adenosine monophosphate

Summary

Summary: Adenylic acid. Adenine nucleotide containing one phosphate group esterified to the sugar moiety in the 2'-, 3'-, or 5'-position.

Top Publications

  1. ncbi Ca2+/calmodulin-dependent protein kinase kinase-beta acts upstream of AMP-activated protein kinase in mammalian cells
    Angela Woods
    Cellular Stress Group, MRC Clinical Sciences Centre, Imperial College, Hammersmith Hospital, Du Cane Road, London W12 0NN, United Kingdom
    Cell Metab 2:21-33. 2005
  2. ncbi The AMP-activated protein kinase pathway--new players upstream and downstream
    D Grahame Hardie
    Division of Molecular Physiology, Wellcome Trust Biocentre, University of Dundee, Dow Street, Dundee, DD1 5EH, Scotland, UK
    J Cell Sci 117:5479-87. 2004
  3. pmc Investigating the mechanism for AMP activation of the AMP-activated protein kinase cascade
    Matthew J Sanders
    Cellular Stress Group, MRC Clinical Sciences Centre, Imperial College, Hammersmith Hospital, Du Cane Road, London W12 0NN, UK
    Biochem J 403:139-48. 2007
  4. pmc Mechanism of action of A-769662, a valuable tool for activation of AMP-activated protein kinase
    Olga Göransson
    Division for Diabetes, Metabolism and Endocrinology, Department of Experimental Medical Science, Lund University, Lund, Sweden
    J Biol Chem 282:32549-60. 2007
  5. pmc Specificity prediction of adenylation domains in nonribosomal peptide synthetases (NRPS) using transductive support vector machines (TSVMs)
    Christian Rausch
    Center for Bioinformatics Tübingen ZBIT, University of Tubingen, Germany
    Nucleic Acids Res 33:5799-808. 2005
  6. ncbi Structural basis for AMP binding to mammalian AMP-activated protein kinase
    Bing Xiao
    MRC National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, UK
    Nature 449:496-500. 2007
  7. ncbi T regulatory and primed uncommitted CD4 T cells express CD73, which suppresses effector CD4 T cells by converting 5'-adenosine monophosphate to adenosine
    James J Kobie
    David H Smith Center for Vaccine Biology and Immunology and Department of Microbiology and Immunology, University of Rochester Medical Center, Rochester, NY 14642, USA
    J Immunol 177:6780-6. 2006
  8. pmc De-AMPylation of the small GTPase Rab1 by the pathogen Legionella pneumophila
    M Ramona Neunuebel
    Cell Biology and Metabolism Program, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Science 333:453-6. 2011
  9. pmc The regulation of AMP-activated protein kinase by phosphorylation
    S C Stein
    Cellular Stress Group, MRC Clinical Sciences Centre, Imperial College School of Medicine, Hammersmith Hospital, DuCane Road, London W12 0NN, U K
    Biochem J 345:437-43. 2000
  10. ncbi The neurodegenerative disease protein aprataxin resolves abortive DNA ligation intermediates
    Ivan Ahel
    Cancer Research UK, London Research Institute, Clare Hall Laboratories, South Mimms, Herts EN6 3LD, UK
    Nature 443:713-6. 2006

Research Grants

  1. Role of Chromogranin A in Metabolic Syndrome
    Sushil K Mahata; Fiscal Year: 2013
  2. Med1 in Liver Metabolism, Regeneration and Cancer
    Janardan K Reddy; Fiscal Year: 2013
  3. Regulation of microRNA-375 by cyclic-AMP in pancreatic beta-cells
    DAVID MATTHEW KELLER; Fiscal Year: 2010
  4. Respiration in Sepsis
    Claude A Piantadosi; Fiscal Year: 2013
  5. Fic-mediated Adenylylation
    CAROLYN ANN WORBY; Fiscal Year: 2013
  6. Impact of AMP-activated kinase on sex differences in hypertrophic cardiomyopathy
    John P Konhilas; Fiscal Year: 2013
  7. Pulmonary Vascular-Right Ventricular Axis Research Program
    Wai Hong Wilson Tang; Fiscal Year: 2013
  8. Development of inhibitors of AMP Deaminase Isoform 2 as a Mechanism for Treating
    RICHARD JOSEPH JOHNSON; Fiscal Year: 2010
  9. Sympathetic Regulation of AMPK in the control of non-shivering thermogenesis
    Jacob D Mulligan; Fiscal Year: 2012
  10. The Role of Leptin in Liver Fibrogenesis
    Frank A Anania; Fiscal Year: 2012

Detail Information

Publications271 found, 100 shown here

  1. ncbi Ca2+/calmodulin-dependent protein kinase kinase-beta acts upstream of AMP-activated protein kinase in mammalian cells
    Angela Woods
    Cellular Stress Group, MRC Clinical Sciences Centre, Imperial College, Hammersmith Hospital, Du Cane Road, London W12 0NN, United Kingdom
    Cell Metab 2:21-33. 2005
    ..These results demonstrate that AMPK is regulated by at least two upstream kinases and suggest that AMPK may play a role in Ca(2+)-mediated signal transduction pathways...
  2. ncbi The AMP-activated protein kinase pathway--new players upstream and downstream
    D Grahame Hardie
    Division of Molecular Physiology, Wellcome Trust Biocentre, University of Dundee, Dow Street, Dundee, DD1 5EH, Scotland, UK
    J Cell Sci 117:5479-87. 2004
    ....
  3. pmc Investigating the mechanism for AMP activation of the AMP-activated protein kinase cascade
    Matthew J Sanders
    Cellular Stress Group, MRC Clinical Sciences Centre, Imperial College, Hammersmith Hospital, Du Cane Road, London W12 0NN, UK
    Biochem J 403:139-48. 2007
    ..This model accounts for activation of AMPK by two distinct signals: a Ca2+-dependent pathway, mediated by CaMKKbeta and an AMP-dependent pathway, mediated by LKB1...
  4. pmc Mechanism of action of A-769662, a valuable tool for activation of AMP-activated protein kinase
    Olga Göransson
    Division for Diabetes, Metabolism and Endocrinology, Department of Experimental Medical Science, Lund University, Lund, Sweden
    J Biol Chem 282:32549-60. 2007
    ..We propose that this direct and specific AMPK activator will be a valuable experimental tool to understand the physiological roles of AMPK...
  5. pmc Specificity prediction of adenylation domains in nonribosomal peptide synthetases (NRPS) using transductive support vector machines (TSVMs)
    Christian Rausch
    Center for Bioinformatics Tübingen ZBIT, University of Tubingen, Germany
    Nucleic Acids Res 33:5799-808. 2005
    ..None of the predictive methods could infer any specificity for 2.4% of the sequences, suggesting completely new types of specificity...
  6. ncbi Structural basis for AMP binding to mammalian AMP-activated protein kinase
    Bing Xiao
    MRC National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, UK
    Nature 449:496-500. 2007
    ..The structure also suggests a mechanism for propagating AMP/ATP signalling whereby a phosphorylated residue from the alpha and/or beta subunits binds to the gamma subunit in the presence of AMP but not when ATP is bound...
  7. ncbi T regulatory and primed uncommitted CD4 T cells express CD73, which suppresses effector CD4 T cells by converting 5'-adenosine monophosphate to adenosine
    James J Kobie
    David H Smith Center for Vaccine Biology and Immunology and Department of Microbiology and Immunology, University of Rochester Medical Center, Rochester, NY 14642, USA
    J Immunol 177:6780-6. 2006
    ..Infiltration of either Treg or Thpp cells at inflammatory sites could potentially convert 5'-AMP generated by neutrophils or dying cells into the anti-inflammatory mediator adenosine, thus dampening excessive immune reactions...
  8. pmc De-AMPylation of the small GTPase Rab1 by the pathogen Legionella pneumophila
    M Ramona Neunuebel
    Cell Biology and Metabolism Program, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Science 333:453-6. 2011
    ..Legionella-containing vacuole (LCV), where it activates Rab1 and then AMPylates it by covalently adding adenosine monophosphate (AMP). L. pneumophila GTPase-activating protein LepB inactivates Rab1 before its removal from LCVs...
  9. pmc The regulation of AMP-activated protein kinase by phosphorylation
    S C Stein
    Cellular Stress Group, MRC Clinical Sciences Centre, Imperial College School of Medicine, Hammersmith Hospital, DuCane Road, London W12 0NN, U K
    Biochem J 345:437-43. 2000
    ..Furthermore, we provide evidence that phosphorylation of Thr(172) may be involved in the sensitivity of the AMPK complex to AMP...
  10. ncbi The neurodegenerative disease protein aprataxin resolves abortive DNA ligation intermediates
    Ivan Ahel
    Cancer Research UK, London Research Institute, Clare Hall Laboratories, South Mimms, Herts EN6 3LD, UK
    Nature 443:713-6. 2006
    ..These data indicate that neurological disorders associated with APTX mutations may be caused by the gradual accumulation of unrepaired DNA strand breaks resulting from abortive DNA ligation events...
  11. ncbi Management of cellular energy by the AMP-activated protein kinase system
    D Grahame Hardie
    Division of Molecular Physiology, Wellcome Trust Biocentre, University of Dundee, Dow Street, Dundee DD1 5EH, UK
    FEBS Lett 546:113-20. 2003
    ..A survey of the range of downstream targets for this important signalling pathway is presented...
  12. ncbi Structural insight into the autoinhibition mechanism of AMP-activated protein kinase
    Lei Chen
    MOE Key Laboratory of Bioinformatics, Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China
    Nature 459:1146-9. 2009
    ..The structural and biochemical data have shown the primary mechanism of AMPK autoinhibition and suggest a conformational switch model for AMPK activation by AMP...
  13. ncbi Thiazolidinediones can rapidly activate AMP-activated protein kinase in mammalian tissues
    Nathan K Lebrasseur
    Diabetes and Metabolism Research Unit, Boston University School of Medicine, 650 Albany St, X 820, Boston, MA 02118, USA
    Am J Physiol Endocrinol Metab 291:E175-81. 2006
    ..They suggest that this effect is associated with a change in cellular energy state and that it is not dependent on PPARgamma-mediated gene transcription...
  14. ncbi Genetic identification of microcystin ecotypes in toxic cyanobacteria of the genus Planktothrix
    Rainer Kurmayer
    Austrian Academy of Sciences, Institute for Limnology, Mondseestrasse 9, A 5310 Mondsee, Austria
    Microbiology 151:1525-33. 2005
    ....
  15. ncbi Structure of the complex between adenylate kinase from Escherichia coli and the inhibitor Ap5A refined at 1.9 A resolution. A model for a catalytic transition state
    C W Muller
    Institut für Organische Chemie und Biochemie der Universität, Freiburg, Germany
    J Mol Biol 224:159-77. 1992
    ..The mutants were classified. The concomitant increase of the Michaelis constants for ATP and AMP in the group of mutants that modify only the ATP-binding site cannot be explained...
  16. pmc β-Subunit myristoylation is the gatekeeper for initiating metabolic stress sensing by AMP-activated protein kinase (AMPK)
    Jonathan S Oakhill
    Protein Chemistry and Metabolism, Saint Vincent s Institute of Medical Research, University of Melbourne, 41 Victoria Parade, Fitzroy 3065, Australia
    Proc Natl Acad Sci U S A 107:19237-41. 2010
    ..Our results show that AMP regulates AMPK activation at the initial phosphorylation step, and that β-subunit myristoylation is important for transducing the metabolic stress signal...
  17. ncbi Crystal structure of ADP/AMP complex of Escherichia coli adenylate kinase
    Michael B Berry
    W M Keck Center for Computational Biology, Department of Biochemistry and Cell Biology, Rice University, Houston, Texas, USA
    Proteins 62:555-6. 2006
  18. ncbi Disruption of type 5 adenylyl cyclase enhances desensitization of cyclic adenosine monophosphate signal and increases Akt signal with chronic catecholamine stress
    Satoshi Okumura
    Cardiovascular Research Institute, Yokohama City University Graduate School of Medicine, Yokohama, Japan
    Circulation 116:1776-83. 2007
    Desensitization of the cyclic adenosine monophosphate signal protects cardiac myocytes against catecholamine stress, thus preventing the development of apoptosis...
  19. ncbi The closed conformation of a highly flexible protein: the structure of E. coli adenylate kinase with bound AMP and AMPPNP
    M B Berry
    W M Keck Center for Computational Biology, Rice University, Houston, Texas 77251 1892
    Proteins 19:183-98. 1994
    ..Finally, a comparison is made between the present structure and the structure of the heavy chain of muscle myosin...
  20. ncbi Targeted disruption of cd73/ecto-5'-nucleotidase alters thromboregulation and augments vascular inflammatory response
    Patrycja Koszalka
    Department of Cardiovascular Physiology, Heinrich Heine University Duesseldorf, Duesseldorf, Germany
    Circ Res 95:814-21. 2004
    ....
  21. ncbi Induction of torpor: mimicking natural metabolic suppression for biomedical applications
    Hjalmar R Bouma
    Department of Clinical Pharmacology, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands
    J Cell Physiol 227:1285-90. 2012
    ..Elucidating the underlying mechanisms of natural and pharmacologically induced torpor will speed the development of new clinical approaches to treat a variety of trauma and stress states in humans...
  22. ncbi The role of ecto-5'-nucleotidase/CD73 in glioma cell line proliferation
    Luci Bavaresco
    Departamento de Bioquimica, Instituto de Ciencias Basicas da Saude, Universidade Federal do Rio Grande do Sul, Rua Ramiro Barcelos, Porto Alegre, RS, Brazil
    Mol Cell Biochem 319:61-8. 2008
    ....
  23. ncbi AMPylation of Rho GTPases by Vibrio VopS disrupts effector binding and downstream signaling
    Melanie L Yarbrough
    Department of Molecular Biology, University of Texas UT Southwestern Medical Center, Dallas, TX 75390, USA
    Science 323:269-72. 2009
    ..Eukaryotic proteins were also directly modified with AMP, potentially expanding the repertoire of posttranslational modifications for molecular signaling...
  24. pmc CBS domains form energy-sensing modules whose binding of adenosine ligands is disrupted by disease mutations
    John W Scott
    Division of Molecular Physiology, Faculty of Life Sciences, Wellcome Trust Biocentre, University of Dundee, Scotland, United Kingdom
    J Clin Invest 113:274-84. 2004
    ..This shows that tandem pairs of CBS domains act, in most cases, as sensors of cellular energy status and, as such, represent a newly identified class of binding domain for adenosine derivatives...
  25. ncbi tRNA-dependent aminoacyl-adenylate hydrolysis by a nonediting class I aminoacyl-tRNA synthetase
    Ita Gruic-Sovulj
    Department of Chemistry and Biochemistry and Interdepartmental Program in Biomolecular Science and Engineering, University of California, Santa Barbara, California 93106 9510, USA
    J Biol Chem 280:23978-86. 2005
    ..Because glutaminyl-tRNA synthetase does not possess a spatially separate editing domain, these data demonstrate that a pre-transfer editing-like reaction can occur within the synthetic site of a class I tRNA synthetase...
  26. ncbi Clinically relevant concentrations of beta2-adrenergic agonists stimulate maximal cyclic adenosine monophosphate-dependent airspace fluid clearance and decrease pulmonary edema in experimental acid-induced lung injury
    Daniel F McAuley
    Cardiovascular Research Institute, University of California, San Francisco, CA, USA
    Crit Care Med 32:1470-6. 2004
    ....
  27. ncbi Anti-lipolytic action of AMP-activated protein kinase in rodent adipocytes
    Marie Daval
    INSERM U671, Centre de Recherches Biomedicales des Cordeliers, Universite Pierre et Marie Curie, 75270 Paris Cedex 06, France
    J Biol Chem 280:25250-7. 2005
    ..We conclude that, in mature adipocytes, AMP-activated protein kinase activation has a clear anti-lipolytic effect...
  28. pmc A mutation in CFTR modifies the effects of the adenylate kinase inhibitor Ap5A on channel gating
    Qian Dong
    Departments of Internal Medicine and Molecular Physiology and Biophysics, Howard Hughes Medical Institute, Roy J and Lucille A Carver College of Medicine, University of Iowa, Iowa City, Iowa 52242, USA
    Biophys J 95:5178-85. 2008
    ..These results also suggest that a better understanding of the adenylate kinase activity of CFTR may be of value in developing new therapeutic strategies for cystic fibrosis...
  29. ncbi Corticosteroid-induced improvement in the PC20 of adenosine monophosphate is more closely associated with reduction in airway inflammation than improvement in the PC20 of methacholine
    M van den Berge
    Department of Pulmonology, University Hospital Groningen, Groningen, The Netherlands
    Am J Respir Crit Care Med 164:1127-32. 2001
    ..We conclude that PC20 AMP is more sensitive to changes in acute airway inflammation than is PC20 methacholine, further reinforcing the notion that PC20 AMP can be a useful tool for monitoring the effects of antiinflammatory therapy...
  30. ncbi R-state AMP complex reveals initial steps of the quaternary transition of fructose-1,6-bisphosphatase
    Cristina V Iancu
    Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, Ames, 50011, USA
    J Biol Chem 280:19737-45. 2005
    ..The same hydrogen bonds reform but only after the quaternary transition to the T-state. Proposed here is a model that accounts for the quaternary transition and cooperativity in the inhibition of catalysis by AMP...
  31. pmc A role of erythrocytes in adenosine monophosphate initiation of hypometabolism in mammals
    Isadora Susan Daniels
    Department of Biochemistry and Molecular Biology, University of Texas Health Science Center, Houston, TX 77030, USA
    J Biol Chem 285:20716-23. 2010
    ..In summary, our investigations reveal that 5'-AMP mediated hypometabolism is probably triggered by reduced oxygen transport by erythrocytes initiated by uptake of 5'-AMP...
  32. pmc Small molecule antagonizes autoinhibition and activates AMP-activated protein kinase in cells
    Tao Pang
    National Center for Drug Screening, Shanghai Institute of Materia Medica, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences and Roche R and D Center China Ltd, Shanghai 201203, China
    J Biol Chem 283:16051-60. 2008
    ..This compound highlights the effort to discover novel AMPK activators and can be a useful tool for elucidating the mechanism responsible for conformational change and autoinhibitory regulation of AMPK...
  33. ncbi Mammalian AMP-activated protein kinase: functional, heterotrimeric complexes by co-expression of subunits in Escherichia coli
    Dietbert Neumann
    Institute of Cell Biology, Swiss Federal Institute of Technology, ETH Honggerberg, CH 8093 Zurich, Switzerland
    Protein Expr Purif 30:230-7. 2003
    ....
  34. ncbi Different sensitivities of mutants and chimeric forms of human muscle and liver fructose-1,6-bisphosphatases towards AMP
    Dariusz Rakus
    Department of Animal Physiology, Zoological Institute, Wroclaw University, Cybulskiego 30, 50 205 Wroclaw, Poland
    Biol Chem 384:51-8. 2003
    ....
  35. pmc PAP and NT5E inhibit nociceptive neurotransmission by rapidly hydrolyzing nucleotides to adenosine
    Sarah E Street
    Department of Cell and Molecular Physiology, University of North Carolina, CB 7545, Chapel Hill, North Carolina 27599, USA
    Mol Pain 7:80. 2011
    ....
  36. ncbi Bronchial responsiveness to methacholine and adenosine 5'-monophosphate in young children with asthma: their relationship with blood eosinophils and serum eosinophil cationic protein
    S H Choi
    Department of Pediatrics, Seoul National University Hospital, Seoul, Korea
    Allergy 62:1119-24. 2007
    ..The aim of this study was to investigate whether bronchial responsiveness to adenosine 5'-monophosphate (AMP) is more closely associated with blood eosinophil markers, compared with that to methacholine, in young children with asthma...
  37. ncbi Use of acetone to attain highly active and soluble DNA packaging protein Gp16 of Phi29 for ATPase assay
    Lisa P Huang
    Department of Pathobiology, Purdue University, West Lafayette, IN 47907, USA
    Virology 312:449-57. 2003
    ..However, mutation on amino acids G(248) and D(256) did not affect the function of gp16 in DNA packaging...
  38. ncbi Associative mechanism for phosphoryl transfer: a molecular dynamics simulation of Escherichia coli adenylate kinase complexed with its substrates
    Harini Krishnamurthy
    Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing 48224 1322, USA
    Proteins 58:88-100. 2005
    The ternary complex of Escherichia coli adenylate kinase (ECAK) with its substrates adenosine monophosphate (AMP) and Mg-ATP, which catalyzes the reversible transfer of a phosphoryl group between adenosine triphosphate (ATP) and AMP, was ..
  39. ncbi Lysyl-tRNA synthetase-generated lysyl-adenylate is a substrate for histidine triad nucleotide binding proteins
    Tsui Fen Chou
    Departments of Medicinal Chemistry and Chemistry, University of Minnesota, Minneapolis, Minnesota 55455, USA
    J Biol Chem 282:4719-27. 2007
    ..These results demonstrate that the lysyl-AMP intermediate formed by LysRS is a natural substrate for Hints and suggests a potential highly conserved regulatory role for Hints on LysRS and possibly other aminoacyl-tRNA synthetases...
  40. ncbi Changes in airway hyperresponsiveness following smoking cessation: comparisons between Mch and AMP
    Giovita Piccillo
    Smoking Cessation Research Centre, Department of Internal and Specialistic Medicine, University of Catania, Ascoli Tomaselli Hospitals, 95125 Catania, Italy
    Respir Med 102:256-65. 2008
    ....
  41. pmc Pain-relieving prospects for adenosine receptors and ectonucleotidases
    Mark J Zylka
    Department of Cell and Molecular Physiology, UNC Neuroscience Center, University of North Carolina, Chapel Hill, NC 27599, USA
    Trends Mol Med 17:188-96. 2011
    ..Collectively, these studies suggest the possibility of treating chronic pain in humans by targeting specific adenosine receptor subtypes in anatomically defined regions with agonists or with ectonucleotidases that generate adenosine...
  42. pmc The tyrocidine biosynthesis operon of Bacillus brevis: complete nucleotide sequence and biochemical characterization of functional internal adenylation domains
    H D Mootz
    Fachbereich Chemie Biochemie, Philipps Universitat Marburg, Germany
    J Bacteriol 179:6843-50. 1997
    ....
  43. ncbi Dissecting the role of 5'-AMP for allosteric stimulation, activation, and deactivation of AMP-activated protein kinase
    Marianne Suter
    Institute of Cell Biology, Schafmattstrasse 18, ETH Zurich, 8093 Zurich, Switzerland
    J Biol Chem 281:32207-16. 2006
    ..These data shed new light on physiologically important aspects of AMPK regulation...
  44. ncbi Altered extracellular ATP, ADP and AMP catabolism in glioma cell lines
    Marcia R Wink
    Departamento de Bioquimica, Instituto de Ciencias Basicas da Saude, UFRGS, Rua Ramiro Barcelos, 2600 Anexo, CEP 90035 003 Porto Alegre, RS, Brazil
    Cancer Lett 198:211-8. 2003
    ....
  45. pmc Bateman domains and adenosine derivatives form a binding contract
    Bruce E Kemp
    St Vincent s Institute of Medical Research, Fitzroy, Victoria, Australia
    J Clin Invest 113:182-4. 2004
    ....
  46. pmc Characterization of AMP-activated protein kinase gamma-subunit isoforms and their role in AMP binding
    P C Cheung
    Cellular Stress Group, MRC Clinical Sciences Centre, Imperial College School of Medicine, Hammersmith Hospital, Du Cane Road, London W12 0NN, U K
    Biochem J 346:659-69. 2000
    ..Labelling studies, using the reactive AMP analogue 8-azido-[(32)P]AMP, indicate that the gamma subunit may participate directly in the binding of AMP within the complex...
  47. ncbi The AMP-activated/SNF1 protein kinase subfamily: metabolic sensors of the eukaryotic cell?
    D G Hardie
    Biochemistry Department, University, Dundee, Scotland, United Kingdom
    Annu Rev Biochem 67:821-55. 1998
    ..SNF1-related protein kinases in higher plants are likely to be involved in the response of plant cells to environmental and/or nutritional stress...
  48. ncbi Structure-based analysis of catalysis and substrate definition in the HIT protein family
    C D Lima
    Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032, USA
    Science 278:286-90. 1997
    ..The approach described here may be useful in identifying structure-function relations between protein families identified through genomics...
  49. pmc Poly(ADP-ribose) catabolism triggers AMP-dependent mitochondrial energy failure
    Laura Formentini
    Department of Preclinical and Clinical Pharmacology, University of Florence, 50139 Firenze, Italy
    J Biol Chem 284:17668-76. 2009
    ..Also, these data disclose unanticipated AMP-dependent impairment of mitochondrial exchange of adenine nucleotides, which can be of relevance to organelle functioning and disease pathogenesis...
  50. ncbi Retinoic acid receptor-related orphan receptor α-induced activation of adenosine monophosphate-activated protein kinase results in attenuation of hepatic steatosis
    Eun Jin Kim
    College of Pharmacy, Seoul National University, Seoul, Korea
    Hepatology 55:1379-88. 2012
    ..Here, we report that RORα attenuates hepatic steatosis, probably via activation of the adenosine monophosphate (AMP)-activated protein kinase (AMPK) and repression of the liver X receptor α (LXRα)...
  51. ncbi Muscle FBPase in a complex with muscle aldolase is insensitive to AMP inhibition
    D Rakus
    Department of Animal Physiology, Institute of Zoology, Wroclaw University, Cybulskiego 30, 50 205, Wroclaw, Poland
    FEBS Lett 547:11-4. 2003
    ..The existence of an FBPase-aldolase complex that is insensitive to AMP inhibition explains the possibility of glycogen synthesis from carbohydrate precursors in vertebrates' myocytes...
  52. ncbi Molecular dynamics of apo-adenylate kinase: a distance replica exchange method for the free energy of conformational fluctuations
    Hongfeng Lou
    Department of Chemistry and the Quantitative Biology Modeling Initiative, Michigan State University, East Lansing, Michigan 48824 1322, USA
    J Phys Chem B 110:24121-37. 2006
    ..Therefore, apo AKE can fluctuate from its open form to conformations that are quite similar to its closed form X-ray structure, even in the absence of its substrates...
  53. ncbi Structures of thermophilic and mesophilic adenylate kinases from the genus Methanococcus
    Angela R Criswell
    Department of Biochemistry and Cell Biology, Rice University, Houston, TX 77005, USA
    J Mol Biol 330:1087-99. 2003
    ..Results of previous mutational studies are interpreted in terms of the crystal structures, and support the importance of hydrophobic interactions in thermostability...
  54. ncbi Butterbur, a herbal remedy, attenuates adenosine monophosphate induced nasal responsiveness in seasonal allergic rhinitis
    D K C Lee
    Asthma and Allergy Research Group, Ninewells Hospital and Medical School, University of Dundee, Dundee, Scotland, UK
    Clin Exp Allergy 33:882-6. 2003
    ..However, no placebo-controlled studies have been performed to evaluate the effectiveness of BB on objective outcomes such as nasal provocation testing in seasonal allergic rhinitis (SAR)...
  55. ncbi Structure of E69Q mutant of human muscle fructose-1,6-bisphosphatase
    Marek Zarzycki
    Institute of Genetics and Microbiology, Faculty of Biological Sciences, University of Wroclaw, ul Przybyszewskiego 63 77, 51 148 Wroclaw, Poland
    Acta Crystallogr D Biol Crystallogr 67:1028-34. 2011
    ..The AMP binding pattern of the muscle isozyme is quite similar to that of the liver isozyme and the T conformations of the two isozymes are nearly the same...
  56. ncbi Structural insights into the architecture and allostery of full-length AMP-activated protein kinase
    Li Zhu
    MOE Key Laboratory of Arid and Grassland Ecology, Institute of Biophysics, Lanzhou University, Lanzhou 730000, P R China
    Structure 19:515-22. 2011
    ..These results not only make substantial revision to the current model of AMPK assembly, but also highlight a central role of the linker sequence of the α subunit in mediating the allostery of AMPK...
  57. ncbi Synthesis and biodistribution of [11C]adenosine 5'-monophosphate ([11C]AMP)
    William B Mathews
    Division of Nuclear Medicine, Johns Hopkins University, Room B1151 Nelson Building, 600 N Wolfe St, Baltimore, MD 21287, USA
    Mol Imaging Biol 7:203-8. 2005
    ..In order to develop a tracer for positron emission tomography (PET) that would not be subject to loss of its radioisotope, adenosine 5'-monophosphate (AMP) was intrinsically labeled at the C-8 position with carbon-11...
  58. ncbi Mimicry of a cellular low energy status blocks tumor cell anabolism and suppresses the malignant phenotype
    Johannes V Swinnen
    Laboratory for Experimental Medicine and Endocrinology, University of Leuven, Leuven, Belgium
    Cancer Res 65:2441-8. 2005
    ..These findings point toward a central tie between energy, anabolism, and cancer and suggest that the cellular energy sensing machinery in cancer cells is an exploitable target for cancer prevention and/or therapy...
  59. ncbi Apo adenylate kinase encodes its holo form: a principal component and varimax analysis
    Robert I Cukier
    Department of Chemistry and the Quantitative Biology Modeling Initiative, Michigan State University, East Lansing, Michigan 48824, USA
    J Phys Chem B 113:1662-72. 2009
    ..In this sense, apo AKE does encode in its fluctuations information about holo-like conformations...
  60. ncbi Inhibition of fructose-1,6-bisphosphatase by aminoimidazole carboxamide ribotide prevents growth of Salmonella enterica purH mutants on glycerol
    Michael J Dougherty
    Department of Bacteriology, University of Wisconsin, Madison, Wisconsin 53706, USA
    J Biol Chem 281:33892-9. 2006
    ..Inhibition of FBP activity by AICAR occurs at physiologically relevant concentrations and may represent a form of regulation of gluconeogenic flux in Salmonella enterica...
  61. pmc Ecto-5'-nucleotidase (CD73) inhibits nociception by hydrolyzing AMP to adenosine in nociceptive circuits
    Nathaniel A Sowa
    Department of Cell and Molecular Physiology, UNC Neuroscience Center and Curriculum in Neurobiology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    J Neurosci 30:2235-44. 2010
    ..Moreover, our data suggest NT5E is well localized to regulate nucleotide signaling between skin cells and sensory axons...
  62. ncbi Structural basis for the recognition of isoleucyl-adenylate and an antibiotic, mupirocin, by isoleucyl-tRNA synthetase
    T Nakama
    Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    J Biol Chem 276:47387-93. 2001
    ..Mutagenic analyses revealed that the replacement of the two residues significantly changed the sensitivity to mupirocin...
  63. ncbi AMP does not induce torpor
    Steven J Swoap
    Department of Biology, Williams College, Williamstown, MA 01267, USA
    Am J Physiol Regul Integr Comp Physiol 293:R468-73. 2007
    ..These data suggest that injection of adenine nucleotides into mice induces a reversible hypothermic state that is unrelated to fasting-induced torpor...
  64. ncbi Novel allosteric activation site in Escherichia coli fructose-1,6-bisphosphatase
    Justin K Hines
    Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, Ames, Iowa 50011, USA
    J Biol Chem 281:18386-93. 2006
    ....
  65. pmc Demonstration of phosphoryl group transfer indicates that the ATP-binding cassette (ABC) transporter cystic fibrosis transmembrane conductance regulator (CFTR) exhibits adenylate kinase activity
    Christoph O Randak
    Department of Pediatrics, University of Iowa, Iowa City, Iowa 52242, USA
    J Biol Chem 287:36105-10. 2012
    ..These biochemical data complement earlier biophysical studies of CFTR and indicate that the ABC transporter CFTR can function as an adenylate kinase...
  66. ncbi The origin of the high sensitivity of muscle fructose 1,6-bisphosphatase towards AMP
    D Rakus
    Department of Animal Physiology, Institute of Zoology, Wroclaw University, Poland
    FEBS Lett 579:5577-81. 2005
    ..High affinity to the inhibitor but low sensitivity to AMP of the liver triple mutant suggest differences between the isozymes in the mechanism of allosteric signal transmission...
  67. ncbi Structures of mammalian and bacterial fructose-1,6-bisphosphatase reveal the basis for synergism in AMP/fructose 2,6-bisphosphate inhibition
    Justin K Hines
    Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, Ames, Iowa 50011, USA
    J Biol Chem 282:36121-31. 2007
    ..Conceivably, Type I FBPases from all eukaryotes may undergo similar global conformational changes in response to Fru-2,6-P(2) ligation...
  68. ncbi An intrinsic adenylate kinase activity regulates gating of the ABC transporter CFTR
    Christoph Randak
    Howard Hughes Medical Institute, Department of Internal Medicine and Physiology, Roy J and Lucille A Carver College of Medicine, University of Iowa, Iowa City, IA 52242, USA
    Cell 115:837-50. 2003
    ..At physiologic nucleotide concentrations, adenylate kinase activity, rather than ATPase activity may control gating, and therefore involve little energy consumption...
  69. ncbi Effect of adenine nucleotide pool size in mitochondria on intramitochondrial ATP levels
    L Schild
    Institut fur Klinische Chemie, Medizinische Fakultät der Otto von Guericke Universität Magdeburg, Germany
    Biochim Biophys Acta 1413:14-20. 1999
    ..In particular, this could be the case within the postischemic phase which is characterized by low cytosolic adenine nucleotide concentrations and energized mitochondria...
  70. pmc When hypothermia meets hypotension and hyperglycemia: the diverse effects of adenosine 5'-monophosphate on cerebral ischemia in rats
    Feng Zhang
    Geriatric Research, Educational and Clinical Center, Veterans Affairs Pittsburgh Health Care System, Pittsburgh, Pennsylvania, USA
    J Cereb Blood Flow Metab 29:1022-34. 2009
    ....
  71. ncbi Constant darkness is a circadian metabolic signal in mammals
    Jianfa Zhang
    Department of Biochemistry and Molecular Biology, University of Texas Health Science Center, Houston, Texas 77030, USA
    Nature 439:340-3. 2006
    ..Induction of mClps expression by 5'-AMP in LD mice was reciprocally linked to blood glucose levels. Our findings uncover a circadian metabolic rhythm in mammals...
  72. pmc The AMP-activated protein kinase AAK-2 links energy levels and insulin-like signals to lifespan in C. elegans
    Javier Apfeld
    Elixir Pharmaceuticals, Cambridge, Massachusetts 02139, USA
    Genes Dev 18:3004-9. 2004
    ..Thus, AAK-2 is a sensor that couples lifespan to information about energy levels and insulin-like signals...
  73. ncbi Structure of inhibited fructose-1,6-bisphosphatase from Escherichia coli: distinct allosteric inhibition sites for AMP and glucose 6-phosphate and the characterization of a gluconeogenic switch
    Justin K Hines
    Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, Ames, Iowa 50011, USA
    J Biol Chem 282:24697-706. 2007
    ..FBPases in such organisms may be components of metabolic switches that allow rapid changeover between gluconeogenesis and glycolysis in response to nutrient availability...
  74. ncbi Bronchial hyperresponsiveness to methacholine and adenosine monophosphate and the degree of atopy in children with allergic rhinitis
    Chang Keun Kim
    Asthma and Allergy Center, Inje University Sanggye Paik Hospital, Seoul, Korea
    Ann Allergy Asthma Immunol 106:36-41. 2011
    ..Not only the presence but also the degree of atopy are important factors in BHR of patients with asthma. BHR is commonly evaluated by bronchial challenges using direct or indirect stimuli...
  75. ncbi Dose-response comparison of budesonide dry powder inhalers using adenosine monophosphate bronchial challenge
    Brian J Lipworth
    Asthma and Allergy Research Group, Division of Medicine and Therapeutics, Ninewells University Hospital and Medical School, University of Dundee, Dundee, Scotland
    Ann Allergy Asthma Immunol 94:675-81. 2005
    Bronchial hyperresponsiveness to adenosine monophosphate, an indirect measure of airway inflammation, is a sensitive marker of inhaled corticosteroid efficacy.
  76. ncbi Role of adenosine monophosphate-activated protein kinase in the control of energy homeostasis
    Fabienne Foufelle
    INSERM Unit 671, University Pierre et Marie Curie, Centre de Recherches Biomedicales des Cordeliers, Paris, France
    Curr Opin Clin Nutr Metab Care 8:355-60. 2005
    b>Adenosine monophosphate-activated protein kinase is involved in cellular energy homeostasis...
  77. pmc Biochemical, crystallographic, and mutagenic characterization of hint, the AMP-lysine hydrolase, with novel substrates and inhibitors
    Agnieszka Krakowiak
    Structural Biology and Bioinformatics Program, Kimmel Cancer Center, Philadelphia, Pennsylvania 19107, USA
    J Biol Chem 279:18711-6. 2004
    ..Ser-107 is positioned to donate a hydrogen bond to the leaving group nitrogen. Consistent with a role in acid-base catalysis, the Hint S107A mutant protein displayed depressed catalytic activity...
  78. pmc An evaluation of short-term corticosteroid response in perennial allergic rhinitis using histamine and adenosine monophosphate nasal challenge
    Andrew M Wilson
    Asthma and Allergy Research Group, Ninewells Hospital and Medical School, University of Dundee, Dundee DD1 9SY, Scotland, UK
    Br J Clin Pharmacol 55:354-9. 2003
    ..b>Adenosine monophosphate (AMP) challenge has been shown to be a good inflammatory surrogate in the lower airways, but it has not ..
  79. pmc Altered specificity of Hint-W123Q supports a role for Hint inhibition by ASW in avian sex determination
    Kristen P Parks
    Department of Genetics, Norris Cotton Cancer Center, Dartmouth Medical School, Lebanon, New Hampshire 03756, USA
    Physiol Genomics 20:12-4. 2004
    ..The resulting 2,700-fold switch in enzyme specificity suggests that Gln127 could be the dominant component of Asw dominant negativity in avian feminization...
  80. ncbi Identification and characterization of a cell-surface receptor, P2Y15, for AMP and adenosine
    Hisayo Inbe
    Bayer Yakuhin, Ltd, Research Center Kyoto, 6 5 1 3 Kunimidai, Kizu cho, Soraku gun, Kyoto 619 0216, Japan
    J Biol Chem 279:19790-9. 2004
    ..We found inhibition at a theophylline concentration well within the therapeutic dose range, indicating that P2Y15 may be a clinically important target of this drug...
  81. ncbi Stimulation of adenosine A1 and A2A receptors by AMP in the submucosal plexus of guinea pig small intestine
    Na Gao
    Dept of Physiology and Cell Biology, Columbus, OH 43210 1218, USA
    Am J Physiol Gastrointest Liver Physiol 292:G492-500. 2007
    ....
  82. pmc Treatment of heart failure by a methanocarba derivative of adenosine monophosphate: implication for a role of cardiac purinergic P2X receptors
    Si Yuan Zhou
    The Pat and Jim Calhoun Cardiology Center, University of Connecticut Health Center, Farmington, Connecticut 06030, USA
    J Pharmacol Exp Ther 333:920-8. 2010
    ..We developed a hydrolysis-resistant adenosine monophosphate derivative, (1'S,2R,3S,4'R,5'S)-4-(6-amino-2-chloro-9H-purin-9-yl)-1-[phosphoryloxymethyl] bicycle[3.1...
  83. ncbi Provocation with adenosine 5'-monophosphate as a marker of inflammation in asthma, allergic rhinitis and chronic obstructive pulmonary disease
    M van den Berge
    Department of Pulmonology, University Hospital Groningen, Groningen, The Netherlands
    Clin Exp Allergy 32:824-30. 2002
  84. pmc Recombinant ecto-5'-nucleotidase (CD73) has long lasting antinociceptive effects that are dependent on adenosine A1 receptor activation
    Nathaniel A Sowa
    Department of Cell and Molecular Physiology, UNC Neuroscience Center, University of North Carolina, CB 7545, Chapel Hill, North Carolina 27599, USA
    Mol Pain 6:20. 2010
    ..Since adenosine has antinociceptive effects in rodents and humans, we hypothesized that NT5E, an enzyme that generates adenosine, might also have antinociceptive effects in vivo...
  85. ncbi A novel transverse push-pull microprobe: in vitro characterization and in vivo demonstration of the enzymatic production of adenosine in the spinal cord dorsal horn
    S L Patterson
    Neuroscience Graduate Program, The University of Iowa, Iowa City, Iowa 52242, USA
    J Neurochem 76:234-46. 2001
    ....
  86. pmc Histidine triad nucleotide-binding protein 1 (HINT-1) phosphoramidase transforms nucleoside 5'-O-phosphorothioates to nucleoside 5'-O-phosphates
    Magdalena Ozga
    Department of Bioorganic Chemistry, Centre of Molecular and Macromolecular Studies, Polish Academy of Sciences, Lodz 90 363, Poland
    J Biol Chem 285:40809-18. 2010
    ....
  87. ncbi Relationship of skin-prick reactivity to aeroallergens and hyperresponsiveness to challenges with methacholine and adenosine monophosphate
    Stephen J Fowler
    Asthma and Allergy Research Group, Ninewells Hospital and Medical School, University of Dundee, Scotland
    Allergy 58:46-52. 2003
    ..We therefore investigated the putative relationship between skin-prick reactivity to aeroallergens and BHR to direct and indirect stimuli...
  88. ncbi Adenosine 5'-monophosphate in asthma: gas exchange and sputum cellular responses
    H A Manrique
    Servei de Pneumologia Institut del Tòrax, Hospital Clinic, Institut d Investigacions Biomediques August Pi i Sunyer, CIBER Enfermedades Respiratorias, Universitat de Barcelona, Barcelona, Spain
    Eur Respir J 31:1205-12. 2008
    ..The fully inhibited adenosine 5'-monophosphate-induced neutrophilia with residual hypoxaemia observed after salbutamol treatment is probably related to beta(2)-agonists acting on both bronchial and pulmonary circulation...
  89. ncbi Structural and catalytic role of arginine 88 in Escherichia coli adenylate kinase as evidenced by chemical modification and site-directed mutagenesis
    J Reinstein
    Max Planck Institut fur medizinische Forschung, Abteilung Biophysik, Heidelberg, West Germany
    J Biol Chem 264:8107-12. 1989
    ..It possibly stabilizes the transferable gamma-phosphate group from ATP to AMP in the transition state...
  90. ncbi Towards a mechanism of AMP-substrate inhibition in adenylate kinase from Escherichia coli
    M A Sinev
    Department of Life Sciences, Bar Ilan University, Ramat Gan, Israel
    FEBS Lett 397:273-6. 1996
    ..Closure of the LID domain in response to AMP binding may be a possible reason for the strong AMP-substrate inhibition known for E. coli AK...
  91. ncbi Influence of ischemic injury on vein graft remodeling: role of cyclic adenosine monophosphate second messenger pathway in enhanced vein graft preservation
    Taichi Sakaguchi
    Department of Surgery, College of Physicians and Surgeons, Columbia University, New York, NY, USA
    J Thorac Cardiovasc Surg 129:129-37. 2005
    ..of vein grafts was linked to the later development of graft neointimal hyperplasia and whether the restoration of the cyclic adenosine monophosphate second messenger pathway would attenuate the development of neointimal hyperplasia.
  92. pmc Safety of hookworm infection in individuals with measurable airway responsiveness: a randomized placebo-controlled feasibility study
    J Feary
    Division of Epidemiology and Public Health, University of Nottingham, UK
    Clin Exp Allergy 39:1060-8. 2009
    ....
  93. ncbi Measurements of ATP in mammalian cells
    Giovanni Manfredi
    Department of Neurology and Neuroscience, Weill Medical College of Cornell University, 525 East 68th Street, A 505, New York, USA
    Methods 26:317-26. 2002
    ..Finally, we describe an HPLC-based method for accurate measurement of ATP, ADP, and AMP in cultured cells and animal tissues...
  94. ncbi Making AppDNA using T4 DNA ligase
    William Chiuman
    Department of Biochemistry Department of Chemistry, Health Sciences Centre, McMaster University, 1200 Main Street West, Hamilton, Ont, Canada L8N 3Z5
    Bioorg Chem 30:332-49. 2002
    ..We further found that a donor-template duplex of 11-bp in length is required by T4 DNA ligase for the formation of AppDNA...
  95. ncbi Probing the impact of the echinT C-terminal domain on structure and catalysis
    Sanaa Bardaweel
    Department of Medicinal Chemistry, University of Minnesota, 8 174 Weaver Densford Hall, 308 Harvard Street SE, Minneapolis, MN 55455, USA
    J Mol Biol 404:627-38. 2010
    ..Taken together, our results highlight the emerging role of the C-terminus in governing the catalytic function of Hints...
  96. ncbi Crystal structures of biotin protein ligase from Pyrococcus horikoshii OT3 and its complexes: structural basis of biotin activation
    Bagautdin Bagautdinov
    Advanced Protein Crystallography Research Group, RIKEN Harima Institute at SPring 8, 1 1 1 Kouto, Mikazuki cho, Sayo gun, Hyogo 679 5148, Japan
    J Mol Biol 353:322-33. 2005
    ....
  97. ncbi The Legionella effector protein DrrA AMPylates the membrane traffic regulator Rab1b
    Matthias P Müller
    Department of Physical Biochemistry, Max Planck Institute of Molecular Physiology, Dortmund, NRW, 44227, Germany
    Science 329:946-9. 2010
    ..AMPylation of switch II by DrrA restricts the access of GTPase activating proteins, thereby rendering Rab1b constitutively active...
  98. ncbi A 54-kDa fragment of the Poly(A)-specific ribonuclease is an oligomeric, processive, and cap-interacting Poly(A)-specific 3' exonuclease
    J Martinez
    Department of Cell and Molecular Biology, Uppsala University, Box 596, SE 751 24 Uppsala, Sweden
    J Biol Chem 275:24222-30. 2000
    ..Addition of free m(7)G(5')ppp(5')G cap analogue inhibited poly(A) degradation in vitro, suggesting a functional link between the RNA 5' end cap structure and poly(A) degradation at the 3' end of the RNA...
  99. pmc Berberine improves glucose metabolism through induction of glycolysis
    Jun Yin
    Pennington Biomedical Research Center, Louisiana State University System, 6400 Perkins Rd, Baton Rouge, LA 70808, USA
    Am J Physiol Endocrinol Metab 294:E148-56. 2008
    ..Berberine-induced AMPK activation is likely a consequence of mitochondria inhibition that increases the AMP/ATP ratio...
  100. ncbi Human monocyte derived dendritic cells express functional P2X and P2Y receptors as well as ecto-nucleotidases
    S Berchtold
    Department of Dermatology, University of Erlangen, Germany
    FEBS Lett 458:424-8. 1999
    ..Extracellular ATP was rapidly hydrolyzed by these ecto-enzymes as shown by separation of 3H-labeled ATP metabolites using a thin layer technique. These data suggest that ATP acts as a costimulatory factor on DC maturation...
  101. ncbi The role of endogenous and exogenous AMP in asthma and chronic obstructive pulmonary disease
    Maarten van den Berge
    Department of Pulmonology, University Hospital Groningen, The Netherlands
    J Allergy Clin Immunol 114:737-46. 2004
    ..The aim of this article was to review the literature assessing AMP's value in asthma and chronic obstructive pulmonary disease...

Research Grants63

  1. Role of Chromogranin A in Metabolic Syndrome
    Sushil K Mahata; Fiscal Year: 2013
    ..stimulates gluconeogenesis by attenuating endothelial nitric oxide synthase (eNOS) and 5'-adenosine monophosphate-activated protein kinase (AMPK), and enhances glucose disposal and glycogen storage by preventing ..
  2. Med1 in Liver Metabolism, Regeneration and Cancer
    Janardan K Reddy; Fiscal Year: 2013
    ..Our preliminary data indicate that Med1 is phosphorylated by energy sensor, adenosine monophosphate (AMP)-activated protein kinase (AMPK)...
  3. Regulation of microRNA-375 by cyclic-AMP in pancreatic beta-cells
    DAVID MATTHEW KELLER; Fiscal Year: 2010
    DESCRIPTION (provided by applicant): The cyclic-adenosine monophosphate (cAMP) second messenger signaling cascade is a key regulator of diverse cellular process such as insulin secretion and energy homeostasis (Seino and Shibasaki, 2005)..
  4. Respiration in Sepsis
    Claude A Piantadosi; Fiscal Year: 2013
    ..biogenesis in sepsis is activated before energy crisis, while a rescue pathway is delayed involving the adenosine monophosphate (AMP)-activated protein kinase (AMPK) and PGC-1[unreadable] co-activator...
  5. Fic-mediated Adenylylation
    CAROLYN ANN WORBY; Fiscal Year: 2013
    ..Fic domains of IbpA block signaling of these GTPases by using ATP to catalyze the covalent addition of adenosine monophosphate (AMP) to a tyrosine (Tyr) residue in the GTPase switch I region...
  6. Impact of AMP-activated kinase on sex differences in hypertrophic cardiomyopathy
    John P Konhilas; Fiscal Year: 2013
    ..b>Adenosine monophosphate-activated kinase (AMPK) may be a central regulator of this sex difference because of its established role ..
  7. Pulmonary Vascular-Right Ventricular Axis Research Program
    Wai Hong Wilson Tang; Fiscal Year: 2013
    ..at Threonine 495 (T495) and phosphorylation at Serine 1177 (S1177), the latter mediated by cyclic adenosine monophosphate (cAMP)/protein kinase pathways downstream of ?-Adrenergic Receptors (?AR) [Fig 1]...
  8. Development of inhibitors of AMP Deaminase Isoform 2 as a Mechanism for Treating
    RICHARD JOSEPH JOHNSON; Fiscal Year: 2010
    ..The goal of the project is to develop the first selective adenosine monophosphate deaminase- 2 (AMPD2) inhibitor, which we believe will offer an important new method of treatment for ..
  9. Sympathetic Regulation of AMPK in the control of non-shivering thermogenesis
    Jacob D Mulligan; Fiscal Year: 2012
    While a role for adenosine monophosphate activated protein kinase (AMPK) in regulating body energy balance via effects in non-adipose tissues has gained acceptance, little is known about the biological function of AMPK in white and ..
  10. The Role of Leptin in Liver Fibrogenesis
    Frank A Anania; Fiscal Year: 2012
    ..and, this inhibitory activity requires the adiponectin R1 receptor but does not appear to depend on adenosine monophosphate kinase (AMPK)...
  11. Multiple Molecular Mechanisms for Adiponectin Therapy in Hepatic Fibrogenesis
    Frank A Anania; Fiscal Year: 2013
    ..pro-fibrogenic responses and required adiponectin receptor 1, but does not appear to depend entirely on adenosine monophosphate kinase (AMPK)...
  12. Hepatocyte Nitric Oxide Synthase Regulation by Glucagon and Insulin
    Brian G Harbrecht; Fiscal Year: 2012
    ..Excessive NO from iNOS produces cellular dysfunction and hepatic injury. Glucagon and cyclic adenosine monophosphate (cAMP) regulate hepatic iNOS expression in vitro and in vivo, and by doing so, decrease NO-mediated ..
  13. Impact of AMP-activated kinase on sex differences in hypertrophic cardiomyopathy
    John P Konhilas; Fiscal Year: 2013
    ..b>Adenosine monophosphate-activated kinase (AMPK) may be a central regulator of this sex difference because of its established role ..
  14. Insulin Clearance: Candidate and Positional Genetic Determinants
    MARK GOODARZI; Fiscal Year: 2012
    ..Insulin clearance is a highly heritable trait. Preliminary data show that haplotypes in the genes for adenosine monophosphate deaminase (AMPD1 and AMPD2) and the AMP-activated protein kinase (AMPK) 12 subunit are associated with ..
  15. Molecular mechanisms of neural plasticity in drug addiction
    LAURA NICOLE SMITH; Fiscal Year: 2010
    ..exposure regulates spine density in the nucleus accumbens, the brain's reward center, via cyclic-adenosine monophosphate (cAMP)-dependent inhibition of a transcription factor known as myocyte enhancer factor 2 (MEF2)...
  16. Angiotensin II, IGF-1 and Skeletal Muscle Atrophy
    Patrice Delafontaine; Fiscal Year: 2013
    ..insulin and IGF-1 signaling, induces mitochondrial dysfunction, depletes muscle ATP, and inhibits 5'-Adenosine Monophosphate Activated Protein Kinase (AMPK) signaling, preventing the normal response to energy depletion...
  17. Novel AMPK Activators in Breast Cancer Prevention
    Ching Shih Chen; Fiscal Year: 2012
    DESCRIPTION (provided by applicant): Accumulating evidence suggests a link between adenosine monophosphate-activated protein kinase (AMPK), a well known regulator of energy homeostasis and insulin sensitivity, and cancer cell growth and ..
  18. Nitric oxide and mitochondrial biogenesis in sepsis
    Claude A Piantadosi; Fiscal Year: 2012
    ..in the regulation of hepatic mitochondrial biogenesis through the major co-activator, PGC-11 and the adenosine monophosphate (AMP)-activated protein kinase (AMPK)...
  19. VIP-induced gene expression in colonic smooth muscle cells
    Sushil K Sarna; Fiscal Year: 2010
    ..to their receptors on smooth muscle cells, activate adenylate cyclase to produce cyclic 3'-5'adenosine monophosphate (cAMP) that mediates smooth muscle relaxation...
  20. DEVELOPING PET AGENTS FOR IMAGING PHOSPHODIESTERASE 10A (PDE10A)
    Zhude Tu; Fiscal Year: 2012
    ..where it plays a critical role in the regulation of both cyclic guanosine monophosphate (cGMP) and cyclic adenosine monophosphate (cAMP)...
  21. M. Tuberculosis Influences Host Matrix Metalloproteinases and Granuloma Formation
    NICOLE AMMERMAN; Fiscal Year: 2013
    ..tuberculosis-encoded adenylate cyclase (Rv0386) responsible for the production of 3', 5'-cyclic adenosine monophosphate (cAMP) that is directly injected into the host cell, and preliminary data indicate that this ..
  22. HuR Regulation of HSP 70 in Brain Reperfusion
    JEFFREY SZYMANSKI; Fiscal Year: 2013
    ..2. To determine if HuR nuclear export is causally linked to HSP70 translation. 5'adenosine monophosphate- activated protein kinase (AMPK) activation causes nuclear retention of HuR and worsens stroke outcome...
  23. NIH Director's Pioneer Award
    Cheng C Lee; Fiscal Year: 2010
    ..We have recently identified endogenous 5?-adenosine monophosphate (5?-AMP) as a mediator of torpor behavior in mice...
  24. DEVELOPING PET AGENTS FOR IMAGING PHOSPHODIESTERASE 10A (PDE10A)
    Zhude Tu; Fiscal Year: 2013
    ..where it plays a critical role in the regulation of both cyclic guanosine monophosphate (cGMP) and cyclic adenosine monophosphate (cAMP)...
  25. Dopamine Signaling Mechanisms of Traumatic Brain Injury
    C Edward Dixon; Fiscal Year: 2013
    ..The DA- and cyclic adenosine monophosphate (cAMP)-regulated phosphoprotein, Mr 32 kDA (DARPP-32), is a key convergence point in striatal medium ..
  26. The Roles of Host Factors in the AMPylation Activity of a Bacterial Effector
    Zhao Qing Luo; Fiscal Year: 2012
    ..described AMPylation factors, a conserved functional motif called Fic (filamentation induced by cyclic adenosine monophosphate) with the sequences (HPFx[D/E]GN[G/K]R) catalyzes the enzymatic reaction whereby the invariant histidine ..
  27. The Role of Cyclic AMP in Alcohol Withdrawal and Mental Disease
    JULIA ANN CHESTER; Fiscal Year: 2010
    ..Evidence suggests that activating the 3',5'-cyclic adenosine monophosphate (cAMP) second messenger signaling pathway may reduce the behavioral effects of alcohol withdrawal...
  28. A peripheral diagnostic biomarker for Major Depressive Disorder
    Lee Schechter; Fiscal Year: 2013
    ..the localization of Gs[unreadable], a crucial G protein that activates adenylyl cyclase to produce cyclic adenosine monophosphate (cAMP) and initiate downstream events...
  29. Aging and Arterial Dysfunction with a Western Diet: Exercise and AMPK
    Douglas R Seals; Fiscal Year: 2013
    ..5. Increased arterial expression and/or activity of adenosine monophosphate activated protein kinase (AMPK) will contribute to these protective effects of exercise...
  30. AMPK activates calcium signaling necessary for endothelial barrier repair
    Judy R Creighton; Fiscal Year: 2013
    ..In this application, we propose a novel role for adenosine monophosphate kinase (AMPK) in promoting cadherin adhesion assembly critical for endothelial repair...
  31. NEURAL PATHWAYS FOR METABOLIC CONTROL OF INGESTION
    W SUE RITTER; Fiscal Year: 2013
    ..Finally, the proposed work will examine the contribution of 5'adenosine monophosphate-activated protein kinase (AMPK), a cellular energy sensor and regulator, to the function of hindbrain NE/..
  32. Role of receptor trafficking in synaptic plasticity during morphine dependence
    Anuradha Madhavan; Fiscal Year: 2013
    ..Withdrawal from chronic morphine results in a cyclic adenosine monophosphate (cAMP)-dependent increased probability of gamma-aminobutyric acid (GABA) release in ventral tegmental ..
  33. A Systems Biology Approach To Cardiomyopathy in the D. Melanogaster Model System
    BRIAN O'ROURKE; Fiscal Year: 2013
    ..cardiomyopathy mutant Mhc5 shows substantial metabolic remodeling, suggesting a possible role for adenosine monophosphate activated protein kinase (AMPK)...
  34. AMPK ACTIVATORS FOR THE TREATMENT OF POST-SURGICAL PAIN
    Theodore J Price; Fiscal Year: 2013
    ..effort is to further develop two potential mechanisms for the pharmacological manipulation of pain: adenosine monophosphate activated kinase (AMPK) activators and peptides that disrupt voltage-gated sodium channel type 1.7 (Nav1...
  35. MAST CELL MEMBRANE GLYCOLIPID EXPRESSION AND FUNCTION
    Michael Raizman; Fiscal Year: 1991
    ..mast cells ex vivo; and to determine if the effects of cholera toxin on the histamine and cyclic adenosine monophosphate contents of these populations correlate with ganglioside GM1 expression...
  36. Action of ethanol on cyclic AMP signal transduction
    Masami Yoshimura; Fiscal Year: 2013
    ..is to elucidate the molecular and cellular mechanisms underlying the effects of ethanol on the cyclic adenosine monophosphate (cAMP) signaling pathway in the central nervous system...
  37. REGULATION OF MAMMALIAN OOCYTE DEVELOPMENT
    John Eppig; Fiscal Year: 1993
    ..There is strong evidence that elevated oocyte cyclic adenosine monophosphate (cAMP) levels are physiologically important for maintaining oocyte meiotic arrest...
  38. SLEEP MODULATION OF EPILEPTIC ACTIVITY IN IMMATURE BRAIN
    Anne Anderson; Fiscal Year: 2000
    ..As a result, intracellular cyclic adenosine monophosphate (cAMP) levels increase. This in turn activates cAMP dependent protein kinase (PKA)...
  39. REGULATION OF SYNAPTIC TRANSMISSION
    Robert Zucker; Fiscal Year: 2002
    ..and long-term depression (LTD) in the CA1 region of mammalian hippocampal cortex? The roles of cyclic adenosine monophosphate dependent protein kinase and Ca2+/calmodulin dependent protein kinase will be evaluated as determinants ..
  40. MEMBRANE TOPOLOGY AND BIOSYNTHESIS OF GLYCOSAMINOGLYCANS
    CARLOS HIRSCHBERG; Fiscal Year: 2002
    ..Together, the above approaches should continue to enable us to better understand the role of proteoglycans in their many biological roles. ..
  41. ROLE OF G PROTEINS IN PROLACTIN SIGNAL TRANSDUCTION
    Jennifer Larsen; Fiscal Year: 1993
    ..actions on the Nb2 cell through generation of increased cellular concentrations of cyclic 3'5'- adenosine monophosphate (cAMP)...
  42. New Mechanisms in the Pathogenesis of Cholera
    JOHNNY PETERSON; Fiscal Year: 2006
    ..upregulate adenylate cyclase activity by catalyzing the ADP-ribosylation of Gs-alpha and increase 3, 5 adenosine monophosphate (cAMP) levels, equally important are stimulatory effects on AA metabolism leading to increased production ..
  43. Real-time measurement of ethanol's effect on cyclic AMP metabolism in live cells
    Masami Yoshimura; Fiscal Year: 2009
    ..is to elucidate the molecular and cellular mechanisms underlying the effects of ethanol on the cyclic adenosine monophosphate (cAMP) signaling pathway in the central nervous system...
  44. NE Signal Transduction in Classical Conditioning
    Paula Bickford; Fiscal Year: 2009
    ..signal transduction cascades involved in learning and memory, including the clear implication of cyclic adenosine monophosphate (cAMP)-dependent protein kinase (PKA) in both long and short-term memory formation in numerous parts of ..
  45. GUIDANCE MOLECULES IN SPINAL CORD REGENERATION
    MICHAEL SELZER; Fiscal Year: 2009
    ..can be converted to attraction by manipulation of the cyclic guanosine-monophosphate(cGMP) and cyclic adenosine monophosphate (cAMP) signaling pathways, we will determine the effect of altering intracellular cAMP and/or cGMP ..
  46. Very Low Naltrexone Treatment of Opiate Withdrawal
    Elisabeth Van Bockstaele; Fiscal Year: 2005
    ..Furthermore, alterations in intracellular messengers, including cyclic adenosine monophosphate (cAMP)-dependent protein kinases (PKA), and cAMP-response element-binding protein (CREB) have been shown ..
  47. Altered phosphodiesterase expression in diseased muscle
    TIMOTHY BLOOM; Fiscal Year: 2007
    ..Cyclic 3',5'- adenosine monophosphate (cAMP) is a universally utilized intracellular second messenger...
  48. MEMBRANE MORPHOGENESIS AND DIFFERENTIATION IN BACTERIA
    ROBERT UFFEN; Fiscal Year: 1980
    ..The observation that cyclic-3',5'-adenosine monophosphate alters the diauxie in a predictable way has suggested that catabolite repression, or a closely related ..
  49. REGULATION OF PARATHYROID HORMONE ACTION IN KIDNEY
    Anatole Besarab; Fiscal Year: 1980
    ..and phosphate will be measured by clearance techniques and correlated with changes in cyclic 3'-5'-adenosine monophosphate (cyclic AMP) excretion under a variety of experimental conditions...
  50. REGULATORY ROLE OF CYCLIC NUCLEOTIDES IN MUCOR
    PETER BORGIA; Fiscal Year: 1980
    ..Cyclic adenosine monophosphate (cAMP) causes a reversible inactivation of beta-glucosidase...
  51. BRAIN GALANIN RECEPTORS AND EPILEPSY
    Andrey Mazarati; Fiscal Year: 2006
    ..Specifically, we will study how galanin receptor PNA treatment affects second messenger systems (cyclic adenosine monophosphate, phosphoinisitide turnover, mitogen-activated protein kinase activity, excitatory amino acid glutamate ..
  52. CYCLIC NUCLEOTIDE PHOSPHODIESTERASE IN ASTROGLIA
    VERGINE MADELIAN; Fiscal Year: 1992
    ..Cyclic nucleotide phosphodiesterases (PDE) are a group of enzymes that degrade 3',5'- cAMP to 5' adenosine monophosphate (AMP). One of the PDE types is selectively inhibited by RO 20-1724 and Rolipram...
  53. CATECHOLAMINE BIOSYNTHESIS IN THE OOCYTE
    Gary Smith; Fiscal Year: 2000
    ..Experiments have shown that this de novo synthesized NE is capable of regulating intrafollicular cyclic adenosine monophosphate (cAMP)...
  54. Pharmacogenetics Of Methotrexate In Rheumatoid Arthritis
    Laura Hughes; Fiscal Year: 2009
    ..to MTX by analyzing functional polymorphisms in genes encoding 2 key enzymes inhibited by MTX therapy;adenosine monophosphate deaminase-1 (AMPD1) and adenosine deaminase-1 (ADA1), in Caucasians and African-Americans with RA...
  55. PUTATIVE MEDIATORS OF INSULIN SECRETION
    SUZANNE LAYCHOCK; Fiscal Year: 2000
    ..include: heat shock proteins, stress-activated protein kinases (SAPKs), heme oxygenase induction, adenosine monophosphate-mediated protein kinase (AMPK) and acetyl-CoA carboxylase activity, and antioxidant enzyme induction/..
  56. PROLIFERATION & MALIGNANT TRANSFORMATION OF MELANOCYTES
    Ruth Halaban; Fiscal Year: 1992
    ....
  57. MOLECULAR MODE OF ACTION OF BACTERIAL ENTEROTOXINS
    JOHNNY PETERSON; Fiscal Year: 2001
    ..adenylate cyclase activity by catalyzing the ADP-ribosylation of Gs-alpha, thereby increasing 3',5'-adenosine monophosphate (cAMP) levels, equally important are stimulatory effects on arachidonic acid (AA) metabolism leading to ..
  58. ROLE OF C-AMP IN RENIN RELEASE
    WINFRED MAGEE; Fiscal Year: 1990
    ..release of renin by juxtaglomerular cells, and since adenosine is inhibitory, we propose that cyclic adenosine monophosphate serves as the intracellular mediator...
  59. CELL BIOLOGY OF RETINAL PIGMENT EPITHELIUM
    CYNTHIA HETH; Fiscal Year: 1993
    ..Changes in the concentration of three second messengers, cyclic adenosine monophosphate, inositol triphosphate and diacylglycerol, which may regulate the phagocytic mechanism in RPE, will be ..
  60. ROLE OF PGE2 RECEPTORS EP2 AND EP3 ON SENESCENCE
    Raymond Konger; Fiscal Year: 2004
    ..EP2 receptors is dependent on ligand- dependent activation of adenylate cyclase, with elevation of cyclic adenosine monophosphate (cAMP). In keratinocytes, EP3 receptors are coupled to a delayed increase in diacylglycerol (DAG)...
  61. SYNAPTIC PLASTICITY AND MEMORY CODING
    Howard Eichenbaum; Fiscal Year: 2003
    ..that inactivate isoforrns of calcium-calmoduline dependent kinase type 11 (CaMKII) and cyclic adenosine monophosphate (cAMP) responsive element binding protein (CREB), two molecules that are critical to the formation of ..
  62. Exploiting AMPK to Slow Polycystic Kidney Disease
    Vinita Takiar; Fiscal Year: 2009
    ..Both of these targets lie downstream of and are antagonized by Adenosine Monophosphate-activated Protein Kinase (AMPK)...
  63. Social Attachment as a Model of Addiction
    Adam Perry; Fiscal Year: 2006
    ..of oxytocin (OT) on the phosphorylation, and hence activity, of the transcription regulator cyclic adenosine monophosphate (cAMP) response element binding (CREB) protein in the medial amygdala (MeA), and the effect on social ..