gtp binding protein alpha subunits

Summary

Summary: The GTPase-containing subunits of heterotrimeric GTP-binding proteins. When dissociated from the heterotrimeric complex these subunits interact with a variety of second messenger systems. Hydrolysis of GTP by the inherent GTPase activity of the subunit causes it to revert to its inactive (heterotrimeric) form. The GTP-Binding protein alpha subunits are grouped into families according to the type of action they have on second messenger systems.

Top Publications

  1. pmc Different signaling and cell death roles of heterotrimeric G protein alpha and beta subunits in the Arabidopsis oxidative stress response to ozone
    Junghee H Joo
    Huck Institutes of the Life Sciences and Biology Department, Pen State University, University Park, Pensylvania 16802, USA
    Plant Cell 17:957-70. 2005
  2. pmc Heterotrimeric G proteins facilitate Arabidopsis resistance to necrotrophic pathogens and are involved in jasmonate signaling
    Yuri Trusov
    Plant Genetic Engineering Laboratory, Department of Botany, School of Integrative Biology, University of Queensland, Brisbane, Queensland 4072, Australia
    Plant Physiol 140:210-20. 2006
  3. pmc G-protein complex mutants are hypersensitive to abscisic acid regulation of germination and postgermination development
    Sona Pandey
    Department of Biology, Pennsylvania State University, University Park, Pennsylvania 16802 5301, USA
    Plant Physiol 141:243-56. 2006
  4. ncbi Modulation of cell proliferation by heterotrimeric G protein in Arabidopsis
    H Ullah
    Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    Science 292:2066-9. 2001
  5. ncbi A seven-transmembrane RGS protein that modulates plant cell proliferation
    Jin Gui Chen
    Department of Biology, The University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 3280, USA
    Science 301:1728-31. 2003
  6. ncbi A Saccharomyces cerevisiae G-protein coupled receptor, Gpr1, is specifically required for glucose activation of the cAMP pathway during the transition to growth on glucose
    L Kraakman
    Laboratorium voor Moleculaire Celbiologie, Katholieke Universiteit Leuven, Institute of Botany and Microbiology
    Mol Microbiol 32:1002-12. 1999
  7. pmc The beta-subunit of the Arabidopsis G protein negatively regulates auxin-induced cell division and affects multiple developmental processes
    Hemayet Ullah
    Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 3280, USA
    Plant Cell 15:393-409. 2003
  8. pmc Differential roles of Arabidopsis heterotrimeric G-protein subunits in modulating cell division in roots
    Jin Gui Chen
    Department of Botany, University of British Columbia, Vancouver, British Columbia V6T 1Z4, Canada
    Plant Physiol 141:887-97. 2006
  9. ncbi Novel sensing mechanisms and targets for the cAMP-protein kinase A pathway in the yeast Saccharomyces cerevisiae
    J M Thevelein
    Laboratorium voor Moleculaire Celbiologie, Katholieke Universiteit Leuven, Kardinaal Mercierlaan 92, B 3001 Leuven Heverlee, Flanders, Belgium
    Mol Microbiol 33:904-18. 1999
  10. ncbi Regulators of G-protein signalling in Aspergillus nidulans: RgsA downregulates stress response and stimulates asexual sporulation through attenuation of GanB (Galpha) signalling
    Kap Hoon Han
    Department of Food Microbiology and Toxicology and Food Research Institute, University of Wisconsin, Madison, WI 53706, USA
    Mol Microbiol 53:529-40. 2004

Detail Information

Publications189 found, 100 shown here

  1. pmc Different signaling and cell death roles of heterotrimeric G protein alpha and beta subunits in the Arabidopsis oxidative stress response to ozone
    Junghee H Joo
    Huck Institutes of the Life Sciences and Biology Department, Pen State University, University Park, Pensylvania 16802, USA
    Plant Cell 17:957-70. 2005
    ....
  2. pmc Heterotrimeric G proteins facilitate Arabidopsis resistance to necrotrophic pathogens and are involved in jasmonate signaling
    Yuri Trusov
    Plant Genetic Engineering Laboratory, Department of Botany, School of Integrative Biology, University of Queensland, Brisbane, Queensland 4072, Australia
    Plant Physiol 140:210-20. 2006
    ..We hypothesize that Gbetagamma acts as a direct or indirect enhancer of the jasmonate signaling pathway in plants...
  3. pmc G-protein complex mutants are hypersensitive to abscisic acid regulation of germination and postgermination development
    Sona Pandey
    Department of Biology, Pennsylvania State University, University Park, Pennsylvania 16802 5301, USA
    Plant Physiol 141:243-56. 2006
    ..Thus, cell- and tissue-specific functional interaction in response to a given signal such as ABA may determine the distinct pathways regulated by the individual members of the G-protein complex...
  4. ncbi Modulation of cell proliferation by heterotrimeric G protein in Arabidopsis
    H Ullah
    Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    Science 292:2066-9. 2001
    ..Results from loss of function and ectopic expression and activation of GPA1 indicate that this subunit is a positive modulator of cell division in plants...
  5. ncbi A seven-transmembrane RGS protein that modulates plant cell proliferation
    Jin Gui Chen
    Department of Biology, The University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 3280, USA
    Science 301:1728-31. 2003
    ..These findings suggest that AtRGS1 is a critical modulator of plant cell proliferation...
  6. ncbi A Saccharomyces cerevisiae G-protein coupled receptor, Gpr1, is specifically required for glucose activation of the cAMP pathway during the transition to growth on glucose
    L Kraakman
    Laboratorium voor Moleculaire Celbiologie, Katholieke Universiteit Leuven, Institute of Botany and Microbiology
    Mol Microbiol 32:1002-12. 1999
    ..This appears to be the first example of a GPCR system activated by a nutrient in eukaryotic cells. Hence, a subfamily of GPCRs might be involved in nutrient sensing...
  7. pmc The beta-subunit of the Arabidopsis G protein negatively regulates auxin-induced cell division and affects multiple developmental processes
    Hemayet Ullah
    Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 3280, USA
    Plant Cell 15:393-409. 2003
    ..These results suggest that although auxin-regulated cell division is not coupled directly by a G protein, the Gbeta-subunit attenuates this auxin pathway upstream of the control of mRNA steady state levels...
  8. pmc Differential roles of Arabidopsis heterotrimeric G-protein subunits in modulating cell division in roots
    Jin Gui Chen
    Department of Botany, University of British Columbia, Vancouver, British Columbia V6T 1Z4, Canada
    Plant Physiol 141:887-97. 2006
    ..These results suggest that Arabidopsis heterotrimeric G-protein subunits have differential and opposing roles in the modulation of cell division in roots...
  9. ncbi Novel sensing mechanisms and targets for the cAMP-protein kinase A pathway in the yeast Saccharomyces cerevisiae
    J M Thevelein
    Laboratorium voor Moleculaire Celbiologie, Katholieke Universiteit Leuven, Kardinaal Mercierlaan 92, B 3001 Leuven Heverlee, Flanders, Belgium
    Mol Microbiol 33:904-18. 1999
    ..Cln3 appears to play a crucial role in the connection between the availability of certain nutrients and Cdc28 kinase activity, but it remains to be clarified which nutrient-controlled pathways control Cln3 levels...
  10. ncbi Regulators of G-protein signalling in Aspergillus nidulans: RgsA downregulates stress response and stimulates asexual sporulation through attenuation of GanB (Galpha) signalling
    Kap Hoon Han
    Department of Food Microbiology and Toxicology and Food Research Institute, University of Wisconsin, Madison, WI 53706, USA
    Mol Microbiol 53:529-40. 2004
    ..Our findings define a second and specific RGS-Galpha pair in A. nidulans, which may govern upstream regulation of fungal cellular responses to environmental changes...
  11. ncbi Activation of the phosphatidylinositol 3-kinase Vps34 by a G protein alpha subunit at the endosome
    Janna E Slessareva
    Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    Cell 126:191-203. 2006
    ..These findings reveal two new components of the pheromone signaling pathway. More remarkably, these proteins appear to comprise a preformed effector-G beta subunit assembly and function at the endosome rather than at the plasma membrane...
  12. ncbi Asymmetrically distributed C. elegans homologs of AGS3/PINS control spindle position in the early embryo
    Monica Gotta
    Wellcome Cancer Research United Kingdom Institute, Tennis Court Road, University of Cambridge, United Kingdom
    Curr Biol 13:1029-37. 2003
    ..The Galpha subunits act downstream of the PAR polarity proteins, which control the relative pulling forces acting on the poles. How these heterotrimeric G proteins are regulated and how they control spindle position is still unknown...
  13. ncbi Plant G protein heterotrimers require dual lipidation motifs of Galpha and Ggamma and do not dissociate upon activation
    Merel J W Adjobo-Hermans
    Swammerdam Institute for Life Sciences, Section of Molecular Cytology, Centre for Advanced Microscopy, University of Amsterdam, Kruislaan 316, 1098 SM, Amsterdam, The Netherlands
    J Cell Sci 119:5087-97. 2006
    ..Importantly, rendering GPalpha1 constitutively active did not cause a FRET decrease in the heterotrimer, suggesting no dissociation upon GPalpha1 activation...
  14. ncbi Activation state of the Ras2 protein and glucose-induced signaling in Saccharomyces cerevisiae
    Sonia Colombo
    Dipartimento di Biotecnologie e Bioscienze, Universita Milano Bicocca, Piazza della Scienza 2, 20126 Milano, Italy
    J Biol Chem 279:46715-22. 2004
    ..Strains with reduced feedback inhibition on cAMP synthesis also display elevated basal and induced Ras2 GTP loading consistent with the Ras2 protein acting as a target of the feedback-inhibition mechanism...
  15. pmc Genetic interactions between polymorphisms that affect gene expression in yeast
    Rachel B Brem
    Program in Computational Biology, Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue N, M2 B876, Seattle, Washington 98109, USA
    Nature 436:701-3. 2005
    ..Our results indicate that genetic interactions are widespread in the genetics of transcript levels, and that many QTLs will be missed by single-locus tests but can be detected by two-stage tests that allow for interactions...
  16. pmc GTPase acceleration as the rate-limiting step in Arabidopsis G protein-coupled sugar signaling
    Christopher A Johnston
    Department of Pharmacology, Lineberger Comprehensive Cancer Center and Neuroscience Center, University of North Carolina, Chapel Hill, NC 27599 7365, USA
    Proc Natl Acad Sci U S A 104:17317-22. 2007
    ....
  17. pmc The GanB Galpha-protein negatively regulates asexual sporulation and plays a positive role in conidial germination in Aspergillus nidulans
    Mi Hee Chang
    Division of Biological Sciences, Chonbuk National University, Chonju 561 756, Republic of Korea
    Genetics 167:1305-15. 2004
    ..Taken together, these results indicated that GanB plays a positive role during germination, possibly through carbon source sensing, and negatively regulates asexual conidiation in A. nidulans...
  18. pmc Subcellular localization of LGN during mitosis: evidence for its cortical localization in mitotic cell culture systems and its requirement for normal cell cycle progression
    Rachna Kaushik
    Institute of Molecular and Cell Biology, Singapore 117609
    Mol Biol Cell 14:3144-55. 2003
    ..The results also show that the cortical localization of LGN is dependent on microfilaments and that interfering with LGN function in cultured cell lines causes early disruption to cell cycle progression...
  19. ncbi Downregulation of cone-specific gene expression and degeneration of cone photoreceptors in the Rpe65-/- mouse at early ages
    Sergey L Znoiko
    Departments of Ophthalmology, Medical University of South Carolina, Charleston, South Carolina, USA
    Invest Ophthalmol Vis Sci 46:1473-9. 2005
    ..Rod photoreceptors in the RPE65-knockout (Rpe65(-/-)) mouse are known to degenerate slowly with age. This study was designed to examine cone photoreceptors and the expression of cone-specific genes in the Rpe65(-/-) mouse...
  20. ncbi Mammalian Pins is a conformational switch that links NuMA to heterotrimeric G proteins
    Quansheng Du
    Center for Cell Signaling, Department of Microbiology, University of Virginia School of Medicine, Charlottesville, VA 22908, USA
    Cell 119:503-16. 2004
    ..We propose that a related switch mechanism might operate in asymmetric cell divisions in the fly and nematode...
  21. pmc A point mutation to Galphai selectively blocks GoLoco motif binding: direct evidence for Galpha.GoLoco complexes in mitotic spindle dynamics
    Francis S Willard
    Department of Pharmacology, Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    J Biol Chem 283:36698-710. 2008
    ..GoLoco motif protein complexes in microtubule dynamics and spindle function during cell division as well as to delineate potential roles for GoLoco motifs in receptor-mediated signal transduction...
  22. pmc The Arabidopsis putative G protein-coupled receptor GCR1 interacts with the G protein alpha subunit GPA1 and regulates abscisic acid signaling
    Sona Pandey
    Biology Department, Pen State University, University Park, Pensylvania, 16802, USA
    Plant Cell 16:1616-32. 2004
    ..Because gpa1 mutants exhibit insensitivity in aspects of guard cell ABA and S1P responses, whereas gcr1 mutants exhibit hypersensitivity, GCR1 may act as a negative regulator of GPA1-mediated ABA responses in guard cells...
  23. ncbi G protein regulation of ion channels and abscisic acid signaling in Arabidopsis guard cells
    X Q Wang
    Biology Department, Pennsylvania State University, 208 Mueller Laboratory, University Park, PA 16802 5301, USA
    Science 292:2070-2. 2001
    ..Manipulation of G protein status in guard cells may provide a mechanism for controlling plant water balance...
  24. pmc GPR1 encodes a putative G protein-coupled receptor that associates with the Gpa2p Galpha subunit and functions in a Ras-independent pathway
    Y Xue
    Department of Cell Biology and Anatomy, Mount Sinai School of Medicine, New York, NY 10029, USA
    EMBO J 17:1996-2007. 1998
    ....
  25. pmc Dynamic localization of LIN-5 and GPR-1/2 to cortical force generation domains during spindle positioning
    Dae Hwi Park
    Section of Molecular and Cellular Biology, University of California, Davis, CA 95616, USA
    Dev Biol 315:42-54. 2008
    ....
  26. pmc A novel regulator of G protein signalling in yeast, Rgs2, downregulates glucose-activation of the cAMP pathway through direct inhibition of Gpa2
    M Versele
    Laboratorium voor Moleculaire Celbiologie, Instituut voor Plantkunde en Microbiologie, Katholieke Universiteit Leuven, Kardinaal Mercierlaan 92, B 3001 Leuven Heverlee, Flanders, Belgium
    EMBO J 18:5577-91. 1999
    ..Moreover, Rgs2 and Sst2 exert specific, non-overlapping functions, and deletion mutants in Rgs2 and Sst2 are complemented to some extent by different mammalian RGS proteins...
  27. ncbi Genetic characterization reveals no role for the reported ABA receptor, GCR2, in ABA control of seed germination and early seedling development in Arabidopsis
    Yajun Gao
    Department of Botany, University of British Columbia, Vancouver, BC V6T 1Z4 Canada
    Plant J 52:1001-13. 2007
    ..Taken together, our results do not support the notion that GCR2 is an ABA-signaling GPCR in seed germination and early seedling development...
  28. pmc Expansion of signal transduction by G proteins. The second 15 years or so: from 3 to 16 alpha subunits plus betagamma dimers
    Lutz Birnbaumer
    Laboratory of Signal Transduction, National Institute of Environmental Health Sciences, NIH, DHHS, Research Triangle Park, NC 27709, USA
    Biochim Biophys Acta 1768:772-93. 2007
    ..Emphasis is placed in presenting how the field developed with the hope of conveying why many of the new findings were made...
  29. pmc Signal transduction by Tga3, a novel G protein alpha subunit of Trichoderma atroviride
    Susanne Zeilinger
    Research Area of Gene Technology and Applied Biochemistry, Institute for Chemical Engineering, Vienna University of Technology, Getreidemarkt 9, A 1060 Vienna, Austria
    Appl Environ Microbiol 71:1591-7. 2005
    ..Thus, T. atroviride Tga3 has a general role in vegetative growth and can alter mycoparasitism-related characteristics, such as infection structure formation and chitinase gene expression...
  30. ncbi G-protein-coupled receptor signaling components localize in both sarcolemmal and intracellular caveolin-3-associated microdomains in adult cardiac myocytes
    Brian P Head
    Department of Pharmacology, University of California, San Diego, La Jolla, California 92093 0636, USA
    J Biol Chem 280:31036-44. 2005
    ....
  31. pmc G protein signaling governing cell fate decisions involves opposing Galpha subunits in Cryptococcus neoformans
    Yen Ping Hsueh
    Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, NC 27710, USA
    Mol Biol Cell 18:3237-49. 2007
    ..The incorporation of an additional Galpha into the regulatory circuit enabled increased signaling complexity and facilitated cell fate decisions involving choice between yeast growth and filamentous asexual/sexual development...
  32. pmc Sst2, a negative regulator of pheromone signaling in the yeast Saccharomyces cerevisiae: expression, localization, and genetic interaction and physical association with Gpa1 (the G-protein alpha subunit)
    H G Dohlman
    Department of Pharmacology, Boyer Center for Molecular Medicine, Yale University School of Medicine, New Haven, Connecticut 06536 0812, USA
    Mol Cell Biol 16:5194-209. 1996
    ..These results demonstrate that Sst2 and Gpa1 interact physically and suggest that Sst2 is a direct negative regulator of Gpa1...
  33. pmc Glucose monitoring in fission yeast via the Gpa2 galpha, the git5 Gbeta and the git3 putative glucose receptor
    R M Welton
    Department of Biology, Boston College, Chestnut Hill, Massachusetts 02467, USA
    Genetics 156:513-21. 2000
    ..Since the git3 deletion is fully suppressed by the gpa2(R176H) allele with respect to fbp1 transcription, git3 appears to encode a G protein-coupled glucose receptor responsible for adenylate cyclase activation in S. pombe...
  34. pmc Heterotrimeric G-protein subunit function in Candida albicans: both the alpha and beta subunits of the pheromone response G protein are required for mating
    Daniel Dignard
    NRC Biotechnology Research Institute, 6100 Royalmount Ave, Montreal, Quebec, Canada H4P 2R2
    Eukaryot Cell 7:1591-9. 2008
    ....
  35. ncbi The RGSZ2 protein exists in a complex with mu-opioid receptors and regulates the desensitizing capacity of Gz proteins
    Javier Garzón
    Neurofarmacologia, Instituto de Neurobiologia Santiago Ramon y Cajal, CSIC, Madrid, Spain
    Neuropsychopharmacology 30:1632-48. 2005
    ..These results indicate that both RGSZ1 and RGSZ2 proteins influence mu receptor signaling by sequestering Galpha subunits, therefore behaving as effector antagonists...
  36. pmc Whole proteome identification of plant candidate G-protein coupled receptors in Arabidopsis, rice, and poplar: computational prediction and in-vivo protein coupling
    Timothy E Gookin
    Department of Biology, The Pennsylvania State University, Mueller Laboratory, University Park, PA 16802, USA
    Genome Biol 9:R120. 2008
    ....
  37. ncbi RGS17/RGSZ2, a novel regulator of Gi/o, Gz, and Gq signaling
    Helen Mao
    Department of Neuroscience, Ottawa Health Research Institute, University of Ottawa, Ottawa, Ontario K1H 8M5, Canada
    J Biol Chem 279:26314-22. 2004
    ..Differences observed between in vitro GAP assays and whole-cell signaling suggest additional determinants of the G-protein specificity of RGS GAP effects that could include receptors and effectors...
  38. ncbi The G protein alpha subunit Tga1 of Trichoderma atroviride is involved in chitinase formation and differential production of antifungal metabolites
    Barbara Reithner
    Research Area Gene Technology and Applied Biochemistry, Working Group Molecular Biochemistry of Fungi, Institute for Chemical Engineering, Vienna University of Technology, Getreidemarkt 9, A 1060 Wien, Austria
    Fungal Genet Biol 42:749-60. 2005
    ..atroviride...
  39. ncbi Heterotrimeric G protein alpha and beta subunits antagonistically modulate stomatal density in Arabidopsis thaliana
    Lingang Zhang
    Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, China
    Dev Biol 324:68-75. 2008
    ..Taken together, these results suggest that the stomatal density in Arabidopsis is modulated by GPA1 and AGB1 in an antagonistic manner...
  40. pmc Cryptococcus neoformans mating and virulence are regulated by the G-protein alpha subunit GPA1 and cAMP
    J A Alspaugh
    Department of Genetics, Duke University Medical Center, Durham, North Carolina 27710, USA
    Genes Dev 11:3206-17. 1997
    ..These observations support a model in which GPA1 has a role in sensing diverse environmental signals required for mating and virulence by regulating cAMP metabolism in C. neoformans...
  41. pmc The information highways of a biotechnological workhorse--signal transduction in Hypocrea jecorina
    Monika Schmoll
    Research Area of Gene Technology and Applied Biochemistry, Institute for Chemical Engineering, Vienna University of Technology, Wien, Austria
    BMC Genomics 9:430. 2008
    ..A better understanding of these signals as well as their transmission machinery can provide sources for improvement of biotechnological processes...
  42. pmc Guanine nucleotide dissociation inhibitor activity of the triple GoLoco motif protein G18: alanine-to-aspartate mutation restores function to an inactive second GoLoco motif
    Randall J Kimple
    Department of Pharmacology, Lineberger Comprehensive Cancer Center, and UNC Neuroscience Center, The University of North Carolina, Chapel Hill, NC 27599, USA
    Biochem J 378:801-8. 2004
    ....
  43. pmc Speed, spatial, and temporal tuning of rod and cone vision in mouse
    Yumiko Umino
    Center for Vision Research, Department of Ophthalmology, State University of New York, Upstate Medical University, Syracuse, New York 13210, USA
    J Neurosci 28:189-98. 2008
    ..This parametric study characterizes the functional properties of the mouse visual system, revealing the rod and cone contributions to contrast sensitivity and to the temporal processing of visual stimuli...
  44. pmc Structural and dynamical changes in an alpha-subunit of a heterotrimeric G protein along the activation pathway
    Ned Van Eps
    Jules Stein Eye Institute, Departments of Ophthalmology and Chemistry and Biochemistry, University of California Los Angeles, Los Angeles, CA 90095, USA
    Proc Natl Acad Sci U S A 103:16194-9. 2006
    ..A previously unreported activation-dependent change in alpha4, distant from the interaction surface, supports a role for this helix in effector binding...
  45. ncbi Drosophila RhoGEF2 associates with microtubule plus ends in an EB1-dependent manner
    Stephen L Rogers
    Howard Hughes Medical Institute and Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA 94107, USA
    Curr Biol 14:1827-33. 2004
    ....
  46. pmc Formation of atroviridin by Hypocrea atroviridis is conidiation associated and positively regulated by blue light and the G protein GNA3
    Monika Komon-Zelazowska
    Technische Universitat Wien, Institut fur Verfahrenstechnik, Umwelttechnik, und Technische Biowissenschaften, FB Gentechnik und Angewandte Biochemie, Getreidemarkt 9 166, 1060 Wien, Austria
    Eukaryot Cell 6:2332-42. 2007
    ..Our data show that formation of atroviridin by H. atroviridis occurs in a sporulation-associated manner but is uncoupled from it at the stage of GNA3...
  47. ncbi G proteins Go green: a plant G protein signaling FAQ sheet
    Sarah M Assmann
    Biology Department, Pennsylvania State University, 208 Mueller Laboratory, University Park, PA 16802, USA
    Science 310:71-3. 2005
    ..Pathways in Science's Signal Transduction Knowledge Environment Connections Maps database provide details about the emerging roles of G proteins in several cellular processes of plants...
  48. ncbi Mutations in the Dictyostelium heterotrimeric G protein alpha subunit G alpha5 alter the kinetics of tip morphogenesis
    J A Hadwiger
    Department of Biology, Center for Molecular Genetics, University of California, San Diego, La Jolla 92093 0634, USA
    Development 122:1215-24. 1996
    ..Overexpression of these mutant G alpha5 genes by increased gene dosage resulted in cell death, suggesting that high levels of the altered subunits have detrimental effects during vegetative growth...
  49. pmc The G-alpha protein GNA3 of Hypocrea jecorina (Anamorph Trichoderma reesei) regulates cellulase gene expression in the presence of light
    Monika Schmoll
    Research Area Gene Technology and Applied Biochemistry, Institute for Chemical Engineering, Vienna University of Technology, Getreidemarkt 9 1665, Vienna 1060, Austria
    Eukaryot Cell 8:410-20. 2009
    ..Our data for the first time imply an involvement of a G-alpha subunit in a light-dependent signaling event in fungi...
  50. pmc The plastid protein THYLAKOID FORMATION1 and the plasma membrane G-protein GPA1 interact in a novel sugar-signaling mechanism in Arabidopsis
    Jirong Huang
    Department of Biology, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    Plant Cell 18:1226-38. 2006
    ..The interaction between THF1 and GPA1 has been confirmed by in vitro and in vivo coimmunoprecipitation, FRET analysis, and genetic epistasis and provides evidence of a sugar-signaling mechanism between plastids and the plasma membrane...
  51. ncbi A G protein-coupled receptor is a plasma membrane receptor for the plant hormone abscisic acid
    Xigang Liu
    National Institute of Biological Sciences, 7 Science Park Road, Zhongguancun Life Science Park, Beijing 102206, China
    Science 315:1712-6. 2007
    ..The binding of ABA to the receptor leads to the dissociation of the receptor-GPA1 complex in yeast. Our results demonstrate that this G protein-coupled receptor is a plasma membrane ABA receptor...
  52. ncbi Purification and in vitro functional analysis of the Arabidopsis thaliana regulator of G-protein signaling-1
    Francis S Willard
    Department of Pharmacology, The University of North Carolina at Chapel Hill, 27599 7365, USA
    Methods Enzymol 389:320-38. 2004
    ..This article describes methods for the purification and in vitro functional analysis of the RGS box of AtRGS1...
  53. pmc Steroids initiate a signaling cascade that triggers rapid sporulation in Dictyostelium
    Christophe Anjard
    Center for Molecular Genetics, Division of Biological Sciences, University of California San Diego, La Jolla, CA 92093, USA
    Development 136:803-12. 2009
    ..SDF-3 is at the head of the cascade that amplifies the signal for encapsulation to ensure the rapid, synchronous formation of spores...
  54. pmc Light regulation of retinal dopamine that is independent of melanopsin phototransduction
    M A Cameron
    Faculty of Life Sciences, University of Manchester, Manchester, UK
    Eur J Neurosci 29:761-7. 2009
    ..Our data suggest that light regulation of DA, a pivotal retinal neuromodulator, originates primarily with rods and cones, and that melanopsin is neither necessary nor sufficient for this photoresponse...
  55. pmc Role of a heterotrimeric G protein in regulation of Arabidopsis seed germination
    Hemayet Ullah
    Department of Agronomy, Nanjing Agricultural University, Nanjing 210095, People s Republic of China
    Plant Physiol 129:897-907. 2002
    ..A lack of BR responsiveness is also apparent in gpa1 roots and hypocotyls suggesting that BR signal transduction is likely coupled by a heterotrimeric G protein at various points in plant development...
  56. ncbi LET-99, GOA-1/GPA-16, and GPR-1/2 are required for aster-positioned cytokinesis
    Henrik Bringmann
    Max Planck Institute for Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307 Dresden, Germany
    Curr Biol 17:185-91. 2007
    ..We conclude that LET-99 and the G proteins define a molecular pathway required for aster-positioned cytokinesis...
  57. pmc Kelch-repeat proteins interacting with the Galpha protein Gpa2 bypass adenylate cyclase for direct regulation of protein kinase A in yeast
    Tom Peeters
    Laboratory of Molecular Cell Biology, Katholieke Universiteit Leuven, and Department of Molecular Microbiology, Flanders Interuniversity Institute of Biotechnology, Kasteelpark Arenberg 31, B 3001 Leuven Heverlee, Belgium
    Proc Natl Acad Sci U S A 103:13034-9. 2006
    ..Importantly, we show that Krh1/2 also enhance the association between mouse R and C subunits, suggesting that Krh control of PKA has been evolutionarily conserved...
  58. ncbi Analysis of altered G-protein subunit accumulation in Cryphonectria parasitica reveals a third Galpha homologue
    Todd B Parsley
    Center for Biosystems Research formerly The Center for Agricultural Biotechnology, University of Maryland Biotechnology Institute, 5115 Plant Sciences Building, College Park, MD 20742, USA
    Curr Genet 43:24-33. 2003
    ..We describe the sequence of a new Galpha subunit, CPG-3, that is most similar to three other filamentous fungal Galpha proteins that form a phylogenetically distinct grouping...
  59. pmc A reevaluation of the role of the heterotrimeric G protein in coupling light responses in Arabidopsis
    Alan M Jones
    The Salk Institute for Biological Studies, La Jolla, California 92037, USA
    Plant Physiol 131:1623-7. 2003
    ..These results preclude a direct role for the heterotrimeric G complex in R and FR transduction in Arabidopsis leading to growth control in the hypocotyl...
  60. ncbi RGS-Rz and RGS9-2 proteins control mu-opioid receptor desensitisation in CNS: the role of activated Galphaz subunits
    Pilar Sánchez-Blázquez
    Neuropharmacology, Cajal Institute, CSIC, Dr Arce 37, E 28002 Madrid, Spain
    Neuropharmacology 48:134-50. 2005
    ..g., through the activity of RGS9-2 proteins...
  61. pmc Yeast pseudohyphal growth is regulated by GPA2, a G protein alpha homolog
    M C Lorenz
    Department of Genetics, Duke University Medical Center, Durham, NC 27710, USA
    EMBO J 16:7008-18. 1997
    ..Our findings suggest that GPA2 is an element of the nitrogen sensing machinery that regulates pseudohyphal differentiation by modulating cAMP levels...
  62. ncbi The Galpha protein Gpa2 controls yeast differentiation by interacting with kelch repeat proteins that mimic Gbeta subunits
    Toshiaki Harashima
    Department of Molecular Genetics and Microbiology, Durham, North Carolina 27710, USA
    Mol Cell 10:163-73. 2002
    ..Our studies demonstrate that Gpa2 signals in conjunction with Gbeta structural mimics and that homologous G protein subunits or effectors may be conserved in multicellular eukaryotes...
  63. ncbi G-protein coupled receptor from yeast Saccharomyces cerevisiae
    C W Yun
    Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Japan
    Biochem Biophys Res Commun 240:287-92. 1997
    ..It is likely that a Gpr1p monitors the extracellular signal such as nutrition and transduce it via Gpa2p a possible positive regulator of cAMP level...
  64. pmc The heterotrimeric G-protein GanB(alpha)-SfaD(beta)-GpgA(gamma) is a carbon source sensor involved in early cAMP-dependent germination in Aspergillus nidulans
    Anne Lafon
    Unité Postulante Biologie et Pathogénicité Fongiques, INRA USC2019, Institut Pasteur, Paris, France
    Genetics 171:71-80. 2005
    ..Furthermore, GanB may function in sensing various carbon sources and subsequent activation of downstream signaling for germination...
  65. pmc The plant innate immunity response in stomatal guard cells invokes G-protein-dependent ion channel regulation
    Wei Zhang
    Biology Department, Penn State University, University Park, PA 16802 5301, USA
    Plant J 56:984-96. 2008
    ..These new findings provide insights into the largely elusive signaling process underlying PTI-associated guard cell responses...
  66. pmc Abscisic acid regulation of guard-cell K+ and anion channels in Gbeta- and RGS-deficient Arabidopsis lines
    Liu Min Fan
    Biology Department, Penn State University, University Park, PA 16802 5301
    Proc Natl Acad Sci U S A 105:8476-81. 2008
    ..Our studies highlight unique aspects of ion channel regulation by heterotrimeric G proteins and relate these aspects to stomatal aperture control, a key determinant of plant biomass acquisition and drought tolerance...
  67. ncbi Influence of Gz and Gi2 transducer proteins in the affinity of opioid agonists to mu receptors
    J Garzon
    Neurofarmacologia, Instituto Cajal, Consejo Superior de Investigaciones Cientificas, C Dr Arce 37, 28002 Madrid, Spain
    Eur J Neurosci 10:2557-64. 1998
    ..Therefore, the affinity exhibited by opioid agonists of mu-ORs, but not antagonists, depends on the nature of the G-protein coupled to these receptors...
  68. ncbi Sst2 is a GTPase-activating protein for Gpa1: purification and characterization of a cognate RGS-Galpha protein pair in yeast
    D M Apanovitch
    Department of Pharmacology and Molecular Cardiobiology Program, Boyer Center for Molecular Medicine, Yale University School of Medicine, New Haven, Connecticut 06536, USA
    Biochemistry 37:4815-22. 1998
    ..These experiments represent the first biochemical characterization of Gpa1 and Sst2, and provide a molecular basis for their well-established biological roles in signaling and desensitization...
  69. pmc G proteins in Ustilago maydis: transmission of multiple signals?
    E Regenfelder
    Institut für Genetik und Mikrobiologie der Universität München, Germany
    EMBO J 16:1934-42. 1997
    ..Surprisingly, Gpa3 is also required for pathogenic development, although pheromone signalling is not essential for this process...
  70. ncbi Signaling via the G protein alpha subunit FGA2 is necessary for pathogenesis in Fusarium oxysporum
    Sona Jain
    Faculty of Agriculture, Ehime University, Matsuyama, 790 8566, Japan
    FEMS Microbiol Lett 243:165-72. 2005
    ..The fga1/fga2 double disruptants showed phenotypic alterations similar to the fga1 or fga2 single disruptants, but increase of heat resistance was much more pronounced than in each single disruptant...
  71. pmc Overexpression of the heterotrimeric G-protein alpha-subunit enhances phytochrome-mediated inhibition of hypocotyl elongation in Arabidopsis
    H Okamoto
    Department of Molecular, Cellular, and Developmental Biology, Yale University, 165 Prospect Street, New Haven, CT 06520-8104, USA
    Plant Cell 13:1639-52. 2001
    ..Thus, our results support the involvement of a heterotrimeric G-protein in the light regulation of Arabidopsis seedling development...
  72. ncbi Arabidopsis phospholipase Dalpha1 interacts with the heterotrimeric G-protein alpha-subunit through a motif analogous to the DRY motif in G-protein-coupled receptors
    Jian Zhao
    Department of Biochemistry, Kansas State University, Manhattan, KS 66506, USA
    J Biol Chem 279:1794-800. 2004
    ....
  73. ncbi The Leu-132 of the Ste4(Gbeta) subunit is essential for proper coupling of the G protein with the Ste2 alpha factor receptor during the mating pheromone response in yeast
    L Ongay-Larios
    Departamento de Genetica Molecular, Instituto de Fisiologia Celular, Universidad Nacional Autonoma de Mexico, P O Box 70 242, 04510, Mexico, Mexico
    FEBS Lett 467:22-6. 2000
    ....
  74. pmc Genetic identification of residues involved in association of alpha and beta G-protein subunits
    M Whiteway
    National Research Council, Biotechnology Research Institute, Montreal, Quebec, Canada
    Mol Cell Biol 14:3223-9. 1994
    ..Because the ability of the modified G alpha subunit to suppress the Ste4 mutations is allele specific, it is likely that the residues defined by this analysis play a direct role in G-protein subunit association...
  75. pmc Substitutions in the pheromone-responsive Gbeta protein of Saccharomyces cerevisiae confer a defect in recovery from pheromone treatment
    E Li
    Laboratory for Molecular Biology, University of Illinois at Chicago, 60607, USA
    Genetics 148:947-61. 1998
    ..On the basis of these observations, we propose that Gpa1p-mediated adaptation involves the binding of an unknown negative regulator to Gbetagamma...
  76. ncbi Purification of G protein subunits from Sf9 insect cells using hexahistidine-tagged alpha and beta gamma subunits
    Tohru Kozasa
    Department of Pharmacology, University of Illinois at Chicago, USA
    Methods Mol Biol 237:21-38. 2004
    ..Using Sf9-Baculovirus expression system, a general and simplified method to purify various G protein subunits is described in this chapter. This method is useful for purification of most of G protein subunits...
  77. ncbi G protein signaling in yeast: new components, new connections, new compartments
    Janna E Slessareva
    Department of Biochemistry and Biophysics, University of North Carolina, Chapel Hill, NC 27599 7260, USA
    Science 314:1412-3. 2006
    ..New tools available in yeast are beginning to uncover new pathway components and interactions and have revealed signaling in unexpected locations within the cell...
  78. ncbi Galpha protein dependent and independent effects of human RGS1 expression in yeast
    Xiao Yu Li
    Department of Anatomy and Cell Biology, McGill University, Montreal, Quebec, Canada
    Cell Signal 16:43-9. 2004
    ..Taken together, our results indicate that RGS1 can function in both G-protein-dependent and -independent manners in yeast...
  79. ncbi Activation of serum response element by D2 dopamine receptor is governed by Gbetagamma-mediated MAPK and Rho pathways and regulated by RGS proteins
    Guyu Ho
    Wyeth Neuroscience Research, Wyeth Research, Princeton, N J, USA
    Pharmacology 79:114-21. 2007
    ..These results delineate the signaling pathways which couple D2 receptor to the transcriptional activation of SRE and demonstrate a modulatory role for RGS proteins in these processes...
  80. ncbi Selective interactions between G protein subunits and RGS4 with the C-terminal domains of the mu- and delta-opioid receptors regulate opioid receptor signaling
    Zafiroula Georgoussi
    Laboratory of Cellular Signaling and Molecular Pharmacology, Institute of Biology, National Center for Scientific Research Demokritos Ag Paraskevi 15310, Athens, Greece
    Cell Signal 18:771-82. 2006
    ..Collectively, our data demonstrate that the C-termini of the mu- and delta-ORs provide direct physical scaffolding in which G protein subunits and RGS4 protein can be bound...
  81. pmc The GAPs, GEFs, and GDIs of heterotrimeric G-protein alpha subunits
    David P Siderovski
    Department of Pharmacology, UNC Lineberger Comprehensive Cancer Center, and UNC Neuroscience Center, The University of North Carolina at Chapel Hill, CB 7365, 1106 M E Jones Building, Chapel Hill, NC 27599 7365 USA
    Int J Biol Sci 1:51-66. 2005
    ..Discovery of these novel GAP, GDI, and GEF activities have helped to illuminate a new role for Galpha subunit GDP/GTP cycling required for microtubule force generation and mitotic spindle function in chromosomal segregation...
  82. ncbi Translating G protein subunit diversity into functional specificity
    Janet D Robishaw
    100 N Academy Ave, Weis Center for Research, Geisinger Clinic, Danville, PA 17822 2614, USA
    Curr Opin Cell Biol 16:206-9. 2004
    ..Although much remains to be learned, the assembly of specific alphabetagamma subunit combinations seems to involve both structural and spatial factors...
  83. ncbi The Gbeta(KlSte4p) subunit of the heterotrimeric G protein has a positive and essential role in the induction of mating in the yeast Kluyveromyces lactis
    Laura Kawasaki
    Departamento de Genetica Molecular, Instituto de Fisiologia Celular, Universidad Nacional Autónoma de México Apartado Postal 70 242 04510 México, D F, Mexico
    Yeast 22:947-56. 2005
    ..cerevisiae but fails to restore the mating deficiency of Scste4Delta mutant. The data presented indicate that the mating pathway of K. lactis is positively and cooperatively regulated by both the Galpha and the Gbeta subunits...
  84. ncbi Pertussis-toxin-sensitive Galpha subunits selectively bind to C-terminal domain of neuronal GIRK channels: evidence for a heterotrimeric G-protein-channel complex
    Sinead M Clancy
    Peptide Biology Laboratory, The Salk Institute for Biological Studies, 10010 N Torrey Pines Road, La Jolla, CA 92037, USA
    Mol Cell Neurosci 28:375-89. 2005
    ..Together, these results suggest that the interaction of PTX-sensitive Galphai/o subunit with the GIRK2 C-terminal domain regulates G-protein receptor coupling, and may be important for establishing specific Galphai/o signaling pathways...
  85. ncbi Ultra-low-dose naloxone suppresses opioid tolerance, dependence and associated changes in mu opioid receptor-G protein coupling and Gbetagamma signaling
    H Y Wang
    Department of Physiology and Pharmacology, City University of New York Medical School, 138th Street and Convent Avenue, New York, NY 10031, USA
    Neuroscience 135:247-61. 2005
    ..These findings provide a molecular mechanism underpinning opioid tolerance and dependence and their attenuation by ultra-low-dose opioid antagonists...
  86. pmc Biochemical characterization of RGS14: RGS14 activity towards G-protein alpha subunits is independent of its binding to Rap2A
    Vivek Mittal
    Department of Cell Biology and Physiology, Washington University School of Medicine, 660 South Euclid Ave, Campus Box 8228, St Louis, MO 63110, USA
    Biochem J 394:309-15. 2006
    ..This is consistent with models of GoLoco domain action on free G(alpha) and suggests that RGS14 alone cannot induce subunit dissociation to promote receptor-independent activation of G(betagamma)-mediated signalling pathways...
  87. pmc Heterotrimeric G-proteins: a short history
    Graeme Milligan
    Molecular Pharmacology Group, Division of Biochemistry and Molecular Biology, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow G12 8QQ
    Br J Pharmacol 147:S46-55. 2006
    ..Finally, further modifications of such chimeras have generated a range of G-protein alpha-subunits with greater promiscuity to interact across GPCR classes and initiated the use of such modified G-proteins in drug discovery programmes...
  88. pmc Galpha subunit Gpa2 recruits kelch repeat subunits that inhibit receptor-G protein coupling during cAMP-induced dimorphic transitions in Saccharomyces cerevisiae
    Toshiaki Harashima
    Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, NC 27710, USA
    Mol Biol Cell 16:4557-71. 2005
    ..Our studies invoke novel mechanisms involving GPCR-G protein modules that may be conserved in multicellular eukaryotes...
  89. ncbi Loss of association between activated Galpha q and Gbetagamma disrupts receptor-dependent and receptor-independent signaling
    Daniel S Evanko
    Department of Microbiology and Immunology, Kimmel Cancer Center, Thomas Jefferson University, 233 S 10th St, 839 BLSB, Philadelphia, PA 19107, USA
    Cell Signal 17:1218-28. 2005
    ..Taken together, results presented here suggest that alpha-betagamma binding is necessary at a point downstream from receptor activation of the heterotrimeric G protein for signal transduction by alpha q...
  90. ncbi Mathematical modeling of RGS and G-protein regulation in yeast
    Necmettin Yildirim
    Department of Mathematics, University of North Carolina at Chapel Hill, 27599 3250, USA
    Methods Enzymol 389:383-98. 2004
    ..It also illustrates that a complete understanding of signaling pathways requires an investigation of their time-dependent behavior...
  91. pmc Mechanistic pathways and biological roles for receptor-independent activators of G-protein signaling
    Joe B Blumer
    Department of Pharmacology and Experimental Therapeutics, Louisiana State University Health Sciences Center, 1901 Perdido Street, New Orleans, LA 70112, United States
    Pharmacol Ther 113:488-506. 2007
    ....
  92. ncbi Interaction of Gbetagamma with RACK1 and other WD40 repeat proteins
    Songhai Chen
    Department of Pharmacology, Vanderbilt University Medical Center, Room 442, Robinson Research Building, Nashville, TN 37232, USA
    J Mol Cell Cardiol 37:399-406. 2004
    ..Here we will describe the molecular mechanism underlying this interaction and the implications of the interaction on the signal transduction of G-protein and RACK1...
  93. pmc Schizosaccharomyces pombe zfs1+ encoding a zinc-finger protein functions in the mating pheromone recognition pathway
    J Kanoh
    Department of Biophysics and Biochemistry, School of Science, University of Tokyo, Japan
    Mol Biol Cell 6:1185-95. 1995
    ..These observations indicate that the function of zfs1 is involved in the mating pheromone signaling pathway, and are consistent with its function being required to fully activate a factor in this pathway, either directly or indirectly...
  94. pmc Heterotrimeric G protein signaling in the Arabidopsis unfolded protein response
    Shiyu Wang
    Biology Department and Huck Institutes of the Life Sciences, Pennsylvania State University, University Park, PA 16802, USA
    Proc Natl Acad Sci U S A 104:3817-22. 2007
    ....
  95. ncbi Truncated forms of a novel yeast protein suppress the lethality of a G protein alpha subunit deficiency by interacting with the beta subunit
    B H Spain
    Department of Biological Chemistry, UCLA School of Medicine 90024, USA
    J Biol Chem 270:25435-44. 1995
    ..The properties of Syg1p are consistent with those of a transmembrane signaling component that can respond to, or transduce signals through, G beta or G beta gamma...
  96. pmc Galpha Gbetagamma dissociation may be due to retraction of a buried lysine and disruption of an aromatic cluster by a GTP-sensing Arg Trp pair
    Andrew F Neuwald
    J Craig Venter Institute, Rockville, MD 20850, USA
    Protein Sci 16:2570-7. 2007
    ..Other structural features associated with Galpha functional divergence further support the proposed mechanism...
  97. pmc Distinct roles for two Galpha-Gbeta interfaces in cell polarity control by a yeast heterotrimeric G protein
    Shelly C Strickfaden
    Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Mol Biol Cell 19:181-97. 2008
    ..These findings raise the possibility that the Galphabetagamma heterotrimer can function in a partially dissociated state, tethered by the N-terminal interface...
  98. pmc Signal transduction by a nondissociable heterotrimeric yeast G protein
    S Klein
    Department of Biological Chemistry, Alexander Silverman Institute of Life Sciences, The Hebrew University of Jerusalem, Givat Ram, Jerusalem 91904, Israel
    Proc Natl Acad Sci U S A 97:3219-23. 2000
    ..Hence, dissociation of this G protein is not required for its activation. Rather, a conformational change in the heterotrimeric complex is likely to be involved in signal transduction...
  99. ncbi Characterization of umami receptor and coupling G protein in mouse taste cells
    Masataka Narukawa
    Graduate School of Agriculture, Kyoto University, Uji, Japan
    Neuroreport 19:1169-73. 2008
    ..Thus, we demonstrated that umami stimuli were received by G protein-coupled receptors that function together with some of the Galphai family members...
  100. pmc Mapping of a yeast G protein betagamma signaling interaction
    S J Dowell
    Glaxo Wellcome Research and Development, Stevenage, SG1 2NY, United Kingdom
    Genetics 150:1407-17. 1998
    ..Identification of the Gbetagamma coiled-coil in Ste5p binding may set a precedent for Gbetagamma-effector interactions in more complex organisms...
  101. ncbi Sequence elements that contribute to the degradation of yeast G alpha
    C Schauber
    Department of Biochemistry, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, Piscataway 08854, USA
    Genes Cells 3:307-19. 1998
    ..It is likely that the signal transduced by Gbetagamma can be regulated by adjusting the levels of G alpha through proteolysis...

Research Grants55

  1. G(alpha)Z signaling in insulin secretion and glucose tolerance
    Michelle E Kimple; Fiscal Year: 2010
    ..How much insulin is secreted into the blood is one determinant of blood glucose levels;therefore, this project has direct relevance to diabetes. ..
  2. Mechanisms of Mitotic Spindle Orientation in Mammalian Cells
    Quansheng Du; Fiscal Year: 2011
    ..Results from the proposed studies will advance our understanding of the molecular mechanisms by which spindle orientation and asymmetric cell division are achieved in mammalian cells. ..
  3. Biochemical and Cell-Based HTS Assays for RGS17 Inhibitors
    David L Roman; Fiscal Year: 2013
    ....
  4. SIGNAL TRANSDUCTION IN T CELLS
    CONSTANTINE TSOUKAS; Fiscal Year: 1991
    ..We'll measure the effects of these agents on IP3 and Ca++ accumulation using a permeabilized cell system and electroporation...
  5. MOLECULAR ANALYSIS OF G PROTEIN ALPHA SUBUNIT FUNCTIONS
    CATHERINE BERLOT; Fiscal Year: 1999
    ....
  6. Identification of factors controlling the genesis of cone photoreceptors
    Mark Emerson; Fiscal Year: 2009
    ..A basic understanding of the normal mechanisms that generate photoreceptors will provide the basis for replacing these cells with new functional photoreceptors. ..
  7. DICTYOSTELIUM GA2:CELLULAR LOCALIZATION & PALMITOYLATION
    ROBERT GUNDERSEN; Fiscal Year: 2000
    ..The genes for these enzymes will be identified using restriction enzyme-mediated integration (REMI) mutagenesis coupled to screens designed to isolate mutations in G-alpha-2 function. ..
  8. Biochemical and Genetic Analysis of the Visual Cycle
    GABRIEL TRAVIS; Fiscal Year: 2009
    ....
  9. G PROTEIN-MEDIATED NUTRITIONAL SIGNALING IN YEAST
    JEANNE HIRSCH; Fiscal Year: 2003
    ..Such information is essential for understanding the process of carcinogenesis, in which cells escape this regulation and exhibit uncontrolled growth. ..
  10. INHIBITION OF G PROTEIN BETA-SUBUNIT SIGNALING IN YEAST
    JEANNE HIRSCH; Fiscal Year: 2004
    ..abstract_text> ..
  11. A KNOCK-IN MOUSE FOR MARKING AND ISOLATION OF ALPHA-GUST
    Sami Damak; Fiscal Year: 2003
    ....
  12. IDENTIFICATION OF A G PROTEIN IN PI PATHWAY IN T CELLS
    MARTHA GRABER; Fiscal Year: 1992
    ..The long-term goal is to apply the skills and knowledge gained during this award to the study of T cell-mediated immune mechanisms in renal disease and/or transplantation...
  13. TRIMERIC G PROTEINS AND CONTROL OF HOMEOSTASIS
    Mariel Birnbaumer; Fiscal Year: 2001
    ..The elucidation of the mechanism(s) altered by lack of Go may aid in the understanding of human motor disorders and may shed new light on development and/or treatment of type II diabetes. ..
  14. G PROTEINS IN THE CNS
    Michael Forte; Fiscal Year: 1991
    ..Using the system we will establish, G proteins will be, for the first time, subjected to study by a number of combined approaches in vivo to determine the role they play in nervous system function and development...
  15. G-protein signal coordination by RGS12
    DAVID SIDEROVSKI; Fiscal Year: 2009
    ..We wish to study one RGS protein, RGS12, that interferes with the actions of the neurotransmitter GABA in neurons that control pain processing. This work should lead to the discovery of new pain-controlling drugs. ..
  16. Protanoids, Colitis, and Colon Cancer in Gia2-KO mice
    Robert Edwards; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  17. Structural Analysis of the Rhodopsin-Transducin Complex
    KEVIN RIDGE; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  18. Chemokine Signaling and Colitis in Gia2 Deficient Mice
    Robert Edwards; Fiscal Year: 2006
    ..This application has the support of outstanding mentors at two institutions, and funding of this application will foster Dr Edwards' continued development as a physician-scientist. ..
  19. Real-Time Fluorescence Assay:RGS Domain GAP Activit(RMI)
    DAVID SIDEROVSKI; Fiscal Year: 2008
    ..unreadable]PERFORMANCE SITE ========================================Section End=========================================== ..
  20. Advanced Sequence-Based Prediction of Protein Function
    ANDREW NEUWALD; Fiscal Year: 2005
    ..abstract_text> ..
  21. Predicting common protein mechanisms by the light of evolution
    ANDREW NEUWALD; Fiscal Year: 2009
    ..And (v) propose molecular mechanisms based on these analyses. ..
  22. Mechanistic studies of a novel G-alpha nucleotide cycle
    DAVID SIDEROVSKI; Fiscal Year: 2009
    ....
  23. STRUCTURAL STUDIES OF G-PROTEIN COUPLED RECEPTORS
    KEVIN RIDGE; Fiscal Year: 2004
    ....
  24. G PROTEIN BETA GAMMA SIGNALING SYSTEMS IN VIVO
    JANET ROBISHAW; Fiscal Year: 2007
    ..Since many diseases (e.g. cancer, heart disease) are associated with re-induction of embryonic genes, this information will eventually lead to the better diagnosis and more selective therapeutic treatments. ..
  25. CRCNS:Comput./Exp.Study:Hypothal.-Pituitary Interaction
    Richard Bertram; Fiscal Year: 2008
    ..It will then lead to studies of effects of drugs of abuse on brain-pituitary function. [unreadable] [unreadable]..
  26. Membrane Targeting of G Proteins
    Philip Wedegaertner; Fiscal Year: 2007
    ..abstract_text> ..
  27. ASM Conference on Candida and Candidiasis
    Aaron Mitchell; Fiscal Year: 2008
    ..The conference will help chart the course of future research and facilitate the urgent necessity to understand, treat, and prevent disease. [unreadable] [unreadable] [unreadable]..
  28. The Third RGS Protein Colloquium
    MICHAEL KOELLE; Fiscal Year: 2008
    ..Meeting materials will be made available to the public via the conference web site to increase the impact of the Colloquium. [unreadable] [unreadable] [unreadable]..
  29. ALPHA1-ADRENERGIC SIGNALING PATHWAYS IN HEART
    JANET ROBISHAW; Fiscal Year: 2002
    ..These studies are critical for the design of new therapeutic strategies aimed at enhancing or counteracting alpha/1-AR subtype specific effects in the heart. ..
  30. Beta/Gamma Subunit Heterogeniety in G Proteins
    Janet D Robishaw; Fiscal Year: 2010
    ....
  31. HIGH-THROUGHPUT SCEENS FOR ASSESSING TASTE SENSITIVITY
    JOHN GLENDINNING; Fiscal Year: 2001
    ..A normative data-base for both screens will be established and made available to the scientific community. The assay procedures developed will be applicable by a single laboratory technician with limited experience. ..
  32. Chemosensing in the Gastrointestinal Tract
    Catia Sternini; Fiscal Year: 2010
    ....
  33. HORMONE SECRETION AND PROCESSING IN PITUITARY CELLS
    Marilyn Gist Farquhar; Fiscal Year: 2010
    ..These studies can be expected to considerably expand knowledge of the role of G proteins in regulated secretion of hormones and in endocytosis and signaling of hormones by GPCR. ..
  34. Sphingolipid Regulation of Caspase 9 Alternative Splicing
    Charles Chalfant; Fiscal Year: 2009
    ..This cannot be understated because the definition of these signal transduction pathways creates, not one, but many new targets, for anti-cancer therapies. ..
  35. Control of Meiotic Homolog Segregation in Arabidopsis
    Hong Ma; Fiscal Year: 2004
    ..Furthermore, molecular and cell biological experiments will be done to characterize the expression pattern and protein localization of SDS, and to isolate a putative SDS-like gene. ..
  36. mu Opioid Receptor Function in the Enteric Nervous System
    Catia Sternini; Fiscal Year: 2007
    ..abstract_text> ..
  37. High Gulcose Promotes Myocyte Apoptosis by PKC Pathways
    Ashwani Malhotra; Fiscal Year: 2008
    ..The proposed investigations are novel and fundamental to the development of gene based therapy to inhibit myocyte apoptosis in the diabetic heart. ..
  38. New strategies for identification of C. albicans fluconazole resistance gene
    Aaron Mitchell; Fiscal Year: 2004
    ..These approaches will define the regulators that govern known resistance mechanisms and may reveal the regulators that govern other clinically relevant resistance mechanisms. ..
  39. A Neuropeptide Gene in Psychiatric Illness
    Wade Berrettini; Fiscal Year: 2004
    ..We have assembled sets (n>600) of parent-child trios for both SZ and BPD to test the hypothesis that variation in this gene increases risks for these disorders. These studies may identify a susceptibility gene for SZ and/or BPD. ..
  40. QUANTITATIVE GENETICS OF OPIATE ADDICTION
    Wade Berrettini; Fiscal Year: 2004
    ..This may enhance our knowledge of genetic susceptibility to opioid dependence in human populations. ..
  41. RAD51 gene functions in mitosis and meiosis of zebrafish
    Hong Ma; Fiscal Year: 2004
    ..We will test this hypothesis using morpholino knockdowns of these genes. These experiments will pave the way for more extensive studies in the near future. ..
  42. Nano-HPLC-ESI Quadrupole Ion Trap Mass Spectrometer
    Alan Smrcka; Fiscal Year: 2005
    ..A cost recovery plan will be implemented that will cover costs in future years. ..
  43. Genetics of Anorexia Nervosa
    Wade Berrettini; Fiscal Year: 2005
    ..The diagnostic and genetic data and lymphoblastoid cell lines (derived from blood samples) will become part of a national archival resource for genetic studies of AN through the NIMH Genetics Initiative. ..
  44. The role of nyctalopin in the mammalian retina
    CATHERINE MORGANS; Fiscal Year: 2006
    ..unreadable] [unreadable] [unreadable]..
  45. FGF receptor signaling specificity in C.elegans
    MICHAEL KOELLE; Fiscal Year: 2007
    ..In this way, we will gain a clearer understanding of how specific responses are elicited by the activation of FGF receptors. ..
  46. A COLLABORATIVE GENOMIC STUDY OF BIPOLAR DISORDER
    Wade Berrettini; Fiscal Year: 2007
    ..Analysis of the existing sib pair families plus this large set of cases and controls should permit the confirmation of several vulnerability genes during this grant period. ..
  47. GASTRIC NEUROPEPTIDASES/PEPTIDE RECEPTOR INTERACTIONS
    Catia Sternini; Fiscal Year: 2002
    ..We also plan to characterize GRP receptor recycling and resensitization in cells that normally express GRP receptors: gastric G cells, Swiss 3T3 cells, and small cell lung carcinoma line NCI-H345 cells. ..
  48. South Eastern Region Lipid Conference 2006
    Charles Chalfant; Fiscal Year: 2007
    ..The funds requested in this proposal will make an invaluable contribution to the support of this conference. [unreadable] [unreadable] [unreadable]..
  49. ROLE OF GALANIN RECEPTORS IN GASTROINTESTINAL MOTILITY
    Catia Sternini; Fiscal Year: 2006
    ..abstract_text> ..
  50. STRUCTURAL BASIS FOR BINDING TO G PROTEIN BETA GAMMA
    Alan Smrcka; Fiscal Year: 2007
    ..4. Identification of sites of interaction G protein betagamma subunits with cellular targets using deuterium exchange mass spectrometry. ..
  51. MAP KINASE CASCADE SIGNAL TRANSMISSION AND SPECIFICITY
    Lee Bardwell; Fiscal Year: 2008
    ....
  52. Calcium channels at ribbon synapses in the retina
    CATHERINE MORGANS; Fiscal Year: 2008
    ..The results of these experiments will provide insight into the composition and functional organization of retinal ribbon synapses and into the perturbation of retinal function associated with CSNB2. ..
  53. Glomerular Capillaries-- Normal and Pathologic
    Marilyn Farquhar; Fiscal Year: 2008
    ..abstract_text> ..
  54. METHODS FOR FUNCTIONAL/COMPARATIVE GENOMICS
    Xun Gu; Fiscal Year: 2003
    ..abstract_text> ..