calmodulin

Summary

Summary: A heat-stable, low-molecular-weight activator protein found mainly in the brain and heart. The binding of calcium ions to this protein allows this protein to bind to cyclic nucleotide phosphodiesterases and to adenyl cyclase with subsequent activation. Thereby this protein modulates cyclic AMP and cyclic GMP levels.

Top Publications

  1. ncbi Fluorescent indicators for Ca2+ based on green fluorescent proteins and calmodulin
    A Miyawaki
    Department of Pharmacology, University of California, San Diego, La Jolla 92093 0647, USA
    Nature 388:882-7. 1997
  2. pmc Coping with stresses: roles of calcium- and calcium/calmodulin-regulated gene expression
    Anireddy S N Reddy
    Department of Biology, Program in Molecular Plant Biology, Program in Cell and Molecular Biology, Colorado State University, Fort Collins, Colorado 80523, USA
    Plant Cell 23:2010-32. 2011
  3. ncbi The Ca2+/calmodulin-dependent protein kinase kinases are AMP-activated protein kinase kinases
    Rebecca L Hurley
    Departments of Medicine and Biochemistry, Dartmouth Medical School and the Department of Biological Sciences, Dartmouth College, Hanover, NH 03755, USA
    J Biol Chem 280:29060-6. 2005
  4. ncbi Mechanism of calcium gating in small-conductance calcium-activated potassium channels
    X M Xia
    Vollum Institute, Oregon Health Sciences University, Portland 97201, USA
    Nature 395:503-7. 1998
  5. pmc Calmodulin as a direct detector of Ca2+ signals
    Guido C Faas
    Department of Neurology, University of California Los Angeles, Los Angeles, California, USA
    Nat Neurosci 14:301-4. 2011
  6. ncbi Preassociation of calmodulin with voltage-gated Ca(2+) channels revealed by FRET in single living cells
    M G Erickson
    Department of Biomedical Engineering, Calcium Signals Laboratory, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Neuron 31:973-85. 2001
  7. pmc MicroRNA-1 negatively regulates expression of the hypertrophy-associated calmodulin and Mef2a genes
    Sadakatsu Ikeda
    Department of Cardiology, Children s Hospital Boston, Department of Genetics, Harvard Medical School, Boston, Massachusetts 021151, USA
    Mol Cell Biol 29:2193-204. 2009
  8. ncbi Structures and metal-ion-binding properties of the Ca2+-binding helix-loop-helix EF-hand motifs
    Jessica L Gifford
    Structural Biology Research Group, Department of Biological Sciences, University of Calgary, Calgary, Alberta, Canada T2N 1N4
    Biochem J 405:199-221. 2007
  9. ncbi The complex folding network of single calmodulin molecules
    Johannes Stigler
    Physik Department E22, Technische Universitat Munchen, James Franck Strasse, 85748 Garching, Germany
    Science 334:512-6. 2011
  10. pmc Small conductance Ca2+-activated K+ channels and calmodulin
    James Maylie
    Vollum Institute, Oregon Health and Science University, 3181 SW Sam Jackson Park Road, Portland, OR 97201, USA
    J Physiol 554:255-61. 2004

Detail Information

Publications404 found, 100 shown here

  1. ncbi Fluorescent indicators for Ca2+ based on green fluorescent proteins and calmodulin
    A Miyawaki
    Department of Pharmacology, University of California, San Diego, La Jolla 92093 0647, USA
    Nature 388:882-7. 1997
    ..They consist of tandem fusions of a blue- or cyan-emitting mutant of the green fluorescent protein (GFP), calmodulin, the calmodulin-binding peptide M13, and an enhanced green- or yellow-emitting GFP...
  2. pmc Coping with stresses: roles of calcium- and calcium/calmodulin-regulated gene expression
    Anireddy S N Reddy
    Department of Biology, Program in Molecular Plant Biology, Program in Cell and Molecular Biology, Colorado State University, Fort Collins, Colorado 80523, USA
    Plant Cell 23:2010-32. 2011
    ..In recent years, many Ca(2+) and Ca(2+)/calmodulin (CaM) binding transcription factors (TFs) have been identified in plants...
  3. ncbi The Ca2+/calmodulin-dependent protein kinase kinases are AMP-activated protein kinase kinases
    Rebecca L Hurley
    Departments of Medicine and Biochemistry, Dartmouth Medical School and the Department of Biological Sciences, Dartmouth College, Hanover, NH 03755, USA
    J Biol Chem 280:29060-6. 2005
    ..effects of AMPK on the phosphorylation of acetyl-CoA carboxylase, are largely inhibited by the Ca(2+)/ calmodulin-dependent protein kinase kinase (CaMKK) inhibitor, STO-609...
  4. ncbi Mechanism of calcium gating in small-conductance calcium-activated potassium channels
    X M Xia
    Vollum Institute, Oregon Health Sciences University, Portland 97201, USA
    Nature 395:503-7. 1998
    ..Instead, the functional SK channels are heteromeric complexes with calmodulin, which is constitutively associated with the alpha-subunits in a calcium-independent manner...
  5. pmc Calmodulin as a direct detector of Ca2+ signals
    Guido C Faas
    Department of Neurology, University of California Los Angeles, Los Angeles, California, USA
    Nat Neurosci 14:301-4. 2011
    ..it is this buffered level of Ca(2+) that triggers downstream biochemical processes, notably the activation of calmodulin (CaM) and the resulting activation of CaM-dependent enzymes...
  6. ncbi Preassociation of calmodulin with voltage-gated Ca(2+) channels revealed by FRET in single living cells
    M G Erickson
    Department of Biomedical Engineering, Calcium Signals Laboratory, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Neuron 31:973-85. 2001
    ..is the regulation of L-type and P/Q-type channels by intracellular Ca(2+), acting via unconventional channel-calmodulin (CaM) interactions...
  7. pmc MicroRNA-1 negatively regulates expression of the hypertrophy-associated calmodulin and Mef2a genes
    Sadakatsu Ikeda
    Department of Cardiology, Children s Hospital Boston, Department of Genetics, Harvard Medical School, Boston, Massachusetts 021151, USA
    Mol Cell Biol 29:2193-204. 2009
    Calcium signaling is a central regulator of cardiomyocyte growth and function. Calmodulin is a critical mediator of calcium signals...
  8. ncbi Structures and metal-ion-binding properties of the Ca2+-binding helix-loop-helix EF-hand motifs
    Jessica L Gifford
    Structural Biology Research Group, Department of Biological Sciences, University of Calgary, Calgary, Alberta, Canada T2N 1N4
    Biochem J 405:199-221. 2007
    ..A structural approach is used in this review to examine the diversity of family members, and a biophysical perspective provides insight into the ability of the EF-hand motif to bind Ca2+ with a wide range of affinities...
  9. ncbi The complex folding network of single calmodulin molecules
    Johannes Stigler
    Physik Department E22, Technische Universitat Munchen, James Franck Strasse, 85748 Garching, Germany
    Science 334:512-6. 2011
    ..We have used single-molecule force spectroscopy to study the folding transitions of single calmodulin molecules...
  10. pmc Small conductance Ca2+-activated K+ channels and calmodulin
    James Maylie
    Vollum Institute, Oregon Health and Science University, 3181 SW Sam Jackson Park Road, Portland, OR 97201, USA
    J Physiol 554:255-61. 2004
    ..that Ca(2+) gating in SK channels is due to heteromeric assembly of the SK alpha pore-forming subunits with calmodulin (CaM). Ca(2+) binding to the N-terminal E-F hands of CaM is responsible for SK channel gating...
  11. ncbi Ca2+ indicators based on computationally redesigned calmodulin-peptide pairs
    Amy E Palmer
    Department of Pharmacology and Howard Hughes Medical Institute, University of California, San Diego, 9500 Gilman Drive, La Jolla, California 92093, USA
    Chem Biol 13:521-30. 2006
    The binding interface of calmodulin and a calmodulin binding peptide were reengineered by computationally designing complementary bumps and holes...
  12. pmc Insights into voltage-gated calcium channel regulation from the structure of the CaV1.2 IQ domain-Ca2+/calmodulin complex
    Filip Van Petegem
    Cardiovascular Research Institute, Department of Biochemistry and Biophysics, California Institute for Quantitative Biomedical Research, University of California, San Francisco, 1700 4th St, Box 2532, San Francisco, California 94143 2532, USA
    Nat Struct Mol Biol 12:1108-15. 2005
    ..Ca(V)s) drive two self-regulatory calcium-dependent feedback processes that require interaction between Ca(2+)/calmodulin (Ca(2+)/CaM) and a Ca(V) channel consensus isoleucine-glutamine (IQ) motif: calcium-dependent inactivation (CDI) ..
  13. pmc Mechanism of local and global Ca2+ sensing by calmodulin in complex with a Ca2+ channel
    Michael R Tadross
    Calcium Signals Laboratory, Departments of Biomedical Engineering and Neuroscience, The Johns Hopkins University School of Medicine, Ross Building, Room 713, 720 Rutland Avenue, Baltimore, MD 21205, USA
    Cell 133:1228-40. 2008
    b>Calmodulin (CaM) in complex with Ca(2+) channels constitutes a prototype for Ca(2+) sensors that are intimately colocalized with Ca(2+) sources...
  14. ncbi Calmodulin: a prototypical calcium sensor
    D Chin
    Dept of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, NC 27710, USA
    Trends Cell Biol 10:322-8. 2000
    b>Calmodulin is the best studied and prototypical example of the E-F-hand family of Ca2+-sensing proteins. Changes in intracellular Ca2+ concentration regulate calmodulin in three distinct ways...
  15. pmc Crystal structure of the CaV2 IQ domain in complex with Ca2+/calmodulin: high-resolution mechanistic implications for channel regulation by Ca2+
    Masayuki X Mori
    Calcium Signals Laboratory, Department of Biomedical Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Structure 16:607-20. 2008
    b>Calmodulin (CaM) regulation of Ca(2+) channels is central to Ca(2+) signaling...
  16. pmc Dynamic modulation of ANO1/TMEM16A HCO3(-) permeability by Ca2+/calmodulin
    Jinsei Jung
    Department of Pharmacology, Brain Korea 21 Project for Medical Sciences, Yonsei University College of Medicine, Seoul 120 752, Korea
    Proc Natl Acad Sci U S A 110:360-5. 2013
    ..Here, we report that the anion selectivity of ANO1 is dynamically regulated by the Ca(2+)/calmodulin complex...
  17. pmc Crystal structure of the ternary complex of a NaV C-terminal domain, a fibroblast growth factor homologous factor, and calmodulin
    Chaojian Wang
    Division of Cardiology, Department of Medicine, Duke University Medical Center, 2 Genome Ct, Durham, NC 27710, USA
    Structure 20:1167-76. 2012
    ..interactions with many auxiliary proteins, including fibroblast growth factor homologous factors (FHFs) and calmodulin (CaM)...
  18. pmc Crystal structure of dimeric cardiac L-type calcium channel regulatory domains bridged by Ca2+* calmodulins
    Jennifer L Fallon
    Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX 77030, USA
    Proc Natl Acad Sci U S A 106:5135-40. 2009
    ..Ca(V)) open in response to changes in membrane potential, but their activity is modulated by Ca(2+) binding to calmodulin (CaM)...
  19. ncbi Calmodulin-dependent activation of MAP kinase for ROS homeostasis in Arabidopsis
    Fuminori Takahashi
    Gene Discovery Research Group, RIKEN Plant Science Center, 3 1 1 Koyadai, Tsukuba, Ibaraki 305 0074, Japan
    Mol Cell 41:649-60. 2011
    ..These findings suggest that two major activation modes in eukaryotes, Ca²+/CaMs and the MAP kinase phosphorylation cascade, converge at MPK8 to monitor or maintain an essential part of ROS homeostasis...
  20. ncbi Autophosphorylation at Thr286 of the alpha calcium-calmodulin kinase II in LTP and learning
    K P Giese
    Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA
    Science 279:870-3. 1998
    The calcium-calmodulin-dependent kinase II (CaMKII) is required for hippocampal long-term potentiation (LTP) and spatial learning...
  21. pmc Excitation-transcription coupling via calcium/calmodulin-dependent protein kinase/ERK1/2 signaling mediates the coordinate induction of VGLUT2 and Narp triggered by a prolonged increase in glutamatergic synaptic activity
    Sukhjeevan Doyle
    Neuroscience Center of Excellence, Louisiana State University Health Sciences Center, New Orleans, Louisiana 70112, USA
    J Biol Chem 285:14366-76. 2010
    ..glutamate receptors and L-type voltage-gated calcium channels, and is dependent on downstream signaling via calmodulin, calcium/calmodulin-dependent protein kinase (CaMK) and extracellular signal-regulated kinase 1/2 (ERK1/2)...
  22. ncbi Signaling to the nucleus by an L-type calcium channel-calmodulin complex through the MAP kinase pathway
    R E Dolmetsch
    Division of Neuroscience, Children's Hospital and Department of Neurobiology, Harvard Medical School, Enders Pediatric Research Laboratories, Room 260, 300 Longwood Avenue, Boston, MA 02115, USA
    Science 294:333-9. 2001
    ..We found that an isoleucine-glutamine ("IQ") motif in the carboxyl terminus of the LTC that binds Ca2+-calmodulin (CaM) is critical for conveying the Ca2+ signal to the nucleus...
  23. ncbi Solution structure of Ca(2+)-calmodulin reveals flexible hand-like properties of its domains
    J J Chou
    Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892 0520, USA
    Nat Struct Biol 8:990-7. 2001
    The solution structure of Ca(2+)-ligated calmodulin is determined from residual dipolar couplings measured in a liquid crystalline medium and from a large number of heteronuclear J couplings for defining side chains...
  24. pmc Structure of the CaMKIIdelta/calmodulin complex reveals the molecular mechanism of CaMKII kinase activation
    Peter Rellos
    University of Oxford, Nuffield Department of Clinical Medicine, Structural Genomics Consortium, Oxford, United Kingdom
    PLoS Biol 8:e1000426. 2010
    ..LTP triggers high-frequency calcium pulses that result in the activation of Calcium/Calmodulin (CaM)-dependent kinase II (CaMKII)...
  25. ncbi A calcium sensor in the sodium channel modulates cardiac excitability
    Hanno L Tan
    Department of Anesthesiology, Vanderbilt University School of Medicine, Nashville 37232, Tennessee, USA
    Nature 415:442-7. 2002
    ..Here we show that calmodulin (CaM), a ubiquitous Ca2+-sensing protein, binds to the carboxy-terminal 'IQ' domain of the human cardiac Na ..
  26. pmc Combined proteomic and cytological analysis of Ca2+-calmodulin regulation in Picea meyeri pollen tube growth
    Tong Chen
    Key Laboratory of Photosynthesis and Molecular Environmental Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China
    Plant Physiol 149:1111-26. 2009
    Ca2+-calmodulin (Ca2+-CaM) is a critical molecule that mediates cellular functions by interacting with various metabolic and signaling pathways...
  27. ncbi Ca(2+)-activated K+ channels: molecular determinants and function of the SK family
    Martin Stocker
    Wellcome Laboratory for Molecular Pharmacology, Department of Pharmacology, University College London, Gower Street, London WC1E 6BT, UK
    Nat Rev Neurosci 5:758-70. 2004
    ....
  28. pmc Vacuolar Na+/H+ antiporter cation selectivity is regulated by calmodulin from within the vacuole in a Ca2+- and pH-dependent manner
    Toshio Yamaguchi
    Department of Plant Sciences, University of California, Davis, CA 95616, USA
    Proc Natl Acad Sci U S A 102:16107-12. 2005
    ..Here, we have identified A. thaliana calmodulin-like protein 15 (AtCaM15), which interacts with the AtNHX1 C terminus...
  29. ncbi Sustained LFA-1 cluster formation in the immune synapse requires the combined activities of L-plastin and calmodulin
    Guido H Wabnitz
    Institute for Immunology, Ruprecht Karls University, Heidelberg, Germany
    Eur J Immunol 40:2437-49. 2010
    ..in the IS is a consequence of the combined activities of the actin-bundling protein L-plastin (LPL) and calmodulin. Thus, upon antigen-recognition of T cells, LPL accumulated predominantly in the peripheral SMAC...
  30. ncbi Structure of the autoinhibited kinase domain of CaMKII and SAXS analysis of the holoenzyme
    Oren S Rosenberg
    Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA
    Cell 123:849-60. 2005
    Ca2+/calmodulin-dependent protein kinase-II (CaMKII) is unique among protein kinases for its dodecameric assembly and its complex response to Ca2+. The crystal structure of the autoinhibited kinase domain of CaMKII, determined at 1...
  31. pmc Specific nuclear localizing sequence directs two myosin isoforms to the cell nucleus in calmodulin-sensitive manner
    Rastislav Dzijak
    Department of Biology of the Cell Nucleus, Institute of Molecular Genetics of the ASCR, v v i, Prague, Czech Republic
    PLoS ONE 7:e30529. 2012
    ..NM1 differs from the "cytoplasmic" myosin 1c only by additional 16 amino acids at the N-terminus of the molecule. This amino acid stretch was therefore suggested to direct NM1 into the nucleus...
  32. ncbi Fast methionine-based solution structure determination of calcium-calmodulin complexes
    Jessica L Gifford
    Department of Biological Sciences, University of Calgary, Calgary, AB, Canada
    J Biomol NMR 50:71-81. 2011
    Here we present a novel NMR method for the structure determination of calcium-calmodulin (Ca(2+)-CaM)-peptide complexes from a limited set of experimental restraints...
  33. ncbi Phosphorylation of Thr(495) regulates Ca(2+)/calmodulin-dependent endothelial nitric oxide synthase activity
    I Fleming
    Institut für Kardiovaskuläre Physiologie, Klinikum der J W G Universität, Frankfurt, Germany
    Circ Res 88:E68-75. 2001
    ..high-output) activation of eNOS is associated with changes in the phosphorylation of Thr(495) in the calmodulin (CaM)-binding domain...
  34. ncbi Ca(2+) and calmodulin initiate all forms of endocytosis during depolarization at a nerve terminal
    Xin Sheng Wu
    National Institute of Neurological Disorders and Stroke, Bethesda, Maryland, USA
    Nat Neurosci 12:1003-10. 2009
    ..Pharmacological experiments suggested that the calcium sensor mediating these forms of endocytosis is calmodulin. In addition to its role in recycling vesicles, calcium/calmodulin-initiated endocytosis facilitated vesicle ..
  35. ncbi Calmodulin signaling via the IQ motif
    Martin Bähler
    Institut für Allegemeine Zoologie und Genetik, Westfalische Wilhelms Universitat, Munster, Germany
    FEBS Lett 513:107-13. 2002
    ..An examination of IQ motif-containing proteins that are known to bind calmodulin (CaM) indicates a wide diversity of biological functions that parallel the Ca2+-dependent targets...
  36. ncbi Interaction with the NMDA receptor locks CaMKII in an active conformation
    K U Bayer
    Department of Neurobiology, Stanford University School of Medicine, California 94305 5125, USA
    Nature 411:801-5. 2001
    Calcium- and calmodulin-dependent protein kinase II (CaMKII) and glutamate receptors are integrally involved in forms of synaptic plasticity that may underlie learning and memory...
  37. ncbi Reconstitution of an endothelial nitric-oxide synthase (eNOS), hsp90, and caveolin-1 complex in vitro. Evidence that hsp90 facilitates calmodulin stimulated displacement of eNOS from caveolin-1
    J P Gratton
    Department of Pharmacology and Program of Molecular Cardiobiology, Boyer Center for Molecular Medicine, Yale University School of Medicine, New Haven, Connecticut 06536, USA
    J Biol Chem 275:22268-72. 2000
    ..with exogenous glutathione S-transferase-linked caveolin-1 (GST-Cav), and the addition of calcium-activated calmodulin (CaM) to the GST-Cav complex partially inhibited the association of eNOS and hsp90...
  38. ncbi Calcium/calmodulin-mediated signal network in plants
    Tianbao Yang
    Center for Integrated Biotechnology and Department of Horticulture, Washington State University, Pullman, WA 99164 6414, USA
    Trends Plant Sci 8:505-12. 2003
    ..b>Calmodulin, the predominant calcium receptor, is one of the best-characterized calcium sensors in eukaryotes...
  39. ncbi NuMA-related LIN-5, ASPM-1, calmodulin and dynein promote meiotic spindle rotation independently of cortical LIN-5/GPR/Galpha
    Monique van der Voet
    Developmental Biology, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands
    Nat Cell Biol 11:269-77. 2009
    ..ASPM-1, together with calmodulin (CMD-1), promotes meiotic spindle organization and the accumulation of LIN-5 at meiotic and mitotic spindle ..
  40. ncbi Modulation of calmodulin plasticity in molecular recognition on the basis of x-ray structures
    W E Meador
    Howard Hughes Medical Institute, Baylor College of Medicine, Houston, TX 77030
    Science 262:1718-21. 1993
    b>Calmodulin is the primary calcium-dependent signal transducer and regulator of a wide variety of essential cellular functions...
  41. ncbi Plasma membrane phosphoinositide organization by protein electrostatics
    Stuart McLaughlin
    Department of Physiology and Biophysics, Health Sciences Center, Stony Brook University, Stony Brook, New York 11794, USA
    Nature 438:605-11. 2005
    ..Recent studies suggest that membrane proteins concentrate PIP2 and, in response to local increases in intracellular calcium concentration, release it to interact with other biologically important molecules...
  42. ncbi Calmodulin's flexibility allows for promiscuity in its interactions with target proteins and peptides
    Aaron P Yamniuk
    Structural Biology Research Group, Department of Biological Sciences, University of Calgary, 2500 University Drive NW, Calgary, AB, Canada
    Mol Biotechnol 27:33-57. 2004
    The small bilobal calcium regulatory protein calmodulin (CaM) activates numerous target enzymes in response to transient changes in intracellular calcium concentrations...
  43. pmc Differential binding of calmodulin-related proteins to their targets revealed through high-density Arabidopsis protein microarrays
    Sorina C Popescu
    Department of Molecular, Cellular, and Developmental Biology, Yale University, 219 Prospect Street, New Haven, CT 06520 8103, USA
    Proc Natl Acad Sci U S A 104:4730-5. 2007
    ..Our results suggest that calcium functions through distinct CaM/CML proteins to regulate a wide range of targets and cellular activities...
  44. ncbi Molecular basis of calmodulin tethering and Ca2+-dependent inactivation of L-type Ca2+ channels
    G S Pitt
    Department of Molecular and Cellular Physiology, Stanford University Medical School, Stanford, California 94305, USA
    J Biol Chem 276:30794-802. 2001
    ..Ca(2+)-insensitive forms of calmodulin (CaM) act as dominant negatives to prevent CDI, suggesting that CaM acts as a resident Ca(2+) sensor...
  45. ncbi Calmodulin and calmodulin-like proteins in plant calcium signaling
    Alexandre Perochon
    UMR 5546 CNRS Université Paul Sabatier Toulouse III, Pole de Biotechnologie Vegetale, Auzeville, Castanet Tolosan Cedex, France
    Biochimie 93:2048-53. 2011
    b>Calmodulin (CaM) is a primary calcium sensor in all eukaryotes. It binds calcium and regulates the activity of a wide range of effector proteins in response to calcium signals...
  46. pmc Nitric oxide-activated calcium/calmodulin-dependent protein kinase regulates the abscisic acid-induced antioxidant defence in maize
    Fangfang Ma
    College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, People s Republic of China
    J Exp Bot 63:4835-47. 2012
    Nitric oxide (NO), hydrogen peroxide (H2O2), and calcium (Ca2+)/calmodulin (CaM) are all required for abscisic acid (ABA)-induced antioxidant defence...
  47. pmc Calcium-dependent folding of single calmodulin molecules
    Johannes Stigler
    Physik Department E22, Technische Universitat Munchen, James Franck Strasse, 85748 Garching, Germany
    Proc Natl Acad Sci U S A 109:17814-9. 2012
    b>Calmodulin is the primary calcium binding protein in living cells. Its function and structure depend strongly on calcium concentration...
  48. pmc Calmodulin activation of Aurora-A kinase (AURKA) is required during ciliary disassembly and in mitosis
    Olga V Plotnikova
    Developmental Therapeutics Program, Fox Chase Cancer Center, Philadelphia, PA 19111, USA
    Mol Biol Cell 23:2658-70. 2012
    ..show here that AURKA activation at the basal body in ciliary disassembly requires interactions with Ca(2+) and calmodulin (CaM) and that Ca(2+)/CaM are important mediators of the ciliary disassembly process...
  49. ncbi Unified mechanisms of Ca2+ regulation across the Ca2+ channel family
    Haoya Liang
    Ca2 Signals Laboratory, Departments of Biomedical Engineering and Neuroscience, The Johns Hopkins University School of Medicine, 720 Rutland Avenue, Baltimore, MD 21205, USA
    Neuron 39:951-60. 2003
    ....
  50. ncbi Ca(2+)/calmodulin regulates salicylic-acid-mediated plant immunity
    Liqun Du
    Center for Integrated Biotechnology and Department of Horticulture, Washington State University, Pullman, Washington 99164 6414, USA
    Nature 457:1154-8. 2009
    ..Here we report a mechanism connecting Ca(2+) signal to salicylic-acid-mediated immune response through calmodulin, AtSR1 (also known as CAMTA3), a Ca(2+)/calmodulin-binding transcription factor, and EDS1, an established ..
  51. pmc Structural basis for calmodulin as a dynamic calcium sensor
    Miao Zhang
    Department of Molecular Physiology and Biophysics, Jefferson Medical College, Philadelphia, PA 19107, USA
    Structure 20:911-23. 2012
    b>Calmodulin is a prototypical and versatile Ca(2+) sensor with EF hands as its high-affinity Ca(2+) binding domains. Calmodulin is present in all eukaryotic cells, mediating Ca(2+)-dependent signaling...
  52. ncbi A new polymorphism in human calmodulin III gene promoter is a potential modifier gene for familial hypertrophic cardiomyopathy
    Felix W Friedrich
    Institute of Experimental and Clinical Pharmacology and Toxicology, University Medical Center Hamburg Eppendorf, Hamburg, Germany
    Eur Heart J 30:1648-55. 2009
    ..Incomplete penetrance suggests the existence of modifier genes. Calmodulin (CaM) could be of importance given the key role of Ca(2+) for cardiac contractile function and growth...
  53. pmc Modulation of skeletal and cardiac voltage-gated sodium channels by calmodulin
    Katharine A Young
    Campus Box 8315, Dept of Cell Devel Biology and the Neuroscience Program, UCHSC, PO Box 6511, Aurora, CO 80045, USA
    J Physiol 565:349-70. 2005
    b>Calmodulin (CaM) has been shown to modulate different ion channels, including voltage-gated sodium channels (NaChs). Using the yeast two-hybrid assay, we found an interaction between CaM and the C-terminal domains of adult skeletal (NaV1...
  54. pmc STIM1 and calmodulin interact with Orai1 to induce Ca2+-dependent inactivation of CRAC channels
    Franklin M Mullins
    Department of Pathology, Stanford University School of Medicine, Stanford, CA 94305, USA
    Proc Natl Acad Sci U S A 106:15495-500. 2009
    ..Based on bioinformatics predictions of a calmodulin (CaM) binding site on Orai1, we also investigated a role for CaM in CDI...
  55. ncbi Three-dimensional structure of calmodulin
    Y S Babu
    Nature 315:37-40. 1985
    The three-dimensional structure of calmodulin has been determined crystallographically at 3.0 A resolution. The molecule consists of two globular lobes connected by a long exposed alpha-helix...
  56. pmc Structural and energetic determinants of apo calmodulin binding to the IQ motif of the Na(V)1.2 voltage-dependent sodium channel
    Michael D Feldkamp
    Department of Biochemistry, Roy J and Lucille A Carver College of Medicine, University of Iowa, Iowa City, IA 52242 1109, USA
    Structure 19:733-47. 2011
    ..2), essential for generation and propagation of action potentials, is regulated by calmodulin (CaM) binding to the IQ motif in its α subunit. A peptide (Na(v)1...
  57. ncbi Calmodulin methyltransferase is an evolutionarily conserved enzyme that trimethylates Lys-115 in calmodulin
    Roberta Magnani
    Department of Horticulture, Plant Physiology Biochemistry Molecular Biology Program, University of Kentucky, Lexington, Kentucky 40546, USA
    Nat Commun 1:43. 2010
    b>Calmodulin (CaM) is a key mediator of calcium-dependent signalling and is subject to regulatory post-translational modifications, including trimethylation of Lys-115...
  58. ncbi Breaking the code: Ca2+ sensors in plant signalling
    Thomas A DeFalco
    Department of Biology, Queen s University, Kingston, Ontario, Canada K7L 3N6
    Biochem J 425:27-40. 2010
    ..In the present review, we discuss recent advances in research on CaMs and CMLs, CDPKs and CBLs, and we attempt to integrate the current knowledge on the different sensor classes into common physiological themes...
  59. pmc An IQ domain mediates the interaction with calmodulin in a plant cyclic nucleotide-gated channel
    Cornelia Fischer
    Molekulare Pflanzenphysiologie and Erlangen Center of Plant Science, Department Biology, Friedrich Alexander Universitat Erlangen Nurnberg, Staudtstraße 5, D 91058 Erlangen, Germany
    Plant Cell Physiol 54:573-84. 2013
    Cyclic nucleotide-gated channels (CNGCs) form non-selective cation entry pathways regulated by calmodulin (CaM), a universal Ca(2+) sensor in eukaryotes...
  60. ncbi MARCKS is an actin filament crosslinking protein regulated by protein kinase C and calcium-calmodulin
    J H Hartwig
    Department of Medicine, Brigham and Women s Hospital, Boston, Massachusetts
    Nature 356:618-22. 1992
    ..The myristoylated, alanine-rich C kinase substrate (MARCKS) binds calmodulin and is a predominant, specific substrate of PKC which is phosphorylated during macrophage and neutrophil ..
  61. pmc Structural basis for the differential effects of CaBP1 and calmodulin on Ca(V)1.2 calcium-dependent inactivation
    Felix Findeisen
    Cardiovascular Research Institute, University of California, San Francisco, CA 94158 2330, USA
    Structure 18:1617-31. 2010
    Calcium-binding protein 1 (CaBP1), a calmodulin (CaM) homolog, endows certain voltage-gated calcium channels (Ca(V)s) with unusual properties. CaBP1 inhibits Ca(V)1...
  62. ncbi Ionic interactions are essential for TRPV1 C-terminus binding to calmodulin
    Lenka Grycova
    Institute of Physiology, Academy of Sciences of the Czech Republic, Videnska 1083, 14220 Prague, Czech Republic
    Biochem Biophys Res Commun 375:680-3. 2008
    b>Calmodulin (CaM) is known to play an important role in the regulation of TRP channels activity. Although it has been reported that CaM binds to the C-terminus of TRPV1 (TRPV1-CT), no classic CaM-binding motif was found in this region...
  63. ncbi Protein-peptide interaction studies demonstrate the versatility of calmodulin target protein binding
    Hiroaki Ishida
    Structural Biology Research Group, Department of Biological Sciences, University of Calgary, Calgary, Alberta, T2N 1N4, Canada
    Protein Pept Lett 13:455-65. 2006
    b>Calmodulin (CaM) is a prototypical Ca2+-sensor protein that can control many important biological functions by binding to hundreds of target proteins...
  64. ncbi Calmodulin interacts with angiotensin-converting enzyme-2 (ACE2) and inhibits shedding of its ectodomain
    Daniel W Lambert
    Institute of Molecular and Cellular Biology, Faculty of Biological Sciences, University of Leeds, Leeds, UK
    FEBS Lett 582:385-90. 2008
    ..In this study, we demonstrate that inhibitors of calmodulin also stimulate shedding of the ACE2 ectodomain, a process at least partially mediated by a metalloproteinase...
  65. ncbi Calcium/calmodulin-dependent protein kinase II contributes to activity-dependent filopodia growth and spine formation
    Pascal Jourdain
    Neuropharmacology, University Medical Center, University of Geneva, 1211 Geneva 4, Switzerland
    J Neurosci 23:10645-9. 2003
    ..Here, we investigated whether calcium/calmodulin-dependent protein kinase II (CaMKII), an enzyme implicated in the control of synaptic efficacy, also ..
  66. pmc IQGAP1, a Rac- and Cdc42-binding protein, directly binds and cross-links microfilaments
    A M Bashour
    Department of Cell Biology and Neuroscience, University of Texas Southwestern Medical Center, Dallas, Texas 75235, USA
    J Cell Biol 137:1555-66. 1997
    ..purified from bovine adrenal comprises two approximately 190-kD subunits per molecule plus substoichiometric calmodulin. Purified IQGAP1 bound directly to F-actin and cross-linked the actin filaments into irregular, interconnected ..
  67. pmc Interactions between calmodulin, adenosine A2A, and dopamine D2 receptors
    Gemma Navarro
    Institut d Investigacions Biomediques August Pi i Sunyer, Centro de Investigacion Biomedica en Red sobre Enfermedades Neurodegenerativas, and Department of Biochemistry and Molecular Biology, Faculty of Biology, University of Barcelona, 08028 Barcelona, Spain
    J Biol Chem 284:28058-68. 2009
    The Ca(2+)-binding protein calmodulin (CaM) has been shown to bind directly to cytoplasmic domains of some G protein-coupled receptors, including the dopamine D(2) receptor...
  68. ncbi A relationship between protein kinase C phosphorylation and calmodulin binding to the metabotropic glutamate receptor subtype 7
    Y Nakajima
    Department of Cell Physiology, National Institute for Physiological Sciences, Okazaki 444 8585, Japan
    J Biol Chem 274:27573-7. 1999
    ..Here, we report that calmodulin (CaM) directly binds to the carboxyl terminus of mGluR7 in a Ca(2+)-dependent manner...
  69. ncbi Calmodulin and Munc13 form a Ca2+ sensor/effector complex that controls short-term synaptic plasticity
    Harald J Junge
    Department of Molecular Neurobiology, Max Planck Institute for Experimental Medicine, Hermann Rein Strasse 3, D 37077 Gottingen, Germany
    Cell 118:389-401. 2004
    ..We now identify a conserved calmodulin binding site in UNC-13/Munc13s, which are essential regulators of synaptic vesicle priming and synaptic efficacy...
  70. pmc Solution NMR structure of Apo-calmodulin in complex with the IQ motif of human cardiac sodium channel NaV1.5
    Benjamin Chagot
    Department of Biochemistry, Vanderbilt University, Nashville, TN 37232, USA
    J Mol Biol 406:106-19. 2011
    ..5 is regulated in part by intracellular calcium signals. The ubiquitous calcium sensor protein calmodulin (CaM) is an important part of the complex calcium-sensing apparatus in Na(V)1.5...
  71. ncbi Pcp1p, an Spc110p-related calmodulin target at the centrosome of the fission yeast Schizosaccharomyces pombe
    Mark R Flory
    Molecular and Cellular Biology Program, University of Washington, Seattle, Washington 98195, USA
    Cell Growth Differ 13:47-58. 2002
    In the budding yeast Saccharomyces cerevisiae, the calmodulin-binding protein Spc110p/Nuf1p facilitates mitotic spindle formation from the fungal centrosome or spindle pole body (SPB)...
  72. pmc Molecular phylogeny of Sporothrix schenckii
    Rita Marimon
    Departament de Ciencies Mediques Basiques, Unitat de Microbiologia, Facultat de Medicina i Ciencies de la Salut, Universitat Rovira i Virgili, Carrer Sant Llorenc 21, 43201 Reus, Tarragona, Spain
    J Clin Microbiol 44:3251-6. 2006
    ..study was to determine, by sequence analysis of three protein coding loci (chitin synthase, beta-tubulin, and calmodulin), whether this variability is due to species divergence or intraspecific diversity in S. schenckii...
  73. ncbi Handling calcium signaling: Arabidopsis CaMs and CMLs
    Elizabeth McCormack
    Biochemistry and Cell Biology, Rice University, Houston, TX 77005 1892, USA
    Trends Plant Sci 10:383-9. 2005
    The Arabidopsis genome harbors seven calmodulin (CAM) and 50 CAM-like (CML) genes that encode potential calcium sensors. The CAMs encode only four protein isoforms. Selective pressure to maintain multiple CAMs indicates nonredundancy...
  74. pmc Crystal structure of apo-calmodulin bound to the first two IQ motifs of myosin V reveals essential recognition features
    Anne Houdusse
    Motilité Structurale, Institut Curie, Centre National de la Recherche Scientifique, Unite Mixte de Recherche 144, 26 Rue d Ulm, 75248, Paris Cedex 05, France
    Proc Natl Acad Sci U S A 103:19326-31. 2006
    A 2.5-A resolution structure of calcium-free calmodulin (CaM) bound to the first two IQ motifs of the murine myosin V heavy chain reveals an unusual CaM conformation...
  75. ncbi Multiple roles of calmodulin and other Ca(2+)-binding proteins in the functional regulation of TRP channels
    Michael Xi Zhu
    Department of Neuroscience and the Center for Molecular Neurobiology, The Ohio State University, 168 Rightmire Hall, 1060 Carmack Road, Columbus, OH 43210, USA
    Pflugers Arch 451:105-15. 2005
    ..Among them, Ca(2+)-mediated feedback channel modulations are often mediated by calmodulin (CaM) and other Ca(2+)-binding proteins...
  76. ncbi Structure of calmodulin refined at 2.2 A resolution
    Y S Babu
    Department of Pathology, University of Alabama, Birmingham 35294
    J Mol Biol 204:191-204. 1988
    The crystal structure of mammalian calmodulin has been refined at 2.2 A (1 A = 0.1 nm) resolution using a restrained least-squares method. The final crystallographic R-factor, based on 6685 reflections in the range 2...
  77. ncbi Post-translational control of endothelial nitric oxide synthase: why isn't calcium/calmodulin enough?
    D Fulton
    Department of Pharmacology and Molecular Cardiobiology Program, Boyer Center for Molecular Medicine, Yale University School of Medicine, New Haven, Connecticut 06536, USA
    J Pharmacol Exp Ther 299:818-24. 2001
    ..Indeed calcium-activated calmodulin is important for regulation of NOS activity...
  78. pmc Calmodulins and calcineurin B-like proteins: calcium sensors for specific signal response coupling in plants
    Sheng Luan
    Department of Plant and Microbial Biology, University of California, Berkeley, CA 94720, USA
    Plant Cell 14:S389-400. 2002
  79. ncbi IL-10 regulation by HIV-Tat in primary human monocytic cells: involvement of calmodulin/calmodulin-dependent protein kinase-activated p38 MAPK and Sp-1 and CREB-1 transcription factors
    Katrina Gee
    Department of Pathology and Laboratory Medicine, Division of Virology and Molecular Immunology, Children s Hospital of Eastern Ontario, 401 Smyth Road, Ottawa, Ontario, Canada
    J Immunol 178:798-807. 2007
    ..The experiments designed to further understand the molecules involved in the calcium signaling suggested that calmodulin and calmodulin-dependent protein kinase-II (CaMK-II)-activated p38 MAPK played a role in extracellular Tat-..
  80. pmc Engineered calmodulins reveal the unexpected eminence of Ca2+ channel inactivation in controlling heart excitation
    Badr A Alseikhan
    Calcium Signals Laboratory, Program in Molecular and Cellular Systems Physiology, Departments of Biomedical Engineering and Neuroscience, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Proc Natl Acad Sci U S A 99:17185-90. 2002
    ..This striking result recasts the basic paradigm for action-potential control and illustrates the promise of virally delivered engineered CaM to investigate the biology of numerous other CaM-signaling pathways...
  81. pmc Rapid transcriptome changes induced by cytosolic Ca2+ transients reveal ABRE-related sequences as Ca2+-responsive cis elements in Arabidopsis
    Boaz Kaplan
    Department of Plant Sciences, Weizman Institute of Science, Rehovot 76100, Israel
    Plant Cell 18:2733-48. 2006
    ..A cytosolic calcium transient induced by calmodulin antagonists and blocked by lanthanides was characterized using aequorin-based luminometry and photon imaging...
  82. pmc Calmodulin mediates Ca2+-dependent modulation of M-type K+ channels
    Nikita Gamper
    Department of Physiology, MS 7756, University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Drive, San Antonio, TX 78229, USA
    J Gen Physiol 122:17-31. 2003
    To quantify the modulation of KCNQ2/3 current by [Ca2+]i and to test if calmodulin (CaM) mediates this action, simultaneous whole-cell recording and Ca2+ imaging was performed on CHO cells expressing KCNQ2/3 channels, either alone, or ..
  83. ncbi Transcriptomic comparison of two Entamoeba histolytica strains with defined virulence phenotypes identifies new virulence factor candidates and key differences in the expression patterns of cysteine proteases, lectin light chains, and calmodulin
    Paul H Davis
    Department of Medicine, Washington University School of Medicine, St Louis, MO, USA
    Mol Biochem Parasitol 151:118-28. 2007
    ..histolytica HM-1:IMSS genome. We find extensive differences between the two strains, including distinct patterns of gene expression of cysteine proteinases, AIG family members, and lectin light chains...
  84. ncbi Beta-adrenergic enhancement of sarcoplasmic reticulum calcium leak in cardiac myocytes is mediated by calcium/calmodulin-dependent protein kinase
    Jerald Curran
    Rush University Medical Center, Chicago, IL 60611, USA
    Circ Res 100:391-8. 2007
    ..increased SR Ca leak in intact rabbit ventricular myocytes and whether this depends on protein kinase A or Ca/calmodulin-dependent protein kinase II (CaMKII) activity...
  85. ncbi Controlling the cell cycle: the role of calcium/calmodulin-stimulated protein kinases I and II
    Kathryn A Skelding
    The University of Newcastle, Callaghan, New South Wales, Australia
    Cell Cycle 10:631-9. 2011
    Many studies have implicated Ca²+ and calmodulin (CaM) as regulators of the cell cycle...
  86. pmc A modular switch for spatial Ca2+ selectivity in the calmodulin regulation of CaV channels
    Ivy E Dick
    Calcium Signals Laboratory, Departments of Biomedical Engineering and Neuroscience, The Johns Hopkins University School of Medicine, Ross Building, Room 713, 720 Rutland Avenue, Baltimore, Maryland 21205, USA
    Nature 451:830-4. 2008
    Ca2+/calmodulin-dependent regulation of voltage-gated CaV1-2 Ca2+ channels shows extraordinary modes of spatial Ca2+ decoding and channel modulation, vital for many biological functions...
  87. ncbi Calmodulin-dependent activation of the epithelial calcium-dependent chloride channel TMEM16A
    Yuemin Tian
    Institut fur Physiologie, Universitat Regensburg, Universitätsstraße 31, D 93053 Regensburg, Germany
    FASEB J 25:1058-68. 2011
    ..In contrast, the Ca(2+) binding protein calmodulin appears indispensable and interacts physically with TMEM16A...
  88. ncbi Calmodulin dependence of presynaptic metabotropic glutamate receptor signaling
    V O'Connor
    Department of Neurochemistry, Max Planck Institute for Brain Research, Deutschordenstrasse 46, 60528 Frankfurt, Germany
    Science 286:1180-4. 1999
    ..A subdomain in the intracellular carboxyl-terminal tail of group III mGluRs binds calmodulin and heterotrimeric guanosine triphosphate-binding protein (G protein) betagamma subunits in a mutually exclusive ..
  89. ncbi Novel aspects of calmodulin target recognition and activation
    Stefan W Vetter
    Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA, USA
    Eur J Biochem 270:404-14. 2003
    Several crystal and NMR structures of calmodulin (CaM) in complex with fragments derived from CaM-regulated proteins have been reported recently and reveal novel ways for CaM to interact with its targets...
  90. ncbi Phospholipase C, calcium, and calmodulin are critical for alpha4beta1 integrin affinity up-regulation and monocyte arrest triggered by chemoattractants
    Sharon J Hyduk
    Department of Laboratory Medicine and Pathobiology, University of Toronto, ON, Canada
    Blood 109:176-84. 2007
    ..inositol 1,4,5-triphosphate receptors, increased intracellular calcium, influx of extracellular calcium, and calmodulin, suggesting that this signaling pathway is required for alpha4 integrins to assume a high-affinity conformation...
  91. ncbi Local control of mitochondrial membrane potential, permeability transition pore and reactive oxygen species by calcium and calmodulin in rat ventricular myocytes
    Keiichi Odagiri
    Division of Cardiology, Internal Medicine III, Hamamatsu University School of Medicine, Higashi Ward, Hamamatsu, Japan
    J Mol Cell Cardiol 46:989-97. 2009
    b>Calmodulin (CaM) and Ca(2+)/CaM-dependent protein kinase II (CaMKII) play important roles in the development of heart failure...
  92. ncbi A novel target recognition revealed by calmodulin in complex with Ca2+-calmodulin-dependent kinase kinase
    M Osawa
    Molecular Chemistry Research, Chemistry Laboratories, Institute for Drug Discovery Research, Yamanouchi Pharmaceutical Co, Ltd, Tsukuba 305 8585, Japan
    Nat Struct Biol 6:819-24. 1999
    The structure of calcium-bound calmodulin (Ca2+/CaM) complexed with a 26-residue peptide, corresponding to the CaM-binding domain of rat Ca2+/CaM-dependent protein kinase kinase (CaMKK), has been determined by NMR spectroscopy...
  93. ncbi Regulation of aquaporin water permeability in the lens
    Kulandaiappan Varadaraj
    Department of Physiology and Biophysics, State University of New York at Stony Brook, Stony Brook, New York, USA
    Invest Ophthalmol Vis Sci 46:1393-402. 2005
    ..To examine Ca(2+)- and pH-mediated regulation of water permeability of endogenously expressed aquaporin (AQP)0 in lens fiber cells and AQP1 in lens epithelial cells...
  94. ncbi A calmodulin-binding/CGCG box DNA-binding protein family involved in multiple signaling pathways in plants
    Tianbao Yang
    Laboratory of Plant Molecular Biology and Physiology, Department of Horticulture, Washington State University, Pullman, Washington 99164 6414, USA
    J Biol Chem 277:45049-58. 2002
    We reported earlier that the tobacco early ethylene-responsive gene NtER1 encodes a calmodulin-binding protein (Yang, T., and Poovaiah, B. W. (2000) J. Biol. Chem. 275, 38467-38473)...
  95. pmc Tuberculosis toxin blocking phagosome maturation inhibits a novel Ca2+/calmodulin-PI3K hVPS34 cascade
    Isabelle Vergne
    Department of Molecular Genetics and Microbiology, University of New Mexico Health Sciences Center, 915 Camino de Salud, NE, Albuquerque, NM 87131, USA
    J Exp Med 198:653-9. 2003
    ..tuberculosis, but not from avirulent mycobacteria, blocked cytosolic Ca2+ increase. Ca2+ and calmodulin were required for a newly uncovered Ca2+/calmodulin phosphatidylinositol (PI)3 kinase hVPS34 cascade, essential ..
  96. pmc Nonconserved Ca(2+)/calmodulin binding sites in Munc13s differentially control synaptic short-term plasticity
    Noa Lipstein
    Department of Molecular Neurobiology, Max Planck Institute of Experimental Medicine, Gottingen, Germany
    Mol Cell Biol 32:4628-41. 2012
    ..During high synaptic activity, Munc13-1 and its closely related homolog, ubMunc13-2, bind Ca(2+)/calmodulin, resulting in enhanced priming activity and in changes of short-term synaptic plasticity characteristics...
  97. ncbi Calmodulin activity and cAMP signalling modulate growth and apical secretion in pollen tubes
    Cláudia Rato
    Departamento de Biologia Vegetal, Faculdade de Ciencias de Lisboa, ICAT, Campo Grande, 1749 016 Lisbon, Portugal
    Plant J 38:887-97. 2004
    Our present understanding implicates both calmodulin (CaM) and 3',5'-cyclicAMP (cAMP) in the regulation of pollen tube growth...
  98. ncbi Dynamic control of synaptic vesicle replenishment and short-term plasticity by Ca(2+)-calmodulin-Munc13-1 signaling
    Noa Lipstein
    Department of Molecular Neurobiology, Max Planck Institute of Experimental Medicine, 37075 Gottingen, Germany
    Neuron 79:82-96. 2013
    ..We studied calyces of Held in knockin mice that express a Ca(2+)-Calmodulin insensitive Munc13-1(W464R) variant of the synaptic vesicle priming protein Munc13-1...
  99. pmc The effect of macromolecular crowding, ionic strength and calcium binding on calmodulin dynamics
    Qian Wang
    Department of Physics, University of Houston, Houston, Texas, United States of America
    PLoS Comput Biol 7:e1002114. 2011
    The flexibility in the structure of calmodulin (CaM) allows its binding to over 300 target proteins in the cell...
  100. ncbi Molecular cloning and characterization of retinal photoreceptor guanylyl cyclase-activating protein
    K Palczewski
    Department of Ophthalmology, University of Washington, Seattle 98195
    Neuron 13:395-404. 1994
    ....
  101. pmc Calcium and calmodulin-mediated regulation of gene expression in plants
    Min Chul Kim
    Division of Applied Life Science BK21 program, Plant Molecular Biology and Biotechnology Research Center and Environmental Biotechnology National Core Research Center, Gyeongsang National University, Jinju 660 701, Korea
    Mol Plant 2:13-21. 2009
    ..b>Calmodulin (CaM) is the predominant Ca2+ sensor and plays a crucial role in decoding the Ca2+ signatures into proper ..

Research Grants75

  1. Antiarrhythmic Mechanisms in the Hearts of Hibernating Mammals
    Lai Hua Xie; Fiscal Year: 2013
    ..Our recent studies have revealed that H2O2 induces EAD, DAD and TA via activation of the Ca/Calmodulin-dependent kinase II (CaMKII) pathway in rabbit ventricular myocytes...
  2. TARGETING THE MARCKS-FAMILY PROTEINS TO INHIBIT NEUTROPHIL MIGRATION
    Mary K Sheats; Fiscal Year: 2013
    ..known cell membrane and actin binding proteins regulated by protein kinase C (PKC) phosphorylation and calcium/calmodulin binding...
  3. Purkinje Origin of Ischemic Ventricular Tachycardia and Fibrillation
    James B Martins; Fiscal Year: 2013
    ..ROS may also result in oxidized calcium calmodulin kinase II (CaMKII), a fundamental contributor to VT/VF including reentry in small and large animal models...
  4. Hypertension and Collagen: Effect of Ac-SDKP
    Nour Eddine Rhaleb; Fiscal Year: 2010
    ..the effects of calcium ionophores or EGF on p42/44 MAPK and collagen synthesis;3) whether PLC, EGFR, cSrc, calmodulin kinase or IP3 inhibitors attenuate MAPK activity and collagen synthesis to the same extent as Ac-SDKP in ..
  5. Gene Transcription in SLE
    George C Tsokos; Fiscal Year: 2012
    ..We also demonstrated that anti-CD3/TCR autoantibodies activate calcium/calmodulin kinase IV (CaMKIV) which is found primarily in the nucleus where it phosphorylates CREM and histones...
  6. Regulation of Cardiac Kv Channel Expression
    Edwin S Levitan; Fiscal Year: 2013
    ..Our recent studies show that AII acts via endosomes and CamKII (calmodulin dependent protein kinase II) to induce biphasic activation of p38 kinase...
  7. Molecular physiology of voltage-gated Ca2+ channels
    Jian Yang; Fiscal Year: 2013
    ..regulated by diverse molecules and pathways, including protein kinases and phosphatases, G proteins, calcium and calmodulin, SNAREs, and phospholipids...
  8. Neuroendocrine Mechanisms and Therapeutics in an Animal Model of PTSD
    DAVID MARK DIAMOND; Fiscal Year: 2013
    ..influence of predator-induced fear conditioning to activate molecular signalling molecules, including calcium/calmodulin- dependent kinase II (CaMKII) and cAMP-response element binding protein (CREB), in response to the traumatic ..
  9. Development of an effective therapy against post-exposure Anthrax
    Maurizio Pellecchia; Fiscal Year: 2010
    ..exotoxin comprised of protective antigen (PA), lethal factor (LF, a Zn2+ dependant metalloproteinase) and calmodulin-activated edema factor adenylate cyclase (EF)...
  10. SFRP2 and NFAT are therapeutic targets in angiosarcoma
    NANCY K DEMORE; Fiscal Year: 2013
    ..NFAT nuclear localization is dependent on a dynamic import-export balance between the activity of the Ca2+/calmodulin-dependent phosphatase, calcineurin, and the activity of serine/threonine kinases(20)...
  11. Neurotrophins and Neuropeptides in Colon and Bladder Hypersensitivity
    Liya Qiao; Fiscal Year: 2013
    ..Aims are proposed to examine the regulatory mechanism and the targets of the PLC?-Ca2+ pathways including Ca2+/calmodulin-dependent protein kinase (CaMK)II, cAMP-response element binding protein (CREB), calcitonin gene-related peptide ..
  12. Autophagy in Brain Tumor
    Jin Ming Yang; Fiscal Year: 2013
    ..Furthermore, we found that eEF-2 kinase, a unique calmodulin- dependent enzyme that inhibits protein elongation and is overexpressed in glioma, is critically linked to ..
  13. The Effect Of Deafness Associated Mutations on MyosinVIIA Function
    Eva Forgacs; Fiscal Year: 2010
    ..shaker mutants of the myosin VIIA will be expressed in a baculovirus/insect expression system along with the calmodulin light chain...
  14. Role of spectrin/ankyrin-G complex in myocyte signaling and cardiac excitability
    Peter J Mohler; Fiscal Year: 2013
    ..Ca2+/calmodulin-dependent protein kinase II (CaMKII) is a multifunctional signaling molecule with critical roles in cardiac ..
  15. MECHANISMS OF MALADAPTATION IN HEART FAILURE
    Howard A Rockman; Fiscal Year: 2013
    ..post-MI remodeling resulting in a decline in cardiac function, which is associated with activation of calcium/calmodulin-dependent protein kinase II (CaMKII)...
  16. HEREDITARY DEFECTS IN HUMAN SODIUM CHANNELS
    Alfred L George; Fiscal Year: 2013
    ..1 C-terminus having conserved Ca2+/calmodulin regulatory elements to test the hypothesis that these alleles affect channel function by altering the response ..
  17. Molecular mechanism of regulation of mI(CAT) in intestinal smooth muscle cells
    Michael X Zhu; Fiscal Year: 2012
    ..the G1i/o or G23 subunits, or both, with the TRPC4 protein, and Ca2+ exerts multiple regulatory actions through calmodulin binding at distinct sites of the channel molecule...
  18. Regulation of TRP channels and visual transduction
    Craig Montell; Fiscal Year: 2013
    ..role in vivo, namely to regulate the TRP channel by preventing its interaction with inhibitory calcium/calmodulin. As part of a long-term goal to define the proteins that participate in PI signaling in photoreceptor cells, we ..
  19. Histone Deacetylation Regulates Growth Plate Development
    Lei Wei; Fiscal Year: 2013
    ..degradation, and translational repression of HDAC4 control chondrocyte differentiation and are regulated by Ca2+/calmodulin kinase IV (CaMKIV), p38 MAP kinase, and microRNA-1 respectively at different zone in the growth plate...
  20. A Phase II Trial of Levodopa in Angelman Syndrome
    Wen Hann Tan; Fiscal Year: 2013
    ..Currently, there is no cure for AS and treatment is largely supportive. Calcium/calmodulin-dependent kinase type 2 (CaMKII) is an enzyme in post-synaptic neurons that is involved in the formation of ..
  21. AMP-activated protein kinase in the ischemic heart
    Lawrence H Young; Fiscal Year: 2012
    ..cardiomyocte-derived MIF during ischemia, 2) define the roles of the tumor suppressing kinase LKB1 and calcium calmodulin-activated protein kinase kinase (CaMKK2) in AMPK activation in the ischemic heart and 3) develop therapeutic ..
  22. Understanding the biology of schistosomes in response to praziquantel
    Charles Cunningham; Fiscal Year: 2013
    ..Among the first potential pathway components to be targeted will be calmodulin, myosin light chain kinase and death associated protein kinase which may play a role in PZQ associated ..
  23. Reciprocal Control of Ca2+ Channels by Anchored Protein Kinase A and Calcineurin
    William A Sather; Fiscal Year: 2010
    ..2, AKAP79, PKA regulatory and catalytic subunits, CaN and its Ca2+-dependent activator calmodulin). These fluorescent fusion constructs will be transfected into HEK293 cells or cultured rat hippocampal neurons...
  24. Isoform specific effects of the autoinhibitory element and the C-terminus of nitr
    JOHN SALERNO; Fiscal Year: 2009
    ..The most important of several inputs is calcium/calmodulin (Ca+2/CaM), but NO synthesis is also influenced by phosphorylation and protein-protein interactions...
  25. Mechanism and functional role of AKAP79/150 in M current control
    Mark S Shapiro; Fiscal Year: 2012
    ..One such AKAP, AKAP79/150 interacts with protein kinase A, protein kinase C (PKC), calmodulin (CaM), calcineurin (CaN), ion channels such as M-type (KCNQ, Kv7) K+ channels, L-type Ca2+ channels and G ..
  26. Hepatitis B virus X protein modulation of calcium signaling in HCC development
    JESSICA GARNER; Fiscal Year: 2013
    ..and has been shown to play a critical role in many aspects of cancer development;the Ca2+-binding protein calmodulin (CaM) is thought to be a major contributor to cancer development and is required for cell proliferation...
  27. Endogenous regulators of inflammation in liver ischemia/reperfusion
    Allan Tsung; Fiscal Year: 2013
    ..We will demonstrate the mechanisms by which calcium/ calmodulin-dependent protein kinase II control the acetylation status and subsequent release of HMGB1 through modification ..
  28. Molecular mechanisms of neural plasticity in drug addiction
    LAURA NICOLE SMITH; Fiscal Year: 2010
    ..Initial focus will be on whether regulator of calmodulin signaling (RCS), a small neuronal phosphoprotein, is required for this process using neurons cultured from RCS-..
  29. Physiology of Class III PI 3-kinase Signaling 2
    Jonathan M Backer; Fiscal Year: 2013
    ..Finally, Aim 4 uses novel mutants that selectively disrupt hVps34 binding to calmodulin and hVps15 binding to Rab5 and Rab7, block hVps34 degradation, and abolish hVps34 lipid kinase activity while ..
  30. CaMKII in Sinus Node Physiology and Disease
    Mark E Anderson; Fiscal Year: 2013
    ..provided by applicant): During the initial funding period, our group identified the multifunctional Ca2+ and calmodulin-dependent protein kinase II (CaMKII) as a necessary signal for physiological, 'fight or flight', ..
  31. CaMKII in global cerebral ischemia
    Paco S Herson; Fiscal Year: 2013
    ..stimulation of NMDAr triggers a series of Ca2+-dependent signaling pathways, including activation of Ca2+/calmodulin-dependent protein kinase II (CaMKII)...
  32. Exercise Induced Biochemical and Anatomical Adaptations
    John O Holloszy; Fiscal Year: 2013
    ..This effect of Ca2. is mediated by activation of Ca2*-calmodulin dependent protein kinases (CAMKs)...
  33. Control of AMPA receptor function by phosphorylation
    Stephen F Traynelis; Fiscal Year: 2013
    ..Phosphorylation by protein kinase C (PKC) or Ca2+/calmodulin dependent kinase II (CamKII) of an intracellular serine residue (Ser831) located on the GluR1 subunit enhances ..
  34. Role of Ca2+/Calcineurin Signaling in S. cerevisiae
    Martha S Cyert; Fiscal Year: 2013
    ..by applicant): Calcineurin (CN) is a highly conserved ser/thr protein phosphatase that is regulated by Ca2+ and calmodulin, and thus mediates Ca2+-dependent changes in phosphorylation in vivo...
  35. Role of CaMKII Phosphorylation of RyR in Pathological &Physiological Hypertrophy
    Jonathan L Respress; Fiscal Year: 2011
    ..Chronic Ca2+/calmodulin-dependent protein kinase (CaMKII) phosphorylation at serine 2814 (S2814) on RyR2CaMKII has been recognized as a ..
  36. Regulation of Cardiac Calcium Channels by an Autoinhibitory Signalling Complex
    William A Catterall; Fiscal Year: 2013
    ..regulated by voltage-dependent potentiation and Ca-dependent facilitation, and phosphorylation by PKA and Ca/calmodulin-dependent protein kinase II (CaMKII) is involved in this regulation...
  37. FKBP Interaction with RYR Calcium Channels in Heart
    Donald M Bers; Fiscal Year: 2013
    ..distances and orientation between critical sites in the RyR2 complex in situ using FRET between FKBP and calmodulin, RyR2 itself and other complex components, and 4) measure RyR FKBP12/12...
  38. Molecular Mechanisms of Host Function Exploitation by Type IV effectors of Legion
    Zhao Qing Luo; Fiscal Year: 2013
    ..Second, the toxicity of SidJ to yeast under overexpression condition was due to the titration of calmodulin. In an independent line of study aiming at analyzing the impact of Dot/Icm substrates on eukaryotic cells, we ..
  39. Molecular Dissection of Calmodulin Domain Functions
    Madeline A Shea; Fiscal Year: 2013
    ..Most members of the NaV family of channels respond to calcium via regulation by calmodulin (CaM), an essential eukaryotic calcium sensor that is comprised of two highly homologous domains (N and C) ..
  40. Skeletal muscle ATP-sensitive potassium channels determine bodily energy balance
    Leonid Zingman; Fiscal Year: 2013
    ..turnover, causing increased energy consumption and activation of protein kinase B (Akt) by calcium dependent calmodulin kinase...
  41. Functional Consequences of FHC-linked RLC Mutations.
    Danuta Szczesna-Cordary; Fiscal Year: 2012
    ..We further hypothesize that an FHC induced pathological cardiac phenotype can be rescued by Ca2+-calmodulin activated MLCK phosphorylation of the RLC-mutated myocardium...
  42. Properties of Clca Channels in Vascular Myocytes
    Normand Leblanc; Fiscal Year: 2009
    ..is focused on elucidating the mechanisms involved in the regulation by phosphorylation mechanisms involving calmodulin-dependent kinase II (CaMKII) and serine/threonine phosphatases (Calcineurin and PP2A) of a Cl- channel activated ..
  43. Ryanodine receptor in cardiac hypertrophy and heart failure
    GERHARD W MEISSNER; Fiscal Year: 2013
    ..The RyR2 ion channel is composed of four RyR2 560 kDa subunits that bind calmodulin (CaM), and four small 12.6 kDa FK506 binding proteins...
  44. Roles of voltage sensor, S100A1 and calmodulin in skeletal muscle Ca2+ signaling
    Martin F Schneider; Fiscal Year: 2013
    ..release activation are poorly understood, and the roles of various modulatory molecules, including S100A1 and calmodulin (CaM) are not clear...
  45. Control of KATP channel expression by CaMKII: role in heart failure resistance
    DENICE HODGSON-ZINGMAN; Fiscal Year: 2013
    ..data, we hypothesize that membrane KATP channel expression is coupled with overall cardiac function by calcium/calmodulin dependent protein kinase II (CaMKII)...
  46. Signal transduction mechanisms to myosin phosphatase
    James T Stull; Fiscal Year: 2013
    ..These movements respond to increased cytosolic Ca2+ concentrations ([Ca2+]i) and activation of Ca2+/calmodulin (CaM)-dependent myosin light chain kinase (MLCK)...
  47. Role of Calcineurin in Fungal Virulence
    Joseph Heitman; Fiscal Year: 2009
    ..The central element of this virulence pathway is the calcium-calmodulin activated protein phosphatase calcineurin, which is the molecular target of the immunosuppressive antifungal ..
  48. HTS ASSAYS FOR INHIBITORS OF PROTEIN INTERACTIONS INCLUDING MLCK1-FKBP8
    Jerrold R Turner; Fiscal Year: 2012
    ..However, the myosin light chain kinase gene MYLK encodes both long and short MLCK, which share catalytic and calmodulin-binding regulatory domains, and therefore suitable reagents for MLCK1 inhibition in vivo are not available...
  49. Calcium Sensing Proteins in Depression
    Yogesh Dwivedi; Fiscal Year: 2013
    ..The varied effects of Ca2+ are mediated through Ca2+ sensing proteins;one highly characterized member is calmodulin (CaM). Recently, two different subfamilies of Ca2+ sensing proteins have been identified, i.e...
  50. Regulation of BDNF Expression by Calcium-stimulated Protein Kinases
    Hongbing Wang; Fiscal Year: 2010
    ..Although the role of PKA, ERK and calmodulin-dependent kinases (CaMK) were implicated in CREB-mediated transcription, their function in regulating native ..
  51. Signal transduction and gene induction in lymphocytes
    Anjana Rao; Fiscal Year: 2013
    ..In Aim 1, we will define the biochemical features of the scaffold complex containing NFAT, NRON, IQGAP1, calmodulin, NFAT kinases and importins in resting and activated T cells, asking if components of the complex are involved ..
  52. MUSCLE PROTEIN TURNOVER AND AMINO ACID UPTAKE IN SEPSIS
    Per Olof J Hasselgren; Fiscal Year: 2010
    ..Specific Aim 1 tests the hypothesis that sepsis and glucocorticoids increase muscle calcium levels and calcium-calmodulin protein kinase II (CaMK II) and calpain activities...
  53. Molecular Mechanisms in Retinal Degeneration
    Debora B Farber; Fiscal Year: 2011
    ..the effects of GC-activating proteins, nitric oxide and carbon monoxide;the participation of rod, cone and Ca2+/calmodulin-dependent PDEs, and the role of guanine deaminase, which converts cGMP into cXMP...
  54. Functional analysis of the CAMTA Transcription Factors
    Hong Sheng Li; Fiscal Year: 2013
    ..We have previously demonstrated that dCAMTA, a transcription factor responding to the Ca2+ sensor calmodulin, is indispensable for rapid deactivation of the light-stimulated GPCR rhodopsin in the Drosophila eye...
  55. Regulation of Drosophila arrestins in light adaptation
    Bih Hwa Shieh; Fiscal Year: 2012
    ..Both Arr1 and Arr2 undergo light-dependent phosphorylation, and Arr2 was shown phosphorylated by Ca2+/calmodulin-dependent protein kinase II (CaMKII) in vitro...
  56. Chemosensing in the Gastrointestinal Tract
    Catia Sternini; Fiscal Year: 2010
    ..whether intraluminal T2R agonists activate endocrine cells in situ using immunohistochemistry for phosphorylated calmodulin dependent kinase 2 (CAMK2) as a marker for intracellular Ca2+ elevation...
  57. Site-Directed Oxidative Modification of Muscle Protein Structural Dynamics
    David D Thomas; Fiscal Year: 2013
    ..The present proposal focuses on the effect of methionine oxidation in two key muscle proteins - calmodulin (CaM), in its role as regulator of the calcium release channel (ryanodine receptor, RyR) and myosin, in its role ..
  58. The Role of DRP-1 and Mitochondrial Fission in Pulmonary Arterial Hypertension
    Stephen L Archer; Fiscal Year: 2013
    ..Elevated cytosolic calcium in PAH also promotes DRP-1-mediated fission by: a) activation of calmodulin kinase and b) calcineurin-mediated dephosphorylation of an inhibitory form of DRP- 1 (Phos-Ser637)...
  59. DAPK1 regulation of NMDA receptors in ischemic neuronal death
    NICOLAS GUILLERMO BAZAN; Fiscal Year: 2012
    ..DAPK1 is one member of Ca2+/calmodulin (CaM)-dependent serine/threonine kinase family and functions as a critical mediator of cell death...
  60. Regulation of ClC-3 in Human Malignant Glioma
    Vishnu Anand Cuddapah; Fiscal Year: 2012
    ..Several lines of evidence suggest that ClC-3 may be regulated by Ca2+/calmodulin-dependent protein kinase II, a Ca2+-sensitive kinase...
  61. AKAP Regulation of Neuronal L-type Calcium Channel Signaling to the Nucleus
    MARK L DELL'ACQUA; Fiscal Year: 2013
    ..2 through a modified leucine zipper (LZ) motif and anchors the cAMP-dependent protein kinase (PKA) and Ca2+-calmodulin (CaM)-activated protein phosphatase-2B (calcineurin;CaN), as an essential regulator of neuronal LTCC currents ..
  62. Multi-scale Systems Model of Murine Heart Failure
    Andrew D McCulloch; Fiscal Year: 2013
    ..Ca2+-calmodulin dependent protein kinase (CaMKII) is upregulated and more active in heart failure, and is a key regulator of ..
  63. DIARRHEAL DISEASES: CALCIUM AND PHOSPHORYLATION
    GEOFFREY SHARP; Fiscal Year: 1991
    ..We will concentrate on the role of Ca++ and calmodulin dependent phosphorylation in the regulation of active ileal electrolyte transport and will study:- 1...
  64. CALMODULIN KINASE II AND ION TRANSPORT IN THE KIDNEY
    Rochelle Hanley; Fiscal Year: 1992
    Calcium-calmodulin dependent protein kinase II) (CaM-KII) is a widely distributed enzyme with a broad substrate specificity...
  65. Intervention against anthrax edema factor (EF)
    Wei Jen Tang; Fiscal Year: 2005
    ..Edema factor (EF), the catalytic component of EdTx has calmodulin-activated adenylyl cyclase activity to raise the uncontrolled intracellular cAMP level...
  66. GONOCOCCAL PIA--ROLE IN INVASION OF FALLOPIAN TUBE CELL
    GARY GORBY; Fiscal Year: 1999
    ..It can transfer spontaneously from GC to lipid bilayers and human cells. It is able to bind calmodulin, but the impact of this on pathogenesis is unknown...
  67. CALMODULIN DEPENDENT KINASE AND SYNAPTIC FUNCTION
    MARY VALLANO; Fiscal Year: 1992
    A Type II calcium/calmodulin-dependent protein kinase (CaM kinase II) that phosphorylates cytoskeletal proteins as major substrates in highly concentrated in neurons, including the postsynaptic density (PSD)...
  68. MECHANISMS OF OCCUPATIONAL NEUROPATHIES
    MOHAMED ABOU DONIA; Fiscal Year: 1999
    ..The investigators have demonstrated that central to the pathogenesis of OPIDN is an anomalous increase in Ca2+/calmodulin-dependent kinase- mediated phosphorylation of the cytoskeletal proteins, i.e...
  69. IONIC CONTROL OF CARDIAC MUSCLE CONTRACTION: EC COUPLING
    Donald Bers; Fiscal Year: 2004
    ..project focuses on 1) interactions between dihydropyridine receptor (DHPR) and ryanodine receptors (RyR), 2) Ca-Calmodulin dynamics in intact cells, 3) the mechanism of frequency-dependent acceleration of relaxation and 4) some key ..
  70. Molecular study of calcium channels in fungal pathogens.
    ANGELA GELLI; Fiscal Year: 2007
    ..to proliferate within host cells and subsequently promote disease, fungal pathogens require an active calcium-calmodulin-dependent signaling cascade...
  71. PHASE-RESOLVED FLUORESCENCE STUDIES OF PROTEIN DYNAMICS
    PATRICIA O HARA; Fiscal Year: 1993
    ..of this research is the experimental observation and description of macromolecular dynamics in the protein calmodulin. These motions will be examined by intramolecular nonradiative energy transfer using conventional and frequency ..
  72. CALCIUM & CALCIUM BINDING PROTEINS IN THE EPIDERMIS
    JANET FAIRLEY; Fiscal Year: 1990
    ..We propose to define the role of free intracellular calcium, the calcium-binding protein calmodulin, and extracellular calcium in regulation of epidermal proliferation and differentiation...
  73. METABOLIC CONTROL OF INSULIN EXOCYTOSIS
    Christopher Rhodes; Fiscal Year: 2005
    ..In beta-cells, Rab3A interacts with calmodulin in a GTP- and Ca2+-dependent manner...
  74. TAMOXIFEN MODULATES OSTEOCLAST CA++/CALMODULIN SIGNALING
    Jay McDonald; Fiscal Year: 2001
    ..the first funding period, we determined that tamoxifen inhibits mature osteoclast activity by disrupting Ca/2+/calmodulin and protein kinase C signaling cascades by a mechanism that is independent of estrogen...
  75. REGULATION OF DYNEIN-DRIVEN FLAGELLAR MOTILITY
    Pinfen Yang; Fiscal Year: 2009
    ..Among the key molecules are two constitutive spoke proteins, RSP2 and calmodulin, that are essential for motility...