guanosine

Summary

Summary: A purine nucleoside that has guanine linked by its N9 nitrogen to the C1 carbon of ribose. It is a component of ribonucleic acid and its nucleotides play important roles in metabolism. (From Dorland, 28th ed)

Top Publications

  1. doi Quantitative analysis of RNA solvent accessibility by N-silylation of guanosine
    Stefanie A Mortimer
    Department of Chemistry, University of North Carolina, Chapel Hill, North Carolina 27599 3290, USA
    Biochemistry 48:2109-14. 2009
  2. ncbi Repair of oxidative DNA damage: mechanisms and functions
    A L Lu
    Department of Biochemistry and Molecular Biology, School of Medicine, University of Maryland, Baltimore 21201, USA
    Cell Biochem Biophys 35:141-70. 2001
  3. doi Guanosine is neuroprotective against oxygen/glucose deprivation in hippocampal slices via large conductance Ca²+-activated K+ channels, phosphatidilinositol-3 kinase/protein kinase B pathway activation and glutamate uptake
    T Dal-Cim
    Departamento de Bioquimica, Centro de Ciencias Biologicas, Universidade Federal de Santa Catarina, Trindade, 88040 900 Florianopolis, SC, Brazil
    Neuroscience 183:212-20. 2011
  4. doi Guanosine protects human neuroblastoma SH-SY5Y cells against mitochondrial oxidative stress by inducing heme oxigenase-1 via PI3K/Akt/GSK-3β pathway
    Tharine Dal-Cim
    Departamento de Bioquimica, Centro de Ciencias Biologicas, Universidade Federal de Santa Catarina, Trindade, 88040 900 Florianopolis, SC, Brazil
    Neurochem Int 61:397-404. 2012
  5. pmc Solution structure of a unique G-quadruplex scaffold adopted by a guanosine-rich human intronic sequence
    Vitaly Kuryavyi
    Structural Biology Program, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA
    Structure 18:73-82. 2010
  6. pmc OGG1 initiates age-dependent CAG trinucleotide expansion in somatic cells
    Irina V Kovtun
    Department of Pharmacology and Experimental Therapeutics, Mayo Clinic and Foundation, 200 First Street SW, Rochester, Minnesota 55905, USA
    Nature 447:447-52. 2007
  7. pmc The nuclear cap-binding complex interacts with the U4/U6·U5 tri-snRNP and promotes spliceosome assembly in mammalian cells
    Marta Pabis
    Max Planck Institute of Molecular Cell Biology and Genetics, 01307 Dresden, Germany
    RNA 19:1054-63. 2013
  8. pmc Bacillus subtilis RNA deprotection enzyme RppH recognizes guanosine in the second position of its substrates
    Jérémie Piton
    Centre National de la Recherche Scientifique CNRS, Unité Propre de Recherche 9073 affiliated with Université Paris Diderot, Sorbonne Paris Cité Institut de Biologie Physico Chimique, 75005 Paris, France
    Proc Natl Acad Sci U S A 110:8858-63. 2013
  9. doi Electrochemical simulation of oxidation processes involving nucleic acids monitored with electrospray ionization-mass spectrometry
    Florian Pitterl
    Institute of Legal Medicine, Innsbruck Medical University, Muellerstrasse 44, 6020 Innsbruck, Austria
    Anal Bioanal Chem 397:1203-15. 2010
  10. pmc Pumilio 2 controls translation by competing with eIF4E for 7-methyl guanosine cap recognition
    Quiping Cao
    Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
    RNA 16:221-7. 2010

Research Grants

  1. DNA methylation of extracellular superoxide dismutase in pulmonary hypertension
    EVA NOZIK GRAYCK; Fiscal Year: 2012
  2. Rac GTPase signaling during embryonic salivary gland branching morphogenesis
    Sharon Sequeira; Fiscal Year: 2012
  3. Peter Sarnow; Fiscal Year: 2015
  4. Prevention of Inherited Retinal Diseases by Therapeutic Rare Earth Nanoparticles
    JAMES FRANCIS MCGINNIS; Fiscal Year: 2010
  5. Biomarkers to Assess Selenium Chemoprevention for NSCLC
    Steven A Belinsky; Fiscal Year: 2012
  6. High-affinity RNA targets of Survival Motor Neuron Protein
    Ravindra N Singh; Fiscal Year: 2013
  7. Carl W White; Fiscal Year: 2015
  8. Peptide Therapy for Pulmonary Arterial Hypertension
    Jawaharlal M Patel; Fiscal Year: 2013
  9. Nancy J Brown; Fiscal Year: 2014
  10. Peter A Beal; Fiscal Year: 2016

Detail Information

Publications347 found, 100 shown here

  1. doi Quantitative analysis of RNA solvent accessibility by N-silylation of guanosine
    Stefanie A Mortimer
    Department of Chemistry, University of North Carolina, Chapel Hill, North Carolina 27599 3290, USA
    Biochemistry 48:2109-14. 2009
    ..We show that N,N-(dimethylamino)dimethylchlorosilane (DMAS-Cl) reacts at the guanosine N2 position to yield a near-perfect measure (r >or= 0...
  2. ncbi Repair of oxidative DNA damage: mechanisms and functions
    A L Lu
    Department of Biochemistry and Molecular Biology, School of Medicine, University of Maryland, Baltimore 21201, USA
    Cell Biochem Biophys 35:141-70. 2001
    ..It is possible that the successive players in the repair pathway are assembled in a complex to perform concerted actions. The BER pathways are proposed to protect cells and organisms from mutagenesis and carcinogenesis...
  3. doi Guanosine is neuroprotective against oxygen/glucose deprivation in hippocampal slices via large conductance Ca²+-activated K+ channels, phosphatidilinositol-3 kinase/protein kinase B pathway activation and glutamate uptake
    T Dal-Cim
    Departamento de Bioquimica, Centro de Ciencias Biologicas, Universidade Federal de Santa Catarina, Trindade, 88040 900 Florianopolis, SC, Brazil
    Neuroscience 183:212-20. 2011
    ..kinase (PI3K) pathway involvement in the mechanisms related to the neuroprotective role of guanosine in rat hippocampal slices subjected to OGD...
  4. doi Guanosine protects human neuroblastoma SH-SY5Y cells against mitochondrial oxidative stress by inducing heme oxigenase-1 via PI3K/Akt/GSK-3β pathway
    Tharine Dal-Cim
    Departamento de Bioquimica, Centro de Ciencias Biologicas, Universidade Federal de Santa Catarina, Trindade, 88040 900 Florianopolis, SC, Brazil
    Neurochem Int 61:397-404. 2012
    ..Here we have investigated the protective mechanism of action of guanosine, the guanine nucleoside, in a human neuroblastoma cell line, SH-SY5Y, subjected to mitochondrial oxidative ..
  5. pmc Solution structure of a unique G-quadruplex scaffold adopted by a guanosine-rich human intronic sequence
    Vitaly Kuryavyi
    Structural Biology Program, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA
    Structure 18:73-82. 2010
    We report on the solution structure of an unprecedented intramolecular G-quadruplex formed by the guanosine-rich human chl1 intronic d(G(3)-N-G(4)-N(2)-G(4)-N-G(3)-N) 19-mer sequence in K(+)-containing solution...
  6. pmc OGG1 initiates age-dependent CAG trinucleotide expansion in somatic cells
    Irina V Kovtun
    Department of Pharmacology and Experimental Therapeutics, Mayo Clinic and Foundation, 200 First Street SW, Rochester, Minnesota 55905, USA
    Nature 447:447-52. 2007
    ..Age-dependent CAG expansion provides a direct molecular link between oxidative damage and toxicity in post-mitotic neurons through a DNA damage response, and error-prone repair of single-strand breaks...
  7. pmc The nuclear cap-binding complex interacts with the U4/U6·U5 tri-snRNP and promotes spliceosome assembly in mammalian cells
    Marta Pabis
    Max Planck Institute of Molecular Cell Biology and Genetics, 01307 Dresden, Germany
    RNA 19:1054-63. 2013
    The nuclear cap-binding complex (CBC) binds to the 7-methyl guanosine cap present on every RNA polymerase II transcript...
  8. pmc Bacillus subtilis RNA deprotection enzyme RppH recognizes guanosine in the second position of its substrates
    Jérémie Piton
    Centre National de la Recherche Scientifique CNRS, Unité Propre de Recherche 9073 affiliated with Université Paris Diderot, Sorbonne Paris Cité Institut de Biologie Physico Chimique, 75005 Paris, France
    Proc Natl Acad Sci U S A 110:8858-63. 2013
    ..subtilis enzyme has a binding pocket that prefers guanosine residues in the second position of its substrates...
  9. doi Electrochemical simulation of oxidation processes involving nucleic acids monitored with electrospray ionization-mass spectrometry
    Florian Pitterl
    Institute of Legal Medicine, Innsbruck Medical University, Muellerstrasse 44, 6020 Innsbruck, Austria
    Anal Bioanal Chem 397:1203-15. 2010
    ..Mass voltammograms were acquired for guanosine, adenosine, cytidine, and uridine...
  10. pmc Pumilio 2 controls translation by competing with eIF4E for 7-methyl guanosine cap recognition
    Quiping Cao
    Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
    RNA 16:221-7. 2010
    ..Thus, in addition to its suggested role in regulating poly(A) tail length and mRNA stability, our results suggest that vertebrate Pumilio can repress translation by blocking the assembly of the essential initiation complex on the cap...
  11. pmc Cap analog substrates reveal three clades of cap guanine-N2 methyltransferases with distinct methyl acceptor specificities
    Delphine Benarroch
    Molecular Biology Program, Sloan Kettering Institute, New York, New York 10065, USA
    RNA 16:211-20. 2010
    ..proteins are structurally homologous AdoMet-dependent eukaryal enzymes that methylate the N2 atom of 7-methyl guanosine nucleotides. They have an imputed role in the synthesis of the 2,2,7-trimethylguanosine (TMG) RNA cap...
  12. pmc Exon array analyses across the NCI-60 reveal potential regulation of TOP1 by transcription pausing at guanosine quartets in the first intron
    William C Reinhold
    Laboratory of Molecular Pharmacology and Developmental Therapeutics Program, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, Maryland 20894, USA
    Cancer Res 70:2191-203. 2010
    ..The observations reported here suggest the hypothesis that there is a conserved negative transcription regulator within intron 1 of the TOP1 gene associated with a quadruplex-prone region...
  13. pmc Identification of a quality-control mechanism for mRNA 5'-end capping
    Xinfu Jiao
    Department of Cell Biology and Neuroscience, Rutgers University, Piscataway, New Jersey 08854, USA
    Nature 467:608-11. 2010
    ..We propose that Rai1 is involved in an as yet uncharacterized quality control process that ensures mRNA 5'-end integrity by an aberrant-cap-mediated mRNA decay mechanism...
  14. ncbi A conformationally constrained nucleotide analogue controls the folding topology of a DNA g-quadruplex
    Pamela K Dominick
    Division of Medicinal Chemistry and Natural Products, School of Pharmacy, University of North Carolina, Chapel Hill, 27599 7360, USA
    J Am Chem Soc 126:5050-1. 2004
    ....
  15. pmc A guanosine-centric mechanism for RNA chaperone function
    Jacob K Grohman
    Department of Chemistry, University of North Carolina, Chapel Hill, NC 27599 3290, USA
    Science 340:190-5. 2013
    ..Both chaperones interacted primarily with guanosine residues...
  16. pmc Mechanisms involved in the antinociception induced by systemic administration of guanosine in mice
    A P Schmidt
    Department of Biochemistry, ICBS, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil
    Br J Pharmacol 159:1247-63. 2010
    ..p.) and oral (p.o.) administration of guanosine on mice pain models...
  17. ncbi Cloning of a novel inosine-guanosine transporter gene from Leishmania donovani by functional rescue of a transport-deficient mutant
    N S Carter
    Department of Biochemistry, Oregon Health Sciences University, Portland, Oregon 97201, USA
    J Biol Chem 275:20935-41. 2000
    ..Exploiting a mutant cell line (FBD5) of Leishmania donovani deficient in inosine and guanosine transport activity, the gene encoding this transporter (LdNT2) has been cloned by functional rescue of the ..
  18. ncbi Chlorination of guanosine and other nucleosides by hypochlorous acid and myeloperoxidase of activated human neutrophils. Catalysis by nicotine and trimethylamine
    M Masuda
    Unit of Endogenous Cancer Risk Factors, International Agency for Research on Cancer, 150 cours Albert Thomas, F 69372 Lyon Cedex 8, France
    J Biol Chem 276:40486-96. 2001
    ..react with HOCl to form chlorinated (2'-deoxy)nucleosides, including novel 8-chloro(2'-deoxy)guanosine, 5-chloro(2'-deoxy)cytidine, and 8-chloro(2'-deoxy)adenosine formed in yields of 1.6, 1.6, and 0...
  19. pmc Ribavirin is not a functional mimic of the 7-methyl guanosine mRNA cap
    Yifei Yan
    Department of Biochemistry, McIntyre Medical Sciences Building, McGill University, Montreal, Quebec H3G 1Y6, Canada
    RNA 11:1238-44. 2005
    Ribavirin is a guanosine ribonucleoside analog that displays broad-spectrum anti-viral activity and is currently used for the treatment of some viral infections...
  20. pmc Characterization of oxidative guanine damage and repair in mammalian telomeres
    Zhilong Wang
    Laboratory of Molecular Gerontology, National Institute on Aging, National Institutes of Health, Baltimore, Maryland, United States of America
    PLoS Genet 6:e1000951. 2010
    ..Our studies demonstrate that BER pathway is required in repairing oxidative guanine damage in telomeres and maintaining telomere integrity in mammals...
  21. pmc Trm11p and Trm112p are both required for the formation of 2-methylguanosine at position 10 in yeast tRNA
    Suresh K Purushothaman
    Centre de Recherche de Biochimie Macromoleculaire du CNRS, 34293 Montpellier, France
    Mol Cell Biol 25:4359-70. 2005
    N(2)-Monomethylguanosine-10 (m(2)G10) and N(2),N(2)-dimethylguanosine-26 (m(2)(2)G26) are the only two guanosine modifications that have been detected in tRNA from nearly all archaea and eukaryotes but not in bacteria...
  22. pmc Biochemical and genetic analysis of RNA cap guanine-N2 methyltransferases from Giardia lamblia and Schizosaccharomyces pombe
    Stephane Hausmann
    Molecular Biology Program, Sloan Kettering Institute, New York, NY 10021, USA
    Nucleic Acids Res 35:1411-20. 2007
    ..Deletion of the pcm1(+) gene was lethal, as were missense mutations in the Pcm1 active site. Thus, whereas m(7)G caps are essential in both S. pombe and S. cerevisiae, m(2,2,7)G caps are not...
  23. pmc Accumulation of premutagenic DNA lesions in mice defective in removal of oxidative base damage
    A Klungland
    Imperial Cancer Research Fund, Clare Hall Laboratories, South Mimms, Hertfordshire EN6 3LD, United Kingdom
    Proc Natl Acad Sci U S A 96:13300-5. 1999
    ....
  24. doi Inhibition of dicing of guanosine-rich shRNAs by quadruplex-binding compounds
    Anja Henn
    Department of Chemistry and Konstanz Research School of Chemical Biology KoRS CB, University of Konstanz, Universitatsstrasse 10, 78457 Konstanz, Germany
    Chembiochem 9:2722-9. 2008
    ..Here, we present a first step in this direction by augmenting shRNAs with guanosine-rich sequences that are prone to fold into four-stranded structures...
  25. doi The thermodynamics of 3'-terminal pyrene and guanosine for the design of isoenergetic 2'-O-methyl-RNA-LNA chimeric oligonucleotide probes of RNA structure
    Anna Pasternak
    Institute of Bioorganic Chemistry, Polish Academy of Sciences, 60 714 Poznan, Noskowskiego 12 14, Poland
    Biochemistry 47:1249-58. 2008
    ..probes for RNA, we report the influence of adding 5'- or 3'-terminal 2'-O-methylguanosine (GM), LNA-guanosine (GL), or 3'-terminal pyrene pseudo-nucleotide (PPN) on the thermodynamic stability of 2'-O-methyl-RNA/RNA (2'-O-..
  26. ncbi Guanosine and inosine display antioxidant activity, protect DNA in vitro from oxidative damage induced by reactive oxygen species, and serve as radioprotectors in mice
    Sergey V Gudkov
    Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Moscow Region, 142290 Russian Federation
    Radiat Res 165:538-45. 2006
    ..b>Guanosine and inosine considerably reduced deamination of cytosine in the DNA solutions upon heating for 24 h at 80 ..
  27. pmc Structure of radicals from X-irradiated guanine derivatives: an experimental and computational study of sodium guanosine dihydrate single crystals
    Nayana Jayatilaka
    Department of Physics and Astronomy, Georgia State University, P O Box 4106, Atlanta, Georgia 30302 4106, USA
    J Phys Chem B 111:800-10. 2007
    In sodium guanosine dihydrate single crystals, the guanine moiety is deprotonated at N1 due to growth from high-pH (>12) solutions...
  28. ncbi Adenosine, inosine, and guanosine protect glial cells during glucose deprivation and mitochondrial inhibition: correlation between protection and ATP preservation
    M S Jurkowitz
    Department of Medical Biochemistry, Ohio State University, Columbus 43210, USA
    J Neurochem 71:535-48. 1998
    The purpose of this study was to determine the mechanism by which adenosine, inosine, and guanosine delay cell death in glial cells (ROC-1) that are subjected to glucose deprivation and mitochondrial respiratory chain inhibition with ..
  29. doi Guanosine and synthetic organoselenium compounds modulate methylmercury-induced oxidative stress in rat brain cortical slices: involvement of oxidative stress and glutamatergic system
    Daniel H Roos
    Departamento de Quimica, Centro de Ciencias Naturais e Exatas, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil
    Toxicol In Vitro 23:302-7. 2009
    ..of glutamatergic system in methylmercury (MeHg) neurotoxicity and whether diphenyl diselenide, ebselen and guanosine could protect cortical rat brain slices from MeHg-induced ROS generation...
  30. doi Influence of water hydrogen bonding on the reactions of arylnitrenium ions with guanosine: hydrogen-bonding effects can favor reaction at the C8 site
    Zhen Guo
    Department of Chemistry, The University of Hong Kong, Pokfulam Road, Hong Kong Special Administrative Region, People s Republic of China
    J Phys Chem B 113:6528-32. 2009
    A computational study was done for the reactions of the 2-fluorenylnitrenium ion (2FN) with guanosine (G) and its monohydrate tautomer (G...
  31. doi Protective effects of guanosine against sepsis-induced damage in rat brain and cognitive impairment
    Fabricia Petronilho
    Laboratório de Fisiopatologia Experimental e Instituto Nacional de Ciência e Tecnologia Translacional em Medicina, Programa de Pos Graduacao em Ciencias da Saude, Universidade do Extremo Sul Catarinense, Criciuma, SC, Brazil
    Brain Behav Immun 26:904-10. 2012
    ..We have assessed the effects of acute and extended administration of guanosine (GUA) on brain oxidative stress parameters and cognitive impairment in rats submitted to sepsis by cecal ..
  32. ncbi The cytotoxic and anti-proliferative effects of 3-hydrogenkwadaphnin in K562 and jurkat cells is reduced by guanosine
    Mohammad Amin Moosavi
    Institute of Biochemistry and Biophysics, P O Box 13145 1384, University of Tehran, Tehran, Iran
    J Biochem Mol Biol 38:391-8. 2005
    ..Exogenous addition of 25-50 microM guanosine and/or deoxyguanosine to the cell culture of the drug-treated cells restored DNA synthesis, released cell arrest ..
  33. doi Guanosine controls inflammatory pathways to afford neuroprotection of hippocampal slices under oxygen and glucose deprivation conditions
    Tharine Dal-Cim
    Departamento de Bioquimica, Centro de Ciencias Biologicas, Universidade Federal de Santa Catarina, Florianopolis, SC, Brazil
    J Neurochem 126:437-50. 2013
    b>Guanosine (GUO) is an endogenous modulator of glutamatergic excitotoxicity and has been shown to promote neuroprotection in in vivo and in vitro models of neurotoxicity...
  34. doi Synthesis of guanosine 5'-conjugates and their use as initiator molecules for transcription priming
    Jörn Wolf
    Ernst Moritz Arndt Universitat Greifswald, Institut fur Biochemie, Bioorganische Chemie, Felix Hausdorff Strasse 4, 17487, Greifswald, Germany
    Org Biomol Chem 6:899-907. 2008
    We have synthesised two guanosine derivatives that are linked to biotinylated adenosine moieties by using two different strategies, one that includes synthetic steps on the solid phase and another one that is performed entirely in ..
  35. ncbi UVA irradiation induces relocalisation of the DNA repair protein hOGG1 to nuclear speckles
    Anna Campalans
    Departement de Radiobiologie et Radiopathologie, Commissariat a l Energie Atomique, UMR 217 CNRS CEA, 18 route du Panorama, 92265 Fontenay aux Roses, France
    J Cell Sci 120:23-32. 2007
    ..The recruitment of DNA repair proteins to nuclear speckles after oxidative stress implicates these organelles in the cellular stress response...
  36. doi Extracellular guanosine and GTP promote expression of differentiation markers and induce S-phase cell-cycle arrest in human SH-SY5Y neuroblastoma cells
    S Guarnieri
    Dipartamento Scienze Mediche di Base ed Applicate Università G d Annunzio, Chieti, Italy
    Int J Dev Neurosci 27:135-47. 2009
    ..Using this system, we asked whether guanosine (Guo) or guanosine-5'-triphosphate (GTP) are able to drive differentiation towards one particular phenotype...
  37. ncbi Regulation of alternative splicing by RNA editing
    S M Rueter
    Department of Pharmacology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 6600, USA
    Nature 399:75-80. 1999
    ..analysis of ADAR2 genomic DNA revealed the presence of adenosine-adenosine (AA) and adenosine-guanosine (AG) dinucleotides at these proximal and distal alternative 3' acceptor sites, respectively...
  38. pmc Monitoring translation with modified mRNAs strategically labeled with isomorphic fluorescent guanosine mimetics
    Wei Liu
    Department of Chemistry, University of Pennsylvania, 231 S 34 Street, Philadelphia, Pennsylvania 19104, United States
    ACS Chem Biol 8:2017-23. 2013
    ..mRNAs, site-specifically modified at codon positions 4, 5, and 6 with a new emissive and responsive isosteric guanosine mimetic ((th)G), with the goal of developing real time assays for monitoring translation-related events at ..
  39. ncbi Quantitation of urinary nucleosides by high-performance liquid chromatography
    H M Liebich
    , , Germany
    J Chromatogr A 763:193-7. 1997
    ..37), pseudouridine (25.52), cytidine (0.07), uridine (0.21), 1-methyladenosine (2.19), inosine (0.30), guanosine (0.06), xanthosine (0.59), 3-methyluridine (0.11), 1-methylinosine (1.13). 1-methylguanosine (0...
  40. ncbi Electron transfer between guanosine radical and amino acids in aqueous solution. 1. Reduction of guanosine radical by tyrosine
    Olga B Morozova
    International Tomography Center of SB RAS, Novosibirsk, Russia
    J Phys Chem B 111:7439-48. 2007
    ..repair, the reductive electron transfer from the aromatic amino acid tyrosine to the radical of the purine base guanosine monophosphate (GMP) was studied by time-resolved chemically induced dynamic nuclear polarization (CIDNP)...
  41. ncbi Guanosine protects human neuroblastoma cells from oxidative stress and toxicity induced by Amyloid-beta peptide oligomers
    A Tarozzi
    Department of Pharmacology, Alma Mater Studiorum University of Bologna, Bologna, Italy
    J Biol Regul Homeost Agents 24:297-306. 2010
    ..We therefore investigated the effect of guanosine (GUO), a guanine based purine, that exerts neurotrophic and neuroprotective effects...
  42. doi Extracellular cyclic GMP and its derivatives GMP and guanosine protect from oxidative glutamate toxicity
    Philipp Albrecht
    Department of Neurology, Heinrich Heine Universitat Dusseldorf, Dusseldorf, Germany
    Neurochem Int 62:610-9. 2013
    ..extracellular peptide or small molecule ligands for effects on cell death and identified extracellular cyclic guanosine monophosphate (cGMP) as a protective substance...
  43. doi Electrocatalytic oxidation behavior of guanosine at graphene, chitosan and Fe3O4 nanoparticles modified glassy carbon electrode and its determination
    Huanshun Yin
    College of Chemistry and Material Science, Shandong Agricultural University, Taian 271018, Shandong, China
    Talanta 82:1193-9. 2010
    ..The electrochemical oxidation behavior of guanosine was investigated in pH 7.0 phosphate buffer solution by cyclic voltammetry and differential pulse voltammetry...
  44. ncbi Role of guanosine kinase in the utilization of guanosine for nucleotide synthesis in Escherichia coli
    B Hove-Jensen
    Enzyme Division, University Institute of Biological Chemistry B, Copenhagen K, Denmark
    J Gen Microbiol 135:1263-73. 1989
    ..in the pathways of interconversion and salvage of purine compounds, we demonstrated the in vivo function of guanosine kinase and inosine kinase...
  45. ncbi Interaction of human nuclear topoisomerase I with guanosine quartet-forming and guanosine-rich single-stranded DNA and RNA oligonucleotides
    Christophe Marchand
    Laboratory of Molecular Pharmacology, Center for Cancer Research, NCI, National Institutes of Health, Bethesda, Maryland 20892 4255, USA
    J Biol Chem 277:8906-11. 2002
    ..In this study, we show that intra- and intermolecular guanosine quartets (G-quartets) can inhibit top1-mediated DNA cleavage at a high affinity site...
  46. pmc Guanosine supplementation reduces apoptosis and protects renal function in the setting of ischemic injury
    K J Kelly
    Department of Medicine, Division of Nephrology, Indiana University, Indianapolis, Indiana 46202, USA
    J Clin Invest 108:1291-8. 2001
    ..renal artery clamp for 32 minutes to induce ischemia and then received either normal saline ("NS") or guanosine ("G")...
  47. doi Identifying guanosine self assembly at natural isotopic abundance by high-resolution 1H and 13C solid-state NMR spectroscopy
    Amy L Webber
    Department of Physics, University of Warwick, Coventry CV4 7AL, United Kingdom
    J Am Chem Soc 133:19777-95. 2011
    ..CRAMPS) solid-state NMR correlation spectra, ribbon-like and quartet-like self-assembly can be identified for guanosine derivatives without isotopic labeling for which it was not possible to obtain single crystals suitable for ..
  48. pmc Quantitation of four guanine oxidation products from reaction of DNA with varying doses of peroxynitrite
    Hongbin Yu
    Biological Engineering Division, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Room 56 731A, Cambridge, Massachusetts 02139, USA
    Chem Res Toxicol 18:1849-57. 2005
    ..The complexity of these dose-response relationships is likely due to the dual role of peroxynitrite as both an oxidant and a nucleophile in competition with water...
  49. doi Neuroprotective effects of guanosine on stroke models in vitro and in vivo
    Ruby Chang
    Department of Neurosurgery, McMaster University, Health Sciences Centre, 4N71B, 1200 Main Street West, Hamilton, ON L8N 3Z5, Canada
    Neurosci Lett 431:101-5. 2008
    ..After combined glucose and oxygen deprivation (CGOD) addition of guanosine (100 microM), a non-adenine-based purine nucleoside, significantly reduced the proportion of cells undergoing ..
  50. pmc The purine nucleosides adenosine and guanosine delay axonal degeneration in vitro
    Craig Press
    Department of Pathology, Hope Center for Neurological Disorders, Washington University School of Medicine, St Louis, Missouri, USA
    J Neurochem 109:595-602. 2009
    ..In this study, we show that the purine nucleosides adenosine and guanosine, but not inosine, inhibit injury-induced axonal degeneration in cultured dorsal root ganglia neurons...
  51. ncbi Nucleotide mutations in purA gene and pur operon promoter discovered in guanosine- and inosine-producing Bacillus subtilis strains
    Jiangchao Qian
    State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, PR China
    Biotechnol Lett 28:937-41. 2006
    ..55 (relative to translation start site) in purA gene was found in a high inosine-producing strain and in a high guanosine-producing strain, which correlates with the absence of adenylosuccinate synthetase activity in these strains...
  52. ncbi In vivo quinolinic acid increases synaptosomal glutamate release in rats: reversal by guanosine
    Rejane G Tavares
    Department of Biochemistry, ICBS, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil
    Neurochem Res 30:439-44. 2005
    ..Guanine-based purines (GBPs) (guanosine and GMP) have been shown to exert neuroprotective effects against glutamatergic excitotoxic events...
  53. ncbi Comparison of the reactivity of oxaliplatin, pt(diaminocyclohexane)Cl2 and pt(diaminocyclohexane1)(OH2)2(2+) with guanosine and L-methionine
    S Verstraete
    Laboratoire de Pharmacologie et de Toxicologie, Centre Hospitalier Universitaire, Angers, France
    J Inorg Biochem 84:129-35. 2001
    The initial rates of reactivity of oxaliplatin, its metabolites Pt(dach)Cl2 and Pt(dach)(OH2)2(2+) with guanosine and L-met in water, NaCl and phosphate were compared...
  54. ncbi Ontogenetic profile of glutamate uptake in brain structures slices from rats: sensitivity to guanosine
    Ana Paula Thomazi
    Departamento de Bioquimica, Instituto de Ciencias Basicas da Saude, Universidade Federal do Rio Grande do Sul, UFRGS, Ramiro Barcelos, 2600 Anexo, CEP 90035 003, Porto Alegre, RS, Brazil
    Mech Ageing Dev 125:475-81. 2004
    ..brain to describe the in vitro profile of glutamate uptake during development and ageing, and its sensitivity to guanosine. In all structures, glutamate uptake was higher in immature animals...
  55. ncbi A molecular code for splicing silencing: configurations of guanosine-rich motifs
    P J Grabowski
    Department of Biological Sciences, University of Pittsburgh HHMI, Pittsburgh, PA 15260, USA
    Biochem Soc Trans 32:924-7. 2004
    ..We identify a set of guanosine-rich motifs that function co-operatively to regulate the CI cassette exon in a manner consistent with its in ..
  56. pmc Synthesis of guanosine and deoxyguanosine phosphoramidites with cross-linkable thioalkyl tethers for direct incorporation into RNA and DNA
    Xiaorong Hou
    Department of Chemistry and Chemical Biology, Rutgers, The State University of New Jersey, Piscataway, New Jersey 08854, USA
    Nucleosides Nucleotides Nucleic Acids 28:1076-94. 2009
    We describe the synthesis of protected phosphoramidites of deoxyriboguanosine and guanosine derivatives containing a thiopropyl tether at the guanine N2 (7a,b) for site-specific crosslinking from the minor groove of either DNA or RNA to ..
  57. ncbi Guanosine inhibits CD40 receptor expression and function induced by cytokines and beta amyloid in mouse microglia cells
    Iolanda D'Alimonte
    Department of Biomedical Sciences, Section of Pharmacology, University of Chieti, Via dei Vestini 29, 66013 Chieti, Italy
    J Immunol 178:720-31. 2007
    ..To this aim, we investigated the effect of guanosine, a purine nucleoside that exerts neurotrophic and neuroprotective effects...
  58. ncbi Characterization of guanine and guanosine transport in primary cultured rat cortical astrocytes and neurons
    Kazuki Nagasawa
    Department of Environmental Biochemistry, Kyoto Pharmaceutical University, Yamashina ku, Kyoto, Japan
    Glia 55:1397-404. 2007
    In this study, we examined the transport mechanisms for guanine and guanosine in rat neurons and astrocytes, and compared their characteristics...
  59. doi Guanosine hydrogen-bonded scaffolds: a new way to control the bottom-up realisation of well-defined nanoarchitectures
    Stefano Lena
    Dipartimento di Chimica Organica A Mangini, Alma Mater Studiorum Universita di Bologna, Via San Giacomo 11, 40126 Bologna, Italy
    Chemistry 15:7792-806. 2009
    Over the last two decades, guanosine-related molecules have been of interest in different areas, ranging from structural biology to medicinal chemistry, supramolecular chemistry and nanotechnology...
  60. pmc Three modifications in the D and T arms of tRNA influence translation in Escherichia coli and expression of virulence genes in Shigella flexneri
    Jaunius Urbonavicius
    Department of Molecular Biology, Umea University, S 90 187 Umeå, Sweden
    J Bacteriol 184:5348-57. 2002
    ..Moreover, lack of Psi55 in tRNA of human pathogen Shigella flexneri leads to a reduced expression of several virulence-associated genes...
  61. ncbi Glutamate uptake in cultured astrocytes depends on age: a study about the effect of guanosine and the sensitivity to oxidative stress induced by H(2)O(2)
    Carmem Gottfried
    Departamento de Ciencias Fisiologicas, Faculdade de Biociencias, Pontifi cia Universidade Católica do Rio Grande do Sul, PUCRS, RS, Porto Alegre, Brazil
    Mech Ageing Dev 123:1333-40. 2002
    ..Addition of guanosine stimulated glutamate uptake only in 10 DIV astrocytes...
  62. ncbi Spiroiminodihydantoin is the major product of the 8-oxo-7,8-dihydroguanosine reaction with peroxynitrite in the presence of thiols and guanosine photooxidation by methylene blue
    J C Niles
    Division of Bioengineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Room 56-731A, Cambridge, Massachusetts 02139, USA
    Org Lett 3:963-6. 2001
    ..Additionally, we have found that the spiroiminodihydantoin, and not the previously reported 4-hydroxy-8-oxo-4,8-dihydroguanosine, is the major final product formed during the methylene blue-mediated photooxidation of guanosine.
  63. ncbi Guanosine promotes the up-regulation of inward rectifier potassium current mediated by Kir4.1 in cultured rat cortical astrocytes
    Valentina Benfenati
    Department of Human and General Physiology, University of Bologna, Bologna, Italy
    J Neurochem 98:430-45. 2006
    b>Guanosine (Guo) is an endogenous neuroprotective molecule of the CNS, which has various acute and long-term effects on both neurones and astroglial cells...
  64. ncbi Neurite outgrowth in PC12 cells is enhanced by guanosine through both cAMP-dependent and -independent mechanisms
    J W Gysbers
    Department of Biomedical Sciences, Health Sciences Centre, McMaster University, Hamilton, Ontario, Canada
    Neurosci Lett 220:175-8. 1996
    Extracellular guanosine, guanosine triphosphate (GTP), and 5'-N'-ethylcarboxamidoadenosine (NECA), each significantly enhanced the proportion of nerve growth factor (NGF)-treated rat pheochromocytoma (PC12) cells which had neurites, ..
  65. ncbi gsk disruption leads to guanosine accumulation in Escherichia coli
    H Matsui
    Fermentation and Biotechnology Laboratories, Ajinomoto Co, Inc, Kawasaki shi, Kanagawa, Japan
    Biosci Biotechnol Biochem 65:1230-5. 2001
    ..Alternatively, the gsk (guanosine-inosine kinase gene) disruption caused a considerable amount of guanosine accumulation together with a slight ..
  66. doi Intradermal cytosine-phosphate-guanosine treatment reduces lung inflammation but induces IFN-γ-mediated airway hyperreactivity in a murine model of natural rubber latex allergy
    Rita Haapakoski
    Unit of Excellence for Immunotoxicology, Finnish Institute of Occupational Health, Helsinki, Finland
    Am J Respir Cell Mol Biol 44:639-47. 2011
    ..Immunostimulatory cytosine-phosphate-guanosine (CpG)-oligodeoxynucleotide motifs are candidate molecules for immunotherapeutic studies, as they have been shown ..
  67. doi Neuroprotective effect of guanosine against glutamate-induced cell death in rat hippocampal slices is mediated by the phosphatidylinositol-3 kinase/Akt/ glycogen synthase kinase 3β pathway activation and inducible nitric oxide synthase inhibition
    Simone Molz
    Departamento de Bioquimica, Centro de Ciencias Biologicas, Universidade Federal de Santa Catarina, Trindade, Florianopolis, SC, Brasil molz s hotmail com
    J Neurosci Res 89:1400-8. 2011
    ..b>Guanosine, an endogenous guanine nucleoside, prevents excitotoxicity by its ability to modulate glutamate transport...
  68. doi The encapsulation and release of guanosine from PEGylated liposomes
    Yan Er
    Ian Wark Research Institute, University of South Australia, Mawson Lakes, SA, Australia
    J Liposome Res 19:29-36. 2009
    The encapsulation and release kinetics of guanosine from liposomes and polyethylene glycol (PEG)-modified liposomes are reported...
  69. ncbi Purine nucleoside phosphorylase. Structure-activity relationships for substrate and inhibitor properties of N-1-, N-7-, and C-8-substituted analogues; differentiation of mammalian and bacterial enzymes with N-1-methylinosine and guanosine
    A Bzowska
    Department of Biophysics, University of Warsaw, Poland
    J Biol Chem 263:9212-7. 1988
    ..Included also are guanosine analogues with C-8 substituents (alpha-hydroxyisopropyl, methyl, bromo, chloro, amino) with known populations of ..
  70. doi Systemic administration of guanosine promotes functional and histological improvement following an ischemic stroke in rats
    Michel P Rathbone
    Department of Medicine Neurology, Neurobiochemistry, Health Sciences Centre, Room 4E15, 1200 Main Street West, Hamilton, ON, Canada L8N 3Z5
    Brain Res 1407:79-89. 2011
    Previously we have found that extracellular guanosine (Guo) has neuroprotective properties in in vitro and in vivo...
  71. ncbi Rat brain guanosine binding site. Biological studies and pseudo-receptor construction
    Ugo Traversa
    Dipartimento di Scienze Biomediche BRAIN Center, via L Giorgieri 7, Universita di Trieste, 34127 Trieste, Italy
    Bioorg Med Chem 11:5417-25. 2003
    Rat brain guanosine binding sites were studied by (i)...
  72. ncbi Maturation of pre-tRNA(fMet) by Escherichia coli RNase P is specified by a guanosine of the 5'-flanking sequence
    T Meinnel
    Laboratoire de Biochimie, CNRS, Ecole Polytechnique, Palaiseau, France
    J Biol Chem 270:15908-14. 1995
    ..Notably, this G is the only residue that is conserved in the 5'-flanking sequences of all four E. coli tRNA(fMet) genes...
  73. ncbi Spiroiminodihydantoin and guanidinohydantoin are the dominant products of 8-oxoguanosine oxidation at low fluxes of peroxynitrite: mechanistic studies with 18O
    Jacquin C Niles
    Biological Engineering Division and Department of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Room 56 738A, Cambridge, Massachusetts 02139, USA
    Chem Res Toxicol 17:1510-9. 2004
    ..To explain the pH-dependent Gh and Sp yields, we propose that 5 has a pKa approximately 5.8 and that the differential reactivity of the protonated and deprotonated form of 5 leads to its partitioning into Gh and Sp, respectively...
  74. ncbi The intron 1 of HPV 16 has a suboptimal branch point at a guanosine
    Marco Antonio De la Rosa-Rios
    Depto Biomedicina Molecular, Centro de Investigacion y de Estudios Avanzados, Mexico
    Virus Res 118:46-54. 2006
    ..bound at the 5' end of the intron are probably the authentic branch point sequence, and all of them branch at guanosine 328 instead of the typical adenosine...
  75. pmc Mycophenolate mofetil inhibits human Tenon fibroblast proliferation by guanosine depletion
    C Heinz
    Department of Ophthalmology, University of Essen, Hufelandstrasse 55, 45122 Essen, Germany
    Br J Ophthalmol 87:1397-8. 2003
    ..In this study the authors set out to investigate whether growth inhibition of mycophenolate mofetil on human Tenon fibroblasts is mediated by guanosine depletion.
  76. ncbi The purine nucleoside cycle in cell-free extracts of rat brain: evidence for the occurrence of an inosine and a guanosine cycle with distinct metabolic roles
    C Barsotti
    Department of Physiology and Biochemistry, University of Pisa, Via Santa Maria 55, 56126 Pisa, Italy
    Cell Mol Life Sci 60:786-93. 2003
    ..When the cycle is started by addition of Pi and guanine (the 'guanosine cycle'), xanthine and xanthosine are formed, in addition to GMP and guanosine, showing that the guanosine cycle '..
  77. pmc Complete genome sequence of Bacillus amyloliquefaciens TA208, a strain for industrial production of guanosine and ribavirin
    Guoqiang Zhang
    Department of Industrial Microbiology and Biotechnology, Institute of Microbiology, Chinese Academy of Sciences, Chaoyang District, Beijing 100101, China
    J Bacteriol 193:3142-3. 2011
    ..report the complete genome sequence of Bacillus amyloliquefaciens TA208, a strain for industrial production of guanosine and synthesis of ribavirin by assimilation of formamide...
  78. pmc Genetic evidence for the essential role of PfNT1 in the transport and utilization of xanthine, guanine, guanosine and adenine by Plasmodium falciparum
    Kamal El Bissati
    Department of Genetics and Developmental Biology, University of Connecticut Health Center, Farmington, CT 06030, USA
    Mol Biochem Parasitol 161:130-9. 2008
    ..knockout parasites to transport and utilize the other physiologically relevant purines adenine, guanine, guanosine and xanthine was also examined. Unlike wild-type and complemented P...
  79. ncbi Guanosine-induced decrease in side population of lung cancer cells: lack of correlation with ABCG2 expression
    C Su
    Department of Surgery Neurosurgery, Neurobiology, Hamilton Neurorestorative Group, McMaster University, Health Sciences Centre, Hamilton, Canada
    J Biol Regul Homeost Agents 24:19-25. 2010
    ..b>Guanosine is an important intercellular signalling molecule; it stimulates stem cell proliferation in vivo and affects ..
  80. ncbi Anticonvulsant effect of GMP depends on its conversion to guanosine
    Félix A Soares
    Departamento de Bioquimica, Instituto de Ciencias Basicas da Saude, Universidade Federal do Rio Grande do Sul, Rua Ramiro Barcelos 2600 Anexo CEP 90 035 003, Porto Alegre, RS, Brazil
    Brain Res 1005:182-6. 2004
    ..In vivo, both guanosine 5'-monophosphate (GMP) and guanosine (GUO) protected against seizures...
  81. ncbi Intriguing mass spectrometric behavior of guanosine under low energy collision-induced dissociation: H2O adduct formation and gas-phase reactions in the collision cell
    Robin Tuytten
    Department of Chemistry, Nucleoside Research Unit corrected University of Antwerp, Antwerp, Belgium
    J Am Soc Mass Spectrom 16:1291-304. 2005
    An in-depth study of the fragmentation pathway of guanosine was conducted by using an in-source collision-induced dissociation high-mass accuracy tandem mass spectrometry experiment...
  82. ncbi 7-Functionalized 7-deazapurine ribonucleosides related to 2-aminoadenosine, guanosine, and xanthosine: glycosylation of pyrrolo[2,3-d]pyrimidines with 1-O-acetyl-2,3,5-tri-O-benzoyl-D-ribofuranose
    Frank Seela
    Laboratorium fur Organische und Bioorganische Chemie, Institut fur Chemie, Universitat Osnabruck, Barbarastr 7, D 49069 Osnabruck, Germany
    J Org Chem 71:81-90. 2006
    ..transformed to a number of novel 7-halogenated 7-deazapurine ribonucleosides (2b-d, 3b-d, and 4b-d) related to guanosine, 2-aminoadenosine, and xanthosine...
  83. ncbi Guanosine and GMP prevent seizures induced by quinolinic acid in mice
    A P Schmidt
    Department of Biochemistry, ICBS, Federal University of Rio Grande do Sul, Avenida Ramiro Barcelos, 2600 Anexo, CEP 90035 003, Porto Alegre, Brazil
    Brain Res 864:40-3. 2000
    ..Guanine-based purines, including the nucleoside guanosine and the nucleotide GMP, have been shown to antagonize glutamatergic activity at the receptor level and the other ..
  84. pmc Guanosine nucleotides inhibit different syndromes of PTHrP excess caused by human cancers in vivo
    Wolfgang E Gallwitz
    OsteoScreen Ltd, San Antonio, Texas 78229, USA
    J Clin Invest 110:1559-72. 2002
    ..These results suggest that the PTHrP gene promoter may be a suitable target for treating the skeletal effects of malignancy...
  85. doi Importance of platinum(II)-assisted platinum(IV) substitution for the oxidation of guanosine derivatives by platinum(IV) complexes
    Sunhee Choi
    Department of Chemistry and Biochemistry, Middlebury College, Middlebury, VT 05753, USA
    Inorg Chem 47:1352-60. 2008
    b>Guanosine derivatives with a nucleophilic group at the 5' position (G-5') are oxidized by the Pt (IV) complex Pt( d, l)(1,2-(NH 2) 2C 6H 10)Cl 4 ([Pt (IV)(dach)Cl 4])...
  86. ncbi Use of 13C as an indirect tag in 15N specifically labeled nucleosides. Syntheses of [8-13C-1,7,NH2-15N3]adenosine, -guanosine, and their deoxy analogues
    Anthony J Shallop
    Department of Chemistry and Chemical Biology, Rutgers, The State University of New Jersey, Piscataway, New Jersey 08854, USA
    J Org Chem 68:8657-61. 2003
    ..Here, we present methods for the syntheses of [8-13C-1,7,NH2-15N3]adenosine, -guanosine, and their deoxy analogues.
  87. pmc Endothelium-dependent and -independent effects of exogenous ATP, adenosine, GTP and guanosine on vascular tone and cyclic nucleotide accumulation of rat mesenteric artery
    P Vuorinen
    Department of Biomedical Sciences, University of Tampere, Finland
    Br J Pharmacol 105:279-84. 1992
    1. The effects of exogenous guanosine 5'-triphosphate (GTP) and guanosine on vascular tone and cyclic nucleotide accumulation of noradrenaline-precontracted endothelium-intact and endothelium-denuded rat mesenteric artery rings were ..
  88. ncbi Extracellular conversion of guanine-based purines to guanosine specifically enhances astrocyte glutamate uptake
    Marcos Emilio dos Santos Frizzo
    Departamento de Bioquimica, Universidade Federal do Rio Grande do Sul, Rua Ramiro Barcelos, 2600 Anexo, 90035 003 Porto Alegre, RS, Brazil
    Brain Res 972:84-9. 2003
    b>Guanosine (GUO) has been shown to stimulate glutamate uptake in primary astrocyte cultures...
  89. ncbi Effect of graded corticosterone treatment on aging-related markers of oxidative stress in rat liver mitochondria
    Pilar Caro
    Department of Animal Physiology II, Facultad de Ciencias Biologicas, Complutense University, c Antonio Novais 2, Madrid 28040, Spain
    Biogerontology 8:1-11. 2007
    ..However, they show that this hormone modulates the level of oxidative stress both in proteins and in mtDNA. Some of these changes can contribute to the chronic effects of the hormone at tissue level...
  90. ncbi Guanosine distribution and oxidation resistance in eight eukaryotic genomes
    Keith A Friedman
    Department of Chemical Engineering and the Texas Materials Institute, University of Texas at Austin, Austin, Texas 78712 0231, USA
    J Am Chem Soc 126:2368-71. 2004
    Reactive oxygen species that attack DNA are continuously generated in living cells. Both the guanosine (G) mole fraction and its distribution should affect the stability of genomes and their parts to oxidation...
  91. ncbi Direct observation of a cytosine analogue that forms five hydrogen bonds to guanosine: guanidino G-clamp
    Christopher J Wilds
    Department of Biological Sciences, Vanderbilt University Nashville, TN 37235, USA
    Angew Chem Int Ed Engl 41:115-7. 2002
  92. doi Guanosine + OH radical reaction in aqueous solution: a reinterpretation of the UV-vis data based on thermodynamic and kinetic calculations
    Annia Galano
    Departamento de Quimica, Universidad Autonoma Metropolitana Iztapalapa, 09340 Mexico D F, Mexico
    Org Lett 11:5114-7. 2009
    ..and kinetic calculations have been used to reinterpret the UV-vis data related to the OH radical oxidation of guanosine. The main channel of reaction (70-75%) is proposed to be the formation of a guanosine radical cation followed by ..
  93. ncbi Calcium-stimulated guanosine--inosine nucleosidase from yellow lupin (Lupinus luteus)
    Maciej Szuwart
    Department of Biochemistry and Biotechnology, Agricultural University, 35 Wołyńska Street, 60 637 Poznan, Poland
    Phytochemistry 67:1476-85. 2006
    b>Guanosine-inosine-preferring nucleoside N-ribohydrolase has been purified to homogeneity from yellow lupin (Lupinus luteus) seeds by ammonium sulfate fractionation, ion-exchange chromatography and gel filtration...
  94. pmc Genetic and biochemical analysis of yeast and human cap trimethylguanosine synthase: functional overlap of 2,2,7-trimethylguanosine caps, small nuclear ribonucleoprotein components, pre-mRNA splicing factors, and RNA decay pathways
    Stephane Hausmann
    Department of Microbiology and Molecular Medicine, University of Geneva, CH1211 Geneva, Switzerland
    J Biol Chem 283:31706-18. 2008
    ..We present a biochemical characterization of the human Tgs1 guanine-N2 methyltransferase reaction and identify individual amino acids required for methyltransferase activity in vitro and in vivo...
  95. doi Automated method for the direct analysis of 8-oxo-guanosine and 8-oxo-2'-deoxyguanosine in human urine using ultraperformance liquid chromatography and tandem mass spectrometry
    Trine Henriksen
    Laboratory of Clinical Pharmacology Q7642, Rigshospitalet, Blegdamsvej 9, DK 2100 Copenhagen, Denmark
    Free Radic Biol Med 47:629-35. 2009
    ..Accuracy and precision were documented, showing average recoveries of 106.2% (8-oxoGuo) and 106.9% (8-oxodG), and overall precision (within-day and between-days) of 6.1 and 4.4%, respectively...
  96. ncbi Synthesis, physicochemical and biochemical studies of 1',2'-oxetane constrained adenosine and guanosine modified oligonucleotides, and their comparison with those of the corresponding cytidine and thymidine analogues
    Pushpangadan I Pradeepkumar
    Contribution from the Department of Bioorganic Chemistry, Box 581, Biomedical Center, University of Uppsala, S 75123 Uppsala, Sweden
    J Am Chem Soc 126:11484-99. 2004
    ....
  97. ncbi Efficiency of repair of an abasic site within DNA clustered damage sites by mammalian cell nuclear extracts
    Martine E Lomax
    Radiation and Genome Stability Unit, Medical Research Council, Harwell, Didcot, Oxfordshire OX11 0RD, UK
    Biochemistry 43:11017-26. 2004
    ..This study provides new insight into understanding the processes that lead to the biological consequences of radiation-induced DNA damage and ultimately tumorigenesis...
  98. pmc Structure and potential mutagenicity of new hydantoin products from guanosine and 8-oxo-7,8-dihydroguanine oxidation by transition metals
    Cynthia J Burrows
    Department of Chemistry, University of Utah, 315 S 1400 East, Salt Lake City, UT 84112 0850, USA
    Environ Health Perspect 110:713-7. 2002
    ..These products are also formed by the overall four-electron oxidation of guanosine by photochemical processes involving O(2)...
  99. ncbi Supramolecular architectures generated by self-assembly of guanosine derivatives
    Jeffery T Davis
    Department of Chemistry and Biochemistry, University of Maryland, College Park, Maryland 20742, USA
    Chem Soc Rev 36:296-313. 2007
    ..begins with a brief historical context of G self-assembly in water and then describes studies on lipophilic guanosine analogs in organic solvents...
  100. ncbi Giardia lamblia RNA cap guanine-N2 methyltransferase (Tgs2)
    Stephane Hausmann
    Molecular Biology Program, Sloan Kettering Institute, New York, New York 10021, USA
    J Biol Chem 280:32101-6. 2005
    ..The specificity of Tgs2 raises the prospect that some Giardia mRNAs might contain dimethylguanosine caps...
  101. ncbi P2Y2 receptor up-regulation induced by guanosine or UTP in rat brain cultured astrocytes
    P Ballerini
    Dept of Biomedical Sciences, Section of Pharmacology and Toxicology, Medical School, University G d Annunzio of Chieti Pescara, Via dei Vestini 29, 66013 Chieti, Italy
    Int J Immunopathol Pharmacol 19:293-308. 2006
    ..We showed that not only UTP and ATP are the most potent and naturally occurring agonist for P2Y2 sites, but also guanosine induced an up-regulation of astrocyte P2Y2 receptor mRNA evaluated by Northern blot analysis...

Research Grants71

  1. DNA methylation of extracellular superoxide dismutase in pulmonary hypertension
    EVA NOZIK GRAYCK; Fiscal Year: 2012
    ..modifications including cytosine methylation within the promoter region, specifically cytosines adjacent to guanosine nucleotides (CpG islands)...
  2. Rac GTPase signaling during embryonic salivary gland branching morphogenesis
    Sharon Sequeira; Fiscal Year: 2012
    ..basement membrane;ECM, extracellular matrix;GEF, guanine nucleotide exchange factor;GTPase, guanosine triphosphate hydroxylase;IB, immunoblotting;IF, immunofluorescence;IP, immunoprecipitation;Par, partitioning-..
  3. Peter Sarnow; Fiscal Year: 2015
    ..3 proposes to examine roles for the known isoforms of miR-122 that contain extra 3'terminal adenosine or guanosine residues, on HCV RNA abundance...
  4. Prevention of Inherited Retinal Diseases by Therapeutic Rare Earth Nanoparticles
    JAMES FRANCIS MCGINNIS; Fiscal Year: 2010
    ..with antibodies against products of ROS activity including acrolein, nitrotyrosine and 8-hydroxy-2-deoxy-guanosine. The effects of the nanoceria on neuroprotective pathways will be analyzed by Western blots, cDNA micro arrays, ..
  5. Biomarkers to Assess Selenium Chemoprevention for NSCLC
    Steven A Belinsky; Fiscal Year: 2012
    ..Our studies have identified genes inactivated by aberrant cytosine-guanosine (CpG) island methylation as candidate biomarkers for early detection of lung cancer...
  6. High-affinity RNA targets of Survival Motor Neuron Protein
    Ravindra N Singh; Fiscal Year: 2013
    ..SMN contains a distinct nucleic acid binding domain that has been shown to have preference for poly-guanosine (poly rG) RNAs in vitro...
  7. Carl W White; Fiscal Year: 2015
    ..SM analog CEES (2-chloroethyl ethylsulfide) caused inflammation, DNA damage (8-hydroxydeoxy-guanosine accumulation;comet assay;ATM/ATR activation and downstream signaling) and lipid peroxidation (4- hydroxynonenal) ..
  8. Peptide Therapy for Pulmonary Arterial Hypertension
    Jawaharlal M Patel; Fiscal Year: 2013
    ..of PH suggests endothelial dysfunction that causes diminished nitric oxide (NO) production and NO/cyclic guanosine 5'monophosphate (cGMP)- dependent vasorelaxation in the pulmonary circulation...
  9. Nancy J Brown; Fiscal Year: 2014
    ..by this grant over the last four years support the hypothesis that pharmacologic measures to increase cyclic guanosine monophosphate (cGMP) improve glucose homeostasis...
  10. Peter A Beal; Fiscal Year: 2016
    ..Since inosine is decoded as guanosine during translation, this modification can lead to changes in the meaning of codons (recoding)...
  11. Regulation and function of cGMP dependent protein kinase in cardiac hypertrophy
    Eiki Takimoto; Fiscal Year: 2013
    ..Cyclic guanosine-3', 5'-monophosphate (cGMP) is an intracellular second messenger which controls diverse cell ..
  12. JOY N JONES BUIE; Fiscal Year: 2014
    ..Finally, we will measure NO and cyclic guanosine monophosphate (cGMP) levels following IFN-alpha treatment to ascertain its impact on functional eNOS outputs...
  13. Soluble guanylyl cyclase modulation by endogenous elements and exogenous factors
    Emil Martin; Fiscal Year: 2012
    ..cyclase (sGC) - converts this extracellular NO signal into intracellular messenger 3'-5'cyclic guanosine monophosphate...
  14. Rac1 and Rac2 Guanosine Triphosphatases in Erythroid Function and Differentiation
    THEODOSIA ANASTASIOS KALFA; Fiscal Year: 2012
    The Rho guanosine triphosphatases (GTPases) Rac1 and Rac2 regulate actin cytoskeleton, microtubule dynamics, gene transcription, proliferation, and survival in multiple cell types...
  15. Michael Rosbash; Fiscal Year: 2016
    ..This enzyme deaminates adenosine to inosine, which is interpreted by the ribosomal machinery as a guanosine. ADAR edited substrates can be identified by sequencing RNA, i.e...
  16. DEVELOPING PET AGENTS FOR IMAGING PHOSPHODIESTERASE 10A (PDE10A)
    Zhude Tu; Fiscal Year: 2012
    ..medium-sized spiny projection neurons (MSN) where it plays a critical role in the regulation of both cyclic guanosine monophosphate (cGMP) and cyclic adenosine monophosphate (cAMP)...
  17. RNA Interference for the Treatment of Ebola and Marburg Hemorrhagic Fever
    Ian MacLachlan; Fiscal Year: 2013
    ..sufficient antiviral activity against filoviruses will be modified by substituting 2'-O-methyl (2'OMe) guanosine and/or uridines in the sense and antisense strands to eliminate the immune stimulatory capacity of the siRNA...
  18. JEFFREY SCOTT MUGRIDGE; Fiscal Year: 2014
    ..A critical step in this pathway is the removal of the protective methyl-guanosine cap found on the 5'-end of all eukaryotic mRNA, which commits the transcript to rapid degradation...
  19. Regulation of motility and organelle assembly at the Pseudomonas aeruginosa pole
    Barbara I Kazmierczak; Fiscal Year: 2012
    ..FimX is a phosphodiesterase for bis-(3'-5')-cyclic dimeric guanosine monophosphate (c-di-GMP), a second messenger implicated in the post-transcriptional control of many bacterial ..
  20. The Role of an Intraflagellar Transport Protein in Cell-Cycle Control
    Christopher R Wood; Fiscal Year: 2010
    ..Because sequence analysis of IFT27 reveals significant similarity to proteins of the Rab family of guanosine triphosphatases (GTPases), it is hypothesized that IFT27 may be functioning as a regulator of the cell cycle in ..
  21. Tomoaki Ogino; Fiscal Year: 2016
    ..In Aim 2, we will search for guanosine analogs that inhibit the formation of the functional cap structure by the VSV L protein, and establish high-..
  22. Stephen R Sprang; Fiscal Year: 2016
    ..Ric-8A is a Guanine nucleotide Exchange Factor (GEF) that catalyzes the exchange of guanosine diphosphate (GDP) for guanosine triphosphate (GTP) at the active site of G?...
  23. Angiotensin-II, GTPCH1 and 26S Protesomes
    Ming Hui Zou; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Guanosine 5'-triphosphate cyclohydrolase I (GTPCH1) is a homodecameric protein consisting of 25-kDa subunits, which enzyme catalyzes the rearrangement of GTP to dihydroneopterin triphosphate, a ..
  24. E. coli Growth Parameters in the Intestine
    Tyrrell Conway; Fiscal Year: 2010
    ..The stringent response to nutrient limitation involves the rapid accumulation of a unique nucleotide, guanosine 3', 5'-bispyrophosphate (ppGpp), which causes extensive reprogramming of gene expression during growth ..
  25. Zhude Tu; Fiscal Year: 2015
    ..medium-sized spiny projection neurons (MSN) where it plays a critical role in the regulation of both cyclic guanosine monophosphate (cGMP) and cyclic adenosine monophosphate (cAMP)...
  26. NO-independent cGMP regulation of vascular remodeling
    David A Tulis; Fiscal Year: 2012
    Vascular smooth muscle (VSM) cyclic guanosine 3',5'-moriophosphate (cGMP) serves as a critical regulator of many cellular functions that contribute to vessel growth after injury...
  27. Katherine L B Borden; Fiscal Year: 2016
    ..eIF4E functions in both the nucleus and cytoplasm. In the cytoplasm, it binds the 7-methyl guanosine (m7G) cap found on the 5'end of mRNAs thereby allowing translation initiation...
  28. Novel Peptide for Dynamic Regulation of Lung Endothelium NOS/cGMP Functions
    Jawaharlal M Patel; Fiscal Year: 2011
    ..Pulmonary vascular endothelial nitric oxide (NO) synthase (eNOS)- derived NO is the major stimulant of cyclic guanosine 5'monophosphate (cGMP) production and NO/cGMP-dependent vasorelaxation in the pulmonary circulation...
  29. EDWIN KERRY JACKSON; Fiscal Year: 2016
    DESCRIPTION (provided by applicant): Adenosine and guanosine are naturally-occurring and structurally similar purines...
  30. Effects of Arginase in Right Ventricular Hypertrophy and Failure
    S JULIE ANN LLOYD; Fiscal Year: 2012
    ..We believe that one potential mechanism of action involves signaling via cyclic guanosine monophosphate (cGMP) and nitric oxide (NO)...
  31. Donald M Kurtz; Fiscal Year: 2014
    ..causing epidemic cholerae, catalyzing formation and decay of the bacterial "second messenger", cyclic-di-guanosine monophosphate, are the focus in these latter two specific aims...
  32. GC-C Agonists: Specific Probes for the Detection of Colorectal Tumors
    Kunwar Shailubhai; Fiscal Year: 2013
    ..Binding of these peptide hormones to the GC-C receptor stimulates intracellular production of cyclic guanosine monophosphate (cGMP), which in turn regulates ion and water homeostasis within the gastrointestinal mucosa...
  33. Katherine L B Borden; Fiscal Year: 2016
    ..eIF4E functions in both the nucleus and cytoplasm. In the cytoplasm, it binds the 7 methyl guanosine (m7G) cap found on the 5'end of mRNAs thereby allowing translation initiation...
  34. HAVVA FITNAT YILDIZ; Fiscal Year: 2016
    ..In many bacteria including V. cholerae, cyclic dimeric guanosine monophosphate (c-diGMP) regulates motility, biofilm formation and virulence...
  35. Lei Wang; Fiscal Year: 2015
    ..Cyclic guanosine-monophosphate (GMP) is involved in the regulation of many neural functions...
  36. Gary A Piazza; Fiscal Year: 2016
    ..activity of a highly efficacious NSAID, sulindac, does not require the inhibition of COX-1 or -2 and that cyclic guanosine monophosphate phosphodiesterase (cGMP PDE) inhibition is responsible for its tumor cell growth inhibitory ..
  37. John C Burnett; Fiscal Year: 2015
    ..by the biology, therapeutics and diagnostics of the NPs and their GC receptors linked to 3',5'cyclic guanosine monophosphate (cGMP)...
  38. Horng H Chen; Fiscal Year: 2016
    ..interaction between diuretic therapy, the renin-angiotensin-aldosterone-system (RAAS) and cyclic 3_- 5_-guanosine monophosphate (cGMP) pathway...
  39. A High-Throughput Assay for Probes of the Flavivirus RNA Guanylyltransferase
    Brian Geiss; Fiscal Year: 2012
    ..The GTase transfers a guanosine monophosphate from GTP to the 5'end of the genomic RNA to produce the cap 0 structure...
  40. EMILY JOY TSAI; Fiscal Year: 2016
    ..mechanism by which the loss of caveolae- localized sGC contributes to the pathologic signaling of NO and cyclic guanosine monophosphate (cGMP) in cardiac hypertrophy...
  41. Vibrio cholerae c-diGMP signaling: motile to biofilm transition and transmission
    HAVVA FITNAT YILDIZ; Fiscal Year: 2012
    ..In many bacteria including V. cholerae, cyclic dimeric guanosine monophosphate (c-diGMP) regulates motility, biofilm formation and virulence...
  42. HAVVA FITNAT YILDIZ; Fiscal Year: 2016
    ..biofilm matrix components, the mechanisms and regulation of biofilm formation, the mechanism of cyclic dimeric guanosine monophosphate (c- diGMP) signaling, and their importance in the biology of V. cholerae...
  43. REBECCA LYNN WILLIAMS; Fiscal Year: 2014
    ..which may be predictive of successful prophylactic neuroprotection with CpG ODNs (unmethylated cytosine-guanosine rich oligodeoxynucleotides)...
  44. SHEILA SUE DAVID; Fiscal Year: 2016
    ..The new structures to be studied include amine adducts of oxidized guanosine. Novel pathways are proposed that might explain additional observations in the mutagenic spectrum of oxidative ..
  45. Regulatory networks involved in Mycobacterium tuberculosis persistence
    Petros C Karakousis; Fiscal Year: 2013
    ..We and others have shown that the alarmone hyperphosphorylated guanosine ((p)ppGpp) and the regulatory molecule inorganic polyphosphate (poly P) play a role in Mtb survival under growth-..
  46. REPERFUSION INJURY AND THE LECTIN COMPLEMENT PATHWAY
    CHARLES COLLARD; Fiscal Year: 2001
    ..of the terminal complement complex (C5b-9) on human endothelial cells decreases intracellular levels of cyclic guanosine monophosphate (cGMP) and increases cell surface expression of vascular cell adhesion molecules (VCAM-1) and ..
  47. Song Tan; Fiscal Year: 2016
    ..These fundamental processes are controlled by localizing the small Ran guanosine triphosphatase (GTPase) protein in its GDP or GTP bound state within the cytoplasm or the nucleus respectively, ..
  48. LINDA B MC GOWN; Fiscal Year: 2014
    ..DNA fragments in the second dimension of sequence using a new gel medium that is formed by self-association of guanosine compounds...
  49. GC-C agonists for colitis-induced CRC
    Kunwar Shailubhai; Fiscal Year: 2009
    ..lumen of the GI tract where they bind to Guanylate Cyclase C (GC-C), resulting in enhanced production of cyclic guanosine monophosphate (cGMP) and activation of cystic fibrosis transmembrane conductance regulator (CFTR)...
  50. Chemistry of Reactive Intermediates Generated from Benzothiazole Derived Drug Can
    Michael Novak; Fiscal Year: 2009
    ..3. To study the reaction of the cations with cellular nucleophiles, particularly guanosine, which is known to be the main biological target of nitrenium ions derived from carcinogenic aromatic amines, ..
  51. AFLATOXIN DNA ADDUCTS DETECTED BY MONOCLONAL ANTIBODIES
    John Groopman; Fiscal Year: 1992
    ..A) A novel DNA adduct-oxime antigen and (B) a hapten made from aflatoxin guanosine adduct produced by synthetic aflatoxin epoxide will be tried to induce an antibody specificity and affinity for ..
  52. NEUROFIBROMATOSIS TYPE 1 GENE REGULATES MYELOPOIESIS
    DAVID CLAPP; Fiscal Year: 2001
    ..p21ras (Ras) family of protooncogenes control cellular growth and differentiation by cycling between an active guanosine triphosphate (GTP)-bound state and an inactive guanosine diphosphate (GDP)-bound state...
  53. REGULATION OF GLCNAC-PI DEACETYLATION
    Victoria Stevens; Fiscal Year: 1999
    ..This effect is specific for the guanosine-containing nucleotide triphosphate and occurs at a concentration of GTP (optimal at 1 mM) that is ..
  54. ALTERNATIVE SIGMA FACTORS AND ANTIBIOTIC SYNTHESIS
    GEORGE JONES; Fiscal Year: 1999
    ..antibioticus; (6)examination of the role of guanosine tetraphosphate in regulating the expression of the sigE gene; and (7)identification of other genes in S...
  55. Medical Management of Pediatric Neurotransmitter Disorders- A Multidisciplinary
    K Michael Gibson; Fiscal Year: 2007
    ..those that will be discussed at this conference include: aromatic L- amino acid decarboxylase deficiency (AADC), guanosine triphosphate cyclohydrolase deficiency (Segawa's disease or DOPA-responsive dystonia, GTPCH), tyrosine ..
  56. Agonists of guanylate cyclase receptor for colon cancer
    Kunwar Shailubhai; Fiscal Year: 2002
    ..we believe uroguanylin binds to guanylate cyclase C (GC-C) and stimulates intracellular production of cyclic guanosine monophosphate (cGMP), resulting in activation of cystic fibrosis transmembrane conductance regulator (CFTR)...
  57. BASIS FOR RENAL RESISTANCE TO ATRIAL NATRIURETIC PEPTIDE
    Michael Humphreys; Fiscal Year: 2004
    ..PDE) enzyme in renal target cells for ANP action such that ANFs intracellular second messenger cyclic guanosine-3',5'-monophosphate (cGMP), normally formed after ANP binds to its biologically active receptors, is rapidly ..
  58. NO AND THE MAGNOCELLULAR NEUROENDOCRINE SYSTEM
    MASSAKO KADEKARO KUTYNA; Fiscal Year: 2002
    ..NO synthesized locally in the SON during osmotic stimulation activates guanylyl cyclase to increase 3',5' cyclic guanosine monophosphate (cGMP) which results in 2) inhibition of angiotensin II and prostaglandin release in the SON to ..
  59. A Novel HTS Cell-Based Assay For Cyclic GMP
    Gary Piazza; Fiscal Year: 2007
    unreadable] DESCRIPTION (provided by applicant): Cyclic guanosine monophosphate (cGMP) plays an essential role in signal transduction to regulate a diverse range of cellular processes, including tumor cell survival...
  60. ANTIVIRAL THERAPY WITH N-DOCOSANOL PLUS ACYCLOVIR
    LAURA POPE; Fiscal Year: 2000
    ..The guanosine analog acyclovir remains a leading approved drug for the treatment of oral and genital HSV diseases...
  61. Ribonucleotide monophosphates and umami taste
    Eugene Delay; Fiscal Year: 2003
    ..will define some of the taste qualities of the 5' ribonucleotide monophosphates, inosine monophosphate (IMP) and guanosine monophosphate (GMP) that are important substances used to study "umami" taste...
  62. Cloning, expression and purification of uroguanylin
    Kunwar Shailubhai; Fiscal Year: 2002
    ..we believe UG binds to guanylate cyclase receptors (GC-C) and stimulates intracellular production of cyclic guanosine monophosphate (cGMP), resulting in activation of cystic fibrosis transmembrane conductance regulator (CFTR)...
  63. Biochemical System for Study of DNA Repair
    Weiguo Cao; Fiscal Year: 2003
    ..DNA deamination results in conversion of adenosine to inosine, cytosine to uridine, and guanosine to xanthosine. Endonuclease V mediates an evolutionary conserved pathway involved in inosine repair...
  64. METABOLISM OF GUANOSINE IN ISCHEMIC MYOCARDIUM
    TIMOTHY GEISBUHLER; Fiscal Year: 1991
    Little is known about the mechanisms by which guanosine and guanine nucleotide metabolism is controlled in the heart, or of the contribution of these compounds to myocardial cell function...
  65. STRUCTURE AND DYNAMICS OF APTAMERS AND TELOMERE DNAS
    PHILIP BOLTON; Fiscal Year: 1999
    ..This DNA aptamer exhibits a new structural motif which consists of a "chair" like overall fold with tetrads of guanosine base pairs and three loops...