heterotrimeric gtp binding proteins

Summary

Summary: GTP-BINDING PROTEINS that contain three non-identical subunits. They are found associated with members of the seven transmembrane domain superfamily of G-PROTEIN-COUPLED RECEPTORS. Upon activation the GTP-BINDING PROTEIN ALPHA SUBUNIT of the complex dissociates leaving a dimer of a GTP-BINDING PROTEIN BETA SUBUNIT bound to a GTP-BINDING PROTEIN GAMMA SUBUNIT.

Top Publications

  1. ncbi GTPase-activating proteins for heterotrimeric G proteins: regulators of G protein signaling (RGS) and RGS-like proteins
    E M Ross
    Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, Texas 75390 9041, USA
    Annu Rev Biochem 69:795-827. 2000
  2. ncbi Heterotrimeric G proteins direct two modes of asymmetric cell division in the Drosophila nervous system
    M Schaefer
    Research Institute of Molecular Pathology I M P, Dr Bohr Gasse 7, 1030 Vienna, Austria
    Cell 107:183-94. 2001
  3. ncbi A seven-transmembrane RGS protein that modulates plant cell proliferation
    Jin Gui Chen
    Department of Biology, The University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 3280, USA
    Science 301:1728-31. 2003
  4. ncbi A protein complex containing Inscuteable and the Galpha-binding protein Pins orients asymmetric cell divisions in Drosophila
    M Schaefer
    Research Institute of Molecular Pathology IMP, Vienna, A 1030, Austria
    Curr Biol 10:353-62. 2000
  5. ncbi Mammalian sweet taste receptors
    G Nelson
    Howard Hughes Medical Institute, University of California at San Diego, La Jolla, CA 92093, USA
    Cell 106:381-90. 2001
  6. pmc Plants: the latest model system for G-protein research
    Alan M Jones
    Department of Biology, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    EMBO Rep 5:572-8. 2004
  7. pmc The beta-subunit of the Arabidopsis G protein negatively regulates auxin-induced cell division and affects multiple developmental processes
    Hemayet Ullah
    Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 3280, USA
    Plant Cell 15:393-409. 2003
  8. pmc Differential roles of Arabidopsis heterotrimeric G-protein subunits in modulating cell division in roots
    Jin Gui Chen
    Department of Botany, University of British Columbia, Vancouver, British Columbia V6T 1Z4, Canada
    Plant Physiol 141:887-97. 2006
  9. ncbi Crystal structure of rhodopsin: A G protein-coupled receptor
    K Palczewski
    Department of Ophthalmology, University of Washington, Seattle, WA 98195, USA
    Science 289:739-45. 2000
  10. ncbi A novel bifunctional phospholipase c that is regulated by Galpha 12 and stimulates the Ras/mitogen-activated protein kinase pathway
    I Lopez
    Department of Pathology and Feinberg Cardiovascular Research Institute, Northwestern University Medical School, Chicago, Illinois 60611, USA
    J Biol Chem 276:2758-65. 2001

Research Grants

  1. Coordinate Regulation Of Nuclear DGK-theta and PLD
    DANIEL RABEN; Fiscal Year: 2009
  2. BIOPHYSICS OF RECEPTOR/G-PROTEIN INTERACTIONS
    Oleg G Kisselev; Fiscal Year: 2011
  3. TRANSMEMBRANE SIGNALLING IN GENETIC OBESITY
    Kathryn LaNoue; Fiscal Year: 1993
  4. HETEROTRIMERIC G PROTEINS AND EXOCYTOSIS
    BHANU JENA; Fiscal Year: 1999
  5. PHOSPHOLIPASES AND INSULIN SECRETION
    Bryan Wolf; Fiscal Year: 1999
  6. REGULATION OF SIGNAL TRANSDUCTION IN DICTYOSTELIUM
    CHARLES SAXE; Fiscal Year: 2000
  7. PSGR as a Molecular Target in Human Prostate Cancer
    Mingyao Liu; Fiscal Year: 2005
  8. REGULATION OF ION CHANNELS BY SPECIFIC G PROTEINS
    MALGORZATA WILK BLASZCZAK; Fiscal Year: 1999
  9. Biophsyics of Receptor/G-protein Interactions
    OLEG KISSELEV; Fiscal Year: 2007
  10. Real-Time Fluorescence Assay:RGS Domain GAP Activit(RMI)
    DAVID SIDEROVSKI; Fiscal Year: 2008

Detail Information

Publications227 found, 100 shown here

  1. ncbi GTPase-activating proteins for heterotrimeric G proteins: regulators of G protein signaling (RGS) and RGS-like proteins
    E M Ross
    Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, Texas 75390 9041, USA
    Annu Rev Biochem 69:795-827. 2000
    ..GAPs are regulated by various controls of their cellular concentrations, by complex interactions with G¿ or with G¿5 through an endogenous G-like domain, and by interaction with multiple other proteins...
  2. ncbi Heterotrimeric G proteins direct two modes of asymmetric cell division in the Drosophila nervous system
    M Schaefer
    Research Institute of Molecular Pathology I M P, Dr Bohr Gasse 7, 1030 Vienna, Austria
    Cell 107:183-94. 2001
    ..Thus, asymmetric activation of heterotrimeric G proteins by a receptor-independent mechanism may orient asymmetric cell divisions in different cell types...
  3. ncbi A seven-transmembrane RGS protein that modulates plant cell proliferation
    Jin Gui Chen
    Department of Biology, The University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 3280, USA
    Science 301:1728-31. 2003
    ..These findings suggest that AtRGS1 is a critical modulator of plant cell proliferation...
  4. ncbi A protein complex containing Inscuteable and the Galpha-binding protein Pins orients asymmetric cell divisions in Drosophila
    M Schaefer
    Research Institute of Molecular Pathology IMP, Vienna, A 1030, Austria
    Curr Biol 10:353-62. 2000
    ..How apically localised Inscuteable directs asymmetric cell divisions is unclear...
  5. ncbi Mammalian sweet taste receptors
    G Nelson
    Howard Hughes Medical Institute, University of California at San Diego, La Jolla, CA 92093, USA
    Cell 106:381-90. 2001
    ..Finally, we present a detailed analysis of the patterns of expression of T1Rs and T2Rs, thus providing a view of the representation of sweet and bitter taste at the periphery...
  6. pmc Plants: the latest model system for G-protein research
    Alan M Jones
    Department of Biology, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    EMBO Rep 5:572-8. 2004
    ..Nonetheless, recent studies on Arabidopsis thaliana have shown the importance of plant G-protein signalling in such fundamental processes as cell proliferation, hormone perception and ion-channel regulation...
  7. pmc The beta-subunit of the Arabidopsis G protein negatively regulates auxin-induced cell division and affects multiple developmental processes
    Hemayet Ullah
    Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 3280, USA
    Plant Cell 15:393-409. 2003
    ..These results suggest that although auxin-regulated cell division is not coupled directly by a G protein, the Gbeta-subunit attenuates this auxin pathway upstream of the control of mRNA steady state levels...
  8. pmc Differential roles of Arabidopsis heterotrimeric G-protein subunits in modulating cell division in roots
    Jin Gui Chen
    Department of Botany, University of British Columbia, Vancouver, British Columbia V6T 1Z4, Canada
    Plant Physiol 141:887-97. 2006
    ..These results suggest that Arabidopsis heterotrimeric G-protein subunits have differential and opposing roles in the modulation of cell division in roots...
  9. ncbi Crystal structure of rhodopsin: A G protein-coupled receptor
    K Palczewski
    Department of Ophthalmology, University of Washington, Seattle, WA 98195, USA
    Science 289:739-45. 2000
    ..Identification of a set of residues that mediate interactions between the transmembrane helices and the cytoplasmic surface, where G-protein activation occurs, also suggests a possible structural change upon photoactivation...
  10. ncbi A novel bifunctional phospholipase c that is regulated by Galpha 12 and stimulates the Ras/mitogen-activated protein kinase pathway
    I Lopez
    Department of Pathology and Feinberg Cardiovascular Research Institute, Northwestern University Medical School, Chicago, Illinois 60611, USA
    J Biol Chem 276:2758-65. 2001
    ..Our findings demonstrate that PLCepsilon is a novel bifunctional enzyme that is regulated by the heterotrimeric G protein Galpha(12) and activates the small G protein Ras/mitogen-activated protein kinase signaling pathway...
  11. ncbi The plant heterotrimeric G-protein complex
    Brenda R S Temple
    R L Juliano Structural Bioinformatics Core Facility, Departments of Biology and Pharmacology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    Annu Rev Plant Biol 58:249-66. 2007
    ..This review addresses these unique features and speculates on why the repertoire of G-protein signaling elements is dramatically simpler than that in all other multicellular eukaryotes...
  12. ncbi Distinct roles for Galpha and Gbetagamma in regulating spindle position and orientation in Caenorhabditis elegans embryos
    M Gotta
    Wellcome CRC Institute, Tennis Court Road, Cambridge, CB2 1QR, UK
    Nat Cell Biol 3:297-300. 2001
    ..Gbetagamma is important in regulating migration of the centrosome around the nucleus and hence in orientating the mitotic spindle. Galpha is required for asymmetric spindle positioning in the one-celled embryo...
  13. ncbi An amino-acid taste receptor
    Greg Nelson
    Howard Hughes Medical Institute and Departments of Biology and Neurosciences, University of California at San Diego, La Jolla, California, 92093 0649, USA
    Nature 416:199-202. 2002
    ..We also show that sequence differences in T1R receptors within and between species (human and mouse) can significantly influence the selectivity and specificity of taste responses...
  14. pmc GTPase acceleration as the rate-limiting step in Arabidopsis G protein-coupled sugar signaling
    Christopher A Johnston
    Department of Pharmacology, Lineberger Comprehensive Cancer Center and Neuroscience Center, University of North Carolina, Chapel Hill, NC 27599 7365, USA
    Proc Natl Acad Sci U S A 104:17317-22. 2007
    ....
  15. ncbi Arabidopsis phospholipase Dalpha1 interacts with the heterotrimeric G-protein alpha-subunit through a motif analogous to the DRY motif in G-protein-coupled receptors
    Jian Zhao
    Department of Biochemistry, Kansas State University, Manhattan, KS 66506, USA
    J Biol Chem 279:1794-800. 2004
    ....
  16. pmc Expression of Galpha14 in sweet-transducing taste cells of the posterior tongue
    Marco Tizzano
    Rocky Mountain Taste and Smell Center, University of Colorado Denver School of Medicine, Aurora, CO 80045, USA
    BMC Neurosci 9:110. 2008
    ..We utilized RT-PCR, immunocytochemistry and single-cell gene expression profiling to examine the expression of the Galphaq family (q, 11, 14) in mouse taste buds...
  17. ncbi Downregulation of cone-specific gene expression and degeneration of cone photoreceptors in the Rpe65-/- mouse at early ages
    Sergey L Znoiko
    Departments of Ophthalmology, Medical University of South Carolina, Charleston, South Carolina, USA
    Invest Ophthalmol Vis Sci 46:1473-9. 2005
    ..Rod photoreceptors in the RPE65-knockout (Rpe65(-/-)) mouse are known to degenerate slowly with age. This study was designed to examine cone photoreceptors and the expression of cone-specific genes in the Rpe65(-/-) mouse...
  18. ncbi The distribution of active force generators controls mitotic spindle position
    Stephan W Grill
    Max Planck Institute of Molecular Cell Biology and Genetics, D 01307 Dresden, Germany
    Science 301:518-21. 2003
    ..Moreover, activation of heterotrimeric guanine nucleotide- binding protein (Gprotein) alpha subunits is required to generate these astral forces...
  19. ncbi Loss of G protein gamma 7 alters behavior and reduces striatal alpha(olf) level and cAMP production
    William F Schwindinger
    Weis Center for Research, Geisinger Clinic, Danville, Pennsylvania 17822, USA
    J Biol Chem 278:6575-9. 2003
    ..These data demonstrate that an individual gamma-subunit contributes to the specificity of a given signaling pathway and controls the formation or stability of a particular G protein heterotrimer...
  20. ncbi Chemotaxis: signalling the way forward
    Peter J M Van Haastert
    Department of Biochemistry, University of Groningen, Nijenborgh 4, 9747AG Groningen, The Netherlands
    Nat Rev Mol Cell Biol 5:626-34. 2004
    ..Recent studies are beginning to identify an intricate network of signalling molecules that mediate these processes, and how these molecules become localized in the cell is now becoming clear...
  21. ncbi G alpha14 is a candidate mediator of sweet/umami signal transduction in the posterior region of the mouse tongue
    Yoichiro Shindo
    Research Laboratories for Health and Gustatory Science, Asahi Breweries, Ltd, 1 1 21 Midori, Moriya, Ibaraki 302 0106, Japan
    Biochem Biophys Res Commun 376:504-8. 2008
    ..G alpha14 was co-expressed with T1r3, a sweet/umami taste receptor, but not with gustducin in CV. These results suggest the important roles of G alpha14 in sweet/umami taste signal transduction in the posterior region of the tongue...
  22. ncbi Mammalian Pins is a conformational switch that links NuMA to heterotrimeric G proteins
    Quansheng Du
    Center for Cell Signaling, Department of Microbiology, University of Virginia School of Medicine, Charlottesville, VA 22908, USA
    Cell 119:503-16. 2004
    ..We propose that a related switch mechanism might operate in asymmetric cell divisions in the fly and nematode...
  23. pmc The GAPs, GEFs, and GDIs of heterotrimeric G-protein alpha subunits
    David P Siderovski
    Department of Pharmacology, UNC Lineberger Comprehensive Cancer Center, and UNC Neuroscience Center, The University of North Carolina at Chapel Hill, CB 7365, 1106 M E Jones Building, Chapel Hill, NC 27599 7365 USA
    Int J Biol Sci 1:51-66. 2005
    ..Discovery of these novel GAP, GDI, and GEF activities have helped to illuminate a new role for Galpha subunit GDP/GTP cycling required for microtubule force generation and mitotic spindle function in chromosomal segregation...
  24. ncbi Molecular basis for interactions of G protein betagamma subunits with effectors
    C E Ford
    Institute for Neuroscience and Department of Molecular Pharmacology and Biological Chemistry, Northwestern University, Chicago, IL 60611, USA
    Science 280:1271-4. 1998
    ..This organization of interaction regions on Gbeta for different effectors and Galpha explains why subunit dissociation is crucial for signal transmission through Gbetagamma subunits...
  25. ncbi The role of Far1p in linking the heterotrimeric G protein to polarity establishment proteins during yeast mating
    A C Butty
    Swiss Institute for Experimental Cancer Research ISREC, Chemin des Boveresses 155, 1066 Epalinges VD, Switzerland
    Science 282:1511-6. 1998
    ..Thus, Far1p functions as an adaptor that recruits polarity establishment proteins to the site of extracellular signaling marked by Gbetagamma to polarize assembly of the cytoskeleton in a morphogenetic gradient...
  26. ncbi Established and emerging fluorescence-based assays for G-protein function: heterotrimeric G-protein alpha subunits and regulator of G-protein signaling (RGS) proteins
    Randall J Kimple
    Department of Pharmacology, Lineberger Comprehensive Cancer Center, and UNC Neuroscience Center, The University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 USA
    Comb Chem High Throughput Screen 6:399-407. 2003
    ..In this article, we focus on fluorescence-based methodologies to study heterotrimeric G-protein alpha subunit regulation in vitro. Furthermore, we consider the potential of such techniques in high-throughput screening and drug discovery...
  27. pmc Differential dissociation of G protein heterotrimers
    Gregory J Digby
    Department of Pharmacology and Toxicology, Medical College of Georgia, Augusta, GA 30809, USA
    J Physiol 586:3325-35. 2008
    ..Differential dissociation may thus provide a simple explanation for G alpha-specific activation of GIRK channels and other G betagamma-sensitive effectors...
  28. ncbi G-protein beta 3 subunit 825 CC genotype is associated with unexplained (functional) dyspepsia
    Gerald Holtmann
    Gastroenterology and Hepatology, University of Essen, Germany
    Gastroenterology 126:971-9. 2004
    ..We aimed to assess the association of specific G-protein beta 3 subunit genotypes with functional dyspepsia...
  29. pmc Inhibition of G-protein signaling by dominant gain-of-function mutations in Sst2p, a pheromone desensitization factor in Saccharomyces cerevisiae
    H G Dohlman
    Department of Pharmacology, Yale University School of Medicine, New Haven, Connecticut 06536 0812, USA
    Mol Cell Biol 15:3635-43. 1995
    ..These results point to the G protein alpha subunit as being the direct target of Sst2p action and underscore the importance of this novel desensitization factor in G-protein-mediated signaling...
  30. ncbi G protein regulation of ion channels and abscisic acid signaling in Arabidopsis guard cells
    X Q Wang
    Biology Department, Pennsylvania State University, 208 Mueller Laboratory, University Park, PA 16802 5301, USA
    Science 292:2070-2. 2001
    ..Manipulation of G protein status in guard cells may provide a mechanism for controlling plant water balance...
  31. ncbi G beta gamma mediates the interplay between tubulin dimers and microtubules in the modulation of Gq signaling
    Juliana S Popova
    Department of Physiology and Biophysics, College of Medicine, University of Illinois, Chicago, Illinois 60612 7342, USA
    J Biol Chem 278:34299-308. 2003
    ..Thus G protein-coupled receptors might evoke G alpha and G beta gamma to orchestrate regulation of phospholipase signaling by tubulin dimers and control of cell shape by microtubules...
  32. ncbi Suggestive association between the C825T polymorphism of the G-protein beta3 subunit gene (GNB3) and clinical improvement with antipsychotics in schizophrenia
    Daniel J Muller
    Neurogenetics Section, Centre for Addiction and Mental Health, Department of Psychiatry, University of Toronto, Toronto, ON, Canada
    Eur Neuropsychopharmacol 15:525-31. 2005
    ..8 to 7%. Our results further suggest that this effect is only attributable to Caucasians when compared to African-Americans. Moreover, our findings point to the role of intracellular mechanisms in antipsychotic response...
  33. pmc Mechanism of the regulation of type IB phosphoinositide 3OH-kinase byG-protein betagamma subunits
    Sonja Krugmann
    Signalling Programme, The Babraham Institute, Babraham, Cambridge CB2 4AT, U K
    Biochem J 362:725-31. 2002
    ..We conclude that Gbetagammas activate type IB PI3K by a mechanism other than translocation to the plasma membrane...
  34. ncbi Heterotrimeric G-proteins associate with microtubules during differentiation in PC12 pheochromocytoma cells
    Tulika Sarma
    Department of Physiology, College of Medicine, Chicago, Illinois 60612 7342, USA
    FASEB J 17:848-59. 2003
    ..Thus, diverse signals that promote neuronal differentiation and changes in cell morphology may use specific G-proteins to evoke cytoskeletal rearrangement...
  35. pmc Efficient signal transduction by a chimeric yeast-mammalian G protein alpha subunit Gpa1-Gsalpha covalently fused to the yeast receptor Ste2
    R Medici
    Istituto G Donegani, ENICHEM, 00016 Monterotondo Rome, Italy
    EMBO J 16:7241-9. 1997
    ..To confirm this conclusion, we show that two proteins interacting with each other (such as Snf1 and Snf4, or Ras and Raf), each of them fused either to the receptor or to the chimeric Galpha, allow efficient signal transduction...
  36. ncbi Ggamma13 colocalizes with gustducin in taste receptor cells and mediates IP3 responses to bitter denatonium
    L Huang
    Howard Hughes Medical Institute, Mount Sinai School of Medicine of New York University, Box 1677, One Gustave L Levy Place, New York, New York 10029, USA
    Nat Neurosci 2:1055-62. 1999
    ..We conclude that gustducin heterotrimers transduce responses to bitter and sweet compounds via alpha-gustducin's regulation of phosphodiesterase (PDE) and Gbetagamma's activation of phospholipase C (PLC)...
  37. ncbi Gbeta gamma isoforms selectively rescue plasma membrane localization and palmitoylation of mutant Galphas and Galphaq
    D S Evanko
    Department of Microbiology and Immunology and Kimmel Cancer Institute, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
    J Biol Chem 276:23945-53. 2001
    ....
  38. ncbi Leukemia-associated Rho guanine nucleotide exchange factor (LARG) links heterotrimeric G proteins of the G(12) family to Rho
    S Fukuhara
    Oral and Pharyngeal Cancer Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, 9000 Rockville Pike, Building 30, Room 211, Bethesda, MD 20892 4330, USA
    FEBS Lett 485:183-8. 2000
    ....
  39. ncbi Ric-8 controls Drosophila neural progenitor asymmetric division by regulating heterotrimeric G proteins
    Hongyan Wang
    Temasek Life Sciences Laboratory, 1 Research Link, National University of Singapore, Singapore 117604
    Nat Cell Biol 7:1091-8. 2005
    ..In addition, Gbetagamma acts to restrict Galphai (and GoLoco proteins) to the apical cortex, where Galphai (and Pins) can mediate asymmetric spindle geometry...
  40. ncbi Receptor-mediated activation of heterotrimeric G-proteins in living cells
    C Janetopoulos
    Department of Biological Chemistry, Johns Hopkins Medical Institutions, Baltimore, MD 21205, USA
    Science 291:2408-11. 2001
    ..Construction of similar energy-transfer pairs of mammalian G-proteins should enable direct in situ mechanistic studies and applications such as drug screening and identifying ligands of newly found G-protein-coupled receptors...
  41. ncbi Mammalian Ric-8A (synembryn) is a heterotrimeric Galpha protein guanine nucleotide exchange factor
    Gregory G Tall
    Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas 75390 9041, USA
    J Biol Chem 278:8356-62. 2003
    ..Ric-8A interacts with GDP-bound Galpha proteins, stimulates release of GDP, and forms a stable nucleotide-free transition state complex with the Galpha protein; this complex dissociates upon binding of GTP to Galpha...
  42. ncbi Regulation of G protein-initiated signal transduction in yeast: paradigms and principles
    H G Dohlman
    Department of Pharmacology, Boyer Center for Molecular Medicine, Yale University School of Medicine, New Haven, Connecticut 06536 0812, USA
    Annu Rev Biochem 70:703-54. 2001
    ....
  43. ncbi Receptor-mediated activation of heterotrimeric G-proteins: current structural insights
    Christopher A Johnston
    Department of Pharmacology, University of North Carolina at Chapel Hill, CB 7365, Chapel Hill, NC 27599 7365, USA
    Mol Pharmacol 72:219-30. 2007
    ..We highlight several recent results shedding new light on the structural changes in Galpha that may underlie GDP release...
  44. ncbi Visualization of a functional Galpha q-green fluorescent protein fusion in living cells. Association with the plasma membrane is disrupted by mutational activation and by elimination of palmitoylation sites, but not be activation mediated by receptors or
    T E Hughes
    Department of Ophthalmology Yale University School of Medicine, New Haven, Connecticut 06520-8026, USA
    J Biol Chem 276:4227-35. 2001
    ....
  45. ncbi Sst2 is a GTPase-activating protein for Gpa1: purification and characterization of a cognate RGS-Galpha protein pair in yeast
    D M Apanovitch
    Department of Pharmacology and Molecular Cardiobiology Program, Boyer Center for Molecular Medicine, Yale University School of Medicine, New Haven, Connecticut 06536, USA
    Biochemistry 37:4815-22. 1998
    ..These experiments represent the first biochemical characterization of Gpa1 and Sst2, and provide a molecular basis for their well-established biological roles in signaling and desensitization...
  46. pmc Galpha 12 activates Rho GTPase through tyrosine-phosphorylated leukemia-associated RhoGEF
    Nobuchika Suzuki
    Department of Pharmacology, University of Illinois, Chicago, IL 60612, USA
    Proc Natl Acad Sci U S A 100:733-8. 2003
    ..These results demonstrate the biochemical mechanism of Rho activation through Galpha12 and that the regulation of RhoGEFs by heterotrimeric G proteins G1213 is further modulated by tyrosine phosphorylation...
  47. pmc Heterotrimeric G protein signaling in the Arabidopsis unfolded protein response
    Shiyu Wang
    Biology Department and Huck Institutes of the Life Sciences, Pennsylvania State University, University Park, PA 16802, USA
    Proc Natl Acad Sci U S A 104:3817-22. 2007
    ....
  48. ncbi Purification and in vitro functional analysis of the Arabidopsis thaliana regulator of G-protein signaling-1
    Francis S Willard
    Department of Pharmacology, The University of North Carolina at Chapel Hill, 27599 7365, USA
    Methods Enzymol 389:320-38. 2004
    ..This article describes methods for the purification and in vitro functional analysis of the RGS box of AtRGS1...
  49. ncbi Homozygous 825T allele of the GNB3 protein influences the susceptibility of Japanese to dyspepsia
    Tomomitsu Tahara
    Department of Gastroenterology, Fujita Health University School of Medicine, 1 98 Dengakugakubo, Kutsukake cho, Toyoake, Aichi 470 1192, Japan
    Dig Dis Sci 53:642-6. 2008
    ..Our data suggest that the homozygous 825T allele of GNB3 protein is associated with dyspepsia in the H. pylori-negative Japanese population. The role of genetics in the development of dyspepsia needs further evaluation...
  50. ncbi Interaction of a G-protein beta-subunit with a conserved sequence in Ste20/PAK family protein kinases
    T Leeuw
    Eukaryotic Genetics Group, Biotechnology Research Institute, National Research Council of Canada, Montreal, Quebec
    Nature 391:191-5. 1998
    ....
  51. pmc Pheromone signalling in Saccharomyces cerevisiae requires the small GTP-binding protein Cdc42p and its activator CDC24
    Z S Zhao
    Institute of Molecular and Cell Biology, National University of Singapore, Kent Ridge
    Mol Cell Biol 15:5246-57. 1995
    ..The two-hybrid system revealed that Ste4p interacts with Cdc24p. We propose that Cdc42p plays a pivotal role both in polarization of the cytoskeleton and in pheromone signalling...
  52. pmc Saccharomyces cerevisiae cells execute a default pathway to select a mate in the absence of pheromone gradients
    R Dorer
    Department of Genetics, University of Washington, Seattle 98195 7360, USA
    J Cell Biol 131:845-61. 1995
    ..These observations reveal a molecular relationship between the mating and budding polarity pathways...
  53. pmc Heterotrimeric G proteins and apoptosis: intersecting signaling pathways leading to context dependent phenotypes
    Vijay Yanamadala
    Renal Division, Brigham and Women s Hospital and Harvard Medical School, Boston, MA 02115, USA
    Curr Mol Med 9:527-45. 2009
    ..Continued investigation in this field is essential for the identification of important targets for pharmacological intervention in numerous diseases...
  54. ncbi Role of cysteine 341 and arginine 348 of GLP-1 receptor in G-protein coupling
    Aljosa Bavec
    Institute of Biochemistry, Medical Faculty, University of Ljubljana, Vrazov trg 2, 1000, Ljubljana, Slovenia
    Mol Biol Rep 34:53-60. 2007
    ..Moreover, these two amino acids from the third intracellular loop might represent an important novel targets for covalent modification by downstream regulators in signaling through GLP-1 receptor...
  55. ncbi Structural determinants for GoLoco-induced inhibition of nucleotide release by Galpha subunits
    Randall J Kimple
    Department of Pharmacology, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    Nature 416:878-81. 2002
    ....
  56. pmc RGS14 is a novel Rap effector that preferentially regulates the GTPase activity of galphao
    S Traver
    Inserm U 528, Institut Curie Recherche, 26 Rue d Ulm, 75248 Paris Cedex 05, France
    Biochem J 350:19-29. 2000
    ....
  57. ncbi RGS12 and RGS14 GoLoco motifs are G alpha(i) interaction sites with guanine nucleotide dissociation inhibitor Activity
    R J Kimple
    Department of Pharmacology, Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, North Carolina 27599 7365, USA
    J Biol Chem 276:29275-81. 2001
    ..The presence of two, independent G alpha interaction sites suggests that RGS12 and RGS14 participate in a complex coordination of G-protein signaling beyond simple G alpha GAP activity...
  58. pmc G-protein signaling: back to the future
    C R McCudden
    Department of Pharmacology, Lineberger Comprehensive Cancer Center, and UNC Neuroscience Center, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, 27599 7365, USA
    Cell Mol Life Sci 62:551-77. 2005
    ..Here, we revisit classical heterotrimeric G-protein signaling and explore these new, non-canonical G-protein signaling pathways...
  59. ncbi Heterotrimeric G proteins and regulation of size asymmetry during cell division
    Yohanns Bellaiche
    Institut Curie, CNRS UMR 144, 26 Rue d Ulm, Paris, 75248 France
    Curr Opin Cell Biol 17:658-63. 2005
    ..Interestingly, recent work in mammalian cells suggests that heterotrimeric G proteins may control spindle positioning in higher organisms during symmetric and asymmetric cell divisions...
  60. ncbi Activation of heterotrimeric G-protein signaling by a ras-related protein. Implications for signal integration
    M J Cismowski
    OSI Pharmaceuticals, Tarrytown, New York 10591, The Department of Pharmacology, Medical University of South Carolina, Charleston, South Carolina 29425, USA
    J Biol Chem 275:23421-4. 2000
    ..AGS1 thus presents a unique mechanism for signal integration via heterotrimeric G-protein signaling pathways...
  61. ncbi Influence of cytosolic AGS3 on receptor--G protein coupling
    Hongzheng Ma
    Department of Biochemistry and Molecular Pharmacology, West Virginia University School of Medicine, Morgantown, West Virginia 26506 9142, USA
    Biochemistry 42:8085-93. 2003
    ..These findings suggest that AGS3 may downregulate heterotrimeric G protein signaling by interfering with receptor coupling...
  62. ncbi Receptor-dependent RhoA activation in G12/G13-deficient cells: genetic evidence for an involvement of Gq/G11
    Stephan Vogt
    Institute of Pharmacology, University of Heidelberg, Im Neuenheimer Feld 366, 69120 Heidelberg, Germany
    J Biol Chem 278:28743-9. 2003
    ..Our data clearly show that G12/G13 as well as Gq/G11 alone can couple GPCRs to the rapid activation of RhoA. Gq/G11-mediated RhoA activation occurs independently of phospholipase C-beta and appears to involve LARG...
  63. ncbi The roles of alpha IIb beta 3-mediated outside-in signal transduction, thromboxane A2, and adenosine diphosphate in collagen-induced platelet aggregation
    Moon J Cho
    Department of Microbiology and Molecular Cell Sciences, University of Memphis and the Division of Experimental Hematology, St Jude Children s Research Hospital, Memphis, TN, USA
    Blood 101:2646-51. 2003
    ..Furthermore, a high level of collagen can cause weak TxA2 and ADP-independent aggregation, but maximal aggregation induced by a high level of collagen requires TxA2 or secretion...
  64. ncbi RGSZ1, a Gz-selective RGS protein in brain. Structure, membrane association, regulation by Galphaz phosphorylation, and relationship to a Gz gtpase-activating protein subfamily
    J Wang
    Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, Texas 75235 9041, USA
    J Biol Chem 273:26014-25. 1998
    ..Phosphorylation of Galphaz by protein kinase C inhibited the GAP activity of RGSZ1 and other RGS proteins, providing a mechanism for potentiation of Gz signaling by protein kinase C...
  65. ncbi G protein beta3 subunit C825T polymorphism in primary IgA nephropathy
    Lise Thibaudin
    Department of Nephrology, Dialysis and Renal Transplantation, University North Hospital, Saint Etienne, France
    Kidney Int 66:322-8. 2004
    ..Hypertension is a strong independent risk factor for progression in IgA nephropathy (IgAN)...
  66. ncbi Plant heterotrimeric G protein function: insights from Arabidopsis and rice mutants
    Laetitia Perfus-Barbeoch
    Biology Department, Penn State University, University Park, Pennsylvania 16802 5301, USA
    Curr Opin Plant Biol 7:719-31. 2004
    ..Results and concepts emerging from recent phenotypic analyses of G-protein component mutants in Arabidopsis and rice are adding to our understanding of G-protein mechanisms and functions in higher plants...
  67. ncbi Nuclear localization of G protein beta 5 and regulator of G protein signaling 7 in neurons and brain
    J H Zhang
    Metabolic Diseases Branch and the Diabetes Branch, NIDDK, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 276:10284-9. 2001
    ..RGS protein complexes suggests a potential role in neurons communicating between classical heterotrimeric G protein subunits and/or their effectors at the plasma membrane and the cell nucleus...
  68. pmc Mating in Saccharomyces cerevisiae: the role of the pheromone signal transduction pathway in the chemotropic response to pheromone
    K Schrick
    Department of Genetics, University of Washington, Seattle 98195 7360, USA
    Genetics 147:19-32. 1997
    ....
  69. ncbi Activated G alpha q inhibits p110 alpha phosphatidylinositol 3-kinase and Akt
    Lisa M Ballou
    Research Service, Department of Veterans Affairs Medical Center, Northport, New York 11768, USA
    J Biol Chem 278:23472-9. 2003
    ..The inhibitory mechanism seems to be independent of phospholipase C activation and might involve an inhibitory interaction between Galphaq and p110alpha PI3K...
  70. ncbi Association analysis of a functional G protein beta3 subunit gene polymorphism (C825T) in mood disorders
    C N Lin
    Department of Psychiatry, Veterans General Hospital, Taipei, Taiwan, ROC
    Neuropsychobiology 44:118-21. 2001
    ..Further studies of the associations between other G protein subunits and mood disorder are needed to fully elaborate the involvement of this protein in mood disorders...
  71. ncbi Asymmetric cell division during animal development
    J A Knoblich
    Research Institute of Molecular Pathology IMP, Dr Bohr Gasse 7, A 1030 Vienna, Austria
    Nat Rev Mol Cell Biol 2:11-20. 2001
    ..The discovery of asymmetrically segregating proteins in vertebrates indicates that the results obtained in invertebrate model organisms might also apply to mammalian stem cells...
  72. ncbi Signal-dependent translocation of transducin, RGS9-1-Gbeta5L complex, and arrestin to detergent-resistant membrane rafts in photoreceptors
    K Saidas Nair
    Department of Molecular and Cellular Pharmacology, University of Miami School of Medicine, Miami, FL 33136, USA
    Curr Biol 12:421-5. 2002
    ..These findings could reveal a novel mechanism utilized by the cells for regulation of G protein-mediated signal transduction...
  73. ncbi Functional interaction between T2R taste receptors and G-protein alpha subunits expressed in taste receptor cells
    Takashi Ueda
    Department of Molecular Morphology, Graduate School of Medical Sciences, Nagoya City University, Nagoya 467 8601, Japan
    J Neurosci 23:7376-80. 2003
    ..The present Galpha16-based chimeras could be useful tools to analyze the functions of many orphan G-protein-coupled taste receptors...
  74. ncbi T-type calcium channel regulation by specific G-protein betagamma subunits
    Joshua T Wolfe
    Department of Pharmacology, University of Virginia, Charlottesville, Virginia 22908, USA
    Nature 424:209-13. 2003
    ..These studies identify the alpha1H channel as a new effector for G-protein betagamma subunits, and highlight the selective signalling roles available for particular betagamma combinations...
  75. ncbi RGS14 is a bifunctional regulator of Galphai/o activity that exists in multiple populations in brain
    S Hollinger
    Department of Pharmacology, Emory University School of Medicine, Atlanta, Georgia 30322, USA
    J Neurochem 79:941-9. 2001
    ..These findings highlight previously unknown biochemical properties of RGS14 in brain, and provide one of the first examples of an RGS protein that is a bifunctional regulator of Galpha actions...
  76. ncbi A zebrafish homologue of the chemokine receptor Cxcr4 is a germ-cell guidance receptor
    Holger Knaut
    Max Planck Institut fur Entwicklungsbiologie, Abteilung III Genetik, Spemannstrasse 35, 72076 Tubingen, Germany
    Nature 421:279-82. 2003
    ....
  77. ncbi Association between a G-protein beta 3 subunit gene polymorphism and the symptomatology and treatment responses of major depressive disorders
    H J Lee
    Department of Psychiatry, Korea University College of Medicine, Seoul, Korea
    Pharmacogenomics J 4:29-33. 2004
    ..It was also demonstrated that MDD patients bearing the T allele had a severe symptomatology and a better response to antidepressant treatment than patients without the T allele...
  78. pmc A mutant Arabidopsis heterotrimeric G-protein beta subunit affects leaf, flower, and fruit development
    K A Lease
    Division of Biological Sciences, University of Missouri Columbia, Columbia, Missouri 65211, USA
    Plant Cell 13:2631-41. 2001
    ..The finding that AGB1 is involved in the control of organ shape suggests that heterotrimeric G-protein signaling is a developmental regulator in Arabidopsis...
  79. pmc GPCRDB information system for G protein-coupled receptors
    Florence Horn
    Department of Cellular and Molecular Pharmacology, UCSF, San Francisco, California, USA
    Nucleic Acids Res 31:294-7. 2003
    ..The GPCRDB is updated automatically once every 4-5 months and is freely accessible at http://www.gpcr.org/7tm/...
  80. ncbi A unique fold of phospholipase C-beta mediates dimerization and interaction with G alpha q
    Alex U Singer
    Department of Pharmacology, The University of North Carolina at Chapel Hill, North Carolina 27599, USA
    Nat Struct Biol 9:32-6. 2002
    ..Effector dimerization, as highlighted by PLC-betas, provides a viable mechanism for regulating signaling cascades linked to heterotrimeric G proteins...
  81. ncbi Comparative inter-strain sequence analysis of the putative regulatory region of murine psychostimulant-regulated gene GNB1 (G protein beta 1 subunit gene)
    Nobue Kitanaka
    Molecular Neurobiology Branch, Intramural Research Program, National Institute on Drug Abuse, National Institutes of Health, Department of Health and Human Services, Baltimore, MD 21224, USA
    DNA Seq 14:257-63. 2003
    ....
  82. ncbi Regulation of mGlu4 metabotropic glutamate receptor signaling by type-2 G-protein coupled receptor kinase (GRK2)
    L Iacovelli
    Dept of Human Physiology and Pharmacology, University of Rome La Sapienza, Italy
    Mol Pharmacol 65:1103-10. 2004
    ..We speculate that GRK2 acts as a "switch molecule" by inhibiting the mGlu4 receptor-mediated stimulation of MAPK and therefore directing the signal propagation toward the inhibition of adenylyl cyclase...
  83. ncbi Modulation of cell proliferation by heterotrimeric G protein in Arabidopsis
    H Ullah
    Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    Science 292:2066-9. 2001
    ..Results from loss of function and ectopic expression and activation of GPA1 indicate that this subunit is a positive modulator of cell division in plants...
  84. pmc Multiple sweet receptors and transduction pathways revealed in knockout mice by temperature dependence and gurmarin sensitivity
    Tadahiro Ohkuri
    Graduate School of Dental Science, Kyushu University, Fukuoka, 812 8582, Japan
    Am J Physiol Regul Integr Comp Physiol 296:R960-71. 2009
    ....
  85. pmc Sst2, a negative regulator of pheromone signaling in the yeast Saccharomyces cerevisiae: expression, localization, and genetic interaction and physical association with Gpa1 (the G-protein alpha subunit)
    H G Dohlman
    Department of Pharmacology, Boyer Center for Molecular Medicine, Yale University School of Medicine, New Haven, Connecticut 06536 0812, USA
    Mol Cell Biol 16:5194-209. 1996
    ..These results demonstrate that Sst2 and Gpa1 interact physically and suggest that Sst2 is a direct negative regulator of Gpa1...
  86. ncbi Characterizing the interactions between the two subunits of the p101/p110gamma phosphoinositide 3-kinase and their role in the activation of this enzyme by G beta gamma subunits
    S Krugmann
    Signalling Programme, The Babraham Institute, Babraham, Cambridge CB2 4AT, United Kingdom
    J Biol Chem 274:17152-8. 1999
    ..While modifications of the N terminus of p110gamma could modulate its intrinsic catalytic activity, binding to the N-terminal region of p101 was found to be indispensable for activation of heterodimers with Gbetagamma...
  87. ncbi Activation of phospholipase C-epsilon by heterotrimeric G protein betagamma-subunits
    M R Wing
    Department of Pharmacology, Program in Neurobiology, Lineberger Cancer Center, University of North Carolina School of Medicine, Chapel Hill, North Carolina 27599, USA
    J Biol Chem 276:48257-61. 2001
    ..Taken together, these results reveal the presence of additional functional domains in PLC-epsilon and add a new level of complexity in the regulation of this novel enzyme by heterotrimeric G proteins...
  88. pmc Assembly and trafficking of heterotrimeric G proteins
    Yannick Marrari
    Department of Biochemistry and Molecular Biology, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
    Biochemistry 46:7665-77. 2007
    ..Understanding G protein assembly and trafficking will lead to a greater understanding of novel ways that cells regulate G protein signaling...
  89. ncbi G-protein beta3 subunit C825T polymorphism tends to be associated with seasonal variation in young male college students
    Heon Jeong Lee
    Department of Psychiatry, Anam Hospital, Korea University College of Medicine, Anam Dong, Seongbuk gu, Seoul 136 705, Korea
    Neuropsychobiology 52:135-9. 2005
    ..These results suggest that GNB3 C825T polymorphism tends to be related to seasonal variation in mood and behavior in the normal population...
  90. ncbi G protein beta3 polymorphism and triptan response in cluster headache
    M Schurks
    Department of Neurology, University Hospital Essen, University of Duisburg Essen, Essen, Germany
    Clin Pharmacol Ther 82:396-401. 2007
    ..e., drugs not directly affecting G proteins. We conclude that pain relief by triptans is significantly modulated by a common genetic GNB3 variant...
  91. ncbi Identification of the Gbeta5-RGS7 protein complex in the retina
    J L Cabrera
    Department of Molecular and Cellular Pharmacology, University of Miami School of Medicine, Florida 33136, USA
    Biochem Biophys Res Commun 249:898-902. 1998
    ..G beta 5 was not detected in the outer segments of photoreceptor cells, suggesting that the cytosolic G beta 5-RGS7 complex is not directly involved in phototransduction...
  92. ncbi Immunohistochemical localization of G protein beta1, beta2, beta3, beta4, beta5, and gamma3 subunits in the adult rat brain
    J J Liang
    CNS Disorders, Wyeth Ayerst Research, Princeton, New Jersey 08543 8000, USA
    J Neurochem 71:345-55. 1998
    ..In conclusion, despite the widespread overlapping distributions of Gbeta1-beta5 with Ggamma3, specific dimeric associations in situ were observed within discrete brain regions...
  93. ncbi G proteins Go green: a plant G protein signaling FAQ sheet
    Sarah M Assmann
    Biology Department, Pennsylvania State University, 208 Mueller Laboratory, University Park, PA 16802, USA
    Science 310:71-3. 2005
    ..Pathways in Science's Signal Transduction Knowledge Environment Connections Maps database provide details about the emerging roles of G proteins in several cellular processes of plants...
  94. ncbi G-protein beta3 subunit variant C825T is a risk factor for hypertension in Japanese females--a prospective cohort study over 5 years
    Y Suwazono
    Department of Occupational and Environmental Medicine, Graduate School of Medicine, Chiba University, 1 8 1 Inohana, Chuo Ku, Chiba 260 8670, Japan
    Ann Hum Genet 70:767-77. 2006
    ....
  95. ncbi Regulation of membrane and subunit interactions by N-myristoylation of a G protein alpha subunit in yeast
    J Song
    Department of Pharmacology, Boyer Center for Molecular Medicine, Yale University School of Medicine, New Haven, Connecticut 06536 0812, USA
    J Biol Chem 271:20273-83. 1996
    ..These data suggest that myristoylation is required for specific targeting of Gpa1p to the plasma membrane, where it is needed to interact with the receptor and to regulate the release of Gbetagamma...
  96. ncbi Receptor-independent activators of heterotrimeric G-protein signaling pathways
    A Takesono
    Department of Pharmacology, Medical University of South Carolina, Charleston, South Carolina 29425, USA
    J Biol Chem 274:33202-5. 1999
    ..AGS2 and AGS3 may also serve as novel binding partners for Galpha and Gbetagamma that allow the subunits to subserve functions that do not require initial heterotrimer formation...
  97. ncbi Heterotrimeric G proteins regulate reproductive trait plasticity in response to water availability
    Sarah E Nilson
    Biology Department, Pennsylvania State University, 208 Mueller Laboratory, University Park, PA 16802 5301, USA
    New Phytol 185:734-46. 2010
    ....
  98. ncbi The thrombin receptor, PAR-1, causes transformation by activation of Rho-mediated signaling pathways
    C B Martin
    Department of Pharmacology and Lineberger Comprehensive Cancer Center, University of North Carolina School of Medicine, Chapel Hill, North Carolina NC 27599-7295, USA
    Oncogene 20:1953-63. 2001
    ..Taken together, these observations suggest that PAR-1 growth transformation is mediated, in part, by activation of RhoA...
  99. ncbi The G betagamma dimer as a novel source of selectivity in G-protein signaling: GGL-ing at convention
    Miller B Jones
    Department of Pharmacology, School of Medicine, The University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 7365, USA
    Mol Interv 4:200-14. 2004
    ....
  100. ncbi Inhibition of GDP/GTP exchange on G alpha subunits by proteins containing G-protein regulatory motifs
    M Natochin
    Department of Physiology and Biophysics, University of Iowa College of Medicine, Iowa City, Iowa 52242, USA
    Biochemistry 40:5322-8. 2001
    ..Our results suggest that GPR proteins are potent GDP dissociation inhibitors for Gialpha-like Galpha subunits in vitro, and in this capacity they may inhibit GPCR/Gi protein signaling in vivo...
  101. ncbi RGS-containing RhoGEFs: the missing link between transforming G proteins and Rho?
    S Fukuhara
    Oral and Pharyngeal Cancer Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD 20892 4340, USA
    Oncogene 20:1661-8. 2001

Research Grants63

  1. Coordinate Regulation Of Nuclear DGK-theta and PLD
    DANIEL RABEN; Fiscal Year: 2009
    ..We believe that an understanding of nuclear lipid signaling is key to our ability to intervene in events such as wound repair and tissue remodeling. ..
  2. BIOPHYSICS OF RECEPTOR/G-PROTEIN INTERACTIONS
    Oleg G Kisselev; Fiscal Year: 2011
    ....
  3. TRANSMEMBRANE SIGNALLING IN GENETIC OBESITY
    Kathryn LaNoue; Fiscal Year: 1993
    ..Gi is one of a closely related family of heterotrimeric GTP binding proteins which function as intermediates in transmembrane signalling...
  4. HETEROTRIMERIC G PROTEINS AND EXOCYTOSIS
    BHANU JENA; Fiscal Year: 1999
    ..Understanding the role of the secretory granule membrane-associated G-proteins in exocytosis will help us understand better this fundamental cellular process which when impaired leads to a number of diseases. ..
  5. PHOSPHOLIPASES AND INSULIN SECRETION
    Bryan Wolf; Fiscal Year: 1999
    ..In the second approach, we will determine the identity and role of the three proteins, p24, p50, and p95, that are coupled to Galphai following glucose stimulation of beta-cells. ..
  6. REGULATION OF SIGNAL TRANSDUCTION IN DICTYOSTELIUM
    CHARLES SAXE; Fiscal Year: 2000
    ..Answers to questions addressed here should bear on broader issues of how multiple receptors, for specific signals, are used to regulate cell behavior and development. ..
  7. PSGR as a Molecular Target in Human Prostate Cancer
    Mingyao Liu; Fiscal Year: 2005
    ..abstract_text> ..
  8. REGULATION OF ION CHANNELS BY SPECIFIC G PROTEINS
    MALGORZATA WILK BLASZCZAK; Fiscal Year: 1999
    ..Thus this project is relevant for understanding and treating diseases of both the central nervous system and the peripheral tissues. ..
  9. Biophsyics of Receptor/G-protein Interactions
    OLEG KISSELEV; Fiscal Year: 2007
    ..abstract_text> ..
  10. Real-Time Fluorescence Assay:RGS Domain GAP Activit(RMI)
    DAVID SIDEROVSKI; Fiscal Year: 2008
    ..unreadable]PERFORMANCE SITE ========================================Section End=========================================== ..
  11. G-protein signal coordination by RGS12
    DAVID SIDEROVSKI; Fiscal Year: 2009
    ..We wish to study one RGS protein, RGS12, that interferes with the actions of the neurotransmitter GABA in neurons that control pain processing. This work should lead to the discovery of new pain-controlling drugs. ..
  12. Mechanistic studies of a novel G-alpha nucleotide cycle
    DAVID SIDEROVSKI; Fiscal Year: 2009
    ....
  13. Acquiring robotic support for mass spectrometry core
    CRAIG MALBON; Fiscal Year: 2003
    ..The institution has provided resources to enable the acquisition of two new mass spectrometers and this proposal builds on the investment. ..
  14. ALPHA 2 ADRENERGIC CONTROL IN IRRITABLE BOWEL SYNDROME
    Michael Camilleri; Fiscal Year: 2008
    ..Significance: This project will characterize pharmacogenetic mechanisms determining the response to clonidine through studies of candidate genes that control alpha2-adrenoreceptor function and norepinephrine transport in IBS. ..
  15. Mechanisms of ATP Release from RBC's
    Randy Sprague; Fiscal Year: 2007
    ..abstract_text> ..
  16. Cannabinoid Mechanisms in Human Gastrointestinal Motor and Sensory Functions
    MICHAEL L CAMILLERI; Fiscal Year: 2010
    ..These actions may be relevant to develop new treatments for IBS. The studies will also provide further understanding of the potential of medications like medical marijuana on stomach function that may be relevant to appetite control. ..
  17. Atherosclerosis And Peripheral Apoliprotein E Synthesis
    CRAIG MALBON; Fiscal Year: 2007
    ..These studies will provide new mechanistic information about actions of apoE on cholesterol metabolism and atherosclerosis. ..
  18. RENOVATIONS FOR CAGEWASH SUPPORT SERVICE
    Thomas Zimmerman; Fiscal Year: 2005
    ....
  19. Role of Erythrocytes in Circulatory Control
    Randy Sprague; Fiscal Year: 2004
    ..abstract_text> ..
  20. REGULATION OF HORMONE-SENSITIVE EFFECTOR SYSTEMS
    Craig C Malbon; Fiscal Year: 2010
    ..Dvls, as multivalent dynamic scaffolds, are high-value likely targets for new therapies. ..
  21. ALPHA-2 ADRENERGIC CONTROL OF COLONIC FUNCTION IN IBS
    Michael Camilleri; Fiscal Year: 2007
    ..abstract_text> ..
  22. CELL CYCLE ARREST AND SIGNAL TRANSDUCTION IN YEAST
    ELAINE ELION; Fiscal Year: 2009
    ..abstract_text> ..
  23. Adrenergic and Purinergic Regulation of Target Cells
    Paul A Insel; Fiscal Year: 2010
    ....
  24. Agonist Activity at G Protein Coupled Receptors
    FRED EHLERT; Fiscal Year: 2008
    ..Our proposed investigation of Galpha15 and Galpha16 is highly significant with regard to the widespread use of this G protein as a transducer for both orphan and known receptors. ..
  25. Function of calcium exchangers in vetebrate pigmentation
    KEITH CHI CHENG; Fiscal Year: 2010
    ....
  26. Anti-inflammatory Natural Product Mode of Action Studies
    CRAIG CREWS; Fiscal Year: 2008
    ..abstract_text> ..
  27. Sensitization in Trigeminal Neurons: A Role in Migraine?
    JOYCE HURLEY; Fiscal Year: 2008
    ..abstract_text> ..
  28. Natural Product Model of Action Studies
    CRAIG CREWS; Fiscal Year: 2008
    ..abstract_text> ..
  29. CRCNS:Comput./Exp.Study:Hypothal.-Pituitary Interaction
    Richard Bertram; Fiscal Year: 2008
    ..It will then lead to studies of effects of drugs of abuse on brain-pituitary function. [unreadable] [unreadable]..
  30. Prescription opioid agonists: tolerance and efficacy
    BYRON YOBURN; Fiscal Year: 2008
    ..Overall, the results of this project will provide new and important information on the pharmacodynamics and mechanism of action of a group of opioid analgesics that are potential drugs of abuse. [unreadable] [unreadable] [unreadable]..
  31. Trafficking and signaling of opioid receptor subtypes and heterodimers
    Jennifer L Whistler; Fiscal Year: 2010
    ..Thus targeting these subtypes or heterodimers could provide improved therapeutic utility. ..
  32. EUKARYOTIC GENE REGULATION AS STUDIED IN YEAST
    Mark Ptashne; Fiscal Year: 2009
    ..In particular we are testing the idea that this protein, a component of the large complex called the Mediator, is rapidly degraded in cells, and that this instability facilitates activation of transcription ..
  33. Interactions Between p90 Ribosomal S6 Kinase and Protein Kinase A
    Tarun B Patel; Fiscal Year: 2010
    ..These novel insights may then permit the development of specific interventions that regulate certain functions of both these kinases. ..
  34. GI PEPTIDE SIGNALING THROUGH TYROSINE PHOSPHORYLATION
    JUAN ENRIQUE ROZENGURT; Fiscal Year: 2010
    ....
  35. Genetics of Chronic Kidney Disease
    Stephen T Turner; Fiscal Year: 2010
    ..abstract_text> ..
  36. Modulation of Cellular Signaling by Sprouty Proteins
    Tarun Patel; Fiscal Year: 2008
    ..unreadable] [unreadable]..
  37. The International Symposium on Regulatory Peptides
    JUAN ROZENGURT; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  38. Family Blood Pressure Program (GenNet Network)
    Alan Weder; Fiscal Year: 2007
    ..unreadable] (End of Abstract) [unreadable] [unreadable]..
  39. Rho-Dependent COX-2 Expression in Intestinal Cells
    Lee Slice; Fiscal Year: 2007
    ..3) Define the role of Rho and of Protein Kinases acting upstream and downstream of Rho in COX-2 expression in intestinal epithelial cells. 4) Characterize COX-2 promoter elements that are responsive to Rho. ..
  40. Beta-Adrenergic Receptors of S49 Lymphoma Cells
    Paul Insel; Fiscal Year: 2005
    ..Moreover, the results should prove applicable to the many other hormone and neurotransmitter receptors that link to G proteins. ..
  41. A HISTOLOGIC SCREEN FOR CELL DIFFERENTIATION MUTANTS
    Keith Cheng; Fiscal Year: 2005
    ..This project will contribute to an elucidation of both organ-specific and general pathways of cell differentiation in vertebrates. ..
  42. REGULATION OF A CARDIAC SPECIFIC EFFECTOR
    Tarun Patel; Fiscal Year: 2005
    ..Thus the elucidation of the role of PAM in modulating AC activity in intact cells and the mechanisms of its action will provide new insights into how AC activity can be regulated. ..
  43. Signaling Diversity Among Gqa Family Members
    JOHN HEPLER; Fiscal Year: 2004
    ..Information gained from these studies will help us to better understand G proteins as therapeutic targets. ..
  44. QUANTITATIVE GENETICS OF OPIATE ADDICTION
    Wade Berrettini; Fiscal Year: 2004
    ..This may enhance our knowledge of genetic susceptibility to opioid dependence in human populations. ..
  45. Protein Kinase in Opioid Receptor Regulation & Tolerance
    BYRON YOBURN; Fiscal Year: 2003
    ..As such, these results may provide important insights for developing strategies to treat opioid drug abuse and pain. ..
  46. Molecular Basis of D1 and D2 Priming
    Alexia Pollack; Fiscal Year: 2003
    ..Elucidation of this mechanism could lead to treatment strategies aimed to prevent these dyskinesias, which would, in turn, lengthen the time that dopamine replacement therapy is effective to treat patients with PD. ..
  47. SIGNAL TRANDUCTION IN CELL PROLIFERATION
    David Manning; Fiscal Year: 2002
    ..We will also identify genes activated by LPA and S1P, and the subset whose activation is dependent on NF-kappaB. ..
  48. Inhibitors of PAR1-G13 Interface for Arthersclerosis
    Annette Gilchrist; Fiscal Year: 2002
    ..Hundreds of billions of dollars are spent each year treating heart disease. In 1998, it was estimated that sales of cardiovascular drugs alone were $37 billion. ..
  49. Genetics of Anorexia Nervosa
    Wade Berrettini; Fiscal Year: 2005
    ..The diagnostic and genetic data and lymphoblastoid cell lines (derived from blood samples) will become part of a national archival resource for genetic studies of AN through the NIMH Genetics Initiative. ..
  50. Orexinergic Pathways in Central Autonomic Control
    WILLIS SAMSON; Fiscal Year: 2005
    ..abstract_text> ..
  51. uPA RECEPTOR SIGNALING AND CELL MIGRATION
    Mark Davies; Fiscal Year: 2005
    ..It is anticipated that data generated from these studies will provide valuable insights for the development of therapeutic intervention to address clinical relevant problems of vessel restenosis. (End of Abstract) ..
  52. Role of Endocannabinoid Signaling in Alcoholism
    BALAPAL BASAVARAJAPPA; Fiscal Year: 2007
    ..The better understanding of these mechanisms will yield new drug therapies with potential in treatment of alcoholism. ..
  53. GASP and Trafficking of the Dopamine Receptors
    Jennifer Whistler; Fiscal Year: 2007
    ..These studies will determine whether GPCR sorting can be manipulated both in vitro and in vivo. This may provide a new spectrum of therapeutic targets for the treatment of schizophrenia and depression. [unreadable] [unreadable]..
  54. G PROTEIN BETA GAMMA SIGNALING SYSTEMS IN VIVO
    JANET ROBISHAW; Fiscal Year: 2007
    ..Since many diseases (e.g. cancer, heart disease) are associated with re-induction of embryonic genes, this information will eventually lead to the better diagnosis and more selective therapeutic treatments. ..
  55. A COLLABORATIVE GENOMIC STUDY OF BIPOLAR DISORDER
    Wade Berrettini; Fiscal Year: 2007
    ..Analysis of the existing sib pair families plus this large set of cases and controls should permit the confirmation of several vulnerability genes during this grant period. ..
  56. HYPGENE-GENETICS FITNESS OBESITY & RISK OF HYPERTENSION
    Tuomo Rankinen; Fiscal Year: 2007
    ..These data could ultimately lead to more efficient use of physical activity and body weight control in the primary and secondary prevention of hypertension. ..
  57. FGF receptor signaling specificity in C.elegans
    MICHAEL KOELLE; Fiscal Year: 2007
    ..In this way, we will gain a clearer understanding of how specific responses are elicited by the activation of FGF receptors. ..
  58. HYPERTENSION AMONG AFRICAN-AMERICANS
    JEFFERY YOUNG; Fiscal Year: 2006
    ..This research will build a foundation for a successful career as an independent investigator. ..
  59. Anthipertensive PharmacoGenomics
    Stephen Turner; Fiscal Year: 2006
    ..abstract_text> ..
  60. RECEPTOR COUPLING TO G12 AND G13
    David Manning; Fiscal Year: 2006
    ..abstract_text> ..
  61. MOLECULAR BASIS OF RECEPTOR CYCLASE COUPLING
    Henry Bourne; Fiscal Year: 2006
    ..unreadable] [unreadable]..
  62. ALPHA1-ADRENERGIC SIGNALING PATHWAYS IN HEART
    JANET ROBISHAW; Fiscal Year: 2002
    ..These studies are critical for the design of new therapeutic strategies aimed at enhancing or counteracting alpha/1-AR subtype specific effects in the heart. ..