into extravascular tissue. Our observations have implications in drug delivery and penetration of tissue barriers and tumors...
  79. pmc Non-human primate immune libraries combined with germline humanization: an (almost) new, and powerful approach for the isolation of therapeutic antibodies
    Thibaut Pelat
    Groupe de Biotechnologie des Anticorps, Département de Biologie des Agents Transmissibles, Centre de Recherche du Service de Sante des Armees, La Tronche, France
    MAbs 1:377-81. 2009
    ..In this report, the various approaches will be compared, and advantages of the two-step NHP-based method, as well as corresponding intellectual property aspects, will be discussed...
  80. ncbi Challenges in optimizing a prostate carcinoma binding peptide, identified through the phage display technology
    Vasileios Askoxylakis
    Department of Radiooncology and Radiation Therapy, University of Heidelberg, INF 400, 69120 Heidelberg, Germany
    Molecules 16:1559-78. 2011
    ..Our results demonstrate challenges in optimizing peptides, identified through phage display libraries, and show that careful investigation of modified derivatives is necessary in order to improve their characteristics...
  81. pmc High-affinity TCRs generated by phage display provide CD4+ T cells with the ability to recognize and kill tumor cell lines
    Yangbing Zhao
    Surgery Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    J Immunol 179:5845-54. 2007
    ..Although the biological activity of these two relatively low-affinity TCRs was comparable to wild-type reactivity in CD8(+) T cells, introduction of these TCR dramatically increased the reactivity of CD4(+) T cells to tumor cell lines...
  82. ncbi Inhibition of Wnt signaling by Dishevelled PDZ peptides
    Yingnan Zhang
    Department of Protein Engineering, Genentech, Inc, South San Francisco, California, USA
    Nat Chem Biol 5:217-9. 2009
    ..These peptide ligands inhibit Wnt/beta-catenin signaling in cells, showing that Dishevelled PDZ domains are potential targets for small-molecule cancer therapeutics...
  83. ncbi Design and evaluation of a 6-mer amyloid-beta protein derived phage display library for molecular targeting of amyloid plaques in Alzheimer's disease: Comparison with two cyclic heptapeptides derived from a randomized phage display library
    Lionel Larbanoix
    Department of General, Organic and Biomedical Chemistry, NMR and Molecular Imaging Laboratory, University of Mons, Avenue Maistriau 19, B 7000 Mons, Belgium
    Peptides 32:1232-43. 2011
    ..To conclude, these peptides have high potential for molecular targeting of amyloid plaques, either as carriers of molecular imaging and therapeutic compounds or as amyloid fiber disrupting agents...
  84. ncbi Comparison of the efficiency of antibody selection from semi-synthetic scFv and non-immune Fab phage display libraries against protein targets for rapid development of diagnostic immunoassays
    Conrad E Z Chan
    Biodefence Therapeutics Laboratory, Defence Medical and Environmental Research Institute, DSO National Laboratories, 27 Medical Drive, 13 00, S 117510, Singapore
    J Immunol Methods 373:79-88. 2011
    ..However, the ease and speed with which full-length IgG could be generated from the human-derived Fab library makes screening this type of library the preferable method for rapid antibody generation for diagnostic assay development...
  85. ncbi Epitope prediction based on random peptide library screening: benchmark dataset and prediction tools evaluation
    Pingping Sun
    Faculty of Chemistry, Northeast Normal University, Changchun 130024, China
    Molecules 16:4971-93. 2011
    Epitope prediction based on random peptide library screening has become a focus as a promising method in immunoinformatics research...
  86. ncbi High-density functional display of proteins on bacteriophage lambda
    Amita Gupta
    Department of Biochemistry, University of Delhi South Campus, New Delhi 110021, India
    J Mol Biol 334:241-54. 2003
    ..The high-density display of foreign proteins on bacteriophage lambda should be extremely useful in studying low-affinity protein-protein interactions more efficiently compared to the M13 phage-based system...
  87. ncbi Identification of peptide inhibitors of transforming growth factor beta 1 using a phage-displayed peptide library
    Javier Dotor
    Division of Hepatology and Gene Therapy, Center for Applied Medical Research CIMA, University of Navarra, Avda Pio XII, 55 31008 Pamplona, Spain
    Cytokine 39:106-15. 2007
    ..this aim we searched for peptides with binding affinity for TGFbeta1 using a phage-displayed random 15-mer peptide library. Some peptides thus identified blocked activity of TGFbeta1 in vitro, as measured by their capacity to ..
  88. ncbi Reduction of dynamic protein concentration range of biological extracts for the discovery of low-abundance proteins by means of hexapeptide ligand library
    Luc Guerrier
    Bio Rad Laboratories c o CEA Saclay, DSV iBiTecS, 91191 Gif sur Yvette, France
    Nat Protoc 3:883-90. 2008
    ..Unbound components are washed out and captured species are finally desorbed. The entire sample treatment process takes about half a day and yields a protein solution that could be used as such for mass spectrometry investigations...
  89. ncbi Generation of tumour-necrosis-factor-alpha-specific affibody molecules capable of blocking receptor binding in vitro
    Andreas Jonsson
    Division of Molecular Biotechnology, Royal Institute of Technology KTH, AlbaNova University Center, SE 106 91 Stockholm, Sweden
    Biotechnol Appl Biochem 54:93-103. 2009
    ..The potential implication of the results for the future design of affibody-based reagents for the diagnosis of inflammation is discussed...
  90. ncbi Tuning ligand affinity, specificity, and folding stability of an engineered lipocalin variant -- a so-called 'anticalin' -- using a molecular random approach
    Steffen Schlehuber
    Lehrstuhl fur Biologische Chemie, Technische Universitat Munchen, Freising Weihenstephan, Germany
    Biophys Chem 96:213-28. 2002
    ..Our present findings support the notion that anticalins provide a useful class of compact and robust ligand-receptor proteins that can be tailored for practical demands...
  91. ncbi Antibody fragments selected by phage display against the nuclear localization signal of the HIV-1 Vpr protein inhibit nuclear import in permeabilized and intact cultured cells
    A Krichevsky
    Department of Biological Chemistry, The Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem, Israel
    Virology 305:77-92. 2003
    ..The use of the described inhibitory anti-VprN scFv fragments to study nuclear import of viral karyophilic proteins and their therapeutic potential is discussed...
  92. ncbi Novel human monoclonal antibodies to insulin-like growth factor (IGF)-II that potently inhibit the IGF receptor type I signal transduction function
    Yang Feng
    Protein Interactions Group, Nanobiology Program, Center for Cancer Research, National Cancer Institute Frederick, NIH Building 469, Frederick, MD 21702 1201, USA
    Mol Cancer Ther 5:114-20. 2006
    ....
  93. ncbi A broad-spectrum fluorescence-based peptide library for the rapid identification of protease substrates
    Daniel A Thomas
    Proteolysis Research Group, School of Biochemistry and Microbiology, Leeds Institute of Genetics, Health and Therapeutics, University of Leeds, Leeds, UK
    Proteomics 6:2112-20. 2006
    ..The thorough validation of this 'small but smart' peptide library with representatives from each of the four mechanistic protease classes indicates that the REPLi will be ..
  94. pmc Immunogenic display of diverse peptides, including a broadly cross-type neutralizing human papillomavirus L2 epitope, on virus-like particles of the RNA bacteriophage PP7
    Jerri do Carmo Caldeira
    Department of Molecular Genetics and Microbiology, University of New Mexico School of Medicine, Albuquerque, NM 87131, USA
    Vaccine 28:4384-93. 2010
    ..Thus, PP7 VLPs are well-suited for the display of a wide diversity of peptides in a highly immunogenic format...
  95. ncbi MimoDB: a new repository for mimotope data derived from phage display technology
    Beibei Ru
    Key Laboratory for NeuroInformation of Ministry of Education, School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, Sichuan, China
    Molecules 15:8279-88. 2010
    ..For experimental biologists, their results can be searched against the MimoDB database to exclude possible target-unrelated peptides. The MimoDB database is freely accessible at http://immunet.cn/mimodb/...
  96. ncbi Development of a troponin I biosensor using a peptide obtained through phage display
    Jun Wu
    Department of Chemical Engineering, Columbia University, New York, New York, USA
    Anal Chem 82:8235-43. 2010
    ....
  97. ncbi Coevolution of PDZ domain-ligand interactions analyzed by high-throughput phage display and deep sequencing
    Andreas Ernst
    Banting and Best Department of Medical Research, and the Terrence Donnelly Center for Cellular and Biomolecular Research, University of Toronto, 160 College Street, Toronto, Ontario, Canada
    Mol Biosyst 6:1782-90. 2010
    ..The details of intramolecular cooperativity remain to be elucidated, but nonetheless, we have established a general methodology that can be used to explore protein evolution in a systematic yet rapid manner...
  98. ncbi T7 phage display of Ep15 peptide for the detection of WNV IgG
    Sebastien Herrmann
    Department of Biotechnology Engineering, Ben Gurion University of the Negev, Beer Sheva 84105, Israel
    J Virol Methods 141:133-40. 2007
    ....
  99. ncbi Improved microtitre plate production of single chain Fv fragments in Escherichia coli
    Michael Hust
    Technische Universitat Braunschweig, Institut für Biochemie und Biotechnologie, Abteilung Biotechnologie, Spielmannstr 7, 38106 Braunschweig, Germany
    N Biotechnol 25:424-8. 2009
    ..Although it was not possible to deduce a single set of optimal parameters applicable to all the tested scFvs, a combined protocol was developed which improved the expression of scFv fragments over standard methods...
  100. ncbi Phage-display and correlated mutations identify an essential region of subdomain 1C involved in homodimerization of Escherichia coli FtsA
    Daniele Carettoni
    GlaxoSmithKline Medicines Research Center, Via Fleming 4, 37135 Verona, Italy
    Proteins 50:192-206. 2003
    ..This mutant failed to complement conditional lethal alleles of ftsA, demonstrating that amino acids 126-133 play an essential role in E. coli...
  101. ncbi N-Methylated peptide inhibitors of beta-amyloid aggregation and toxicity. Optimization of the inhibitor structure
    Nicoleta Kokkoni
    Manchester Interdisciplinary Biocentre, The University of Manchester, 131 Princess Street, Manchester, M1 7DN, United Kingdom
    Biochemistry 45:9906-18. 2006
    ..The most active compound, d-[(chGly)-(Tyr)-(chGly)-(chGly)-(mLeu)]-NH(2), was more active than all previously reported peptide inhibitors. Its related non-N-methylated analogues were insoluble and toxic...

Research Grants62

  1. IMPROVED ASSAYS FOR SPECIFICALLY MEASURING COLLAGENASE-3
    MICHAEL VITEK; Fiscal Year: 2004
    ..Phage display results are reported for stromelysin 1, gelatinase A and gelatinase B while a synthetic peptide library was used to determine the substrate specificity of collagenase 1...
  2. Novel Peptide Libraries for Early Cancer Detection
    Krzysztof Darlak; Fiscal Year: 2007
    ..This requires the selective concentration of classes of proteins, e.g. PTKs using Targeted Combinatorial Peptide Library (TCPL) technology...
  3. Enhanced iron removal with peptide linked chelators targeted to ferritin
    Elizabeth C Theil; Fiscal Year: 2010
    ..was recently identified in a group of five binding peptides isolated from a combinatorial (phage display) peptide library of 109, which increased chelation of iron from ferritin in solution...
  4. Tissue-specific delivery of probes by control of membrane trafficking of endoprot
    Blake Peterson; Fiscal Year: 2010
    ..moiety will play two roles: it will enhance the Vd of the members of the peptide library, improving access to all tissues in vivo, and it will selectively display peptide substrates to the subset of ..
  5. Brown Adipose Tissue Vasculature Probes for Non-Invasive Imaging
    Mikhail G Kolonin; Fiscal Year: 2011
    ..BAT-homing ligands will be isolated by integrating a screen of a phage-displayed combinatorial peptide library in vivo with the bioinformatics platform established by our group...
  6. Fibronectin-binding Protein of Treponema Denticola in Periodontal Disease
    Xiaoping Xu; Fiscal Year: 2012
    ..In Aim 2, we will screen a random peptide library to identify the binding site of Fbp for FN and subsequently verify the binding site by site-specific ..
  7. Therapeutic correction of deltaF508-CFTR cystic fibrosis by a novel PDZ12 drug.
    Alvin Bach; Fiscal Year: 2013
    ..Synthesis of an optimized clinical lead AT010-derived peptide library: AT010 analogs designed to have stability, efficacy, low cost of manufacture, simple predicted CMC, non-..
  8. Molecular analysis of CHD4
    Tatiana G Kutateladze; Fiscal Year: 2013
    ..The histone specificities and binding affinities will be examined by western blot assays, peptide library screening, NMR, and fluorescence spectroscopy...
  9. Dissecting ubiquitin pathway selectivity with Ub-isopeptide microarrays
    James Strickler; Fiscal Year: 2010
    ..In this application, we propose to construct a peptide library linked to Ub through a physiologically relevant isopeptide bond between the carboxyl of the C-terminal Gly of ..
  10. Tranthyretin's Regulatory Role in Beta-Amyloid Aggregation and Toxicity
    Regina M Murphy; Fiscal Year: 2013
    ..Further definition of the binding site will be obtained by screening for A? binding to peptide library derived from overlapping sequences of TTR, and by targeted alanine mutagenesis...
  11. Enhancing Intestinal &Brain Uptake of Anti-AIDS Drugs
    Patrick J Sinko; Fiscal Year: 2013
    ....
  12. PKD Isoforms in Heart
    Susan F Steinberg; Fiscal Year: 2013
    ..Aim #1 will use state-of-the- art peptide library screens to define PKD isoform substrate specificity...
  13. Anaerobic shock as a novel treatment for tuberculosis
    Xilin Zhao; Fiscal Year: 2010
    ..An in vivo cyclic peptide library will also be constructed and screened for autolysis- inducing activities similar to that conferred by ..
  14. Synthesis and Screening of Peptide and Protein Based Rhenium Carbonyl Compounds a
    Christopher J Ziegler; Fiscal Year: 2012
    ..on histidine and including cysteine and methionine as well as pyridine imine coupled amino acids, 2) Screen a peptide library for strong ReI(CO)3 binders to assist with the design of his-tag sequences, 3) modify proteins with ReI(CO)3 ..
  15. Design of novel complement inhibitors
    John D Lambris; Fiscal Year: 2010
    ..In Aim 2, phage peptide libraries and in silico screening of public and commercial compound libraries will be used to identify complement inhibitors targeting factor B, factor D, C3, C5, and C1. ..
  16. DnaK Protein Inhibitor Antimicrobials
    Michael Sturgess; Fiscal Year: 2003
    ..This line of investigations will include a peptide library with fixed delivery and variable DnaK-binding residues as well as de novo design of Hsp70 inhibitors...
  17. Molecular Targeting of Lymphatic Endothelial Receptors for Ligand-directed Imagin
    RENATA contact PASQUALINI; Fiscal Year: 2010
    ..to different tissues are recovered after intravenous administration of a bacteriophage (phage) random peptide library. Here we propose to adapt this selection process and perform an ex vivo phage screening on lymphatic vessels ..
  18. Radiolabeled RGD Peptides for Breast Cancer Imaging and Therapy
    Zhen Cheng; Fiscal Year: 2010
    ..The purpose of this proposal is to develop a multimeric RGD peptide library, from which we expect to identify highly effective integrin-specific agents for diagnosis and treatment of ..
  19. MOLECULAR CHARACTERIZATION OF FIV
    John H Elder; Fiscal Year: 2013
    ..Sera from Progressor and Non-progressor cats will be analyzed against an overlapping peptide library to map epitopes recognized by infected cats as a function of disease progression]...
  20. Next Generation Oncolytic Adenovirus for Advanced Pancreatic Cancer Treatment
    Masato Yamamoto; Fiscal Year: 2013
    ..One promising way to overcome this obstacle is high-throughput screening of the high-diversity ligand- peptide library in the format of Ad capsid from the beginning...
  21. Acquisition of 700 MHz NMR for Automated Chemical/Peptide Library Screening
    Dale F Mierke; Fiscal Year: 2010
    ....
  22. Protein Modification: Isoaspartic Acid
    Zhaohui Sunny Zhou; Fiscal Year: 2013
    ..Isoaspartyl peptide library screening, enzyme kinetic characterization, proteomic analysis and protein structural modeling will be ..
  23. Bone-seeking and cell-targeting non-viral vectors for BMP-2 gene delivery
    Chuanbin Mao; Fiscal Year: 2011
    ..our central hypotheses: (1) Selection of bone-seeking and cell-targeting peptides from a virus-based random peptide library;(2) Biomimetic assembly of BMP-2-gene-bearing nanoparticles and the bone-seeking and cell-targeting peptides ..
  24. Neutralizing Antibody &AAV FIX Gene Therapy
    Richard J Samulski; Fiscal Year: 2013
    ..Jude FIX trial serum samples, humanized mouse models and peptide library to understand relationship between capsid antigen presentation and Ab response...
  25. Immunoassay for diagnosis of Babesia infection
    ANDREW E LEVIN; Fiscal Year: 2013
    ..of putative Babesia antigenic sequences that will be screened with babesiosis patient serum in an overlapping peptide library format, a strategy that has led to discovery of a number of antigens of diagnostic value...
  26. Optimizing Gag CTL Epitopes to Improve Immunogenicity
    June Kan Mitchell; Fiscal Year: 2010
    ..The agonist p41 peptide of SL9, identified by probing a large combinatorial peptide library with SL9-specific CD8+ T cells, elicits stable SL9-crossreactive T cells...
  27. Characterization of antitumor auto-antibodies using combinatorial peptide librari
    Wlodek Mandecki; Fiscal Year: 2012
    ..to develop an approach to detect auto-antibodies to tumor-associated antigens using an encoded combinatorial peptide library synthesized on PharmaSeq's light-activated radio-frequency p-Chips...
  28. The Structural and Functional Basis of HLA-associated Drug Hypersensitivity
    David A Ostrov; Fiscal Year: 2013
    ..We will perform affinity measurements of a combinatorial peptide library in the presence or absence of the drug to the relevant HLA-B molecule and confirm drug effects detected in ..
  29. PROTEIN KINASE SIGNALING PATHWAYS
    Lewis C Cantley; Fiscal Year: 2012
    ..With support from this grant over the past decade we have developed oriented peptide library approaches that have begun to explain how protein kinases phosphorylate specific substrates, on the basis of ..
  30. Inhibitor-sensitive and -resistant EGFR mutants from lung cancer and glioblastoma
    Matthew L Meyerson; Fiscal Year: 2013
    ..and Aim 3) Analyze the substrate specificity of wild- type and cancer-derived EGFR mutants through peptide library array experiments, mass spectrometry-based phosphoproteomics, and crystal structure determination...
  31. Phage-Mimetic Nanorods for Targeted Breast Cancer Treatment
    Chuanbin Mao; Fiscal Year: 2010
    ..1: Identify tumor-homing peptides that are fused to pVIII of nanorod-like phage from a phage-displayed random peptide library. Aim 2: Establish the biomimetic assembly of tumor-homing phage proteins on the DNA-immobilized AuNRs to form ..
  32. Substrate Profiling of Protein Tyrosine Phosphatases
    Dehua Pei; Fiscal Year: 2013
    ..In this approach, the substrate specificity of PTP is systematically determined by screening a combinatorial peptide library and the consensus motif(s) is used to search protein and genomic databases to identify potential protein ..
  33. The Function of IKKeplison in Breast Cancer
    RHINE SHEN; Fiscal Year: 2011
    ..An unbiased approach will also be taken with high- throughput screening of a positional peptide library to identify IKKe phosphorylation substrates...
  34. Predictive algorithms for T cell epitope identification
    Soren Buus; Fiscal Year: 2009
    ..pressure liquid chromatography;mass spectrometry;phage-display libraries;and combinatorial synthetic peptide library screens...
  35. Direct Epitope Identification and Validation
    William Hildebrand; Fiscal Year: 2009
    ..pressure liquid chromatography;mass spectrometry;phage-display libraries;and combinatorial synthetic peptide library screens...
  36. Tropomyosin in the antiangiogenic activity of HKa
    Keith McCrae; Fiscal Year: 2007
    ..purification of chymotrypsin-digested tropomyosin on HKa-sepharose, as w ell as panning of a cyclic random peptide Library on HKa, led to tentative identification of HKa binding regions within tropomyosin...
  37. Peptides As Molecular Probes for the Detection and Differentiation of SUVs and LU
    LESLIE ANNE MORTON; Fiscal Year: 2013
    ..Identifying peptides that can sense curvature would be a novel approach. Chosen from a focused peptide library that were previously selected for the desired properties for curvature sensing, the MARCKS peptide provides a ..
  38. Large Scale Antibody and T Cell Epitope Discovery Program
    PH D FRELINGER; Fiscal Year: 2009
    ..pressure liquid chromatography;mass spectrometry;phage-display libraries;and combinatorial synthetic peptide library screens...
  39. Recognition and Catalysis of Phosphotyrosyl Proteins
    Dehua Pei; Fiscal Year: 2013
    ..The sequence specificity of an SH2 domain is systematically determined by screening a combinatorial peptide library and the consensus motif(s) is used to search the phosphoprotein databases to identify potential SH2-pY ..
  40. Listeria Immunotherapy for Pancreatic and Ovarian Cancer
    Thomas Dubensky; Fiscal Year: 2006
    ..We extended the use of the LLO peptide library to measure Lm cellular immunity in humans...
  41. Phagocyte Contact Response in Aspergillosis Immunity
    JAMES BURRITT; Fiscal Year: 2005
    ..fumigatus conidia and hyphal forms by phage-display peptide library analysis. Affinity selection of unique peptides will be carried out by probing the A...
  42. Technology to Optimize scFvs for Targeting Therapeutics
    MARK FEDERSPIEL; Fiscal Year: 2007
    ..of a variety of eukaryotic polypeptides including scFvs, and the efficient generation and selection of a peptide library in eukaryotic cells...
  43. Alpha2-antiplasmin polymorphism and a new plasma enzyme
    Patrick McKee; Fiscal Year: 2006
    ..Substrate specificity and subsite preferences of cFAP will be sought using a synthetic peptide library of sequences surrounding the Pro12-Asn13 bond cleaved by cFAP...
  44. Dendritic cell targeted hepatitis c virus immunotherapy
    Tyler Curiel; Fiscal Year: 2003
    ..we have generated peptides that bind specifically to LCs or interstitial DCs from a phage display peptide library. We hypothesize that targeting NS3 directly to DCs will increase the level and duration of specific immune ..
  45. Structural biology of cedar pollen allergy
    Terumi Midoro Horiuti; Fiscal Year: 2007
    ..will test the hypothesis are to: (1) Identify conformational IgE and IgG epitopes by probing a phage display peptide library. (2) Identify critical elements of linear and conformational epitopes by comparing structures of sensitizing ..
  46. TARGETED DELIVERY OF GENES TO ANGIOGENIC VASCULATURE
    Renata Pasqualini; Fiscal Year: 2004
    ..an in vivo selection system in which phage capable of homing to tumors are recovered from a phage display peptide library following intravenous administration. Using this strategy, we have isolated several tumor-homing phage...
  47. TOXOPLASMOSIS--INFECTION AND IMMUNE RESPONSE
    Jack Remington; Fiscal Year: 2001
    ..gondii acute phase antigens using a phage-display random peptide library. Our observation of unique antigens recognized by the fetus but not by their mothers will be further examined ..
  48. MAPPING VACULAR DIVERSITY OF HUMAN CANCER
    Wadih Arap; Fiscal Year: 2006
    ..home to specific vascular beds can be selected after intravenous administration of a phage display random peptide library. This work has uncovered a vascular address system that allows organ-specific targeting to normal blood ..
  49. Antimicrobial targets of intracellular cyclic peptides
    Ernesto Abel Santos; Fiscal Year: 2007
    ..For each potential target selected, the intracellular peptide library will be re-screened to detect all inhibitors of the isolated biological function...
  50. Combinatorial chemistry based approach to proteome exploration.
    Julia Lathrop; Fiscal Year: 2007
    ..balancing of protein concentrations in a sample using a solid-phase combinatorial peptide library (SPCL1) and eluting under non-denaturing conditions; B...
  51. STRUCTURE/FUNCTION OF EFF-2 KINASE
    ALEXEY RYAZANOV; Fiscal Year: 2003
    ..We will also study the mechanism of substrate recognition by eEF-2 kinase. An oriented peptide library will be screened to identify the consensus sequence recognized by eEF- 2 kinase...
  52. Double-Gated Selection of Ligands that Target Surface Markers of Differentiation
    David Larocca; Fiscal Year: 2009
    ..After optimizing the vector using the neural progenitor pathway, we will construct a large random peptide library and select it for cardiomyocyte targeting peptides...
  53. Peptide-grafting lens to prevent bacterial adhesion and biofilm formation
    YANLEI LIU; Fiscal Year: 2006
    ..Our preliminary data also demonstrates that 1-bead 1-compound (OBOC) combinatorial peptide library is a powerful and reliable approach to identify a single compound against multiple bacterial adhesions and ..
  54. Molecular Diversity in Bladder Cancer
    Wadih Arap; Fiscal Year: 2009
    ..that home selectively to different tissues are recovered after intravenous administration of a phage random peptide library. In earlier work, we uncovered a previously unrecognized address system that makes possible organ-specific ..
  55. Mechanism of Lnk function in cytokine receptor signaling
    Wei Tong; Fiscal Year: 2009
    ..Recombinant peptide library screens will establish Lnk SH2 domain binding motifs and mass spectrometry (MS) will identify Lnk SH2 domain ..
  56. HLA CLASS I PRESENTATION OF HIV PEPTIDES
    Margaret Smith; Fiscal Year: 1999
    ..Recombinant proteins will be used to screen a synthetic peptide library to determine allele-specific peptide binding motifs, and the resultant peptides will be synthesized and used ..
  57. Structural Studies of CR2 Interaction with EBVgp350
    Xiaojiang Chen; Fiscal Year: 2006
    ..At the same time, phage peptide library will be employed to screen for peptide ligands for gp350 that can specifically inhibit the binding of gp350 ..
  58. Identifying T cell ligands in sarcoidosis
    ANDREW FONTENOT; Fiscal Year: 2005
    ..This approach will utilize a baculovirus-generated HLA-DRB1*0301 peptide library in which SF9 insect cells will each express a different DR3/peptide complex...
  59. Interaction in vivo between HIV and Hepatitis B Virus
    Sharon Lewin; Fiscal Year: 2005
    ..This project includes the application of an overlapping peptide library designed to quantify HBV-specific T cell responses to all HBV proteins, in all HLA types and with infection ..
  60. FAB LIBRARIES TO DISCOVER VACCINE LEADS AGAINST HIV-1
    Jamie Scott; Fiscal Year: 2000
    ..Thus, when such Fabs are used to screen a peptide library, "immunogenic-mimic" peptides should be found that will both cross-react with the Fabs and elicit target-..
  61. INSULIN RECEPTOR STRUCTURE AND TURNOVER
    C Kahn; Fiscal Year: 1999
    ..and the cellular sites of receptor processing we will use in vitro assays with both the holoreceptor and a peptide library approach...
  62. Targeting of alpha v beta 6 integrin in oral cancer
    Julie Sutcliffe; Fiscal Year: 2005
    ..4-[19F]-fluorobenzoyl will be incorporated into the peptide library as an approach to transition the identified sequences into a radiolabeled peptide for imaging using MicroPET ..