rab gtp binding proteins

Summary

Summary: A large family of MONOMERIC GTP-BINDING PROTEINS that play a key role in cellular secretory and endocytic pathways. EC 3.6.1.-.

Top Publications

  1. ncbi Identification of an organelle receptor for myosin-Va
    Xufeng S Wu
    Laboratory of Cell Biology, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Nat Cell Biol 4:271-8. 2002
  2. ncbi Role for Rab7 in maturation of late autophagic vacuoles
    Stefanie Jäger
    Institute of Biochemistry, University of Kiel, Olshausenstr 40, 24098 Kiel, Germany
    J Cell Sci 117:4837-48. 2004
  3. ncbi Rab GTPases as coordinators of vesicle traffic
    Harald Stenmark
    Department of Biochemistry, Institute for Cancer Research, The Norwegian Radium Hospital, University of Oslo, Montebello, N 0310 Oslo, Norway
    Nat Rev Mol Cell Biol 10:513-25. 2009
  4. ncbi Rab proteins as membrane organizers
    M Zerial
    Max Planck Institute of Molecular Cell Biology and Genetics, c o EMBL, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    Nat Rev Mol Cell Biol 2:107-17. 2001
  5. ncbi A core complex of BBS proteins cooperates with the GTPase Rab8 to promote ciliary membrane biogenesis
    Maxence V Nachury
    Genentech, Inc, South San Francisco, CA 94080, USA
    Cell 129:1201-13. 2007
  6. ncbi Endocytic recycling
    Frederick R Maxfield
    Department of Biochemistry, Weill Medical College of Cornell University, 1300 York Avenue, New York, New York 10021, USA
    Nat Rev Mol Cell Biol 5:121-32. 2004
  7. ncbi Rab conversion as a mechanism of progression from early to late endosomes
    Jochen Rink
    Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307 Dresden, Germany
    Cell 122:735-49. 2005
  8. pmc Rab7: a key to lysosome biogenesis
    C Bucci
    Structural Cell Biology Unit, Department of Medical Anatomy, The Panum Institute, University of Copenhagen, DK 2200 Copenhagen, Denmark
    Mol Biol Cell 11:467-80. 2000
  9. ncbi Evolution of the Rab family of small GTP-binding proteins
    J B Pereira-Leal
    Cell and Molecular Biology Section, Division of Biomedical Sciences, Faculty of Medicine, Imperial College, London, SW7 2AZ, UK
    J Mol Biol 313:889-901. 2001
  10. ncbi TBC-domain GAPs for Rab GTPases accelerate GTP hydrolysis by a dual-finger mechanism
    Xiaojing Pan
    Program in Molecular Medicine and Department of Biochemistry and Molecular Pharmacology, UMass Medical School, Two Biotech, 373 Plantation Street, Worcester, Massachusetts 01605, USA
    Nature 442:303-6. 2006

Research Grants

  1. RAB GTP BINDING PROTEINS AND CELL GROWTH
    WILLIAM MALTESE; Fiscal Year: 2005
  2. Characterization of a novel nuclear Rab protein
    Ying Huang; Fiscal Year: 2007
  3. Molecular Mechanisms Controlling Endocytic Recycling
    STEVEN H CAPLAN; Fiscal Year: 2013
  4. INTRACELLULAR TRANSPORT--THE MANNOSE-PHOSPHATE RECEPTOR
    Suzanne Pfeffer; Fiscal Year: 2008
  5. LOCALIZATION OF THE GOLGI COMPLEX
    Suzanne Pfeffer; Fiscal Year: 2001
  6. Molecular Analysis of the CCC185 Golgin
    Suzanne R Pfeffer; Fiscal Year: 2010
  7. Renal Epithelial Sodium Channels and Syntaxins
    Sunil Saxena; Fiscal Year: 2006
  8. Regulation of polarized cell growth by GTPases
    Hay Oak Park; Fiscal Year: 2009
  9. SPATIAL CONTROL OF CELL POLARITY DURING YEAST BUDDING
    Hay Oak Park; Fiscal Year: 2003
  10. Pseudomonas in Cystic Fibrosis-Regulation of Mucoidy
    Vojo Deretic; Fiscal Year: 2006

Detail Information

Publications279 found, 100 shown here

  1. ncbi Identification of an organelle receptor for myosin-Va
    Xufeng S Wu
    Laboratory of Cell Biology, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Nat Cell Biol 4:271-8. 2002
    ....
  2. ncbi Role for Rab7 in maturation of late autophagic vacuoles
    Stefanie Jäger
    Institute of Biochemistry, University of Kiel, Olshausenstr 40, 24098 Kiel, Germany
    J Cell Sci 117:4837-48. 2004
    ..In conclusion, our results showed a role for Rab7 in the final maturation of late autophagic vacuoles...
  3. ncbi Rab GTPases as coordinators of vesicle traffic
    Harald Stenmark
    Department of Biochemistry, Institute for Cancer Research, The Norwegian Radium Hospital, University of Oslo, Montebello, N 0310 Oslo, Norway
    Nat Rev Mol Cell Biol 10:513-25. 2009
    ..Functional impairments of Rab pathways are associated with diseases, such as immunodeficiencies, cancer and neurological disorders...
  4. ncbi Rab proteins as membrane organizers
    M Zerial
    Max Planck Institute of Molecular Cell Biology and Genetics, c o EMBL, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    Nat Rev Mol Cell Biol 2:107-17. 2001
    ..These molecules are highly compartmentalized in organelle membranes, making them excellent candidates for determining transport specificity and organelle identity...
  5. ncbi A core complex of BBS proteins cooperates with the GTPase Rab8 to promote ciliary membrane biogenesis
    Maxence V Nachury
    Genentech, Inc, South San Francisco, CA 94080, USA
    Cell 129:1201-13. 2007
    ..Conversely, preventing Rab8(GTP) production blocks ciliation in cells and yields characteristic BBS phenotypes in zebrafish. Our data reveal that BBS may be caused by defects in vesicular transport to the cilium...
  6. ncbi Endocytic recycling
    Frederick R Maxfield
    Department of Biochemistry, Weill Medical College of Cornell University, 1300 York Avenue, New York, New York 10021, USA
    Nat Rev Mol Cell Biol 5:121-32. 2004
    ..The molecular basis for many of these sorting processes is only partly understood...
  7. ncbi Rab conversion as a mechanism of progression from early to late endosomes
    Jochen Rink
    Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307 Dresden, Germany
    Cell 122:735-49. 2005
    ..Our results reveal unexpected dynamics of Rab domains and suggest Rab conversion as the mechanism of cargo progression between early and late endosomes...
  8. pmc Rab7: a key to lysosome biogenesis
    C Bucci
    Structural Cell Biology Unit, Department of Medical Anatomy, The Panum Institute, University of Copenhagen, DK 2200 Copenhagen, Denmark
    Mol Biol Cell 11:467-80. 2000
    ..These data demonstrate for the first time that Rab7, controlling aggregation and fusion of late endocytic structures/lysosomes, is essential for maintenance of the perinuclear lysosome compartment...
  9. ncbi Evolution of the Rab family of small GTP-binding proteins
    J B Pereira-Leal
    Cell and Molecular Biology Section, Division of Biomedical Sciences, Faculty of Medicine, Imperial College, London, SW7 2AZ, UK
    J Mol Biol 313:889-901. 2001
    ..Rab functional groups can help the understanding of the functional evolution of the Rab family in particular and vesicular transport in general, and may be used to predict general functions for novel Rab sequences...
  10. ncbi TBC-domain GAPs for Rab GTPases accelerate GTP hydrolysis by a dual-finger mechanism
    Xiaojing Pan
    Program in Molecular Medicine and Department of Biochemistry and Molecular Pharmacology, UMass Medical School, Two Biotech, 373 Plantation Street, Worcester, Massachusetts 01605, USA
    Nature 442:303-6. 2006
    ..Strong conservation of both catalytic fingers indicates that most TBC-domain GAPs may accelerate GTP hydrolysis by a similar dual-finger mechanism...
  11. ncbi Targeting Rab GTPases to distinct membrane compartments
    Suzanne Pfeffer
    Department of Biochemistry, Stanford University School of Medicine, Stanford, California 94305 5307, USA
    Nat Rev Mol Cell Biol 5:886-96. 2004
    ..The further discovery of an integral membrane protein that can dissociate prenylated Rab proteins from GDP-dissociation inhibitor gives new insights into the mechanisms of Rab localization...
  12. pmc Rab7 associates with early endosomes to mediate sorting and transport of Semliki forest virus to late endosomes
    Andreas Vonderheit
    Institute of Biochemistry, Swiss Federal Institute of Technology, Zurich, Switzerland
    PLoS Biol 3:e233. 2005
    ..We concluded that Rab7 is recruited to early endosomes, where it forms distinct domains that mediate cargo sorting as well as the formation of late-endosome-targeted transport vesicles...
  13. pmc Thirty-one flavors of Drosophila rab proteins
    Jun Zhang
    Department of Developmental Biology, Howard Hughes Medical Institute, Stanford University School of Medicine, Stanford, California 94305, USA
    Genetics 176:1307-22. 2007
    ..The high evolutionary conservation and low redundancy of Drosophila Rab proteins make these transgenic lines a useful tool kit for investigating Rab functions in vivo...
  14. pmc Mutations in the small GTP-ase late endosomal protein RAB7 cause Charcot-Marie-Tooth type 2B neuropathy
    Kristien Verhoeven
    Department of Molecular Genetics, Flanders Interuniversity Institute for Biotechnology, Born Bunge Foundation, University of Antwerp, Antwerp, Belgium
    Am J Hum Genet 72:722-7. 2003
    ..The alignment of RAB7 orthologs shows that both missense mutations target highly conserved amino acid residues. RAB7 is ubiquitously expressed, and we found expression in sensory and motor neurons...
  15. ncbi Rab GTPases at a glance
    Samantha L Schwartz
    Department of Pathology MSC08 4640, University of New Mexico, 2325 Camino de Salud NE, CRF225, Albuquerque, NM 87131, USA
    J Cell Sci 120:3905-10. 2007
  16. ncbi Rab11-FIP2 functions in transferrin recycling and associates with endosomal membranes via its COOH-terminal domain
    Andrew J Lindsay
    Cell and Molecular Biology Laboratory, Department of Biochemistry, University College Cork, Lee Maltings, Prospect Row, Cork, Ireland
    J Biol Chem 277:27193-9. 2002
    ..This phenotype cannot be reversed by excess wild-type Rab11, or dominant-positive Rab11 (Rab11Q70L), suggesting that Rab11-FIP2 functions downstream of Rab11 in endosomal trafficking...
  17. pmc A Rab GAP cascade defines the boundary between two Rab GTPases on the secretory pathway
    Félix E Rivera-Molina
    Department of Cell Biology, Yale University School of Medicine, New Haven, CT 06520, USA
    Proc Natl Acad Sci U S A 106:14408-13. 2009
    ..GEF cascades working in concert with counter-current GAP cascades could generate a programmed series of Rab conversions responsible for regulating the choreography of membrane traffic...
  18. pmc Phagosomes fuse with late endosomes and/or lysosomes by extension of membrane protrusions along microtubules: role of Rab7 and RILP
    Rene E Harrison
    Division of Cell Biology, The Hospital for Sick Children, 555 University Avenue, Toronto, Ontario, Canada M5G 1X8
    Mol Cell Biol 23:6494-506. 2003
    ....
  19. pmc Interactions between EHD proteins and Rab11-FIP2: a role for EHD3 in early endosomal transport
    Naava Naslavsky
    Department of Biochemistry and Molecular Biology and Eppley Cancer Center, University of Nebraska Medical Center, Omaha, NE 68198 5870, USA
    Mol Biol Cell 17:163-77. 2006
    ..The results presented herein promote a coordinated role for EHD proteins and Rab11-FIP2 in mediating endocytic recycling and provide evidence for the function of EHD3 in early endosome to ERC transport...
  20. ncbi Functional characterization of Rab7 mutant proteins associated with Charcot-Marie-Tooth type 2B disease
    Maria Rita Spinosa
    Dipartimento di Scienze e Tecnologie Biologiche ed Ambientali, Universita del Salento, 73100 Lecce, Italy
    J Neurosci 28:1640-8. 2008
    ..Altogether, these data demonstrate that all tested CMT2B-associated Rab7 mutations are mechanistically similar, suggesting that activated forms of the Rab7 are responsible for CMT2B disease...
  21. pmc Modulation of Rab5 and Rab7 recruitment to phagosomes by phosphatidylinositol 3-kinase
    Otilia V Vieira
    Cell Biology Program, Hospital for Sick Children and Department of Biochemistry, University of Toronto, Ontario M5G 1X8, Canada
    Mol Cell Biol 23:2501-14. 2003
    ..We propose that Rab5 activates both PI3K-dependent and PI3K-independent effectors that act in parallel to promote phagosome maturation...
  22. pmc Genetic analysis of lysosomal trafficking in Caenorhabditis elegans
    Greg J Hermann
    Department of Biology, Lewis and Clark College, Portland, OR 97219, USA
    Mol Biol Cell 16:3273-88. 2005
    ..The glo mutants thus provide a simple model system for the analysis of lysosome-related organelle biogenesis in animal cells...
  23. ncbi Active Rab11 and functional recycling endosome are required for E-cadherin trafficking and lumen formation during epithelial morphogenesis
    Marion Desclozeaux
    Institute for Molecular Bioscience, University of Queensland, Brisbane 4072 Queensland, Australia
    Am J Physiol Cell Physiol 295:C545-56. 2008
    ..This final effect demonstrated an early and critical interdependence of Rab11 and the RE for E-cadherin targeting, apical membrane formation, and cell polarity in cysts...
  24. ncbi REP-2, a Rab escort protein encoded by the choroideremia-like gene
    F P Cremers
    Department of Molecular Genetics, University of Texas Southwestern Medical Center at Dallas, Texas 75235 9046
    J Biol Chem 269:2111-7. 1994
    ..In the retina, REP-2 may be only partially effective, leading eventually to retinal degeneration and blindness...
  25. pmc Ligands for clathrin-mediated endocytosis are differentially sorted into distinct populations of early endosomes
    Melike Lakadamyali
    Howard Hughes Medical Institute, Harvard University, Cambridge, MA 02138, USA
    Cell 124:997-1009. 2006
    ..This pre-early endosome sorting process begins at clathrin-coated vesicles, depends on microtubule-dependent motility, and appears to involve endocytic adaptors...
  26. ncbi Identification and characterization of mouse Rab32 by mRNA and protein expression analysis
    Karine A Cohen-Solal
    Ernest Mario School of Pharmacy, Rutgers, The State University of New Jersey, Piscataway, NJ 08854, USA
    Biochim Biophys Acta 1651:68-75. 2003
    ..Based on these data, we propose the inclusion of Rab32 to the so-called melanocyte/platelet family of Rab proteins...
  27. pmc Rab11 is required for trans-golgi network-to-plasma membrane transport and a preferential target for GDP dissociation inhibitor
    W Chen
    Department of Biochemistry, Molecular Biology, and Cell Biology, Northwestern University, Evanston, Illinois 60208 3500, USA
    Mol Biol Cell 9:3241-57. 1998
    ..These data show that functional rab11 is critical for the export of a basolateral marker but not an apical marker from the trans-Golgi network and pinpoint rab11 as a sensitive target for inhibition by excess GDI...
  28. ncbi Linking albinism and immunity: the secrets of secretory lysosomes
    Jane Stinchcombe
    Sir William Dunn School of Pathology, South Parks Road, Oxford OX1 3RE, UK
    Science 305:55-9. 2004
    ....
  29. pmc Myosin vb is associated with plasma membrane recycling systems
    L A Lapierre
    Departments of Medicine, Surgery, and Cellular Biology and Anatomy, Institute of Molecular Medicine and Genetics, Medical College of Georgia and the Augusta VA Medical Center, Augusta, Georgia 30912, USA
    Mol Biol Cell 12:1843-57. 2001
    ..The dominant negative effects of the myosin Vb tail chimera indicate that this unconventional myosin is required for transit out of plasma membrane recycling systems...
  30. ncbi Trafficking through Rab11 endosomes is required for cellularization during Drosophila embryogenesis
    Anne Pelissier
    Laboratoire de Genetique et de Physiologie du Developpement, Institut de Biologie du Developpement de Marseille, France
    Curr Biol 13:1848-57. 2003
    ..In Drosophila, the process is specialized and called cellularization. The trafficking pathways that underlie this process and that are responsible for the mobilization of membrane pools, however, remain poorly understood...
  31. ncbi Mutations in RAB27A cause Griscelli syndrome associated with haemophagocytic syndrome
    G Menasche
    1 Unité de Recherches sur le Dévelopment Normal et Pathologique du Système Immunitaire INSERM U429, Paris, France
    Nat Genet 25:173-6. 2000
    ..RAB27A appears to be a key effector of cytotoxic granule exocytosis, a pathway essential for immune homeostasis...
  32. pmc Myosin Vb interacts with Rab8a on a tubular network containing EHD1 and EHD3
    Joseph T Roland
    Department of Surgery, Vanderbilt University School of Medicine, Nashville, TN 37232 2733, USA
    Mol Biol Cell 18:2828-37. 2007
    ..Additionally, dynamic live cell imaging demonstrates distinct pathways for Rab11a and Rab8a vesicle trafficking. These findings indicate that Rab8a and Rab11a define different recycling pathways that both use Myosin Vb...
  33. pmc Induction of salt and osmotic stress tolerance by overexpression of an intracellular vesicle trafficking protein AtRab7 (AtRabG3e)
    Alexander Mazel
    Department of Plant Sciences, Institute of Life Sciences, The Hebrew University of Jerusalem, Givat Ram, Jerusalem 91904, Israel
    Plant Physiol 134:118-28. 2004
    ..These results imply that vesicle trafficking plays an important role in plant adaptation to stress, beyond the housekeeping function in intracellular vesicle trafficking...
  34. ncbi Slac2-a/melanophilin, the missing link between Rab27 and myosin Va: implications of a tripartite protein complex for melanosome transport
    Mitsunori Fukuda
    Laboratory for Developmental Neurobiology, Brain Science Institute, RIKEN The Institute of Physical and Chemical Research, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
    J Biol Chem 277:12432-6. 2002
    ..The discovery that myosin Va indirectly recognizes its cargo through Slac2-a, a novel Rab27A/B effector, should shed light on molecular recognition of its specific cargo by class V myosin...
  35. ncbi Insulin-stimulated phosphorylation of a Rab GTPase-activating protein regulates GLUT4 translocation
    Hiroyuki Sano
    Department of Biochemistry, Dartmouth Medical School, Hanover, New Hampshire 03755, USA
    J Biol Chem 278:14599-602. 2003
    ..These findings strongly indicate that insulin-stimulated phosphorylation of AS160 is required for GLUT4 translocation and that this phosphorylation signals translocation through inactivation of the Rab GAP function...
  36. ncbi Lysosome-associated small Rab GTPase Rab7b negatively regulates TLR4 signaling in macrophages by promoting lysosomal degradation of TLR4
    Yuzhen Wang
    Institute of Immunology, Zhejiang University, 388 Yuhangtang Road, Hangzhou 310058, P R China
    Blood 110:962-71. 2007
    ..Our findings suggest that Rab7b is a negative regulator of TLR4 signaling, potentially by promoting the translocation of TLR4 into lysosomes for degradation...
  37. ncbi C. elegans Rab GTPase 2 is required for the degradation of apoptotic cells
    Qun Lu
    College of Biological Sciences, China Agricultural University, Beijing 100094, China
    Development 135:1069-80. 2008
    ..UNC-108 co-localizes with RAB-5, RAB-7 and LMP-1 to the phagosome and promotes cell corpse degradation, possibly by mediating phagosome maturation...
  38. pmc The Rab GTPase family
    H Stenmark
    Department of Biochemistry, Norwegian Radium Hospital, Montebello, N 0310 Oslo, Norway
    Genome Biol 2:REVIEWS3007. 2001
    ..In the GTP-bound form, the Rab GTPases recruit specific sets of effector proteins onto membranes. Through their effectors, Rab GTPases regulate vesicle formation, actin- and tubulin-dependent vesicle movement, and membrane fusion...
  39. pmc Rabs and their effectors: achieving specificity in membrane traffic
    Bianka L Grosshans
    Department of Cell Biology, Yale University School of Medicine, New Haven, CT 06520, USA
    Proc Natl Acad Sci U S A 103:11821-7. 2006
    ..Additionally, Rab cascades and Rab conversions appear to confer directionality to membrane traffic and couple each stage of traffic with the next along the pathway...
  40. pmc Initial docking of ER-derived vesicles requires Uso1p and Ypt1p but is independent of SNARE proteins
    X Cao
    Department of Biochemistry, Dartmouth Medical School, Hanover, NH 03755, USA
    EMBO J 17:2156-65. 1998
    ..We propose that an initial vesicle docking event of ER-derived vesicles, termed tethering, depends on Uso1p and Ypt1p but is independent of SNARE proteins...
  41. ncbi Tropomyosin-containing actin cables direct the Myo2p-dependent polarized delivery of secretory vesicles in budding yeast
    D W Pruyne
    Section of Biochemistry, Molecular and Cell Biology, Cornell University, Ithaca, New York 14853, USA
    J Cell Biol 143:1931-45. 1998
    ..Therefore, actin cables respond to polarity cues independently of the overall distribution of cortical patches and are able to directly target the Myo2p-dependent delivery of secretory vesicles and polarization of growth...
  42. ncbi Regulation of secretory vesicle traffic by Rab small GTPases
    M Fukuda
    Laboratory of Membrane Trafficking Mechanisms, Department of Developmental Biology and Neurosciences, Graduate School of Life Sciences, Tohoku University, Aobayama, Aoba ku, Sendai, Miyagi 980 8578, Japan
    Cell Mol Life Sci 65:2801-13. 2008
    ..In this article I review the current literature on the function of Rab isoforms and their effectors in regulated secretory vesicle traffic...
  43. pmc Ypt32 recruits the Sec4p guanine nucleotide exchange factor, Sec2p, to secretory vesicles; evidence for a Rab cascade in yeast
    Darinel Ortiz
    Department of Cell Biology, Yale University School of Medicine, New Haven, CT 06510, USA
    J Cell Biol 157:1005-15. 2002
    ..We propose that Ypt32p and Sec4p act as part of a signaling cascade in which Ypt32p recruits Sec2p to secretory vesicles; once on the vesicle, Sec2p activates Sec4p, enabling the polarized transport of vesicles to the plasma membrane...
  44. ncbi Stimulation-dependent recycling of integrin beta1 regulated by ARF6 and Rab11
    Aimee M Powelka
    Brigham and Women s Hospital, and Department of Medicine, Harvard Medical School, Boston, MA 02115, USA
    Traffic 5:20-36. 2004
    ..Consistent with these observations being relevant for cell motility, mutant forms of ARF6 that affect either actin rearrangement or recycling inhibit the motility of a breast cancer cell line...
  45. pmc Rab22a regulates the recycling of membrane proteins internalized independently of clathrin
    Roberto Weigert
    Laboratory of Cell Biology, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892 8017, USA
    Mol Biol Cell 15:3758-70. 2004
    ..Dominant negative mutant of Rab11a also inhibited recycling of MHCI but not the formation of recycling tubules, suggesting that Rab22a and Rab11a might coordinate different steps of MHCI recycling...
  46. pmc Functional redundancy of Rab27 proteins and the pathogenesis of Griscelli syndrome
    Duarte C Barral
    Cell and Molecular Biology, Division of Biomedical Sciences, Faculty of Medicine, Imperial College, London, United Kingdom
    J Clin Invest 110:247-57. 2002
    ..Our data suggest that Rab27b is functionally redundant with Rab27a and that the pathogenesis of GS is determined by the relative expression of Rab27a and Rab27b in specialized cell types...
  47. ncbi Controlling the location and activation of Rab GTPases
    Miguel C Seabra
    Cell and Molecular Biology Section, Division of Biomedical Sciences, Faculty of Medicine, Imperial College London, London SW7 2AZ, UK
    Curr Opin Cell Biol 16:451-7. 2004
    ..The diversity of cellular processes regulated by Rab family members is made possible, not only by the wide range of effectors they recruit, but also by the different mechanisms regulating their own targeting and activation...
  48. ncbi Charcot-Marie-Tooth disease due to a de novo mutation of the RAB7 gene
    F Meggouh
    Neurogenetics Department, AMC, Meibergdreef 9, 1105 AZ Amsterdam, The Netherlands
    Neurology 67:1476-8. 2006
    ..471G>C, p.Lys157Asn missense mutation. This observation strongly supports the hypothesis that RAB7 mutations are responsible for CMT2B...
  49. pmc The Rab27a-binding protein, JFC1, regulates androgen-dependent secretion of prostate-specific antigen and prostatic-specific acid phosphatase
    Jennifer L Johnson
    Division of Biochemistry, Department of Molecular and Experimental Medicine, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Biochem J 391:699-710. 2005
    ..In conclusion, we present evidence that JFC1 differentially regulates the secretion of PSAP and PSA, and that Rab27a and PI3K play a central role in the exocytosis of prostate-specific markers...
  50. ncbi Sec18p (NSF)-driven release of Sec17p (alpha-SNAP) can precede docking and fusion of yeast vacuoles
    A Mayer
    Department of Biochemistry, Dartmouth Medical School, Hanover, New Hampshire, 03755 3844 USA
    Cell 85:83-94. 1996
    ..Sec17p and Sec18p thus may function in a predocking stage of the reaction, rather than in bilayer fusion per se...
  51. ncbi Bicaudal-D regulates COPI-independent Golgi-ER transport by recruiting the dynein-dynactin motor complex
    Theodoros Matanis
    Department of Experimental Tumorbiology, University of Muenster, Badestrasse 9, D 48149 Muenster, Germany
    Nat Cell Biol 4:986-92. 2002
    ..These data suggest that coordinated action between Rab6a, BICD and the dynein-dynactin complex controls COPI-independent Golgi-ER transport...
  52. pmc Specific Rab GTPase-activating proteins define the Shiga toxin and epidermal growth factor uptake pathways
    Evelyn Fuchs
    Department of Cell Biology, Max Planck Institute of Biochemistry, Martinsried 82152, Germany
    J Cell Biol 177:1133-43. 2007
    ..These results suggest that Shiga toxin trafficking to the Golgi is a multistep process controlled by several Rab GAPs and their target Rabs and that this process is discrete from ligand-induced EGF receptor trafficking...
  53. ncbi The Rab-interacting lysosomal protein, a Rab7 and Rab34 effector, is capable of self-interaction
    Anna Maria Rosaria Colucci
    Dipartimento di Scienze e Tecnologie Biologiche ed Ambientali, Universita degli Studi di Lecce, Via Monteroni, 73100 Lecce, Italy
    Biochem Biophys Res Commun 334:128-33. 2005
    ..The data together indicate that RILP, as already demonstrated for several other Rab effector proteins, is capable of self-association, thus probably forming a homo-dimer...
  54. pmc A unique region of RILP distinguishes it from its related proteins in its regulation of lysosomal morphology and interaction with Rab7 and Rab34
    Tuanlao Wang
    Membrane Biology Laboratory, Institute of Molecular and Cell Biology, Singapore 117609, Singapore
    Mol Biol Cell 15:815-26. 2004
    ..These results define a unique region in RILP responsible for its specific role in regulating lysosomal morphology as well as in its interaction with Rab7 and Rab34...
  55. pmc The TRAPP complex is a nucleotide exchanger for Ypt1 and Ypt31/32
    S Jones
    Department of Biological Sciences, Laboratory for Molecular Biology, University of Illinois at Chicago, Chicago, Illinois 60607, USA
    Mol Biol Cell 11:4403-11. 2000
    ..These data suggest the exciting possibility that a GEF complex common to Ypt1 and Ypt31/32 might coordinate the function of these GTPases in entry into and exit from the Golgi...
  56. ncbi The roles of Rab27 and its effectors in the regulated secretory pathways
    Tetsuro Izumi
    Department of Molecular Medicine, Institute for Molecular and Cellular Regulation, Gunma University, 3 39 15 Showa Machi, Maebashi, Gunma 371 8512, Japan
    Cell Struct Funct 28:465-74. 2003
    ..These findings suggest that the Rab27 subfamily regulates various exocytotic pathways using multiple organelle-specific effector proteins...
  57. ncbi Rab7b, a novel lysosome-associated small GTPase, is involved in monocytic differentiation of human acute promyelocytic leukemia cells
    Mingjin Yang
    Institute of Immunology, Zhejiang University, 353 Yanan Road, Hangzhou 310031, PR China
    Biochem Biophys Res Commun 318:792-9. 2004
    ..Our data suggest that Rab7b is a lysosome-localized monocytic cell-specific small GTPase, and is involved in PMA-induced APL cell differentiation and possibly in regulation of monocyte functions...
  58. pmc Regulation of anterograde transport of adrenergic and angiotensin II receptors by Rab2 and Rab6 GTPases
    Chunmin Dong
    Department of Pharmacology and Experimental Therapeutics, Louisiana State University, Health Sciences Center, New Orleans, LA 70112, USA
    Cell Signal 19:2388-99. 2007
    ..These data also provide the first evidence indicating that Rab6-coordinated retrograde transport selectively modulates intracellular trafficking and signaling of GPCRs...
  59. ncbi The RAB25 small GTPase determines aggressiveness of ovarian and breast cancers
    Kwai Wa Cheng
    Department of Molecular Therapeutics, University of Texas MD Anderson Cancer Center, Houston, Texas 77054, USA
    Nat Med 10:1251-6. 2004
    ..Overall, these studies implicate RAB25, and thus the RAB family of small G proteins, in aggressiveness of epithelial cancers...
  60. ncbi Secretory cytotoxic granule maturation and exocytosis require the effector protein hMunc13-4
    Mickaël M Ménager
    Institut National de la Santé et de la Recherche Médicale Unité 768, Laboratoire du Développement Normal et Pathologique du Système Immunitaire, Paris F 75015 France
    Nat Immunol 8:257-67. 2007
    ..Our data provide insight into the regulation of the generation and release of cytotoxic granules by effector cytotoxic T lymphocytes and natural killer cells...
  61. pmc A retromerlike complex is a novel Rab7 effector that is involved in the transport of the virulence factor cysteine protease in the enteric protozoan parasite Entamoeba histolytica
    Kumiko Nakada-Tsukui
    Department of Parasitology, Gunma University Graduate School of Medicine, Maebashi 371 8511, Japan
    Mol Biol Cell 16:5294-303. 2005
    ..The reduced CP activity was restored by the coexpression of EhVps26, implying that the EhRab7A-mediated transport of CP to phagosomes is regulated by the retromerlike complex...
  62. pmc Rab8 regulates basolateral secretory, but not recycling, traffic at the recycling endosome
    Lauren Henry
    Department of Pharmacology, University of Iowa Carver College of Medicine, Iowa City, IA 52242, USA
    Mol Biol Cell 19:2059-68. 2008
    ..The data are consistent with a model in which Rab8 functions in regulating the delivery of TGN-derived cargo to REs...
  63. pmc The vacuolar kinase Yck3 maintains organelle fragmentation by regulating the HOPS tethering complex
    Tracy J LaGrassa
    Biochemie Zentrum der Universitat Heidelberg, 69120 Heidelberg, Germany
    J Cell Biol 168:401-14. 2005
    ..Because Yck3 also promotes efficient vacuole inheritance, we propose that tethering complex phosphorylation is a part of a general, switch-like mechanism for driving changes in organelle architecture...
  64. pmc Phagosome maturation: going through the acid test
    Jason M Kinchen
    Beirne B Carter Center for Immunology Research and the Department of Microbiology, University of Virginia, Charlottesville, Virginia 22902, USA
    Nat Rev Mol Cell Biol 9:781-95. 2008
    ..This suggests that a machinery that is distinct from receptor-mediated endocytosis is used in phagosome maturation...
  65. ncbi Varp is a Rab21 guanine nucleotide exchange factor and regulates endosome dynamics
    Xinjun Zhang
    Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China
    J Cell Sci 119:1053-62. 2006
    ..These results suggest that Varp is a potential Rab21 guanine nucleotide exchange factor and might regulate endosome dynamics in vivo...
  66. pmc The unconventional myosin-VIIa associates with lysosomes
    Lily E Soni
    Section of Cell and Developmental Biology, Division of Biological Sciences, University of California at San Diego, La Jolla, CA 92093 0368, USA
    Cell Motil Cytoskeleton 62:13-26. 2005
    ..Myosin-VIIa copurified with lysosomes on density gradients, and fractionation and extraction experiments suggested that it was tightly associated with the lysosome surface. These studies suggest that myosin-VIIa is a lysosome motor...
  67. ncbi Screening for target Rabs of TBC (Tre-2/Bub2/Cdc16) domain-containing proteins based on their Rab-binding activity
    Takashi Itoh
    Fukuda Initiative Research Unit, RIKEN, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
    Genes Cells 11:1023-37. 2006
    ....
  68. ncbi Regulated vacuole fusion and fission in Schizosaccharomyces pombe: an osmotic response dependent on MAP kinases
    N Bone
    School of Biological Sciences, University of Sussex, Falmer, Brighton, BN1 9QG, UK
    Curr Biol 8:135-44. 1998
    ..We have investigated the involvement of S. pombe MAP kinase pathways in signalling a newly described response to osmotic stress - that of vacuole fusion and fission...
  69. pmc TIP47 is a key effector for Rab9 localization
    Dikran Aivazian
    Department of Biochemistry, Stanford University School of Medicine, Stanford, CA 94305, USA
    J Cell Biol 173:917-26. 2006
    ..These data confirm the importance of effector interactions for Rab9 localization, and support a model in which effector proteins rely on Rabs as much as Rabs rely on effectors to achieve their correct steady state localizations...
  70. ncbi Divergent functions of neuronal Rab11b in Ca2+-regulated versus constitutive exocytosis
    Mikhail V Khvotchev
    Center for Basic Neuroscience, Department of Molecular Genetics, and Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas, Texas 75390 9111, USA
    J Neurosci 23:10531-9. 2003
    ....
  71. pmc Polarized growth controls cell shape and bipolar bud site selection in Saccharomyces cerevisiae
    Y J Sheu
    Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, Connecticut 06520 8103, USA
    Mol Cell Biol 20:5235-47. 2000
    ..We propose that apical growth and repolarization at the site of cytokinesis are crucial for establishing spatial cues used by diploid yeast cells to position division planes...
  72. pmc TRAPP stimulates guanine nucleotide exchange on Ypt1p
    W Wang
    Howard Hughes Medical Institute and the Department of Cell Biology Yale University School of Medicine, New Haven, Connecticut 06519 1418, USA
    J Cell Biol 151:289-96. 2000
    ..Furthermore, the purified TRAPP complex accelerates nucleotide exchange on Ypt1p. Our findings imply that Ypt1p, which is present on ER-to-Golgi transport vesicles, is activated at the Golgi once it interacts with TRAPP...
  73. ncbi A family of Rab27-binding proteins. Melanophilin links Rab27a and myosin Va function in melanosome transport
    Molly Strom
    Department of Cell and Molecular Biology, Division of Biomedical Sciences, Faculty of Medicine, Imperial College, London SW7 2AZ, United Kingdom
    J Biol Chem 277:25423-30. 2002
    ..Thus, melanophilin can associate simultaneously with activated Rab27a and myosin Va via distinct regions, and serve as a linker between these proteins...
  74. pmc Sequential action of two GTPases to promote vacuole docking and fusion
    G Eitzen
    Department of Biochemistry, Dartmouth Medical School, 7200 Vail Building, Hanover, NH 03755 3844, USA
    EMBO J 19:6713-20. 2000
    ..Thus, two GTPases sequentially govern vacuole docking and fusion...
  75. ncbi A small GTPase, human Rab32, is required for the formation of autophagic vacuoles under basal conditions
    Yuko Hirota
    Division of Pharmaceutical Cell Biology, Graduate School of Pharmaceutical Sciences, Kyushu University, 3 1 1 Maidashi, Fukuoka 812 8582, Japan
    Cell Mol Life Sci 66:2913-32. 2009
    ..We postulate that Rab32 facilitates the formation of autophagic vacuoles whose membranes are derived from the ER and regulates the clearance of aggregated proteins by autophagy...
  76. pmc The Rho GTPase Rho3 has a direct role in exocytosis that is distinct from its role in actin polarity
    J E Adamo
    Department of Cell Biology, Cell Biology, and Genetics, Weill Medical College of Cornell University, New York, New York 10021, USA
    Mol Biol Cell 10:4121-33. 1999
    ..These data suggest that Rho3 acts as a key regulator of cell polarity and exocytosis, coordinating several distinct events for delivery of proteins to specific sites on the cell surface...
  77. pmc Rab and Arl GTPase family members cooperate in the localization of the golgin GCC185
    Alondra Schweizer Burguete
    Department of Biochemistry, Stanford University School of Medicine, Stanford, CA 94305, USA
    Cell 132:286-98. 2008
    ....
  78. ncbi Rab6 regulates transport and targeting of exocytotic carriers
    Ilya Grigoriev
    Department of Cell Biology, Erasmus Medical Center, 3000 CA Rotterdam, The Netherlands
    Dev Cell 13:305-14. 2007
    ..Our data demonstrate that although Rab6 is not essential for secretion, it controls the organization of exocytosis within the cellular space...
  79. ncbi Protrudin induces neurite formation by directional membrane trafficking
    Michiko Shirane
    Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka, Fukuoka 812 8582, Japan
    Science 314:818-21. 2006
    ..Thus, protrudin regulates Rab11-dependent membrane recycling to promote the directional membrane trafficking required for neurite formation...
  80. pmc Regulation of microtubule-dependent recycling at the trans-Golgi network by Rab6A and Rab6A'
    Joanne Young
    Cell Biology and Biophysics Programme, European Molecular Biology Laboratory, D 69117 Heidelberg, Germany
    Mol Biol Cell 16:162-77. 2005
    ..Together, this suggests that a recycling pathway operates at the level of the trans-Golgi linking directly to the ER. This pathway would be the preferred route for both toxins and resident Golgi proteins...
  81. pmc The RCP-Rab11 complex regulates endocytic protein sorting
    Andrew A Peden
    Genentech, Inc, South San Francisco, California 94080, USA
    Mol Biol Cell 15:3530-41. 2004
    ..We therefore propose that RCP functions primarily as a Rab11 binding protein that regulates protein sorting in tubular endosomes...
  82. pmc The Rab6-binding kinesin, Rab6-KIFL, is required for cytokinesis
    E Hill
    Beatson Institute for Cancer Research, and University of Glasgow Institute of Biological and Life Sciences, CRC Beatson Laboratories, Garscube Estate, Switchback Road, Bearsden, Glasgow G61 1BD, UK
    EMBO J 19:5711-9. 2000
    ..These data show that endogenous Rab6-KIFL functions in cell division during cleavage furrow formation and cytokinesis, in addition to its previously described role in membrane traffic...
  83. pmc Regulation of endocytic recycling by C. elegans Rab35 and its regulator RME-4, a coated-pit protein
    Miyuki Sato
    Department of Molecular Biology and Biochemistry, Rutgers University, Piscataway, NJ, USA
    EMBO J 27:1183-96. 2008
    ..We propose that RME-4 functions on coated pits and/or vesicles to recruit RAB-35, which in turn functions in the endosome to promote receptor recycling...
  84. ncbi Large scale screening for novel rab effectors reveals unexpected broad Rab binding specificity
    Mitsunori Fukuda
    Laboratory of Membrane Trafficking Mechanisms, Department of Developmental Biology and Neurosciences, Graduate School of Life Sciences, Tohoku University, Aobayama, Aoba ku, Sendai, Miyagi 980 8578, Japan
    Mol Cell Proteomics 7:1031-42. 2008
    ..The findings suggest that most Rab effectors (or Rab-binding proteins) regulate intracellular membrane trafficking through interaction with several Rab isoforms rather than through a single Rab isoform...
  85. pmc Phylogeny and evolution of Rab7 and Rab9 proteins
    Paweł Mackiewicz
    University of Wrocław, Faculty of Biotechnology, Department of Genomics, 63 77 Przybyszewskiego Street, 51 148 Wrocław, Poland
    BMC Evol Biol 9:101. 2009
    ..Rab7 regulates traffic from early to late endosomes and from late endosome to vacuole/lysosome, whereas Rab9 participates in transport from late endosomes to the trans-Golgi network...
  86. ncbi Rab11B small GTPase regulates secretion of cysteine proteases in the enteric protozoan parasite Entamoeba histolytica
    Biswa Nath Mitra
    Department of Parasitology, Gunma University Graduate School of Medicine, 3 39 22 Showa machi, Maebashi, Gunma 371 851, Japan
    Cell Microbiol 9:2112-25. 2007
    ..This is the first report demonstrating that Rab11 plays a central role in the transport and secretion of pathogenic factors...
  87. ncbi The small GTPase Rab6B, a novel Rab6 subfamily member, is cell-type specifically expressed and localised to the Golgi apparatus
    F J Opdam
    Department of Cell Biology, Institute of Cellular Signalling, University of Nijmegen, PO Box 9101, Nijmegen, The Netherlands
    J Cell Sci 113:2725-35. 2000
    ....
  88. ncbi Rab24 is an atypical member of the Rab GTPase family. Deficient GTPase activity, GDP dissociation inhibitor interaction, and prenylation of Rab24 expressed in cultured cells
    R A Erdman
    Weis Center for Research, Penn State College of Medicine, Danville, Pennsylvania 17822, USA
    J Biol Chem 275:3848-56. 2000
    ..These findings indicate that, if Rab24 functions in vesicular transport processes, it may operate through a novel mechanism that does not depend on GTP hydrolysis or GDP dissociation inhibitor-mediated recycling...
  89. pmc Identification and structure of four yeast genes (SLY) that are able to suppress the functional loss of YPT1, a member of the RAS superfamily
    C Dascher
    Department of Molecular Genetics, Max Planck Institute for Biophysical Chemistry, Gottingen, Federal Republic of Germany
    Mol Cell Biol 11:872-85. 1991
    ..SLY12 but not SLY41 is an essential gene. The SLY2 null mutant is cold and heat sensitive. The SLY gene products may comprise elements of the protein transport machinery...
  90. ncbi The small GTPase Rab22 interacts with EEA1 and controls endosomal membrane trafficking
    Maria Kauppi
    Department of Molecular Medicine, National Public Health Institute KTL, Biomedicum, PO Box 104, Fin 00251 Helsinki, Finland
    J Cell Sci 115:899-911. 2002
    ..These results suggest that Rab22a may regulate the dynamic interactions of endosomal compartments and it may be involved in the communication between the biosynthetic and early endocytic pathways...
  91. ncbi The role of ARF and Rab GTPases in membrane transport
    P Chavrier
    Centre d Immunologie INSERM CNRS de Marseille Luminy, Case 906, 13288, Marseille, Cedex 9, France
    Curr Opin Cell Biol 11:466-75. 1999
    ..The past 18 months have seen the identification of proteins that regulate ARF and Rab GDP/GTP cycle, as well as the characterization of their effectors, shedding light on the molecular mechanisms of ARF and Rab function...
  92. pmc Rab-A2 and Rab-A3 GTPases define a trans-golgi endosomal membrane domain in Arabidopsis that contributes substantially to the cell plate
    Cheung Ming Chow
    Department of Plant Sciences, University of Oxford, Oxford OX1 3RB, United Kingdom
    Plant Cell 20:101-23. 2008
    ..Despite the unique features of plant cytokinesis, membrane traffic to the division plane exhibits surprising molecular similarity across eukaryotic kingdoms in its reliance on Ypt3/Rab11/Rab-A GTPases...
  93. ncbi Mutations of the catalytic subunit of RAB3GAP cause Warburg Micro syndrome
    Irene A Aligianis
    Section of Medical and Molecular Genetics, University of Birmingham, Birmingham, B15 2TT, UK
    Nat Genet 37:221-3. 2005
    ..We hypothesize that the underlying pathogenesis of Micro syndrome is a failure of exocytic release of ocular and neurodevelopmental trophic factors...
  94. ncbi Rab11BP/Rabphilin-11, a downstream target of rab11 small G protein implicated in vesicle recycling
    A Mammoto
    Department of Molecular Biology and Biochemistry, Osaka University Medical School, Suita 565 0871, Japan
    J Biol Chem 274:25517-24. 1999
    ..Rabphilin-11 turned out to be a rat counterpart of recently reported bovine Rab11BP. These results indicate that rabphilin-11 is a downstream target of Rab11 which is involved in vesicle recycling...
  95. pmc Docking of yeast vacuoles is catalyzed by the Ras-like GTPase Ypt7p after symmetric priming by Sec18p (NSF)
    A Mayer
    Department of Biochemistry, Dartmouth Medical School, Hanover, New Hampshire 03755 3844, USA
    J Cell Biol 136:307-17. 1997
    ..In addition to Sec17p/Sec18p, docking requires the activity of the Ras-like GTPase Ypt7p. Unlike Sec17p/Sec18p, which must act before docking, Ypt7p is directly involved in the docking process itself...
  96. ncbi Yip3 catalyses the dissociation of endosomal Rab-GDI complexes
    Ulf Sivars
    Department of Biochemistry, Stanford University School of Medicine, Stanford, California 94305 5307, USA
    Nature 425:856-9. 2003
    ..We propose that the conserved Yip proteins serve as GDI-displacement factors for the targeting of Rab GTPases in eukaryotic cells...
  97. ncbi Rab7 is required for the normal progression of the autophagic pathway in mammalian cells
    Maximiliano G Gutierrez
    Laboratorio de Biología Celular y Molecular Instituto de Histología y Embriología, Facultad de Ciencias Medicas, Universidad Nacional de Cuyo CONICET, Mendoza 5500, Argentina
    J Cell Sci 117:2687-97. 2004
    ..Taken together the results indicate that a functional Rab7 is important for the normal progression of autophagy...
  98. ncbi The rat Ruby ( R) locus is Rab38: identical mutations in Fawn-hooded and Tester-Moriyama rats derived from an ancestral Long Evans rat sub-strain
    Naoki Oiso
    Human Medical Genetics Program, University of Colorado Health Sciences Center, 4200 E 9th Ave, B161, Denver, Colorado 80262, USA
    Mamm Genome 15:307-14. 2004
    ..In humans, seven different HPS genes are known. In the mouse, at least 16 loci associated with HPS-like mutant phenotypes are known, seven of which are homologous to the human HPS loci...
  99. pmc Characterization of secretory genes ypt1/yptA and nsf1/nsfA from two filamentous fungi: induction of secretory pathway genes of Trichoderma reesei under secretion stress conditions
    Markku Saloheimo
    VTT Biotechnology, Espoo, Finland Genencor International, Inc, Palo Alto, California, USA
    Appl Environ Microbiol 70:459-67. 2004
    ..These results suggest the possibility that the whole secretory pathway of T. reesei could be induced at the transcriptional level by stress responses caused by protein accumulation in the secretory pathway...
  100. ncbi Identification of a Sec4p GTPase-activating protein (GAP) as a novel member of a Rab GAP family
    L L Du
    Department of Cell Biology, Yale University School of Medicine, New Haven, Connecticut 06520, USA
    J Biol Chem 273:3253-6. 1998
    ..The finding that yeast Rab GAPs share homology with proteins in other species, such as Caenorhabditis elegans and human, suggests the existence of a conserved Rab GAP family...
  101. ncbi Vac1p coordinates Rab and phosphatidylinositol 3-kinase signaling in Vps45p-dependent vesicle docking/fusion at the endosome
    M R Peterson
    Division of Cellular and Molecular Medicine, Howard Hughes Medical Institute, University of California, San Diego, School of Medicine, La Jolla 92093 0668, USA
    Curr Biol 9:159-62. 1999
    ....

Research Grants52

  1. RAB GTP BINDING PROTEINS AND CELL GROWTH
    WILLIAM MALTESE; Fiscal Year: 2005
    ..Ultimately, these insights could reveal new targets for therapeutic intervention in malignant cells. ..
  2. Characterization of a novel nuclear Rab protein
    Ying Huang; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  3. Molecular Mechanisms Controlling Endocytic Recycling
    STEVEN H CAPLAN; Fiscal Year: 2013
    ..Ultimately, these studies will facilitate the development of new strategies to treat the many diseases that arise as a result of aberrant endocytic events. ..
  4. INTRACELLULAR TRANSPORT--THE MANNOSE-PHOSPHATE RECEPTOR
    Suzanne Pfeffer; Fiscal Year: 2008
    ..These studies have important implications for our understanding of growth control and antigen processing, and will provide fundamental information regarding the mechanism of receptor trafficking in mammalian cells. ..
  5. LOCALIZATION OF THE GOLGI COMPLEX
    Suzanne Pfeffer; Fiscal Year: 2001
    ....
  6. Molecular Analysis of the CCC185 Golgin
    Suzanne R Pfeffer; Fiscal Year: 2010
    ..This work has broad implications for our understanding of vesicle docking and fusion events within the secretory and endocytic pathways that are essential for normal human health and disease. ..
  7. Renal Epithelial Sodium Channels and Syntaxins
    Sunil Saxena; Fiscal Year: 2006
    ..Epitope mapping will be used to optimize the specificity of polyclonal antibodies, and anti-sense oligonucleotides will be used to modulate the level of endogenous proteins in the mpkCCDC14 line. ..
  8. Regulation of polarized cell growth by GTPases
    Hay Oak Park; Fiscal Year: 2009
    ..abstract_text> ..
  9. SPATIAL CONTROL OF CELL POLARITY DURING YEAST BUDDING
    Hay Oak Park; Fiscal Year: 2003
    ..This work will also help us to understand the mechanism of genesis of human cancer since mutations in human homologs of these yeast proteins have been found in human tumor. ..
  10. Pseudomonas in Cystic Fibrosis-Regulation of Mucoidy
    Vojo Deretic; Fiscal Year: 2006
    ..These studies are expected to improve our understanding of respiratory pathogenesis in CF, and lead to new treatments of presently incurable lung infections associated with this disease...
  11. Mechanism of M. tuberculosis Phagosome Maturation Arrest
    Vojo Deretic; Fiscal Year: 2008
    ..tuberculosis and provide a foundation for new interventions potentially combating both active disease and latent infection. ..
  12. DEVELOPMENTAL CELL BIOLOGY OF DENDRITIC CELLS
    Ira Mellman; Fiscal Year: 2007
    ..unreadable] [unreadable]..
  13. Autophagy in Tuberculosis
    VOJO P DERETIC; Fiscal Year: 2011
    ..Our studies will provide novel pharmacological and immunological approaches in treatment and prophylaxis of tuberculosis, with strong implications for immunity and infectious diseases in general. ..
  14. Hyperacidification and Pseudomonas infections in CF
    Vojo Deretic; Fiscal Year: 2006
    ..abstract_text> ..
  15. MOLECULAR SORTING DURING INTRACELLULAR TRANSPORT
    Ira Mellman; Fiscal Year: 2006
    ..5) To identify and characterize novel molecules that function during polarized sorting. Cell biological approaches will be applied together with mutant screens and expression analysis in Drosophila. ..
  16. Cell Biology of the Immune Response
    Ira Mellman; Fiscal Year: 2003
    ..4) To explore how the cellular basis of the immune response contributes to the pathogenecity of and potential immunological therapy for cancer and infectious disease. ..
  17. MOLECULAR ANALYSIS OF T CELL RECOGNITION IN EAE
    Elizabeth Ward; Fiscal Year: 2007
    ..affinities, plasticities and cross-reactivities These studies should lead to an improved understanding of the factors that lead to the activation of autoreactive T cells and have general relevance to T cell recognition ..
  18. Embryonic Stem Cell Formation of Definitive Endoderm
    Jeremy Reiter; Fiscal Year: 2005
    ..Ultimately, this approach seeks to provide novel insights how ES cell differentiation into definitive endoderm is regulated, and by extension, how the definitive endoderm arises and develops in vivo. ..
  19. AAV-mediated gene correction in retina
    Jean Bennett; Fiscal Year: 2007
    ..unreadable] [unreadable]..
  20. STAGES OF REGULATED EXOCYTOSIS
    Thomas Martin; Fiscal Year: 2008
    ..__ _ _ i ..
  21. NBCe1 and n1: Crystal Structure Determination
    HARRY GILL; Fiscal Year: 2007
    ..The proposed study will provide key insights on the molecular mechanisms of renal HCO3- transport and will enhance our understanding of processes fundamental to renal disease. [unreadable] [unreadable] [unreadable]..
  22. IMPROVING SURFACE PLASMON RESONANCE TECHNOLOGY
    Elizabeth Ward; Fiscal Year: 2002
    ....
  23. MICROTUBULE/KERATIN INTERACTIONS DURING SPERMATOGENESIS
    ABRAHAM KIERSZENBAUM; Fiscal Year: 2002
    ..Engineering mouse models in which the expression of K9 is null, is important for both reproductive biology and cancer research. ..
  24. GOLGI TARGETING AND RETENTION OF YEAST KEX2 PROTEASE
    Robert Fuller; Fiscal Year: 2009
    ..3. To determine how cargo proteins are recruited to TGN-PVC transport vesicles and how they interact with adaptors or other components of the vesicle coat. ..
  25. Semaphorin-plexin signaling & vertebrate cell movement
    Jeremy Reiter; Fiscal Year: 2003
    ..By illuminating the role of plexin signaling in development, these studies may provide understanding into congenital disease as well as how cell movement is co-opted by pathologic processes such as tumor invasion and tumor angiogenesis. ..
  26. Gene Expression Changes in Early 3D Renal Tubulogenesis
    JOSHUA LIPSCHUTZ; Fiscal Year: 2007
    ..unreadable] [unreadable]..
  27. Animal Model for a Hereditary Macular Degeneration
    Jean Bennett; Fiscal Year: 2004
    ..These studies aim to reveal specific biological pathways relevant to the pathology in this animal model. Any such pathways could potentially be manipulated in the future to slow/prevent the disease process. ..
  28. NFkB, tissue polarity and apoptosis resistance
    Valerie Weaver; Fiscal Year: 2008
    ..Our studies should facilitate the development of rational tumor therapies. ..
  29. Structure-function studies of human FcRn
    Elizabeth Ward; Fiscal Year: 2008
    ..This impacts the successful application of therapeutic and prophylactic IgGs, and also has broader relevance to the factors that regulate humoral immunity. ..
  30. RECEPTOR-MEDIATED ENDOCYTOSIS--MECHANISM AND FUNCTION
    FREDERICK MAXFIELD; Fiscal Year: 2004
    ..These studies will lead to an increasingly complete description of endocytic trafficking pathways. ..
  31. Mechanisms of Rab Protein Activation and Function
    Ruth Collins; Fiscal Year: 2009
    ....
  32. Gene Therapy for Usher Syndrome (USH1C)
    Jean Bennett; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  33. The Exocyst in Synthesis, Cystogenesis and Tubulogenesis
    JOSHUA LIPSCHUTZ; Fiscal Year: 2009
    ..abstract_text> ..
  34. PATHOGENESIS OF RETINAL DEGENERATIONS
    Samuel Jacobson; Fiscal Year: 2001
    ..The results of this research will lead to the formulation of hypotheses about the underlying molecular events in these diseases and may evolve into recommendations for treatment paradigms in these blinding retinal degenerations. ..
  35. INTERACTIONS BETWEEN MICROGLIA AND BETA AMYLOID PLAQUES
    FREDERICK MAXFIELD; Fiscal Year: 2004
    ..We will analyze the mechanisms of degradation of AB amyloid in microglia and also determine whether soluble AB peptides become incorporated into insoluble AB in the endosomes and lysosomes of microglia. ..
  36. CYSTOLIC-FREE CALCIUM AND CELL MOTILITY
    FREDERICK MAXFIELD; Fiscal Year: 2003
    ..Finally, the role of myosin and of oriented recycling will be examined in neutrophils migrating through endothelial cell monolayers and through natural biological matrices, which resemble the physiological sites of neutrophil function. ..
  37. Specificity and Structure: The Kex 2 and Furin Proteases
    Robert Fuller; Fiscal Year: 2005
    ..2. To elucidate the biochemistry and physiology of processing of yeast cell wall proteins by Kex2p and GPI-anchored aspartyl proteases. 3. To achieve Kex2p and furin crystallization to advance determination of their structures. ..
  38. Actin Organization and Polarization
    Liza Pon; Fiscal Year: 2005
    ..The studies proposed are designed to fill that gap. ..
  39. Gene Therapy for Inherited Retinal Degeneration
    Jean Bennett; Fiscal Year: 2007
    ..The ability to rescue photoreceptors in the PDEbeta or abcr -based animal models could ultimately pave the way for genetic therapy as a treatment for blinding retinal diseases. ..
  40. MEMBRANE TRAFFIC IN NORMAL AND TRANSFORMED CELLS
    Peter Novick; Fiscal Year: 2007
    ..A possible cell cycle checkpoint for Golgi partitioning will be evaluated. ..
  41. Antibody engineering: targeting Bacillus anthracis
    Elizabeth Ward; Fiscal Year: 2007
    ..They should also give new insight into understanding the role of antibodies in limiting infection, and this has broader relevance to other pathogens. ..
  42. ANALYSIS OF NEUROFIBROMATOSIS TYPE 1 GENE FUNCTION
    Andre Bernards; Fiscal Year: 2003
    ..These studies will help us understand the normal function of the NF1 gene in vivo and will contribute to our understanding of the diverse manifestations of the disease process. ..
  43. Early-onset retinal degenerations
    Samuel Jacobson; Fiscal Year: 2005
    ....
  44. MMAC1/PTEN TUMOR SUPPRESSOR AND BREAST CANCER
    Gordon Mills; Fiscal Year: 2003
    ..Specific Aim #1: To determine the role of MMAC1 in signal transduction in breast cancer cells. Specific Aim #2: To determine the functional role of MMAC1 in breast cancer pathogenesis. ..
  45. LASER SCANNING CONFOCAL IMAGING SYSTEM
    Liza Pon; Fiscal Year: 2003
    ..I. As an integral component of our Optical Microscopy Facility, the instrument will be accessible to members of the user group and others throughout the University, promoting collaboration and exchange of ideas. ..
  46. CD SIGNALING THROUGH EMT/ITK TYROSINE KINASE
    Gordon Mills; Fiscal Year: 2001
    ..2. To determine the mechanisms regulating the activation of EMT. 3. To determine the mechanisms by which CD28 regulates physiological responses. ..
  47. Host proteins in Vaccinia viral membrane biogenesis
    Victor Hsu; Fiscal Year: 2004
    ..abstract_text> ..
  48. Regulation of exocrine cell secretion by Rab proteins
    Robert Raffaniello; Fiscal Year: 2006
    ..Therefore, an understanding of the basic mechanisms that regulate exocrine gland secretion is essential. [unreadable] [unreadable]..
  49. Modulation of Alzheimer's Amyloidosis by Statins
    Anne M Cataldo; Fiscal Year: 2005
    ....
  50. Advanced Sequence-Based Prediction of Protein Function
    ANDREW NEUWALD; Fiscal Year: 2005
    ..abstract_text> ..
  51. Predicting common protein mechanisms by the light of evolution
    ANDREW NEUWALD; Fiscal Year: 2009
    ..And (v) propose molecular mechanisms based on these analyses. ..