adenosine diphosphate


Summary: Adenosine 5'-(trihydrogen diphosphate). An adenine nucleotide containing two phosphate groups esterified to the sugar moiety at the 5'-position.

Top Publications

  1. ncbi Analysis of mitochondrial function in situ in permeabilized muscle fibers, tissues and cells
    Andrey V Kuznetsov
    Daniel Swarovski Research Laboratory, Department of General and Transplant Surgery, Innsbruck Medical University, Innsbruck A6020, Austria
    Nat Protoc 3:965-76. 2008
  2. ncbi Consensus and future directions on the definition of high on-treatment platelet reactivity to adenosine diphosphate
    Laurent Bonello
    Department of Cardiology, Institut National de la Santè et de la Recherche Médicale Unité Mixte de Recherche 608, Hopital Universitaire Nord, Faculte de Medecine, Marseille, France
    J Am Coll Cardiol 56:919-33. 2010
  3. pmc A genetically encoded fluorescent reporter of ATP:ADP ratio
    Jim Berg
    Department of Neurobiology, Harvard Medical School, 220 Longwood Ave, Boston, Massachusetts 02115, USA
    Nat Methods 6:161-6. 2009
  4. ncbi Consensus and update on the definition of on-treatment platelet reactivity to adenosine diphosphate associated with ischemia and bleeding
    Udaya S Tantry
    Sinai Center for Thrombosis Research, Sinai Hospital of Baltimore, Baltimore, Maryland
    J Am Coll Cardiol 62:2261-73. 2013
  5. pmc Central role of the P2Y12 receptor in platelet activation
    Robert T Dorsam
    Department of Pharmacology, Temple University Medical School, Philadelphia, Pennsylvania 19140, USA
    J Clin Invest 113:340-5. 2004
  6. pmc Myosin I can act as a molecular force sensor
    Joseph M Laakso
    Pennsylvania Muscle Institute and Department of Physiology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
    Science 321:133-6. 2008
  7. ncbi Truncation of Kir6.2 produces ATP-sensitive K+ channels in the absence of the sulphonylurea receptor
    S J Tucker
    University Laboratory of Physiology, Oxford, UK
    Nature 387:179-83. 1997
  8. pmc Distantly related sequences in the alpha- and beta-subunits of ATP synthase, myosin, kinases and other ATP-requiring enzymes and a common nucleotide binding fold
    J E Walker
    EMBO J 1:945-51. 1982
  9. ncbi Identification of the platelet ADP receptor targeted by antithrombotic drugs
    G Hollopeter
    Department of Cellular and Molecular Pharmacology, University of California, San Francisco 94143, USA
    Nature 409:202-7. 2001
  10. ncbi Adenosine diphosphate as an intracellular regulator of insulin secretion
    C G Nichols
    Department of Cell Biology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Science 272:1785-7. 1996

Research Grants

Detail Information

Publications323 found, 100 shown here

  1. ncbi Analysis of mitochondrial function in situ in permeabilized muscle fibers, tissues and cells
    Andrey V Kuznetsov
    Daniel Swarovski Research Laboratory, Department of General and Transplant Surgery, Innsbruck Medical University, Innsbruck A6020, Austria
    Nat Protoc 3:965-76. 2008
    ..As only a small amount of tissue is required for analysis, the protocol can be used in diagnostic settings in clinical studies. The permeabilization procedure and specific titration analysis can be completed in 2 h...
  2. ncbi Consensus and future directions on the definition of high on-treatment platelet reactivity to adenosine diphosphate
    Laurent Bonello
    Department of Cardiology, Institut National de la Santè et de la Recherche Médicale Unité Mixte de Recherche 608, Hopital Universitaire Nord, Faculte de Medecine, Marseille, France
    J Am Coll Cardiol 56:919-33. 2010
    ..High on-treatment platelet reactivity to adenosine diphosphate (ADP) was observed in selected patients...
  3. pmc A genetically encoded fluorescent reporter of ATP:ADP ratio
    Jim Berg
    Department of Neurobiology, Harvard Medical School, 220 Longwood Ave, Boston, Massachusetts 02115, USA
    Nat Methods 6:161-6. 2009
    ..The engineered sensor, Perceval, can be used to monitor the ATP:ADP ratio during live-cell imaging...
  4. ncbi Consensus and update on the definition of on-treatment platelet reactivity to adenosine diphosphate associated with ischemia and bleeding
    Udaya S Tantry
    Sinai Center for Thrombosis Research, Sinai Hospital of Baltimore, Baltimore, Maryland
    J Am Coll Cardiol 62:2261-73. 2013
    ..In an earlier consensus document, we proposed cutoff values for high on-treatment platelet reactivity to adenosine diphosphate (ADP) associated with post-percutaneous coronary intervention ischemic events for various platelet ..
  5. pmc Central role of the P2Y12 receptor in platelet activation
    Robert T Dorsam
    Department of Pharmacology, Temple University Medical School, Philadelphia, Pennsylvania 19140, USA
    J Clin Invest 113:340-5. 2004
    ..The P2Y(12) receptor, activated by ADP, plays a central role in platelet activation and is the target of P2Y(12) receptor antagonists that have proven therapeutic value...
  6. pmc Myosin I can act as a molecular force sensor
    Joseph M Laakso
    Pennsylvania Muscle Institute and Department of Physiology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
    Science 321:133-6. 2008
    ..2) to a high (>0.9) duty-ratio motor. This impressive tension sensitivity supports a role for myosin I as a molecular force sensor...
  7. ncbi Truncation of Kir6.2 produces ATP-sensitive K+ channels in the absence of the sulphonylurea receptor
    S J Tucker
    University Laboratory of Physiology, Oxford, UK
    Nature 387:179-83. 1997
    ..We show here that the primary site at which ATP acts to mediate K-ATP channel inhibition is located on Kir6.2, and that SUR1 is required for sensitivity to sulphonylureas and diazoxide and for activation by Mg-ADP...
  8. pmc Distantly related sequences in the alpha- and beta-subunits of ATP synthase, myosin, kinases and other ATP-requiring enzymes and a common nucleotide binding fold
    J E Walker
    EMBO J 1:945-51. 1982
  9. ncbi Identification of the platelet ADP receptor targeted by antithrombotic drugs
    G Hollopeter
    Department of Cellular and Molecular Pharmacology, University of California, San Francisco 94143, USA
    Nature 409:202-7. 2001
    ..Cloning of the P2Y12 receptor should facilitate the development of better antiplatelet agents to treat cardiovascular diseases...
  10. ncbi Adenosine diphosphate as an intracellular regulator of insulin secretion
    C G Nichols
    Department of Cell Biology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Science 272:1785-7. 1996
    ..had normal ATP sensitivity, but unlike wild-type channels, inhibition by ATP was not antagonized by adenosine diphosphate (ADP)...
  11. ncbi Adenosine diphosphate-induced platelet aggregation is associated with P2Y12 gene sequence variations in healthy subjects
    Pierre Fontana
    Service d Hématologie Biologique A, Hôpital Européen Georges Pompidou and Inserm Unité 428, Faculte des Sciences Pharmaceutiques et Biologiques, Universite Paris V, Paris, France
    Circulation 108:989-95. 2003
    The adenosine diphosphate (ADP) receptor P2Y12 plays a pivotal role in platelet aggregation, as demonstrated by the benefit conferred by its blockade in patients with cardiovascular disease...
  12. pmc Microscopic evidence for a minus-end-directed power stroke in the kinesin motor ncd
    Thomas G Wendt
    European Molecular Biology Laboratory, Structure Programme, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
    EMBO J 21:5969-78. 2002
  13. ncbi Cloning of the beta cell high-affinity sulfonylurea receptor: a regulator of insulin secretion
    L Aguilar-Bryan
    Department of Cell Biology, Baylor College of Medicine, Houston, TX 77030, USA
    Science 268:423-6. 1995
    ..The results suggest that the sulfonylurea receptor may sense changes in ATP and ADP concentration, affect KATP channel activity, and thereby modulate insulin release...
  14. pmc PKCalpha regulates platelet granule secretion and thrombus formation in mice
    Olga Konopatskaya
    Department of Physiology and Pharmacology, School of Medical Sciences, University of Bristol, Bristol, United Kingdom
    J Clin Invest 119:399-407. 2009
    ..In summary, the effects of PKCalpha ablation on thrombus formation and granule secretion may implicate PKCalpha as a drug target for antithrombotic therapy...
  15. pmc Large conformational changes in a kinesin motor catalyzed by interaction with microtubules
    Keiko Hirose
    Gene Function Research Center, National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki 305 8562, Japan
    Mol Cell 23:913-23. 2006
  16. pmc Rotation of the stalk/neck and one head in a new crystal structure of the kinesin motor protein, Ncd
    Mikyung Yun
    Department of Structural Biology, St Jude Children s Research Hospital, Memphis, TN 38105, USA
    EMBO J 22:5382-9. 2003
    ..The rotation could represent a force-producing stroke that directs the motor to the minus end...
  17. pmc Myosin IC generates power over a range of loads via a new tension-sensing mechanism
    Michael J Greenberg
    The Pennsylvania Muscle Institute and Department of Physiology, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, PA 19104 6085, USA
    Proc Natl Acad Sci U S A 109:E2433-40. 2012
    ..Based on these results, we propose that myo1c functions as a slow transporter rather than a tension-sensitive anchor...
  18. pmc Actomyosin-ADP states, interhead cooperativity, and the force-velocity relation of skeletal muscle
    Alf Mansson
    School of Natural Sciences, Linnaeus University, Kalmar, Sweden
    Biophys J 98:1237-46. 2010
    ..This accords with the idea that attached myosin heads in the AM( *)ADP state position the partner heads for rapid attachment to the next site along actin, corresponding to the apparent increase in attachment rate in the model...
  19. pmc ADP ribosylation adapts an ER chaperone response to short-term fluctuations in unfolded protein load
    Joseph E Chambers
    Metabolic Research Laboratories, University of Cambridge, Cambridge CB2 0QQ, England, UK
    J Cell Biol 198:371-85. 2012
    ..searching for mechanisms that fill this temporal gap in coping with ER stress, we found elevated levels of adenosine diphosphate (ADP)-ribosylated BiP in the inactive pancreas of fasted mice and a rapid decline in this modification in ..
  20. ncbi Shaking the myosin family tree: biochemical kinetics defines four types of myosin motor
    Marieke J Bloemink
    School of Biosciences, University of Kent, Canterbury CT2 7NJ, UK
    Semin Cell Dev Biol 22:961-7. 2011
    ..Based on these properties we propose to classify myosins into four different groups: (I) fast movers, (II) slow/efficient force holders, (III) strain sensors and (IV) gates...
  21. ncbi Rac1 is essential for phospholipase C-gamma2 activation in platelets
    Irina Pleines
    Rudolf Virchow Center for Experimental Biomedicine, University of Wurzburg, Germany
    Pflugers Arch 457:1173-85. 2009
    ..Together, these results demonstrate that Rac1 is essential for ITAM-dependent PLCgamma2 activation in platelets and that this is critical for thrombus formation in vivo...
  22. ncbi Involvement of basic amino acid residues in transmembrane regions 6 and 7 in agonist and antagonist recognition of the human platelet P2Y(12)-receptor
    Kristina Hoffmann
    Department of Pharmacology, University of Bonn, Reuterstrasse 2b, D 53113 Bonn, Germany
    Biochem Pharmacol 76:1201-13. 2008
    ..Arg256 appears to play a role in the recognition of nucleotide agonists and the non-nucleotide antagonist reactive blue-2, but no role in the recognition of the nucleotide antagonist cangrelor...
  23. pmc Pause and rotation of F(1)-ATPase during catalysis
    Y Hirono-Hara
    Chemical Resources Laboratory, Tokyo Institute of Technology, 4259 Nagatsuta, Yokohama 226-8503, Japan
    Proc Natl Acad Sci U S A 98:13649-54. 2001
    ..The pausing position of the gamma subunit deviates from the ATP-waiting position and is most likely the recently found intermediate 90 degrees position...
  24. ncbi A ternary mechanism for NADH oxidation by positively charged electron acceptors, catalyzed at the flavin site in respiratory complex I
    James A Birrell
    Medical Research Council Mitochondrial Biology Unit, Wellcome Trust MRC Building, Cambridge, UK
    FEBS Lett 585:2318-22. 2011
    ..Our mechanism for paraquat reduction defines a new mechanism for superoxide production by complex I (by redox cycling); in contrast to direct O(2) reduction the rate is stimulated, not inhibited, by high NADH concentrations...
  25. ncbi Thiazolidinediones can rapidly activate AMP-activated protein kinase in mammalian tissues
    Nathan K Lebrasseur
    Diabetes and Metabolism Research Unit, Boston University School of Medicine, 650 Albany St, X 820, Boston, MA 02118, USA
    Am J Physiol Endocrinol Metab 291:E175-81. 2006
    ..They suggest that this effect is associated with a change in cellular energy state and that it is not dependent on PPARgamma-mediated gene transcription...
  26. pmc The macro domain is an ADP-ribose binding module
    Georgios I Karras
    Gene Expression Programme and Structural and Computational Biology Programme, European Molecular Biology Laboratory, Heidelberg, Germany
    EMBO J 24:1911-20. 2005
    ..In addition, some macro domain proteins also recognize poly-ADP-ribose as a ligand. Our data suggest an important role for proteins containing macro domains in the biology of ADP-ribose...
  27. ncbi Phosphate release in F1-ATPase catalytic cycle follows ADP release
    Rikiya Watanabe
    Institute of Scientific and Industrial Research, Osaka University, Osaka, Japan
    Nat Chem Biol 6:814-20. 2010
    ..These findings imply that under ATP synthesis conditions, P(i) binds to an empty catalytic site, preventing solution ATP (though not ADP) from binding. Thus, this supports the concept of selective ADP binding for efficient ATP synthesis...
  28. pmc A kinesin motor in a force-producing conformation
    Elisabeth Heuston
    Department of Chemistry, Dartmouth College, Hanover, NH 03755, USA
    BMC Struct Biol 10:19. 2010
    ..Perturbing the motor by introducing point mutations could stabilize transitional or unstable states, providing critical information about these rarer states...
  29. ncbi A model of myosin V processivity
    Steven S Rosenfeld
    Department of Neurology, University of Alabama, Birmingham, Alabama 35294, USA
    J Biol Chem 279:40100-11. 2004
  30. ncbi Interaction of the chaperone BiP with an antibody domain: implications for the chaperone cycle
    Gerhard Knarr
    Institut fur Organische Chemie und Biochemie, Technische Universitat Munchen, Lichtenbergstr 4, 85747 Garching, Germany
    J Mol Biol 318:611-20. 2002
    ..However, the accessibility of the BiP-binding site in the non-native protein seems to influence the kinetics of complex formation...
  31. ncbi Identification of P2Y12-dependent and -independent mechanisms of glycoprotein VI-mediated Rap1 activation in platelets
    Mark K Larson
    Department of Pharmacology, Center for Thrombosis and Hemostasis and Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, 27599, USA
    Blood 101:1409-15. 2003
    ..Interestingly, GPVI-mediated Rap1 activation in human platelets is largely dependent on adenosine diphosphate (ADP) signaling through the P2Y(12) and not the P2Y(1) receptor...
  32. ncbi G-protein-coupled receptors as signaling targets for antiplatelet therapy
    Susan S Smyth
    Veterans Affairs Medical Center, Department of Medicine, Physiology, and Pharmacology, University of Kentucky, Lexington, KY, USA
    Arterioscler Thromb Vasc Biol 29:449-57. 2009
    ..The identification and development of new agents directed against specific platelet signaling pathways may offer an advantage in preventing thrombotic events while minimizing bleeding risk...
  33. ncbi ATP induces proliferation of retinal cells in culture via activation of PKC and extracellular signal-regulated kinase cascade
    Glauco Sanches
    Departament of Neurobiology, Institute of Biology, Federal Fluminense University, Cx Postal 100180, Niteroi, RJ 24001 970, Brazil
    Int J Dev Neurosci 20:21-7. 2002
  34. ncbi Human keratinocytes release ATP and utilize three mechanisms for nucleotide interconversion at the cell surface
    Helen E Burrell
    Department of Human Anatomy and Cell Biology, School of Biomedical Sciences, University of Liverpool, The Sherrington Buildings, UK
    J Biol Chem 280:29667-76. 2005
    ..In this study we now identify the potential sources of extracellular ATP required to activate these receptors and provide better understanding of the role of nucleotides in normal epidermal homeostasis and wound healing...
  35. pmc DLP1-dependent mitochondrial fragmentation mediates 1-methyl-4-phenylpyridinium toxicity in neurons: implications for Parkinson's disease
    Xinglong Wang
    Department of Pathology, Case Western Reserve University, 2103 Connell Road, Cleveland, OH 44106, USA
    Aging Cell 10:807-23. 2011
    ..Overall, these findings suggest that DLP1-dependent mitochondrial fragmentation plays a crucial role in mediating MPP(+) -induced mitochondria abnormalities and cellular dysfunction and may represent a novel therapeutic target for PD...
  36. ncbi AMPK structure and regulation from three angles
    Bruce E Kemp
    Structure 15:1161-3. 2007
  37. pmc The biochemical kinetics underlying actin movement generated by one and many skeletal muscle myosin molecules
    Josh E Baker
    Department of Molecular Physiology and Biophysics, University of Vermont, Burlington, Vermont 05405, USA
    Biophys J 82:2134-47. 2002
  38. ncbi The mechanism of lithium action: state of the art, ten years later
    A Shaldubina
    Stanley Center for Bipolar Research, Ministry of Health Mental Health Center, Faculty of Health Sciences, Ben Gurion University of the Negev, Beer-Sheba, Israel
    Prog Neuropsychopharmacol Biol Psychiatry 25:855-66. 2001
    ..Biochemical and clinical experiments are necessary to determine the key mechanism of lithium efficacy in treatment and prophylaxis of affective disorders...
  39. pmc Structure of mammalian AMPK and its regulation by ADP
    Bing Xiao
    MRC National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, UK
    Nature 472:230-3. 2011
    ..From our biochemical and structural data we develop a model for how the energy status of a cell regulates AMPK activity...
  40. ncbi The brain uncoupling protein UCP4 attenuates mitochondrial toxin-induced cell death: role of extracellular signal-regulated kinases in bioenergetics adaptation and cell survival
    Zelan Wei
    Burnett School of Biomedical Sciences, College of Medicine, University of Central Florida, 4000 Central Florida Blvd, Orlando, FL 32816, USA
    Neurotox Res 16:14-29. 2009
    ..Collectively, our results support the involvement of ERKs in UCP4 dependent bioenergetics adaptation and cell survival...
  41. ncbi Dictyostelium myosin-IE is a fast molecular motor involved in phagocytosis
    Ulrike Dürrwang
    Abteilung Biophysik, Max Planck Institut fur medizinische Forschung, Jahnstr 29, 69120 Heidelberg, Germany
    J Cell Sci 119:550-8. 2006
    ..Changes in the level of free Mg(2+) ions, which are within the physiological range, are shown to modulate the motor activity of myosin-IE by inhibiting the release of adenosine diphosphate.
  42. ncbi Adenosine diphosphate (ADP)-induced thromboxane A(2) generation in human platelets requires coordinated signaling through integrin alpha(IIb)beta(3) and ADP receptors
    Jianguo Jin
    Department of Physiology, Temple University Medical School, Philadelphia, PA 19140, USA
    Blood 99:193-8. 2002
    b>Adenosine diphosphate (ADP) is a platelet agonist that causes platelet shape change and aggregation as well as generation of thromboxane A(2), another platelet agonist, through its effects on P2Y1, P2Y12, and P2X1 receptors...
  43. ncbi Plasma ectonucleotidases prevent desensitization of purinergic receptors in stored platelets: importance for platelet activity during thrombus formation
    Sandra Cauwenberghs
    Department of Biochemistry CARIM, the Sanquin Blood Bank South East, the Department of Health Risk Analysis and Toxicology NUTRIM, Maastricht University, Maastricht, The Netherlands
    Transfusion 46:1018-28. 2006
    ..In vivo, this is antagonized by ectonucleotidases on the surface of endothelial cells and white blood cells (WBCs). The receptor desensitization of ATP- and ADP-induced responses of PLTs stored in plasma without WBCs was investigated...
  44. pmc Processive translocation mechanism of the human Bloom's syndrome helicase along single-stranded DNA
    Máté Gyimesi
    Department of Biochemistry, Eotvos University, Pázmány P s 1 C, H 1117 Budapest, Hungary
    Nucleic Acids Res 38:4404-14. 2010
    ..The data also support the conclusion that BLM performs double-stranded DNA unwinding by fully active duplex destabilization...
  45. ncbi Bioenergetics at low oxygen: dependence of respiration and phosphorylation on oxygen and adenosine diphosphate supply
    E Gnaiger
    D Swarovski Research Laboratory, Department of Transplant Surgery, University Hospital Innsbruck, Anichstrasse 35, 6020, Innsbruck, Austria
    Respir Physiol 128:277-97. 2001
    ..oxygen affinities (1/P(50)) are reviewed with particular emphasis on the turnover effect under control of adenosine diphosphate ADP concentration, which increases the P(50) in active states...
  46. ncbi Structure and mechanism of the RuvB Holliday junction branch migration motor
    C D Putnam
    Department of Molecular Biology, Skaggs Institute for Chemical Biology, The Scripps Research Institute, MB 4, 10550 North Torrey Pines Rd, La Jolla, CA 92037, USA
    J Mol Biol 311:297-310. 2001
    ..Taken together, these results provide insights into how RuvB functions as a motor for branch migration of Holliday junctions...
  47. pmc Defective platelet aggregation and increased resistance to thrombosis in purinergic P2Y(1) receptor-null mice
    C Leon
    Institut National de la Santé et de la Recherche Médicale INSERM U 311, Etablissement de Transfusion Sanguine, 10, rue Spielmann, B P 36, 67065 Strasbourg, France
    J Clin Invest 104:1731-7. 1999
    ..Hence, the P2Y(1) receptor plays an essential role in thrombotic states and represents a potential target for antithrombotic drugs...
  48. pmc Resolvin E1 regulates adenosine diphosphate activation of human platelets
    Gabrielle Fredman
    Department of Anesthesiology, Perioperative, and Pain Medicine, Brigham and Women s Hospital and Harvard Medical School, Center for Experimental Therapeutics and Reperfusion Injury, Boston, Mass 02115, USA
    Arterioscler Thromb Vasc Biol 30:2005-13. 2010
    To investigate the ability of resolvin E1 (RvE1) to regulate adenosine diphosphate (ADP) activation of platelets via specific receptors because RvE1 reduces platelet aggregation with certain agonists, including ADP.
  49. ncbi Decreased platelet aggregation, increased bleeding time and resistance to thromboembolism in P2Y1-deficient mice
    J E Fabre
    Department of Medicine, University of North Carolina, CB 7248, Chapel Hill, Thurston Bowles Bld, Durham, North Carolina, USA
    Nat Med 5:1199-202. 1999
    ..These findings support the hypothesis that the ATP receptor P2Y1 is a principal receptor mediating both physiologic and pathological ADP-induced processes in platelets...
  50. ncbi Structure of bovine mitochondrial F(1)-ATPase with nucleotide bound to all three catalytic sites: implications for the mechanism of rotary catalysis
    R I Menz
    Medical Research Council Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, United Kingdom
    Cell 106:331-41. 2001
    ..A catalytic scheme for hydrolysis (and synthesis) at physiological rates and a mechanism for the ATP-driven rotation of the gamma subunit are proposed based on the crystal structures of the bovine enzyme...
  51. ncbi Vertebrate myosin VIIb is a high duty ratio motor adapted for generating and maintaining tension
    Arnon Henn
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06520 8114, USA
    J Biol Chem 280:39665-76. 2005
    ..Given the high conservation of vertebrate class VII myosins, deafness phenotypes arising from disruption of normal myosin VIIa function are likely to reflect a loss of tension in the stereocilia of inner ear hair cells...
  52. pmc Magnitude and control of mitochondrial sensitivity to ADP
    Jeroen A L Jeneson
    Biomedical NMR, Dept of Biomedical Engineering, Eindhoven University of Technology, 5600 MB Eindhoven, The Netherlands
    Am J Physiol Endocrinol Metab 297:E774-84. 2009
  53. ncbi Drosophila kinesin minimal motor domain expressed in Escherichia coli. Purification and kinetic characterization
    T G Huang
    Department of Biological Sciences Carnegie Mellon University, Pittsburgh, Pennsylvania 15213
    J Biol Chem 269:16493-501. 1994
  54. pmc CalDAG-GEFI and protein kinase C represent alternative pathways leading to activation of integrin alphaIIbbeta3 in platelets
    Stephen M Cifuni
    Immune Disease Institute, Harvard Medical School, Boston, MA, USA
    Blood 112:1696-703. 2008
    ..In summary, our study identifies CalDAG-GEFI and PKC as independent pathways leading to Rap1 and alphaIIbbeta3 activation in mouse platelets activated through the PAR4 receptor...
  55. pmc An ATP gate controls tubulin binding by the tethered head of kinesin-1
    Maria C Alonso
    Molecular Motors Group, Marie Curie Research Institute, The Chart, Oxted, Surrey RH8 0TL, UK
    Science 316:120-3. 2007
    ..We show that unpolymerized tubulin binds to kinesin-1, causing tubulin-activated release of adenosine diphosphate (ADP). With no added nucleotide, each kinesin-1 dimer binds one tubulin heterodimer...
  56. ncbi Hop modulates Hsp70/Hsp90 interactions in protein folding
    B D Johnson
    Department of Biochemistry and Molecular Biology, Mayo Graduate School, Rochester, Minnesota 55905, USA
    J Biol Chem 273:3679-86. 1998
    ..Conversely, once p23 is bound to hsp90, Hop binding is diminished. These results confirm that Hop provides a physical link between hsp70 and hsp90 and also indicate that Hop modulates the activities of both of these chaperone proteins...
  57. pmc Three conformational snapshots of the hepatitis C virus NS3 helicase reveal a ratchet translocation mechanism
    Meigang Gu
    Laboratory of Virology and Infectious Disease, Center for the Study of Hepatitis C, The Rockefeller University, New York, NY 10065, USA
    Proc Natl Acad Sci U S A 107:521-8. 2010
    ..These findings suggest feasible strategies for developing specific inhibitors to block the action of this attractive, yet largely unexplored drug target...
  58. pmc Structure of nucleotide-binding domain 1 of the cystic fibrosis transmembrane conductance regulator
    Hal A Lewis
    Structural GenomiX Inc, San Diego, CA, USA
    EMBO J 23:282-93. 2004
    ..Our results are consistent with a CFTR mechanism, whereby channel gating occurs through ATP binding in an NBD1-NBD2 nucleotide sandwich that forms upon displacement of NBD1 regulatory segments...
  59. ncbi The nucleotide receptor P2Y13 is a key regulator of hepatic high-density lipoprotein (HDL) endocytosis
    S Jacquet
    INSERM U563, Dpt Lipoprotéines et Médiateurs Lipidiques, Bat C, Hopital Purpan, 31024 Toulouse, France
    Cell Mol Life Sci 62:2508-15. 2005
    ..We also found a new important action of the antithrombotic agent AR-C69931MX as a strong activator of P2Y13-mediated HDL endocytosis...
  60. ncbi Coupling of rotation and catalysis in F(1)-ATPase revealed by single-molecule imaging and manipulation
    Kengo Adachi
    Department of Physics, Faculty of Science and Engineering, Waseda University, Shinjuku ku, Tokyo 169 8555, Japan
    Cell 130:309-21. 2007
    ..This and other results suggest that the affinity for ADP also decreases with rotation, and thus ADP release contributes part of energy for rotation. Together with previous results, the coupling scheme is now basically complete...
  61. pmc Direct visualization of asymmetric adenine-nucleotide-induced conformational changes in MutL alpha
    Elizabeth J Sacho
    Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    Mol Cell 29:112-21. 2008
    ..These data reveal an ATPase cycle in which sequential nucleotide binding, hydrolysis, and release modulate the conformational states of MutL alpha...
  62. ncbi Mechanism of blebbistatin inhibition of myosin II
    Mihaly Kovacs
    Laboratory of Molecular Cardiology, NHLBI, National Institutes of Health, Bethesda, Maryland 20892 1762, USA
    J Biol Chem 279:35557-63. 2004
  63. ncbi Kinetic mechanism and regulation of myosin VI
    E M De La Cruz
    Department of Physiology, Pennsylvania Muscle Institute, University of Pennsylvania School of Medicine, 3700 Hamilton Walk, Philadelphia, Pennsylvania 19104-6085, USA
    J Biol Chem 276:32373-81. 2001
    ..We propose an alternating site model for the stepping and processivity of two-headed high duty ratio myosins...
  64. ncbi Which platelet function test is suitable to monitor clopidogrel responsiveness? A pharmacokinetic analysis on the active metabolite of clopidogrel
    H J Bouman
    Department of Cardiology, St Antonius Hospital, Nieuwegein, The Netherlands
    J Thromb Haemost 8:482-8. 2010
    ..Multiple platelet function tests claim to be P2Y12-pathway specific and capable of capturing the biological activity of clopidogrel...
  65. pmc Protein kinase Ctheta negatively regulates store-independent Ca2+ entry and phosphatidylserine exposure downstream of glycoprotein VI in platelets
    Matthew T Harper
    Department of Physiology and Pharmacology, School of Medical Sciences, University of Bristol, BS8 1TD Bristol, United Kingdom
    J Biol Chem 285:19865-73. 2010
    ..We propose that PKCtheta normally acts to restrict store-independent Ca(2+) entry during GPVI signaling, which results in reduced PS exposure, limiting platelet procoagulant activity during thrombus formation...
  66. pmc Replication cycle-coordinated change of the adenine nucleotide-bound forms of DnaA protein in Escherichia coli
    K Kurokawa
    Department of Molecular Microbiology, Kyushu University Graduate School of Pharmaceutical Sciences, 3 1 1 Maidashi, Higashi ku, Fukuoka, 812 8582, Japan
    EMBO J 18:6642-52. 1999
    ..Regeneration of ATP-DnaA from ADP-DnaA was also observed. These results indicate that the nucleotide form shifts of DnaA are tightly linked with an epistatic cell cycle event and with the chromosomal replication system...
  67. ncbi Clopidogrel and ticlopidine: P2Y12 adenosine diphosphate-receptor antagonists for the prevention of atherothrombosis
    Pierre Savi
    Cardiovascular Thrombosis Research Department, Sanofi Aventis Recherche, 195 route d Espagne, 31036 Toulouse Cedex, France
    Semin Thromb Hemost 31:174-83. 2005
    Ticlopidine and clopidogrel belong to the same chemical family of thienopyridine adenosine diphosphate (ADP)-receptor antagonists...
  68. ncbi Sodium-induced GCN4 expression controls the accumulation of the 5' to 3' RNA degradation inhibitor, 3'-phosphoadenosine 5'-phosphate
    Anne Laure Todeschini
    Institut de Biologie Physico Chimique, Paris, France
    J Biol Chem 281:3276-82. 2006
  69. pmc Load-dependent mechanism of nonmuscle myosin 2
    Mihaly Kovacs
    Laboratory of Molecular Physiology, National Heart, Lung, and Blood Institute NIH, Bethesda, MD 20892 8015, USA
    Proc Natl Acad Sci U S A 104:9994-9. 2007
    ..Whereas forward load accelerates the cycle of interaction with actin, resistive load increases duty ratio to favor tension maintenance by two-headed attachment...
  70. ncbi The human mismatch recognition complex hMSH2-hMSH6 functions as a novel molecular switch
    S Gradia
    Department of Microbiology and Immunology, Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
    Cell 91:995-1005. 1997
    ..These results suggest a new model for the function of MutS proteins during mismatch repair in which the switch determines the timing of downstream events...
  71. ncbi Overexpression of the platelet P2X1 ion channel in transgenic mice generates a novel prothrombotic phenotype
    Cecile Oury
    Center for Molecular and Vascular Biology, University of Leuven, Belgium
    Blood 101:3969-76. 2003
    ..In contrast, transgenic platelet responses to adenosine diphosphate (ADP) or thrombin were normal...
  72. ncbi Regulation of the HscA ATPase reaction cycle by the co-chaperone HscB and the iron-sulfur cluster assembly protein IscU
    Jonathan J Silberg
    Department of Physiology and Biophysics, University of California, Irvine, California 92697, USA
    J Biol Chem 279:53924-31. 2004
    ..ATP T-state is regenerated. Because HscA nucleotide exchange is fast, the overall rate of the chaperone cycle in vivo will be determined by the availability of the IscU-HscB substrate-co-chaperone complex...
  73. ncbi ATP excites interneurons and astrocytes to increase synaptic inhibition in neuronal networks
    David N Bowser
    Medical Research Council Laboratory of Molecular Biology, Cambridge CB2 2QH, United Kingdom
    J Neurosci 24:8606-20. 2004
    ..Overall, our data are consistent with the hypothesis that ATP released from neurons and astrocytes acts on P2Y1 receptors to excite interneurons, resulting in increased synaptic inhibition within intact hippocampal circuits...
  74. ncbi P2Y1 receptor signaling enhances neuroprotection by astrocytes against oxidative stress via IL-6 release in hippocampal cultures
    Takumi Fujita
    Department of Molecular and System Pharmacology, Graduate School of Pharmaceutical Sciences, Kyushu University, Higashi ku, Fukuoka, Japan
    Glia 57:244-57. 2009
    ..Thus, activation of P2Y(1)R in astrocytes may rescue neurons from secondary cell death under pathological conditions...
  75. ncbi A membrane network of receptors and enzymes for adenine nucleotides and nucleosides
    Klaus Schicker
    Institute of Pharmacology, Medical University of Vienna, Waehringerstrasse 13a, A 1090 Vienna, Austria
    Biochim Biophys Acta 1793:325-34. 2009
    ..Thus, purinergic signalling is not only determined by the expression of receptors and enzymes but also by their direct interaction within a previously unrecognized multifarious membrane network...
  76. pmc Prolonged nonhydrolytic interaction of nucleotide with CFTR's NH2-terminal nucleotide binding domain and its role in channel gating
    Claudia Basso
    Laboratory of Cardiac Membrane Physiology, The Rockefeller University, 1230 York Ave, New York, NY 10021, USA
    J Gen Physiol 122:333-48. 2003
  77. pmc Two neonatal diabetes mutations on transmembrane helix 15 of SUR1 increase affinity for ATP and ADP at nucleotide binding domain 2
    David Ortiz
    Graduate Program in Structural and Computational Biology and Molecular Biophysics, Baylor College of Medicine, Houston, Texas 77030, USA
    J Biol Chem 287:17985-95. 2012
    ..NBD2, but not NBD1, has a higher affinity for ATP (and ADP) in mutant versus wild type (with or without Mg(2+)). Thus, the mutants spend more time in nucleotide-bound conformations, with reduced affinity for GBC, that activate the pore...
  78. pmc Switch II mutants reveal coupling between the nucleotide- and actin-binding regions in myosin V
    Darshan V Trivedi
    Department of Cellular and Molecular Physiology, College of Medicine, Pennsylvania State University, Hershey, Pennsylvania, USA
    Biophys J 102:2545-55. 2012
    ..Our results indicate that the switch II region is critical for stabilizing the closed NBP conformation in the presence of actin, and is essential for communication between the active site and actin-binding region...
  79. pmc Lithium toxicity in yeast is due to the inhibition of RNA processing enzymes
    B Dichtl
    EMBL, Gene Expression Programme, Postfach 10 2209, 69012 Heidelberg, Germany
    EMBO J 16:7184-95. 1997
    ..We propose that Li+ toxicity in yeast is due to synthetic lethality evoked between Xrn1p and RNase MRP. Similar mechanisms may contribute to the effects of Li+ on development and in human neurobiology...
  80. pmc Rhythmic ring-ring stacking drives the circadian oscillator clockwise
    Yong Gang Chang
    School of Natural Sciences, University of California, Merced, CA 95343, USA
    Proc Natl Acad Sci U S A 109:16847-51. 2012
    ..We demonstrate that ADP can expose the KaiB-binding site in the absence of ring stacking, providing an explanation for how it can reset the clock...
  81. pmc The ecto-nucleotidase NTPDase1 differentially regulates P2Y1 and P2Y2 receptor-dependent vasorelaxation
    Gilles Kauffenstein
    Centre de Recherche en Rhumatologie et Immunologie, Centre Hospitalier Universitaire de Quebec, Universite Laval, Quebec, QC, Canada
    Br J Pharmacol 159:576-85. 2010
    ..As these autacoids are actively metabolized by the ecto-nucleotidase nucleoside triphosphate diphosphohydrolase-1 (NTPDase1), we studied the effects of this cell surface enzyme on nucleotide-dependent vasodilatation...
  82. ncbi The ADP and ATP transport in mitochondria and its carrier
    Martin Klingenberg
    Institut Physiologische Chemie der Universität München, Germany
    Biochim Biophys Acta 1778:1978-2021. 2008
    ..Being a snapshot of an extreme abortive "c-state" the actual mechanism still remains a conjecture...
  83. ncbi Preferential substrate binding orientation by the molecular chaperone HscA
    Tim L Tapley
    Department of Physiology and Biophysics, University of California Irvine, Irvine, CA 92697, USA
    J Biol Chem 279:28435-42. 2004
  84. ncbi Crystal structure of human sulfotransferase SULT1A3 in complex with dopamine and 3'-phosphoadenosine 5'-phosphate
    Jing Hua Lu
    National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Chaoyang District, Bejing 100101, PR China
    Biochem Biophys Res Commun 335:417-23. 2005
    ..On the other hand, residue Asp86 appeared to be critical to the Mn2+-stimulation of the Dopa/tyrosine-sulfating activity of SULT1A3, in addition to a supporting role in the stereoselectivity and sulfating activity...
  85. ncbi Modified diadenosine tetraphosphates with dual specificity for P2Y1 and P2Y12 are potent antagonists of ADP-induced platelet activation
    H Chang
    Department of Pediatrics, University of Massachusetts Medical School, Worcester, MA, USA
    J Thromb Haemost 10:2573-80. 2012
    ..Modification of the Ap(4)A tetraphosphate backbone enhances inhibition of ADP-induced platelet aggregation. However, the effects of these Ap(4)A analogs on human platelet P2Y(1), P2Y(12) and P2X1 are unclear...
  86. ncbi Adenine nucleotide regulation in pancreatic beta-cells: modeling of ATP/ADP-Ca2+ interactions
    Leonid E Fridlyand
    Dept of Medicine, The Univ of Chicago, 5841 S Maryland Ave, Chicago, IL 60637, USA
    Am J Physiol Endocrinol Metab 289:E839-48. 2005
    ..The results demonstrate the utility of comprehensive mathematical modeling in understanding the ramifications of potential defects in beta-cell function in diabetes...
  87. pmc Glibenclamide binding to sulphonylurea receptor subtypes: dependence on adenine nucleotides
    Annette Hambrock
    Department of Pharmacology, Medical Faculty, University of Tubingen, Wilhelmstr 56, D 72074 Tubingen, Germany
    Br J Pharmacol 136:995-1004. 2002
    ..2/SUR1. 6: The data show (a) that the inhibitory effects of ATP and ADP on glibenclamide binding differ from one another, (b) that they depend on the SUR subtype, and (c) that they are weakened by coexpression with Kir6.2...
  88. pmc Two discriminatory binding sites in the Escherichia coli replication origin are required for DNA strand opening by initiator DnaA-ATP
    Kevin C McGarry
    Department of Biological Sciences, Florida Institute of Technology, 150 West University Boulevard, Melbourne, FL 32901, USA
    Proc Natl Acad Sci U S A 101:2811-6. 2004
    ..These observations indicate that specific initiator binding sites within a replication origin can be important determinants of an ATP-dependent molecular switch regulating DNA strand separation...
  89. pmc ATP binding to the motor domain from an ABC transporter drives formation of a nucleotide sandwich dimer
    Paul C Smith
    Department of Biological Sciences, 702A Fairchild Center, MC2434, Columbia University, New York, New York 10027, USA
    Mol Cell 10:139-49. 2002
    ..These results establish the stereochemical basis of the power stroke of ABC transporter pumps...
  90. pmc Dph3, a small protein required for diphthamide biosynthesis, is essential in mouse development
    Shihui Liu
    Bacterial Toxins and Therapeutics Section, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
    Mol Cell Biol 26:3835-41. 2006
    ..These results attest to the physiological importance of Dph3 in development. The biological roles of Dph3 are also discussed...
  91. pmc Evidence for a SAL1-PAP chloroplast retrograde pathway that functions in drought and high light signaling in Arabidopsis
    Gonzalo M Estavillo
    Australian Research Council Centre of Excellence in Plant Energy Biology, Research School of Biology, Australian National University Canberra, Acton, Australian Capital Territory 0200, Australia
    Plant Cell 23:3992-4012. 2011
    ..We propose a SAL1-PAP retrograde pathway that can alter nuclear gene expression during HL and drought stress...
  92. ncbi Extracellular nucleotide signaling in adult neural stem cells: synergism with growth factor-mediated cellular proliferation
    Santosh K Mishra
    Biocenter, J W Goethe University, AK Neurochemistry, Frankfurt am Main, Germany
    Development 133:675-84. 2006
    ..Together with the in situ data, this supports the notion that extracellular nucleotides contribute to the control of adult neurogenesis...
  93. ncbi The roles of ADP and TXA in botrocetin/VWF-induced aggregation of washed platelets
    J Liu
    Division of Experimental Hematology, St Jude Children s Research Hospital, Memphis, TN 38152, USA
    J Thromb Haemost 2:2213-22. 2004
    ..The roles of ADP and thromboxane A2 (TXA2) in agglutination-induced GPIbalpha-mediated platelet activation have not been fully described...
  94. ncbi Use of acetone to attain highly active and soluble DNA packaging protein Gp16 of Phi29 for ATPase assay
    Lisa P Huang
    Department of Pathobiology, Purdue University, West Lafayette, IN 47907, USA
    Virology 312:449-57. 2003
    ..However, mutation on amino acids G(248) and D(256) did not affect the function of gp16 in DNA packaging...
  95. ncbi Evolutionarily conserved mammalian adenine nucleotide translocase 4 is essential for spermatogenesis
    Jeffrey V Brower
    Department of Pathology, University of Florida College of Medicine, Gainesville, Florida 32610, USA
    J Biol Chem 282:29658-66. 2007
  96. ncbi Structural model for strain-dependent microtubule activation of Mg-ADP release from kinesin
    Ryo Nitta
    Department of Cell Biology and Anatomy, University of Tokyo, Graduate School of Medicine, 7 3 1 Hongo, Bunkyo ku, Tokyo, 113 0033, Japan
    Nat Struct Mol Biol 15:1067-75. 2008
    ..We further propose that this set of events is linked to a strain-dependent docking of the neck linker to the motor core, which produces a two-step power stroke...
  97. ncbi The crystal structure of an ADP complex of Bacillus subtilis pyridoxal kinase provides evidence for the parallel emergence of enzyme activity during evolution
    Joseph A Newman
    Krebs Institute for Biomolecular Research, Department of Molecular Biology and Biotechnology, The University of Sheffield, Sheffield S10 2TN, UK
    J Mol Biol 363:520-30. 2006
    ..Moreover this further suggests that this enzyme activity has evolved independently on multiple occasions from within the ribokinase superfamily...
  98. ncbi Relative contribution of G-protein-coupled pathways to protease-activated receptor-mediated Akt phosphorylation in platelets
    Soochong Kim
    Department of Physiology, Temple University School of Medicine, Philadelphia, PA 19140, USA
    Blood 107:947-54. 2006
    ..G(12/13) activation might mediate its potentiating effect through Src activation, and Src kinases play an important role in thrombin-mediated Akt phosphorylation...
  99. pmc Electrical stimulation of the mucosa evokes slow EPSPs mediated by NK1 tachykinin receptors and by P2Y1 purinoceptors in different myenteric neurons
    Rachel M Gwynne
    Department of Physiology, University of Melbourne, Parkville, Victoria 3010, Australia
    Am J Physiol Gastrointest Liver Physiol 297:G179-86. 2009
    ..Thus activation of pathways running from the mucosa excites three different types of slow EPSP in myenteric neurons, which are mediated by either a tachykinin (NK1, AH neurons) or a purine nucleotide (P2Y(1), S neurons)...
  100. ncbi Properties of the kinesin-3 NcKin3 motor domain and implications for neck function
    Sarah Adio
    Department of Physical Biochemistry, Max Planck Institute for Biophysical Chemistry, Gottingen, Germany
    FEBS J 276:3641-55. 2009
    ..This is the first direct comparison of a monomeric Kinesin-3 with its dimeric full-length counterpart, and the kinetic changes observed here may also apply to other Kinesin-3 motors...
  101. ncbi A new role for the A2b adenosine receptor in regulating platelet function
    D Yang
    Department of Biochemistry, Boston University School of Medicine, Boston, MA 02118, USA
    J Thromb Haemost 8:817-27. 2010
    ..The A2a adenosine receptor has already been recognized as a mediator of adenosine-dependent effects on platelet aggregation, and here we present a new role for the A2b adenosine receptor (A2bAR) in this process...

Research Grants62

  1. Nitric Oxide--Neurotoxic Mechanisms of MPTP
    Allen Mandir; Fiscal Year: 2002
    ..Upon activation, the enzyme hydrolyzes NAD to nicotinamide and transfers adenosine diphosphate (ADP) ribose units to a variety of nuclear proteins including histones and PARP-1 itself...
  2. Local Calcium Signaling in Pulmonary Arteries
    JAMES SK SHAM; Fiscal Year: 2012
    ..RyRs are modulated by multiple mechanisms. Cyclic adenosine diphosphate-ribose (cADPR) is a major endogenous messenger for the RyR-gated Ca2+ release...
  3. Pathogenesis of Oxidative Stress in Chagas Disease
    Nisha Jain Garg; Fiscal Year: 2013
    ..LV function during the progression of Chagas disease, and (3) ROS induced DNA oxidative adducts and poly(adenosine diphosphate ribose) polymerase 1 (PARP-1) activation signaled inflammatory cytokine response in infected ..
  4. Chemical approaches for studying the biology of CD38
    Hening Lin; Fiscal Year: 2013
    ..The enzymatic activity can convert nicotinamide adenine dinucleotide (NAD) to adenosine diphosphate ribose (ADPR) and cyclic ADPR (cADPR), and NAD phosphate (NADP) to nicotinic acid adenine dinucleotide ..
    Sufan Chien; Fiscal Year: 2013
    ..which oxygen delivery to the body is inadequate for the generation of the adenosine triphosphate (ATP) and adenosine diphosphate (ADP) necessary to maintain the function and structural integrity of tissues...
  6. Diphthamide biosynthesis
    Hening Lin; Fiscal Year: 2012
    ..These toxins catalyze the transfer of an adenosine diphosphate ribose (ADP-ribose) from nicotinamide adenine dinucleotide (NAD) to one of the nitrogen atoms in the ..
  7. Purinergic G protein signal integration in nociceptors
    Derek C Molliver; Fiscal Year: 2013 peripheral sensory neurons conveying pain and is mediated by G protein-coupled receptors (GPCRs) for adenosine diphosphate (ADP)...
  8. Regulation of Pulmonary Inflammation by Leukotriene E4
    Joshua A Boyce; Fiscal Year: 2013
    ..P2Y12 receptors mediate a convergent pathway by which adenosine diphosphate (ADP) and leukotriene (LT)E4, respectively, facilitate the migration of effector cells to the allergen-..
  9. Molecular Modulation of Catalytic Sites of Escherichia coli F1Fo ATP Synthase
    Zulfiqar Ahmad; Fiscal Year: 2009 animals, plants, and almost all microorganisms in the form of adenosine triphosphate (ATP) from adenosine diphosphate (ADP) and inorganic phosphate (Pi)...
  10. Role and Mechanism of ATP Hydrolysis in Actin
    Isaiah Sumner; Fiscal Year: 2012
    ..Actin binds to adenosine triphosphate (ATP) and will also catalyze its hydrolysis to adenosine diphosphate (ADP)...
    Mone Zaidi; Fiscal Year: 2003
    ..They are gated primarily by Ca2+ and the second messenger, cyclic adenosine diphosphate-ribose (cADPr)...
    Thomas Bell; Fiscal Year: 1991
    ..Aggregation of BHT platelets does not occur in response to adenosine diphosphate (ADP), platelet activating factor (PAF), or A23187; is reversible in response to epinephrine; and complete ..
    Peter Becker; Fiscal Year: 2001
    ..Finally, we will study the response in whole eggs to photolysis of caged cADPr and caged Ca2+ t explore the spatial and kinetic relation between the Ca2+ release triggered by these two agents. ..
    Mark Francis; Fiscal Year: 2002
    ..Using NAD as a source, bacterial toxins add adenosine diphosphate ribose (ADP-ribose) to proteins, a process called ADP-ribosylation...
    Steven Graham; Fiscal Year: 2001
    ..Cyclic adenosine diphosphate ribose (cADPR) is one of a family of small molecule phosphates known to cause calcium release, and recent ..
    Robert Post; Fiscal Year: 1990
    ..The reactivity of the radioactive phosphoenzyme will be assessed by its response to adenosine diphosphate or potassium chloride added during a chase of unlabeled adenosine triphosphate...
    GEORGE ROUSH; Fiscal Year: 1993
    b>Adenosine diphosphate ribosyl transferase (ADPRT) and unscheduled DNA synthesis (UDS) are inter-related measures that may be obtained in mononuclear leukocytes from venous blood...
    ROBERT MALECZKA; Fiscal Year: 2001
    ..Interestingly, while the phomactins inhibit PAF-induced platelet aggregation, no effect on adenosine diphosphate, arachidonic acid, and collagen-induced platelet aggregation has been observed...
    Michael Steer; Fiscal Year: 1980
    ..Structural changes which accompany adenosine diphosphate and/or catecholamine hormone-induced shape change will be evaluated using techniques of fluorescence and ..
  20. Synthesis of Biologically Active Macrocycle Molecules
    ROBERT MALECZKA; Fiscal Year: 2006
    ..The phomactins are uniquely selective PAF antagonists as they have no effect on adenosine diphosphate, arachidonic acid, or collagen-induced platelet aggregation...
    FREDERICK TOBACK; Fiscal Year: 1993
    ..kidney epithelial cells (BSC-1 line) permitted us to identify other growth-stimulating agents such as adenosine diphosphate (ADP)...
  22. Genome-Wide Association of Platelet Phenotypes
    Lewis Becker; Fiscal Year: 2009
    ..Phenotypes of highest priority include aggregation induced by collagen, adenosine diphosphate (ADP), arachidonic acid (AA), and epinephrine in platelet rich plasma (PRP), ATP release induced by AA, ..
  23. Generic Fluorescent HTS Assay for Protein Kinases
    Robert Lowery; Fiscal Year: 2005
    ..The assay relies on a competitive fluorescence polarization-based immunoassay for detection of adenosine diphosphate (ADP), a product of all kinase reactions...
  24. Mechanisms of Pro-Thrombosis in Diabetes Mellitus
    David Schneider; Fiscal Year: 2003
    ..Further, these results will define the importance of prothrombotic potential in diabetic subjects while potentially identifying new therapeutic targets in patients with diabetes and other insulin resistant states. ..
    WILLIAM MAYHAN; Fiscal Year: 2006
    ..In addition, results of these studies may provide insights regarding possible therapeutic approaches for the treatment of nicotine-induced vascular dysfunction. ..
    David Hackney; Fiscal Year: 2002
    ..These enzymatic studies will be coupled with study of the motility that kinesin is able to produce. A long range goal is to be able to model the physical properties of kinesin based on its solution biochemical mechanism, ..
  27. Cerebrovascular Disease in Type 1 Diabetes
    WILLIAM MAYHAN; Fiscal Year: 2007
    ..We suggest that our results will provide novel insights regarding therapeutic approaches for the treatment/prevention of cerebrovascular dysfunction in diabetes. ..
  28. Cerebral Microcirculation During Alcohol Consumption
    WILLIAM MAYHAN; Fiscal Year: 2008
    ..These studies will provide valuable new insights and will lead to new therapeutic approaches for the treatment of cerebrovascular abnormalities observed in chronic alcoholics. [unreadable] [unreadable]..
  29. The mechanism of immunosuppression by thymosin beta 4.
    ELENA YARMOLA; Fiscal Year: 2007
    ..As an initial step toward these goals, I specifically propose to investigate the mechanism by which thymosin beta4 is oxidized, secreted, and recognized by cells of the immune system. ..
  30. Biomolecular Analysis of Proteins in Visual Disease
    ESTHER BISWAS FISS; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
    Aaron Marcus; Fiscal Year: 2003
  32. Iron Therapy in Renal Disease: Potential Toxicities
    RICHARD ZAGER; Fiscal Year: 2008
    ..Potential reasons for these differences will be assessed, with the ultimate goal of defining the safest way of administering parenteral irons to renal disease patients. ..
  33. Confocal Equipment for Biomedical and Nanomedicine Research
    Tzyh Chang Hwang; Fiscal Year: 2006
    ..unreadable] [unreadable] [unreadable]..
  34. Gating of CFTR CI Channels by ATP Hydrolysis
    Tzyh Chang Hwang; Fiscal Year: 2009
    ..A clear understanding of the molecular mechanisms of CFTR function will aid in designs of therapeutical reagents for the treatment of cystic fibrosis, secretory diarrhea, and other CFTR-associated diseases. ..
  35. Mechanisms of Nucleotide Release by Airway Epithelia
    Eduardo Lazarowski; Fiscal Year: 2006
    ..Delineation of nucleotide-releasing mechanisms will provide new possibilities to improve MCC function in chronic lung diseases characterized by mucin-obstructed and inflamed airways. [unreadable] [unreadable]..
    RICHARD ZAGER; Fiscal Year: 2001
    Aaron Marcus; Fiscal Year: 2005
    ..This collaboration, based on compelling feasibility data and historical collaborative success, will advance the understanding of CD39 thromboregulation, and lead to a novel therapeutic agent for thrombotic diathesis. ..
    DREW CARLSON; Fiscal Year: 2005
    ..The mechanisms revealed are likely to play a significant role in the suppression of host defense and the onset of septic shock...
    Subhasis Biswas; Fiscal Year: 2002
    ..Biochemical studies of purified helicase A will characterize the nucleotidase and helicase activities of this protein. ..
    Thomas Smith; Fiscal Year: 2004
    ..These studies have the potential of changing the way we view the early steps in the viral infection process and will allow us to develop tools that are applicable to other viral systems. ..
  41. MALDI-TOF MS for High Throughput SNP Analysis
    ALAN SHULDINER; Fiscal Year: 2002
    ..The technology will be of great benefit to a large number of NIH-funded projects and researchers, opportunities and will greatly accelerate the pace of discovery in this post-genomic era. ..
  42. PARS and peroxynitrite induced cell death
    Csaba Szabo; Fiscal Year: 2009
    ..4 To establish the role of Bel family proteins in the regulation of calcium homeostasis during peroxynitrite-induced cell death. The studies proposed here have broad pathophysiological relevance. ..
    Britton Chance; Fiscal Year: 2002
    ..If ourintended goals of demonstrating feasibility are achieved, then application for support through regular RO1channels would appear to be justified. ..
    Ludmila Belayev; Fiscal Year: 2003
    ..The current project will enable the applicants to move forward in two years, near to the level of the beginning of Phase II efficacy trial in septic patients. PROPOSED COMMERCIAL APPLICATION: Not Available ..
  45. Mitochondrial Dysfunction in Aged Muscle
    David J Marcinek; Fiscal Year: 2010
    ..2) Our long-term goal is the development of non- invasive methods to diagnose mitochondrial dysfunction and follow the progress of interventions meant to reverse disability in the elderly. ..
  46. CCD Camera for Intermediate Voltage Electron Microscopes
    Kenneth H Downing; Fiscal Year: 2010
    ..These adaptations will produce substantial benefits in work ranging from high-resolution structural studies of proteins and viruses to the three dimensional study of cell ultrastructure. ..
  47. Brain K+ Dynamics, BBB Breakdown & Hemorrhagic Transformation in Ischemic Stroke
    Stephen Jones; Fiscal Year: 2009
    ..This proposal's investigation of the relation of BBB breakdown to HT could lead directly to additional di- agnostic and therapeutic advances in the assessment and management of acute ischemic stroke. ..
  48. Ultrapotent PARS inhibitor for CNS trauma
    Csaba Szabo; Fiscal Year: 2003
    ..Estimated worldwide revenues after market entry and maturation (ca. 4 years after FDA approval)=$750 million (annual). ..
  49. Identification of Metabolic Virulence Factors in the Epidemic Typhus Rickettsia
    JONATHON AUDIA; Fiscal Year: 2008
    ..prowazekii and are, thus, potential targets for the development of novel antimicrobials to treat this obligate intracytoplasmic pathogen. [unreadable] [unreadable] [unreadable] [unreadable]..
  50. Retinal Oxygenation in Diabetic Retinopathy
    BRUCE BERKOWITZ; Fiscal Year: 2004
    ..The results of these studies could lead to the development of a non-invasive real time method for the early evaluation of diabetic retinopathy and its treatment. ..
    ALAN SHULDINER; Fiscal Year: 2004
    ..These advances will impact substantially on the quality of life of millions of older Americans with osteoporosis. ..
  52. Role of Natriuretic Peptides in the Cochlea
    George Trachte; Fiscal Year: 2004
    ..abstract_text> ..
  53. Myicardial injury: role of poly (ADP-ribose) synthetase
    Csaba Szabo; Fiscal Year: 2005
    ..Further, based on exciting preliminary data, the final, fifth aim is focusing in establishing the role of PARS in myocardial preconditioning. ..
    Satadal Chatterjee; Fiscal Year: 2005
    Andras Perl; Fiscal Year: 2004
    ..The proposed studies with the availability of unique reagents and existing model systems will further our knowledge on the role of TAL in the PPP, apoptosis signaling, and related disease processes. ..
  56. Bioanalytical Applications of Capillary Electrophoresis
    FRANK GOMEZ; Fiscal Year: 2005
    ..abstract_text> ..
    Michael Kotlikoff; Fiscal Year: 2004
    ..abstract_text> ..
  58. Heterogeneity of hypoxic Ca2+ release in pulmonary and *
    Yong Xiao Wang; Fiscal Year: 2006
    ..The proposed studies are also of fundamental physiological significance with respect to the regulation of Ca2+ release in other cell types. ..
  59. 2006 Biophysical Discussions - Molecuar Motors: Point Counterpoint
    Steven Rosenfeld; Fiscal Year: 2006
    ..unreadable] [unreadable] [unreadable]..
  60. Translational Study of Hostility, Atherothrombosis & CAD
    Daichi Shimbo; Fiscal Year: 2007
    ..Data from this translational research program will allow Dr. Shimbo to establish an empirical foundation from which to apply these data to the development of effective preventive interventions for hostile patients. ..
  61. Platelet ADP receptors
    Satya Kunapuli; Fiscal Year: 2008
    ..We further propose to evaluate the relative contribution of the P2Y1 and the P2Y12 receptors to thrombus formation, using P2Y1 or P2Y12 deficient mice and treatment with antagonists and models of in vivo thrombosis and thromboembolism. ..
  62. Heparin like Glycosaminoglycan Oligosaccharides
    Ram Sasisekharan; Fiscal Year: 2004
    ..Similar technologies for DNA and proteins include things like gene chips that have revolutionized the study of these molecules. We hope advancements, like out technology, will do the same thing for HLGAGs. ..