ribonucleotides

Summary

Summary: Nucleotides in which the purine or pyrimidine base is combined with ribose. (Dorland, 28th ed)

Top Publications

  1. pmc Role of AMP-activated protein kinase in mechanism of metformin action
    G Zhou
    Department of Molecular Endocrinology, Merck Research Laboratories, Rahway, New Jersey 07065, USA
    J Clin Invest 108:1167-74. 2001
  2. pmc RNase H2-initiated ribonucleotide excision repair
    Justin L Sparks
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St Louis, MO 63110, USA
    Mol Cell 47:980-6. 2012
  3. pmc Enzymatic removal of ribonucleotides from DNA is essential for mammalian genome integrity and development
    Martin A M Reijns
    Medical Research Council Human Genetics Unit, MRC Institute of Genetics and Molecular Medicine, University of Edinburgh, Edinburgh EH4 2XU, UK
    Cell 149:1008-22. 2012
  4. pmc Abundant ribonucleotide incorporation into DNA by yeast replicative polymerases
    Stephanie A Nick McElhinny
    Laboratory of Molecular Genetics and Laboratory of Structural Biology, Department of Health and Human Services, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA
    Proc Natl Acad Sci U S A 107:4949-54. 2010
  5. ncbi AMP-activated protein kinase suppresses protein synthesis in rat skeletal muscle through down-regulated mammalian target of rapamycin (mTOR) signaling
    Douglas R Bolster
    Department of Cellular and Molecular Physiology, The Pennsylvania State University College of Medicine, Hershey, Pennsylvania 17033, USA
    J Biol Chem 277:23977-80. 2002
  6. pmc Mammalian RNase H2 removes ribonucleotides from DNA to maintain genome integrity
    Bjoern Hiller
    Institute for Immunology, Medical Faculty Carl Gustav Carus, University of Technology Dresden, 01307 Dresden, Germany
    J Exp Med 209:1419-26. 2012
  7. ncbi Activation of AMP-activated protein kinase increases mitochondrial enzymes in skeletal muscle
    W W Winder
    Department of Zoology, Brigham Young University, Provo, Utah 84602, USA
    J Appl Physiol (1985) 88:2219-26. 2000
  8. pmc DNA polymerase beta ribonucleotide discrimination: insertion, misinsertion, extension, and coding
    Nisha A Cavanaugh
    Laboratory of Structural Biology, NIEHS, National Institutes of Health, Research Triangle Park, North Carolina 27709 2233, USA
    J Biol Chem 285:24457-65. 2010
  9. ncbi 5-Aminoimidazole-4-carboxamide-1-beta-D-ribofuranoside inhibits cancer cell proliferation in vitro and in vivo via AMP-activated protein kinase
    Ramandeep Rattan
    Department of Pediatrics, Medical University of South Carolina, Charleston, South Carolina 29425, USA
    J Biol Chem 280:39582-93. 2005
  10. ncbi Synthesis of activated pyrimidine ribonucleotides in prebiotically plausible conditions
    Matthew W Powner
    School of Chemistry, The University of Manchester, Oxford Road, Manchester M13 9PL, UK
    Nature 459:239-42. 2009

Detail Information

Publications307 found, 100 shown here

  1. pmc Role of AMP-activated protein kinase in mechanism of metformin action
    G Zhou
    Department of Molecular Endocrinology, Merck Research Laboratories, Rahway, New Jersey 07065, USA
    J Clin Invest 108:1167-74. 2001
    ..Activation of AMPK provides a unified explanation for the pleiotropic beneficial effects of this drug; these results also suggest that alternative means of modulating AMPK should be useful for the treatment of metabolic disorders...
  2. pmc RNase H2-initiated ribonucleotide excision repair
    Justin L Sparks
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St Louis, MO 63110, USA
    Mol Cell 47:980-6. 2012
    b>Ribonucleotides are incorporated into DNA by the replicative DNA polymerases at frequencies of about 2 per kb, which makes them by far the most abundant form of potential DNA damage in the cell...
  3. pmc Enzymatic removal of ribonucleotides from DNA is essential for mammalian genome integrity and development
    Martin A M Reijns
    Medical Research Council Human Genetics Unit, MRC Institute of Genetics and Molecular Medicine, University of Edinburgh, Edinburgh EH4 2XU, UK
    Cell 149:1008-22. 2012
    The presence of ribonucleotides in genomic DNA is undesirable given their increased susceptibility to hydrolysis. Ribonuclease (RNase) H enzymes that recognize and process such embedded ribonucleotides are present in all domains of life...
  4. pmc Abundant ribonucleotide incorporation into DNA by yeast replicative polymerases
    Stephanie A Nick McElhinny
    Laboratory of Molecular Genetics and Laboratory of Structural Biology, Department of Health and Human Services, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA
    Proc Natl Acad Sci U S A 107:4949-54. 2010
    ....
  5. ncbi AMP-activated protein kinase suppresses protein synthesis in rat skeletal muscle through down-regulated mammalian target of rapamycin (mTOR) signaling
    Douglas R Bolster
    Department of Cellular and Molecular Physiology, The Pennsylvania State University College of Medicine, Hershey, Pennsylvania 17033, USA
    J Biol Chem 277:23977-80. 2002
    ..This is the first investigation to demonstrate changes in translation initiation and skeletal muscle protein synthesis in response to AMPK activation...
  6. pmc Mammalian RNase H2 removes ribonucleotides from DNA to maintain genome integrity
    Bjoern Hiller
    Institute for Immunology, Medical Faculty Carl Gustav Carus, University of Technology Dresden, 01307 Dresden, Germany
    J Exp Med 209:1419-26. 2012
    ..RNase H2-deficient cells featured an increased genomic ribonucleotide load, suggesting that unrepaired ribonucleotides trigger the DNA damage response in these cells...
  7. ncbi Activation of AMP-activated protein kinase increases mitochondrial enzymes in skeletal muscle
    W W Winder
    Department of Zoology, Brigham Young University, Provo, Utah 84602, USA
    J Appl Physiol (1985) 88:2219-26. 2000
    ..These results suggest that chronic AMPK activation may mediate the effects of muscle contraction on some, but not all, biochemical adaptations of muscle to endurance exercise training...
  8. pmc DNA polymerase beta ribonucleotide discrimination: insertion, misinsertion, extension, and coding
    Nisha A Cavanaugh
    Laboratory of Structural Biology, NIEHS, National Institutes of Health, Research Triangle Park, North Carolina 27709 2233, USA
    J Biol Chem 285:24457-65. 2010
    ..Although DNA polymerases discriminate against ribonucleotides, many therapeutic nucleotide analogs that target polymerases have sugar modifications, and their efficacy ..
  9. ncbi 5-Aminoimidazole-4-carboxamide-1-beta-D-ribofuranoside inhibits cancer cell proliferation in vitro and in vivo via AMP-activated protein kinase
    Ramandeep Rattan
    Department of Pediatrics, Medical University of South Carolina, Charleston, South Carolina 29425, USA
    J Biol Chem 280:39582-93. 2005
    ..Altogether, these results indicate that AICAR can be utilized as a therapeutic drug to inhibit cancer, and AMPK can be a potential target for treatment of various cancers independent of the functional tumor suppressor gene, LKB...
  10. ncbi Synthesis of activated pyrimidine ribonucleotides in prebiotically plausible conditions
    Matthew W Powner
    School of Chemistry, The University of Manchester, Oxford Road, Manchester M13 9PL, UK
    Nature 459:239-42. 2009
    ..In particular, although there has been some success demonstrating that 'activated' ribonucleotides can polymerize to form RNA, it is far from obvious how such ribonucleotides could have formed from their ..
  11. pmc Lack of sugar discrimination by human Pol mu requires a single glycine residue
    Jose F Ruiz
    Centro de Biologia Molecular Severo Ochoa CSIC UAM, Campus de la Universidad Autonoma de Madrid, Cantoblanco, 28049 Madrid, Spain
    Nucleic Acids Res 31:4441-9. 2003
    ..is demonstrated by its ability to add rNTPs to both DNA and RNA primer strands, and to insert both deoxy- and ribonucleotides on growing nucleic acid chains...
  12. pmc Mutagenic processing of ribonucleotides in DNA by yeast topoisomerase I
    Nayun Kim
    Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, NC 27710, USA
    Science 332:1561-4. 2011
    ..RNase H2 is specialized to remove single ribonucleotides [ribonucleoside monophosphates (rNMPs)] from duplex DNA, and its absence in budding yeast has been associated ..
  13. ncbi Regulation of mammalian ribonucleotide reduction and dNTP pools after DNA damage and in resting cells
    Pelle Håkansson
    Department of Medical Biochemistry and Biophysics, Umea University, SE 90187 Umea, Sweden
    J Biol Chem 281:7834-41. 2006
    ....
  14. pmc Ribonucleotides misincorporated into DNA act as strand-discrimination signals in eukaryotic mismatch repair
    Medini Manohar Ghodgaonkar
    Institute of Molecular Cancer Research of the University of Zurich and ETH Zurich, Winterthurerstrasse 190, 8057 Zurich, Switzerland
    Mol Cell 50:323-32. 2013
    ..We therefore propose that ribonucleotides misincoporated during DNA replication serve as physiological markers of the nascent DNA strand.
  15. pmc Critical amino acids in Escherichia coli UmuC responsible for sugar discrimination and base-substitution fidelity
    Alexandra Vaisman
    Laboratory of Genomic Integrity, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892 3371, USA
    Nucleic Acids Res 40:6144-57. 2012
    ..Remarkably, under these conditions, wild-type pol V Mut efficiently incorporates ribonucleotides into DNA...
  16. pmc Genome instability due to ribonucleotide incorporation into DNA
    Stephanie A Nick McElhinny
    Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, National Institutes of Health, Department of Health and Human Services, Research Triangle Park, North Carolina, USA
    Nat Chem Biol 6:774-81. 2010
    ..However, ribonucleotide exclusion during DNA synthesis in vitro is imperfect. To determine whether ribonucleotides are incorporated during DNA replication in vivo, we substituted leucine or glycine for an active-site ..
  17. pmc Ribonucleotides are signals for mismatch repair of leading-strand replication errors
    Scott A Lujan
    Laboratory of Molecular Genetics and Laboratory of Structural Biology, National Institute of Environmental Health Sciences, NIH, DHHS, Research Triangle Park, NC 27709, USA
    Mol Cell 50:437-43. 2013
    ..mismatch repair in Saccharomyces cerevisiae is reduced by inactivating RNase H2, which nicks DNA containing ribonucleotides incorporated during replication...
  18. pmc Polymerase mu is a DNA-directed DNA/RNA polymerase
    Stephanie A Nick McElhinny
    Department of Biochemistry and Biophysics and Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    Mol Cell Biol 23:2309-15. 2003
    DNA polymerases are defined as such because they use deoxynucleotides instead of ribonucleotides with high specificity...
  19. ncbi Biased incorporation of ribonucleotides on the mitochondrial L-strand accounts for apparent strand-asymmetric DNA replication
    Ming Yao Yang
    MRC Dunn Human Nutrition Unit, Hills Road, CB2 2XY, Cambridge, United Kingdom
    Cell 111:495-505. 2002
    ..their length, but contain widespread regions of RNA:DNA hybrid, as a result of the incorporation of ribonucleotides on the light strand which are subsequently converted to DNA...
  20. ncbi AMP-activated protein kinase in metabolic control and insulin signaling
    Mhairi C Towler
    Division of Molecular Physiology, College of Life Sciences, University of Dundee, Dundee, Scotland, UK
    Circ Res 100:328-41. 2007
    ..It is a key player in the development of new treatments for obesity, type 2 diabetes, and the metabolic syndrome...
  21. ncbi Cell cycle regulation via p53 phosphorylation by a 5'-AMP activated protein kinase activator, 5-aminoimidazole- 4-carboxamide-1-beta-D-ribofuranoside, in a human hepatocellular carcinoma cell line
    K Imamura
    Investigative Treatment Division, National Cancer Center Research Institute East, Kashiwanoha, Kashiwa, Chiba, 277-8577, Japan
    Biochem Biophys Res Commun 287:562-7. 2001
    ..Our results suggest that AICAR may regulate cell growth via p53 phosphorylation, and also indicate the possibility of p53 phosphorylation...
  22. pmc Quantitation of cellular deoxynucleoside triphosphates
    Paola Ferraro
    Department of Biology, University of Padova, 35131 Padova, Italy
    Nucleic Acids Res 38:e85. 2010
    ..We suggest that in some earlier reports ribonucleotide incorporation may have caused too high values for dGTP and dCTP...
  23. ncbi Pharmacological inhibition of AMP-activated protein kinase provides neuroprotection in stroke
    Louise D McCullough
    Department of Neurology, University of Connecticut Health Center, Farmington, Connecticut 06030, USA
    J Biol Chem 280:20493-502. 2005
    ..The ability to manipulate pharmacologically neuronal energy balance during ischemia represents an innovative approach to neuroprotection...
  24. pmc Ribonucleotides and manganese ions improve non-homologous end joining by human Polμ
    María José Martín
    Centro de Biologia Molecular Severo Ochoa CSIC UAM, 28049 Madrid, Spain
    Nucleic Acids Res 41:2428-36. 2013
    ..similarity between Polµ and terminal deoxynucleotidyl transferase (TdT) is their promiscuity in using ribonucleotides (NTPs), whose physiological significance is presently unknown...
  25. ncbi Metformin inhibits cytokine-induced nuclear factor kappaB activation via AMP-activated protein kinase activation in vascular endothelial cells
    Yoshiyuki Hattori
    Department of Endocrinology and Metabolism, Dokkyo University School of Medicine, Mibu, Tochigi 321 0293, Japan
    Hypertension 47:1183-8. 2006
    ..Thus, it might be useful to target AMPK signaling in future efforts to prevent atherogenic and inflammatory vascular disease...
  26. ncbi A possible linkage between AMP-activated protein kinase (AMPK) and mammalian target of rapamycin (mTOR) signalling pathway
    Naoki Kimura
    Biosignal Research Center, Kobe University, Kobe 657 8501, Japan
    Genes Cells 8:65-79. 2003
    ..AMP-activated protein kinase (AMPK) modulates metabolism in response to energy demand by responding to changes in AMP...
  27. ncbi Genetic impairment of AMPKalpha2 signaling does not reduce muscle glucose uptake during treadmill exercise in mice
    Stine J Maarbjerg
    Department of Exercise and Sport Sciences, Copenhagen Muscle Research Centre, University of Copenhagen, Copenhagen, Denmark
    Am J Physiol Endocrinol Metab 297:E924-34. 2009
    ..Collectively, these findings suggest that AMPKalpha2 signaling is not essential in regulating glucose uptake in mouse skeletal muscle during treadmill exercise and that other mechanisms play a central role...
  28. ncbi AMP-activated protein kinase activators can inhibit the growth of prostate cancer cells by multiple mechanisms
    Xiaoqin Xiang
    Diabetes Research Unit, Section of Endocrinology, Department of Medicine, Boston University School of Medicine, Boston, MA 02118, USA
    Biochem Biophys Res Commun 321:161-7. 2004
    ..The results suggest that AMPK is a potential target for the treatment of prostate cancer...
  29. pmc Excision of misincorporated ribonucleotides in DNA by RNase H (type 2) and FEN-1 in cell-free extracts
    Bjorn Rydberg
    Life Sciences Division, Lawrence Berkeley National Laboratory, 1 Cyclotron Road, Berkeley, CA 94720, USA
    Proc Natl Acad Sci U S A 99:16654-9. 2002
    Misincorporated ribonucleotides in DNA will cause DNA backbone distortion and may be targeted by DNA repair enzymes...
  30. pmc Mismatch repair-independent tandem repeat sequence instability resulting from ribonucleotide incorporation by DNA polymerase ε
    Alan B Clark
    Laboratory of Molecular Genetics and Laboratory of Structural Biology, National Institute of Environmental Health Sciences, NIH, DHHS, Research Triangle Park, NC 27709, United States
    DNA Repair (Amst) 10:476-82. 2011
    ..g., during the repair of nicks resulting from rNMPs in DNA. The results make interesting predictions that can be tested...
  31. ncbi Mispaired rNMPs in DNA are mutagenic and are targets of mismatch repair and RNases H
    Ying Shen
    School of Biology, Georgia Institute of Technology, Atlanta, Georgia, USA
    Nat Struct Mol Biol 19:98-104. 2012
    ..In the absence of mismatch repair and RNases H, ribonucleotide-driven gene modification increased by a factor of 47 in yeast and 77,000 in E. coli...
  32. pmc Mechanisms employed by Escherichia coli to prevent ribonucleotide incorporation into genomic DNA by Pol V
    John P McDonald
    Laboratory of Genomic Integrity, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland, United States of America
    PLoS Genet 8:e1003030. 2012
    ..Our observations suggest that errant ribonucleotides incorporated by pol V can be tolerated in the E...
  33. ncbi Long-term AICAR administration and exercise prevents diabetes in ZDF rats
    Rasmus Pold
    Medical Research Laboratory and Medical Department M Endocrinology and Diabetes, Aarhus University Hospital, Aarhus, Denmark
    Diabetes 54:928-34. 2005
    ..Our results suggest that activation of AMPK may represent a therapeutic approach to improve insulin action and prevent a decrease in beta-cell function associated with type 2 diabetes...
  34. ncbi Inhibitory effect of aminoimidazole carboxamide ribonucleotide (AICAR) on endotoxin-induced uveitis in rats
    Jun Suzuki
    Retina Service, Angiogenesis Laboratory, Department of Ophthalmology, Massachusetts Eye and Ear Infirmary, Harvard Medical School, Boston, USA
    Invest Ophthalmol Vis Sci 52:6565-71. 2011
    ..CONCLUSIONS. AICAR reduces systemic LPS susceptibility and attenuates intraocular inflammation in a rat EIU model by limiting infiltration of leukocytes, suppressing inflammatory mediators, and inhibiting the NF-κB pathway...
  35. ncbi A structural basis for metal ion mutagenicity and nucleotide selectivity in human DNA polymerase beta
    H Pelletier
    Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla 92093 0506, USA
    Biochemistry 35:12762-77. 1996
    ....
  36. pmc AMPK-independent pathways regulate skeletal muscle fatty acid oxidation
    Nicolas Dzamko
    St Vincent s Institute, 9 Princes Street, Fitzroy, Victoria 3065, Australia
    J Physiol 586:5819-31. 2008
    ..These data demonstrate that AMPK is not essential for the regulation of fatty acid oxidation by AICAR or muscle contraction...
  37. ncbi Sustained activation of AMP-activated protein kinase induces c-Jun N-terminal kinase activation and apoptosis in liver cells
    Delphine Meisse
    Hormone and Metabolic Research Unit, University of Louvain Medical School and Christian de Duve International Institute of Molecular and Cellular Pathology, Brussels, Belgium
    FEBS Lett 526:38-42. 2002
    ....
  38. ncbi Upregulation of IL-6 mRNA by IL-6 in skeletal muscle cells: role of IL-6 mRNA stabilization and Ca2+-dependent mechanisms
    Cora Weigert
    Dept of Internal Medicine, Division of Endocrinology, Metabolism, Pathobiochemistry and Clinical Chemistry, Univ of Tuebingen, Otfried Mueller Straaae 10, D 72076 Tuebingen, Germany
    Am J Physiol Cell Physiol 293:C1139-47. 2007
    ..The data suggest that IL-6 could act as autocrine factor upregulating its mRNA levels, thereby supporting its function as an exercise-activated factor in skeletal muscle cells...
  39. pmc Molecular mechanism by which AMP-activated protein kinase activation promotes glycogen accumulation in muscle
    Roger W Hunter
    MRC Protein Phosphorylation Unit, College of Life Sciences, University of Dundee, Dundee, UK
    Diabetes 60:766-74. 2011
    ..The aim of this study was to elucidate the molecular mechanism by which AMPK activation promotes muscle glycogen accumulation...
  40. pmc Contraction regulates site-specific phosphorylation of TBC1D1 in skeletal muscle
    Kanokwan Vichaiwong
    Research Division, Joslin Diabetes Center, One Joslin Place, Boston, MA 02215, U S A
    Biochem J 431:311-20. 2010
    ..AMPK and Akt regulate TBC1D1 phosphorylation, but there must be additional upstream kinases that mediate TBC1D1 phosphorylation in skeletal muscle...
  41. pmc Topoisomerase 1-mediated removal of ribonucleotides from nascent leading-strand DNA
    Jessica S Williams
    Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, NIH, DHHS, Research Triangle Park, NC 27709, USA
    Mol Cell 49:1010-5. 2013
    RNase H2-dependent ribonucleotide excision repair (RER) removes ribonucleotides incorporated during DNA replication...
  42. ncbi Activation of adenosine monophosphate activated protein kinase inhibits growth of multiple myeloma cells
    Philipp Baumann
    Department of Hematology and Oncology, Medizinische Klinik Innenstadt, Klinikum der Universitat Munchen, Germany
    Exp Cell Res 313:3592-603. 2007
    ..Our results suggest that activation of AMPK inhibits MM cell growth despite stimulation with IL-6, IGF-1, or HS-5 stromal cell conditioned medium and represents a potential new target in the therapy of MM...
  43. pmc A novel mechanism of sugar selection utilized by a human X-family DNA polymerase
    Jessica A Brown
    Department of Biochemistry, The Ohio State University, Columbus, OH 43210, USA
    J Mol Biol 395:282-90. 2010
    During DNA synthesis, most DNA polymerases and reverse transcriptases select against ribonucleotides via a steric clash between the ribose 2'-hydroxyl group and the bulky side chain of an active-site residue...
  44. ncbi Regulation of visceral and subcutaneous adipocyte lipolysis by acute AICAR-induced AMPK activation
    Nicole M Anthony
    School of Kinesiology and Health Science, York University, Toronto, Ontario, Canada
    Obesity (Silver Spring) 17:1312-7. 2009
    ..In summary, despite different fat depots eliciting distinct rates of lipolysis, acute AICAR-induced AMPK activation suppressed HSL phosphorylation/activation and exerted similar antilipolytic effects on both VC and SC adipocytes...
  45. pmc Replication of ribonucleotide-containing DNA templates by yeast replicative polymerases
    Danielle L Watt
    Laboratory of Molecular Genetics and Laboratory of Structural Biology, National Institute of Environmental Health Sciences, NIH, DHHS, Research Triangle Park, NC 27709, USA
    DNA Repair (Amst) 10:897-902. 2011
    ..The major replicative DNA polymerases of S. cerevisiae (Pols α, δ, and ɛ) incorporate substantial numbers of ribonucleotides into DNA during DNA synthesis...
  46. ncbi Role of AMPKalpha2 in basal, training-, and AICAR-induced GLUT4, hexokinase II, and mitochondrial protein expression in mouse muscle
    Sebastian B Jørgensen
    Dept of Human Physiology, Copenhagen Muscle Research Centre, Inst of Exercise and Sport Sciences, 13 Universitetsparken, Univ of Copenhagen, DK 2100 Copenhagen, Denmark
    Am J Physiol Endocrinol Metab 292:E331-9. 2007
    ..However, the alpha2-KO did not reduce training-induced increases in HKII, GLUT4, COX-1, HAD, or CS protein in WG, suggesting that AMPKalpha2 may not be essential for metabolic adaptations of skeletal muscles to exercise training...
  47. ncbi AMP-activated protein kinase alpha2 activity is not essential for contraction- and hyperosmolarity-induced glucose transport in skeletal muscle
    Nobuharu Fujii
    Research Division, Joslin Diabetes Center, Department of Medicine, Brigham and Women s Hospital and Harvard Medical School, Boston, Massachusetts 02215, USA
    J Biol Chem 280:39033-41. 2005
    ..Muscle contraction- and hyperosmolarity-induced glucose transport may be regulated by a redundant mechanism in which AMPK alpha2 is one of multiple signaling pathways...
  48. pmc Prolonged AICAR-induced AMP-kinase activation promotes energy dissipation in white adipocytes: novel mechanisms integrating HSL and ATGL
    Mandeep P Gaidhu
    School of Kinesiology and Health Science, York University, Toronto, Ontario, Canada
    J Lipid Res 50:704-15. 2009
    ..Additionally, we show novel time-dependent effects of AICAR-induced AMPK activation on lipolysis, which involves antagonistic modulation of HSL and ATGL...
  49. ncbi AMP-activated protein kinase inhibits angiotensin II-stimulated vascular smooth muscle cell proliferation
    Daisuke Nagata
    Department of Internal Medicine, Graduate School of Medicine, University of Tokyo, Tokyo, Japan
    Circulation 110:444-51. 2004
    ..Here, we investigated whether AMPK signaling has a role in the regulation of the angiotensin II (Ang II)-induced proliferation signal in rat vascular smooth muscle cells (VSMCs)...
  50. ncbi Possible CaMKK-dependent regulation of AMPK phosphorylation and glucose uptake at the onset of mild tetanic skeletal muscle contraction
    Thomas E Jensen
    Department of Human Physiology, Copenhagen Muscle Research Centre, Institute of Exercise and Sport Sciences, University of Copenhagen, Copenhagen, Denmark
    Am J Physiol Endocrinol Metab 292:E1308-17. 2007
    ....
  51. ncbi Long-term AICAR administration reduces metabolic disturbances and lowers blood pressure in rats displaying features of the insulin resistance syndrome
    Esben S Buhl
    Medical Research Laboratory, Medical Department M Endocrinology and Diabetes, Aarhus University Hospital, Aarhus Kommunehospital, DK 8000 Aarhus C, Denmark
    Diabetes 51:2199-206. 2002
    ..The present study gives additional support to the hypothesis that AMPK activation might be a potential future pharmacological strategy for treating the insulin resistance syndrome...
  52. ncbi Selective suppression of AMP-activated protein kinase in skeletal muscle: update on 'lazy mice'
    J Mu
    Department of Medicine, Howard Hughes Medical Institute, University of Pennsylvania Medical School, Clinical Research Building, 415 Curie Boulevard, Philadelphia, PA 19104 6148, USA
    Biochem Soc Trans 31:236-41. 2003
    ..We also performed microarray analysis and showed dramatic changes of transcription profile in muscles of the lazy mice. These could have a significant impact on muscle function and may contribute to the observed phenotype...
  53. ncbi Chronic treatment with 5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranoside increases insulin-stimulated glucose uptake and GLUT4 translocation in rat skeletal muscles in a fiber type-specific manner
    E S Buhl
    Medical Department M, Aarhus Kommune-hospital, Denmark
    Diabetes 50:12-7. 2001
    ..These results are comparable with the effects of chronic exercise training, and it brings the AMP-activated protein kinase into focus as a new interesting target for future pharmacological intervention in insulin-resistant conditions...
  54. ncbi AMPK activation stimulates myofibrillar protein degradation and expression of atrophy-related ubiquitin ligases by increasing FOXO transcription factors in C2C12 myotubes
    Kazuki Nakashima
    Molecular Nutrition Research Team, National Institute of Livestock and Grassland Science, Ikenoda, Tsukuba, Japan
    Biosci Biotechnol Biochem 71:1650-6. 2007
    ..These results indicate that activation of AMPK stimulates myofibrillar protein degradation through the expression of atrogin-1/MAFbx and MuRF1 by increasing FOXO transcription factors in skeletal muscles...
  55. ncbi Effects of metformin on bovine granulosa cells steroidogenesis: possible involvement of adenosine 5' monophosphate-activated protein kinase (AMPK)
    Lucie Tosca
    Unite de Physiologie de la Reproduction et des Comportements, Institut National de la Recherche Agronomique, 37380 Nouzilly, France
    Biol Reprod 76:368-78. 2007
    ..Thus, in bovine granulosa cells, metformin decreases steroidogenesis and MAPK3/MAPK1 phosphorylation through AMPK activation...
  56. ncbi C75, a fatty acid synthase inhibitor, reduces food intake via hypothalamic AMP-activated protein kinase
    Eun Kyoung Kim
    Department of Neuroscience, The Johns Hopkins University School of Medicine, 725 North Wolfe Street, Baltimore, MD 21205, USA
    J Biol Chem 279:19970-6. 2004
    ..These data indicate that modulation of FAS activity in the hypothalamus can alter energy perception via AMPK, which functions as a physiological energy sensor in the hypothalamus...
  57. ncbi Adenosine monophosphate-activated protein kinase suppresses vascular smooth muscle cell proliferation through the inhibition of cell cycle progression
    Motoyuki Igata
    Department of Metabolic Medicine, Faculty of Medical and Pharmaceutical Sciences, Kumamoto University, Kumamoto 860 8554, Japan
    Circ Res 97:837-44. 2005
    ..These data suggest for the first time that AMPK suppresses VSMC proliferation via cell cycle regulation by p53 upregulation. Therefore, AMPK activation in VSMCs may be a therapoietic target for the prevention of vascular diseases...
  58. ncbi AMPK activation increases fatty acid oxidation in skeletal muscle by activating PPARalpha and PGC-1
    Woo Je Lee
    Department of Internal Medicine, University of Ulsan College of Medicine, Seoul, Republic of Korea
    Biochem Biophys Res Commun 340:291-5. 2006
    ..These data suggest that a novel transcriptional regulatory mechanism involving PPARalpha and PGC-1 exists that is responsible for long-term stimulation of fatty acid oxidation in skeletal muscle by AICAR...
  59. ncbi Thr2446 is a novel mammalian target of rapamycin (mTOR) phosphorylation site regulated by nutrient status
    Susan W Y Cheng
    Department of Biochemistry and Molecular Biology, University College London, Gower Street, London WC1E 6BT, United Kingdom
    J Biol Chem 279:15719-22. 2004
    ..These data suggest differential phosphorylation Thr2446 and Ser2448 could act as a switch mechanism to integrate signals from nutrient status and growth factors to control the regulation of protein translation...
  60. ncbi Activation of AMP-activated protein kinase reduces hyperglycemia-induced mitochondrial reactive oxygen species production and promotes mitochondrial biogenesis in human umbilical vein endothelial cells
    Daisuke Kukidome
    Department of Metabolic Medicine, Faculty of Medical and Pharmaceutical Sciences, Kumamoto University, 1 1 1 Honjo, Kumamoto 860 8556, Japan
    Diabetes 55:120-7. 2006
    ..These results suggest that metformin normalizes hyperglycemia-induced mtROS production by induction of MnSOD and promotion of mitochondrial biogenesis through the activation of AMPK-PGC-1alpha pathway...
  61. ncbi AMP-activated protein kinase-regulated phosphorylation and acetylation of importin alpha1: involvement in the nuclear import of RNA-binding protein HuR
    Wengong Wang
    Laboratory of Cellular and Molecular Biology, NIA Intramural Research Program, National Institutes of Health, Baltimore, Maryland 21224, USA
    J Biol Chem 279:48376-88. 2004
    ..Our results point to importin alpha1 as a critical downstream target of AMPK and key mediator of AMPK-triggered HuR nuclear import...
  62. pmc Discovery of TBC1D1 as an insulin-, AICAR-, and contraction-stimulated signaling nexus in mouse skeletal muscle
    Eric B Taylor
    The Joslin Diabetes Center Section on Metabolism and Proteomics Core and Harvard Medical School, One Joslin Place, Boston, MA 02215, USA
    J Biol Chem 283:9787-96. 2008
    ..TBC1D1 is a major PAS immunoreactive protein in skeletal muscle that is phosphorylated in vivo by insulin, AICAR, and contraction. Both Akt and AMPK phosphorylate TBC1D1, but AMPK may be the more robust regulator...
  63. ncbi PPARgamma coactivator-1alpha expression during thyroid hormone- and contractile activity-induced mitochondrial adaptations
    Isabella Irrcher
    School of Kinesiology and Health Science, York University, Toronto, Ontario, Canada M3J 1P3
    Am J Physiol Cell Physiol 284:C1669-77. 2003
    ....
  64. ncbi AMPK activation stimulates autophagy and ameliorates muscular dystrophy in the mdx mouse diaphragm
    Marion Pauly
    Physiology and Experimental Medicine Heart Muscle Unit, Inserm U1046, Montpellier 1 University, Montpellier, France
    Am J Pathol 181:583-92. 2012
    ..These findings suggest that agonists of AMPK and other inducers of the autophagy-mitophagy pathway can help to promote the elimination of defective mitochondria and may thus serve as useful therapeutic agents in DMD...
  65. ncbi 2',4'-BNA/LNA aptamers: CE-SELEX using a DNA-based library of full-length 2'-O,4'-C-methylene-bridged/linked bicyclic ribonucleotides
    Yuuya Kasahara
    Graduate School of Engineering, Gunma University, 1 5 1 Tenjin cho, Kiryu, Gunma 376 8515, Japan
    Bioorg Med Chem Lett 23:1288-92. 2013
    DNA-based aptamers that contain 2'-O,4'-C-methylene-bridged/linked bicyclic ribonucleotides (B/L nucleotides) over the entire length were successfully obtained using a capillary electrophoresis systematic evolution of ligands by ..
  66. pmc Two distinct mechanisms of Topoisomerase 1-dependent mutagenesis in yeast
    Jang Eun Cho
    Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, NC 27710, USA
    DNA Repair (Amst) 12:205-11. 2013
    ..Data suggest a novel model in which rNMP-dependent hotspots are generated by sequential Top1 reactions and are consistent with rNMP-independent hotspots reflecting processing of a trapped Top1 cleavage complex...
  67. ncbi Hfq binds ribonucleotides in three different RNA-binding sites
    Victoria Murina
    Institute of Protein Research, RAS, Institutskaya 4, Pushchino 142290, Moscow Region, Russian Federation
    Acta Crystallogr D Biol Crystallogr 69:1504-13. 2013
    ..In this work, the ability of various ribonucleotides to form complexes with Hfq from Pseudomonas aeruginosa has been tested using X-ray crystallography...
  68. ncbi Effect of 5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranoside infusion on in vivo glucose and lipid metabolism in lean and obese Zucker rats
    R Bergeron
    Department of Internal Medicine, Yale University School of Medicine, New Haven, Connecticut 06510, USA
    Diabetes 50:1076-82. 2001
    ....
  69. ncbi LKB1 and the regulation of malonyl-CoA and fatty acid oxidation in muscle
    D M Thomson
    Dept of Physiology and Developmental Biology, Brigham Young Univ, Provo, UT 84602, USA
    Am J Physiol Endocrinol Metab 293:E1572-9. 2007
    ..Regardless, our results suggest that LKB1 is an important regulator of malonyl-CoA levels and fatty acid oxidation in skeletal muscle...
  70. pmc Activated ribonucleotides undergo a sugar pucker switch upon binding to a single-stranded RNA template
    Na Zhang
    Howard Hughes Medical Institute and Department of Molecular Biology and Center for Computational and Integrative Biology, Massachusetts General Hospital, 185 Cambridge Street, Boston, Massachusetts 02114, USA
    J Am Chem Soc 134:3691-4. 2012
    ..We hypothesized that ribonucleotides undergo a switch in sugar pucker upon binding to an A-type template and that this conformational switch ..
  71. pmc AMP-activated protein kinase regulates neuronal polarization by interfering with PI 3-kinase localization
    Stephen Amato
    Department of Biology, Boston University, 5 Cummington Street, Boston, MA 02215, USA
    Science 332:247-51. 2011
    ....
  72. ncbi AICAR stimulates IL-6 production via p38 MAPK in cardiac fibroblasts in adult mice: a possible role for AMPK
    Jian hai Du
    Institute of Vascular Medicine, Peking University Third Hospital, Key Laboratory of Molecular Cardiovascular Sciences, Ministry of Education, Beijing 100083, PR China
    Biochem Biophys Res Commun 337:1139-44. 2005
    ..It also dose-dependently potentiated IL-6 production in CFs, and this potentiation could be reversed by p38 MAPK inhibitor. In conclusion, AMPK activation promoted IL-6 production in CFs via p38 MAPK-dependent pathway...
  73. ncbi Leptinomimetic effects of the AMP kinase activator AICAR in leptin-resistant rats: prevention of diabetes and ectopic lipid deposition
    X Yu
    Gifford Laboratories of the Touchstone Center for Diabetes Research, University of Texas Southwestern Medical Center, Dallas, Texas, USA
    Diabetologia 47:2012-21. 2004
    ....
  74. ncbi Spatial memory formation and memory-enhancing effect of glucose involves activation of the tuberous sclerosis complex-Mammalian target of rapamycin pathway
    Pramod K Dash
    The Vivian L Smith Center for Neurologic Research and Department of Neurobiology and Anatomy, The University of Texas Medical School, Houston, Texas 77225, USA
    J Neurosci 26:8048-56. 2006
    ..Together, these findings suggest that memory formation requires the mTOR cascade and that the memory-enhancing effect of glucose involves its ability to activate this pathway...
  75. pmc Proofreading of ribonucleotides inserted into DNA by yeast DNA polymerase ɛ
    Jessica S Williams
    Laboratory of Molecular Genetics and Laboratory of Structural Biology, National Institute of Environmental Health Sciences, NIH, DHHS, Research Triangle Park, NC 27709, USA
    DNA Repair (Amst) 11:649-56. 2012
    ..3' exonuclease activity of Saccharomyces cerevisiae DNA polymerase ɛ (Pol ɛ) to proofread newly inserted ribonucleotides (rNMPs)...
  76. pmc The wild-type Schizosaccharomyces pombe mat1 imprint consists of two ribonucleotides
    Sonya Vengrova
    Marie Curie Research Institute, The Chart, Oxted, Surrey, UK
    EMBO Rep 7:59-65. 2006
    ..one stated that the imprint is a nick in the DNA, whereas our data suggested that it consists of one or two ribonucleotides incorporated into the otherwise intact DNA duplex...
  77. ncbi Activation of AMP-activated protein kinase is involved in vincristine-induced cell apoptosis in B16 melanoma cell
    Min Bin Chen
    Department of Medical Oncology, Kunshan First People s Hospital Affiliated to Jiangsu University, Kunshan, Jiangsu Province, China
    J Cell Physiol 226:1915-25. 2011
    ..We suggest that combining AMPK activator AICAR with vincristine may have potential to be used as a new therapeutic intervention against melanoma...
  78. pmc Molecular insights into DNA polymerase deterrents for ribonucleotide insertion
    Nisha A Cavanaugh
    Laboratory of Structural Biology, NIEHS, National Institutes of Health, Research Triangle Park, North Carolina 27709 2233, USA
    J Biol Chem 286:31650-60. 2011
    DNA polymerases can misinsert ribonucleotides that lead to genomic instability...
  79. pmc Ribonucleotide discrimination and reverse transcription by the human mitochondrial DNA polymerase
    Rajesh Kasiviswanathan
    Laboratory of Molecular Genetics, NIEHS, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA
    J Biol Chem 286:31490-500. 2011
    During DNA synthesis, DNA polymerases must select against ribonucleotides, present at much higher levels compared with deoxyribonucleotides...
  80. ncbi Effect of dietary ribonucleotides on infant immune status. Part 2: Immune cell development
    Rachael H Buck
    Abbott Laboratories, Ross Products Division, 3300 Stelzer Rd, Columbus, OH 43219, USA
    Pediatr Res 56:891-900. 2004
    The objective of this study was to determine whether dietary ribonucleotides alter immune cell phenotypes or function in the first year of life...
  81. ncbi Effect of dietary ribonucleotides on infant immune status. Part 1: Humoral responses
    Joseph P Schaller
    Ross Products Division, Abbott Laboratories, 3300 Stelzer Rd, Columbus, OH 43219, USA
    Pediatr Res 56:883-90. 2004
    The objective of this study was to further explore previously identified effects of supplemental ribonucleotides on infant immune status as measured by antibody responses to routine infant immunizations...
  82. pmc Role of AMP-activated protein kinase in the regulation by glucose of islet beta cell gene expression
    G da Silva Xavier
    Department of Biochemistry, School of Medical Sciences, University Walk, University of Bristol, Bristol BS8 1TD, England, United Kingdom
    Proc Natl Acad Sci U S A 97:4023-8. 2000
    ..These data suggest that glucose-induced changes in AMPK activity are necessary and sufficient for the regulation of the L-PK gene by the sugar and also play an important role in the regulation of the PPI promoter...
  83. ncbi Contraction- and hypoxia-stimulated glucose transport is mediated by a Ca2+-dependent mechanism in slow-twitch rat soleus muscle
    David C Wright
    Division of Geriatrics and Nutritional Sciences, Department of Medicine, Washington Univ School of Medicine, Applied Physiology, Campus Box 8113, 4566 Scott Ave, St Louis, MO 63110, USA
    Am J Physiol Endocrinol Metab 288:E1062-6. 2005
    ..Taken in concert, our results provide evidence that hypoxia- and contraction-stimulated glucose transport is mediated entirely through a Ca2+-dependent mechanism in rat slow-twitch muscle...
  84. pmc Deficiency of LKB1 in skeletal muscle prevents AMPK activation and glucose uptake during contraction
    Kei Sakamoto
    MRC Protein Phosphorylation Unit, School of Life Sciences, University of Dundee, Dow Street, Dundee DD1 5EH, Scotland, UK
    EMBO J 24:1810-20. 2005
    ..These studies establish the importance of LKB1 in regulating AMPK activity and cellular energy levels in response to contraction and phenformin...
  85. ncbi The effect of AMP-activated kinase activation on gonadotrophin-releasing hormone secretion in GT1-7 cells and its potential role in hypothalamic regulation of the oestrous cyclicity in rats
    S Coyral-Castel
    Unite de Physiologie de la Reproduction et des Comportements, UMR6175, INRA, Nouzilly, France
    J Neuroendocrinol 20:335-46. 2008
    ..Our findings suggest that AMPK activation may act directly at the hypothalamic level to affect fertility by modulating GnRH release and oestrous cyclicity...
  86. ncbi Effect of AICAR treatment on glycogen metabolism in skeletal muscle
    William G Aschenbach
    Research Division, Joslin Diabetes Center, Harvard Medical School, Boston, Massachusetts 02215, USA
    Diabetes 51:567-73. 2002
    ..Rather, the increased glycogen is due to the well-known effects of AICAR to increase glucose uptake...
  87. ncbi The Ca2+/calmodulin-dependent protein kinase kinases are AMP-activated protein kinase kinases
    Rebecca L Hurley
    Departments of Medicine and Biochemistry, Dartmouth Medical School and the Department of Biological Sciences, Dartmouth College, Hanover, NH 03755, USA
    J Biol Chem 280:29060-6. 2005
    ..These data indicate that the CaMKKs function in intact cells as AMPKKs, predicting wider roles for these kinases in regulating AMPK activity in vivo...
  88. pmc Dissociation of AMP-activated protein kinase and p38 mitogen-activated protein kinase signaling in skeletal muscle
    Richard C Ho
    Research Division, Joslin Diabetes Center, Boston, MA 02215, USA
    Biochem Biophys Res Commun 362:354-9. 2007
    ..Furthermore, AMPKalpha2i TG mice lacking contraction-stimulated AMPK activity have normal p38 MAPK phosphorylation. These results suggest that p38 MAPK is not a downstream component of AMPK-mediated signaling in skeletal muscle...
  89. ncbi Coordinated maintenance of muscle cell size control by AMP-activated protein kinase
    Louise Lantier
    Institut Cochin, Universite Paris Descartes, CNRS UMR 8104, Paris, France
    FASEB J 24:3555-61. 2010
    ..Our results uncover the role of AMPK in the maintenance of muscle cell size control and highlight the crosstalk between AMPK and mTOR/p70S6K signaling pathways coordinating a metabolic checkpoint on cell growth...
  90. ncbi Abnormal metabolism flexibility in response to high palmitate concentrations in myotubes derived from obese type 2 diabetic patients
    Magali Kitzmann
    INSERM, ESPRI25 Muscle et pathologies, Montpellier, F 34295, France
    Biochim Biophys Acta 1812:423-30. 2011
    ..Interestingly, metformin treatment and mitochondrial inhibition by antimycin induced increased lipid content in control myotubes. We conclude that T2D myotubes display an impaired capacity to respond to metabolic stimuli...
  91. pmc An error-prone family Y DNA polymerase (DinB homolog from Sulfolobus solfataricus) uses a 'steric gate' residue for discrimination against ribonucleotides
    Angela M DeLucia
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520 8114, USA
    Nucleic Acids Res 31:4129-37. 2003
    ..of the A and B families, and reverse transcriptases, share a common mechanism for preventing incorporation of ribonucleotides: a highly conserved active site residue obstructing the position that would be occupied by a 2' hydroxyl ..
  92. pmc Upregulation of mitochondrial uncoupling protein-2 by the AMP-activated protein kinase in endothelial cells attenuates oxidative stress in diabetes
    Zhonglin Xie
    Division of Endocrinology and Diabetes, Department of Medicine, University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA
    Diabetes 57:3222-30. 2008
    ..The present study was conducted to determine how AMPK activation suppressed tyrosine nitration of prostacyclin synthase in diabetes...
  93. ncbi Eicosapentaenoic acid stimulates AMP-activated protein kinase and increases visfatin secretion in cultured murine adipocytes
    Silvia Lorente-Cebrián
    Department of Nutrition, Food Science, Physiology and Toxicology, University of Navarra, Pamplona, Spain
    Clin Sci (Lond) 117:243-9. 2009
    ..The results of the present study suggest that the stimulatory action of EPA on visfatin production involves AMPK activation in adipocytes...
  94. pmc AMPKalpha2 deletion causes aberrant expression and activation of NAD(P)H oxidase and consequent endothelial dysfunction in vivo: role of 26S proteasomes
    Shuangxi Wang
    Section of Endocrinology and Diabetes, Department of Medicine, University of Oklahoma Health Science Center, Oklahoma City, OK 73104, USA
    Circ Res 106:1117-28. 2010
    ..Recent studies suggest that AMPK activation improves endothelial function by counteracting oxidative stress in endothelial cells. How AMPK suppresses oxidative stress remains to be established...
  95. ncbi AMP-activated protein kinase is highly expressed in neurons in the developing rat brain and promotes neuronal survival following glucose deprivation
    C Culmsee
    Laboratory of Neurosciences, National Institute on Aging, Baltimore, MD 21224, USA
    J Mol Neurosci 17:45-58. 2001
    ..These findings suggest that AMPK can protect neurons against metabolic and excitotoxic insults relevant to the pathogenesis of several different neurodegenerative conditions...
  96. ncbi Metformin prevents progression of heart failure in dogs: role of AMP-activated protein kinase
    Hideyuki Sasaki
    Department of Cardiovascular Medicine, National Cardiovascular Center, Suita, Osaka, Japan
    Circulation 119:2568-77. 2009
    ..Because AMPK also is activated in animal models of heart failure, we investigated whether metformin decreases cardiomyocyte apoptosis and attenuates the progression of heart failure in dogs...
  97. pmc AICAR activates AMPK and alters PIP2 association with the epithelial sodium channel ENaC to inhibit Na+ transport in H441 lung epithelial cells
    Oliver J Mace
    Division of Basic Medical Sciences, St George s University of London, Cranmer Terrace, London SW17 0RE, UK
    J Physiol 586:4541-57. 2008
    ..Thus, when PIP(2)-ENaC subunit interactions were compromised, ENaC protein retrieval was initiated, indicating that AMPK can modulate ENaC P(o) and N...
  98. ncbi Effect of acute activation of 5'-AMP-activated protein kinase on glycogen regulation in isolated rat skeletal muscle
    Licht Miyamoto
    Department of Medicine and Clinical Science, Kyoto University, Sakyo ku, Kyoto 606 8501, Japan
    J Appl Physiol 102:1007-13. 2007
    ....
  99. ncbi Effects of alpha-AMPK knockout on exercise-induced gene activation in mouse skeletal muscle
    Sebastian B Jørgensen
    Copenhagen Muscle Research Centre, Department of Human Physiology, Institute of Exercise and Sport Sciences, University of Copenhagen, Copenhagen, Denmark
    FASEB J 19:1146-8. 2005
    ....
  100. ncbi Resolution enhanced homonuclear carbon decoupled triple resonance experiments for unambiguous RNA structural characterization
    Kwaku T Dayie
    Department of Molecular Genetics and Program in Structural Biology, Lerner Research Institute, Cleveland Clinic Foundation, Cleveland, OH 44195, USA
    J Biomol NMR 32:129-39. 2005
    ....
  101. ncbi Knockout of the alpha2 but not alpha1 5'-AMP-activated protein kinase isoform abolishes 5-aminoimidazole-4-carboxamide-1-beta-4-ribofuranosidebut not contraction-induced glucose uptake in skeletal muscle
    Sebastian B Jørgensen
    Copenhagen Muscle Research Centre, Department of Human Physiology, 13 Universitetsparken, University of Copenhagen, DK 2100 Copenhagen, Denmark
    J Biol Chem 279:1070-9. 2004
    ..Alternatively, neither alpha-isoform of AMPK is involved in contraction-induced muscle glucose uptake...

Research Grants62

  1. Human Umami Taste: Genetics of Psychophysical Traits
    Paul A S Breslin; Fiscal Year: 2012
    ..found within taste receptor cells have been associated with cell signaling in response to amino acids and ribonucleotides: (i) TAS1Rs, (ii) metabotropic-GluRs, and (iii) ionotropic-NMDA receptors...
  2. Global analysis of mRNA decay in Bacillus subtilis
    David H Bechhofer; Fiscal Year: 2013
    ..are synthesized, needs to be turned over rapidly to adapt to changing environments and to recycle the pool of ribonucleotides needed for new RNA synthesis...
  3. Role of miR-30d in insulin gene expression in pancreatic beta cells
    Xiaoqing Tang; Fiscal Year: 2010
    ..MiRNAs are small noncoding ribonucleotides that bind mRNAs and function mainly as translational repressors in mammals...
  4. Electronic Structure and Reactions of Fe-oxo Enzymes
    Louis Noodleman; Fiscal Year: 2010
    ..RNRs catalyze the transformation of ribonucleotides to deoxyribonucleotides, the first required and often rate limiting step in DNA synthesis...
  5. Topoisomerase 1 and mutagenesis in yeast
    Sue Jinks-Robertson; Fiscal Year: 2013
    ..A similar, short-deletion signature has been associated with the failure to remove ribonucleotides (rNMPs) misincorporated into DNA, and this signature likewise depends on Top1 activity...
  6. Primer Synthesis and Handoff by Bacteriophage T7 DNA Primase
    ALFREDO JOSE HERNANDEZ; Fiscal Year: 2013
    ..This essential class of enzymes catalyzes the synthesis of ribonucleotides that serve as primers for DNA polymerase for the initiation of Okazaki fragment synthesis...
  7. BIOLOGICAL METAL CLUSTERS--BIOPHYSICAL & MODEL STUDIES
    Michael Maroney; Fiscal Year: 1991
    ..The proteins serve diverse functions: oxygen transport, regulation of phosphate metabolism, conversion of ribonucleotides to deoxyribonucleotides ( a key step in DNA synthesis), and oxidation of methane to methanol...
  8. DEOXYRIBONUCLEOTIDE METABOLISM IN ESCHERICHIA COLI
    James Fuchs; Fiscal Year: 1992
    Ribonucleotide reductases catalyze the reduction of ribonucleotides to deoxyribonucleotides...
  9. Investigating the structural assembly of RNR multimers
    Chris G Dealwis; Fiscal Year: 2013
    ..reductase (RR) key to maintaining life as it catalyzes the slowest step in de novo DNTP synthesis by reducing ribonucleotides to deoxy ribonucleotides...
  10. Structural Biology of the Catalytic Domain of Bacteriophage T4 Primase
    David Rovnyak; Fiscal Year: 2009
    ..The binding of the C-terminal domain to ribonucleotides and oligoribonucleotides will be investigated both by chemical shift perturbations of the backbone atoms, and ..
  11. Ribonucleotide Reductase Inhibitors for Restenosis
    Howard Elford; Fiscal Year: 2009
    ..As such, we propose that the conversion of ribonucleotides to deoxyribonucleotides is an attractive therapeutic target to control cell proliferation and will reduce ..
  12. METABOLIC DETERMINANTS OF HEMATOPOIETIC CELL DEVELOPMENT
    Daniel Wright; Fiscal Year: 1999
    ..changes caused by nutrient deprivation, and studies of these organisms have shown that depletion of guanine ribonucleotides (e.g. GTP) is a critical metabolic event int he onset of development...
  13. REPLICATION OF CHLOROPLAST DNA
    KRISHNA TEWARI; Fiscal Year: 1992
    ..will be analyzed for precisely mapping the initiation sites of replication and for the presence or absence of ribonucleotides as primers...
  14. ACTIVATION OF CHEMOTHERAPEUTIC AGENTS IN LEUKEMIC CELLS
    Donna Shewach; Fiscal Year: 1992
    ..Since deoxycytidine kinase is allosterically regulated, the effects of deoxy- and ribonucleotides on the purified enzyme activity will be studied using the normal cellular concentrations of these nucleotides...
  15. Multi-site Trial of Azathioprine Dosing in Crohn Disease
    Stephen Hanauer; Fiscal Year: 2003
    ..enzymatic pathway, thiopurine methyltransferase (TPMT) catalyzes formation of 6-methyl-mercaptopurine ribonucleotides (6-MMPR), metabolites that are therapeutically inactive and potentially hepatotoxic...
  16. Multi-site Trial of Azathioprine Dosing in Crohn Disease
    Stephen Hanauer; Fiscal Year: 2004
    ..enzymatic pathway, thiopurine methyltransferase (TPMT) catalyzes formation of 6-methyl-mercaptopurine ribonucleotides (6-MMPR), metabolites that are therapeutically inactive and potentially hepatotoxic...
  17. Iron Trafficking to Ribonucleotide Reductases
    DANIEL KOSMAN; Fiscal Year: 2006
    ..While not directly involved in the reduction of ribonucleotides to deoxyribonucleotides, this prosthetic group is essential to enzyme activity...
  18. POTENTIAL NEW TOPICAL ANTI-PSORIASIS AGENTS
    Howard Elford; Fiscal Year: 1991
    ..The reduction of ribonucleotides to deoxyribonucleotides, catalyzed by ribonucleotide reductase, is a crucial and rate limiting step in the ..
  19. HIGH RESOLUTION STRUCTURE OF MULTI COMPONENT VIRUSES
    John Johnson; Fiscal Year: 1993
    ..A total of 11 contiguous ribonucleotides were modeled in the icosahedral asymmetric unit of 3482 nucleotides. This structure will be refined at 2...
  20. PURINE METABOLISM AND CHEMOTHERAPY IN GIARDIA LAMBLIA
    RANDOLPH BERENS; Fiscal Year: 1990
    ..These include the lack of interconversion between ribonucleotides, the transport of nucleoside analogues, and the requirement for deoxyribonucleosides...
  21. IRON SITE OF RIBONUCLEOTIDE REDUCTASE
    Grant Bunker; Fiscal Year: 1992
    ..enzyme ribonucleotide reductase (RR) catalyzes an essential step in the biosynthesis of DNA: the reduction of ribonucleotides to deoxyribonucleotides. Ribonucleotide reductase activity is essential for the survival of all cells...
  22. Is there a Class IV ribonucleotide reductase?
    Pamela Riggs Gelasco; Fiscal Year: 2003
    ..This so called Class I cofactor then participates in the conversion of ribonucleotides to deoxyribonucleotides, the rate limiting step in DNA synthesis...
  23. RIBONUCLEOTIDE REDUCTASE OF THE MALARIA PARASITE
    Debopam Chakrabarti; Fiscal Year: 2000
    ..step in the de novo synthesis of deoxyribonucleotides by the direct reduction of the corresponding ribonucleotides. The important role of RNR in DNA synthesis and cell division makes this enzyme an excellent target for ..
  24. INHIBITORS OF HUMAN RIBONUCLEOTIDE REDUCTASE
    RAYMOND BLAKLEY; Fiscal Year: 1980
    ..especially analogs of dATP, will be synthesized by large-scale enzymic reduction of the corresponding ribonucleotides. The latter will be obtained by chemical phosphorylation of available nucleosides, especially ribonucleotide ..
  25. Crosslinking Ribonucleotide Reductase Subunits to Study PCET Within
    Richard Kelley; Fiscal Year: 2009
    Ribonucleotide reductases (RNRs) are responsible for reducing ribonucleotides (NDPs) to deoxyribonucleotides (dNDPs) and are required in all organisms in order to make the monomeric precursors used in DNA replication and repair...
  26. INHIBITION OF RIBONUCLEOTIDE REDUCTASE IN GLIOMAS
    Wagner dos Santos; Fiscal Year: 2005
    ..Ribonucleotide reductase (RNR) is a key enzyme for converting ribonucleotides into deoxyribonucleotides, an activity crucial for DNA replication...
  27. RIBONUCLEOTIDE REDUCTASE AND CANCER CHEMOTHERAPY
    Howard Elford; Fiscal Year: 1980
    The conversion of ribonucleotides to deoxyribonucleotides is a critical step in the pathway leading to the formation of DNA. Our primary objective is the rational development of chemotherapeutic agents directed against this reaction...
  28. MECHANISM AND INHIBITION OF RIBONUCLEOTIDE REDUCTASE
    JoAnne Stubbe; Fiscal Year: 1980
    This research proposal is concerned with a detailed study of the mechanism of reduction of ribonucleotides by ribonucleotide reductase and the design of potential suicide inhibitors for this enzyme...
  29. NUCLEOTIDE METABOLISM & PROMOTION OF CARCINOGENESIS
    Michael Lea; Fiscal Year: 1990
    ..Increased synthesis of pyrimidine ribonucleotides and decreased synthesis of purine ribonucleotides has been observed in rat liver on a diet high in orotate or ..
  30. REGULATION OF DORMANCY IN M TUBERCULOSIS
    Harvey Rubin; Fiscal Year: 2003
    ..specific animal or culture model of dormancy: These are: 1) the ribonucleotide reductase system that reduces ribonucleotides to deoxyribonucleotides--the rate limiting step in DNA synthesis, 2) the cytochrome bd oxidase system ..
  31. PLASMA PURINE RATIO--A NEW BIOMARKER FOR CANCER RISK
    James Sherley; Fiscal Year: 2002
    ..Recently, p53 has been shown to regulate the production of cellular guanine ribonucleotides (rGNPs). RGNPs function in growth factor signal transduction...
  32. Isotopically Labeled Nucleotides for Biomolecular NMR
    James Williamson; Fiscal Year: 2007
    ..The technology involves the efficient single-pot synthesis of labeled ribonucleotides from labeled precursors using a series of enzymatic transformations...
  33. Directed Evolution of DNA and RNA Polymerases
    REBECCA HOLMBERG; Fiscal Year: 2005
    ..c). Finally, a selection with the mutant library will be executed for incorporation of two types of unnatural ribonucleotides (Specific Aim II...
  34. REDUCTION OF RIBONUCLEOTIDES IN NORMAL AND TUMOR TISSUES
    JAY ROTH; Fiscal Year: 1980
    ..Assays for CDP and UDP reductase activity are being carried out on these cells and the enzyme is being isolated and purified from this source...
  35. Physiology of Malonyl-CoA in Muscle during Exercise
    WILLIAM WINDER; Fiscal Year: 2006
    ..The results will provide additional rationale for the use of regular exercise in preventing and treating type 2 diabetes and obesity. ..
  36. Regulation of Mammalian Oocyte Maturation
    Stephen Downs; Fiscal Year: 2007
    ..Furthermore, these studies will benefit the development of in vitro systems where either meiotic arrest or completion of meiotic maturation is the desired endpoint. ..
  37. Regulation of Skeletal Muscle LKB1
    WILLIAM WINDER; Fiscal Year: 2010
    ..The proposed studies will provide additional rationale for convincing a predominantly sedentary population of the importance of regular exercise in combating inactivity-related diseases. ..
  38. Synthesis and testing of DNA bending agents
    Steven Firestine; Fiscal Year: 2004
    ..abstract_text> ..
  39. Targeting the oligomerization of human ABCG2
    Jian Ting Zhang; Fiscal Year: 2009
    ..This work may also lead us to the discovery of a new class of therapeutic agents that can help overcome drug-resistant cancers. ..
  40. Venular leukocyte adhesion, impaired arteriolar vasoreactivity, and intestinal IR
    Ronald J Korthuis; Fiscal Year: 2010
    ..Given the importance of the endothelium in regulating vascular tone, these fundamentally important findings have enormous implications for our understanding of blood flow dysregulation in conditions characterized by I/R. ..
  41. Energy dysregulation: The role of AMPK in stroke
    LOUISE DEBORAH MCCULLOUGH; Fiscal Year: 2010
    ..A multi-faceted approach is clearly needed to develop efficacious neuroprotective agents that will benefit stroke patients. Manipulation of AMPK levels represents a novel approach to neuroprotection. ..
  42. Enhancement of methotrexate uptake in childhood ALL
    John McGuire; Fiscal Year: 2009
    ..If successful, this research could provide a way to increase the effectiveness of methotrexate, a key drug used in treating childhood acute lymphoblastic leukemia, and thus increase the cure rate in this disease. ..
  43. Working memory deficits following brain trauma
    Pramod Dash; Fiscal Year: 2009
    ..An understanding of the mechanisms by which TBI alters dopamine signaling is critical for the development of mechanism-based intelligent pharmacological treatments for working memory deficits for brain trauma patients. ..
  44. SPECTROSCOPIC STUDIES OF LIGHT-DRIVEN ELECTRON TRANSFER
    BRIDGETTE ANNE BARRY; Fiscal Year: 2010
    ..RNR catalyzes the reduction of ribonucleotides to deoxynucleotides. In E. coli RNR, a redox-active tyrosine, Y122, is proposed to be a radical initiator...
  45. The function of RhoBTB proteins
    STEPHEN PAUL SOLTOFF; Fiscal Year: 2010
    ..The results of these studies may reveal a new pathway involved in the development of cancer and advance our understanding of the basic biology of transformation. ..
  46. Mitochrondrial Dysfunction: Role in Metabolic Syndrome
    Gerald Shulman; Fiscal Year: 2008
    ..The program is coordinated by frequent meetings of the program faculty and research workers bdnging together these diverse approaches to address a common goal. ..
  47. MECHANISM OF DRUG BINDING TO P-GLYCOPROTEIN
    Jian Ting Zhang; Fiscal Year: 2001
    ..This work may lead us to the discovery of a new family of chaperone proteins associated with ribosomes that are important in determining the folding of membrane proteins co-translationally. ..
  48. Leukemia inhibitor factor in skeletal muscle regrowth
    Espen Spangenburg; Fiscal Year: 2003
    ..Identification of a cytokine and a signaling mechanism that may regulate muscle growth could lead to other counter-measures to ultimately prevent muscle wasting. ..
  49. PRECONDITIONING: PMN ADHESION AND MICROVASCULAR INJURY
    RONALD KORTHUIS; Fiscal Year: 2003
    ....
  50. Identification of a new protein that binds Src and PKC
    STEPHEN SOLTOFF; Fiscal Year: 2004
    ..Future efforts will determine if this new protein alters cell morphology, tight junction formation, or other functions in salivary cells. ..
  51. RNAi Technology to Study In Vivo Muscle Metabolism
    LAURIE GOODYEAR; Fiscal Year: 2005
    ..Ultimately, this approach could provide the basis for treatment of skeletal muscle insulin resistance and other chronic muscle diseases such as the dystrophies. ..
  52. THERMODYNAMICS OF BIOCHEMICAL SYSTEMS
    ROBERT ALBERTY; Fiscal Year: 2005
    ..This quantitative treatment of the literature data on biochemical reactions will uncover errors because deltafG'0 values can be determined by use of two or more biochemical reactions, which should give the same answer. ..
  53. RESEARCH TRAINING IN PHYSIOLOGICAL ADAPTATION TO STRESS
    Leonard Jefferson; Fiscal Year: 2005
    ..abstract_text> ..
  54. FACILITY CONSTRUCTION EXPANSION- MONNELL CHEMICAL SENSES
    Gary Beauchamp; Fiscal Year: 2003
    ....
  55. ARK5 AMP-activated protein kinase and glucose transport
    Jonathan Fisher; Fiscal Year: 2007
    ..The proposed training will develop the candidate in his transition to an independent researcher and is critical to the candidate's long-term ability to contribute to diabetes research ..
  56. AMPK: Impact on Metabolism and Disease
    LAURIE GOODYEAR; Fiscal Year: 2006
    ..unreadable] [unreadable] [unreadable]..
  57. Polymer-Nucleotide Complexes with Cytotoxic Activity
    Serguei Vinogradov; Fiscal Year: 2007
    ..Application of the drug forms may help to prevent many of the known chemotherapy side effects. Data accumulated in these studies can be directly used for design of better systemic formulations of cytotoxic nucleotide drugs. ..
  58. The Natural History of Pediatric Crohn's Disease
    Marla Dubinsky; Fiscal Year: 2008
    ..abstract_text> ..
  59. GENETICS & BIOCHEMISTRY OF NEURONAL PAS DOMAIN PROTEINS
    STEVEN MCKNIGHT; Fiscal Year: 2008
    ..abstract_text> ..
  60. Bioengineering of the blood-brain barrier permeability
    Serguei Vinogradov; Fiscal Year: 2008
    ..unreadable] [unreadable]..
  61. Parental Expression of Immune Responses In Pediatric Crohn's Disease
    Marla Dubinsky; Fiscal Year: 2008
    ..unreadable] [unreadable]..
  62. MOLECULAR BASES OF MULTIDRUG RESISTANCE IN CANCERS
    Jian Ting Zhang; Fiscal Year: 2001
    ..Additionally, this study will increase our understanding of how specific protein topological structures are related to transport functions in general. ..