cyclic gmp

Summary

Summary: Guanosine cyclic 3',5'-(hydrogen phosphate). A guanine nucleotide containing one phosphate group which is esterified to the sugar moiety in both the 3'- and 5'-positions. It is a cellular regulatory agent and has been described as a second messenger. Its levels increase in response to a variety of hormones, including acetylcholine, insulin, and oxytocin and it has been found to activate specific protein kinases. (From Merck Index, 11th ed)

Top Publications

  1. pmc Cyclic GMP-AMP synthase is a cytosolic DNA sensor that activates the type I interferon pathway
    Lijun Sun
    Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
    Science 339:786-91. 2013
  2. pmc Cyclic GMP-AMP is an endogenous second messenger in innate immune signaling by cytosolic DNA
    Jiaxi Wu
    Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
    Science 339:826-30. 2013
  3. ncbi Principles of c-di-GMP signalling in bacteria
    Regine Hengge
    Institut für Biologie Mikrobiologie, Freie Universitat Berlin, 14195 Berlin, Germany
    Nat Rev Microbiol 7:263-73. 2009
  4. ncbi Mechanisms of cyclic-di-GMP signaling in bacteria
    Urs Jenal
    Biozentrum of the University of Basel, CH 4056 Basel, Switzerland
    Annu Rev Genet 40:385-407. 2006
  5. pmc Cell-cell signaling in Xanthomonas campestris involves an HD-GYP domain protein that functions in cyclic di-GMP turnover
    Robert P Ryan
    BIOMERIT Research Centre, Department of Microbiology, Biosciences Institute, National University of Ireland, Cork, Ireland
    Proc Natl Acad Sci U S A 103:6712-7. 2006
  6. pmc Analysis of Pseudomonas aeruginosa diguanylate cyclases and phosphodiesterases reveals a role for bis-(3'-5')-cyclic-GMP in virulence
    Hemantha Kulasakara
    Department of Microbiology and Molecular Genetics, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 103:2839-44. 2006
  7. pmc cGMP-dependent protein kinases and cGMP phosphodiesterases in nitric oxide and cGMP action
    Sharron H Francis
    Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, 2215 Garland Avenue, Nashville, TN 37232 0615, USA
    Pharmacol Rev 62:525-63. 2010
  8. ncbi Structural and mechanistic determinants of c-di-GMP signalling
    Tilman Schirmer
    Biozentrum, University of Basel, Klingelbergstrasse 50, CH 4056 Basel, Switzerland
    Nat Rev Microbiol 7:724-35. 2009
  9. pmc MrkH, a novel c-di-GMP-dependent transcriptional activator, controls Klebsiella pneumoniae biofilm formation by regulating type 3 fimbriae expression
    Jonathan J Wilksch
    Department of Microbiology and Immunology, The University of Melbourne, Parkville, Victoria, Australia
    PLoS Pathog 7:e1002204. 2011
  10. pmc Inverse regulatory coordination of motility and curli-mediated adhesion in Escherichia coli
    Christina Pesavento
    Institut für Biologie Mikrobiologie, Freie Universitat Berlin, 14195 Berlin, Germany
    Genes Dev 22:2434-46. 2008

Detail Information

Publications265 found, 100 shown here

  1. pmc Cyclic GMP-AMP synthase is a cytosolic DNA sensor that activates the type I interferon pathway
    Lijun Sun
    Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
    Science 339:786-91. 2013
    ..DNA induces interferons through the production of cyclic guanosine monophosphate-adenosine monophosphate (cyclic GMP-AMP, or cGAMP), which binds to and activates the adaptor protein STING...
  2. pmc Cyclic GMP-AMP is an endogenous second messenger in innate immune signaling by cytosolic DNA
    Jiaxi Wu
    Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
    Science 339:826-30. 2013
    ..We found that mammalian cytosolic extracts synthesized cyclic guanosine monophosphate-adenosine monophosphate (cyclic GMP-AMP, or cGAMP) in vitro from adenosine triphosphate and guanosine triphosphate in the presence of DNA but not ..
  3. ncbi Principles of c-di-GMP signalling in bacteria
    Regine Hengge
    Institut für Biologie Mikrobiologie, Freie Universitat Berlin, 14195 Berlin, Germany
    Nat Rev Microbiol 7:263-73. 2009
    ..This Review focuses on emerging principles of c-di-GMP signalling using selected systems in different bacteria as examples...
  4. ncbi Mechanisms of cyclic-di-GMP signaling in bacteria
    Urs Jenal
    Biozentrum of the University of Basel, CH 4056 Basel, Switzerland
    Annu Rev Genet 40:385-407. 2006
    ..In addition, we examine several well-established model systems for c-di-GMP signaling, including Pseudomonas, Vibrio, Caulobacter, and Salmonella...
  5. pmc Cell-cell signaling in Xanthomonas campestris involves an HD-GYP domain protein that functions in cyclic di-GMP turnover
    Robert P Ryan
    BIOMERIT Research Centre, Department of Microbiology, Biosciences Institute, National University of Ireland, Cork, Ireland
    Proc Natl Acad Sci U S A 103:6712-7. 2006
    ..Identification of the role of the HD-GYP domain thus increases our understanding of a signaling network whose importance to the lifestyle of diverse bacteria is now emerging...
  6. pmc Analysis of Pseudomonas aeruginosa diguanylate cyclases and phosphodiesterases reveals a role for bis-(3'-5')-cyclic-GMP in virulence
    Hemantha Kulasakara
    Department of Microbiology and Molecular Genetics, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 103:2839-44. 2006
    ....
  7. pmc cGMP-dependent protein kinases and cGMP phosphodiesterases in nitric oxide and cGMP action
    Sharron H Francis
    Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, 2215 Garland Avenue, Nashville, TN 37232 0615, USA
    Pharmacol Rev 62:525-63. 2010
    ..Potential for use of these medications in the treatment of other maladies continues to emerge...
  8. ncbi Structural and mechanistic determinants of c-di-GMP signalling
    Tilman Schirmer
    Biozentrum, University of Basel, Klingelbergstrasse 50, CH 4056 Basel, Switzerland
    Nat Rev Microbiol 7:724-35. 2009
    ..Here, we review recent data on the structure and functional properties of the protein families that are involved in c-di-GMP signalling and discuss the mechanistic implications...
  9. pmc MrkH, a novel c-di-GMP-dependent transcriptional activator, controls Klebsiella pneumoniae biofilm formation by regulating type 3 fimbriae expression
    Jonathan J Wilksch
    Department of Microbiology and Immunology, The University of Melbourne, Parkville, Victoria, Australia
    PLoS Pathog 7:e1002204. 2011
    ..pneumoniae to promote biofilm formation to implanted medical devices...
  10. pmc Inverse regulatory coordination of motility and curli-mediated adhesion in Escherichia coli
    Christina Pesavento
    Institut für Biologie Mikrobiologie, Freie Universitat Berlin, 14195 Berlin, Germany
    Genes Dev 22:2434-46. 2008
    ..In summary, these data reveal the logic and sequence of molecular events underlying the motile-to-adhesive "lifestyle" switch in E. coli...
  11. pmc You've come a long way: c-di-GMP signaling
    Holger Sondermann
    Department of Molecular Medicine, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA
    Curr Opin Microbiol 15:140-6. 2012
    ..Recent studies revealing the molecular basis of c-di-GMP signaling mechanisms enhanced our understanding of how this molecule controls downstream biological processes and how c-di-GMP signaling specificity is achieved...
  12. pmc Cyclic GMP-AMP containing mixed phosphodiester linkages is an endogenous high-affinity ligand for STING
    Xu Zhang
    Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390 9148, USA
    Mol Cell 51:226-35. 2013
    ..The crystal structure of STING bound to 2'3'-cGAMP revealed the structural basis of this high-affinity binding and a ligand-induced conformational change in STING that may underlie its activation. ..
  13. pmc Cell cycle-dependent dynamic localization of a bacterial response regulator with a novel di-guanylate cyclase output domain
    Ralf Paul
    Division of Molecular Microbiology, Biozentrum, University of Basel, 4056 Basel, Switzerland
    Genes Dev 18:715-27. 2004
    ..We propose that the local action of this novel-type guanylate cyclase might constitute a general regulatory principle in bacterial growth and development...
  14. pmc Vibrio cholerae VpsT regulates matrix production and motility by directly sensing cyclic di-GMP
    Petya V Krasteva
    Department of Molecular Medicine, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA
    Science 327:866-8. 2010
    ..Rather than being regulated by phosphorylation, VpsT undergoes a change in oligomerization on c-di-GMP binding...
  15. pmc The c-di-GMP binding protein YcgR controls flagellar motor direction and speed to affect chemotaxis by a "backstop brake" mechanism
    Koushik Paul
    Department of Biology, University of Utah, Salt Lake City, UT 84112, USA
    Mol Cell 38:128-39. 2010
    ..Inhibition of motility and chemotaxis may represent a strategy to prepare for sedentary existence by disfavoring migration away from a substrate on which a biofilm is to be formed...
  16. pmc Analysis of the HD-GYP domain cyclic dimeric GMP phosphodiesterase reveals a role in motility and the enzootic life cycle of Borrelia burgdorferi
    Syed Z Sultan
    Department of Microbiology and Immunology, Brody School of Medicine, East Carolina University, Greenville, NC 27834, USA
    Infect Immun 79:3273-83. 2011
    ..All of these phenotypes were restored when the mutant was complemented. Identification of this role of pdeB increases our understanding of the c-di-GMP signaling network in motility regulation and the life cycle of B. burgdorferi...
  17. ncbi Structural basis for modulation and agonist specificity of HCN pacemaker channels
    William N Zagotta
    Department of Physiology and Biophysics, Howard Hughes Medical Institute, Box 357290, University of Washington School of Medicine, Seattle, Washington 98195 7290, USA
    Nature 425:200-5. 2003
    ..On the basis of amino acid sequence similarity to HCN channels, the cyclic nucleotide-gated, and eag- and KAT1-related families of channels are probably related to HCN channels in structure and mechanism...
  18. ncbi When the PilZ don't work: effectors for cyclic di-GMP action in bacteria
    Robert P Ryan
    BIOMERIT Research Centre, Department of Microbiology, Biosciences Institute, University College Cork, Cork, Ireland
    Trends Microbiol 20:235-42. 2012
    ..Here, we review this range of cyclic di-GMP effectors and the biological processes that they govern using examples from several different bacteria...
  19. pmc A chemosensory system that regulates biofilm formation through modulation of cyclic diguanylate levels
    Jason W Hickman
    Department of Microbiology, Box 357242, University of Washington, 1959 Northeast Pacific Street, Seattle, WA 98195, USA
    Proc Natl Acad Sci U S A 102:14422-7. 2005
    ..Together, the data suggest that the wsp signal transduction pathway regulates biofilm formation through modulation of cyclic diguanylate levels...
  20. ncbi GGDEF and EAL domains inversely regulate cyclic di-GMP levels and transition from sessility to motility
    Roger Simm
    Microbiology and Tumorbiology Center MTC, Karolinska Institutet, Box 280, SE 171 77 Stockholm, Sweden
    Mol Microbiol 53:1123-34. 2004
    ..Thus, the data suggest that c-di-GMP is a novel global second messenger in bacteria the metabolism of which is controlled by GGDEF and EAL domain proteins...
  21. ncbi Second messenger-mediated adjustment of bacterial swimming velocity
    Alex Boehm
    Biozentrum, University of Basel, CH 4056 Basel, Switzerland
    Cell 141:107-16. 2010
    ..These experiments demonstrate that bacteria can modulate flagellar motor output and thus swimming velocity in response to environmental cues...
  22. ncbi Identification and characterization of a cyclic di-GMP-specific phosphodiesterase and its allosteric control by GTP
    Matthias Christen
    Division of Molecular Microbiology, Biozentrum, University of Basel, Klingelbergstrasse 70, 4056 Basel, Switzerland
    J Biol Chem 280:30829-37. 2005
    ....
  23. ncbi PilZ domain is part of the bacterial c-di-GMP binding protein
    Dorit Amikam
    Department of Biotechnology and Environmental Sciences, Tel Hai Academic College Tel Hai, Israel
    Bioinformatics 22:3-6. 2006
    ....
  24. ncbi Riboswitches in eubacteria sense the second messenger cyclic di-GMP
    N Sudarsan
    Howard Hughes Medical Institute, Yale University, New Haven, CT 06520, USA
    Science 321:411-3. 2008
    ..In addition, sequences matching the consensus for cyclic di-GMP riboswitches are present in the genome of a bacteriophage...
  25. pmc Pseudomonas aeruginosa rugose small-colony variants have adaptations that likely promote persistence in the cystic fibrosis lung
    Melissa Starkey
    Department of Microbiology, University of Washington, Seattle, WA 98195 7242, USA
    J Bacteriol 191:3492-503. 2009
    ..RSCV traits such as increased exopolysaccharide production leading to antibiotic tolerance, altered metabolism, and reduced immunogenicity may contribute to increased persistence in biofilms and in the airways of CF lungs...
  26. pmc Asymmetrical distribution of the second messenger c-di-GMP upon bacterial cell division
    Matthias Christen
    Department of Immunology, University of Washington, Seattle, WA 98195, USA
    Science 328:1295-7. 2010
    ..Thus, asymmetrical distribution of c-di-GMP was observed as part of cell division, which may indicate an important regulatory step in extracellular organelle biosynthesis or function...
  27. pmc Cis-2-dodecenoic acid receptor RpfR links quorum-sensing signal perception with regulation of virulence through cyclic dimeric guanosine monophosphate turnover
    Yinyue Deng
    Institute of Molecular and Cell Biology, Singapore
    Proc Natl Acad Sci U S A 109:15479-84. 2012
    ..Our work presents a unique and widely conserved DSF-family signal receptor that directly links the signal perception to c-di-GMP turnover in regulation of bacterial physiology...
  28. ncbi The PilZ domain is a receptor for the second messenger c-di-GMP: the PilZ domain protein YcgR controls motility in enterobacteria
    Dmitri A Ryjenkov
    Department of Molecular Biology, University of Wyoming, Laramie, Wyoming 82071, USA
    J Biol Chem 281:30310-4. 2006
    ..Therefore, biochemical and genetic evidence presented here establishes PilZ as a long sought after c-di-GMP-binding domain and YcgR as a c-di-GMP receptor affecting motility in enterobacteria...
  29. pmc Quantification of high-specificity cyclic diguanylate signaling
    Jonathan P Massie
    Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, MI 48824, USA
    Proc Natl Acad Sci U S A 109:12746-51. 2012
    ..Our results conclusively demonstrate that c-di-GMP does not function via a simple, low-specificity signaling pathway in V. cholerae...
  30. pmc Allosteric activation of exopolysaccharide synthesis through cyclic di-GMP-stimulated protein-protein interaction
    Samuel Steiner
    Biozentrum, University of Basel, 4056 Basel, Switzerland
    EMBO J 32:354-68. 2013
    ..These data uncover a mechanism of c-di-GMP-mediated EPS control and provide a frame for c-di-GMP signalling specificity in pathogenic bacteria...
  31. pmc The ubiquitous protein domain EAL is a cyclic diguanylate-specific phosphodiesterase: enzymatically active and inactive EAL domains
    Andrew J Schmidt
    Department of Molecular Biology, University of Wyoming, Laramie, 82071, USA
    J Bacteriol 187:4774-81. 2005
    ..The enzymatic inactivity of EAL domains may explain their coexistence with GGDEF domains in proteins possessing c-di-GMP synthase (diguanulate cyclase) activity...
  32. pmc LapD is a bis-(3',5')-cyclic dimeric GMP-binding protein that regulates surface attachment by Pseudomonas fluorescens Pf0-1
    Peter D Newell
    Department of Microbiology and Immunology, Dartmouth Medical School, Hanover, NH 03755, USA
    Proc Natl Acad Sci U S A 106:3461-6. 2009
    ..Furthermore, we show that LapD serves as the c-di-GMP receptor connecting environmental modulation of intracellular c-di-GMP levels by inorganic phosphate to regulation of LapA localization and thus surface commitment by P. fluorescens...
  33. ncbi Cyclic-diGMP signal transduction systems in Vibrio cholerae: modulation of rugosity and biofilm formation
    Bentley Lim
    Department of Environmental Toxicology, University of California Santa Cruz, Santa Cruz, CA 95064, USA
    Mol Microbiol 60:331-48. 2006
    ..Furthermore, epistasis analysis suggested that cdgC, rocS and mbaA act in convergent pathways to regulate the phenotypic properties of the rugose and smooth variants, and are part of the VpsR, VpsT and HapR signal transduction pathway...
  34. pmc Cyclic di-GMP allosterically inhibits the CRP-like protein (Clp) of Xanthomonas axonopodis pv. citri
    Jason L Leduc
    Department of Bacteriology, 1550 Linden Drive, University of Wisconsin Madison, Madison, WI 53706, USA
    J Bacteriol 191:7121-2. 2009
    ..We show that unlike the DNA-binding activity of other members of this family, the DNA-binding activity of Clp is allosterically inhibited by its effector and that cyclic di-GMP serves as that effector at physiological concentrations...
  35. ncbi The second messenger bis-(3'-5')-cyclic-GMP and its PilZ domain-containing receptor Alg44 are required for alginate biosynthesis in Pseudomonas aeruginosa
    Massimo Merighi
    Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA 02115, USA
    Mol Microbiol 65:876-95. 2007
    ..Alg44 appears to be an essential component of the alginate biosynthetic apparatus, where, following binding of c-di-GMP, it controls polymerization or transport of the polysaccharide...
  36. pmc Roles of cyclic diguanylate in the regulation of bacterial pathogenesis
    Rita Tamayo
    Howard Hughes Medical Institute, Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, Massachusetts 02111, USA
    Annu Rev Microbiol 61:131-48. 2007
    ..Finally, ways in which c-di-GMP may mediate these regulatory effects are proposed...
  37. pmc Identification of FleQ from Pseudomonas aeruginosa as a c-di-GMP-responsive transcription factor
    Jason W Hickman
    Department of Microbiology, University of Washington, Seattle, WA 98195, USA
    Mol Microbiol 69:376-89. 2008
    ..Our results show that FleQ is a new type of c-di-GMP binding protein that controls the transcriptional regulation of EPS biosynthesis genes in P. aeruginosa...
  38. ncbi Structure and mechanism of a bacterial light-regulated cyclic nucleotide phosphodiesterase
    Thomas R M Barends
    Max Planck Institute for Medical Research, Department of Biomolecular Mechanisms, Jahnstrasse 29, 69120 Heidelberg, Germany
    Nature 459:1015-8. 2009
    ..Photon absorption by the BLUF domain of one subunit of the antiparallel BlrP1 homodimer activates the EAL domain of the second subunit through allosteric communication transmitted through conserved domain-domain interfaces...
  39. ncbi A post-translational, c-di-GMP-dependent mechanism regulating flagellar motility
    Xin Fang
    Department of Molecular Biology, University of Wyoming, Laramie, WY 82071, USA
    Mol Microbiol 76:1295-305. 2010
    ....
  40. ncbi Natriuretic peptides, their receptors, and cyclic guanosine monophosphate-dependent signaling functions
    Lincoln R Potter
    Department of Biochemistry, Molecular Biology, and Biophysics, 6 155 Jackson Hall, 321 Church Street SE, University of Minnesota, Minneapolis, Minnesota 55455, USA
    Endocr Rev 27:47-72. 2006
    ..In this comprehensive review, the structure, function, regulation, and biological consequences of natriuretic peptides and their associated signaling proteins are described...
  41. ncbi Cyclic GMP-gated CNG channels function in Sema3A-induced growth cone repulsion
    Kazunobu Togashi
    Department of Biochemistry, New York University School of Medicine, New York, NY 10016 6402, USA
    Neuron 58:694-707. 2008
    ..These studies extend our knowledge of the function of CNGCs by demonstrating their requirement for signal transduction in growth cone guidance...
  42. pmc The structural basis of cyclic diguanylate signal transduction by PilZ domains
    Jordi Benach
    Department of Biological Sciences, Northeast Structural Genomics Consortium, Columbia University, New York, NY 10027, USA
    EMBO J 26:5153-66. 2007
    ..The very small size of the N-terminal conformational switch is likely to explain the facile evolutionary diversification of the PilZ domain...
  43. pmc N-3-oxo-decanoyl-L-homoserine-lactone activates auxin-induced adventitious root formation via hydrogen peroxide- and nitric oxide-dependent cyclic GMP signaling in mung bean
    Xuegui Bai
    Key Laboratory of Biodiversity and Biogeography, Kunming Institute of Botany, Institute of Tibet Plateau Research at Kunming, Chinese Academy of Sciences, Kunming 650204, China
    Plant Physiol 158:725-36. 2012
    ..Hydrogen peroxide (H(2)O(2)), nitric oxide (NO), and cyclic GMP (cGMP) are essential messengers that participate in various plant physiological processes, but how these ..
  44. pmc The cyclic nucleotide cGMP is involved in plant hormone signalling and alters phosphorylation of Arabidopsis thaliana root proteins
    Jean Charles Isner
    Department of Biology Area 9, University of York, York, YO10 5DD, UK
    J Exp Bot 63:3199-205. 2012
    ..Out of these, nine were previously shown to also alter phosphorylation status in response to plant hormones, pointing to protein phosphorylation as a target for hormone-induced cGMP signalling...
  45. ncbi Molecular identification of a hyperpolarization-activated channel in sea urchin sperm
    R Gauss
    Institut für Biologische Informationsverarbeitung, Forschungszentrum Julich, Germany
    Nature 393:583-7. 1998
    ..chemotactic peptides, which evoke complex changes in membrane voltage and in the concentrations of cyclic AMP, cyclic GMP and Ca2+ ions The intracellular signalling pathways and their cellular targets are largely unknown...
  46. ncbi Chronic inhibition of cyclic GMP phosphodiesterase 5A prevents and reverses cardiac hypertrophy
    Eiki Takimoto
    Division of Cardiology, Department of Medicine, Johns Hopkins Medical Institutions, Ross 835, 720 Rutland Avenue, Baltimore, Maryland 21205, USA
    Nat Med 11:214-22. 2005
    ..PDE5A inhibition may provide a new treatment strategy for cardiac hypertrophy and remodeling...
  47. ncbi c-di-GMP-mediated regulation of virulence and biofilm formation
    Peggy A Cotter
    Department of Molecular, Cellular, and Developmental Biology, University of California, Santa Barbara, Santa Barbara, CA 93106 9610, USA
    Curr Opin Microbiol 10:17-23. 2007
    ....
  48. ncbi An allosteric self-splicing ribozyme triggered by a bacterial second messenger
    Elaine R Lee
    Department of Molecular, Cellular, and Developmental Biology, Yale University, Box 208103, New Haven, CT 06520 8103, USA
    Science 329:845-8. 2010
    ..Our findings indicate that some self-splicing ribozymes are not selfish elements but are harnessed by cells as metabolite sensors and genetic regulators...
  49. pmc Subcellular location characteristics of the Pseudomonas aeruginosa GGDEF protein, WspR, indicate that it produces cyclic-di-GMP in response to growth on surfaces
    Zehra Tüzün Güvener
    Department of Microbiology, University of Washington, Seattle, WA 98195, USA
    Mol Microbiol 66:1459-73. 2007
    ..Our results suggest that the compartmentalized production of c-di-GMP in response to a stimulus associated with growth on a surface is an important functional characteristic of the Wsp system...
  50. ncbi Cyclic nucleotide research -- still expanding after half a century
    Joseph A Beavo
    Department of Pharmacology, University of Washington, Seattle, Washington 98195 7280, USA
    Nat Rev Mol Cell Biol 3:710-8. 2002
    ..cyclic AMP acts as a second messenger for the hormone adrenaline, interest in this molecule and its companion, cyclic GMP, has grown...
  51. ncbi HD-GYP domain proteins regulate biofilm formation and virulence in Pseudomonas aeruginosa
    Robert P Ryan
    Department of Microbiology, BIOMERIT Research Centre, Biosciences Institute, National University of Ireland, Cork, Ireland
    Environ Microbiol 11:1126-36. 2009
    ..Mutation of PA4108, PA4781 and PA2572 had distinct effects on biofilm formation and architecture of P. aeruginosa. All three proteins contributed to virulence of P. aeruginosa to larvae of the Greater Wax moth Galleria mellonella...
  52. ncbi A key role for cyclic nucleotide gated (CNG) channels in cGMP-related retinitis pigmentosa
    Francois Paquet-Durand
    Division of Experimental Ophthalmology, Institute for Ophthalmic Research, University of Tubingen, Röntgenweg 11, 72076 Tubingen, Germany
    Hum Mol Genet 20:941-7. 2011
    ..Furthermore, our findings suggest that targeting rod CNG channels, rather than general Ca(2+)-channel blockade, is a most promising symptomatic approach to treat otherwise incurable forms of cGMP-related RP...
  53. ncbi Multidestructive pathways triggered in photoreceptor cell death of the rd mouse as determined through gene expression profiling
    Baerbel Rohrer
    Departments of Ophthalmology, Medical University of South Carolina, Charleston, South Carolina 29425, USA
    J Biol Chem 279:41903-10. 2004
    ..All of these components need to be addressed if rods are to be successfully protected...
  54. ncbi Cyclic nucleotide phosphodiesterase (PDE) superfamily: a new target for the development of specific therapeutic agents
    Claire Lugnier
    CNRS UMR, 7034, Pharmacologie et Physicochimie des Interactions Moléculaires et Cellulaires, Faculte de Pharmacie, Universite Louis Pasteur de Strasbourg, 74 route du Rhin, BP 60024, 67401 Illkirch, France
    Pharmacol Ther 109:366-98. 2006
    ....
  55. pmc Identification of a novel regulatory protein (CsrD) that targets the global regulatory RNAs CsrB and CsrC for degradation by RNase E
    Kazushi Suzuki
    Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, Georgia 30322, USA
    Genes Dev 20:2605-17. 2006
    ..Thus, these studies demonstrate a novel process for the selective targeting of RNA molecules for degradation by RNase E and a novel function for a GGDEF-EAL protein...
  56. ncbi Allosteric control of cyclic di-GMP signaling
    Beat Christen
    Biozentrum, University of Basel, Switzerland
    J Biol Chem 281:32015-24. 2006
    ....
  57. ncbi Retinal degeneration in the rd mouse is caused by a defect in the beta subunit of rod cGMP-phosphodiesterase
    C Bowes
    Jules Stein Eye Institute, University of California, School of Medicine, Los Angeles 90024
    Nature 347:677-80. 1990
    ..Degeneration is preceded by accumulation of cyclic GMP in the retina and is correlated with deficient activity of the rod photoreceptor cGMP-phosphodiesterase...
  58. ncbi The cAMP receptor-like protein CLP is a novel c-di-GMP receptor linking cell-cell signaling to virulence gene expression in Xanthomonas campestris
    Ko Hsin Chin
    Institute of Biochemistry, National Chung Hsing University, Taichung 40227, Taiwan, ROC
    J Mol Biol 396:646-62. 2010
    ....
  59. ncbi Mutations in a cyclic nucleotide-gated channel lead to abnormal thermosensation and chemosensation in C. elegans
    H Komatsu
    Department of Biology, Faculty of Science, Kyushu University, Fukuoka, Japan
    Neuron 17:707-18. 1996
    ..The results suggest that a cyclic nucleotide-gated channel is required for thermosensation and chemosensation and that cGMP is an important intracellular messenger in C. elegans sensory transduction...
  60. pmc Targeting the heme-oxidized nitric oxide receptor for selective vasodilatation of diseased blood vessels
    Johannes Peter Stasch
    Institute of Cardiovascular Research, Bayer HealthCare, Wuppertal, Germany
    J Clin Invest 116:2552-61. 2006
    ROS are a risk factor of several cardiovascular disorders and interfere with NO/soluble guanylyl cyclase/cyclic GMP (NO/sGC/cGMP) signaling through scavenging of NO and formation of the strong oxidant peroxynitrite...
  61. pmc Cyclic di-GMP signaling in bacteria: recent advances and new puzzles
    Robert P Ryan
    BIOMERIT Research Centre, Department of Microbiology, Biosciences Institute, National University of Ireland, Cork, Ireland
    J Bacteriol 188:8327-34. 2006
  62. pmc Connecting quorum sensing, c-di-GMP, pel polysaccharide, and biofilm formation in Pseudomonas aeruginosa through tyrosine phosphatase TpbA (PA3885)
    Akihiro Ueda
    Artie McFerrin Department of Chemical Engineering, Texas A and M University, College Station, Texas, United States of America
    PLoS Pathog 5:e1000483. 2009
    ....
  63. pmc A cyclic GMP signalling module that regulates gliding motility in a malaria parasite
    Robert W Moon
    Wellcome Trust Sanger Institute, Cambridge Hinxton, United Kingdom
    PLoS Pathog 5:e1000599. 2009
    ..These data demonstrate an important function for signalling through cGMP, and most likely PKG, in dynamically regulating ookinete gliding during the transmission of malaria to the mosquito...
  64. pmc Structural insights into the regulatory mechanism of the response regulator RocR from Pseudomonas aeruginosa in cyclic Di-GMP signaling
    Ming wei Chen
    School of Biological Sciences, Nanyang Technological University, Singapore
    J Bacteriol 194:4837-46. 2012
    ....
  65. ncbi Structure of PP4397 reveals the molecular basis for different c-di-GMP binding modes by Pilz domain proteins
    Junsang Ko
    Department of Chemistry, KAIST, Gusong Dong 373 1, Yuseong Gu, Daejeon 305 701, South Korea
    J Mol Biol 398:97-110. 2010
    ..Thus, PilZ domain proteins exhibit different c-di-GMP binding stoichiometry and quaternary structure, and these differences are expected to play a role in generating diverse forms of c-di-GMP-mediated regulation...
  66. ncbi The response threshold of Salmonella PilZ domain proteins is determined by their binding affinities for c-di-GMP
    Ingrid Swanson Pultz
    Department of Microbiology, University of Washington, Seattle, WA 91895, USA
    Mol Microbiol 86:1424-40. 2012
    ....
  67. pmc Polymorphism of the signaling molecule c-di-GMP
    Zhaoying Zhang
    Department of Chemistry and Chemical Biology, Rutgers The State University of New Jersey, 610 Taylor Road, Piscataway, NJ 08854, USA
    J Am Chem Soc 128:7015-24. 2006
    ..The polymorphism we describe may provide different options for this signaling molecule when performing its functions in a bacterial cell, with K(+) and its own local concentration controlling the equilibrium...
  68. pmc The phytosulfokine (PSK) receptor is capable of guanylate cyclase activity and enabling cyclic GMP-dependent signaling in plants
    Lusisizwe Kwezi
    Medicinal Chemistry and Drug Action, Monash Institute of Pharmaceutical Sciences, Monash University Parkville Campus, 381 Royal Parade, Parkville, Victoria 3052, Australia
    J Biol Chem 286:22580-8. 2011
    ..Together these results indicate that the PSKR1 contains dual GC and kinase catalytic activities that operate in vivo and that this receptor constitutes a novel class of enzymes with overlapping catalytic domains...
  69. ncbi The bacterial second messenger c-di-GMP: mechanisms of signalling
    Erez Mills
    Department of Microbiology, University of Washington, Seattle, Washington 98195, USA
    Cell Microbiol 13:1122-9. 2011
    ..c-di-GMP specifically regulates multiple cellular processes by binding to diverse target molecules. This review highlights important questions in research into the mechanisms of c-di-GMP signalling and its role in bacterial physiology...
  70. ncbi Cyclic GMP controls Rhodospirillum centenum cyst development
    Jeremiah N Marden
    Indiana University, Bloomington, IN 47405, USA University of California at Berkeley, Berkeley, CA 94720, USA
    Mol Microbiol 79:600-15. 2011
    ....
  71. ncbi Cyclic GMP-dependent protein kinase signaling pathway inhibits RhoA-induced Ca2+ sensitization of contraction in vascular smooth muscle
    V Sauzeau
    Laboratoire de Physiologie Cellulaire et Moleculaire, INSERM U 533, Faculte des Sciences, 44322 Nantes, France
    J Biol Chem 275:21722-9. 2000
    The potent vasodilator action of cyclic GMP-dependent protein kinase (cGK) involves decreasing the Ca(2+) sensitivity of contraction of smooth muscle via stimulation of myosin light chain phosphatase through unknown mechanisms (Wu, X...
  72. ncbi The CRP/FNR family protein Bcam1349 is a c-di-GMP effector that regulates biofilm formation in the respiratory pathogen Burkholderia cenocepacia
    Mustafa Fazli
    Department of International Health, Immunology and Microbiology, Faculty of Health Sciences, University of Copenhagen, Copenhagen, Denmark
    Mol Microbiol 82:327-41. 2011
    ..Taken together, these findings suggest that the Bcam1349 protein is a transcriptional regulator that binds c-di-GMP and regulates biofilm formation and virulence in B. cenocepacia in response to the level of c-di-GMP...
  73. pmc The anti-aggregating effect of BAY 41-2272, a stimulator of soluble guanylyl cyclase, requires the presence of nitric oxide
    Séverine Roger
    Département Angiologie, Institut de Recherches Servier, 11 rue des Moulineaux, Suresnes, France
    Br J Pharmacol 161:1044-58. 2010
    ..The purpose of the present study was to determine whether a stimulator of soluble guanylyl cyclase, BAY 41-2272, inhibits platelet aggregation and to clarify its interaction with nitric oxide (NO)...
  74. ncbi Cyclic-di-GMP-mediated signalling within the sigma network of Escherichia coli
    Harald Weber
    Institut fur Biologie, Mikrobiologie, Freie Universitat Berlin, 14195 Berlin, Germany
    Mol Microbiol 62:1014-34. 2006
    ....
  75. pmc Cyclic diguanylate (c-di-GMP) regulates Vibrio cholerae biofilm formation
    Anna D Tischler
    Department of Molecular Biology and Microbiology, Tufts University School of Medicine, 136 Harrison Avenue, Boston, MA 02111, USA
    Mol Microbiol 53:857-69. 2004
    ..We propose that c-di-GMP synthetase and phosphodiesterase domain-containing proteins contribute to regulating biofilm formation by controlling c-di-GMP concentration...
  76. pmc DgrA is a member of a new family of cyclic diguanosine monophosphate receptors and controls flagellar motor function in Caulobacter crescentus
    Matthias Christen
    Biozentrum, University of Basel, Klingelbergstrasse 70, CH 4056 Basel, Switzerland
    Proc Natl Acad Sci U S A 104:4112-7. 2007
    ..crescentus by interfering with motor function rather than flagellar assembly. We present preliminary evidence implicating the flagellar motor protein FliL in DgrA-dependent cell motility control...
  77. pmc BifA, a cyclic-Di-GMP phosphodiesterase, inversely regulates biofilm formation and swarming motility by Pseudomonas aeruginosa PA14
    Sherry L Kuchma
    Department of Microbiology and Immunology, Dartmouth Medical School, Rm 505, Vail Building, North College St, Hanover, NH 03755, USA
    J Bacteriol 189:8165-78. 2007
    ..The DeltabifA mutation also results in decreased flagellar reversals. Based on epistasis studies with the previously described sadB gene, we propose that BifA functions upstream of SadB in the control of biofilm formation and swarming...
  78. pmc Quorum sensing controls biofilm formation in Vibrio cholerae through modulation of cyclic di-GMP levels and repression of vpsT
    Christopher M Waters
    Department of Molecular Biology, Lewis Sigler Institute for Integrative Genomics, Princeton University, Princeton, NJ 08544, USA
    J Bacteriol 190:2527-36. 2008
    ..cholerae integrates information about the vicinal bacterial community contained in extracellular QS autoinducers with the intracellular environmental information encoded in c-di-GMP to control biofilm formation...
  79. pmc Structural analysis of the GGDEF-EAL domain-containing c-di-GMP receptor FimX
    Marcos V A S Navarro
    Department of Molecular Medicine, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA
    Structure 17:1104-16. 2009
    ..The results provide the structural basis for c-di-GMP sensing via degenerate phosphodiesterases...
  80. pmc The cyclic nucleotide monophosphate domain of Xanthomonas campestris global regulator Clp defines a new class of cyclic di-GMP effectors
    Fei Tao
    Institute of Molecular and Cell Biology, Singapore, Singapore
    J Bacteriol 192:1020-9. 2010
    ..These data establish the direct role of Clp in the response to fluctuating c-di-GMP levels and depict a novel mechanism by which QS links the second messenger with the X. campestris pv. campestris virulence regulon...
  81. ncbi Involvement of cyclic GMP and protein kinase G in the regulation of apoptosis and survival in neural cells
    Ronald R Fiscus
    Department of Physiology, Faculty of Medicine, Epithelial Cell Biology Research Center, Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China
    Neurosignals 11:175-90. 2002
    Our current understanding of nitric oxide (NO), cyclic GMP (cGMP) and protein kinase G (PKG) signaling pathways in the nervous systems has its origins in the early studies conducted on vascular tissues during the late 1970s and early to ..
  82. ncbi Intestinal secretory defects and dwarfism in mice lacking cGMP-dependent protein kinase II
    A Pfeifer
    Institut f ur Pharmakologie und Toxikologie, Technische Universitat Munchen, Biedersteiner Strasse 29, D 80802 M unchen, Germany
    Science 274:2082-6. 1996
    ..The cGKII-deficient mice also developed dwarfism that was caused by a severe defect in endochondral ossification at the growth plates. These results indicate that cGKII plays a central role in diverse physiological processes...
  83. ncbi Cyclic di-GMP activation of polynucleotide phosphorylase signal-dependent RNA processing
    Jason R Tuckerman
    Department of Biochemistry, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390 9038, USA
    J Mol Biol 407:633-9. 2011
    ..We conclude that c-di-GMP can mediate signal-dependent RNA processing and that macromolecular complexes can compartmentalize c-di-GMP signaling...
  84. pmc How vision begins: an odyssey
    Dong Gen Luo
    Solomon H Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Proc Natl Acad Sci U S A 105:9855-62. 2008
    ..It then highlights some details discovered more recently, followed by a comparison between rods and cones...
  85. ncbi Nitric oxide-independent stimulation of soluble guanylate cyclase with BAY 41-2272 in cardiovascular disease
    Guido Boerrigter
    Cardiorenal Research Laboratory, Mayo Clinic, Mayo Clinic College of Medicine, Rochester, MN 55905, USA
    Cardiovasc Drug Rev 25:30-45. 2007
    ..Finally, the significance of the novel NO- and heme-independent sGC activator BAY 58-2667, which activates two forms of NO-insensitive sGC, is briefly discussed...
  86. pmc Structural basis for c-di-GMP-mediated inside-out signaling controlling periplasmic proteolysis
    Marcos V A S Navarro
    Department of Molecular Medicine, College of Veterinary Medicine, Cornell University, Ithaca, New York, United States of America
    PLoS Biol 9:e1000588. 2011
    ..Our structural and functional analyses identified a conserved system for the regulation of periplasmic proteases in a wide variety of bacteria, including many free-living and pathogenic species...
  87. pmc Nitric oxide modulates bacterial biofilm formation through a multicomponent cyclic-di-GMP signaling network
    Lars Plate
    Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA
    Mol Cell 46:449-60. 2012
    ..Cellular adhesion may provide a protection mechanism for bacteria against reactive and damaging NO. These results are broadly applicable to H-NOX-mediated NO signaling in bacteria...
  88. ncbi cGMP signaling in vertebrate retinal photoreceptor cells
    Xiujun Zhang
    Department of Biochemistry and Molecular Biology, University of New Hampshire, Durham, NH 03824 2617, USA
    Front Biosci 10:1191-204. 2005
    ....
  89. ncbi Nitric oxide blocks hKv1.5 channels by S-nitrosylation and by a cyclic GMP-dependent mechanism
    Lucía Núñez
    Department of Pharmacology, School of Medicine, Universidad Complutense, 28040 Madrid, Spain
    Cardiovasc Res 72:80-9. 2006
    ..5, which generates the ultrarapid delayed rectifier current (IKur) that determines the height and duration of atrial action potentials...
  90. pmc Cyclic GMP and protein kinase G control a Src-containing mechanosome in osteoblasts
    Hema Rangaswami
    Department of Medicine, University of California, San Diego, La Jolla, CA 92093, USA
    Sci Signal 3:ra91. 2010
    ..These results support the use of PKG-activating drugs to mimic the anabolic effects of mechanical stimulation of bone in the treatment of osteoporosis...
  91. ncbi Shattuck Lecture. Nitric oxide and cyclic GMP in cell signaling and drug development
    Ferid Murad
    Brown Institute of Molecular Medicine, University of Texas Health Science Center, Houston, USA
    N Engl J Med 355:2003-11. 2006
  92. ncbi cGMP-dependent ABA-induced stomatal closure in the ABA-insensitive Arabidopsis mutant abi1-1
    Lyudmila V Dubovskaya
    National Academy of Sciences of Belarus, Institute of Biophysics and Cell Engineering, Minsk, Belarus
    New Phytol 191:57-69. 2011
    ..This study positions cGMP downstream of ABA-induced changes in H(2) O(2) and NO, and upstream of increases in [Ca(2+) ](cyt) in the signalling pathway leading to stomatal closure...
  93. pmc Defective trafficking of cone photoreceptor CNG channels induces the unfolded protein response and ER-stress-associated cell death
    Deborah L Duricka
    Department of Veterinary and Comparative Anatomy, Pharmacology and Physiology, Washington State University, Pullman, WA 99164, USA
    Biochem J 441:685-96. 2012
    ..These results indicate that ER stress can arise from expression of localization-defective CNG channels, and may represent a contributing factor for photoreceptor degeneration...
  94. pmc Structural and biochemical determinants of ligand binding by the c-di-GMP riboswitch
    Kathryn D Smith
    Department of Chemistry, Yale University, New Haven, Connecticut 06520, USA
    Biochemistry 49:7351-9. 2010
    ..Many mutants retain sufficient c-di-GMP affinity for the RNA to remain biologically relevant, which suggests that this motif is quite resilient to mutation...
  95. ncbi Nitric oxide synthase and cyclic GMP signaling in cardiac myocytes: from contractility to remodeling
    Joanna Hammond
    Pole of Pharmacology and Therapeutics FATH, Institut de Recherche Expérimentale et Clinique IREC, Universite Catholique de Louvain UCL, 52 Avenue Mounier, 1200 Brussels, EU, Belgium
    J Mol Cell Cardiol 52:330-40. 2012
    ..Thus, enhancing cardiac myocyte cGMP signaling represents a promising therapeutic target for treatment of cardiovascular disease. This article is part of a Special Issue entitled "Local Signaling in Myocytes."..
  96. pmc Differential radial capillary action of ligand assay for high-throughput detection of protein-metabolite interactions
    Kevin G Roelofs
    Department of Cell Biology and Molecular Genetics, and Maryland Pathogen Research Institute, University of Maryland, College Park, MD 20742, USA
    Proc Natl Acad Sci U S A 108:15528-33. 2011
    ..The ease of obtaining metabolite-protein interaction data using the DRaCALA assay will facilitate rapid identification of protein-metabolite and protein-pharmaceutical interactions in a systematic and comprehensive approach...
  97. ncbi An oxygen-sensing diguanylate cyclase and phosphodiesterase couple for c-di-GMP control
    Jason R Tuckerman
    Department of Biochemistry, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, Texas 75390 9038, USA
    Biochemistry 48:9764-74. 2009
    ..Finally, we find that DosP and DosC associate into a functional complex. We conclude that the dosCP operon encodes two oxygen sensors that cooperate in the controlled production and removal of c-di-GMP...
  98. ncbi Taming platelets with cyclic nucleotides
    U R Schwarz
    , , Josef-Schneider Str. 2, 97080, , Germany
    Biochem Pharmacol 62:1153-61. 2001
    ..function by activating adenylyl and guanylyl cyclases and increasing intracellular cyclic AMP (cAMP) and cyclic GMP (cGMP) levels, respectively...
  99. ncbi Characterization of starvation-induced dispersion in Pseudomonas putida biofilms: genetic elements and molecular mechanisms
    Morten Gjermansen
    Department of International Health, Immunology and Microbiology, Faculty of Health Sciences, University of Copenhagen, Copenhagen, Denmark
    Mol Microbiol 75:815-26. 2010
    ..In addition, evidence is provided that associated to LapA a cellulase-degradable exopolysaccharide is part of the P. putida biofilm matrix...
  100. pmc Cyclic di-GMP signaling regulates invasion by Ehrlichia chaffeensis of human monocytes
    Yumi Kumagai
    Department of Veterinary Biosciences, The Ohio State University, Columbus, OH 43210, USA
    J Bacteriol 192:4122-33. 2010
    ..Furthermore, anti-HtrA inhibited CDGA-induced TRP120 degradation. Our results suggest that E. chaffeensis invasion is regulated by c-di-GMP signaling, which stabilizes some bacterial surface-exposed proteins against proteases...
  101. pmc Role of cyclic Di-GMP during el tor biotype Vibrio cholerae infection: characterization of the in vivo-induced cyclic Di-GMP phosphodiesterase CdpA
    Rita Tamayo
    Howard Hughes Medical Institute and the Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, MA 02111, USA
    Infect Immun 76:1617-27. 2008
    ..cholerae life cycle in which c-di-GMP must be down-regulated early after entering the small intestine and maintained at a low level to allow virulence gene expression, colonization, and motility at appropriate stages of infection...

Research Grants63

  1. L-arginine supplement for the prevention of calcineurin inhibitor nephrotoxicity
    W Ray Kim; Fiscal Year: 2011
    ..Once established, there is no effective treatment. This research investigates whether L- arginine can reverse the effects of calcineurin inhibitors on the kidneys and thus prevent renal insufficiency in liver transplant recipients. ..
  2. Anti-CD47 mAb Therapy to Improve Kidney Transplantation
    PAMELA ANN TOY-MANNING; Fiscal Year: 2011
    ..b>Cyclic GMP levels in kidneys and plasma nitrite levels will be determined to confirm the mechanism of anti-CD47 mAb action ..
  3. Intrauterine hypoxia induces cytokine gene expression in fetal cardiac ventricles
    LASHAUNA C EVANS; Fiscal Year: 2010
    ..Further, proinflammatory cytokine release has been shown to be stimulated by cyclic GMP. Therefore we hypothesize that chronic hypoxia increases proinflammatory cytokine production in fetal hearts via ..
  4. Multifactor Risk Reduction for Optimal Management in Peripheral Arterial Disease
    Roberta K Oka; Fiscal Year: 2010
    ..by urine nitrogen oxide, plasma nitrogen oxide, plasma asymmetric dimethylarginine, plasma, urine and platelet cyclic GMP)...
  5. PRESYNAPTIC LONG-TERM SYNAPTIC DEPRESSION
    Patric K Stanton; Fiscal Year: 2012
    ..distinct cascades, one dependent on the actions of the intercellular messenger nitric oxide (NO), production of cyclic GMP and inhibition of cyclic AMP-dependent protein kinase, which acts on presynaptic terminals to persistently ..
  6. Satiety signaling in Caenorhabditis elegans
    Leon Avery; Fiscal Year: 2013
    ..4. Satiety hormones act on downstream neurons to change behavior. 5. Cyclic GMP (cGMP) activates cGMP-dependent protein kinase (PKG) in ASI and in downstream neurons to produce satiety. Aim 1...
  7. PKG Redox Modulation of Cardiac Function and Disease
    DAVID ALAN KASS; Fiscal Year: 2013
    ..The cyclic GMP-protein kinase G1a (PKG1a) pathway is a prime example of the latter...
  8. Expression of PKG in Vascular Smooth Muscle Cells
    Thomas M Lincoln; Fiscal Year: 2013
    ..b>Cyclic GMP activates a serine/threonine protein kinase, PKG-I, in vascular smooth muscle that leads to increases in normal ..
  9. Mechanisms regulating PKGI proteolysis and modulation of pulmonary SMC phenotype
    Jesse D Roberts; Fiscal Year: 2013
    ..b>Cyclic GMP modulates PASMC phenotype primarily by stimulating cGMP-dependent protein kinase I (PKGI)...
  10. Regulation and function of cGMP in the nervous system
    David B Morton; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): The intracellular messenger, cyclic GMP (cGMP) has diverse physiological roles in a variety of tissues and there is a correspondingly diverse array of human diseases and disorders resulting from ..
  11. Role of cGKII in AMPA Receptor Transport
    Edward B Ziff; Fiscal Year: 2013
    ..and electrophysiological approaches to investigate a new mechanism of serine 845 phosphorylation by the cyclic GMP regulated kinase cGKII...
  12. REGULATION OF CYCLIC GMP SYNTHESIS IN PHOTORECEPTORS
    Alexander M Dizhoor; Fiscal Year: 2013
    ..By completing these specific aims, we expect to overcome some critical barriers in understanding of RetGC regulation and its role in normal photoreceptor physiology and in retinal diseases. ..
  13. iPSC-derived cardiomyocytes to study dystrophic cardiomyopathy
    JENNIFER LYNN STRANDE; Fiscal Year: 2013
    ..cardiomyocytes containing different dystrophin mutations and 3) determine whether up-regulating the nitric oxide-cyclic GMP pathway rescues MD- iPSCs from oxidative stress and cell death...
  14. CGMP AND PHOTORECEPTOR FUNCTION
    Rick H Cote; Fiscal Year: 2012
    b>Cyclic GMP (cGMP) is the primary intracellular messenger for the visual signaling pathway in rod and cone photoreceptor cells of the retina...
  15. Regulation of Guanylyl Cyclases in The Olfacory System
    Gabriele V Ronnett; Fiscal Year: 2012
    ..We demonstrated that cyclic GMP (cGMP) plays a role in the proliferation, differentiation, synapse formation, and neuroprotection of OSNs...
  16. UCSF Clinical Research Center for Prematurity and Respiratory Outcomes Program
    Roberta L Keller; Fiscal Year: 2013
    ..noninvasive collections of urine during the evolution of lung disease to evaluate production of nitric oxide/cyclic GMP and turnover of pulmonary elastin...
  17. Human Cardiorenal Syndrome
    Horng H Chen; Fiscal Year: 2013
    ..b>Cyclic GMP (cGMP) is the second messenger of both the natriuretic peptide (NP) system and the nitric oxide system (NO)...
  18. Control of Photoreceptor Metabolism
    JAMES BRYANT HURLEY; Fiscal Year: 2013
    ..b>Cyclic GMP and Ca2+ play central roles in the way photoreceptors respond and adapt to light...
  19. ACTIONS OF CYCLIC GMP IN SMOOTH MUSCLE
    Thomas Lincoln; Fiscal Year: 1999
    b>Cyclic GMP mediates vascular smooth muscle relaxation to nitric oxide and atrial natriuretic peptides...
  20. Functional Interaction of ANP and NO signaling
    Kumar U Kotlo; Fiscal Year: 2010
    ..Both ANP and NO increase intracellular cyclic GMP (cGMP) levels thus leading to reduction in arterial blood pressure and inhibition of renal tubular sodium ..
  21. NEUROTRANSMITTER TRANSPORT
    GARY W RUDNICK; Fiscal Year: 2013
    ..This mutant is apparently activated in a constitutive fashion by a pathway normally involving cyclic GMP (cGMP)...
  22. Role of Nitric Oxide and Cyclic GMP in Stem Cells
    David G Gorenstein; Fiscal Year: 2010
    In this revised application, we will primarily focus on studying the role of NO and cyclic GMP signaling in proliferation and differentiation of human and mouse embryonic stem cells into cardiomyocytes...
  23. REGULATION OF CYCLIC GMP DEPENDENT PROTEIN KINASE
    MICHAEL UHLER; Fiscal Year: 1999
    The second messenger, cyclic GMP (cGMP) is believed to mediate the intracellular effects of nitric oxide and atrial natriuretic factor (ANF) on the relaxation of smooth muscle...
  24. Structure and Regulation of the GPCR-G Protein Interactions in the Visual System
    ARNOLD EINO RUOHO; Fiscal Year: 2011
    ..2) To identify the interaction surfaces between the PDE6 gamma subunit (PDEg), cyclic GMP and the PDE6 catalytic heterodimer subunits (PDEg)...
  25. CGMP PROTEIN KINASE IN PREGNANT MYOMETRIUM
    Trudy Cornwell; Fiscal Year: 2000
    The long-term goals of this project are to understand the role of the cyclic GMP (cGMP)-dependent protein kinase (cG-kinase) in the normal uterus and how cGMP signaling through this enzyme is modified by progesterone...
  26. Cyclic GMP Phosphodiesterase in Signaling
    Hsien Yu Wang; Fiscal Year: 2007
    ..G-protein subunits G(alpha)t2/o in mouse F9 teratocarcinoma stem cells and treatment with inhibitors of cyclic GMP PDE, such as IBMX, zaprinast, and dipyridamole, block the ability of the GPCR rat Fz2 to signal at the level of ..
  27. CGMP DEPENDENT PROTEIN KINASE IN VASCULAR BIOLOGY
    HOWARD SURKS; Fiscal Year: 2003
    ..b>Cyclic GMP-dependent protein kinase (cGK) mediates the physiologic relaxation of vascular smooth muscle in response to ..
  28. CYCLIC GMP REGULATION OF SMOOTH MUSCLE CONTRACTILITY
    Robert Rapoport; Fiscal Year: 1990
    ..There is considerable evidence that cyclic GMP exerts an important fundamental influence on regulation...
  29. RAT MODEL OF FETAL ALCOHOL SYNDROME
    ASA BLACK; Fiscal Year: 1993
    ..Stimlation of these muscarinic cholinergic receptors causes increases in cyclic GMP, a seoond messenger involved in synaptic transmission...
  30. ATRIAL NATRIURETIC PEPTIDE IN RENAL GROWTH
    ROBERT CHEVALIER; Fiscal Year: 1990
    ..rat ANP infusion and to acute volume expansion will be determined by assay of plasma ANP, and plasma and urinary cyclic GMP, which are stimulated by circulating ANP...
  31. cGMP Phosphodiesterase, a Novel Chemoprevention Target
    Gary Piazza; Fiscal Year: 2005
    The overall objective of this proposal is to validate cyclic GMP phosphodiesterase (cGMP PDE) as a novel drug target for cancer chemoprevention...
  32. A Novel HTS Cell-Based Assay For Cyclic GMP
    Gary Piazza; Fiscal Year: 2007
    ..Towards this goal, we propose to develop an innovative cell-based assay involving a fluorescence cGMP biosensor for high throughput screening. [unreadable]..
  33. CONFORMATIONAL ASPECTS OF VISUAL EXCITATION
    LUBERT STRYER; Fiscal Year: 1993
    ..light-triggered amplification cycle in rod outer segments involving photoexcited rhodopsin, transducin, and the cyclic GMP phosphodiesterase (PDE) will be investigated in molecular detail...
  34. SIGNALING PATHWAY FOR BENZODIAZEPINE INDUCED BEHAVIORS
    RAYMOND QUOCK; Fiscal Year: 1999
    ..action at the benzodiazepine/y aminobutyric acidA (GABAA) receptor complex are mediated by a nitric oxide (NO)/cyclic GMP (cGMP)/protein kinase G (PKG) signaling pathway, i.e...
  35. Nitric Oxide Regulation of Adrenal Steroidogenesis
    William Campbell; Fiscal Year: 2004
    ..The inhibition by NO is not mediated by cyclic GMP but by NO binding to the cytochrome P450 enzymes involved in aldosterone biosynthesis...
  36. OPSIN CGMP-ACTIVATED SINGLE CHANNEL ACTIVITY
    JAMES CLACK; Fiscal Year: 1991
    The goal of this project is determine whether vertebrate opsin or rhodopsin can function as a cyclic GMP-activated channel Opsin will be purified, reconstituted into phospholipid vesicles, and single channel activity will be recorded from ..
  37. MECHANISMS OF ANGIOTENSIN II: CYCLIC NUCLEOTIDES & CA2+
    LARRY BARNES; Fiscal Year: 1980
    ..Glomerular concentrations of cyclic AMP and cyclic GMP, determined by radioimmunoassays, will be analyzed as a function of binding of A II and glomerular contractility...
  38. FEEDBACK REGULATION OF THE RETINAL ROD LIGHT RESPONSE
    Ronald Brown; Fiscal Year: 1999
    In retinal rods, incident light triggers a highly amplified enzymatic cascade that results in the closure of cyclic GMP-activated channels in the plasma membrane. The gain of the phototransduction cascade is set by the ambient light level...
  39. Cyclic GMP And Ventricular Function In Heart Failure
    MARGARET REDFIELD; Fiscal Year: 2003
    ..abstract_text> ..
  40. Effects of Insulin on VSM Migration
    Andrew Kahn; Fiscal Year: 2004
    ..We will test the overall hypothesis that insulin acts synergistically with NO to stimulate cyclic GMP production which inhibits VSMC migration in-vitro, and test whether insulin inhibits VSMC migration in-vivo ..
  41. REGULATION OF THE ANP RECEPTOR/GUANYLYL CYCLASE
    Michael Chinkers; Fiscal Year: 2000
    ..broad long-term objectives of this proposal are to understand the mechanisms involved in signaling through the cyclic GMP pathway, the normal biological role of PP5, a protein-serine phosphatase that is phosphorylated and activated by ..
  42. RECEPTOR INTERACTIONS IN CILIARY PROCESSES
    LARRY BAUSHER; Fiscal Year: 1993
    ..Most of the in vitro experiments proposed will explore cyclic AMP and cyclic GMP production by intact, excised rabbit ciliary processes; however, where significant results are obtained in this ..
  43. OXYGEN TENSION AND ENDOTHELIUM-DEPENDENT VASODILATION
    Roger Johns; Fiscal Year: 1992
    ..biologic half-life; and its relaxing action on vascular smooth muscle correlates with an increase in muscle cyclic GMP concentration...
  44. ENDOTHELIAL-DERIVED RELAXING FACTOR & PLATELET FUNCTION
    Michael Mendelsohn; Fiscal Year: 1993
    ..EDRF and known endothelial-derived inhibitors of platelet function, (3) the role of S-nitrosothiols and the cyclic GMP pathway in the inhibition of platelet aggregation by EDRF, and (4) interactions of fibrinogen with its platelet ..
  45. INHIBITION OF VASCULAR CELL PROLIFERATION BY CYCLIC GMP
    Aviv Hassid; Fiscal Year: 2000
    ..To this end, the PI proposes to test the following hypothesis: Can cyclic GMP agonists and other agents that decrease cytoplasmic calcium levels activate a specific PTPase that is inhibited ..
  46. TEMPORAL VULNERABILITY OF DEVELOPING RAT BRAIN TO ETOH
    ASA BLACK; Fiscal Year: 1993
    ..Stimulation of these muscarinic cholinergic receptors causes increases in cyclic GMP, a second messenger involved in synaptic transmission in the septo-hippocampal pathway...
  47. NITRIC OXIDE AND IN VIVO BRAIN ARTERIOLAR RELAXATION
    Dale Pelligrino; Fiscal Year: 2004
    ..will address control mechanisms occurring chiefly in cerebral vascular smooth muscle (VSM) at the level of cyclic GMP and cyclic AMP...
  48. MECHANISMS OF ESTROGEN INDUCED CORONARY VASODILATION
    Richard White; Fiscal Year: 2000
    ..that could mediate estrogen-induced relaxation of CSM: stimulation of BK(Ca) potassium channel gating through cyclic GMP-dependent phosphorylation...
  49. REGULATION OF CYCLIC NUCLEOTIDE FORMATION AND ACTION
    Ferid Murad; Fiscal Year: 1991
    ..the mechanisms of hormone, autocoid and drug regulation of the isoenzyme forms of guanylate cyclase and cyclic GMP synthesis...
  50. MYOCARDIAL AGING AND NITRIC OXIDE
    Harvey Weiss; Fiscal Year: 1999
    ..It acts through the production of the second messenger cyclic GMP. During heart failure, cyclic GMP's "braking" effects are reduced although its level increases...
  51. Adrenergic Receptor Linked ROS-GC Neurosensory Signaling
    Rameshwar Sharma; Fiscal Year: 2006
    ..Acceleration causes an increment, and inhibition causes a decrement in the production of the second messenger cyclic GMP. It is then predicted that cyclic GMP, via a cyclic GMP-gated channel, regulates the degree of polarization of ..
  52. CYCLIC GMP-REGULATED ION CHANNELS IN VASCULAR CELLS
    Yan Zhu; Fiscal Year: 2000
    ..Nitric oxide and related nitrovasodilators act by stimulating guanylate cyclase and elevating cyclic GMP (cGMP), but the mechanism by which increased cGMP leads to vasodilation is not fully understood...
  53. PHOSPHODIESTERASES IN PHOTORECEPTOR METABOLISM & DISEASE
    Debora Farber; Fiscal Year: 2003
    ..the molecular events that control transcription and translation of the genes encoding rod- and cone-specific cyclic GMP phosphodiesterases (PDEs), enzymes that are fundamental for integrity and normal function of photoreceptors, and ..
  54. ROLES OF CGMP-GATED CHANNELS IN RETINAL BIPOLAR NEURONS
    Gary Matthews; Fiscal Year: 2005
    ..These cation channels may be members of the family of CNG channels, which open when cyclic GMP binds directly to the intracellular portion of the channel...
  55. The Rac-cGMP Signaling Pathway
    Xin Yun Huang; Fiscal Year: 2012
    ..Many membrane-signaling receptors can increase the cellular levels of the ubiquitous second messenger cyclic GMP (cGMP)...
  56. ATRIOPEPTIN-EVOKED CALCIUM AND LIPID METABOLISM
    Aviv Hassid; Fiscal Year: 1991
    ..is as follows: APs decrease both resting and vasoconstrictor-elevated cytosolic free Ca by increasing cellular cyclic GMP levels...
  57. CYCLIC GMP AND KB-DEPENDENT TRANSCRIPTION IN NEURONS
    STEVEN BARGER; Fiscal Year: 2000
    DESCRIPTION: Cyclic GMP (cGMP) has been characterized as a critical, pluripotent second messenger in peripheral tissues...
  58. MOLECULAR DETERMINANTS OF VISUAL CORTICAL PLASTICITY
    Colin Barnstable; Fiscal Year: 2004
    DESCRIPTION: Cyclic GMP is a vital second messenger in the CNS. It has been shown to have specific actions at presynaptic and postsynaptic sites that induce dramatic short-term alterations in synaptic efficacy...
  59. Photoreceptor Membrane Guanylyl Cyclases
    James Hurley; Fiscal Year: 2006
    ..A cyclic GMP-mediated enzymatic pathway is used to detect light in rod and cone photoreceptors...
  60. Molecular Mechanisms of PDE5 Regulation
    Jackie Corbin; Fiscal Year: 2005
    ..Results of these studies will provide a basis for understanding fundamental questions relating to cGMP signaling in many tissues. ..
  61. Molecular Control of cGMP Signaling by PKGs and PDEs
    Jackie Corbin; Fiscal Year: 2007
    ..A thorough physical and biochemical characterization of PDE11 will be carded out, and the likelihood of regulation of this enzyme by ligand binding to its GAF domains or by enzyme phosphorylation will be explored. ..
  62. REACTIVE OXYGEN SPECIES AND VASCULAR O2 SENSING
    Michael S Wolin; Fiscal Year: 2010
    ..These studies should help define the origins of differences in physiological responses of pulmonary and coronary arteries to changes in O2 tension. ..
  63. PULMONARY VASODILATION AND GUANYLATE CYCLASE REGULATION
    Michael S Wolin; Fiscal Year: 2010
    ....