guanosine triphosphate

Summary

Summary: Guanosine 5'-(tetrahydrogen triphosphate). A guanine nucleotide containing three phosphate groups esterified to the sugar moiety.

Top Publications

  1. ncbi The Ras-RasGAP complex: structural basis for GTPase activation and its loss in oncogenic Ras mutants
    K Scheffzek
    Max Planck Institut fur molekulare Physiologie, Abteilung Strukturelle Biologie, Rheinlanddamm 201, 44139 Dortmund, Germany
    Science 277:333-8. 1997
  2. ncbi The guanine nucleotide-binding switch in three dimensions
    I R Vetter
    , 44227 Dortmund, Germany
    Science 294:1299-304. 2001
  3. ncbi Spontaneous cell polarization through actomyosin-based delivery of the Cdc42 GTPase
    Roland Wedlich-Soldner
    Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
    Science 299:1231-5. 2003
  4. ncbi Sar1p N-terminal helix initiates membrane curvature and completes the fission of a COPII vesicle
    Marcus C S Lee
    Department of Molecular and Cell Biology and Howard Hughes Medical Institute, University of California, Berkeley, California 94702, USA
    Cell 122:605-17. 2005
  5. ncbi TBC-domain GAPs for Rab GTPases accelerate GTP hydrolysis by a dual-finger mechanism
    Xiaojing Pan
    Program in Molecular Medicine and Department of Biochemistry and Molecular Pharmacology, UMass Medical School, Two Biotech, 373 Plantation Street, Worcester, Massachusetts 01605, USA
    Nature 442:303-6. 2006
  6. ncbi Kinase activity of mutant LRRK2 mediates neuronal toxicity
    Wanli W Smith
    Department of Psychiatry, Division of Neurobiology, Johns Hopkins University School of Medicine, CMSC 8 121, 600 North Wolfe Street, Baltimore, Maryland 21287, USA
    Nat Neurosci 9:1231-3. 2006
  7. ncbi It takes two to tango: regulation of G proteins by dimerization
    Raphael Gasper
    Max Planck Institut fur molekulare Physiologie, Abteilung Strukturelle Biologie, Dortmund, Germany
    Nat Rev Mol Cell Biol 10:423-9. 2009
  8. ncbi A novel Rab5 GDP/GTP exchange factor complexed to Rabaptin-5 links nucleotide exchange to effector recruitment and function
    H Horiuchi
    European Molecular Biology Laboratory, Heidelberg, Germany
    Cell 90:1149-59. 1997
  9. ncbi In vitro reconstitution of eukaryotic translation reveals cooperativity between release factors eRF1 and eRF3
    Elena Z Alkalaeva
    Department of Microbiology and Immunology, SUNY Downstate Medical Center, Brooklyn, NY 11203, USA
    Cell 125:1125-36. 2006
  10. pmc Identification of different roles for RanGDP and RanGTP in nuclear protein import
    D Gorlich
    Zentrum fur Molekulare Biologie der Universitat Heidelberg, Germany
    EMBO J 15:5584-94. 1996

Detail Information

Publications362 found, 100 shown here

  1. ncbi The Ras-RasGAP complex: structural basis for GTPase activation and its loss in oncogenic Ras mutants
    K Scheffzek
    Max Planck Institut fur molekulare Physiologie, Abteilung Strukturelle Biologie, Rheinlanddamm 201, 44139 Dortmund, Germany
    Science 277:333-8. 1997
    ..Glycine-12 in the transition state mimic is within van der Waals distance of both arginine-789 of GAP-334 and glutamine-61 of Ras, and even its mutation to alanine would disturb the arrangements of residues in the transition state...
  2. ncbi The guanine nucleotide-binding switch in three dimensions
    I R Vetter
    , 44227 Dortmund, Germany
    Science 294:1299-304. 2001
    ..as molecular switches and timers that cycle between inactive guanosine diphosphate (GDP)-bound and active guanosine triphosphate (GTP)-bound states...
  3. ncbi Spontaneous cell polarization through actomyosin-based delivery of the Cdc42 GTPase
    Roland Wedlich-Soldner
    Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
    Science 299:1231-5. 2003
    ....
  4. ncbi Sar1p N-terminal helix initiates membrane curvature and completes the fission of a COPII vesicle
    Marcus C S Lee
    Department of Molecular and Cell Biology and Howard Hughes Medical Institute, University of California, Berkeley, California 94702, USA
    Cell 122:605-17. 2005
    ..Thus, the initiation of vesicle budding by Sar1p couples the generation of membrane curvature with coat-protein assembly and cargo capture...
  5. ncbi TBC-domain GAPs for Rab GTPases accelerate GTP hydrolysis by a dual-finger mechanism
    Xiaojing Pan
    Program in Molecular Medicine and Department of Biochemistry and Molecular Pharmacology, UMass Medical School, Two Biotech, 373 Plantation Street, Worcester, Massachusetts 01605, USA
    Nature 442:303-6. 2006
    ..Strong conservation of both catalytic fingers indicates that most TBC-domain GAPs may accelerate GTP hydrolysis by a similar dual-finger mechanism...
  6. ncbi Kinase activity of mutant LRRK2 mediates neuronal toxicity
    Wanli W Smith
    Department of Psychiatry, Division of Neurobiology, Johns Hopkins University School of Medicine, CMSC 8 121, 600 North Wolfe Street, Baltimore, Maryland 21287, USA
    Nat Neurosci 9:1231-3. 2006
    ..These data elucidate the pathogenesis of LRRK2-linked Parkinson disease, potentially illuminate mechanisms of sporadic Parkinson disease and suggest therapeutic targets...
  7. ncbi It takes two to tango: regulation of G proteins by dimerization
    Raphael Gasper
    Max Planck Institut fur molekulare Physiologie, Abteilung Strukturelle Biologie, Dortmund, Germany
    Nat Rev Mol Cell Biol 10:423-9. 2009
    ..We propose that the juxtaposition of the G domains of two monomers across the GTP-binding sites activates the biological function of these proteins and the GTPase reaction...
  8. ncbi A novel Rab5 GDP/GTP exchange factor complexed to Rabaptin-5 links nucleotide exchange to effector recruitment and function
    H Horiuchi
    European Molecular Biology Laboratory, Heidelberg, Germany
    Cell 90:1149-59. 1997
    ..This demonstrates that a soluble exchange factor coupled to a Rab effector translocates from cytosol to the membrane, where the complex stabilizes the GTPase in the active state...
  9. ncbi In vitro reconstitution of eukaryotic translation reveals cooperativity between release factors eRF1 and eRF3
    Elena Z Alkalaeva
    Department of Microbiology and Immunology, SUNY Downstate Medical Center, Brooklyn, NY 11203, USA
    Cell 125:1125-36. 2006
    ..Cooperativity between eRF1 and eRF3 required the eRF3 binding C-terminal domain of eRF1...
  10. pmc Identification of different roles for RanGDP and RanGTP in nuclear protein import
    D Gorlich
    Zentrum fur Molekulare Biologie der Universitat Heidelberg, Germany
    EMBO J 15:5584-94. 1996
    ..Thus, binding of nucleoplasmic RanGTP to importin-beta probably triggers termination, i.e. the dissociation of importin-alpha from importin-beta and the subsequent release of the import substrate into the nucleoplasm...
  11. pmc Termination of translation in eukaryotes is governed by two interacting polypeptide chain release factors, eRF1 and eRF3
    G Zhouravleva
    Departement de Biologie et Genetique du Developpement, CNRS URA 256, Universite de Rennes, France
    EMBO J 14:4065-72. 1995
    ..It is proposed that a quaternary complex composed of eRF1, eRF3, GTP and a stop codon of the mRNA is involved in termination of polypeptide synthesis in ribosomes...
  12. ncbi Assembly dynamics of microtubules at molecular resolution
    Jacob W J Kerssemakers
    Foundation for Fundamental Research on Matter FOM Institute for Atomic and Molecular Physics AMOLF, Kruislaan 407, 1098 SJ Amsterdam, The Netherlands
    Nature 442:709-12. 2006
    ....
  13. ncbi Hydrolysis of GTP by elongation factor G drives tRNA movement on the ribosome
    M V Rodnina
    Institute of Molecular Biology, University of Witten Herdecke, Germany
    Nature 385:37-41. 1997
    ..By coupling the free energy of GTP hydrolysis to translocation, EF-G serves as a motor protein to drive the directional movement of transfer and messenger RNAs on the ribosome...
  14. ncbi An elongation factor G-induced ribosome rearrangement precedes tRNA-mRNA translocation
    Andreas Savelsbergh
    Institute of Molecular Biology, University of Witten Herdecke, 58448 Witten, Germany
    Mol Cell 11:1517-23. 2003
    ..These results indicate that the energy of GTP hydrolysis is utilized to promote the ribosome rearrangement and to bias spontaneous fluctuations within the ribosome-EF-G complex toward unidirectional movement of mRNA and tRNA...
  15. pmc Dom34:Hbs1 promotes subunit dissociation and peptidyl-tRNA drop-off to initiate no-go decay
    Christopher J Shoemaker
    Howard Hughes Medical Institute and Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Science 330:369-72. 2010
    ....
  16. pmc The mechanism for activation of GTP hydrolysis on the ribosome
    Rebecca M Voorhees
    MRC Laboratory of Molecular Biology, Cambridge CB2 0QH, UK
    Science 330:835-8. 2010
    ..The structure suggests a universal mechanism for GTPase activation and hydrolysis in translational GTPases on the ribosome...
  17. pmc Cryo-EM structure of the mammalian eukaryotic release factor eRF1-eRF3-associated termination complex
    Derek Taylor
    Department of Pharmacology, School of Medicine, Case Western Reserve University, Cleveland, OH 44106, USA
    Proc Natl Acad Sci U S A 109:18413-8. 2012
    ..Our structure provides mechanistic insight into the coordination between GTP hydrolysis by eRF3 and subsequent peptide release by eRF1...
  18. ncbi GAIP and RGS4 are GTPase-activating proteins for the Gi subfamily of G protein alpha subunits
    D M Berman
    Department of Pharmacology University of Texas Southwestern Medical Center Dallas 75235, USA
    Cell 86:445-52. 1996
    ..RGS4 activates the GTPase activity of certain Gi alpha 1 mutants (e.g., R178C), but not others (e.g., Q204L). The GAP activity of RGS proteins is consistent with their proposed role as negative regulators of G protein-mediated signaling...
  19. ncbi Functional characterization of Rab7 mutant proteins associated with Charcot-Marie-Tooth type 2B disease
    Maria Rita Spinosa
    Dipartimento di Scienze e Tecnologie Biologiche ed Ambientali, Universita del Salento, 73100 Lecce, Italy
    J Neurosci 28:1640-8. 2008
    ..Altogether, these data demonstrate that all tested CMT2B-associated Rab7 mutations are mechanistically similar, suggesting that activated forms of the Rab7 are responsible for CMT2B disease...
  20. pmc LRRK2 kinase activity is dependent on LRRK2 GTP binding capacity but independent of LRRK2 GTP binding
    Jean Marc Taymans
    Laboratory for Neurobiology and Gene Therapy, Katholieke Universiteit Leuven, Leuven, Belgium
    PLoS ONE 6:e23207. 2011
    ..We conclude that while kinase activity of LRRK2 requires an intact ROC-GTPase domain, it is independent of GDP or GTP binding to ROC...
  21. pmc Rheb GTPase is a direct target of TSC2 GAP activity and regulates mTOR signaling
    Ken Inoki
    Department of Biological Chemistry, University of Michigan Medical School, Ann Arbor, Michigan 48109, USA
    Genes Dev 17:1829-34. 2003
    ..Our data demonstrate that Rheb acts downstream of TSC1/TSC2 and upstream of mTOR to regulate cell growth...
  22. ncbi Cooperation between mDia1 and ROCK in Rho-induced actin reorganization
    N Watanabe
    Department of Pharmacology, Kyoto University Faculty of Medicine, Japan
    Nat Cell Biol 1:136-43. 1999
    ..Thus Rho may induce the formation of different actin structures affected by the balance between mDia1 and ROCK signalling...
  23. pmc Membrane localization of LRRK2 is associated with increased formation of the highly active LRRK2 dimer and changes in its phosphorylation
    Zdenek Berger
    Center for Neurologic Diseases, Department of Neurology, Brigham and Women s Hospital, Boston, Massachusetts 02115, USA
    Biochemistry 49:5511-23. 2010
    ..These findings may have implications for the sites of LRRK2 function within the cell, the identification and localization of bona fide LRRK2 substrates, and efforts to design small molecule inhibitors of LRRK2...
  24. ncbi Microtubule polymerization dynamics
    A Desai
    Department of Biochemistry and Biophysics, University of California, San Francisco 94143, USA
    Annu Rev Cell Dev Biol 13:83-117. 1997
    ..In this review, we first describe progress toward understanding the mechanism of dynamic instability of pure tubulin and then discuss the function and regulation of microtubule dynamic instability in living cells...
  25. pmc Bacillus subtilis CodY represses early-stationary-phase genes by sensing GTP levels
    M Ratnayake-Lecamwasam
    Tufts University School of Medicine, Department of Molecular Biology and Microbiology, Boston, Massachusetts 02111, USA
    Genes Dev 15:1093-103. 2001
    ....
  26. pmc Dynamic assembly of FtsZ regulated by GTP hydrolysis
    A Mukherjee
    Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City 66160, USA
    EMBO J 17:462-9. 1998
    ..Thus, FtsZ polymerization, like that of tubulin, is dynamic and regulated by GTP hydrolysis. These results provide the basis for the dynamics of the Z ring and favor a model in which the Z ring is formed by a nucleation event...
  27. ncbi Nup358, a cytoplasmically exposed nucleoporin with peptide repeats, Ran-GTP binding sites, zinc fingers, a cyclophilin A homologous domain, and a leucine-rich region
    J Wu
    Laboratory of Cell Biology, Howard Hughes Medical Institute, Rockefeller University, New York, New York 10021, USA
    J Biol Chem 270:14209-13. 1995
    ..Thus, Nup358 is the first nucleoporin shown to contain binding sites for two of three soluble nuclear transport factors so far isolated, namely karyopherin and Ran-GTP...
  28. pmc Molecular dynamics of EF-G during translocation
    Wen Li
    Department of Biochemistry and Molecular Biophysics, Columbia University, New York, New York 10032, USA
    Proteins 79:1478-86. 2011
    ....
  29. ncbi Dynamin GTPase domain mutants that differentially affect GTP binding, GTP hydrolysis, and clathrin-mediated endocytosis
    Byeong Doo Song
    Department of Cell Biology, The Scripps Research Institute, La Jolla, California 92037, USA
    J Biol Chem 279:40431-6. 2004
    ....
  30. pmc Mapping the nucleotide and isoform-dependent structural and dynamical features of Ras proteins
    Alemayehu A Gorfe
    Department of Chemistry and Biochemistry and Center for Theoretical Biological Physics, University of California San Diego, La Jolla, CA 92093 0365, USA
    Structure 16:885-96. 2008
    ..We identified a number of isoform-specific, long-range side chain interactions that define unique pathways of communication between the nucleotide binding site and the C terminus...
  31. pmc The lattice as allosteric effector: structural studies of alphabeta- and gamma-tubulin clarify the role of GTP in microtubule assembly
    Luke M Rice
    Department of Biochemistry and Biophysics and Howard Hughes Medical Institute, University of California, San Francisco, CA 94158 2517, USA
    Proc Natl Acad Sci U S A 105:5378-83. 2008
    ..These data provide new insights into the molecular origins of dynamic microtubule behavior...
  32. ncbi Structure of the alpha beta tubulin dimer by electron crystallography
    E Nogales
    Life Science Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720, USA
    Nature 391:199-203. 1998
    ....
  33. ncbi The GTPase superfamily: a conserved switch for diverse cell functions
    H R Bourne
    Department of Pharmacology, University of California, San Francisco 94143
    Nature 348:125-32. 1990
    ....
  34. ncbi The GTPase superfamily: conserved structure and molecular mechanism
    H R Bourne
    Department of Pharmacology, University of California, San Francisco 94143 0450
    Nature 349:117-27. 1991
    ....
  35. ncbi GTPase activation of elongation factors Tu and G on the ribosome
    Dagmar Mohr
    Institute of Physical Biochemistry, University of Witten Herdecke, Stockumer Strasse 10, D 58448 Witten, Germany
    Biochemistry 41:12520-8. 2002
    ..This effect appears to be augmented by interactions of other structural elements of the large ribosomal subunit with the switch regions of the factors...
  36. ncbi GTP binding is essential to the protein kinase activity of LRRK2, a causative gene product for familial Parkinson's disease
    Genta Ito
    Department of Neuropathology and Neuroscience, Graduate School of Pharmaceutical Sciences, University of Tokyo, 7 3 1 Hongo, Bunkyoku, Tokyo, 113 0033 Japan
    Biochemistry 46:1380-8. 2007
    ..These data suggest that binding of GTP to the ROC domain regulates the kinase activity of LRRK2 as well as its phosphorylation by other kinase(s)...
  37. ncbi The GTP-binding protein YlqF participates in the late step of 50 S ribosomal subunit assembly in Bacillus subtilis
    Yoshitaka Matsuo
    Department of Bioinformatics and Genomics, Graduate School of Information Science, Nara Institute of Science and Technology, 8916 5, Takayama, Ikoma, Nara 630 0101, Japan
    J Biol Chem 281:8110-7. 2006
    ..Our results collectively indicate that YlqF will organize the late step of 50 S ribosomal subunit assembly...
  38. ncbi Crystal structure and functional analysis of the eukaryotic class II release factor eRF3 from S. pombe
    Chunguang Kong
    Laboratory of Macromolecular Structure, Institute of Molecular and Cell Biology, 30 Medical Drive, Singapore 117609, Japan
    Mol Cell 14:233-45. 2004
    ..The N-terminal extension, rich in acidic amino acids, blocks the proposed eRF1 binding site, potentially regulating eRF1 binding to eRF3 in a competitive manner...
  39. pmc Nutritional control of elongation of DNA replication by (p)ppGpp
    Jue D Wang
    Department of Biology, Building 68 530, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Cell 128:865-75. 2007
    ..This control may be important for cells to maintain genomic integrity...
  40. pmc The R1441C mutation of LRRK2 disrupts GTP hydrolysis
    Patrick A Lewis
    Laboratory of Neurogenetics, National Institute on Aging, 35 Convent Drive, Bethesda, MD 20892 3707, USA
    Biochem Biophys Res Commun 357:668-71. 2007
    ..We show that LRRK2 immunoprecipitated from cells has a detectable GTPase activity that is disrupted by a familial mutation associated with PD located within the GTPase domain, R1441C...
  41. pmc Conserved bipartite motifs in yeast eIF5 and eIF2Bepsilon, GTPase-activating and GDP-GTP exchange factors in translation initiation, mediate binding to their common substrate eIF2
    K Asano
    Laboratory of Eukaryotic Gene Regulation, National Institute of Child Health and Human Development, NIH, Bethesda, MD 20892, USA
    EMBO J 18:1673-88. 1999
    ..Thus, the bipartite motif in eIF5 appears to be multifunctional, stimulating its recruitment to the 40S pre-initiation complex through interaction with eIF3 in addition to binding of its substrate eIF2...
  42. pmc Regulation of dynamic polarity switching in bacteria by a Ras-like G-protein and its cognate GAP
    Simone Leonardy
    Department of Ecophysiology, Max Planck Institute for Terrestrial Microbiology, Marburg, Germany
    EMBO J 29:2276-89. 2010
    ..Thus, the function of Ras-like G-proteins and their GAPs in regulating cell polarity is found not only in eukaryotes, but also conserved in bacteria...
  43. ncbi Structure of RGS4 bound to AlF4--activated G(i alpha1): stabilization of the transition state for GTP hydrolysis
    J J Tesmer
    Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas 75235, USA
    Cell 89:251-61. 1997
    ..The binding site for RGS4 on G(i alpha1) is also consistent with the activity of RGS proteins as antagonists of G(alpha) effectors...
  44. ncbi The Semaphorin 4D receptor Plexin-B1 is a GTPase activating protein for R-Ras
    Izumi Oinuma
    Laboratory of Molecular Neurobiology, Graduate School of Biostudies, Kyoto University, Sakyo ku, Kyoto 606 8502, Japan
    Science 305:862-5. 2004
    ..Thus, Plexin-B1 mediates Sema4D-induced repulsive axon guidance signaling by acting as a GTPase activating protein for R-Ras...
  45. ncbi Conformational changes of the small ribosomal subunit during elongation factor G-dependent tRNA-mRNA translocation
    Frank Peske
    Institute of Molecular Biology, University of Witten Herdecke, 58448 Witten, Germany
    J Mol Biol 343:1183-94. 2004
    ....
  46. ncbi A continuous, regenerative coupled GTPase assay for dynamin-related proteins
    Elena Ingerman
    Section of Molecular and Cellular Biology, Center of Genetics and Development, University of California, Davis, USA
    Methods Enzymol 404:611-9. 2005
    ..We describe a simple, continuous, coupled GTP regenerating assay that tackles the limitations of the fixed time point assays and can be used for the kinetic analysis of DRP GTP hydrolysis under unassembled and assembled conditions...
  47. pmc Reverse translocation of tRNA in the ribosome
    Shinichiro Shoji
    Department of Microbiology, The Ohio State University, Columbus, Ohio 43210, USA
    Mol Cell 24:931-42. 2006
    ..e., direct movement of the tRNAs from the E and P sites to the P and A sites, respectively). These findings have important implications for the energetics of translocation...
  48. pmc Direct regulation of GTP homeostasis by (p)ppGpp: a critical component of viability and stress resistance
    Allison Kriel
    Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX 77030, USA
    Mol Cell 48:231-41. 2012
    ....
  49. ncbi Human OLA1 defines an ATPase subfamily in the Obg family of GTP-binding proteins
    Roland Koller-Eichhorn
    Institute of Biochemistry, ETH Zurich, 8093 Zurich, Switzerland
    J Biol Chem 282:19928-37. 2007
    ....
  50. pmc RNA ligase RtcB splices 3'-phosphate and 5'-OH ends via covalent RtcB-(histidinyl)-GMP and polynucleotide-(3')pp(5')G intermediates
    Anupam K Chakravarty
    Molecular Biology Program, Sloan Kettering Institute, New York, NY 10065, USA
    Proc Natl Acad Sci U S A 109:6072-7. 2012
    ..RtcB is structurally sui generis, and its chemical mechanism is unique. The wide distribution of RtcB proteins in bacteria, archaea, and metazoa raises the prospect of an alternative enzymology based on covalently activated 3' ends...
  51. ncbi Cdc42 interacts with the exocyst and regulates polarized secretion
    X Zhang
    Department of Biology, University of Pennsylvania, Philadelphia, PA 19104, USA
    J Biol Chem 276:46745-50. 2001
    ..These results have revealed a role for Cdc42 in exocytosis. We propose that Cdc42 coordinates the vesicle docking machinery and the actin cytoskeleton for polarized secretion...
  52. ncbi Crystal structure of intact elongation factor EF-Tu from Escherichia coli in GDP conformation at 2.05 A resolution
    H Song
    School of Biochemistry and Molecular Biology, University of Leeds, Leeds, LS2 9JT, UK
    J Mol Biol 285:1245-56. 1999
    ..Comparisons of the Mg2+ binding site with other guanine nucleotide binding proteins in GDP-bound forms show that the Mg2+ co-ordination patterns are well preserved among these structures...
  53. pmc Signal sequence-independent SRP-SR complex formation at the membrane suggests an alternative targeting pathway within the SRP cycle
    David Braig
    Institut fur Biochemie und Molekularbiologie, ZBMZ, 79104 Freiburg, Germany
    Mol Biol Cell 22:2309-23. 2011
    ..Our findings reveal a remarkable flexibility of SRP-dependent protein targeting, as they indicate that substrate recognition can occur either in the cytosol via ribosome-bound SRP or at the membrane via a preassembled FtsY-SRP complex...
  54. pmc Crm1p mediates regulated nuclear export of a yeast AP-1-like transcription factor
    C Yan
    W M Keck Institute for Cellular Visualization, Rosenstiel Center and Department of Biology, MS 029, Brandeis University, 415 South Street, Waltham, MA 02453, USA
    EMBO J 17:7416-29. 1998
    ..Thus, disassembly of export complexes in response to RanGTP hydrolysis may be required for release of substrate from a terminal binding site at the nuclear pore complex (NPC)...
  55. ncbi Crystal structure of ARF1*Sec7 complexed with Brefeldin A and its implications for the guanine nucleotide exchange mechanism
    Elena Mossessova
    Howard Hughes Medical Institute, Structural Biology Program, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, NY 10021, USA
    Mol Cell 12:1403-11. 2003
    ..Thus, the Sec7 domain may act as a dual catalyst, facilitating both nucleotide release and conformational switching on ARF proteins...
  56. pmc Establishment of a robust single axis of cell polarity by coupling multiple positive feedback loops
    Tina Freisinger
    Max Planck Institute of Biochemistry, Cellular Dynamics and Cell Patterning, Am Klopferspitz 18, D 82152 Martinsried, Germany
    Nat Commun 4:1807. 2013
    ..Our results demonstrate how cells optimize symmetry breaking through coupling between multiple feedback loops...
  57. ncbi Identification of yeast Rho1p GTPase as a regulatory subunit of 1,3-beta-glucan synthase
    H Qadota
    Department of Biological Sciences, Graduate School of Science, University of Tokyo, Tokyo, Japan
    Science 272:279-81. 1996
    ..Glucan synthase from mutants expressing constitutively active Rho1p did not require exogenous guanosine triphosphate for activity...
  58. ncbi A function for the AP3 coat complex in synaptic vesicle formation from endosomes
    V Faundez
    Department of Biochemistry and Biophysics and Hormone Research Institute, University of California, San Francisco 94143 0534, USA
    Cell 93:423-32. 1998
    ..Budding from washed membranes can be reconstituted with purified AP3 and recombinant ARF1. We conclude that AP3 coating is involved in at least one pathway of small vesicle formation from endosomes...
  59. pmc Kissing G domains of MnmE monitored by X-ray crystallography and pulse electron paramagnetic resonance spectroscopy
    Simon Meyer
    Department of Structural Biology, Max Planck Institute of Molecular Physiology, Dortmund, Germany
    PLoS Biol 7:e1000212. 2009
    ..They show the nucleotide-dependent dynamic movements of the G domains around two swivel positions relative to the rest of the protein, and they are of crucial importance for understanding the mechanistic principles of this GAD...
  60. ncbi In vivo functional characterization of the Saccharomyces cerevisiae 60S biogenesis GTPase Nog1
    Jennifer L Fuentes
    Department of Molecular, Cellular and Developmental Biology, University of Michigan, 830 North University Avenue, Ann Arbor, MI 48109, USA
    Mol Genet Genomics 278:105-23. 2007
    ..Several conserved charge-to-alanine substitutions outside the GTPase domain display modest growth phenotypes indicating that these residues are not critical for function...
  61. pmc The mechanisms of microtubule catastrophe and rescue: implications from analysis of a dimer-scale computational model
    Gennady Margolin
    Department of Applied and Computational Mathematics and Statistics, University of Notre Dame, Notre Dame, IN 46556, USA
    Mol Biol Cell 23:642-56. 2012
    ....
  62. ncbi Kinetic analysis of interaction of eukaryotic release factor 3 with guanine nucleotides
    Vera P Pisareva
    Department of Microbiology and Immunology, State University of New York Downstate Medical Center, Brooklyn, New York 11203, USA
    J Biol Chem 281:40224-35. 2006
    ..Guanine nucleotide binding and exchange on eRF3, which therefore depends on stimulation by eRF1, is entirely different from that on prokaryotic RF3 and unusual among GTPases...
  63. pmc The structure of the RNA-dependent RNA polymerase from bovine viral diarrhea virus establishes the role of GTP in de novo initiation
    Kyung H Choi
    Department of Biological Sciences, Purdue University, 915 West State Street, West Lafayette, IN 47907, USA
    Proc Natl Acad Sci U S A 101:4425-30. 2004
    ..The putative binding sites of previously reported BVDV inhibitors are also discussed...
  64. pmc Novel mechanism of RNA repair by RtcB via sequential 2',3'-cyclic phosphodiesterase and 3'-Phosphate/5'-hydroxyl ligation reactions
    Naoko Tanaka
    Molecular Biology Program, Sloan Kettering Institute, New York, New York 10065, USA
    J Biol Chem 286:43134-43. 2011
    ..This adduct is sensitive to acid and hydroxylamine but resistant to alkali, consistent with a phosphoramidate bond...
  65. ncbi The nuclear transport factor karyopherin beta binds stoichiometrically to Ran-GTP and inhibits the Ran GTPase activating protein
    M Floer
    Laboratory of Cell Biology, Howard Hughes Medical Institute, Rockefeller University, New York, New York 10021, USA
    J Biol Chem 271:5313-6. 1996
    ..Ran-GDP bound only weakly to karyopherin beta. We propose that stoichiometric complex formation between karyopherin beta and Ran-GTP renders Ran-GTP inaccessible to RanGAP...
  66. ncbi Regulation of Tiam1 nucleotide exchange activity by pleckstrin domain binding ligands
    A M Crompton
    Onyx Pharmaceuticals, Richmond, California 94806, USA
    J Biol Chem 275:25751-9. 2000
    ..In addition, this paper introduces ascorbyl stearate as a pleckstrin domain ligand that can modulate the activity of certain pleckstrin domain-containing proteins...
  67. pmc Tet(M)-promoted release of tetracycline from ribosomes is GTP dependent
    V Burdett
    Department of Microbiology, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Bacteriol 178:3246-51. 1996
    ..Furthermore, while Tet(M) protects translation from tetracycline inhibition in a defined system, it is unable to substitute for either EF-G or elongation factor Tu...
  68. pmc Functional reconstitution of an atypical G protein heterotrimer and regulator of G protein signaling protein (RGS1) from Arabidopsis thaliana
    Janice C Jones
    Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    J Biol Chem 286:13143-50. 2011
    ..Thus, despite profound differences in the mechanisms of activation, the Arabidopsis G protein is readily inactivated by its cognate RGS protein and forms a stable, GDP-bound, heterotrimeric complex similar to that found in animals...
  69. pmc Preferential binding of a kinesin-1 motor to GTP-tubulin-rich microtubules underlies polarized vesicle transport
    Takao Nakata
    Department of Cell Biology and Anatomy, Graduate School of Medicine, University of Tokyo, 7 3 1 Hongo, Tokyo 113 0033, Japan
    J Cell Biol 194:245-55. 2011
    ..Collectively, these data suggest that the abundance of GTP-tubulin in axonal microtubules may underlie selective KIF5 localization and polarized axonal vesicular transport...
  70. pmc GTP binding to the ROC domain of DAP-kinase regulates its function through intramolecular signalling
    Rodrigo Carlessi
    Department of Molecular Genetics, Weizmann Institute of Science, Rehovot, Israel
    EMBO Rep 12:917-23. 2011
    ..This study presents a new mechanism of intramolecular signal transduction, by which GTP binding operates in cis to affect the catalytic activity of a distal domain in the protein...
  71. ncbi Uncoupling of initiation factor eIF5B/IF2 GTPase and translational activities by mutations that lower ribosome affinity
    Byung Sik Shin
    Laboratory of Gene Regulation and Development, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Cell 111:1015-25. 2002
    ..The translational defect suggests eIF5B stabilizes Met-tRNA(i)(Met) binding and that GTP hydrolysis by eIF5B is a checkpoint monitoring 80S ribosome assembly in the final step of translation initiation...
  72. pmc Filament structure, organization, and dynamics in MreB sheets
    David Popp
    ERATO Actin Filament Dynamics Project, Japan Science and Technology Corporation, 1 1 1 Kouto, Sayo, Hyogo 679 5148, Japan
    J Biol Chem 285:15858-65. 2010
    ..This suggests that the bacterial cell may exploit various nucleotides to generate different filament structures within cables for specific MreB-based functions...
  73. ncbi Structural evidence for feedback activation by Ras.GTP of the Ras-specific nucleotide exchange factor SOS
    S Mariana Margarit
    Department of Molecular Genetics and Microbiology, State University of New York at Stony Brook, Stony Brook, NY 11794, USA
    Cell 112:685-95. 2003
    ..These results demonstrate the existence of a positive feedback mechanism for the spatial and temporal regulation of Ras...
  74. ncbi The ribosomal stalk plays a key role in IF2-mediated association of the ribosomal subunits
    Chenhui Huang
    Department of Cell and Molecular Biology, Uppsala University, BMC, Box 596, S 75 124 Uppsala, Sweden
    J Mol Biol 399:145-53. 2010
    ..L12 depletion, however, did not affect the individual rates of the subsequent steps including GTP hydrolysis on IF2 and P(i) release. Thus, L12 is not a GTPase activating protein (GAP) for IF2 unlike as suggested for EF-G and EF-Tu...
  75. ncbi Structure of the 30S translation initiation complex
    Angelita Simonetti
    Institute of Genetics and of Molecular and Cellular Biology, Department of Structural Biology and Genomics, Illkirch F 67404, France
    Nature 455:416-20. 2008
    ....
  76. pmc An essential role for ARF6-regulated membrane traffic in adherens junction turnover and epithelial cell migration
    F Palacios
    Department of Biological Sciences and the Walther Cancer Institute, University of Notre Dame, Notre Dame, IN 46556-0369, USA
    EMBO J 20:4973-86. 2001
    ..Finally, we show that ARF6 exerts its role downstream of v-Src activation during the disassembly of AJs. These findings document an essential role for ARF6- regulated membrane traffic in AJ disassembly and epithelial cell migration...
  77. pmc Deletion of the ftsZ-like gene results in the production of superparamagnetic magnetite magnetosomes in Magnetospirillum gryphiswaldense
    Yao Ding
    State Key Laboratories for Agrobiotechnology and College of Biological Sciences, China Agricultural University, Beijing, China
    J Bacteriol 192:1097-105. 2010
    ..Our findings suggest that the FtsZ-like protein is required for synthesis of SD particles and magnetosomes in M. gryphiswaldense...
  78. ncbi Crystal structure of a mutant elongation factor G trapped with a GTP analogue
    Sebastian Hansson
    Department of Molecular Biophysics, Lund University, Box 124, S 211 00 Lund, Sweden
    FEBS Lett 579:4492-7. 2005
    ....
  79. pmc The Rap-RapGAP complex: GTP hydrolysis without catalytic glutamine and arginine residues
    Andrea Scrima
    Department of Structural Biology, Max Planck Institute of Molecular Physiology, Dortmund, Germany
    EMBO J 27:1145-53. 2008
    ..The structure and biochemical data furthermore show that the RapGAP homologous region of the tumour suppressor Tuberin is sufficient for catalysis on Rheb...
  80. ncbi Peptidyl-tRNA regulates the GTPase activity of translation factors
    Andrey V Zavialov
    Department of Cell and Molecular Biology, BMC, Uppsala University, Box 596, S 75124 Uppsala, Sweden
    Cell 114:113-22. 2003
    ..We also propose a model for translocation of tRNAs in two separate steps, which clarifies the roles of EF-G.GTP and GTP hydrolysis in this process...
  81. pmc Septin ring assembly involves cycles of GTP loading and hydrolysis by Cdc42p
    Amy S Gladfelter
    Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, NC 27710, USA
    J Cell Biol 156:315-26. 2002
    ....
  82. pmc Control of phosphate release from elongation factor G by ribosomal protein L7/12
    Andreas Savelsbergh
    Institute of Molecular Biology, University of Witten Herdecke, Witten, Germany
    EMBO J 24:4316-23. 2005
    ....
  83. pmc Dynamin GTPase domain mutants block endocytic vesicle formation at morphologically distinct stages
    H Damke
    Department of Cell Biology, The Scripps Research Institute, La Jolla, California 92037, USA
    Mol Biol Cell 12:2578-89. 2001
    ..Nonetheless, we find that overexpression of dynamin mutants with subtle differences in their GTPase properties can lead to the accumulation of distinct intermediates in endocytic coated vesicle formation...
  84. ncbi The determinants that govern microtubule assembly from the atomic structure of GTP-tubulin
    Agata Nawrotek
    Laboratoire d Enzymologie et Biochimie Structurales LEBS, Centre de Recherche de Gif, CNRS, Bat 34, 1, Avenue de la Terrasse, 91198 Gif sur Yvette, France
    J Mol Biol 412:35-42. 2011
    ..They also suggest a model for the way the tubulin structure is modified in relation to microtubule assembly...
  85. ncbi LRRK1 protein kinase activity is stimulated upon binding of GTP to its Roc domain
    Daniel Korr
    Research Laboratories, Schering AG, Muellerstr 178, 13342 Berlin, Germany
    Cell Signal 18:910-20. 2006
    ..Ultimately, the results presented for LRRK1 may contribute to the elucidation of LRRK2's role in the pathogenesis of Parkinson's disease...
  86. ncbi Phosphorylation of the Cdc42 exchange factor Cdc24 by the PAK-like kinase Cla4 may regulate polarized growth in yeast
    M P Gulli
    Swiss Institute for Experimental Cancer Research Chemin des Boveresses 155 1066 Epalinges, Vaud, Switzerland
    Mol Cell 6:1155-67. 2000
    ....
  87. pmc New insights into how the Rho guanine nucleotide dissociation inhibitor regulates the interaction of Cdc42 with membranes
    Jared L Johnson
    Department of Chemistry, Cornell University, Ithaca, New York 14853, USA
    J Biol Chem 284:23860-71. 2009
    ....
  88. pmc A bacterial Ras-like small GTP-binding protein and its cognate GAP establish a dynamic spatial polarity axis to control directed motility
    Yong Zhang
    Institut de Microbiologie de la Méditerranée Université Aix Marseille Laboratoire de Chimie Bactérienne, Marseille, France
    PLoS Biol 8:e1000430. 2010
    ..In summary, our results provide insight into how bacteria regulate their polarity dynamically, revealing unsuspected conserved regulations with eukaryots...
  89. pmc Deciphering the catalytic machinery in 30S ribosome assembly GTPase YqeH
    Baskaran Anand
    Department of Biological Sciences and Bioengineering, Indian Institute of Technology, Kanpur, Uttar Pradesh, India
    PLoS ONE 5:e9944. 2010
    ..Such GTPases were categorized as HAS-GTPases and were proposed to follow a catalytic mechanism, different from the Ras-like proteins...
  90. ncbi Dynamics and mechanics of the microtubule plus end
    Joe Howard
    Max Plank Institute of Molecular Cell Biology and Genetics MPI CBG, Pfotenhauerstrasse 108, 01307 Dresden, Germany
    Nature 422:753-8. 2003
    ..Each mode is associated with a specific end structure on which end-binding proteins can assemble to modulate dynamics and couple the dynamic properties of microtubules to the movement of cellular structures...
  91. pmc In vivo mutational analysis of YtvA from Bacillus subtilis: mechanism of light activation of the general stress response
    Marcela Avila-Perez
    Swammerdam Institute for Life Science, University of Amsterdam, 1018 WV Amsterdam, The Netherlands
    J Biol Chem 284:24958-64. 2009
    ..The data obtained are integrated into a model for the structure of full-length YtvA, which presumably functions as a dimer...
  92. ncbi Six GTP-binding proteins of the Era/Obg family are essential for cell growth in Bacillus subtilis
    Takuya Morimoto
    Department of Microbial Cell Biology, Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916 5, Takayama, Ikoma, Nara 630 0101, Japan
    Microbiology 148:3539-52. 2002
    ..Collectively, these results suggest that members of the GTP-binding Era/Obg family are important proteins with precise, yet still not fully understood, roles in bacterial growth and viability...
  93. pmc Ligand interactions with eukaryotic translation initiation factor 2: role of the gamma-subunit
    F L Erickson
    University of Texas at Dallas, Department of Molecular and Cell Biology, Richardson 75083 0688, USA
    EMBO J 15:6311-20. 1996
    ..These studies indicate that the gamma-subunit of eIF-2 does indeed provide EF-Tu-like function to the eIF-2 complex, and further suggest that the level of Met-tRNAi(Met) is critical for maintaining wild-type rates of initiation in vivo...
  94. ncbi Novel G proteins, Rag C and Rag D, interact with GTP-binding proteins, Rag A and Rag B
    T Sekiguchi
    Department of Molecular Biology, Graduate School of Medical Science, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka 812 8582, Japan
    J Biol Chem 276:7246-57. 2001
    ..Both Rag C and Rag D changed their subcellular localization, depending on the nucleotide-bound state of Rag A. In a similar way, the disruption of S. cerevisiae GTR1 resulted in a change in the localization of Gtr2p...
  95. pmc Elongation factor G stabilizes the hybrid-state conformation of the 70S ribosome
    P Clint Spiegel
    Center for Molecular Biology of RNA, Department of Molecular, Cell and Developmental Biology, University of California Santa Cruz 95064, USA
    RNA 13:1473-82. 2007
    ..Our results show that binding of EF-G in the presence of GDPNP or GDP.fusidic acid stabilizes the ribosomal intermediate hybrid state, but that complete translocation is supported only by EF-G.GTP or EF-G.GDPNP...
  96. ncbi GTPase activity of dynamin and resulting conformation change are essential for endocytosis
    B Marks
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK
    Nature 410:231-5. 2001
    ..Rather, efficient GTP hydrolysis and an associated conformational change are also required. These data argue that dynamin has a mechanochemical function in vesicle scission...
  97. ncbi A novel binding protein composed of homophilic tetramer exhibits unique properties for the small GTPase Rab5
    Kota Saito
    Department of Physiological Chemistry, Graduate School of Pharmaceutical Sciences, University of Tokyo, Tokyo 113 0033, Japan
    J Biol Chem 277:3412-8. 2002
    ..Possible roles of the quaternary structure have been discussed in terms of the Rab5-mediated signaling...
  98. pmc Nucleotide-dependent bending flexibility of tubulin regulates microtubule assembly
    Hong Wei Wang
    Howard Hughes Medical Institute, University of California Berkeley, Berkeley, California 94720 3200, USA
    Nature 435:911-5. 2005
    ..Closure into a microtubule does not require GTP hydrolysis...
  99. ncbi G-domain dimerization orchestrates the tRNA wobble modification reaction in the MnmE/GidA complex
    Simon Meyer
    Department of Structural Biology, Max Planck Institute of Molecular Physiology, Otto Hahn Str 11, 44227 Dortmund, Germany
    J Mol Biol 392:910-22. 2009
    ..We finally show that an active GTPase, an intact MnmE/GidA communication, and dimerization of G-domains are necessary for in vivo functioning since mutations disrupting either result in a respiratory deficient phenotype in yeast...
  100. pmc Conserved functions of membrane active GTPases in coated vesicle formation
    Thomas J Pucadyil
    Department of Cell Biology, The Scripps Research Institute TSRI, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Science 325:1217-20. 2009
    ..These GTPases, which associate with and dissociate from donor membranes in a guanosine triphosphate-dependent manner, can also actively remodel membranes...
  101. pmc The fibronectin-binding integrins alpha5beta1 and alphavbeta3 differentially modulate RhoA-GTP loading, organization of cell matrix adhesions, and fibronectin fibrillogenesis
    Erik H J Danen
    Division of Cell Biology, Netherlands Cancer Institute, 1066 CX Amsterdam, Netherlands
    J Cell Biol 159:1071-86. 2002
    ..Our findings demonstrate that the pattern of fibronectin receptors expressed on a cell dictates the ability of fibronectin to stimulate RhoA-mediated organization of cell matrix adhesions...

Research Grants64

  1. Rac GTPase signaling during embryonic salivary gland branching morphogenesis
    Sharon Sequeira; Fiscal Year: 2012
    ..basement membrane;ECM, extracellular matrix;GEF, guanine nucleotide exchange factor;GTPase, guanosine triphosphate hydroxylase;IB, immunoblotting;IF, immunofluorescence;IP, immunoprecipitation;Par, partitioning-defective ..
  2. Mechanism of G protein Activation by Ric-8A
    Stephen R Sprang; Fiscal Year: 2013
    ..a Guanine nucleotide Exchange Factor (GEF) that catalyzes the exchange of guanosine diphosphate (GDP) for guanosine triphosphate (GTP) at the active site of G?. This reaction transitions G? to the so-called "activated" state...
  3. NEUROFIBROMATOSIS TYPE 1 GENE REGULATES MYELOPOIESIS
    DAVID CLAPP; Fiscal Year: 2001
    ..Ras) family of protooncogenes control cellular growth and differentiation by cycling between an active guanosine triphosphate (GTP)-bound state and an inactive guanosine diphosphate (GDP)-bound state...
  4. Medical Management of Pediatric Neurotransmitter Disorders- A Multidisciplinary
    K Michael Gibson; Fiscal Year: 2007
    ..that will be discussed at this conference include: aromatic L- amino acid decarboxylase deficiency (AADC), guanosine triphosphate cyclohydrolase deficiency (Segawa's disease or DOPA-responsive dystonia, GTPCH), tyrosine hydroxylase ..
  5. ANTICOCCIDIAL DRUGS AND DRUG RESISTANCE IN TOXPLASMOSIS
    ELMER PFEFFERKORN; Fiscal Year: 1991
    ..mutants, of T gondii resistant to sulfadiazine will be examined by comparing the wild type and mutant guanosine triphosphate cyclophydrolases...
  6. Dopamine regulation in parkinsonian rat by gene therapy
    Un Kang; Fiscal Year: 2006
    ..We will use adeno-associated virus vectors to deliver tyrosine hydroxylase and guanosine triphosphate (GTP) cyclohydrolase 1 genes...
  7. Novel 5'-end tagging of mRNA for cDNA synthesis or RACE
    John Archdeacon; Fiscal Year: 2001
    ..tagging a synthetic oligonucleotide to the 5'-end of full-length mRNAs using the cap structure (7-methyl guanosine triphosphate, m7Gppp). There are two major technical limitations in full-length cDNA library construction...
  8. PEPTIDE PROBES OF THE 5HT1A RECEPTOR/G PROTEIN INTERFAC
    Keith Parker; Fiscal Year: 1999
    ..in changes of the second messenger cyclic AMP or the enzyme adenylyl cyclase, and by measurement of guanosine triphosphate (GTP)/ G protein binding...
  9. Zeiss LSM510 META confocal-fluorescence spectroscopy
    HAROLD ERICKSON; Fiscal Year: 2003
    ..Projects for this module are proposed by two faculty from Cell Biology and two from Biochemistry. This will be the only FCS facility at Duke University, and we expect additional projects from across the campus. ..
  10. G-protein signal coordination by RGS12
    DAVID SIDEROVSKI; Fiscal Year: 2009
    ..We wish to study one RGS protein, RGS12, that interferes with the actions of the neurotransmitter GABA in neurons that control pain processing. This work should lead to the discovery of new pain-controlling drugs. ..
  11. Post-transcriptional regulation of TNF-alpha production
    Paul Anderson; Fiscal Year: 2005
    ..These studies might also identify molecular targets for the development of orally available TNFa blockers that can be used to treat patients with rheumatoid arthritis. ..
  12. BIOCHEMICAL STRATEGIES TO INCREASE LEUKEMIA RESPONSE
    Varsha Gandhi; Fiscal Year: 2005
    ..We feel that knowledge gained through these aims will assist us in designing optimal therapy of leukemia with nucleoside analogs. ..
  13. Ped Endocrine Career Development in Diabetes Research
    Charles Stanley; Fiscal Year: 2007
    ..The Program is strongly supported by access to a superb range of institutional resources, including the CHOP GCRC and the UPenn DERC. Request is made for 2 Appointee slots in this Program each year. ..
  14. The role of FAST in immune-mediated inflammatory disease
    Paul Anderson; Fiscal Year: 2008
    ..Finally, we will generate transgenic mice that overexpress FAST and mutant mice that lack FAST to determine its importance in the initiation and/or propagation of immune-mediated inflammatory disease. ..
  15. International Medical Conference of Congenital Hyperinsulinism
    Charles Stanley; Fiscal Year: 2006
    ..unreadable] [unreadable] [unreadable]..
  16. SNARE PROTEINS IN PLATELET ALPHA-GRANULE SECRETION
    Robert Flaumenhaft; Fiscal Year: 2007
    ..abstract_text> ..
  17. ISLET DYSREGULATION IN INFANTS
    Charles Stanley; Fiscal Year: 2008
    ..The results of this research will provide essential information for improving the diagnosis and treatment of HI children and for understanding the basis of insulin regulation in normal humans. ..
  18. STRUCTURE AND FUNCTION OF SIGNAL TRANSDUCING COMPONENTS
    SRINIVAS RAVI IYENGAR; Fiscal Year: 2008
    ..abstract_text> ..
  19. Structural Study of ARF-GTPase Regulators and Effectors
    Jonathan Goldberg; Fiscal Year: 2007
    ..8 Angstroms resolution in-house, which we are working to improve. These studies will provide insight to the processes of GTP-dependent assembly of vesicular coat complexes and coat-controlled GTP hydrolysis triggering disassembly. ..
  20. Chemical Genetic Analysis of Platelet Granule Secretion
    Robert Flaumenhaft; Fiscal Year: 2008
    ..Once identified, compounds will be tested in a previously established battery of platelet function tests and evaluated for antithrombotic potential in an in vivo murine model of thrombus formation. [unreadable] [unreadable] [unreadable]..
  21. Mechanistic studies of a novel G-alpha nucleotide cycle
    DAVID SIDEROVSKI; Fiscal Year: 2009
    ....
  22. Phase I study of 8-Cl-adenosine in CLL (IND 68,229)
    Varsha Gandhi; Fiscal Year: 2010
    ..The investigators state that these Aims will establish utility of 8-Cl-Ado in CLL, provide a dose for Phase 2 study, and knowledge for optimal use of this agent alone and in combination. ..
  23. Characterization of mammalian stress granules
    Paul J Anderson; Fiscal Year: 2010
    ..These studies have implications for our understanding of stress-regulated pathological conditions, including Alzheimer's disease, diabetes and cancer. ..
  24. Protein Prenyltransferases in Glucose-Stimulated Insulin Secretion
    Anjaneyulu Kowluru; Fiscal Year: 2010
    ....
  25. SIGNALING PATHWAYS INTERACTIONS AND MAMMARY TUMOR
    SRINIVAS RAVI IYENGAR; Fiscal Year: 2004
    ..abstract_text> ..
  26. Real-Time Fluorescence Assay:RGS Domain GAP Activit(RMI)
    DAVID SIDEROVSKI; Fiscal Year: 2008
    ..unreadable]PERFORMANCE SITE ========================================Section End=========================================== ..
  27. ROLE OF PROTEIN PHOSPHATASES IN INSULIN SECRETION
    Anjaneyulu Kowluru; Fiscal Year: 2004
    ....
  28. Role of Prostaglandins & Phospholipase A in melanocytes
    Glynis Scott; Fiscal Year: 2009
    ..We hypothesize that PG and phospholipases stimulate HM dendricity, MS transfer &pigmentation through different mechanisms that act synergistically to modulate cutaneous pigmentation following UVR. ..
  29. MOLECULAR BASIS OF A NEW FORM OF HYPERINSULINISM
    CHARLES ALFRED STANLEY; Fiscal Year: 2010
    ....
  30. ROLE OF PSGL 1 IN NATURAL KILLER CELL FUNCTION
    Paul Anderson; Fiscal Year: 2003
    ..Consequently, the overall goal of the proposed experiments is to determine how PEN5:L-selectin interactions influence NK cell function. ..
  31. Role of guanine nucleotides in ischemic renal injury
    Pierre Dagher; Fiscal Year: 2006
    ..These studies will establish GTP as a key . modulator of ischemic injury and might lead to new therapeutic options for this devastating disease. ..
  32. Biophsyics of Receptor/G-protein Interactions
    OLEG KISSELEV; Fiscal Year: 2007
    ..abstract_text> ..
  33. EICOSANOIDS, VASCULAR TONE AND BLOOD PRESSURE
    WILLIAM BRYSON CAMPBELL; Fiscal Year: 2010
    ..These studies will test the hypothesis that endogenous LO metabolites are important regulators of vascular tone and blood pressure in mice. ..
  34. Modeling Cell Receptor Signaling Pathways
    Haluk Resat; Fiscal Year: 2008
    ..Based on such knowledge, therapeutic treatment regimen can be personalized according to a patient[unreadable]s HER receptor expression profile. ..
  35. Predicting common protein mechanisms by the light of evolution
    ANDREW NEUWALD; Fiscal Year: 2009
    ..And (v) propose molecular mechanisms based on these analyses. ..
  36. PROTEINS INTERACTING WITH DISHEVELLED IN VERTEBRATES
    BENJAMIN CHEYETTE; Fiscal Year: 2003
    ..The developmental expression of Dishevelled-interactors will be characterized in human brain tissue, through collaboration with colleagues within the Department of Psychiatry at the University of Washington. ..
  37. Mechanisms of Rab Protein Activation and Function
    Ruth Collins; Fiscal Year: 2009
    ....
  38. Gefitinib-sensitive EGF receptor mutants in lung cancer
    Jeffrey Settleman; Fiscal Year: 2009
    ....
  39. Mechanisms of glutamate dehydrogenase allostery
    Thomas Smith; Fiscal Year: 2008
    ..abstract_text> ..
  40. Mechanism of RIN1 Signaling in Neuronal Plasticity
    John Colicelli; Fiscal Year: 2007
    ..Special focus will be placed on dendritic spine remodeling because the connection of this phenomenon to learning remains conjectural. [unreadable] [unreadable]..
  41. MECHANISMS OF CHLORPYRIFOS DEVELOPMENTAL NEUROTOXICITY
    Theodore Slotkin; Fiscal Year: 2004
    ..Molecular mechanisms of developmental neurotoxicity can be determined and linked to eventual alterations in behavioral performance. ..
  42. Cloning and Engineering of Nicking enzymes
    Shuang yong Xu; Fiscal Year: 2004
    ..abstract_text> ..
  43. LC-MS/MS for Biomedical Research
    William Campbell; Fiscal Year: 2004
    ..About 75-80% of instrument time will be available to these participants and the remaining time will be available to other investigators at the College. ..
  44. TUBULIN STRUCTURE BY ELECTRON CRYSTALLOGRAPHY
    KENNETH DOWNING; Fiscal Year: 2003
    ..abstract_text> ..
  45. RHO GTPASE SIGNALING IN EMBRYO MORPHOGENESIS
    Jeffrey Settleman; Fiscal Year: 2003
    ..abstract_text> ..
  46. DRUGS AND DEVELOPMENT OF THE ADRENERGIC NERVOUS SYSTEM
    Theodore Slotkin; Fiscal Year: 2003
    ....
  47. Iron transport and its regulation in C. elegans
    Marianne Wessling Resnick; Fiscal Year: 2002
    ..elegans; 3) Use genetic selection to establish transport mutants useful for the elucidation of additional proteins involved in iron import and/or export and the factors that regulate these processes. ..
  48. INTERACTIONS BETWEEN F-ACTIN, MYOSIN II AND MICROTUBULES
    Garrett Odell; Fiscal Year: 2001
    ..elegans blastomeres. ..
  49. ACTIN DYNAMICS AND CELL LOCOMOTION
    Timothy Mitchison; Fiscal Year: 2004
    ..For perturbation experiments we will use protein tools, and also cell permeable chemicals coming out of a screening effort at the Harvard Institute of Chemistry and Cell Biology (ICCB). ..
  50. Indentification of AKT2/aPKC Substrates in Adipocytes
    Prem Sharma; Fiscal Year: 2005
    ..We believe that the findings from the proposed investigations will improve our general understanding of players of insulin resistance and offer potential insight into new therapeutic modalities. ..
  51. Saliva Analysis with an Array Sensor
    Eric Anslyn; Fiscal Year: 2005
    ....
  52. Pathogenesis of HIVAN: the role of Nef
    JOHN HE; Fiscal Year: 2007
    ..These studies should elucidate essential mechanism responsible for the development of HIV-associated nephropathy and how Nef plays a role in this pathologic process. ..
  53. Tissue Transglutaminase in Neurologic Injury
    THUNG SHEN LAI; Fiscal Year: 2007
    ..The inhibitors will be used to investigate the role of TTG in the pathogenesis of expanded polyQ diseases. Current proposal will lead to new therapy for HD and other neurologic disorders. [unreadable] [unreadable] [unreadable]..
  54. Lethal Mutagenesis as an Antiviral Strategy
    Craig Cameron; Fiscal Year: 2007
    ..Lethal mutagens should have broad-spectrum activity against RNA viruses, thus better equipping this nation to deal with the potential use of RNA viruses as biological weapons. [unreadable] [unreadable]..
  55. West Nile Virus Life Cycle in Flavivirus-Resistant Cells
    Pei Yong Shi; Fiscal Year: 2006
    ..abstract_text> ..
  56. MECHANISMS OF MICROTUBULE AND MITOTIC SPINDLE ASSEMBLY
    Lynne Cassimeris; Fiscal Year: 2006
    ..To address these issues, we will apply a number of fluorescence-based imaging methods combined with siRNA or injection of function-blocking proteins or antibodies. ..
  57. A Genetic System Able to Screen for Active DNA Demethylation
    Shuang yong Xu; Fiscal Year: 2006
    ..In addition, knowledge of the mechanism of demethylation could be crucial in converting fully differentiated adult stem cells into totipotent stem cells. [unreadable] [unreadable] [unreadable]..
  58. Novel Pathways of Polyphosphate Synthesis
    Arthur Kornberg; Fiscal Year: 2005
    ..abstract_text> ..
  59. Pathobiology of HMG-CoA reductase inhibitors in diabetes
    Yashpal Kanwar; Fiscal Year: 2006
    ....
  60. INITIATION OF HEPATITIS C VIRUS TRANSLATION
    CHRISTOPHER HELLEN; Fiscal Year: 2001
    ..abstract_text> ..
  61. Modulation of Oxidative Damage by Tea Polyphenols
    Jia Sheng Wang; Fiscal Year: 2002
    ..The results of this proposed study will help to understand the mechanisms of antioxidative role of tea polyphenols in modulating human hepatocarcinogenesis caused by hepatitis B/C viruses and aflatoxins. ..
  62. RECOGNITION AND SORTING OF GLYCOPROTEINS
    Michael Roth; Fiscal Year: 2002
    ....