adenine nucleotides

Summary

Top Publications

  1. ncbi 2-5A-dependent RNase molecules dimerize during activation by 2-5A
    B Dong
    Department of Cancer Biology, Cleveland Clinic Foundation, Ohio 44195
    J Biol Chem 270:4133-7. 1995
  2. ncbi Temsirolimus, an mTOR inhibitor, in combination with lower-dose clofarabine as salvage therapy for older patients with acute myeloid leukaemia: results of a phase II GIMEMA study (AML-1107)
    Sergio Amadori
    Department of Haematology, Tor Vergata University Hospital, Roma, Italy Department of Haematology, St George s Hospital, London, UK
    Br J Haematol 156:205-12. 2012
  3. ncbi A membrane network of receptors and enzymes for adenine nucleotides and nucleosides
    Klaus Schicker
    Institute of Pharmacology, Medical University of Vienna, Waehringerstrasse 13a, A 1090 Vienna, Austria
    Biochim Biophys Acta 1793:325-34. 2009
  4. pmc Extracellular 2',3'-cAMP is a source of adenosine
    Edwin K Jackson
    Departments of Pharmacology and Chemical Biology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15219, USA
    J Biol Chem 284:33097-106. 2009
  5. ncbi European development of clofarabine as treatment for older patients with acute myeloid leukemia considered unsuitable for intensive chemotherapy
    Alan K Burnett
    Department of Haematology, Cardiff University School of Medicine, Cardiff CF14 4XN, United Kingdom
    J Clin Oncol 28:2389-95. 2010
  6. pmc Intracellular regulation of human ClC-5 by adenine nucleotides
    Giovanni Zifarelli
    Istituto di Biofisica, Consiglio Nazionale delle Ricerche, Genova, Italy
    EMBO Rep 10:1111-6. 2009
  7. ncbi A convenient and sensitive fluorescence resonance energy transfer assay for RNase L and 2',5' oligoadenylates
    Chandar S Thakur
    Department of Chemistry, Cleveland State University, OH, USA
    Methods Mol Med 116:103-13. 2005
  8. pmc Fluctuations in mitochondrial membrane potential in single isolated brain mitochondria: modulation by adenine nucleotides and Ca2+
    Olga Vergun
    Department of Pharmacology, University of Pittsburgh, Pittsburgh, Pennsylvania 15261, USA
    Biophys J 87:3585-93. 2004
  9. ncbi Soluble guanylate cyclase is allosterically inhibited by direct interaction with 2-substituted adenine nucleotides
    Inez Ruiz-Stewart
    Division of Clinical Pharmacology, Department of Medicine, Thomas Jefferson University, Philadelphia, PA 19107, USA
    Eur J Biochem 269:2186-93. 2002
  10. ncbi Comparative hydrolysis of extracellular adenine nucleotides and adenosine in synaptic membranes from porcine brain cortex, hippocampus, cerebellum and medulla oblongata
    Filip Kukulski
    Department of Biochemistry, Institute of General and Molecular Biology, N Copernicus University of Torun, Torun, Poland
    Brain Res 1030:49-56. 2004

Detail Information

Publications205 found, 100 shown here

  1. ncbi 2-5A-dependent RNase molecules dimerize during activation by 2-5A
    B Dong
    Department of Cancer Biology, Cleveland Clinic Foundation, Ohio 44195
    J Biol Chem 270:4133-7. 1995
    ..These results demonstrate that the catalytically active form of 2-5A-dependent RNase is a homodimer...
  2. ncbi Temsirolimus, an mTOR inhibitor, in combination with lower-dose clofarabine as salvage therapy for older patients with acute myeloid leukaemia: results of a phase II GIMEMA study (AML-1107)
    Sergio Amadori
    Department of Haematology, Tor Vergata University Hospital, Roma, Italy Department of Haematology, St George s Hospital, London, UK
    Br J Haematol 156:205-12. 2012
    ..The acceptable safety profile and the predictive value of target inhibition encourage further investigation of this novel regimen...
  3. ncbi A membrane network of receptors and enzymes for adenine nucleotides and nucleosides
    Klaus Schicker
    Institute of Pharmacology, Medical University of Vienna, Waehringerstrasse 13a, A 1090 Vienna, Austria
    Biochim Biophys Acta 1793:325-34. 2009
    Most cells express more than one receptor plus degrading enzymes for adenine nucleotides or nucleosides, and cellular responses to purines are rarely compatible with the actions of single receptors...
  4. pmc Extracellular 2',3'-cAMP is a source of adenosine
    Edwin K Jackson
    Departments of Pharmacology and Chemical Biology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15219, USA
    J Biol Chem 284:33097-106. 2009
    ....
  5. ncbi European development of clofarabine as treatment for older patients with acute myeloid leukemia considered unsuitable for intensive chemotherapy
    Alan K Burnett
    Department of Haematology, Cardiff University School of Medicine, Cardiff CF14 4XN, United Kingdom
    J Clin Oncol 28:2389-95. 2010
    ..We assessed the second-generation purine nucleoside analog, clofarabine, in two similar phase II studies in this group of patients...
  6. pmc Intracellular regulation of human ClC-5 by adenine nucleotides
    Giovanni Zifarelli
    Istituto di Biofisica, Consiglio Nazionale delle Ricerche, Genova, Italy
    EMBO Rep 10:1111-6. 2009
    ..The residues Tyr 617 and Asp 727, but not Ser 618, are crucial for the potentiation. These results provide a mechanistic and structural framework for the interpretation of nucleotide regulation of a CLC transporter...
  7. ncbi A convenient and sensitive fluorescence resonance energy transfer assay for RNase L and 2',5' oligoadenylates
    Chandar S Thakur
    Department of Chemistry, Cleveland State University, OH, USA
    Methods Mol Med 116:103-13. 2005
    ..The RNA probe used in the FRET assay was designed based on a region of respiratory syncytial genomic RNA. We demonstrate the utility of our FRET assay with several novel biostable analogs of 2-5A...
  8. pmc Fluctuations in mitochondrial membrane potential in single isolated brain mitochondria: modulation by adenine nucleotides and Ca2+
    Olga Vergun
    Department of Pharmacology, University of Pittsburgh, Pittsburgh, Pennsylvania 15261, USA
    Biophys J 87:3585-93. 2004
    ..The effects of adenine nucleotides were abolished by blockade of the adenine nucleotide translocator with carboxyatractyloside, but were ..
  9. ncbi Soluble guanylate cyclase is allosterically inhibited by direct interaction with 2-substituted adenine nucleotides
    Inez Ruiz-Stewart
    Division of Clinical Pharmacology, Department of Medicine, Thomas Jefferson University, Philadelphia, PA 19107, USA
    Eur J Biochem 269:2186-93. 2002
    ..Previous studies demonstrated that 2-substituted adenine nucleotides, including 2-methylthioATP (2MeSATP) and 2-chloroATP (2ClATP), allosterically inhibit guanylate cyclase C, ..
  10. ncbi Comparative hydrolysis of extracellular adenine nucleotides and adenosine in synaptic membranes from porcine brain cortex, hippocampus, cerebellum and medulla oblongata
    Filip Kukulski
    Department of Biochemistry, Institute of General and Molecular Biology, N Copernicus University of Torun, Torun, Poland
    Brain Res 1030:49-56. 2004
    We have investigated the metabolism of extracellular adenine nucleotides and adenosine in porcine brain. The cortex synaptic plasma membranes hydrolyzed ATP to ADP, AMP and adenosine...
  11. ncbi A kinetic study of a ternary cycle between adenine nucleotides
    Edelmira Valero
    Departamento de Quimica Fisica, Escuela Politecnica Superior de Albacete, Universidad de Castilla La Mancha, Albacete, Spain
    FEBS J 273:3598-613. 2006
    ..In the present paper, a kinetic study is made of the behavior of a moiety-conserved ternary cycle between the adenine nucleotides. The system contains the enzymes S-acetyl coenzyme A synthetase, adenylate kinase and pyruvate kinase, and ..
  12. ncbi A new regulatory principle for in vivo biochemistry: pleiotropic low affinity regulation by the adenine nucleotides--illustrated for the glycolytic enzymes of Saccharomyces cerevisiae
    Femke I C Mensonides
    Department of Molecular Cell Physiology, Faculty of Earth and Life Sciences, VU University Amsterdam, De Boelelaan 1085, 1081 HV Amsterdam, The Netherlands
    FEBS Lett 587:2860-7. 2013
    ..We determined systematically the extent to which the enzyme activities depend on the concentrations of the adenine nucleotides. We found that the effects of the adenine nucleotides on enzymes catalysing reactions in which they are not ..
  13. ncbi Phase II study of clofarabine monotherapy in previously untreated older adults with acute myeloid leukemia and unfavorable prognostic factors
    Hagop M Kantarjian
    University of Texas M D Anderson Cancer Center, Houston, TX 77030, USA
    J Clin Oncol 28:549-55. 2010
    ..This phase II study assessed clofarabine monotherapy in older adults (>or= 60 years of age) with untreated acute myeloid leukemia (AML) and at least one unfavorable baseline prognostic factor...
  14. pmc The 2',3'-cAMP-adenosine pathway
    Edwin K Jackson
    Dept of Pharmacology and Chemical Biology, University of Pittsburgh School of Medicine, 100 Technology Drive, Pittsburgh, PA 15219, USA
    Am J Physiol Renal Physiol 301:F1160-7. 2011
    ....
  15. pmc Trinitrophenyl derivatives bind differently from parent adenine nucleotides to Ca2+-ATPase in the absence of Ca2+
    Chikashi Toyoshima
    Institute of Molecular and Cellular Biosciences, University of Tokyo, Tokyo 113 0032, Japan
    Proc Natl Acad Sci U S A 108:1833-8. 2011
    Trinitrophenyl derivatives of adenine nucleotides are widely used for probing ATP-binding sites...
  16. ncbi A comparison of the transportability, and its role in cytotoxicity, of clofarabine, cladribine, and fludarabine by recombinant human nucleoside transporters produced in three model expression systems
    Karen M King
    Department of Oncology, Cross Cancer Institute, 11560 University Avenue, Edmonton, AB, Canada, T6G 1Z2
    Mol Pharmacol 69:346-53. 2006
    ..These results suggest that the nature and activity of the plasma membrane proteins capable of inward transport of nucleosides are important determinants of Cl-F-ara-A activity in human cells...
  17. ncbi A distinct sequence in the adenine nucleotide translocase from Artemia franciscana embryos is associated with insensitivity to bongkrekate and atypical effects of adenine nucleotides on Ca2+ uptake and sequestration
    Csaba Konràd
    Department of Medical Biochemistry, Semmelweis University, Budapest, Hungary
    FEBS J 278:822-36. 2011
    ..Here, we report that adenine nucleotides decreased, whereas carboxyatractyloside increased, Ca(2+) uptake capacity in mitochondria isolated from ..
  18. ncbi Enzymes that hydrolyze adenine nucleotides in diabetes and associated pathologies
    Gilberto Inácio Lunkes
    Departamento de Quimica, Centro de Ciencias Naturais e Exatas, Universidade Federal de Santa Maria, 97105 900, Santa Maria, RS, Brazil
    Thromb Res 109:189-94. 2003
    ..Probably, such alterations are involved in compensatory physiological responses in these diseases and are related to other important mechanisms of thromboregulation...
  19. ncbi Genetic analysis of Japanese patients with persistent hyperinsulinemic hypoglycemia of infancy: nucleotide-binding fold-2 mutation impairs cooperative binding of adenine nucleotides to sulfonylurea receptor 1
    Y Tanizawa
    Third Department of Internal Medicine, Yamaguchi University School of Medicine, Ube, Japan
    Diabetes 49:114-20. 2000
    ..The genetic etiology of PHHI appears to be heterogeneous. SUR1 mutations may account for no more than 20% of PHHI cases in Japanese patients. Mutations of Kir6.2 and glutamate dehydrogenase genes are likely to be even less common...
  20. ncbi Structural characterization of adenine nucleotides bound to Escherichia coli adenylate kinase. 2. 31P and 13C relaxation measurements in the presence of cobalt(II) and manganese(II)
    Y Lin
    Department of Physics, Indiana University Purdue University Indianapolis, Indiana 46202 3273, USA
    Biochemistry 39:3647-55. 2000
    ..These conformations differ significantly from the nucleotide conformations in crystals of AKe. AP(5)A and AKe.AMP.AMPPNP as determined by X-ray crystallography...
  21. ncbi Clofarabine in pediatric acute leukemia: current findings and issues
    Nobuko Hijiya
    Northwestern University Feinberg School of Medicine and Division of Pediatric Hematology Oncology, Children s Memorial Hospital, Chicago, Illinois 60614 3394, USA
    Pediatr Blood Cancer 59:417-22. 2012
    ..In this article, we review the development of clofarabine, rationale and history of combination regimens, and their potential roles and toxicities in the treatment of pediatric ALL that are important to treating clinicians...
  22. ncbi Effect of ribose supplementation on resynthesis of adenine nucleotides after intense intermittent training in humans
    Y Hellsten
    Human Physiology, Copenhagen Muscle Research Centre, Institute for Excersize and Sport Science, DK 2100 Copenhagen, Denmark
    Am J Physiol Regul Integr Comp Physiol 286:R182-8. 2004
    The effect of oral ribose supplementation on the resynthesis of adenine nucleotides and performance after 1 wk of intense intermittent exercise was examined. Eight subjects performed a random double-blind crossover design...
  23. pmc A CBS domain-containing pyrophosphatase of Moorella thermoacetica is regulated by adenine nucleotides
    Joonas Jämsen
    Department of Biochemistry and Food Chemistry, University of Turku, FIN 20014 Turku, Finland
    Biochem J 408:327-33. 2007
    ..Our results suggest that soluble nucleotide-regulated PPases act as amplifiers of metabolism in bacteria by enhancing or suppressing ATP production and biosynthetic reactions at high and low [ATP]/([AMP]+[ADP]) ratios respectively...
  24. ncbi Clofarabine in leukemia
    Hady Ghanem
    Department of Leukemia, The University of Texas, MD Anderson Cancer Center, 1515 Holcombe Blvd, Houston, TX 77030, USA
    Expert Rev Hematol 3:15-22. 2010
    ..This article describes the development, pharmacology and clinical activity of clofarabine, as well as its emerging role in the treatment of acute leukemia, myelodysplastic syndrome and solid tumors...
  25. ncbi Structural characterization of adenine nucleotides bound to Escherichia coli adenylate kinase. 1. Adenosine conformations by proton two-dimensional transferred nuclear Overhauser effect spectroscopy
    Y Lin
    Department of Physics, Indiana University Purdue University Indianapolis IUPUI, Indiana 46202 3273, USA
    Biochemistry 39:3636-46. 2000
    ..The glycosidic torsion angles, chi, deduced for the adenine nucleotides in these complexes are 51 degrees, 37 degrees, 49 degrees, and 47 degrees, respectively, with an ..
  26. ncbi The 100-kDa 2',5'-oligoadenylate synthetase catalyzing preferentially the synthesis of dimeric pppA2'p5'A molecules is composed of three homologous domains
    D Rebouillat
    Unité de Virologie et Imunologie Cellulaire, ERS CNRS 572, Institut Pasteur, 75724 Paris Cedex 15, France
    J Biol Chem 274:1557-65. 1999
    ..These results confirm that the enzymatic activity of p100 is distinct compared with that of p40/p46 and p69/p71...
  27. pmc Receptor-promoted exocytosis of airway epithelial mucin granules containing a spectrum of adenine nucleotides
    Silvia M Kreda
    Cystic Fibrosis Pulmonary Research and Treatment Center, University of North Carolina at Chapel Hill, 4029A Thurston Bowles Building, Chapel Hill, NC 27599 7248, USA
    J Physiol 588:2255-67. 2010
    ....
  28. ncbi Nuclear accumulation of G-actin in isolated rat hepatocytes by adenine nucleotides
    I Meijerman
    Division of Toxicology, Leiden Amsterdam Center for Drug Research, Leiden University, The Netherlands
    Biochem Biophys Res Commun 240:697-700. 1997
    ..These observations suggest that nuclear accumulation of G-actin is mediated by adenosine receptors...
  29. ncbi Effect of adenine nucleotides on cyclooxygenase and lipoxygenase enzyme products of arachidonic acid in human platelets
    Y P Dragan
    Department of Pharmacology and Toxicology, Medical College of Virginia, Richmond 23298
    Biochem Pharmacol 39:27-32. 1990
    ..This suggests that the 12-lipoxygenase enzyme of platelets can be regulated by adenine nucleotides. We next determined the portion of the nucleotide molecule responsible for the enhanced 12-lipoxygenase ..
  30. ncbi Dynamics of energy charge and adenine nucleotides during uncoupling of catabolism and anabolism in Penicillium ochrochloron
    Pamela Vrabl
    University of Innsbruck, Institute of Microbiology, Technikerstrasse 25, 6020 Innsbruck, Austria
    Mycol Res 113:1422-32. 2009
    ..A possible connection between the change in adenine nucleotide concentrations and the purine salvage pathway is discussed...
  31. pmc A randomized study of clofarabine versus clofarabine plus low-dose cytarabine as front-line therapy for patients aged 60 years and older with acute myeloid leukemia and high-risk myelodysplastic syndrome
    Stefan Faderl
    Department of Leukemia, University of Texas M D Anderson Cancer Center, Houston, TX 77030, USA
    Blood 112:1638-45. 2008
    ..4 months vs 5.8 months; P = .1). Clofarabine plus low-dose cytarabine has a higher response rate than clofarabine alone with comparable toxicity. This trial is registered at www.clinicaltrials.gov as no. NCT00088218...
  32. ncbi Adenine nucleotides decrease the apparent Km of endogenous natriuretic peptide receptors for GTP
    Laura K Antos
    Dept of Biochemistry, Molecular Biology and Biophysics, Univ of Minnesota, 6 155 Jackson, 321 Church St SE, Minneapolis, MN 55455, USA
    Am J Physiol Endocrinol Metab 293:E1756-63. 2007
    ..We conclude that ATP is not required for the initial activation of NPRs but does increase activity over time by reducing the apparent K(m) for GTP...
  33. ncbi Results of a phase 1-2 study of clofarabine in combination with cytarabine (ara-C) in relapsed and refractory acute leukemias
    Stefan Faderl
    Department of Leukemia, Box 428, The University of Texas M D Anderson Cancer Center, 1515 Holcombe Blvd, Houston, TX 77030, USA
    Blood 105:940-7. 2005
    ..The combination of clofarabine with ara-C is safe and active. Cellular pharmacology data support the biochemical modulation strategy...
  34. pmc RNase L inhibitor is induced during human immunodeficiency virus type 1 infection and down regulates the 2-5A/RNase L pathway in human T cells
    C Martinand
    Institut de génétique moléculaire de Montpellier UMR 5535, CNRS Université de Montpellier II, 34293 Montpellier Cedex 5, France
    J Virol 73:290-6. 1999
    ..These findings demonstrate that the level of RLI, via its modulation of RNase L activity, can severely impair HIV replication and suggest the involvement of RLI in the inhibition of the 2-5A/RNase L system observed during HIV infection...
  35. ncbi Modulation of murine dendritic cell function by adenine nucleotides and adenosine: involvement of the A(2B) receptor
    Abduelhakem Ben Addi
    Institute of Interdisciplinary Research, IRIBHM, Universite Libre de Bruxelles, Gosselies, Belgium
    Eur J Immunol 38:1610-20. 2008
    ..receptor; since there is no functional P2Y(11) gene in the murine genome, we investigated the action of adenine nucleotides on murine DC...
  36. ncbi Clofarabine, a novel nucleoside analog, is active in pediatric patients with advanced leukemia
    Sima Jeha
    Department of Hematology Oncology, St Jude Children s Research Hospital, 332 N Lauderdale St, Memphis, TN 38105, USA
    Blood 103:784-9. 2004
    ..Clofarabine is well tolerated and shows significant antileukemic activity in heavily pretreated children. Multicenter phase 2 trials in pediatric acute lymphoblastic leukemia (ALL) and acute myeloid leukemia (AML) are ongoing...
  37. ncbi Phase 2 clinical and pharmacologic study of clofarabine in patients with refractory or relapsed acute leukemia
    Hagop Kantarjian
    Department of Leukemia, Anderson Cancer Center, Houston, TX, USA
    Blood 102:2379-86. 2003
    ..03). This increased only in responders (median, 1.8-fold; P =.008) after the second clofarabine infusion. In summary, clofarabine is active in acute leukemias and MDS; cellular pharmacokinetics may have prognostic significance...
  38. ncbi The antiinflammatory mechanism of methotrexate depends on extracellular conversion of adenine nucleotides to adenosine by ecto-5'-nucleotidase: findings in a study of ecto-5'-nucleotidase gene-deficient mice
    M Carmen Montesinos
    University of Valencia, Valencia, Spain
    Arthritis Rheum 56:1440-5. 2007
    ....
  39. ncbi Measurement of intracellular metabolites of primary metabolism and adenine nucleotides in chemostat cultivated Penicillium chrysogenum
    U Nasution
    Department of Biotechnology, Delft University of Technology, Julianalaan 67, 2628 BC Delft, The Netherlands
    Biotechnol Bioeng 94:159-66. 2006
    ..Intracellular metabolites of glycolysis, TCA cycle, and adenine nucleotides were measured with isotope dilution mass spectrometry (IDMS) using a U-(13)C-labeled metabolite mix ..
  40. pmc Southwest Oncology Group Study S0530: a phase 2 trial of clofarabine and cytarabine for relapsed or refractory acute lymphocytic leukaemia
    Anjali S Advani
    Hematologic Oncology and Blood Disorders, Taussig Cancer Center, Cleveland Clinic, Cleveland, OH 44195, USA
    Br J Haematol 151:430-4. 2010
    ..Nucleoside transporter expression did not predict outcome. This regimen lacked sufficient activity to warrant further testing...
  41. pmc Calcium-induced conversion of adenine nucleotides to inosine monophosphate in human red cells
    L Almaraz
    Departamento de Fisiologia y Bioquimica, Facultad de Medicina, Universidad de Valladolid, Spain
    J Physiol 407:557-67. 1988
    ..5. These results suggest that the irreversibility of calcium-induced ATP depletion results from irreversible trapping of the adenine nucleotide as IMP, and help explain the mechanism of E cell formation...
  42. ncbi Discrete but simultaneous release of adenine nucleotides and serotonin from mouse megakaryocytes as detected with patch- and carbon-fiber electrodes
    Kazuyoshi Kawa
    Department of Neurophysiology, Tohoku University School of Medicine, 2 1 Seiryo machi, Aoba ku, Sendai 980 8575, Japan
    Am J Physiol Cell Physiol 286:C119-28. 2004
    Using patch- and carbon-fiber electrodes, we studied release phenomena of adenine nucleotides and serotonin from megakaryocytes isolated from the bone marrow of the mouse...
  43. pmc Effects of analogues of adenine nucleotides on increases in intracellular calcium mediated by P2T-purinoceptors on human blood platelets
    D A Hall
    Receptors and Cellular Regulation Research Group, School of Biological Sciences, University of Surrey, Guildford
    Br J Pharmacol 108:728-33. 1993
    1. By use of a number of analogues of adenine nucleotides, the structure-activity relationships of the human platelet receptor for adenosine 5'-diphosphate (ADP) mediating increases in intracellular calcium were investigated, and ..
  44. ncbi Clofarabine: emerging role in leukemias
    Keeran Sampat
    University of Texas, MD Anderson Cancer Center, Leukemia Department, 1515 Holcombe Boulevard, Box 0428, Houston, TX 77030, USA
    Expert Opin Investig Drugs 18:1559-64. 2009
    ..An oral formulation of clofarabine is also currently under development...
  45. ncbi Platelet receptors for adenine nucleotides and thromboxane A2
    Swaminathan Murugappan
    Department of Physiology, Temple University School of Medicine, Philadelphia, Pennsylvania 19140, USA
    Semin Thromb Hemost 30:411-8. 2004
    ..This review focuses on the contribution of different nucleotide receptors and TP receptors to platelet function and their potential as antithrombotic agents...
  46. ncbi Activities of the enzymes that hydrolyze adenine nucleotides in platelets from multiple sclerosis patients
    Roselia Maria Spanevello
    Department of Biochemistry, Institute of Basic Sciences for Health, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil
    J Neurol 257:24-30. 2010
    ..Our results suggest that the alterations in NTPDase, E-NPP, 5'-nucleotidase and ADA may have contributed to the alterations in platelet function in MS by altering the levels of nucleotides and nucleosides in the circulation...
  47. pmc High-chloride concentrations abolish the binding of adenine nucleotides in the mitochondrial ADP/ATP carrier family
    Eva Maria Krammer
    Equipe de Dynamique des Assemblages Membranaires, UMR No 7565, Centre National de la Recherche Scientifique Université Henri Poincaré, Nancy, France
    Biophys J 97:L25-7. 2009
    ..These results strongly suggest that the chloride concentration acts as an electrostatic lock for the mitochondrial AAC family, thereby preventing adenine nucleotides from reaching their dedicated binding sites.
  48. ncbi The effect of stress upon hydrolysis adenine nucleotides in blood serum of rats
    Ana Elisa Böhmer
    Departamento de Bioquimica, Instituto de Ciencias Basicas da Saude, Universidade Federal do Rio Grando do Sul, Ramiro Barcelos, 2600 Anexo, Lab 22, 90035 003 Porto Alegre, RS, Brazil
    Pharmacol Biochem Behav 75:467-71. 2003
    ..In the present study, we investigated the effect of acute stress on the hydrolysis of adenine nucleotides in rat blood serum...
  49. ncbi Methanocarba modification of uracil and adenine nucleotides: high potency of Northern ring conformation at P2Y1, P2Y2, P2Y4, and P2Y11 but not P2Y6 receptors
    Hak Sung Kim
    Molecular Recognition Section, Laboratory of Bioorganic Chemistry, National Institute of Diabetes, Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892 0810, USA
    J Med Chem 45:208-18. 2002
    ..Our results suggest that new nucleotide agonists may be designed on the basis of the (N) conformation that favors selectivity for P2Y1, P2Y2, P2Y4, and P2Y11 receptors...
  50. ncbi Germline mutations in the ribonuclease L gene in families showing linkage with HPC1
    J Carpten
    Cancer Genetics Branch, National Human Genome Research Institute, NIH, Bethesda, Maryland 20892, USA
    Nat Genet 30:181-4. 2002
    ..Thus, germline mutations in RNASEL may be of diagnostic value, and the 2-5A pathway might provide opportunities for developing therapies for those with prostate cancer...
  51. ncbi Biochemical and functional characterization of recombinant Rhodnius prolixus platelet aggregation inhibitor 1 as a novel lipocalin with high affinity for adenosine diphosphate and other adenine nucleotides
    Ivo M B Francischetti
    Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 4 Center Drive, Room 4 126, MSC 0425, National Institutes of Health, Bethesda, Maryland 20892 0425, USA
    Biochemistry 41:3810-8. 2002
    ..In this report, we determine the binding constants and specificity of RPAI-1 for adenine nucleotides and its functional significance...
  52. ncbi Adenine nucleotides inhibit cytokine generation by human mast cells through a Gs-coupled receptor
    Chunli Feng
    Department of Medicine, Brigham and Women s Hospital, Boston, MA 02115, USA
    J Immunol 173:7539-47. 2004
    ....
  53. pmc Homo- and heteroexchange of adenine nucleotides and nucleosides in rat hippocampal slices by the nucleoside transport system
    Beata Sperlagh
    Department of Pharmacology, Institute of Experimental Medicine, Hungarian Academy of Sciences, H 1450 Budapest POB 67, Hungary
    Br J Pharmacol 139:623-33. 2003
    ..liquid chromatography analysis of the effluent showed that the tritium label represented the whole set of adenine nucleotides and nucleosides, and ATP significantly increased the outflow of [(3)H]ATP...
  54. ncbi Role of adenine nucleotides in insulin secretion from MIN6 pseudoislets
    A C Hauge-Evans
    Endocrinology and Reproduction Research Group, Biomedical Sciences, King s College London, Guy s Campus, London Bridge, London SE1 1UL, UK
    Mol Cell Endocrinol 191:167-76. 2002
    ..aqonists have been implicated in intercellular communication between beta-cells, so we investigated whether adenine nucleotides co-released with insulin are responsible for the enhanced secretory responses of pseudoislets...
  55. ncbi Phase I clinical and pharmacology study of clofarabine in patients with solid and hematologic cancers
    Hagop M Kantarjian
    Departments of Leukemia, Experimental Therapeutics, and Breast and Head and Neck, The University of Texas M D Anderson Cancer Center, Houston, TX 77030, USA
    J Clin Oncol 21:1167-73. 2003
    ..To define the maximum-tolerated doses (MTDs) and dose-limiting toxicities (DLTs) of clofarabine, given as a 1-hour infusion daily for 5 days, in patients with solid tumors and with acute leukemia...
  56. ncbi Extracellular adenine nucleotides metabolism in astrocyte cultures from different brain regions
    Marcia R Wink
    Departamento de Bioquimica, Instituto de Ciencias Basicas da Saude, UFRGS, Rua Ramiro Barcelos, 2600 Anexo, CEP 90035 003, Porto Alegre, RS, Brazil
    Neurochem Int 43:621-8. 2003
    ..A higher expression of ecto-5'-nucleotidase was identified by RT-PCR in cerebellum. The differences observed may indicate functional heterogeneity of nucleotides in the brain...
  57. pmc Molecular basis for Kir6.2 channel inhibition by adenine nucleotides
    Bernard Ribalet
    UCLA Cardiovascular Research Laboratory, Department of Physiology, University of California Los Angeles School of Medicine, 90095, USA
    Biophys J 84:266-76. 2003
    ..x, and the sulfonylurea receptor, SURx. Interaction of adenine nucleotides with Kir6.2 positively charged amino acids such as K185 and R201 on the C-terminus causes channel closure...
  58. ncbi Adenosine and adenine nucleotides as regulators of cerebral blood flow: roles of acidosis, cell swelling, and KATP channels
    John W Phillis
    Department of Physiology, School of Medicine, Wayne State University, 540 E Canfield, Detroit MI 48201, USA
    Crit Rev Neurobiol 16:237-70. 2004
    ..The aim of this review is to describe developments in our understanding of the roles that adenosine and the adenine nucleotides play in cerebral blood flow control, with some comparisons to coronary blood flow...
  59. pmc The effect of systemic hypoxia on interstitial and blood adenosine, AMP, ADP and ATP in dog skeletal muscle
    F M Mo
    Department of Physiology, and Institute of Cardiovascular Science and Medicine, University of Hong Kong, Hong Kong
    J Physiol 536:593-603. 2001
    ..effect of moderate systemic hypoxia on the arterial, venous and interstitial concentration of adenosine and adenine nucleotides in the neurally and vascularly isolated, constant-flow perfused gracilis muscles of anaesthetized dogs. 2...
  60. pmc Plant succinic semialdehyde dehydrogenase. Cloning, purification, localization in mitochondria, and regulation by adenine nucleotides
    K B Busch
    Department of Plant Sciences, The Weizmann Institute of Science, Rehovot 76100, Israel
    Plant Physiol 121:589-97. 1999
    ..Plant SSADH may be responsive to mitochondrial energy charge and reducing potential in controlling metabolism of gamma-aminobutyric acid...
  61. ncbi Phase II study of clofarabine in pediatric patients with refractory or relapsed acute lymphoblastic leukemia
    Sima Jeha
    Department of Hematology Oncology, St Jude Children s Research Hospital, Memphis, TN 38105, USA
    J Clin Oncol 24:1917-23. 2006
    ..To evaluate the efficacy and safety of clofarabine, a novel deoxyadenosine analog, in pediatric patients with refractory or relapsed acute lymphoblastic leukemia (ALL)...
  62. ncbi Adenine nucleotides modulate epithelial wound healing in vitro
    A U Dignass
    Department of Medicine, University of Essen, Germany
    Eur J Clin Invest 28:554-61. 1998
    b>Adenine nucleotides have been demonstrated to enhance structural and functional regeneration in experimental renal injury in rats. The mechanism of adenine nucleotide action have not been elucidated...
  63. ncbi An ion-pair reversed-phase HPLC method for determination of fresh tissue adenine nucleotides avoiding freeze-thaw degradation of ATP
    Stephanie zur Nedden
    Department of Biological Sciences, University of Warwick, Coventry CV47AL, UK
    Anal Biochem 388:108-14. 2009
    ..can be obtained from high-performance liquid chromatography (HPLC) determination of tissue levels of adenine nucleotides (ATP, ADP, and AMP) and their interrelationship in the tissue energy charge (EC)...
  64. pmc Clofarabine combinations as acute myeloid leukemia salvage therapy
    Stefan Faderl
    Department of Leukemia, The University of Texas M D Anderson Cancer Center, Houston, Texas 77030, USA
    Cancer 113:2090-6. 2008
    ..Clofarabine is a nucleoside analog with activity in adult AML. Combinations with cytarabine in AML are feasible and effective. Idarubicin is another active AML drug, which has not yet been tested with clofarabine...
  65. ncbi Measurement of adenine nucleotides in plasma
    Mark W Gorman
    Department of Physiology and Biophysics, University of Washington, Seattle, WA 98195 7290, USA
    Luminescence 18:173-81. 2003
    ..Measurement of ATP and haemoglobin from lysed erythrocytes revealed that haemolysis exerts a strong influence on plasma ATP concentration that must be taken into account...
  66. ncbi 2',5'-oligoadenylate synthetase from a lower invertebrate, the marine sponge Geodia cydonium, does not need dsRNA for its enzymatic activity
    Annika Lopp
    Laboratory of Molecular Genetics, National Institute of Chemical Physics and Biophysics, Akadeemia tee 23, 12618, Tallinn, Estonia
    Biochim Biophys Acta 1590:140-9. 2002
    ..By its product profile and kinetics of formation, the sponge 2-5A synthetase behaves like a specific isoform of enzymes of the 2-5A synthetase family...
  67. ncbi Inhibitory effects of adenine nucleotides and related substances on UDP-glucuronosyltransferase: structure-effect relationships and evidence for an allosteric mechanism
    Yoshio Nishimura
    Graduate School of Pharmaceutical Sciences, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka 812 8582, Japan
    Biochim Biophys Acta 1770:1557-66. 2007
    ..These results suggest that (1) a number of cellular nucleotides present within the endoplasmic reticulum regulate UGT function; and (2) these substances bind to a common allosteric site on UGT to reduce catalytic function...
  68. ncbi Epigenetic regulation of the binding activity of translation inhibitory proteins that bind the 3' untranslated region of beta-F1-ATPase mRNA by adenine nucleotides and the redox state
    José M Izquierdo
    Departamento de Biologia Molecular, Centro de Biologia Molecular Severo Ochoa, C S I C U A M, Universidad Autonoma de Madrid, 28049 Madrid, Spain
    Arch Biochem Biophys 433:481-6. 2005
    ..Interestingly, the availability of adenine nucleotides regulates in a concentration-dependent manner the binding activities of 3'beta FBPs...
  69. ncbi Opposite effects of uracil and adenine nucleotides on the survival of murine cardiomyocytes
    Alessia Mazzola
    Laboratory of Molecular and Cellular Pharmacology of Purinergic Transmission, Department of Pharmacological Sciences, University of Milan, Milan, Italy
    J Cell Mol Med 12:522-36. 2008
    ..Exposure of cardiomyocytes to adenine nucleotides (ATP, ADP or BzATP) induced apoptosis and necrosis, as determined by flow-cytometry...
  70. ncbi Biochemical characterization of the human RAD51 protein. III. Modulation of DNA binding by adenosine nucleotides
    Gregory Tombline
    Genetics and Molecular Biology Program, Department of Microbiology and Immunology, Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
    J Biol Chem 277:14434-42. 2002
    ..These observations suggest that other factors may assist hRAD51 in order to mirror RecA recombinational function...
  71. ncbi Stimulation of mouse cultured sympathetic neurons by uracil but not adenine nucleotides
    W Nörenberg
    Pharmakologisches Institut, Universitat Freiburg, Hermann Herder Strasse 5, D 79104 Freiburg i Br, Germany
    Neuroscience 103:227-36. 2001
    ..The absence of functional P2X receptors makes the mouse neurons suitable for further study of the uracil nucleotide-sensitive P2Y receptors...
  72. pmc Oligomeric structure of Escherichia coli primary replicative helicase DnaB protein
    W Bujalowski
    Department of Human Biological Chemistry and Genetics, University of Texas Medical Branch, Galveston 77555 0653
    J Biol Chem 269:31350-8. 1994
    ....
  73. ncbi HPC1/RNASEL mediates apoptosis of prostate cancer cells treated with 2',5'-oligoadenylates, topoisomerase I inhibitors, and tumor necrosis factor-related apoptosis-inducing ligand
    Krishnamurthy Malathi
    Department of Cancer Biology, Lerner Research Institute, and Experimental Therapeutics Program, Taussig Cancer Center, The Cleveland Clinic Foundation, Cleveland, Ohio, USA
    Cancer Res 64:9144-51. 2004
    ..These findings indicate that RNase L integrates and amplifies apoptotic signals generated during treatment of prostate cancer cells with 2-5A, Topo I inhibitors, and TRAIL...
  74. pmc Interferon action and apoptosis are defective in mice devoid of 2',5'-oligoadenylate-dependent RNase L
    A Zhou
    Department of Cancer Biology, Lerner Research Institute, Cleveland Clinic Foundation, OH 44195, USA
    EMBO J 16:6355-63. 1997
    ..Another implication is that the 2-5A system is likely to contribute to the antiviral activity of interferon by inducing apoptosis of infected cells...
  75. ncbi Chemotaxis of rat mast cells toward adenine nucleotides
    M A McCloskey
    Department of Zoology and Genetics, Iowa State University, Ames 50011, USA
    J Immunol 163:970-7. 1999
    ..These findings suggest that adenine nucleotides liberated from parasite-infested tissue could participate in the recruitment of mast cells by intestinal ..
  76. ncbi Anion permeability and conduction of adenine nucleotides through a chloride channel in cardiac sarcoplasmic reticulum
    S Kawano
    Department of Cardiovascular Diseases, Medical Research Institute, Tokyo Medical and Dental University, 1 5 45, Yushima Bunkyo ku Tokyo, Japan
    J Biol Chem 274:2085-92. 1999
    ..To understand its function further, we examined the permeation of various anions and adenine nucleotides using the planar lipid bilayer-vesicle fusion technique...
  77. pmc Adenine nucleotides inhibit recombinant N-type calcium channels via G protein-coupled mechanisms in HEK 293 cells; involvement of the P2Y13 receptor-type
    Kerstin Wirkner
    Rudolf Boehm Institute of Pharmacology and Toxicology, University of Leipzig, Haertelstrasse 16 18, Leipzig D 04107, Germany
    Br J Pharmacol 141:141-51. 2004
    ..6. Hence, it is tempting to speculate that P2Y(13) receptors may inhibit N-type Ca(2+) channels via the betagamma subunits of the activated G(i) protein...
  78. ncbi Purine salvage to adenine nucleotides in different skeletal muscle fiber types
    J J Brault
    Department of Physiology, College of Medicine, Department of Biomedical Sciences, College of Veterinary Medicine, and Dalton Cardiovascular Research Center, University of Missouri, Columbia, Missouri 65211, USA
    J Appl Physiol 91:231-8. 2001
    ..In the absence of ribose supplementation, purine salvage rates are relatively low, especially compared with the AdN pool size in skeletal muscle...
  79. ncbi AMP does not induce torpor
    Steven J Swoap
    Department of Biology, Williams College, Williamstown, MA 01267, USA
    Am J Physiol Regul Integr Comp Physiol 293:R468-73. 2007
    ..The maximum rate of T(b) fall into hypothermia was significantly faster with injection of adenine nucleotides (AMP: -0.24 +/- 0.03; ADP: -0.24 +/- 0.02; ATP: -0.25 +/- 0...
  80. pmc Adenylate-coupled ion movement. A mechanism for the control of nodule permeability to O2 diffusion
    Hui Wei
    Department of Biology, Queen s University, Kingston, Ontario, K7L 3N6, Canada
    Plant Physiol 141:280-7. 2006
    ....
  81. ncbi Ecto-hydrolysis of adenine nucleotides in rat blood platelets are altered by ovariectomy
    Daniela Pochmann
    Departamento de Bioquimica, Instituto de Ciencias Basicas da Saude, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil
    Platelets 16:334-9. 2005
    ..The process of haemostasis can also be affected by adenine nucleotides and adenosine...
  82. ncbi In vitro adenine nucleotide catabolism in African catfish spermatozoa
    Marek S Zietara
    Gdansk University, Biological Station, 26 Ornitologów Street, PL 80 680 Gdańsk Sobieszewo, Poland
    Comp Biochem Physiol B Biochem Mol Biol 138:385-9. 2004
    ..One of the possible explanations for this phenomenon is that the spermatozoa actively catabolize adenine nucleotides. A relatively high rate of such catabolism could then contribute to the low ATP concentration and low ..
  83. ncbi In vitro effect of homocysteine on nucleotide hydrolysis by blood serum from adult rats
    Ana Elisa Böhmer
    Departamento de Bioquimica, Instituto de Ciencias Basicas da Saude, Universidade Federal do Rio Grande do Sul, Rua Ramiro Barcelos, 2600 Anexo, CEP 90035 003, Porto Alegre, RS, Brazil
    Chem Biol Interact 160:159-64. 2006
    ..to adenosine by soluble nucleotidases constitutes one of the systems for rapid inactivation of circulating adenine nucleotides. Thus, the main objective of this study was to evaluate if Hcy can participate in the modulation of the ..
  84. pmc Glycogen synthase kinase 3 inhibition slows mitochondrial adenine nucleotide transport and regulates voltage-dependent anion channel phosphorylation
    Samarjit Das
    Department of Pathology, Johns Hopkins University, Baltimore, MD 21205, USA
    Circ Res 103:983-91. 2008
    ..Both proteomics and adenine nucleotide transport data suggest that GSK regulates VDAC and that VDAC may be an important regulatory site in ischemia/reperfusion injury...
  85. ncbi The ice worm, Mesenchytraeus solifugus, elevates adenylate levels at low physiological temperature
    Michael J Napolitano
    Biology Department, The State University of New Jersey, Rutgers, Camden, NJ 08102, USA
    Comp Biochem Physiol A Mol Integr Physiol 137:227-35. 2004
    ..These results suggest that ice worms have evolved a compensatory mechanism by which gains in adenylate nucleotides off-set, at least in part, the inherent lethargy and death usually associated with cold temperature...
  86. ncbi Chronic treatment with lithium increases the ecto-nucleotidase activities in rat hippocampal synatosomes
    Leandre Carmem Wilot
    Departamento de Bioquimica, Instituto de Ciencias Basicas da Saude, UFRGS, Rua Ramiro Barcellos 2600 Anexo, 90035 003, Porto Alegre, RS, Brasil
    Neurosci Lett 368:167-70. 2004
    ..In conclusion, the modulation of the ecto-nucleotidase pathway may be a new explanation for the potential neuroprotective lithium action in hippocampal lesions...
  87. ncbi AMP deamination delays muscle acidification during heavy exercise and hypoxia
    Bernard Korzeniewski
    Faculty of Biotechnology, Jagiellonian University, 30 387 Krakow, Poland
    J Biol Chem 281:3057-66. 2006
    ..The resultant reduction of proton production by anaerobic glycolysis enables us to delay the termination of exercise because of fatigue and/or to diminish cell damage...
  88. ncbi The structure of human deoxycytidine kinase in complex with clofarabine reveals key interactions for prodrug activation
    Yan Zhang
    Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853, USA
    Acta Crystallogr D Biol Crystallogr 62:133-9. 2006
    ..The interactions between 2-Cl and its surrounding hydrophobic residues contribute to the high catalytic efficiency of dCK for clofarabine...
  89. ncbi Hyperthyroidism modifies ecto-nucleotidase activities in synaptosomes from hippocampus and cerebral cortex of rats in different phases of development
    Alessandra Nejar Bruno
    Departamento de Bioquimica, Instituto de Ciencias Basicas da Saude, Universidade Federal do Rio Grande do Sul, Avenida Ramiro Barcellos 2600 ANEXO, 90035 003, Porto Alegre, RS, Brazil
    Int J Dev Neurosci 21:401-8. 2003
    ..Thus, hyperthyroidism affects the extracellular nucleotides balance and adenosine production, interfering in neurotransmitter release, development and others physiological processes in different systems...
  90. ncbi Interstitial purine metabolites in hearts with LV remodeling
    Andrey V Gourine
    Research Institute of Cardiology, Minsk, Republic of Belarus
    Am J Physiol Heart Circ Physiol 286:H677-84. 2004
    ..These results suggest that the decreased myocardial ATP level in hearts with post-MI LV remodeling may be caused by the chronic depletion of the total adenine nucleotide pool...
  91. pmc Adenine nucleotide changes in the remnant liver: An early signal for regeneration after partial hepatectomy
    Sara Crumm
    Department of Pathology, Anatomy and Cell Biology, Thomas Jefferson University, Philadelphia, PA, USA
    Hepatology 48:898-908. 2008
    ..in adenosine diphosphate (ADP) and adenosine monophosphate (AMP), resulting in a marked decline in total adenine nucleotides (TAN). We found no evidence of mitochondrial damage or uncoupling of oxidative phosphorylation...
  92. ncbi Adenine nucleotides in snail muscles as one of biomarkers of fluoride toxicity
    Monika Rac
    Department of Biochemistry and Chemistry, Pomeranian Medical University, Szczecin, Poland
    J Environ Monit 7:631-4. 2005
    ..on the energy balance of the snail's foot was investigated based on measurements of concentrations of adenine nucleotides and their metabolism degradation products...
  93. ncbi Ovariectomy and estradiol replacement therapy alters the adenine nucleotide hydrolysis in rat blood serum
    Daniela Pochmann
    Departamento de Bioquimica, Instituto de Ciencias Basicas da Saude, Universidade Federal do Rio Grande do Sul, Avenida Ramiro Barcellos, 2600 Anexo, 90035 003 Porto Alegre, RS, Brazil
    Thromb Res 114:275-81. 2004
    ..The process of haemostasis and thrombus formation can be also affected by adenine nucleotides and adenosine...
  94. ncbi Compartmentation of energy metabolism in atrial myocardium of patients undergoing cardiac surgery
    Evelin Seppet
    Department of Pathophysiology, Human Genetics and Biology and Cardiovascular and Thoracic Surgery, Centre of Molecular and Clinical Medicine, Faculty of Medicine, University of Tartu, Tartu, Estonia
    Mol Cell Biochem 270:49-61. 2005
    ..coupled to oxidative phosphorylation, the MM-CK is coupled to ATPases and there exists a direct transfer of adenine nucleotides between mitochondria and ATPases...
  95. ncbi Proline induces alterations on nucleotide hydrolysis in synaptosomes from cerebral cortex of rats
    Daniela Delwing
    Departamento de Bioquimica, Instituto de Ciencias Basicas da Saude, Universidade Federal do Rio Grande do Sul, Rua Ramiro Barcelos, 2600 Anexo, Porto Alegre, RS, Brazil
    Brain Res 1149:210-5. 2007
    ..according to our results, it seems reasonable to postulate that proline administration alters the hydrolysis of ATP and probably affects the responses mediated by adenine nucleotides in the central nervous system of proline treated rats.
  96. ncbi Purine level regulation during energy depletion associated with graded excitatory stimulation in brain
    P Nickolas Shepel
    Department of Pharmacology and Therapeutics, University of Manitoba Faculty of Medicine, Winnipeg, Manitoba, R3E 0W3, Canada
    Neurol Res 27:139-48. 2005
    ..However, adenosine at high levels may reflect merely the results of obstructed energy metabolism...
  97. ncbi Hypoxic conformance of metabolism in primary rat hepatocytes: a model of hepatic hibernation
    Ram M Subramanian
    Section of Gastroenterology and Hepatology, The University of Chicago, 5841 S Maryland Avenue, Chicago, IL 60637, USA
    Hepatology 45:455-64. 2007
    ..After reoxygenation, cells exposed to prolonged moderate hypoxia demonstrated a more rapid recovery of respiration compared to acute hypoxia/anoxia...
  98. ncbi Oxygen treatment restores energy status following experimental neonatal hypoxia-ischemia
    John W Calvert
    Department of Physiology, Loma Linda University Medical Center, Loma Linda, CA, USA
    Pediatr Crit Care Med 8:165-73. 2007
    ..The purpose of this study was to determine whether oxygen treatment could attenuate the alterations in cerebral energy metabolism found in the brain following hypoxia-ischemia...
  99. ncbi Adenosine uptake-dependent C6 cell growth inhibition
    Satoko Ohkubo
    Division of Pharmacology, National Institute of Health Sciences, 1 18 1 Kamiyoga, Setagaya Ku, Tokyo 158 8501, Japan
    Eur J Pharmacol 577:35-43. 2007
    In C6 glioma cells, adenine nucleotides, especially AMP, and adenosine inhibited cell proliferation in time- and concentration-dependent manners...
  100. ncbi Nucleotide recognition by the cytoplasmic domain of the human chloride transporter ClC-5
    Sebastian Meyer
    Department of Biochemistry, University of Zurich, Winterthurer Strasse 190, CH 8057 Zurich, Switzerland
    Nat Struct Mol Biol 14:60-7. 2007
    ..Our results establish the structural and energetic basis for the interaction of ClC-5 with nucleotides and provide a framework for future investigations...
  101. ncbi Quantitating adenylate nucleotides in diverse organisms
    Michael J Napolitano
    Biology Department, 315 Penn Street, Rutgers, The State University of New Jersey, Camden, NJ 08102, USA
    J Biochem Biophys Methods 63:69-77. 2005
    ..g., alga, bacteria, fungi, plant)...

Research Grants63

  1. PURINERGIC AXIS OF CARDIAC BLOOD VESSELS
    IAIN BUXTON; Fiscal Year: 2001
    The goal of the proposed research is to explain the role of extracellular adenine nucleotides, particularly adenosine 5'- triphosphate (ATP), in normal cardiac endothelial cell (EC) function where release of factors that participate in ..
  2. HYPOXIA AND CONTROL OF FETAL BREATHING MOVEMENTS
    Brian J Koos; Fiscal Year: 2010
    Extracellular adenine nucleotides, central adenosine (ADO) A2A receptors, and the parafascicular nuclear complex (Pf) of the thalamus are crucially involved in hypoxic inhibition of fetal breathing (FB)...
  3. Purinergic Regulation of Bone Metabolism
    Bruce Neil Cronstein; Fiscal Year: 2013
    ..generation during bone remodeling using animals deficient in the enzymes that convert extracellular adenine nucleotides to adenosine (NPP-1, TNAP, CD39 and CD73)...
  4. Regulation of guanylyl cyclase A and B by hormones, ATP and phosphorylation
    Lincoln Ross Potter; Fiscal Year: 2013
    ..b>Adenine nucleotides regulate GC-A and GC-B by binding unidentified intracellular high affinity activation and low affinity ..
  5. Insulin Clearance: Candidate and Positional Genetic Determinants
    MARK GOODARZI; Fiscal Year: 2012
    ..Thus, the hypothesis underlying this proposal is that genes involved in the interconversion of adenine nucleotides (AMP, ADP, ATP) and cellular response to these molecules (AMPK system) are determinants of insulin ..
  6. Molecular and functional properties of the TRPM2 catioin channel
    Reinhold Penner; Fiscal Year: 2010
    ....
  7. MOLECULAR GENETICS OF ISOCITRATE DEHYDROGENASES
    Lee McAlister Henn; Fiscal Year: 2011
    ..isocitrate dehydrogenase (IDH) is sensitive to cellular energy levels due to allosteric regulation by adenine nucleotides and NADH...
  8. Adenine Nucleotide Metabolism and Signaling in Mammalian Sperm
    George L Gerton; Fiscal Year: 2013
    ..sperm motility, key sperm functions such as capacitation and hyperactivated motility rely on the use of adenine nucleotides as energy sources and as signaling molecules...
  9. Extracellular ATP as regulator of hypoxia-induced vasa vasorum neovascularization
    Evgenia V Gerasimovskaya; Fiscal Year: 2012
    ..Extracellular adenine nucleotides are increasingly recognized as important regulators of vascular functions...
  10. MEMBRANE-LIMITED METABOLISM IN MAMMALIAN RED CELLS
    Hyun Kim; Fiscal Year: 1990
    ..However, inosine by itself cannot replenish the purine moiety of adenine nucleotides. To turnover adenine nucleotides the red cells must encounter adenosine or adenine...
  11. PATHOGENESIS OF MINERAL DUST TOXICITY
    Agnes Kane; Fiscal Year: 1991
    ..by hydroxyl radicals or lipid peroxides leading to activation of poly (ADP-ribose) polymerase, depletion of adenine nucleotides, and cell death...
  12. ALLOSTERIC INHIBITION OF GUANYLYL CYCLASE BY NUCLEOTIDES
    Scott Waldman; Fiscal Year: 2000
    ..Indeed, 2-substituted adenine nucleotides prevent the activation of G Cyclase-C by ST in an allosteric and guanine nucleotide-dependent fashion...
  13. BIOLOGY OF ATRIAL NATRIURETIC FACTOR AND ITS RECEPTORS
    Thomas Maack; Fiscal Year: 2000
    ..Isolated membranes from the recombinant cells will be used to assess the effects of addition of adenine nucleotides and amiloride on kinetics of ligand binding and activation of guanylate cyclase...
  14. Mechanisms and implications of intracellular Zn2+ release in acidified neurons
    Lech Kiedrowski; Fiscal Year: 2013
    ..We will determine the role of low affinity Zn2+ ligands, such as adenine nucleotides, in these [Zn2+]i elevations and establish whether neuronal viability can be improved by a specific ..
  15. GENETIC APPROACHES TO MITOCHONDRIAL VDAC FUNCTION
    William Craigen; Fiscal Year: 2001
    ..to the control the phosphocreatine circuit in muscle tissue by regulating the flux of phosphocreatine and adenine nucleotides across the outer mitochondrial membrane...
  16. INCREASED MEMBRANE POTENTIAL IN CARCINOMA MITOCHONDRIA
    JOSEPHINE MODICA NAPOLITANO; Fiscal Year: 1999
    ..Specific Aim #2 is to investigate differences between cell types in the concentration and/or movement of adenine nucleotides by determining both cytosolic and mitochondrial adenine nucleotide content and by measuring the activity of ..
  17. Host Cell Killing by EPEC: Central Role in Pathogenesis
    JOHN CRANE; Fiscal Year: 2005
    ..Once released, ATP is broken down to other adenine nucleotides and adenosine. Adenosine itself acts as a potent secretatagogue, i.e...
  18. ADENYLYL CYCLASE BY ADENOSINE 3'-PHOSPHATES
    Roger Johnson; Fiscal Year: 2003
    ..The proposed approaches build on the applicant's experience with adenylyl cyclases, with its regulation by adenine nucleotides, and with the synthesis of unique, labeled and unlabeled nucleotide derivatives targeted to specific ..
  19. Microglia adenine nucleotides and hypoxia
    JYOTI WATTERS; Fiscal Year: 2009
    ..Extracellular adenine nucleotides are important modulators of microglial cell inflammatory capacity, and they are abundant following brain ..
  20. Pharmacology of Antirheumatic Drugs
    Bruce Cronstein; Fiscal Year: 2007
    ..inhibitors that methotrexate promotes, by a mechanism that is only poorly understood, release of adenine nucleotides which are converted to adenosine extracellularly by the action of ecto-5'nucleotidase...
  21. MYOCARDIAL MEMBRANE PATHOPHYSIOLOGY
    KAREN BURTON; Fiscal Year: 1990
    ..acids; alterations in triacylglycerol; electrolyte alterations; structural integrity; function; levels of adenine nucleotides; and enzyme loss...
  22. METABOLISM OF GUANOSINE IN ISCHEMIC MYOCARDIUM
    TIMOTHY GEISBUHLER; Fiscal Year: 1991
    ..The contribution of the guanine nucleotide pool to both maintenance of adenine nucleotides and heart performance under normal and post-ischemic conditions will be determined...
  23. PERICELLULAR NUCLEOTIDE METABOLISM AND VASCULAR RESPONSE
    LINDA SLAKEY; Fiscal Year: 1992
    ..Local release of adenine nucleotides and adenosine, and metabolism of these highly pharmacoactive compounds by cell surface enzymes are major ..
  24. ISCHEMIA INDUCED ALTERATIONS IN MYOCARDIAL MEMBRANES
    KAREN BURTON; Fiscal Year: 1992
    ..products, phospholipid degradation, free fatty acids, electrolyte alterations, myocardial function, adenine nucleotides, enzyme loss, and structural integrity...
  25. MICROPROBE ANALYSIS/METABOLITE LEVELS OF I. MYOCARDIUM
    WOLFGANG MERGNER; Fiscal Year: 1980
    ..of unfixed ultrathin frozen-dried myocardial sections and measurements of metabolite levels such as adenine nucleotides and lactate...
  26. ROLE OF ATP IN VASCULAR NEUROEFFECTOR PROCESS
    DAVID WESTFALL; Fiscal Year: 2006
    ..provided by the applicant): This application is a request for funds to continue studies on the role of adenine nucleotides and nucleosides in sympathetic neuroeffector process...
  27. HETEROGENEITY CELL CALCIUM DISTRIBUTION RENAL HYPOXIA
    EDORIS LE FURGEY; Fiscal Year: 1990
    ..conditions will also be examined that preserve renal function during anoxia, such as the presence of adenine nucleotides, mannitol, and low pH in the bathing medium...
  28. ETHANOL OXIDATION IN GENETICALLY MODIFIED CELLS
    David Crabb; Fiscal Year: 2002
    ..that permit alterations of the substrate and product concentrations, as well as fatty acids, acetate, and adenine nucleotides. The rates of ethanol oxidation will be fit to steady-state rate equations to determine if the enzyme ..
  29. ISCHEMIC AND REPERFUSION INJURY IN CARDIAC HYPERTROPHY
    Peter Anderson; Fiscal Year: 1993
    ..High energy phosphates and intracellular pH will be continuously monitereed by 31P NMR spectroscopy. Adenine nucleotides and breakdown products will be measured by HPLC, and glycogen, lactate, and LDH will be measured by ..
  30. SR Calcium Release Channel Regulation in Aging Muscle
    EDWARD BALOG; Fiscal Year: 2006
    ..To test this hypothesis, Aim 1 will determine whether aging alters the regulation of RyR1 by adenine nucleotides and effector ions; Aim 2 will determine the impact of aging on the functional interaction of RyR1 with the ..
  31. DONOR LIVER VIABILITY ASSESSMENT BY NMR AND MICROSCOPY
    Melvin Clouse; Fiscal Year: 1993
    ..long-term survivor rats will be compared by in-vivo NMR determination of ATP fluxes and HPLC analysis of adenine nucleotides. These experiments will help delineate optimal preservation conditions and sequential changes occurring ..
  32. METABOLIC BASIS OF FUEL SENSING IN PANCREATIC BETA CELLS
    Maria Erecinska; Fiscal Year: 1992
    ..effector mechanism involved in initiating insulin secretion may respond to localized concentrations of adenine nucleotides. This plan is directed toward a long-term goal of understanding the impairment in beta-cell function ..
  33. Structural Investigations of Guanylyl Cyclase Function
    Patricia Pellicena; Fiscal Year: 2004
    ..In particular, the role of the protein kinase-like domain in modulating cyclase activity in response to adenine nucleotides. Constructs suitable for crystallization will be made and biochemically characterized...
  34. ROLE OF ATP IN BLADDER NEUROEFFECTOR FUNCTION
    DAVID WESTFALL; Fiscal Year: 1993
    ..Once the involvement of adenine nucleotides and nucleosides in normal bladder has been more clearly defined, the actions of these purines in bladders ..
  35. MYOCARDIAL ISCHEMIA AND REPERFUSION
    KEITH REIMER; Fiscal Year: 2000
    ..Cardioprotection will be assessed by measuring myocardial infarct size and the rate of ischemic metabolism (rate of change of adenine nucleotides and intermediates of anaerobic glycolysis during ischemia).
  36. Clofarabine: Cytarabine Activation for Leukemia Response
    Stefan Faderl; Fiscal Year: 2004
    ..abstract_text> ..
  37. GRP78/BIP AND ASSISTED PROTEIN FOLDING
    Sylvie Blond; Fiscal Year: 2002
    ..This investigation will characterize the role of specificity for unfolded peptidic substrates, affinity for adenine nucleotides and for accessory proteins in the functions of GRP78/BiP in assisted protein folding.
  38. CYCLIC AMP/ADENOSINE PATHWAY
    Edwin Jackson; Fiscal Year: 2002
    ..abstract_text> ..
  39. Phase I study of 8-Cl-adenosine in CLL (IND 68,229)
    Varsha Gandhi; Fiscal Year: 2010
    ..The investigators state that these Aims will establish utility of 8-Cl-Ado in CLL, provide a dose for Phase 2 study, and knowledge for optimal use of this agent alone and in combination. ..
  40. CHILDRENS CANCER GROUP
    Peter Steinherz; Fiscal Year: 2002
    ..Members of the affiliate hospitals have been participating in CCG studies and have proven their ability to conduct the clinical studies and provide material for laboratory evaluation. ..
  41. BIOCHEMICAL STRATEGIES TO INCREASE LEUKEMIA RESPONSE
    Varsha Gandhi; Fiscal Year: 2005
    ..We feel that knowledge gained through these aims will assist us in designing optimal therapy of leukemia with nucleoside analogs. ..
  42. Mechanisms of Nucleotide Release by Airway Epithelia
    Eduardo Lazarowski; Fiscal Year: 2006
    ..Delineation of nucleotide-releasing mechanisms will provide new possibilities to improve MCC function in chronic lung diseases characterized by mucin-obstructed and inflamed airways. [unreadable] [unreadable]..
  43. UNIQUE AMINO DOMAINS AND AMP DEAMINASE ISOFORM DIVERSITY
    RICHARD SABINA; Fiscal Year: 2002
    ..These combined efforts should provide critical information that may help explain clinical outcomes associated with a prevalent AMPD1 mutant allele. ..
  44. 2-5A Antisense Targeting of Telomerase RNA
    Douglas Leaman; Fiscal Year: 2003
    ..These studies will also impact other antisense products under development by improving the effectiveness of the 2-A antisense technology. ..
  45. Molecular Mediators of Interferon induced Apoptosis
    Douglas Leaman; Fiscal Year: 2005
    ..The results of these studies should provide important information on the molecular details of antitumor activity of IFNs that can be used to identify or develop targeted therapeutics that augment IFN effects. ..
  46. Impact of the interferon regulated proteins XAF1 and ZNF313 on innate immunity
    Douglas W Leaman; Fiscal Year: 2010
    ....
  47. MECHANISM OF ENZYME MEDIATED ACTIVATION OF COENZYME B12
    Kenneth Brown; Fiscal Year: 2005
    ..In addition, the possibility that the active species of RTPR is actually a dimer will be investigated by determining the effect of protein concentration on the observed binding constants for AdoCbl and the allosteric effector dGTP. ..
  48. PATHOPHYSIOLOGY OF NICOTINE IN CEREBRAL BLOOD VESSELS
    J Simard; Fiscal Year: 2004
    ....
  49. High-Accuracy Models of Proteins from Remote Homology
    Jie Liang; Fiscal Year: 2009
    ..These computational capabilities could have profound impacts to drug design and disease studies. ..
  50. FUNCTION ANALYSIS OF THE DSRNA REGULATED NUCLEAR FACTORS
    Glen Barber; Fiscal Year: 2004
    ..abstract_text> ..
  51. DNA BENDING AND CONFORMATIONAL SWITCHING
    J Schurr; Fiscal Year: 2003
    ..In addition, the effects of binding IHF to the ends of a 277 bp DNA will be studied to determine any effect of the large induced bends on the intervening DNA. ..
  52. Hair-Bundle Lipids
    Peter Gillespie; Fiscal Year: 2009
    ..Together, the experiments in this proposal will allow us to determine how lipids regulate transduction and adaptation. ..
  53. P2T PURINERGIC RECEPTORS
    JOSE BOYER; Fiscal Year: 2004
    ..The affects of adenine nucleotides on platelet receptors are strongly influenced by the complex metabolism of extracellular nucleotides by ..
  54. VACCINIA VIRUS DNA TOPOISOMERASE
    Stewart Shuman; Fiscal Year: 2006
    ..5) Biochemical and genetic characterization of the TopIB proteins from Mycobacterium avium, Mycobacterium smegmatis, and Pseudomonas aeurginosa. ..
  55. Effect of Zinc in Enteropathogenic E. coli Infection
    JOHN CRANE; Fiscal Year: 2006
    ..abstract_text> ..
  56. Immunogenicity of Hepatitis C Virus (HCV) like particles
    Glen Barber; Fiscal Year: 2007
    ..abstract_text> ..
  57. Role of PKR in Growth Regulation
    Glen Barber; Fiscal Year: 2008
    ..We propose that these aims will lend insight into mechanisms of cell signaling, tumorigenesis and interferon-mediated actions in the cell. ..
  58. Iron Therapy in Renal Disease: Potential Toxicities
    RICHARD ZAGER; Fiscal Year: 2008
    ..Potential reasons for these differences will be assessed, with the ultimate goal of defining the safest way of administering parenteral irons to renal disease patients. ..
  59. InteractiveSlides - Microarray data visualization tool
    Jozef Spychala; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable] [unreadable]..
  60. THERMODYNAMICS OF BIOCHEMICAL SYSTEMS
    ROBERT ALBERTY; Fiscal Year: 2005
    ..This quantitative treatment of the literature data on biochemical reactions will uncover errors because deltafG'0 values can be determined by use of two or more biochemical reactions, which should give the same answer. ..
  61. Enteropathogenic and Toxigenic E.coli Interactions
    JOHN CRANE; Fiscal Year: 2004
    ..abstract_text> ..
  62. ROLE OF CALCIUM PUMPING IN HAIR-BUNDLE FUNCTION
    Peter Gillespie; Fiscal Year: 2004
    ..If re-expression of PMCA2 in hair cells rescues auditory function in these otherwise PMCA2-deficient mice, the key location of PMCA2 in the ear must be the hair cell. ..
  63. A FADD-Associated Helicase Essential for Host Defense
    Glen Barber; Fiscal Year: 2008
    ..We anticipate that these studies will improve our knowledge of pathogenesis and provide new concepts for the rational design of novel therapeutic and vaccine strategies. [unreadable] [unreadable] [unreadable]..