uridine triphosphate

Summary

Summary: Uridine 5'-(tetrahydrogen triphosphate). A uracil nucleotide containing three phosphate groups esterified to the sugar moiety.

Top Publications

  1. ncbi Mechanisms of release of nucleotides and integration of their action as P2X- and P2Y-receptor activating molecules
    Eduardo R Lazarowski
    Department of Pharmacology, University of North Carolina School of Medicine, CB 7365, Chapel Hill, NC 27599, USA
    Mol Pharmacol 64:785-95. 2003
  2. pmc RNA polymerase III detects cytosolic DNA and induces type I interferons through the RIG-I pathway
    Yu Hsin Chiu
    Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390 9148, USA
    Cell 138:576-91. 2009
  3. ncbi Nucleotide-induced Ca2+ signaling in sustentacular supporting cells of the olfactory epithelium
    Thomas Hassenklöver
    Department of Neurophysiology and Cellular Biophysics, University of Gottingen, Humboldtallee 23, Gottingen, Germany
    Glia 56:1614-24. 2008
  4. pmc Uridine triphosphate (UTP) induces profibrotic responses in cardiac fibroblasts by activation of P2Y2 receptors
    Oscar O Braun
    Department of Pharmacology, University of California San Diego, La Jolla, California 92093, USA
    J Mol Cell Cardiol 49:362-9. 2010
  5. ncbi P2Y2 and P2Y4 receptors regulate pancreatic Ca(2+)-activated K+ channels differently
    Susanne E Hede
    Institute of Molecular Biology and Physiology, The August Krogh Building, University of Copenhagen, Universitetsparken 13, 2100, Copenhagen Ø, Denmark
    Pflugers Arch 450:429-36. 2005
  6. pmc Activation of purinergic receptors induces proliferation and neuronal differentiation in Swiss Webster mouse olfactory epithelium
    C Jia
    Department of Pharmacology and Toxicology, Michigan State University, East Lansing, MI 48823, USA
    Neuroscience 163:120-8. 2009
  7. ncbi Calcium-activated K(+) channel (K(Ca)3.1) activity during Ca(2+) store depletion and store-operated Ca(2+) entry in human macrophages
    Ya Dong Gao
    Institut fur Physiologie und Pathophysiologie, Universitat Marburg, Deutschhausstr 2, 35037 Marburg, Germany
    Cell Calcium 48:19-27. 2010
  8. pmc Regulation of epidermal homeostasis through P2Y2 receptors
    C J Dixon
    Department of Human Anatomy and Cell Biology, University of Liverpool, New Medical School, Ashton Street, Liverpool L69 3GE
    Br J Pharmacol 127:1680-6. 1999
  9. ncbi Differential regulation of airway mucin gene expression and mucin secretion by extracellular nucleotide triphosphates
    Y Chen
    Center for Comparative Respiratory Biology and Medicine, University of California at Davis, One Shields Avenue, Davis, CA 95616, USA
    Am J Respir Cell Mol Biol 25:409-17. 2001
  10. pmc Functional distribution of Ca2+-coupled P2 purinergic receptors among adrenergic and noradrenergic bovine adrenal chromaffin cells
    Angelo R Tomé
    Center for Neurosciences and Cell Biology, University of Coimbra, Coimbra, Portugal
    BMC Neurosci 8:39. 2007

Research Grants

Detail Information

Publications210 found, 100 shown here

  1. ncbi Mechanisms of release of nucleotides and integration of their action as P2X- and P2Y-receptor activating molecules
    Eduardo R Lazarowski
    Department of Pharmacology, University of North Carolina School of Medicine, CB 7365, Chapel Hill, NC 27599, USA
    Mol Pharmacol 64:785-95. 2003
  2. pmc RNA polymerase III detects cytosolic DNA and induces type I interferons through the RIG-I pathway
    Yu Hsin Chiu
    Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390 9148, USA
    Cell 138:576-91. 2009
    ..These results suggest that RNA Pol-III is a cytosolic DNA sensor involved in innate immune responses...
  3. ncbi Nucleotide-induced Ca2+ signaling in sustentacular supporting cells of the olfactory epithelium
    Thomas Hassenklöver
    Department of Neurophysiology and Cellular Biophysics, University of Gottingen, Humboldtallee 23, Gottingen, Germany
    Glia 56:1614-24. 2008
    ..Our findings suggest that extracellular nucleotides in the OE activate SCs via P2Y2/P2Y4-like receptors and initiate a characteristic intraepithelial Ca2+ wave...
  4. pmc Uridine triphosphate (UTP) induces profibrotic responses in cardiac fibroblasts by activation of P2Y2 receptors
    Oscar O Braun
    Department of Pharmacology, University of California San Diego, La Jolla, California 92093, USA
    J Mol Cell Cardiol 49:362-9. 2010
    ..We conclude that P2Y2 receptor activation is profibrotic in CFs; thus inhibition of P2Y2 receptors may provide a novel means to diminish fibrotic remodeling and turnover of extracellular matrix in the heart...
  5. ncbi P2Y2 and P2Y4 receptors regulate pancreatic Ca(2+)-activated K+ channels differently
    Susanne E Hede
    Institute of Molecular Biology and Physiology, The August Krogh Building, University of Copenhagen, Universitetsparken 13, 2100, Copenhagen Ø, Denmark
    Pflugers Arch 450:429-36. 2005
    ..Taken together, this study shows that there are different interactions between the subtypes of P2Y purinergic receptors and different Ca(2+)-activated K+ channels...
  6. pmc Activation of purinergic receptors induces proliferation and neuronal differentiation in Swiss Webster mouse olfactory epithelium
    C Jia
    Department of Pharmacology and Toxicology, Michigan State University, East Lansing, MI 48823, USA
    Neuroscience 163:120-8. 2009
    ..We propose that extracellular ATP released upon injury could induce proliferation and promote the neuroregeneration of the olfactory epithelium...
  7. ncbi Calcium-activated K(+) channel (K(Ca)3.1) activity during Ca(2+) store depletion and store-operated Ca(2+) entry in human macrophages
    Ya Dong Gao
    Institut fur Physiologie und Pathophysiologie, Universitat Marburg, Deutschhausstr 2, 35037 Marburg, Germany
    Cell Calcium 48:19-27. 2010
    ..1 channel current and hyperpolarization, providing the necessary electrochemical driving force for prolonged Ca(2+) signaling and store repletion...
  8. pmc Regulation of epidermal homeostasis through P2Y2 receptors
    C J Dixon
    Department of Human Anatomy and Cell Biology, University of Liverpool, New Medical School, Ashton Street, Liverpool L69 3GE
    Br J Pharmacol 127:1680-6. 1999
    ..7. These findings indicate that P2Y2 receptors play a major role in epidermal homeostasis, and may provide novel targets for therapy of proliferative disorders of the epidermis, including psoriasis...
  9. ncbi Differential regulation of airway mucin gene expression and mucin secretion by extracellular nucleotide triphosphates
    Y Chen
    Center for Comparative Respiratory Biology and Medicine, University of California at Davis, One Shields Avenue, Davis, CA 95616, USA
    Am J Respir Cell Mol Biol 25:409-17. 2001
    ..These findings are the first demonstration that UTP, a pyrimidine nucleotide triphosphate, can enhance both mucin secretion and mucin gene expression through different signaling pathways...
  10. pmc Functional distribution of Ca2+-coupled P2 purinergic receptors among adrenergic and noradrenergic bovine adrenal chromaffin cells
    Angelo R Tomé
    Center for Neurosciences and Cell Biology, University of Coimbra, Coimbra, Portugal
    BMC Neurosci 8:39. 2007
    ..We have taken advantage of the actions of the purinergic agonists ATP and UTP on cytosolic free Ca2+ concentration ([Ca2+]i) to determine whether P2X and P2Y receptors might be asymmetrically distributed among AD and NA chromaffin cells...
  11. pmc Agonist-induced calcium entry correlates with STIM1 translocation
    Kehinde Ross
    Dermatological Sciences, Institute of Cellular Medicine, Medical School, Newcastle University, Newcastle upon Tyne, United Kingdom
    J Cell Physiol 211:569-76. 2007
    ..Thus by differentially regulating store-refilling after agonist-mediated depletion, LPA and UTP may exert distinct effects on the duration of STIM1 localization at the plasma membrane, and thus, on the magnitude and duration of ACE...
  12. ncbi ATP stimulates interleukin-6 production via P2Y receptors in human HaCaT keratinocytes
    Hirohide Yoshida
    Department of Cellular Signaling, Tohoku University, Aoba 6 3, Aramaki, Aoba ku, Sendai 980 8578, Japan
    Eur J Pharmacol 540:1-9. 2006
    ..These results suggest that 1) ATP is released from HaCaT cells upon physical stimulation and may act as an autocrine molecule, and 2) the stimulation of P2Y receptors causes IL-6 production via mRNA expression through [Ca2+]i elevation...
  13. ncbi Two distinct ATP receptors activate calcium entry and internal calcium release in bovine chromaffin cells
    F Reichsman
    Program in Molecular and Cellular Biology, University of Massachusetts, Amherst 01003, USA
    J Neurochem 65:2080-6. 1995
    ..The low response of both receptors to ADP distinguishes them from the ATP receptor on these cells that inhibits voltage-dependent Ca2+ current and secretion...
  14. ncbi Inhibition of amiloride-sensitive epithelial Na(+) absorption by extracellular nucleotides in human normal and cystic fibrosis airways
    M Mall
    Universitäts Kinderklinik, Albert Ludwigs Universitat Freiburg, Freiburg, Germany
    Am J Respir Cell Mol Biol 23:755-61. 2000
    ..Because ATP and UTP inhibit Na(+) absorption and stimulate Cl(-) secretion simultaneously, extracellular nucleotides could have a dual therapeutic effect, counteracting the ion transport defect in CF lung disease...
  15. ncbi Identification of the P2Y(12) receptor in nucleotide inhibition of exocytosis from bovine chromaffin cells
    Steven J Ennion
    Department of Biomedical Sciences, University of Sheffield, Alfred Denny Building, Western Bank, Sheffield, S10 2TN, United Kingdom
    Mol Pharmacol 66:601-11. 2004
    ..These results identify the P2Y(12) purine receptor as a key component of the nucleotide inhibitory pathway and also demonstrate the involvement of a UTP-sensitive G(i/o) -coupled pyrimidine receptor...
  16. ncbi Regulation of mucin secretion from in vitro cellular models
    C William Davis
    Department of Cell and Molecular Physiology, and Cystic Fibrosis Pulmonary Research and Treatment Center, 6009 Thurston Bowles, University of North Carolina, Chapel Hill, NC 27599 7248, USA
    Novartis Found Symp 248:113-25; discussion 125-31, 277-82. 2002
    ....
  17. ncbi Cellular injury induces activation of MAPK via P2Y receptors
    LingLing Yang
    Departments of Biochemistry and Ophthalmology, Boston University School of Medicine, Boston University, Boston, Massachusetts 02118, USA
    J Cell Biochem 91:938-50. 2004
    ..Adenosine triphosphate (ATP), uridine triphosphate (UTP), adenosine diphosphate (ADP), and uridine diphosphate (UDP) promoted activation by 2 min with similar ..
  18. pmc Polarized signaling via purinoceptors in normal and cystic fibrosis airway epithelia
    A M Paradiso
    Cystic Fibrosis Pulmonary Research and Treatment Center, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    J Gen Physiol 117:53-67. 2001
    ....
  19. pmc Purinergic receptor activation evokes neurotrophic factor neuropeptide Y release from neonatal mouse olfactory epithelial slices
    Shami Kanekar
    Department of Physiology, University of Utah, Salt Lake City, Utah, USA
    J Neurosci Res 87:1424-34. 2009
    ....
  20. pmc Modulation of endothelial cell migration by extracellular nucleotides: involvement of focal adhesion kinase and phosphatidylinositol 3-kinase-mediated pathways
    Elzbieta Kaczmarek
    Beth Israel Deaconess Medical Center, Department of Medicine, Harvard Medical School, Boston, MA 02215, USA
    Thromb Haemost 93:735-42. 2005
    ..We propose that P2Y2 and possibly P2Y4 receptors mediate those responses that are important in vascular inflammation, atherosclerosis and angiogenesis...
  21. pmc Structure of the mitochondrial editosome-like complex associated TUTase 1 reveals divergent mechanisms of UTP selection and domain organization
    Jason Stagno
    Department of Molecular Biology and Biochemistry, University of California, Irvine, CA 92697, USA
    J Mol Biol 399:464-75. 2010
    ..Because MEAT1 is essential for the viability of the bloodstream and insect forms of Trypanosoma brucei, the unique organization of its active site renders this protein an attractive target for trypanocide development...
  22. ncbi Purinergic P2Y6 receptors induce Ca2+ and CFTR dependent Cl- secretion in mouse trachea
    Rainer Schreiber
    Institut fur Physiologie, Universitat Regensburg, Germany
    Cell Physiol Biochem 16:99-108. 2005
    ..In summary, activation of luminal P2Y6 receptors in the airways shifts electrolyte transport towards secretion by increasing intracellular Ca+ and activation of PKA...
  23. pmc The P2Y2 nucleotide receptor requires interaction with alpha v integrins to access and activate G12
    Zhongji Liao
    Department of Biochemistry, Life Sciences Center, University of Missouri Columbia, Columbia, MO 65211, USA
    J Cell Sci 120:1654-62. 2007
    ....
  24. ncbi Extracellular nucleotides induce arterial smooth muscle cell migration via osteopontin
    H Chaulet
    INSERM U441, Pessac, France
    Circ Res 89:772-8. 2001
    ..Taken together, these results suggest that extracellular nucleotides, and particularly UTP, can induce arterial SMC migration via the action of osteopontin...
  25. ncbi Nucleotide receptors involved in UTP-induced rat arterial smooth muscle cell migration
    Xavier Pillois
    INSERM U441, Pessac, France
    Circ Res 90:678-81. 2002
    ..Taken together, these results suggest a prominent role for the UTP receptor, P2Y(2), and for the UDP receptor, P2Y(6), in UTP-induced rat ASMC migration...
  26. pmc Binding of the P2Y2 nucleotide receptor to filamin A regulates migration of vascular smooth muscle cells
    Ningpu Yu
    Department of Biochemistry, University of Missouri, Columbia, MO 65211 7310, USA
    Circ Res 102:581-8. 2008
    ..Thus, this study demonstrates that P2Y(2)R/FLNa interaction selectively regulates spreading and migration of vascular SMCs...
  27. pmc UTP-bound and Apo structures of a minimal RNA uridylyltransferase
    Jason Stagno
    Department of Molecular Biology and Biochemistry, University of California, Irvine, CA 92697, USA
    J Mol Biol 366:882-99. 2007
    ..We also report a 2.4 Angstroms crystal structure of TbTUT4 with the bound 2' deoxyribonucleoside, which provides the structural basis of the enzyme's preference toward ribonucleotides...
  28. ncbi Constitutive release of ATP and evidence for major contribution of ecto-nucleotide pyrophosphatase and nucleoside diphosphokinase to extracellular nucleotide concentrations
    E R Lazarowski
    Departments of Medicine and Pharmacology, School of Medicine, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    J Biol Chem 275:31061-8. 2000
    ..In addition to the existence of an ecto-ATPase activity, our results suggest a major scavenger role of ecto-ATP pyrophosphatase and a transphosphorylating activity of nucleoside diphosphokinase...
  29. ncbi Molecular cloning and characterization of the mouse P2Y4 nucleotide receptor
    N Suarez-Huerta
    Institut de Recherche Interdisciplinaire en Biologie Humaine et Nucleaire, Universite Libre de Bruxelles, 808 Route de Lennik, Bat C 1070, Brussels, Belgium
    Eur J Pharmacol 416:197-202. 2001
    ..Thus, when compared in the same expression system, the mouse P2Y4 is closer to the rat ortholog in terms of agonist stimulation, while in terms of antagonist profile, the three P2Y4 receptor orthologs are similar...
  30. ncbi Effects of luminal flow and nucleotides on [Ca(2+)](i) in rabbit cortical collecting duct
    Craig B Woda
    Department of Pediatrics, Mount Sinai School of Medicine, New York, New York 10029 6574, USA
    Am J Physiol Renal Physiol 283:F437-46. 2002
    ..The flow-induced rise in [Ca(2+)](i) is apparently not mediated by apical P2 purinergic receptor signaling...
  31. ncbi Luminal P2Y2 receptor-mediated inhibition of Na+ absorption in isolated perfused mouse CCD
    Heiko Lehrmann
    Physiologisches Institut, Albert Ludwigs Universitat, Hermann Herder Strasse 7, 79104 Freiburg, Germany
    J Am Soc Nephrol 13:10-8. 2002
    ..Herein the effects of adenosine triphosphate (ATP) and uridine triphosphate (UTP) on electrogenic Na+ absorption in perfused CCD of mice kept on a low-NaCl diet were investigated...
  32. ncbi Secretion of ATP from Schwann cells in response to uridine triphosphate
    Guo Jun Liu
    The Neurobiology Laboratory, Department of Physiology and Institute for Biomedical Research, University of Sydney, NSW, 2006 Australia
    Eur J Neurosci 21:151-60. 2005
    The mechanisms by which uridine triphosphate (UTP) stimulates ATP release from Schwann cells cultured from the sciatic nerve were investigated using online bioluminescence techniques...
  33. pmc Thermal nociception and TRPV1 function are attenuated in mice lacking the nucleotide receptor P2Y2
    Sacha A Malin
    Department of Medicine, University of Pittsburgh, Pittsburgh, S841 Scaife Hall, 3550 Terrace Avenue, PA 15261, USA
    Pain 138:484-96. 2008
    ..These results indicate that nucleotide signaling through P2Y(2) plays a key role in thermal nociception...
  34. pmc Calcium store-mediated signaling in sustentacular cells of the mouse olfactory epithelium
    Colleen Cosgrove Hegg
    Department of Pharmacology and Toxicology, Michigan State University, East Lansing, Michigan, USA
    Glia 57:634-44. 2009
    ..Our findings indicate that sustentacular cells are not static support cells, and, like glia in the central nervous system, have complex calcium signaling...
  35. ncbi ATP and UTP excite sensory neurons and induce CREB phosphorylation through the metabotropic receptor, P2Y2
    Derek C Molliver
    Vollum Institute, Oregon Health and Science University, 3181 SW Sam Jackson Park Road, Portland, OR 97201, USA
    Eur J Neurosci 16:1850-60. 2002
    ..These findings suggest that P2Y2 contributes to the transduction of ATP-mediated sensory signalling, and may be involved in the activity-dependent regulation of nociceptor phenotype...
  36. pmc Dual coupling of heterologously-expressed rat P2Y6 nucleotide receptors to N-type Ca2+ and M-type K+ currents in rat sympathetic neurones
    A K Filippov
    Department of Pharmacology, University College London, England, UK
    Br J Pharmacol 126:1009-17. 1999
    ..The very high sensitivity to both UDP and UTP suggests that it might be preferentially activated by any locally released uridine nucleotides...
  37. ncbi Differential effects of UTP, ATP, and adenosine on ciliary activity of human nasal epithelial cells
    D M Morse
    Cystic Fibrosis/Pulmonary Research and Treatment Center, University of North Carolina, Chapel Hill, NC 27599-7248, USA
    Am J Physiol Cell Physiol 280:C1485-97. 2001
    ..Hence, ATP affects ciliary activity through P2Y(2)-R and, after an apparent ectohydrolysis to adenosine, through A(2B)AR...
  38. ncbi The P2Y agonist UTP activates cutaneous afferent fibers
    Cheryl L Stucky
    Department of Cell Biology, Neurobiology and Anatomy, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226 0509, USA
    Pain 109:36-44. 2004
    ..Furthermore, P2Y signaling may contribute to mechanotransduction in low threshold Abeta fibers under normal or pathological conditions...
  39. ncbi Uridine triphosphate (UTP) is released during cardiac ischemia
    David Erlinge
    Department of Cardiology, Lund University Hospital, S 221 85 Lund, Sweden
    Int J Cardiol 100:427-33. 2005
    Extracellular uridine triphosphate (UTP) stimulates vasodilatation, automaticity in ventricular myocytes and release of tissue-plasminogen activator (t-PA), indicating that UTP may be important in cardiac regulation...
  40. ncbi UTP inhibits Na+ absorption in wild-type and DeltaF508 CFTR-expressing human bronchial epithelia
    D C Devor
    Department of Physiology and Cell Biology, University of Pittsburgh, Pittsburgh, Pennsylvania 15261, USA
    Am J Physiol 276:C827-37. 1999
    ..Thus UTP may have a dual therapeutic effect in CF airway: 1) stimulation of a Cl- secretory response and 2) inhibition of Na+ transport...
  41. pmc Agonist-induced phosphorylation and desensitization of the P2Y2 nucleotide receptor
    Rosa V Flores
    Department of Chemistry, University of Puerto Rico, Rio Piedras Campus, San Juan, PR, USA
    Mol Cell Biochem 280:35-45. 2005
    ..Our results indicate a role for receptor phosphorylation by phorbol-insensitive protein kinases in agonist-induced desensitization of the P2Y2 nucleotide receptor...
  42. pmc Fluorescent labelling of cRNA for microarray applications
    Peter A C 't Hoen
    Center for Human and Clinical Genetics, Leiden University Medical Center, Wassenaarseweg 72, 2333 AL Leiden, The Netherlands
    Nucleic Acids Res 31:e20. 2003
    ..In conclusion, the currently developed method is an efficient, robust and inexpensive technique for fluorescent labelling of cRNA and allows sensitive detection of gene expression profiles on oligonucleotide microarrays...
  43. ncbi Effect of luminal nucleotides on Cl- secretion and Na+ absorption in distal bronchi
    S K Inglis
    Lung Membrane Transport Group, Tayside Institute of Child Health, University of Dundee, Ninewells Hospital and Medical School, UK
    Pflugers Arch 438:621-7. 1999
    ..Both effects are likely to favour increased hydration of the airway surface, and may therefore be beneficial in CF...
  44. pmc Agonist-selective, receptor-specific interaction of human P2Y receptors with beta-arrestin-1 and -2
    Carsten Hoffmann
    Institute for Pharmacology and Toxicology, Versbacher Strasse 9, D 97078 Wuerzburg, Germany
    J Biol Chem 283:30933-41. 2008
    ..This suggests that the two different agonists induce distinct active states of this receptor that show differential interactions with beta-arrestins...
  45. ncbi Intestinal inflammation increases the expression of the P2Y6 receptor on epithelial cells and the release of CXC chemokine ligand 8 by UDP
    Djordje M Grbic
    Canadian Institutes of Health Research Team on the Digestive Epithelium, Département d Anatomie et de Biologie Cellulaire, Faculte de Medecine et des Sciences de la Sante, Universite de Sherbrooke, 3001 12th Avenue North, Sherbrooke, Quebec, Canada
    J Immunol 180:2659-68. 2008
    ..These results demonstrate the emergence of extracellular nucleotide signaling in the orchestration of intestinal inflammation...
  46. pmc Characterization of P2-purinoceptors in the smooth muscle of the rat tail artery: a comparison between contractile and electrophysiological responses
    R J Evans
    Department of Physiology and Pharmacology, University of Strathclyde, Royal College, Glasgow
    Br J Pharmacol 113:853-60. 1994
    ..It is concluded that this difference may be due to the relative absence of breakdown of some of the agonists in the single cell system compared with artery rings...
  47. ncbi Uridine nucleotide selectivity of three phospholipase C-activating P2 receptors: identification of a UDP-selective, a UTP-selective, and an ATP- and UTP-specific receptor
    R A Nicholas
    Department of Pharmacology, University of North Carolina, School of Medicine, Chapel Hill 27599 7365, USA
    Mol Pharmacol 50:224-9. 1996
    ....
  48. pmc Functional interaction between native G protein-coupled purinergic receptors in Xenopus follicles
    Rogelio O Arellano
    Departamento de Neurobiologia Celular y Molecular, Instituto de Neurobiologia, Universidad Nacional Autonoma de Mexico, Boulevard Juriquilla 3001, Juriquilla Querétaro, Queretaro, Mexico
    Proc Natl Acad Sci U S A 106:16680-5. 2009
    ..We hypothesize that this property was due to direct interactions between receptors for Ado (A2 subtype) and ATP (P2Y subtype) in the Xenopus follicle...
  49. pmc Cloning and expression of a human P2U nucleotide receptor, a target for cystic fibrosis pharmacotherapy
    C E Parr
    Division of Pulmonary Diseases, University of North Carolina, Chapel Hill 27599 7020
    Proc Natl Acad Sci U S A 91:3275-9. 1994
    ..Thus, HP2U expression in the 1321N1 cell line will be useful for studying autocrine regulatory mechanisms and in screening of potential therapeutic drugs...
  50. pmc Structural basis for UTP specificity of RNA editing TUTases from Trypanosoma brucei
    Junpeng Deng
    Howard Hughes Medical Institute, University of Washington, Seattle, WA 98195, USA
    EMBO J 24:4007-17. 2005
    ..This insertion is present in all trypanosomatid TUTases. We also show that TbRET2 is essential for survival of the bloodstream form of the parasite and therefore is a potential target for drug therapy...
  51. ncbi Regulation of extracellular UTP-activated Cl- current by P2Y-PLC-PKC signaling and ATP hydrolysis in mouse ventricular myocytes
    Shintaro Yamamoto
    Department of Physiology, Saga University Faculty of Medicine, Saga, 849 8501 Japan
    J Physiol Sci 57:85-94. 2007
    ..Our results strongly suggest that I(Cl.UTP) is due to the activation of CFTR Cl- channels through Gq/11-coupled P2Y2 receptor-PLC-PKC signaling and ATP hydrolysis in mouse heart...
  52. ncbi The P2Y2 nucleotide receptor interacts with alphav integrins to activate Go and induce cell migration
    Sriparna Bagchi
    Department of Biochemistry, University of Missouri, Columbia, Missouri 65211, USA
    J Biol Chem 280:39050-7. 2005
    ..Thus, the RGD-dependent interaction between the P2Y(2)R and alpha(v) integrins is necessary for the P2Y(2)R to activate G(o) and to initiate G(o)-mediated signaling events leading to chemotaxis...
  53. ncbi P2Y nucleotide receptor interaction with alpha integrin mediates astrocyte migration
    Min Wang
    Interdisciplinary Neuroscience Program, University of Missouri Columbia, Missouri 65211, USA
    J Neurochem 95:630-40. 2005
    ..These results suggest that P2Y(2)Rs and their associated signaling pathways may be important factors regulating astrogliosis in brain disorders...
  54. pmc Involvement of uracil nucleotides in protection of cardiomyocytes from hypoxic stress
    Smadar Yitzhaki
    Faculty of Life Sciences, Bar Ilan University, Ramat Gan 52900, Israel
    Biochem Pharmacol 69:1215-23. 2005
    ....
  55. pmc Evidence for P2Y1, P2Y2, P2Y6 and atypical UTP-sensitive receptors coupled to rises in intracellular calcium in mouse cultured superior cervical ganglion neurons and glia
    Jennifer A Calvert
    Department of Cell Physiology and Pharmacology, University of Leicester, Medical Sciences Building, University Road, Leicester LE1 9HN
    Br J Pharmacol 143:525-32. 2004
    ....
  56. pmc Spreading dilatation to luminal perfusion of ATP and UTP in rat isolated small mesenteric arteries
    Polly Winter
    Department of Pharmacy and Pharmacology, University of Bath, Claverton Down, Bath BA2 7AY, UK
    J Physiol 582:335-47. 2007
    ....
  57. ncbi Ionic currents activated via purinergic receptors in the cumulus cell-enclosed mouse oocyte
    Rogelio O Arellano
    Centro de Neurobiologia, Universidad Nacional Autonoma de Mexico, Queretaro 76230, Mexico
    Biol Reprod 67:837-46. 2002
    ..Both the ion channels and the receptors seemed to be located in the cumulus cells, which transmit their corresponding electrical signals to the oocyte via gap junction channels...
  58. ncbi Human keratinocytes express multiple P2Y-receptors: evidence for functional P2Y1, P2Y2, and P2Y4 receptors
    Helen E Burrell
    Dermatology Unit, Department of Medicine, University of Liverpool, U K
    J Invest Dermatol 120:440-7. 2003
    ..These data demonstrate that multiple P2Y receptors (P2Y1, P2Y2, and P2Y4 subtypes) are differentially involved in the regulation of proliferation in human keratinocytes and therefore may be important in wound healing...
  59. pmc The stable pyrimidines UDPbetaS and UTPgammaS discriminate between the P2 receptors that mediate vascular contraction and relaxation of the rat mesenteric artery
    M Malmsjo
    Division of Experimental Vascular Research, Department of Medicine, Lund University Hospital, Lund, Sweden
    Br J Pharmacol 131:51-6. 2000
    ..Uridine diphosphate (UDP), uridine triphosphate (UTP) and ATP stimulated contraction only in high concentrations (1 - 10 mM)...
  60. ncbi Uridine-5'-triphosphate (UTP) reduces infarct size and improves rat heart function after myocardial infarct
    Smadar Yitzhaki
    The Mina and Everard Goodman Faculty of Life Sciences, Bar Ilan University, Ramat Gan 52900, Israel
    Biochem Pharmacol 72:949-55. 2006
    ..The resulting cardioprotection following UTP treatment post ischemia demonstrates a reduction in mitochondrial calcium overload, which can explain the beneficial effect of UTP...
  61. pmc ATP and UTP responses of cultured rat aortic smooth muscle cells revisited: dominance of P2Y2 receptors
    Rajendra Kumari
    Cell Signalling Laboratory, Leicester School of Pharmacy, De Montfort University, The Hawthorn Building, The Gateway, Leicester LE1 9BH, UK
    Br J Pharmacol 140:1169-76. 2003
    ..7. Taken together with the results of others, these findings indicate that the response of cultured rat VSMCs to UTP and to ATP is predominantly at the P2Y2 receptor, and that there is also a response to UDP at the P2Y6 receptor...
  62. ncbi Differential frequency dependence of P2Y1- and P2Y2- mediated Ca 2+ signaling in astrocytes
    Sami R Fam
    Programme in Brain and Behaviour, Hospital for Sick Children, University of Toronto, Toronto, Ontario, Canada M5G 1X8
    J Neurosci 23:4437-44. 2003
    ..We propose that frequency-dependent changes in ATP-mediated Ca 2+ signaling pathways may modulate astrocyte function and astrocyte-neuron signaling in the CNS...
  63. ncbi Mechanisms of secretion of ATP from cortical astrocytes triggered by uridine triphosphate
    Andrea Abdipranoto
    Department of Physiology and Institute for Biomedical Research, University of Sydney, Australia
    Neuroreport 14:2177-81. 2003
    ..Astrocytes released ATP in response to application of 10 microM uridine triphosphate, which was blocked by the non-specific purinergic receptor antagonist suramin...
  64. pmc Selective stimulation of catecholamine release from bovine adrenal chromaffin cells by an ionotropic purinergic receptor sensitive to 2-methylthio ATP
    Angelo R Tomé
    Center for Neurosciences and Cell Biology, University of Coimbra, Coimbra, Portugal
    BMC Neurosci 8:41. 2007
    ....
  65. pmc Dual role of the RNA substrate in selectivity and catalysis by terminal uridylyl transferases
    Jason Stagno
    Department of Molecular Biology and Biochemistry, University of California, Irvine, CA 92697, USA
    Proc Natl Acad Sci U S A 104:14634-9. 2007
    ..We propose a dual role for RNA substrates in TUTase-catalyzed reactions: contribution to selective incorporation of the cognate nucleoside and shaping of the catalytic metal binding site...
  66. pmc Positive inotropic effects by uridine triphosphate (UTP) and uridine diphosphate (UDP) via P2Y2 and P2Y6 receptors on cardiomyocytes and release of UTP in man during myocardial infarction
    Anna Karin Wihlborg
    Department of Cardiology, Lund University, Sweden
    Circ Res 98:970-6. 2006
    ..Thus, the extracellular pyrimidines (UTP and UDP) could be important inotropic factors involved in the development of cardiac disease...
  67. pmc Molecular cloning and characterization of rat P2Y4 nucleotide receptor
    Y D Bogdanov
    Department of Anatomy and Developmental Biology, University College London
    Br J Pharmacol 124:428-30. 1998
    ..Thus, rP2Y4 and the P2Y2 subtype appear to be structurally distinct forms of the P2U receptor (where ATP and UTP are equi-active) but can be distinguished as suramin-insensitive and suramin-sensitive P2U receptors, respectively...
  68. ncbi Half of Saccharomyces cerevisiae carbamoyl phosphate synthetase produces and channels carbamoyl phosphate to the fused aspartate transcarbamoylase domain
    V Serre
    Laboratoire de Biochimie des Signaux Régulateurs Cellulaires et Moléculaires, UMR 7631 CNRS Université Pierre et Marie Curie, 96 Bd Raspail 75006 Paris, France
    J Biol Chem 274:23794-801. 1999
    ....
  69. ncbi Regulation of vascular tone by UTP and UDP in isolated rat intrapulmonary arteries
    A Rubino
    Autonomic Neuroscience Institute, Royal Free and University College Medical School, University College London, UK
    Eur J Pharmacol 370:139-43. 1999
    ..Furthermore, the pharmacological profile of the native UTP/UDP receptor differs from that of the known P2Y2, P2Y4 and P2Y6 recombinant receptors for pyrimidine nucleotides...
  70. pmc In the presence of CTP, UTP becomes an allosteric inhibitor of aspartate transcarbamoylase
    J R Wild
    Department of Biochemistry and Biophysics, Texas A and M University, College Station 77843
    Proc Natl Acad Sci U S A 86:46-50. 1989
    ..The synergistic inhibition of ATCase by both CTP and UTP provides a satisfying logic for ensuring a balance of endogenous pyrimidine nucleotide pools...
  71. ncbi Purinergic receptors coupled to intracellular Ca2+ signals and exocytosis in rat prostate neuroendocrine cells
    Jun Hee Kim
    Department of Physiology and Center for Molecular Medicine, Sungkyunkwan University School of Medicine, Suwon, Republic of Korea
    J Biol Chem 279:27345-56. 2004
    ..From these results, we postulate that a sharp rise in [Ca(2+)](c) by the P2X-mediated Ca(2+) influx is required for exocytosis, whereas the relatively slow release of stored Ca(2+) induces endocytosis in RPNECs...
  72. ncbi Oscillatory calcium responses mediated by P2Y2 purinergic receptors in terminal Schwann cells of longitudinal lanceolate endings isolated from rat vibrissae
    Hiromi Takahashi-Iwanaga
    Laboratory of Histology and Cytology, Graduate School of Medicine, Hokkaido University, Sapporo 060 8638, Japan
    J Comp Neurol 475:416-25. 2004
    ....
  73. ncbi Pharmacological properties of P2 receptors on rat otic parasympathetic ganglion neurons
    Bei Ma
    Department of Physiology, Second Military Medical University, 800 Xiangyin Road, Shanghai, PR China
    Life Sci 83:185-91. 2008
    ..In conclusion, in rat otic ganglion neurons, P2X(2/3) heteromultimer receptors dominate, but P2X(7) and P2Y(2) or P2Y(4) receptors also play roles...
  74. ncbi Enhanced P2X7 activity in human fibroblasts from diabetic patients: a possible pathogenetic mechanism for vascular damage in diabetes
    Anna Solini
    Department of Internal Medicine, University of Pisa, Italy
    Arterioscler Thromb Vasc Biol 24:1240-5. 2004
    ..We have investigated expression and function of the P2X7 receptor in fibroblasts from healthy subjects and patients with type 2 diabetes...
  75. ncbi P2Y6 nucleotide receptors activate NF-kappaB and increase survival of osteoclasts
    Jasminka Korcok
    Canadian Institutes of Health Research Group in Skeletal Development and Remodeling, Department of Physiology and Pharmacology and Division of Oral Biology, Faculty of Medicine and Dentistry, The University of Western Ontario, London, Canada
    J Biol Chem 280:16909-15. 2005
    ..Thus, nucleotides, following their release at sites of inflammation and mechanical stimulation, can act through P2Y6 receptors to initiate NF-kappaB signaling and enhance osteoclast survival...
  76. ncbi Nucleotide-mediated mucin secretion from differentiated human bronchial epithelial cells
    Philip A Kemp
    Novartis Respiratory Research Centre, Wimblehurst Road, Horsham, West Sussex RH12 5AB, UK
    Am J Respir Cell Mol Biol 31:446-55. 2004
    ..ATP and uridine triphosphate equipotently stimulated mucin secretion consistent with mediation via P2Y2 receptor activation...
  77. ncbi P2Y2 receptor activation regulates the expression of acetylcholinesterase and acetylcholine receptor genes at vertebrate neuromuscular junctions
    Edmund K K Tung
    Department of Biology, The Hong Kong University of Science and Technology, Clear Water Bay Rd, Hong Kong SAR, China
    Mol Pharmacol 66:794-806. 2004
    ..These results indicate that ATP regulates postsynaptic gene expressions via a common pathway triggered by the activation of P2Y1 and P2Y2 receptors at the nmjs...
  78. pmc P2X7 receptors stimulate AKT phosphorylation in astrocytes
    Maria C Jacques-Silva
    Departamento de Bioquimica, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil
    Br J Pharmacol 141:1106-17. 2004
    ....
  79. ncbi Expression of P2Y nucleotide receptors and ectonucleotidases in quiescent and activated rat hepatic stellate cells
    Jonathan A Dranoff
    Yale Univ School of Medicine, Section of Digestive Diseases, 333 Cedar St LMP 1080, New Haven, CT 06520, USA
    Am J Physiol Gastrointest Liver Physiol 287:G417-24. 2004
    ..Because activation of P2Y receptors in activated HSC regulates procollagen-1 transcription, P2Y receptors may be an attractive target to prevent or treat liver fibrosis...
  80. ncbi Activation of P2 nucleotide receptors stimulates acid efflux from astrocytes
    S Jeffrey Dixon
    Department of Physiology and Pharmacology, Faculty of Medicine and Dentistry, University of Western Ontario, London, Canada
    Glia 47:367-76. 2004
    ..The resulting acidification of the extracellular fluid may serve as an intercellular signal in brain...
  81. ncbi Nucleotide regulation of the voltage-dependent nonselective cation conductance in murine colonic myocytes
    Kevin P Monaghan
    Dept of Physiology and Cell Biology, University of Nevada School of Medicine, Reno, NV 89557, USA
    Am J Physiol Cell Physiol 291:C985-94. 2006
    ..Our data suggest that ATP activates I(VNSCC) and depresses I(L) via binding of P2Y(4) receptors and stimulation of the phospholipase C/PKC pathway...
  82. ncbi P2 purinergic receptors signal to STAT3 in astrocytes: Difference in STAT3 responses to P2Y and P2X receptor activation
    K B Washburn
    Research Service 151, Miami Veterans Affairs Medical Center, Department of Pathology, University of Miami Miller School of Medicine, 1201 NW 16th Street, Miami, FL 33215, USA
    Neuroscience 142:411-23. 2006
    ..We conclude that P2 receptors stimulate STAT3 activation and suggest that P2 receptor/STAT3 signaling may play an important role in astrocyte proliferation and reactive astrogliosis...
  83. ncbi Osteoblast responses to nucleotides increase during differentiation
    Isabel R Orriss
    Department of Anatomy and Developmental Biology, University College London, London WC1E 6BT, UK
    Bone 39:300-9. 2006
    ..Taken together, these data suggest that the P2Y(2) receptor, and possibly the P2Y(4) receptor, could function as 'off-switches' for mineralized bone formation...
  84. ncbi Extracellular nucleotide signaling in adult neural stem cells: synergism with growth factor-mediated cellular proliferation
    Santosh K Mishra
    Biocenter, J W Goethe University, AK Neurochemistry, Frankfurt am Main, Germany
    Development 133:675-84. 2006
    ..Together with the in situ data, this supports the notion that extracellular nucleotides contribute to the control of adult neurogenesis...
  85. pmc Endothelium-dependent relaxation evoked by ATP and UTP in the aorta of P2Y2-deficient mice
    Pieter Jan D F Guns
    Division of Pharmacology, University of Antwerp, Universiteitsplein 1 building T2, B 2610 Wilrijk, and Laboratory of Medical Chemistry, Erasme Hospital, Belgium
    Br J Pharmacol 147:569-74. 2006
    ..But this P2Y2 receptor has apparently no major role in UTP-evoked relaxation. The vasodilator effect of UTP is probably mediated mainly by a P2Y6(like) receptor...
  86. ncbi Spontaneous oscillation and mechanically induced calcium waves in chondrocytes
    Taisuke Kono
    Department of Orthopaedics, Faculty of Medicine, Shimane University, Izumo shi, Shimane, Japan
    Cell Biochem Funct 24:103-11. 2006
    ..Thus, ATP stimulation of P2Y receptors elicits an increase in intracellular Ca(2+), triggering further release of ATP from adjacent cells, thereby expanding the Ca(2+) wave in chondrocytes...
  87. ncbi Activation-dependent trafficking of NTPDase2 in Chinese hamster ovary cells
    Srdjan M Vlajkovic
    Department of Physiology, Faculty of Medical and Health Sciences, University of Auckland, Auckland, New Zealand
    Int J Biochem Cell Biol 39:810-7. 2007
    ..The increase in surface-located NTPDase2 may reflect a regulatory mechanism counteracting excessive stimulation and desensitisation of P2 receptors...
  88. ncbi Calcium oscillations induced by ATP in human umbilical cord smooth muscle cells
    Fei Meng
    Department of Physiology, School of Medicine, University of Birmingham, Edgbaston, UK
    J Cell Physiol 213:79-87. 2007
    ..Chronic hypoxia enhanced the Ca(2+) response and altered the oscillation frequency. We suggest that these oscillations may play a role in the maintenance of umbilical blood flow during situations in which GPCR are activated...
  89. pmc Multiple P2Y receptors couple to calcium-dependent, chloride channels in smooth muscle cells of the rat pulmonary artery
    Krongkarn Chootip
    Department of Physiology and Pharmacology, University of Strathclyde, Strathclyde Institute for Biomedical Sciences, Glasgow G4 ONR, UK
    Respir Res 6:124. 2005
    ....
  90. ncbi Changes in purinergic signalling in developing and ageing rat tail artery: importance for temperature control
    Anna Wallace
    Autonomic Neuroscience Centre, Royal Free and University College Medical School, Rowland Hill Street, London NW3 2PF, UK
    Neuropharmacology 50:191-208. 2006
    ..We speculate that the dramatic changes in expression of P2 receptors in the rat tail artery, compared to the mesenteric artery, during development and ageing are related to the role of the tail artery in temperature regulation...
  91. pmc A stochastic two-dimensional model of intercellular Ca2+ wave spread in glia
    Dumitru A Iacobas
    Department of Neuroscience, Albert Einstein College of Medicine, Bronx, New York, USA
    Biophys J 90:24-41. 2006
    ..Such modeling is expected to be useful for testing phenomenological hypotheses and in understanding consequences of alteration of system components under experimental or pathological conditions...
  92. pmc Otopetrin 1 activation by purinergic nucleotides regulates intracellular calcium
    Inna Hughes
    Department of Molecular Biology and Pharmacology, Washington University School of Medicine, St Louis, MO 63110, USA
    Proc Natl Acad Sci U S A 104:12023-8. 2007
    ..In a physiologic setting, these activities could direct the formation and growth of otoconia and regulate other biomineralization processes...
  93. ncbi P2Y receptor regulation of sodium transport in human mammary epithelial cells
    So Yeong Lee
    Department of Veterinary Pharmacology, College of Veterinary Medicine, Seoul National University, Seoul, South Korea
    Am J Physiol Cell Physiol 293:C1472-80. 2007
    ....
  94. ncbi Modulation of ERK 1/2 and p38 MAPK signaling pathways by ATP in osteoblasts: involvement of mechanical stress-activated calcium influx, PKC and Src activation
    S Katz
    Departamento de Biologia, Bioquimica y Farmacia, Universidad Nacional del Sur, San Juan 670, B8000ICN Bahia Blanca, Argentina
    Int J Biochem Cell Biol 38:2082-91. 2006
    ..This effect also involves upstream mediators such as PI-PLC, PKC and Src family kinases...
  95. ncbi Distinct P2Y receptor subtypes regulate calcium signaling in human retinal pigment epithelial cells
    Victoria E Tovell
    School of Chemical Sciences and Pharmacy, University of East Anglia, Norwich, United Kingdom
    Invest Ophthalmol Vis Sci 49:350-7. 2008
    ..The purpose of this study was to investigate the expression of P2Y receptor subtypes in native and cultured human RPE cells...
  96. ncbi P2Y2 receptor desensitization on single endothelial cells
    PRISCILA SANABRIA
    Department of Physiology, Universidad Central del Caribe, Bayamon, Puerto Rico
    Endothelium 15:43-51. 2008
    ..These results suggest differential mechanisms of P2Y(2) receptor desensitization and favors heterogeneity in extracellular nucleotide activity in endothelial cells according to its vascular bed origin...
  97. ncbi P2Y2 receptor up-regulation induced by guanosine or UTP in rat brain cultured astrocytes
    P Ballerini
    Dept of Biomedical Sciences, Section of Pharmacology and Toxicology, Medical School, University G d Annunzio of Chieti Pescara, Via dei Vestini 29, 66013 Chieti, Italy
    Int J Immunopathol Pharmacol 19:293-308. 2006
    ....
  98. ncbi P2 purinoceptors regulate calcium-activated chloride and fluid transport in 31EG4 mammary epithelia
    Sasha Blaug
    Department of Molecular and Cell Biology, University of California at Berkeley, Berkeley, California 94720 3200, USA
    Am J Physiol Cell Physiol 284:C897-909. 2003
    ..This suggests that extracellular nucleotides could play a fundamental role in mammary gland paracrine signaling and the regulation of milk composition in vivo...
  99. ncbi Physiological evidence for a P2Y receptor responsive to diadenosine polyphosphates in human lung via Ca(2+) release studies in bronchial epithelial cells
    W Laubinger
    Institute of Neurobiochemistry, Otto-von-Guericke University of Magdeburg, Faculty of Medicine, Leipziger Strasse 44, D-39120, Magdeburg, Germany
    Biochem Pharmacol 61:623-9. 2001
    ....
  100. ncbi Increase of [Ca(2+)](i) via activation of ATP receptors in PC-Cl3 rat thyroid cell line
    Santo Marsigliante
    Laboratory of Physiology, Department of Biology, University of Lecce, Lecce 73100, Italy
    Cell Signal 14:61-7. 2002
    ..These data suggest the existence, in PC-Cl3 cell line, of a P2Y purinergic receptor able to increase the [Ca(2+)](i) via PLC activation, Ca(2+) store depletion, capacitative Ca(2+) entry and L-VDCC activation...
  101. ncbi ATP induces leukemia inhibitory factor mRNA in cultured rat astrocytes
    Hisashi Yamakuni
    Department of Molecular Pharmacology, Faculty of Pharmaceutical Sciences, Kyoto University, 606 8501, Kyoto, Japan
    J Neuroimmunol 129:43-50. 2002
    ..Experiments using ATP, UTP and related compounds showed the involvement of P2Y2 and P2Y4 purinoceptors in the expression induced by ATP...

Research Grants44

  1. Mechanisms of Nucleotide Release by Airway Epithelia
    Eduardo Lazarowski; Fiscal Year: 2006
    ..Delineation of nucleotide-releasing mechanisms will provide new possibilities to improve MCC function in chronic lung diseases characterized by mucin-obstructed and inflamed airways. [unreadable] [unreadable]..
  2. Properties of Ion Channels that Control Secretion
    JOHN ENYEART; Fiscal Year: 2008
    ..abstract_text> ..
  3. Mechanisms of antibiotic action in Neisseria gonorrhoeae.
    Robert A Nicholas; Fiscal Year: 2010
    ..Together, these projects utilize genetic, biochemical, biophysical, and proteomics approaches to generate new insights into the mechanisms of antibiotic action in N. gonorrhoeae. ..
  4. RNA Uridylytransferases of Trypanosomes
    Ruslan Aphasizhev; Fiscal Year: 2009
    ..Sequences of the newly identified UTP-specific transferases will be used to establish characteristic protein motifs for this family of enzymes. ..
  5. Trafficking/signaling of P2Y receptors in polarized epithelial cells
    Robert A Nicholas; Fiscal Year: 2011
    ..Our studies will provide novel and important information on the cellular mechanisms of P2Y receptor targeting and the regulation of P2Y receptor signaling activity in epithelial cells. ..
  6. Glial Signaling:Interplay of Connexins and P2 Receptors
    Eliana Scemes; Fiscal Year: 2006
    ....
  7. "Connexin-Dependent Transcriptomic Networks in Controlling the Heart Rhythm"
    DUMITRU ANDREI IACOBAS; Fiscal Year: 2010
    ..Our project has the unique feature of describing and quantifying the Cx40- and Cx43-dependent Gene Regulatory Networks of the heart rhythm, with the long term goal to open novel therapeutic horizons in cardiology. ..
  8. The CXCR4-SDF-1 Axis in Metastatic Rhabdomyosarcoma
    Mariusz Ratajczak; Fiscal Year: 2009
    ..This work will be of relevance for designing strategies to block the SDF-1-CXCR4 axis to treat CXCR4-positive cancers. ..
  9. NTPDase2 expression and function in the liver
    JONATHAN DRANOFF; Fiscal Year: 2005
    ..The experiments proposed in these specific aims will provide the basis of several new lines of liver disease research and should provide novel therapeutic targets for treatment of cholestatic and fibrotic liver disease. ..
  10. SHORT TERM TRAINING FOR MINORITY STUDENTS
    Sharon Rounds; Fiscal Year: 2006
    ..We present results of our previous experience showing that of students trained thus far, 45 percent have gone on to MD, PhD, or MD/PhD training programs. ..
  11. Regulation of Airway Goblet Cell Mucin Secretion
    C Davis; Fiscal Year: 2007
    ..abstract_text> ..
  12. Regulation of Nucleoside Transporters by Protein Kinases
    Lee Graves; Fiscal Year: 2007
    ..Aim #2. Elucidate the promoter elements necessary for the expression of ENT1 and ENT2. Aim #3. Examine the specific roles of ENTs in growth, differentiation and chemosensitization . ..
  13. PSYCHOPHARMACOLOGICAL EFFECTS OF EXOGENOUS CHOLINE
    Richard Wurtman; Fiscal Year: 2008
    ..abstract_text> ..
  14. Engineering a CFTR Deficient Porcine Model of CF
    SCOTT O GRADY; Fiscal Year: 2007
    ..abstract_text> ..
  15. Small GTPases and Lung Endothelial Apoptosis
    Sharon Rounds; Fiscal Year: 2009
    ..In addition, understanding of apoptosis may provide clues to treatment of lung diseases dependent upon abnormal endothelial proliferation, such as Pulmonary Arterial Hypertension and cancer. ..
  16. The function of RhoBTB proteins
    STEPHEN PAUL SOLTOFF; Fiscal Year: 2010
    ..The results of these studies may reveal a new pathway involved in the development of cancer and advance our understanding of the basic biology of transformation. ..
  17. THERMODYNAMICS OF BIOCHEMICAL SYSTEMS
    ROBERT ALBERTY; Fiscal Year: 2005
    ..This quantitative treatment of the literature data on biochemical reactions will uncover errors because deltafG'0 values can be determined by use of two or more biochemical reactions, which should give the same answer. ..
  18. Role of DEG/ENaC in the renal myogenic response
    NIKKI JERNIGAN; Fiscal Year: 2005
    ..abstract_text> ..
  19. P2T PURINERGIC RECEPTORS
    JOSE BOYER; Fiscal Year: 2004
    ..This information should prove useful in the development of new biochemical and pharmacological tools with potential use in the treatment of coagulation-related diseases. ..
  20. CALCIUM RECEPTORS AND PROLIFERATIVE SIGNALS
    Karin Rodland; Fiscal Year: 2001
    ..These aims will be accomplished through experiments using specific inhibitors and dominant negative mutants of the key participants in the hypothesized signaling pathway ..
  21. CELLULAR AND MOLECULAR BASIS OF HIV BASED THROMBOCYTOPEN
    Mariusz Ratajczak; Fiscal Year: 2002
    ..In toto, the studies proposed in this grant will increase the knowledge about pathogenesis of AIDS associated thrombocytopenia and may lead to development of new strategies for its treatment of prevention. (End of Abstract) ..
  22. IDENTIFICATION OF CA2+ REGULATED APICAL CL- CHANNELS
    Sherif Gabriel; Fiscal Year: 2003
    ..A complete characterization of Cl-A will lead to the genesis of new therapies for CF disease that would circumvent a defective CFTR. ..
  23. NEURAL AND ENDOCRINE REGULATION OF BRAIN AND BEHAVIOR
    Richard Wurtman; Fiscal Year: 2003
    ..abstract_text> ..
  24. REGULATION OF CARBAMYL PHOSPH SYNTHETASES BY MAP KINASE
    Lee Graves; Fiscal Year: 2002
    ..Eventually with the design of potent and selective inhibitors of growth factor-activated kinase signaling pathways, novel strategies for the regulation of nucleotide metabolism and cell growth may be achieved. ..
  25. CALCIUM SIGNALING AND OVARIAN CANCER
    Karin Rodland; Fiscal Year: 2001
    ....
  26. Laser Scanning Confocal Microscope Research/Education
    Michael Nathanson; Fiscal Year: 2002
    ..abstract_text> ..
  27. NICOTINIC RECEPTORS IN MAMMALIAN INTRACARDIAC NEURONS
    Javier Cuevas; Fiscal Year: 2001
    ..An understanding of the properties and physiological role of the receptors is necessary to comprehend neural control of the heart. ..
  28. Identification of a new protein that binds Src and PKC
    STEPHEN SOLTOFF; Fiscal Year: 2004
    ..Future efforts will determine if this new protein alters cell morphology, tight junction formation, or other functions in salivary cells. ..
  29. Migraine Headache Treatment by Drug Aerosol Inhalation
    Joshua Rabinowitz; Fiscal Year: 2004
    ..Accomplishment of the goals of Phase II will lead directly to human clinical testing of a commercially viable device that could improve the lives of millions of people every year. ..
  30. BRAIN GALANIN RECEPTORS AND EPILEPSY
    Andrey Mazarati; Fiscal Year: 2006
    ..abstract_text> ..
  31. NEURAL BASIS OF BEHAVIORAL SENSITIZATION
    Terry Robinson; Fiscal Year: 2001
    ..In summary, these studies should provide new insights into how drug-environment interactions can so dramatically alter drug effects, including liability to forms of drug-induced plasticity that may contribute to addiction. ..
  32. Regulation of Bile Ductular Proliferation
    JONATHAN DRANOFF; Fiscal Year: 2009
    ..We anticipate that the results of these proposed experiments will lead to novel pharmacologic approaches for the regulation of bile ductular proliferation in biliary cirrhosis. ..
  33. Metabolomics of the Virus-host Cell Interaction
    Joshua D Rabinowitz; Fiscal Year: 2010
    ..Such pathways, once identified, will be attractive new targets for antiviral therapy. ..
  34. Purinergic signaling in endothelial cells.
    Elzbieta Kaczmarek; Fiscal Year: 2005
    ..abstract_text> ..
  35. Injury-Evoked Regeneration Mechanism in Olfactory System
    Colleen Hegg; Fiscal Year: 2008
    ..Agents that modify the effects of purinergics and purinergic receptors are readily available and could therapeutically modulate cell proliferation and differentiation, in the olfactory system and in the CNS. ..
  36. Influence of drugs of abuse on neuronal morphology
    Terry Robinson; Fiscal Year: 2005
    ....
  37. GENETICS OF FAMILIAL FOCAL SEGMENTAL GLOMERULOSCLEROSIS
    Michelle Winn; Fiscal Year: 2005
    ..New mutations found in the genes known to cause FSGS will enhance our understanding of the genetics and biology of thi., disorder, takin 9 advantage of our entire resource of families. ..
  38. PARACRINE REGULATION OF BILE DUCT SECRETION BY ATP
    JONATHAN DRANOFF; Fiscal Year: 2004
    ..The Yale School of Medicine Department of Internal Medicine has pledged that the candidate will have extensive protected research time and excellent resources for the duration of the award. ..
  39. P2Y2R MEDIATED IMMUNE RESPONSES IN SALIVARY GLAND DYSFUNCTION
    Olga Baker; Fiscal Year: 2008
    ..These studies may lead to better therapeutic strategies for minimizing autoimmune-associated dysfunction of salivary gland that contributes to xerostomia in patients with SS. ..
  40. AUTOSOMAL DOMINANT FOCAL SEGMENTAL GLOMERULOSCLEROSIS
    Michelle Winn; Fiscal Year: 2004
    ..Information derived from this research will also allow a better understanding of the pathogenesis of nonhereditary forms of FSGS and may provide insights regarding management of the more common idiopathic forms of this disorder. ..
  41. Purinergic Receptors in Olfactory System
    Colleen Hegg; Fiscal Year: 2003
    ..Because ATP is released during neurotransmission and by noxious stimuli, these studies may elucidate mechanisms for the observed reduction in olfactory sensitivity during exposure to noxious agents. ..
  42. P2U(P2Y2)-PURINOCEPTOR AND WATER TRANSPORT IN RAT KIDNEY
    BELLAMKONDA KISHORE; Fiscal Year: 2004
    ..Successful completion of these studies will lead to significant insights into the AVP-independent mechanisms of regulation of collecting duct water permeability. ..
  43. Leak potassium channels in chemosensitive RTN neurons
    DANIEL MULKEY; Fiscal Year: 2006
    ..Results from these experiments will determine if TASK currents are present in pH-sensitive RTN neurons and provide insight into the cellular mechanism of mechanism chemoreception. [unreadable] [unreadable]..
  44. Gordon Conference on Catecholamines (2003)
    Terry Robinson; Fiscal Year: 2003
    ....