gq g11 gtp binding protein alpha subunits

Summary

Summary: A family of heterotrimeric GTP-binding protein alpha subunits that activate PHOSPHOLIPASE C dependent signaling pathways. The Gq-G11 part of the name is also spelled Gq/G11.

Top Publications

  1. ncbi G protein pathways
    Susana R Neves
    Department of Pharmacology and Biological Chemistry, Mount Sinai School of Medicine, New York, NY 10029, USA
    Science 296:1636-9. 2002
  2. ncbi Potent activation of RhoA by Galpha q and Gq-coupled receptors
    Hiroki Chikumi
    Oral and Pharyngeal Cancer Branch, NIDCR National Institutes of Health, 9000 Rockville Pike, Bethesda, MD 20892 4330, USA
    J Biol Chem 277:27130-4. 2002
  3. pmc Genetic interactions between polymorphisms that affect gene expression in yeast
    Rachel B Brem
    Program in Computational Biology, Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue N, M2 B876, Seattle, Washington 98109, USA
    Nature 436:701-3. 2005
  4. ncbi Distinct roles for Galpha and Gbetagamma in regulating spindle position and orientation in Caenorhabditis elegans embryos
    M Gotta
    Wellcome CRC Institute, Tennis Court Road, Cambridge, CB2 1QR, UK
    Nat Cell Biol 3:297-300. 2001
  5. ncbi Distinct roles of Galpha(q) and Galpha11 for Purkinje cell signaling and motor behavior
    J Hartmann
    Institut fur Physiologie, Ludwig Maximilians Universitat, 80336 Munich, Germany
    J Neurosci 24:5119-30. 2004
  6. ncbi RIC-8 (Synembryn): a novel conserved protein that is required for G(q)alpha signaling in the C. elegans nervous system
    K G Miller
    Program in Molecular and Cell Biology, Oklahoma Medical Research Foundation, Oklahoma City 73104, USA
    Neuron 27:289-99. 2000
  7. ncbi eat-11 encodes GPB-2, a Gbeta(5) ortholog that interacts with G(o)alpha and G(q)alpha to regulate C. elegans behavior
    M Robatzek
    Department of Genetics, The University of Washington, Seattle, WA 98195, USA
    Curr Biol 11:288-93. 2001
  8. ncbi Muscarinic inhibition of calcium current and M current in Galpha q-deficient mice
    J E Haley
    Wellcome Laboratory for Molecular Pharmacology, Department of Pharmacology, University College London, London, WC1E 6BT, United Kingdom
    J Neurosci 20:3973-9. 2000
  9. ncbi Modulation of EGF receptor-mediated vulva development by the heterotrimeric G-protein Galphaq and excitable cells in C. elegans
    Nadeem Moghal
    Howard Hughes Medical Institute and Division of Biology, California Institute of Technology, Pasadena, CA 91125, USA
    Development 130:4553-66. 2003
  10. pmc Caenorhabditis elegans Galphaq regulates egg-laying behavior via a PLCbeta-independent and serotonin-dependent signaling pathway and likely functions both in the nervous system and in muscle
    Carol A Bastiani
    Howard Hughes Medical Institute, California Institute of Technology, Pasadena, California 91125, USA
    Genetics 165:1805-22. 2003

Detail Information

Publications190 found, 100 shown here

  1. ncbi G protein pathways
    Susana R Neves
    Department of Pharmacology and Biological Chemistry, Mount Sinai School of Medicine, New York, NY 10029, USA
    Science 296:1636-9. 2002
    ..These cellular processes in turn regulate systemic functions such as embryonic development, gonadal development, learning and memory, and organismal homeostasis...
  2. ncbi Potent activation of RhoA by Galpha q and Gq-coupled receptors
    Hiroki Chikumi
    Oral and Pharyngeal Cancer Branch, NIDCR National Institutes of Health, 9000 Rockville Pike, Bethesda, MD 20892 4330, USA
    J Biol Chem 277:27130-4. 2002
    ....
  3. pmc Genetic interactions between polymorphisms that affect gene expression in yeast
    Rachel B Brem
    Program in Computational Biology, Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue N, M2 B876, Seattle, Washington 98109, USA
    Nature 436:701-3. 2005
    ..Our results indicate that genetic interactions are widespread in the genetics of transcript levels, and that many QTLs will be missed by single-locus tests but can be detected by two-stage tests that allow for interactions...
  4. ncbi Distinct roles for Galpha and Gbetagamma in regulating spindle position and orientation in Caenorhabditis elegans embryos
    M Gotta
    Wellcome CRC Institute, Tennis Court Road, Cambridge, CB2 1QR, UK
    Nat Cell Biol 3:297-300. 2001
    ..Gbetagamma is important in regulating migration of the centrosome around the nucleus and hence in orientating the mitotic spindle. Galpha is required for asymmetric spindle positioning in the one-celled embryo...
  5. ncbi Distinct roles of Galpha(q) and Galpha11 for Purkinje cell signaling and motor behavior
    J Hartmann
    Institut fur Physiologie, Ludwig Maximilians Universitat, 80336 Munich, Germany
    J Neurosci 24:5119-30. 2004
    ..Our findings suggest an expression level-dependent action of Galpha(q) and Galpha11 for Purkinje cell signaling and assign specific roles of these two G(q) isoforms for motor coordination...
  6. ncbi RIC-8 (Synembryn): a novel conserved protein that is required for G(q)alpha signaling in the C. elegans nervous system
    K G Miller
    Program in Molecular and Cell Biology, Oklahoma Medical Research Foundation, Oklahoma City 73104, USA
    Neuron 27:289-99. 2000
    ..Exogenous application of phorbol esters or loss of DGK-1 (diacylglycerol kinase) rescues ric-8 mutant phenotypes. A genetic analysis suggests that RIC-8 functions upstream of, or in conjunction with, EGL-30 (G(q)alpha)...
  7. ncbi eat-11 encodes GPB-2, a Gbeta(5) ortholog that interacts with G(o)alpha and G(q)alpha to regulate C. elegans behavior
    M Robatzek
    Department of Genetics, The University of Washington, Seattle, WA 98195, USA
    Curr Biol 11:288-93. 2001
    ..These interactions provide a mechanism for the modulation of behavior by antagonistic G protein networks...
  8. ncbi Muscarinic inhibition of calcium current and M current in Galpha q-deficient mice
    J E Haley
    Wellcome Laboratory for Molecular Pharmacology, Department of Pharmacology, University College London, London, WC1E 6BT, United Kingdom
    J Neurosci 20:3973-9. 2000
    ....
  9. ncbi Modulation of EGF receptor-mediated vulva development by the heterotrimeric G-protein Galphaq and excitable cells in C. elegans
    Nadeem Moghal
    Howard Hughes Medical Institute and Division of Biology, California Institute of Technology, Pasadena, CA 91125, USA
    Development 130:4553-66. 2003
    ....
  10. pmc Caenorhabditis elegans Galphaq regulates egg-laying behavior via a PLCbeta-independent and serotonin-dependent signaling pathway and likely functions both in the nervous system and in muscle
    Carol A Bastiani
    Howard Hughes Medical Institute, California Institute of Technology, Pasadena, California 91125, USA
    Genetics 165:1805-22. 2003
    ..Our data indicate that there are multiple signaling pathways downstream of EGL-30 and that different pathways could predominate with respect to the regulation of different behaviors...
  11. ncbi Absence of pressure overload induced myocardial hypertrophy after conditional inactivation of Galphaq/Galpha11 in cardiomyocytes
    N Wettschureck
    Institute of Pharmacology, University of Heidelberg, Heidelberg, Germany
    Nat Med 7:1236-40. 2001
    ....
  12. ncbi A novel Galphaq/11-selective inhibitor
    Jun Takasaki
    Institute for Drug Discovery Research, Yamanouchi Pharmaceutical Co, Ltd, 21 Miyukigaoka, Tsukuba shi, Ibaraki 305 8585, Japan
    J Biol Chem 279:47438-45. 2004
    ..This novel Galpha(q/11)-selective inhibitor is a promising and powerful tool for studying Galpha(q/11) protein activation, Galpha(q/11) -coupled receptor signaling, and Galpha(q/11)-mediated biological events...
  13. ncbi Light-dependent subcellular translocation of Gqalpha in Drosophila photoreceptors is facilitated by the photoreceptor-specific myosin III NINAC
    Michelle A Cronin
    Department of Biology, Boston University, 5 Cummington Street, Boston, MA 02215, USA
    J Cell Sci 117:4797-806. 2004
    ....
  14. pmc The P2Y2 nucleotide receptor requires interaction with alpha v integrins to access and activate G12
    Zhongji Liao
    Department of Biochemistry, Life Sciences Center, University of Missouri Columbia, Columbia, MO 65211, USA
    J Cell Sci 120:1654-62. 2007
    ....
  15. ncbi Altered expression of G(q/11alpha) protein shapes mGlu1 and mGlu5 receptor-mediated single cell inositol 1,4,5-trisphosphate and Ca(2+) signaling
    Peter J Atkinson
    Department of Cell Physiology and Pharmacology, University of Leicester, Maurice Shock Medical Sciences Building, University Road, Leicester, LE1 9HN, UK
    Mol Pharmacol 69:174-84. 2006
    ..These experiments indicate that altering G(q/11)alpha expression levels differentially affects spatiotemporal aspects of IP(3) and Ca(2+) signaling mediated by the mGlu1 and mGlu5 receptors...
  16. ncbi Differential contribution of GTPase activation and effector antagonism to the inhibitory effect of RGS proteins on Gq-mediated signaling in vivo
    Thomas Anger
    Cardiovascular Division, Brigham and Women s Hospital and Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 279:3906-15. 2004
    ..RGS5 and RGS16 appear to depend on G protein inactivation, whereas GAP-independent mechanisms (such as effector antagonism) are sufficient to mediate the inhibitory effect of RGS2 and RGS3...
  17. pmc Interaction of G alpha(12) with G alpha(13) and G alpha(q) signaling pathways
    Jennifer L Gu
    Division of Biology, 147 75 California Institute of Technology, Pasadena, CA 91125, USA
    Proc Natl Acad Sci U S A 99:9352-7. 2002
    ..These data indicate that the G alpha(12)-mediated signaling pathway functionally interacts not only with the G alpha(13)- but also with the G alpha(q/11)-mediated signaling systems...
  18. pmc Regulation of light-dependent Gqalpha translocation and morphological changes in fly photoreceptors
    Mickey Kosloff
    Department of Biological Chemistry and the Kuhne Minerva Center for Studies of Visual Transduction, Institute of Life Sciences, The Hebrew University, Givat Ram, Jerusalem, 91904, Israel
    EMBO J 22:459-68. 2003
    ....
  19. pmc Galphaq binds to p110alpha/p85alpha phosphoinositide 3-kinase and displaces Ras
    Lisa M Ballou
    Department of Medicine, Division of Hematology, Stony Brook University, Stony Brook, NY 11794, USA
    Biochem J 394:557-62. 2006
    ..These results suggest that activation of G(q)-coupled receptors leads to increased binding of Galpha(q).GTP to some isoforms of PI3K, which might explain why these receptors inhibit this signalling pathway in certain cell types...
  20. ncbi Selective inhibition of Cav3.3 T-type calcium channels by Galphaq/11-coupled muscarinic acetylcholine receptors
    Michael E Hildebrand
    Michael Smith Laboratories, University of British Columbia, Vancouver, British Columbia V6T 1Z4
    J Biol Chem 282:21043-55. 2007
    ..3 channel are necessary and sufficient for complete M1 receptor-mediated channel inhibition and represent novel sites not previously implicated in T-type channel modulation...
  21. pmc Galphaq directly activates p63RhoGEF and Trio via a conserved extension of the Dbl homology-associated pleckstrin homology domain
    Rafael J Rojas
    Department of Pharmacology, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    J Biol Chem 282:29201-10. 2007
    ..This unique extension is conserved in the related Dbl-family members Trio and Kalirin and we show that the C-terminal Rho-specific DH-PH cassette of Trio is similarly activated by Galpha(q)...
  22. ncbi Cell signalling diversity of the Gqalpha family of heterotrimeric G proteins
    Katherine B Hubbard
    Department of Pharmacology, Emory University School of Medicine, 1510 Clifton Rd, Atlanta, GA 30322, USA
    Cell Signal 18:135-50. 2006
    ..Taken together, these findings demonstrate that Gqalpha, G11alpha, G14alpha and G15/16alpha regulate both overlapping and distinct signalling pathways, indicating that they are more functionally diverse than previously thought...
  23. ncbi beta-Arrestin 1 and Galphaq/11 coordinately activate RhoA and stress fiber formation following receptor stimulation
    William G Barnes
    Department of Biochemistry, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Biol Chem 280:8041-50. 2005
    ....
  24. ncbi G alpha 16 couples chemoattractant receptors to NF-kappa B activation
    M Yang
    Department of Pharmacology, College of Medicine, University of Illinois, 8356 South Walcott Avenue, Chicago, IL 60612, USA
    J Immunol 166:6885-92. 2001
    ..These results suggest a potential role of Galpha(16) in transcriptional regulation downstream of chemoattractant receptors...
  25. pmc Galphaq binds two effectors separately in cells: evidence for predetermined signaling pathways
    Urszula Golebiewska
    Department of Physiology and Biophysics, Stony Brook University, Stony Brook, New York 11794 8661, USA
    Biophys J 95:2575-82. 2008
    ..Preformed G-protein/effector complexes will limit the number of pathways that a given signal will take, which may simplify predictive models...
  26. ncbi Akt-mediated cardiomyocyte survival pathways are compromised by G alpha q-induced phosphoinositide 4,5-bisphosphate depletion
    Amy L Howes
    Department of Pharmacology, University of California, San Diego, La Jolla, California 92093, USA
    J Biol Chem 278:40343-51. 2003
    ..Akt phosphorylation and PIP2 levels decrease concomitantly. Our findings suggest that an Akt-mediated cell survival pathway is compromised by the diminished availability of PIP2 elicited by pathological levels of Gq activity...
  27. ncbi The roles of alpha IIb beta 3-mediated outside-in signal transduction, thromboxane A2, and adenosine diphosphate in collagen-induced platelet aggregation
    Moon J Cho
    Department of Microbiology and Molecular Cell Sciences, University of Memphis and the Division of Experimental Hematology, St Jude Children s Research Hospital, Memphis, TN, USA
    Blood 101:2646-51. 2003
    ..Furthermore, a high level of collagen can cause weak TxA2 and ADP-independent aggregation, but maximal aggregation induced by a high level of collagen requires TxA2 or secretion...
  28. ncbi G-protein-dependent and -independent pathways regulate proteinase-activated receptor-2 mediated p65 NFkappaB serine 536 phosphorylation in human keratinocytes
    Fui Goon Goh
    Strathclyde Institute of Pharmacy and Biomedical Sciences, Division of Physiology and Pharmacology, University of Strathclyde, 27 Taylor Street, Glasgow, G4 0NR, Scotland, UK
    Cell Signal 20:1267-74. 2008
    ..Overall these novel data demonstrate an IKK2-dependent, predominantly G-protein-independent pathway involved in PAR-2 regulation of NFkappaB phosphorylation in keratinocytes...
  29. ncbi Gbeta gamma isoforms selectively rescue plasma membrane localization and palmitoylation of mutant Galphas and Galphaq
    D S Evanko
    Department of Microbiology and Immunology and Kimmel Cancer Institute, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
    J Biol Chem 276:23945-53. 2001
    ....
  30. ncbi Receptor-dependent RhoA activation in G12/G13-deficient cells: genetic evidence for an involvement of Gq/G11
    Stephan Vogt
    Institute of Pharmacology, University of Heidelberg, Im Neuenheimer Feld 366, 69120 Heidelberg, Germany
    J Biol Chem 278:28743-9. 2003
    ..Our data clearly show that G12/G13 as well as Gq/G11 alone can couple GPCRs to the rapid activation of RhoA. Gq/G11-mediated RhoA activation occurs independently of phospholipase C-beta and appears to involve LARG...
  31. pmc Oncogenic mutations in GNAQ occur early in uveal melanoma
    Michael D Onken
    Department of Ophthalmology and Visual Sciences, Washington University School of Medicine, St Louis, Missouri, USA
    Invest Ophthalmol Vis Sci 49:5230-4. 2008
    ..An extensive search for such mutations was undertaken, focusing on the RAF/MEK/ERK pathway, which is often the target of initiating mutations in other types of cancer...
  32. ncbi RGS2 binds directly and selectively to the M1 muscarinic acetylcholine receptor third intracellular loop to modulate Gq/11alpha signaling
    Leah S Bernstein
    Department of Pharmacology, Emory University School of Medicine, 1510 Clifton Road, Atlanta, Georgia 30322, USA
    J Biol Chem 279:21248-56. 2004
    ....
  33. ncbi Functional selectivity of G protein signaling by agonist peptides and thrombin for the protease-activated receptor-1
    Joseph N McLaughlin
    Department of Pharmacology, Vanderbilt University Medical Center, Vanderbilt University, Nashville, Tennessee 37232, USA
    J Biol Chem 280:25048-59. 2005
    ..This model provides an estimate that peptide activation alters receptor/G protein binding to favor Galpha(q) activation over Galpha(12/13) by approximately 800-fold...
  34. ncbi T cell costimulation by chemokine receptors
    Barbara Molon
    Venetian Institute of Molecular Medicine and Department of Biomedical Science, University of Padua, 35100 Padua, Italy
    Nat Immunol 6:465-71. 2005
    ....
  35. ncbi Phosphatidylinositol [correction] 4,5-bisphosphate signals underlie receptor-specific Gq/11-mediated modulation of N-type Ca2+ channels
    Nikita Gamper
    Department of Physiology, University of Texas Health Science Center at San Antonio, San Antonio, Texas 78229, USA
    J Neurosci 24:10980-92. 2004
    ..We suggest that differential use of PIP2 signals underlies specificity of Gq/11-coupled receptor actions on the channels..
  36. ncbi Desensitization and internalization of metabotropic glutamate receptor 1a following activation of heterologous Gq/11-coupled receptors
    Stuart J Mundell
    Department of Pharmacology, School of Medical Sciences, University of Bristol, Bristol BS8 1TD, UK
    Biochemistry 43:7541-51. 2004
    ..Furthermore, the heterologous desensitization of mGluR1a is dependent upon the splice variant being in an active conformation...
  37. ncbi Activated G alpha q inhibits p110 alpha phosphatidylinositol 3-kinase and Akt
    Lisa M Ballou
    Research Service, Department of Veterans Affairs Medical Center, Northport, New York 11768, USA
    J Biol Chem 278:23472-9. 2003
    ..The inhibitory mechanism seems to be independent of phospholipase C activation and might involve an inhibitory interaction between Galphaq and p110alpha PI3K...
  38. ncbi Protease-activated receptor-2 simultaneously directs beta-arrestin-1-dependent inhibition and Galphaq-dependent activation of phosphatidylinositol 3-kinase
    Ping Wang
    Division of Biomedical Sciences, University of California, Riverside, California 92521, USA
    Biochemistry 45:9374-85. 2006
    ....
  39. ncbi Neurokinin 1 receptors trigger overlapping stimulation and inhibition of CaV2.3 (R-type) calcium channels
    Ulises Meza
    Department of Biology, Utah State University, 5305 Old Main Hill, Logan, UT 84322, USA
    Mol Pharmacol 71:284-93. 2007
    ..This new information concerning R-type calcium channels and NK1 receptors may help in understanding nociception, synaptic plasticity, and other physiological processes...
  40. ncbi Identification of two alpha-subunit species of GTP-binding proteins, Galpha15 and Galphaq, expressed in rat taste buds
    Y Kusakabe
    Department of Applied Biological Chemistry, The University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo 113 8657, Japan
    Biochim Biophys Acta 1403:265-72. 1998
    ..Immunostaining also showed that rat Galpha15, together with the Galphaq, was localized mainly in taste buds. These studies suggest the possibility that these two Galpha proteins function for taste signal transduction in sensory cells...
  41. ncbi Optical biosensor provides insights for bradykinin B(2) receptor signaling in A431 cells
    Ye Fang
    Biochemical Technologies, Science and Technology Division, Corning Incorporated, Sullivan Park, Corning, NY 14831, USA
    FEBS Lett 579:6365-74. 2005
    ..The roles of endocytosis and cytoskeleton modulation in B(2) signaling were also demonstrated...
  42. pmc Regulation of voltage-gated Ca2+ channels by lipids
    Mandy L Roberts-Crowley
    University of Massachusetts Medical School, Worcester, MA 01655, USA
    Cell Calcium 45:589-601. 2009
    ..This unexpectedly simple scheme gives rise to unanticipated predictions and redirects thinking about lipid regulation of VGCCs...
  43. ncbi The heterotrimeric G-protein alpha-subunit Galphaq regulates TCR-mediated immune responses through an Lck-dependent pathway
    Jacob Ngai
    The Biotechnology Centre of Oslo, Nordic EMBL Partnership, University of Oslo, Oslo, Norway
    Eur J Immunol 38:3208-18. 2008
    ..Together these data argue for a role of Galphaq in the fine-tuning of proximal TCR signals at the level of Lck and a negative regulatory role of Galphaq in transcriptional activation of cytokine responses...
  44. ncbi Characterization of the GNAQ promoter and association of increased Gq expression with cardiac hypertrophy in humans
    Ulrich H Frey
    Institute of Pharmacogenetics, D 45122 Essen, Germany
    Eur Heart J 29:888-97. 2008
    ..However, mechanisms regulating transcription of the human GNAQ gene encoding the Gq protein are unknown and single-nucleotide polymorphisms have not been reported...
  45. ncbi Galphaq/11 signaling induces apoptosis through two pathways involving reduction of Akt phosphorylation and activation of RhoA in HeLa cells
    Hiroshi Ueda
    Department of Molecular Neurobiology, Institute for Developmental Research, Aichi Human Service Center, Kamiya cho, Kasugai, 480 0392, Japan
    Exp Cell Res 298:207-17. 2004
    ..These results demonstrate that Gq/11 signaling induces apoptosis by reducing insulin-stimulated Akt phosphorylation through tyrosine dephosphorylation and activating RhoA in HeLa cells...
  46. ncbi Genetic analysis of RGS protein function in Caenorhabditis elegans
    Daniel L Chase
    Department of Molecular Biophysics, The University of North Carolina at Chapel Hill, 27599 7260, USA
    Methods Enzymol 389:305-20. 2004
    ..elegans. This article describes general considerations regarding such experiments and provides detailed protocols for quantitatively measuring G-protein signaling phenotypes in C. elegans...
  47. ncbi G alpha(q/11)-coupled P2Y2 nucleotide receptor inhibits human keratinocyte spreading and migration
    Salma Taboubi
    CISMET, FRE CNRS 2737, Faculte de Pharmacie, Universite d Aix Marseille, France
    FASEB J 21:4047-58. 2007
    ..Collectively, these findings provide evidence for a unique and important role for extracellular nucleotides as efficient autocrine/paracrine regulators of keratinocyte shape and migration during wound healing...
  48. ncbi Two RGS proteins that inhibit Galpha(o) and Galpha(q) signaling in C. elegans neurons require a Gbeta(5)-like subunit for function
    D L Chase
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520, USA
    Curr Biol 11:222-31. 2001
    ..It is unclear how Gbeta(5) might influence RGS activity. In C. elegans there are exactly two GGL-containing RGS proteins, EGL-10 and EAT-16, and they inhibit Galpha(o) and Galpha(q) signaling, respectively...
  49. ncbi Effect of YM-254890, a specific Galphaq/11 inhibitor, on experimental peripheral arterial disease in rats
    Toshio Uemura
    Pharmacology Research Laboratories, Astellas Pharma Inc, 21 Miyukigaoka, Tsukuba, Ibaraki 305 8585, Japan
    Eur J Pharmacol 536:154-61. 2006
    ..These results suggest that the local administration of YM-254890 may be useful for treating peripheral arterial disease...
  50. ncbi Galphaq expression activates EGFR and induces Akt mediated cardiomyocyte survival: dissociation from Galphaq mediated hypertrophy
    Amy L Howes
    Department of Pharmacology, University of California, San Diego, La Jolla, CA 92093 0636, USA
    J Mol Cell Cardiol 40:597-604. 2006
    ..These data demonstrate that increased Galphaq activity can provide anti-apoptotic signals by eliciting EGFR phosphorylation and subsequent Akt activation, independent of the well-known ability of Galphaq signaling to elicit hypertrophy...
  51. ncbi Activation and regulation of platelet-activating factor receptor: role of G(i) and G(q) in receptor-mediated chemotactic, cytotoxic, and cross-regulatory signals
    Stephan L Brown
    Department of Biochemistry, Meharry Medical College, Nashville, TN 37208, USA
    J Immunol 177:3242-9. 2006
    ..Because chemotaxis and cross-desensitization are exclusively mediated by G(i), the data suggest that differential activation of both G(i) and G(q) by PAFR likely mediate specific as well as redundant signaling pathways...
  52. ncbi Galphaq and its Aktions
    David M Harris
    J Mol Cell Cardiol 40:589-92. 2006
  53. pmc Leukemia-associated Rho guanine nucleotide exchange factor promotes G alpha q-coupled activation of RhoA
    Michelle A Booden
    Department of Pharmacology, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, 27599, USA
    Mol Cell Biol 22:4053-61. 2002
    ..Our findings suggest that the RhoA exchange factor LARG, unlike the related p115 RhoGEF and PDZ-RhoGEF proteins, can serve as an effector for Gq-coupled receptors, mediating their functional linkage to RhoA-dependent signaling pathways...
  54. ncbi Effects of G-protein mutations on skin color
    Catherine D Van Raamsdonk
    Department of Genetics, Stanford University School of Medicine, Stanford, California 94305 5323, USA
    Nat Genet 36:961-8. 2004
    ..Our results provide a mechanism that can explain several aspects of human pigmentary variation and show how polymorphism of essential proteins and signaling pathways can affect a single physiologic system...
  55. ncbi Protein kinase C phosphorylates RGS2 and modulates its capacity for negative regulation of Galpha 11 signaling
    M L Cunningham
    Department of Pharmacology, University of North Carolina School of Medicine, Chapel Hill, North Carolina 27599 7365 and the Department of Pharmacology, Emory University School of Medicine, Atlanta, Georgia 30322 3090
    J Biol Chem 276:5438-44. 2001
    ..These results identify for the first time a phosphorylation-induced change in the activity of an RGS protein and suggest a mechanism for potentiation of inositol lipid signaling by PKC...
  56. pmc Activation of G12/G13 results in shape change and Rho/Rho-kinase-mediated myosin light chain phosphorylation in mouse platelets
    B Klages
    Institut fur Pharmakologie, Universitatsklinikum Benjamin Franklin, Freie Universitat Berlin, 14195 Berlin, Germany
    J Cell Biol 144:745-54. 1999
    ..We provide evidence that G12/G13-mediated Rho/Rho-kinase-dependent regulation of MLC phosphorylation participates in receptor-induced platelet shape change...
  57. ncbi Gq signaling is required for allergen-induced pulmonary eosinophilia
    Michael T Borchers
    Division of Pulmonary Medicine, Mayo Clinic, Scottsdale AZ 85259, USA
    J Immunol 168:3543-9. 2002
    ....
  58. ncbi Differential modulation of CaV2.3 Ca2+ channels by Galphaq/11-coupled muscarinic receptors
    R A Bannister
    Department of Biology, Utah State University, Logan, Utah 84322, USA
    Mol Pharmacol 65:381-8. 2004
    ....
  59. pmc Identification of an alternative G{alpha}q-dependent chemokine receptor signal transduction pathway in dendritic cells and granulocytes
    Guixiu Shi
    Trudeau Institute, Saranac Lake, NY 12983, USA
    J Exp Med 204:2705-18. 2007
    ....
  60. ncbi Loss of association between activated Galpha q and Gbetagamma disrupts receptor-dependent and receptor-independent signaling
    Daniel S Evanko
    Department of Microbiology and Immunology, Kimmel Cancer Center, Thomas Jefferson University, 233 S 10th St, 839 BLSB, Philadelphia, PA 19107, USA
    Cell Signal 17:1218-28. 2005
    ..Taken together, results presented here suggest that alpha-betagamma binding is necessary at a point downstream from receptor activation of the heterotrimeric G protein for signal transduction by alpha q...
  61. ncbi Direct voltage control of signaling via P2Y1 and other Galphaq-coupled receptors
    Juan Martinez-Pinna
    Department of Physiology, University of Cambridge, Cambridge CB2 3EG, United Kingdom
    J Biol Chem 280:1490-8. 2005
    ..This effect, which is particularly robust for P2Y1 receptors, has wide-spread implications for cell signaling...
  62. ncbi Activation of nuclear factor {kappa}B by somatostatin type 2 receptor in pancreatic acinar AR42J cells involves G{alpha}14 and multiple signaling components: a mechanism requiring protein kinase C, calmodulin-dependent kinase II, ERK, and c-Src
    Andrew M F Liu
    Department of Biochemistry, Molecular Neuroscience Center, and Biotechnology Research Institute, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China
    J Biol Chem 280:34617-25. 2005
    ..Similar results were also obtained using AR42J cells. These data suggest that activation of the IKK/NFkappaB signaling cascade by SSTR2 requires a complicated network consisting of Galpha(14) and multiple intermediates...
  63. ncbi Signal transducer and activator of transcription 3 activation by the delta-opioid receptor via Galpha14 involves multiple intermediates
    Rico K H Lo
    Department of Biochemistry, Molecular Neuroscience Center, and Biotechnology Research Institute, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China
    Mol Pharmacol 65:1427-39. 2004
    ..These results demonstrate for the first time that Galpha14 activation can lead to STAT3 stimulation via a complex signaling network involving multiple intermediates...
  64. ncbi Stimulation of increases in intracellular calcium and prostaglandin E2 generation in Chinese hamster ovary cells expressing receptor-Galpha16 fusion proteins
    Hinako Suga
    Department of Neurochemistry, Graduate School of Medicine, The University of Tokyo, Hongo, Bunkyo ku, Tokyo 113 0033
    J Biochem 135:605-13. 2004
    ....
  65. ncbi Constitutively active Gq impairs gonadotropin-releasing hormone-induced intracellular signaling and luteinizing hormone secretion in LbetaT2 cells
    Fujun Liu
    Department of Medicine, University of California, San Diego, La Jolla, California 92093, USA
    Mol Endocrinol 19:2074-85. 2005
    ..Taken together, these data demonstrate that prolonged activation of the Gq pathway desensitizes GnRH-induced signaling by selectively down-regulating the PLC-PKC-Ca2+ pathway, leading to reduced LHbeta synthesis and LH secretion...
  66. ncbi Caveolin-1 interacts with 5-HT2A serotonin receptors and profoundly modulates the signaling of selected Galphaq-coupled protein receptors
    Anushree Bhatnagar
    Department of Biochemistry, Case Western Reserve University School of Medicine, 2109 Adelbert Road, Cleveland, OH 44106, USA
    J Biol Chem 279:34614-23. 2004
    ..These studies provide compelling evidence for a prominent role of Cav-1 in regulating the functional activity of not only 5-HT(2A) serotonin receptors but also selected Galpha(q)-coupled receptors...
  67. ncbi Assays of RGS protein modulation by phosphatidylinositides and calmodulin
    Masaru Ishii
    Department of Pharmacology, Osaka University Graduate School of Medicine, Osaka, Japan
    Methods Enzymol 389:105-18. 2004
    ..These assays would be useful for dissecting the physiological modes of action of RGS proteins in controlling G-protein-mediated signaling machinery...
  68. ncbi Replacement of the alpha5 helix of Galpha16 with Galphas-specific sequences enhances promiscuity of Galpha16 toward Gs-coupled receptors
    Anjali Hazari
    Department of Biochemistry, The Biotechnology Research Institute, and the Molecular Neuroscience Center, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China
    Cell Signal 16:51-62. 2004
    ..Similar results were observed for agonist-induced Ca(2+) mobilizations. These results show that incorporating the alpha5 helix of Galpha(s) into Galpha(16) can increase the promiscuity of 16s25 towards G(s)-coupled receptors...
  69. ncbi Estimation of agonist activity at G protein-coupled receptors: analysis of M2 muscarinic receptor signaling through Gi/o,Gs, and G15
    Michael T Griffin
    Department of Physical Sciences, Chapman University, Orange, California, USA
    J Pharmacol Exp Ther 321:1193-207. 2007
    ..Our results show that the RA(i) estimate is a useful measure for quantifying agonist activity across different assay systems and for detecting agonist directed signaling...
  70. ncbi Single-cell imaging of intracellular Ca2+ and phospholipase C activity reveals that RGS 2, 3, and 4 differentially regulate signaling via the Galphaq/11-linked muscarinic M3 receptor
    Stephen C Tovey
    Department of Cell Physiology and Pharmacology, University of Leicester, Leicester, United Kingdom
    Mol Pharmacol 66:1453-64. 2004
    ..Despite little or no effect on responses to maximal receptor activation, RGS4 produced effects on the magnitude, kinetics, and oscillatory behavior of Ca2+ signaling at submaximal levels that were consistent with those of RGS2 and -3...
  71. ncbi G16-mediated activation of nuclear factor kappaB by the adenosine A1 receptor involves c-Src, protein kinase C, and ERK signaling
    Andrew M F Liu
    Department of Biochemistry, Molecular Neuroscience Center, and Biotechnology Research Institute, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China
    J Biol Chem 279:53196-204. 2004
    ..Similar results were obtained using Reh cells. These data suggest that the G(16)-mediated activation of IKK/NFkappaB by CHA required a complex signaling network composed of multiple intermediates...
  72. ncbi Signaling and ligand binding by recombinant neuromedin U receptors: evidence for dual coupling to Galphaq/11 and Galphai and an irreversible ligand-receptor interaction
    Paul J Brighton
    Department of Cell Physiology and Pharmacology, University of Leicester, Leicester, United Kingdom
    Mol Pharmacol 66:1544-56. 2004
    ..Despite structural differences between receptors, there were no apparent differences in their activation, coupling, or regulation...
  73. ncbi PKD in intestinal epithelial cells: rapid activation by phorbol esters, LPA, and angiotensin through PKC
    T Chiu
    Department of Medicine, UCLA School of Medicine and Molecular Biology Institute, Los Angeles, CA 90095, USA
    Am J Physiol Cell Physiol 280:C929-42. 2001
    ..PKD activation was tightly associated with an increase in PKD autophosphorylation at serine 916. Our results identify PKD as a novel early point of convergence and integration of G(i) and G(q) signaling in intestinal epithelial cells...
  74. pmc Alternative splicing of RGS8 gene determines inhibitory function of receptor type-specific Gq signaling
    Osamu Saitoh
    Department of Molecular Cell Signaling, Tokyo Metropolitan Institute for Neuroscience, 2 6 Musashidai, Fuchu shi, Tokyo 183 8526, Japan
    Proc Natl Acad Sci U S A 99:10138-43. 2002
    ....
  75. ncbi Serotonin receptor activation inhibits sodium current and dendritic excitability in prefrontal cortex via a protein kinase C-dependent mechanism
    David B Carr
    Department of Physiology, Northwestern University, Chicago, Illinois 60611, USA
    J Neurosci 22:6846-55. 2002
    ..These findings show that 5-HT2a,c receptor activation reduces dendritic excitability and may negatively modulate activity-dependent dendritic synaptic plasticity...
  76. ncbi Regulation of GTP-binding protein alpha q (Galpha q) signaling by the ezrin-radixin-moesin-binding phosphoprotein-50 (EBP50)
    Moulay Driss Rochdi
    Service de Rhumatologie, Faculté de Médecine and Centre de Recherche Clinique CHUS, Universite de Sherbrooke, Sherbrooke, Quebec J1H 5N4, Canada
    J Biol Chem 277:40751-9. 2002
    ..This study constitutes the first demonstration of an interaction between a G protein alpha subunit and another protein through a PDZ domain, with broad implications in the regulation of diverse physiological systems...
  77. ncbi Genetic and pharmacological dissection of pathways involved in the angiotensin II-mediated depression of baroreflex function
    Liang Fong Wong
    University Research Centre for Neuroendocrinology, University of Bristol, Bristol Royal Infirmary, Bristol BS2 8HW, UK
    FASEB J 16:1595-601. 2002
    ..In contrast, multiple site disruption of a pathway leading to eNOS activation via the serine/threonine kinase Akt was ineffective..
  78. pmc Orphan G protein-coupled receptors MrgA1 and MrgC11 are distinctively activated by RF-amide-related peptides through the Galpha q/11 pathway
    Sang Kyou Han
    Division of Biology 147 75, California Institute of Technology, Pasadena 91125, USA
    Proc Natl Acad Sci U S A 99:14740-5. 2002
    ..The differences in ligand specificity also suggest functional diversity amongst the Mrg receptors...
  79. ncbi Galpha(12/13) mediates alpha(1)-adrenergic receptor-induced cardiac hypertrophy
    Yoshiko Maruyama
    Laboratory of Pharmacology and Toxicology, Graduate School of Pharmaceutical Sciences, University of Tokyo, Tokyo, Japan
    Circ Res 91:961-9. 2002
    ....
  80. ncbi Galphaq signaling is required for Rho-dependent transcriptional activation of the cyclooxygenase-2 promoter in fibroblasts
    Lee W Slice
    Division of Digestive Diseases, Department of Medicine, CURE Digestive Diseases Research Center, Greater Los Angeles VA Medical Center, University of California, Los Angeles, California 90095, USA
    J Cell Physiol 194:127-38. 2003
    ..These results demonstrate that calcium signaling mediated by Galpha(q) and Rho play critical roles in GRP-dependent COX-2 expression in fibroblasts...
  81. ncbi Involvement of G(q/11) in signal transduction in the mammalian vomeronasal organ
    Kennedy S Wekesa
    Alabama State University, Biomedical Research and Training Programs, Montgomery, AL 36104 0271, USA
    J Exp Biol 206:827-32. 2003
    ..Our observations indicate that pheromones in male urine act on vomeronasal neurons in the female VNO via a receptor-mediated, G alpha(q/11)-protein-dependent increase in IP(3) levels...
  82. ncbi Regulation of phospholipase C-beta activity by phosphatidic acid: isoform dependence, role of protein kinase C, and G protein subunits
    Irene Litosch
    Department of Molecular and Cellular Pharmacology, University of Miami School of Medicine, Miami, Florida 33101, USA
    Biochemistry 42:1618-23. 2003
    ..PA may be an important component of a receptor mediated signaling mechanism that determines PLC-beta(1) activation...
  83. ncbi A functional enhanced green fluorescent protein (EGFP)-tagged angiotensin II at(1a) receptor recruits the endogenous Galphaq/11 protein to the membrane and induces its specific internalization independently of receptor-g protein coupling in HEK-293 cells
    S Miserey-Lenkei
    , , 3 rue d'Ulm, 75005 Paris, France
    Mol Endocrinol 15:294-307. 2001
    ....
  84. ncbi Co-expressions of different opioid receptor types differentially modulate their signaling via G(16)
    Maurice K C Ho
    Department of Biochemistry, Molecular Neuroscience Center and Biotechnology Research Institute, Hong Kong University of Science and Technology, Hong Kong, China
    Neurosignals 11:115-22. 2002
    ..It is suggested that the co-expressions of two different opioid receptor types indeed modify their downstream signaling events...
  85. pmc Dual actions of the Galpha(q) agonist Pasteurella multocida toxin to promote cardiomyocyte hypertrophy and enhance apoptosis susceptibility
    Abdelkarim Sabri
    Department of Pharmacology, College of Physicians and Surgeons, Columbia University, New York, NY 10032, USA
    Circ Res 90:850-7. 2002
    ..Because inhibition of Akt enhances cardiomyocyte susceptibility to apoptosis, this pathway is predicted to contribute to the transition from hypertrophy to cardiac decompensation and could be targeted for therapy in heart failure...
  86. ncbi The Ca2+-sensing receptor activates cytosolic phospholipase A2 via a Gqalpha -dependent ERK-independent pathway
    M E Handlogten
    Division of Nephrology, Department of Medicine, University of Florida, Gainesville, Florida 32610, USA
    J Biol Chem 276:13941-8. 2001
    ..These results demonstrate that the CaR activates cPLA(2) via a Galpha(q), PLC, Ca(2+)-CaM, and calmodulin-dependent protein kinase-dependent pathway that is independent the ERK pathway...
  87. ncbi Tyrosine kinase activation by the angiotensin II receptor in the absence of calcium signaling
    T N Doan
    Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, Georgia 30322, USA
    J Biol Chem 276:20954-8. 2001
    ..These studies are the first to show the ability of a seven-transmembrane receptor to activate intracellular tyrosine kinase pathways in the absence of a G-protein-coupled rise in intracellular calcium...
  88. ncbi Galphaq-coupled receptor internalization specifically induced by Galphaq signaling. Regulation by EBP50
    Moulay Driss Rochdi
    Service de Rhumatologie, Faculté de Médecine and Centre de Recherche Clinique, Universite de Sherbrooke, Sherbrooke, Quebec J1H 5N4, Canada
    J Biol Chem 278:17827-37. 2003
    ..Furthermore, a new function has been described for EBP50 in its capacity to inhibit receptor endocytosis...
  89. ncbi Measuring RGS protein interactions with Gq alpha
    Peter Chidiac
    Department of Pharmacology and Toxicology, Faculty of Medicine and Dentistry, University of Western Ontario, London, Ontario N6A 5C1, Canada
    Methods Enzymol 344:686-702. 2002
  90. ncbi Phosphatidylinositol 4,5-bisphosphate modifies tubulin participation in phospholipase Cbeta1 signaling
    Juliana S Popova
    Department of Physiology and Biophysics, University of Illinois at Chicago, College of Medicine, Chicago, Illinois 60612 7342, USA
    J Neurosci 22:1668-78. 2002
    ..Rapid changes in membrane lipid composition or in the cytoskeleton might modify neuronal signaling through such a mechanism...
  91. ncbi The C domain of netrin UNC-6 silences calcium/calmodulin-dependent protein kinase- and diacylglycerol-dependent axon branching in Caenorhabditis elegans
    Qun Wang
    Department of Pathology, Robert Wood Johnson Medical School, Piscataway, New Jersey 08854 5635, USA
    J Neurosci 22:2274-82. 2002
    ....
  92. ncbi Inositol 1,4,5-trisphosphate transduction cascade in taste reception of the fleshfly, Boettcherisca peregrina
    Masayuki Koganezawa
    Biological Institute, Graduate School of Science, Tohoku University, Aoba ku, Sendai 980 8578, Japan
    J Neurobiol 51:66-83. 2002
    ..These results suggest that the IP3 transduction cascade is involved in the response of the sugar receptor cell of the fly...
  93. ncbi Calcium-sensing receptor-mediated activation of phospholipase C-gamma1 is downstream of phospholipase C-beta and protein kinase C in MC3T3-E1 osteoblasts
    S L Godwin
    Department of Orthodontics, University of Connecticut Health Center, Farmington, CT 06030, USA
    Bone 30:559-66. 2002
    ....
  94. ncbi Stimulation of 5-HT(2) receptors in prefrontal pyramidal neurons inhibits Ca(v)1.2 L type Ca(2+) currents via a PLCbeta/IP3/calcineurin signaling cascade
    Michelle Day
    Department of Physiology Northwestern University Institute for Neuroscience, Northwestern University Medical School, Chicago, Illinois 60611, USA
    J Neurophysiol 87:2490-504. 2002
    ..2 L-type Ca(2+) currents. This modulation and its blockade by atypical neuroleptics could have wide-ranging effects on synaptic integration and long-term gene expression in deep-layer prefrontal pyramidal neurons...
  95. ncbi Signaling mechanism for receptor-activated canonical transient receptor potential 3 (TRPC3) channels
    Mohamed Trebak
    Laboratory of Signal Transduction, NIEHS National Institutes of Health, Research Triangle Park, NC 27709, USA
    J Biol Chem 278:16244-52. 2003
    ..We conclude that receptor-mediated activation of phospholipase C in intact cells activates TRPC3 via diacylglycerol production, independently of G proteins, protein kinase C, or inositol 1,4,5-trisphosphate...
  96. ncbi Gonadotropin-releasing hormone-desensitized LbetaT2 gonadotrope cells are refractory to acute protein kinase C, cyclic AMP, and calcium-dependent signaling
    Fujun Liu
    Department of Medicine, University of California San Diego Cancer Center, San Diego, California 92093, USA
    Endocrinology 144:4354-65. 2003
    ..Sustained GnRH also desensitized PKC signaling by down-regulating the delta, epsilon, and theta isoforms of PKC. We further show that chronic GnRH treatment causes heterologous desensitization of other Gq-coupled receptors...
  97. ncbi Rapid signaling of estrogen in hypothalamic neurons involves a novel G-protein-coupled estrogen receptor that activates protein kinase C
    Jian Qiu
    Department of Physiology and Pharmacology, Oregon Health and Science University, Portland, Oregon 97239 3098, USA
    J Neurosci 23:9529-40. 2003
    ..Therefore, this unique Gq-coupled estrogen receptor may be involved in rapid signaling in hypothalamic neurons that are critical for normal homeostatic functions...
  98. ncbi G beta 5.RGS7 inhibits G alpha q-mediated signaling via a direct protein-protein interaction
    D Scott Witherow
    Department of Molecular and Cellular Pharmacology and the Neuroscience Program, University of Miami School of Medicine, Miami, Florida 33136, USA
    J Biol Chem 278:21307-13. 2003
    ..Gbeta5 complex and cyan fluorescence protein-tagged Galphaq, indicating a direct interaction between these molecules...
  99. ncbi Structure-function studies of the C3a-receptor: C-terminal serine and threonine residues which influence receptor internalization and signaling
    Britta Settmacher
    Department of Medical Microbiology, Medical School Hannover, Hannover, Germany
    Eur J Immunol 33:920-7. 2003
    ....
  100. ncbi Chronic fluoxetine differentially affects 5-hydroxytryptamine (2A) receptor signaling in frontal cortex, oxytocin- and corticotropin-releasing factor-containing neurons in rat paraventricular nucleus
    K J Damjanoska
    Department of Pharmacology, Loyola University Chicago, Stritch School of Medicine, Maywood, IL 60153, USA
    J Pharmacol Exp Ther 306:563-71. 2003
    ....
  101. ncbi Assay for G protein-dependent activation of phospholipase C beta using purified protein components
    Mousumi Ghosh
    Department of Pharmacology and Physiology, University of Rochester School of Medicine, NY, USA
    Methods Mol Biol 237:67-75. 2004
    ..It can also be used to assess the functionality of the components after modification by mutagenesis, chemical modification, or in the presence of competing molecules...

Research Grants23

  1. Functional analysis of KSHV GPCR in human lymphocytes
    Mark Cannon; Fiscal Year: 2007
    ..Dr. Cannon will be studying at the Weill Medical College and School of Medical Sciences of Cornell University and working in the lab under the mentorship of Dr. Ethel Cesarman of the Department of Pathology. ..
  2. ELECTRIC STUDIES OF EXCITATION, SECRETION & CONTRACTION
    Bertil Hille; Fiscal Year: 2007
    ..What are the Ca 2+ buffering and flux properties of these organelles? Our work in these cells concerns processes whose failure leads to disease and whose modulation offers new therapies. ..
  3. Alpha-Synuclein Conditional Knock-in Mice as Novel Models of Parkinson's Disease
    Darren Moore; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  4. Orexinergic Pathways in Central Autonomic Control
    WILLIS SAMSON; Fiscal Year: 2005
    ..abstract_text> ..
  5. The International Symposium on Regulatory Peptides
    JUAN ROZENGURT; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  6. NUCLEOTIDE-MEDIATED LEUKOCYTE STIMULATION
    Satya Kunapuli; Fiscal Year: 2004
    ..The studies proposed in this application will also help us understand the mechanisms of neutrophil activation and contribute to the knowledge of better treatments and to the design of therapeutics for inflammatory diseases. ..
  7. Control of Meiotic Homolog Segregation in Arabidopsis
    Hong Ma; Fiscal Year: 2004
    ..Furthermore, molecular and cell biological experiments will be done to characterize the expression pattern and protein localization of SDS, and to isolate a putative SDS-like gene. ..
  8. Functional G Protein pathways in platelet activation
    Satya P Kunapuli; Fiscal Year: 2010
    ..These studies will enhance our understanding of the signaling pathways and their role in platelet activation, and might identify potential newer targets for the treatment of thrombosis. ..
  9. PHYSIOLOGY OF THE PROLACTIN RELEASING PEPTIDES
    Willis K Samson; Fiscal Year: 2010
    ..Additionally, these studies will provide further insight into the coordinated hormonal and autonomic responses to stress. ..
  10. Mitogen activated protein kinases regulation-PTH/PTHrP
    Hesham Tawfeek; Fiscal Year: 2008
    ..Primary osteoblastic cell culture from wild type and genetically engineered mice as well as several other biochemical and pharmacological tools will be employed to achieve the goals of the study. ..
  11. Platelet ADP receptors
    Satya Kunapuli; Fiscal Year: 2008
    ..We further propose to evaluate the relative contribution of the P2Y1 and the P2Y12 receptors to thrombus formation, using P2Y1 or P2Y12 deficient mice and treatment with antagonists and models of in vivo thrombosis and thromboembolism. ..
  12. Pasteurella multocida toxin: Structure and Activity
    Brenda Wilson; Fiscal Year: 2008
    ..4) To characterize the cellular receptor(s) and to elucidate the internalization pathway(s) utilized by PMT to gain entry into cells. ..
  13. GI PEPTIDE SIGNALING THROUGH TYROSINE PHOSPHORYLATION
    JUAN ENRIQUE ROZENGURT; Fiscal Year: 2010
    ....
  14. Rho-Dependent COX-2 Expression in Intestinal Cells
    Lee Slice; Fiscal Year: 2007
    ..3) Define the role of Rho and of Protein Kinases acting upstream and downstream of Rho in COX-2 expression in intestinal epithelial cells. 4) Characterize COX-2 promoter elements that are responsive to Rho. ..
  15. Leak potassium channels in striatal cholinergic neurons
    ALLISON BERG; Fiscal Year: 2006
    ..Finally, I confirm my findings using a mouse model in which TASK-3 is conditionally deleted (Specific Aim 3). ..
  16. GENETICS OF FAMILIAL FOCAL SEGMENTAL GLOMERULOSCLEROSIS
    Michelle Winn; Fiscal Year: 2005
    ..New mutations found in the genes known to cause FSGS will enhance our understanding of the genetics and biology of thi., disorder, takin 9 advantage of our entire resource of families. ..
  17. BRAIN GALANIN RECEPTORS AND EPILEPSY
    Andrey Mazarati; Fiscal Year: 2006
    ..abstract_text> ..
  18. RAD51 gene functions in mitosis and meiosis of zebrafish
    Hong Ma; Fiscal Year: 2004
    ..We will test this hypothesis using morpholino knockdowns of these genes. These experiments will pave the way for more extensive studies in the near future. ..
  19. AUTOSOMAL DOMINANT FOCAL SEGMENTAL GLOMERULOSCLEROSIS
    Michelle Winn; Fiscal Year: 2004
    ..Information derived from this research will also allow a better understanding of the pathogenesis of nonhereditary forms of FSGS and may provide insights regarding management of the more common idiopathic forms of this disorder. ..
  20. CATECHOLS AND ADENOSINE IN MYOCARDIAL PRECONDITIONING
    Michael Cohen; Fiscal Year: 2006
    ..Finally, I will see if NECA's salvage of myocardium at reperfusion translates into rapid functional benefits. It is hoped that the potential of this new pharmacologic approach can be defined. ..
  21. SYNAPTIC PLASTICITY OF HYPOTHALAMIC NEURONS--ESTROGENS
    OLINE RONNEKLEIV; Fiscal Year: 2002
    ....
  22. MYOGENIC DISORDERS OF THE GALLBLADDER
    Jose Behar; Fiscal Year: 2004
    ..The results of these studies may provide evidence and a rationale in support for using hydrophobic bile acids in the prophylactic treatment of this complication. ..