Summary: A nucleoside that substitutes for thymidine in DNA and thus acts as an antimetabolite. It causes breaks in chromosomes and has been proposed as an antiviral and antineoplastic agent. It has been given orphan drug status for use in the treatment of primary brain tumors.

Top Publications

  1. ncbi Doublecortin expression levels in adult brain reflect neurogenesis
    Sebastien Couillard-Despres
    Volkswagen Foundation Junior Group, University of Regensburg, Franz Josef Strauss Allee 11, 93053 Regensburg, Germany
    Eur J Neurosci 21:1-14. 2005
  2. ncbi Neurogenesis in the adult is involved in the formation of trace memories
    T J Shors
    Department of Psychology and Center for Collaborative Neuroscience, Rutgers University, Piscataway, New Jersey 08854, USA
    Nature 410:372-6. 2001
  3. ncbi Transient expression of doublecortin during adult neurogenesis
    Jason P Brown
    Department of Neurology, University of Regensburg, 93053 Regensburg, Germany
    J Comp Neurol 467:1-10. 2003
  4. ncbi The utility of Ki-67 and BrdU as proliferative markers of adult neurogenesis
    N Kee
    Department of Physiology, Medical Sciences Building, University of Toronto, Ont, Canada M5S 1A8
    J Neurosci Methods 115:97-105. 2002
  5. ncbi Infusion of brain-derived neurotrophic factor into the lateral ventricle of the adult rat leads to new neurons in the parenchyma of the striatum, septum, thalamus, and hypothalamus
    V Pencea
    Department of Cell Biology, Emory University School of Medicine, Atlanta, Georgia 30322, USA
    J Neurosci 21:6706-17. 2001
  6. ncbi Neurogenesis in the neocortex of adult primates
    E Gould
    Department of Psychology, Princeton University, Princeton, NJ 08544, USA
    Science 286:548-52. 1999
  7. pmc Analysis of histone 2B-GFP retention reveals slowly cycling hematopoietic stem cells
    Adlen Foudi
    Cancer Center, Massachusetts General Hospital, Harvard Medical School, 185 Cambridge Street, Boston, Massachusetts 02114, USA
    Nat Biotechnol 27:84-90. 2009
  8. ncbi Maturation and death of adult-born olfactory bulb granule neurons: role of olfaction
    Leopoldo Petreanu
    The Rockefeller University, New York, New York 10021, USA
    J Neurosci 22:6106-13. 2002
  9. ncbi A role for adult neurogenesis in spatial long-term memory
    J S Snyder
    Department of Physiology, University of Toronto, 1 King s College Circle, Room 3214, Toronto, Ontario, Canada M5S 1A8
    Neuroscience 130:843-52. 2005
  10. ncbi BrdU immunohistochemistry for studying adult neurogenesis: paradigms, pitfalls, limitations, and validation
    Philippe Taupin
    National Neuroscience Institute, Singapore
    Brain Res Rev 53:198-214. 2007

Detail Information

Publications323 found, 100 shown here

  1. ncbi Doublecortin expression levels in adult brain reflect neurogenesis
    Sebastien Couillard-Despres
    Volkswagen Foundation Junior Group, University of Regensburg, Franz Josef Strauss Allee 11, 93053 Regensburg, Germany
    Eur J Neurosci 21:1-14. 2005
  2. ncbi Neurogenesis in the adult is involved in the formation of trace memories
    T J Shors
    Department of Psychology and Center for Collaborative Neuroscience, Rutgers University, Piscataway, New Jersey 08854, USA
    Nature 410:372-6. 2001
    ..These results indicate that newly generated neurons in the adult are not only affected by the formation of a hippocampal-dependent memory, but also participate in it...
  3. ncbi Transient expression of doublecortin during adult neurogenesis
    Jason P Brown
    Department of Neurology, University of Regensburg, 93053 Regensburg, Germany
    J Comp Neurol 467:1-10. 2003
    ..Adult neurogenesis is typically detected by incorporation of bromodeoxyuridine (BrdU) into dividing cells and colabeling of BrdU-positive cells with markers for mature neurons...
  4. ncbi The utility of Ki-67 and BrdU as proliferative markers of adult neurogenesis
    N Kee
    Department of Physiology, Medical Sciences Building, University of Toronto, Ont, Canada M5S 1A8
    J Neurosci Methods 115:97-105. 2002
    ..Thus, Ki-67 is an effective mitotic marker and has most of the benefits of BrdU and none of the costs. This study provides evidence for Ki-67 to be used as a marker of proliferation in the initial phase of adult neurogenesis...
  5. ncbi Infusion of brain-derived neurotrophic factor into the lateral ventricle of the adult rat leads to new neurons in the parenchyma of the striatum, septum, thalamus, and hypothalamus
    V Pencea
    Department of Cell Biology, Emory University School of Medicine, Atlanta, Georgia 30322, USA
    J Neurosci 21:6706-17. 2001
    ..16 d after intraventricular administration of BDNF in conjunction with the cell proliferation marker bromodeoxyuridine (BrdU) for 12 d...
  6. ncbi Neurogenesis in the neocortex of adult primates
    E Gould
    Department of Psychology, Princeton University, Princeton, NJ 08544, USA
    Science 286:548-52. 1999
    ..These new neurons, which are continually added in adulthood, may play a role in the functions of association neocortex...
  7. pmc Analysis of histone 2B-GFP retention reveals slowly cycling hematopoietic stem cells
    Adlen Foudi
    Cancer Center, Massachusetts General Hospital, Harvard Medical School, 185 Cambridge Street, Boston, Massachusetts 02114, USA
    Nat Biotechnol 27:84-90. 2009
    ..targeted at rapidly cycling cells and have been presumed to retain labels such as the thymidine analog 5-bromodeoxyuridine (BrdU). However, BrdU retention is neither a sensitive nor specific marker for HSCs...
  8. ncbi Maturation and death of adult-born olfactory bulb granule neurons: role of olfaction
    Leopoldo Petreanu
    The Rockefeller University, New York, New York 10021, USA
    J Neurosci 22:6106-13. 2002
    ..This selection mechanism might allow the construction of specific OB circuits based on olfactory experience and suggests possible functions of OB cell replacement...
  9. ncbi A role for adult neurogenesis in spatial long-term memory
    J S Snyder
    Department of Physiology, University of Toronto, 1 King s College Circle, Room 3214, Toronto, Ontario, Canada M5S 1A8
    Neuroscience 130:843-52. 2005
    ..These results suggest a new role for adult neurogenesis in the formation and/or consolidation of long-term, hippocampus-dependent, spatial memories...
  10. ncbi BrdU immunohistochemistry for studying adult neurogenesis: paradigms, pitfalls, limitations, and validation
    Philippe Taupin
    National Neuroscience Institute, Singapore
    Brain Res Rev 53:198-214. 2007
    b>Bromodeoxyuridine (BrdU) is a thymidine analog that incorporates DNA of dividing cells during the S-phase of the cell cycle. As such, BrdU is used for birth dating and monitoring cell proliferation...
  11. ncbi Evidence that brain-derived neurotrophic factor is required for basal neurogenesis and mediates, in part, the enhancement of neurogenesis by dietary restriction in the hippocampus of adult mice
    Jaewon Lee
    Laboratory of Neurosciences, National Institute on Aging Gerontology Research Center, Baltimore, Maryland 21224, USA
    J Neurochem 82:1367-75. 2002
    ..Mice were then injected with bromodeoxyuridine (BrdU) and killed either 1 day or 4 weeks later...
  12. pmc Neurogenesis in dentate subgranular zone and rostral subventricular zone after focal cerebral ischemia in the rat
    K Jin
    Buck Institute for Age Research, Novato, CA 94945, USA
    Proc Natl Acad Sci U S A 98:4710-5. 2001
    ..These results support a role for ischemia-induced neurogenesis in what may be adaptive processes that contribute to recovery after stroke...
  13. ncbi GABAergic excitation promotes neuronal differentiation in adult hippocampal progenitor cells
    Yusuke Tozuka
    Department of Integrated Biosciences, University of Tokyo, Kashiwa 277 8562, Japan
    Neuron 47:803-15. 2005
    ..Thus, it appears that GABAergic inputs to hippocampal progenitor cells promote activity-dependent neuronal differentiation...
  14. ncbi Male pheromone-stimulated neurogenesis in the adult female brain: possible role in mating behavior
    Gloria K Mak
    Hotchkiss Brain Institute, Department of Cell Biology and Anatomy, Faculty of Medicine, University of Calgary, Calgary, Alberta T2N 4N1, Canada
    Nat Neurosci 10:1003-11. 2007
    ..These results suggest that male pheromones are involved in regulating neurogenesis in both the olfactory bulb and hippocampus, which may be important for female reproductive success...
  15. pmc Newly generated neurons in the amygdala and adjoining cortex of adult primates
    Patrick J Bernier
    Centre de Recherche Universite Laval Robert Giffard, 2601, chemin de la Canardiere, Local F 6500, Beauport, QC, Canada G1J 2G3
    Proc Natl Acad Sci U S A 99:11464-9. 2002
    ..These two concomitant phenomena may ensure structural stability and functional plasticity to the primate olfactory system and temporal lobe...
  16. ncbi Neural stem cell detection, characterization, and age-related changes in the subventricular zone of mice
    Alexander Y Maslov
    Department of Molecular and Cellular Biology, Roswell Park Cancer Institute, Buffalo, New York 14263, USA
    J Neurosci 24:1726-33. 2004
    ..The reduction in the neural stem cell population documented here is sufficient to account for the reduced level of neurogenesis in old animals...
  17. ncbi Proliferation and differentiation of progenitor cells throughout the intact adult rat spinal cord
    P J Horner
    The Salk Institute for Biological Studies, Laboratory of Genetics, La Jolla, California 92037, USA
    J Neurosci 20:2218-28. 2000
    ..b>Bromodeoxyuridine (BrdU) was used to label dividing cells in 13- to 14-week-old, intact Fischer rats...
  18. ncbi Adult neurogenesis produces a large pool of new granule cells in the dentate gyrus
    H A Cameron
    Laboratory of Molecular Biology, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland 20892 USA
    J Comp Neurol 435:406-17. 2001
    ..The S-phase marker bromodeoxyuridine (BrdU) has been extensively used in recent studies of adult neurogenesis, but it has been carefully tested ..
  19. ncbi Expression and function of orphan nuclear receptor TLX in adult neural stem cells
    Yanhong Shi
    Gene Expression Laboratory, The Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, California 92037, USA
    Nature 427:78-83. 2004
    ..TLX can silence glia-specific expression of the astrocyte marker GFAP in neural stem cells, suggesting that transcriptional repression may be crucial in maintaining the undifferentiated state of these cells...
  20. ncbi Label-retaining epithelial cells in mouse mammary gland divide asymmetrically and retain their template DNA strands
    Gilbert H Smith
    Laboratory of Mammary Gland Biology and Tumorigenesis, Center for Cancer Research, National Cancer Institute, Bethesda, MD 20892, USA
    Development 132:681-7. 2005
    ..Labeling newly synthesized DNA in these glands with a different marker, 5-bromodeoxyuridine (5BrdU), resulted in the appearance of doubly labeled nuclei in a large percentage of the LREC...
  21. ncbi Chronic olanzapine or fluoxetine administration increases cell proliferation in hippocampus and prefrontal cortex of adult rat
    Masafumi Kodama
    Division of Molecular Psychiatry, Department of Psychiatry, Yale University School of Medicine, New Haven, Connecticut 06508, USA
    Biol Psychiatry 56:570-80. 2004
    ..There has been increasing evidence that atypical antipsychotics are effective in the treatment of mood disorders or for augmenting 5-hydroxytryptamine selective reuptake inhibitors for treatment-resistant depression...
  22. ncbi Increased neurogenesis in a model of electroconvulsive therapy
    T M Madsen
    Neuropsychiatry Laboratory, Department of Psychiatry, H S Rigshospitalet, TMM, TGB, Copenhagen, Denmark
    Biol Psychiatry 47:1043-9. 2000
    ..Past research has shown seizure activity to be a regulator of neurogenesis in the adult brain. This study examines the effect of a single and multiple electroconvulsive seizures on neurogenesis in the rat dentate gyrus...
  23. ncbi A critical role for histone H2AX in recruitment of repair factors to nuclear foci after DNA damage
    T T Paull
    Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892 0540, USA
    Curr Biol 10:886-95. 2000
    ..Recently it has been reported that a member of the histone H2A family, H2AX, becomes extensively phosphorylated within 1-3 minutes of DNA damage and forms foci at break sites...
  24. pmc Different effects of bromodeoxyuridine and [3H]thymidine incorporation into DNA on cell proliferation, position, and fate
    Alvaro Duque
    Department of Neurobiology, Kavli Institute for Neuroscience, Yale University School of Medicine, New Haven, Connecticut 06510, USA
    J Neurosci 31:15205-17. 2011
    As markers of DNA synthesis, [(3)H]thymidine ([(3)H]dT) and the later developed analog bromodeoxyuridine (BrdU) have revolutionized our ability to identify dividing cells and follow their fate in various tissues, including the nervous ..
  25. ncbi Irradiation induces neural precursor-cell dysfunction
    Michelle L Monje
    Department of Neurosurgery, Stanford University, Stanford, California, USA
    Nat Med 8:955-62. 2002
    ..These findings provide clear targets for future therapeutic interventions...
  26. ncbi Bromodeoxyuridine specifically labels the regenerative stem cells of planarians
    P A Newmark
    Department of Embryology, Carnegie Institution of Washington, 115 West University Parkway, Baltimore, Maryland 21210, USA
    Dev Biol 220:142-53. 2000
    ..Here we show that planarians are, in fact, capable of incorporating the thymidine analogue bromodeoxyuridine (BrdU), allowing neoblasts to be labeled specifically during the S phase of the cell cycle...
  27. ncbi Long-term fate and distribution of newborn cells in the adult mouse olfactory bulb: Influences of olfactory deprivation
    N Mandairon
    Laboratoire de Neurosciences et Systèmes Sensoriels, CNRS UMR 5020, Universite Claude Bernard Lyon 1, 50 avenue Tony Garnier, 6936, Lyon Cedex 07, France
    Neuroscience 141:443-51. 2006
    ..Using bromodeoxyuridine labeling, the survival of newborn cells was assessed in these two layers of the MOB in control mice and in ..
  28. ncbi Leukemia inhibitory factor promotes neural stem cell self-renewal in the adult brain
    Sylvian Bauer
    Biology Division, California Institute of Technology, Pasadena, California 91125, USA
    J Neurosci 26:12089-99. 2006
    ..Our results have implications for the development of therapeutic strategies for brain repair and suggest that LIF may be useful, in combination with other factors, in promoting regeneration in the adult brain...
  29. pmc High incidence of non-random template strand segregation and asymmetric fate determination in dividing stem cells and their progeny
    Michael J Conboy
    Department of Neurology and Neurological Sciences, Stanford University School of Medicine, Stanford, California, United States of America corrected
    PLoS Biol 5:e102. 2007
    ..Using sequential pulses of halogenated thymidine analogs (bromodeoxyuridine [BrdU], chlorodeoxyuridine [CldU], and iododeoxyuridine [IdU]), and analyzing stem cell progeny during ..
  30. ncbi Interneurons produced in adulthood are required for the normal functioning of the olfactory bulb network and for the execution of selected olfactory behaviors
    Vincent Breton-Provencher
    Cellular Neurobiology Unit, Centre de Recherche Universite Laval Robert Giffard, Quebec, Quebec G1J 2G3, Canada
    J Neurosci 29:15245-57. 2009
    ..Altogether, our data highlight the importance of adult neurogenesis for the proper functioning of the OB network and imply that new bulbar interneurons are involved in some, but not all, odor-associated tasks...
  31. ncbi Roles of continuous neurogenesis in the structural and functional integrity of the adult forebrain
    Itaru Imayoshi
    Institute for Virus Research, Kyoto University, Shogoin Kawahara, Sakyo ku, Kyoto 606 8507, Japan
    Nat Neurosci 11:1153-61. 2008
  32. ncbi Reduced hippocampal neurogenesis and number of hilar neurones in streptozotocin-induced diabetic mice: reversion by antidepressant treatment
    Juan Beauquis
    Laboratory of Neuroendocrine Biochemistry, Institute of Biology and Experimental Medicine, National Research Council Argentina, Obligado 2490 1428 Buenos Aires, Argentina
    Eur J Neurosci 23:1539-46. 2006
    ..In conclusion, fluoxetine treatment restored neuroplasticity-related hippocampal alterations of diabetic mice. These findings may be potentially important to counteract diabetes-associated depression in humans...
  33. ncbi Integration and sensory experience-dependent survival of newly-generated neurons in the accessory olfactory bulb of female mice
    L Oboti
    Department of Animal and Human Biology, Via Accademia Albertina 13, Turin, Italy
    Eur J Neurosci 29:679-92. 2009
    ..These included fate mapping of bromodeoxyuridine-labelled cells, lineage tracing of SVZ-derived enhanced green fluorescent protein-positive engrafted cells ..
  34. ncbi The adult substantia nigra contains progenitor cells with neurogenic potential
    D Chichung Lie
    Laboratory of Genetics, The Salk Institute for Biological Studies, La Jolla, California 92037, USA
    J Neurosci 22:6639-49. 2002
    ..This developmental potential of SN progenitor cells might be useful for future endogenous cell replacement strategies in Parkinson's disease...
  35. pmc Stem cell proliferation and quiescence--two sides of the same coin
    Ingmar Glauche
    Institute for Medical Informatics, Statistics and Epidemiology, University of Leipzig, Leipzig, Germany
    PLoS Comput Biol 5:e1000447. 2009
    The kinetics of label uptake and dilution in dividing stem cells, e.g., using Bromodeoxyuridine (BrdU) as a labeling substance, are a common way to assess the cellular turnover of all hematopoietic stem cells (HSCs) in vivo...
  36. ncbi Gender and endogenous levels of estradiol do not influence adult hippocampal neurogenesis in mice
    Diane C Lagace
    Department of Psychiatry, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA
    Hippocampus 17:175-80. 2007
    ..These findings underscore a distinct regulation of adult neurogenesis in mice vs. other rodents, and are discussed in regard to their implications for the study of adult hippocampal neurogenesis...
  37. pmc BLM helicase-dependent transport of p53 to sites of stalled DNA replication forks modulates homologous recombination
    Sagar Sengupta
    Laboratory of Human Carcinogenesis, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    EMBO J 22:1210-22. 2003
    ..These results indicate that p53 and BLM functionally interact during resolution of stalled DNA replication forks and provide insight into the mechanism of genomic fidelity maintenance by these nuclear proteins...
  38. ncbi In vivo analysis of oligodendrocyte lineage development in postnatal FGF2 null mice
    Joshua C Murtie
    Program in Molecular and Cell Biology, Uniformed Services University of the Health Sciences, Bethesda, Maryland 20814 4799, USA
    Glia 49:542-54. 2005
    ..This in vivo analysis demonstrates that the predominant role of endogenous FGF2 on OLCs in development is inhibition of differentiation...
  39. pmc Identification of DNA-synthesizing bacterial cells in coastal North Sea plankton
    Annelie Pernthaler
    Max Planck Institute for Marine Microbiology, Bremen, Germany
    Appl Environ Microbiol 68:5728-36. 2002
    ..After incubation with the halogenated thymidine analogue bromodeoxyuridine (BrdU), environmental bacteria were identified by fluorescence in situ hybridization (FISH) with horseradish ..
  40. ncbi Turnover rates of B cells, T cells, and NK cells in simian immunodeficiency virus-infected and uninfected rhesus macaques
    Rob J de Boer
    Theoretical Biology, Utrecht University, Utrecht, The Netherlands
    J Immunol 170:2479-87. 2003
    ..Because the viral load and the CD4 T cell count are negatively correlated we cannot determine which of the two plays the most important role in this generalized immune activation...
  41. ncbi Disruption of ErbB receptor signaling in adult non-myelinating Schwann cells causes progressive sensory loss
    Suzhen Chen
    Division of Neuroscience, Children s Hospital, Harvard Medical School, 300 Longwood Avenue, Boston, Massachusetts 02115, USA
    Nat Neurosci 6:1186-93. 2003
  42. pmc The hepatic stem cell niche: identification by label-retaining cell assay
    Reiichiro Kuwahara
    Department of Medicine, Division of Digestive Diseases, Beth Israel Medical Center Albert Einstein College of Medicine, New York, NY 10003, USA
    Hepatology 47:1994-2002. 2008
    ..We used a sublethal dose of acetaminophen administered coincident with bromodeoxyuridine to load possible hepatic stem cells in mice with label and then administered a second, sublethal chase of ..
  43. ncbi Intestinal stem cells protect their genome by selective segregation of template DNA strands
    Christopher S Potten
    Epithelial Biology Department, Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Wilmslow Road, Manchester M9 4BX, UK
    J Cell Sci 115:2381-8. 2002
    ..Labelling newly synthesised strands with a different marker (bromodeoxyuridine, BrdUrd) allows segregation of the two markers to be studied...
  44. pmc Determination of key aspects of precursor cell proliferation, cell cycle length and kinetics in the adult mouse subgranular zone
    C D Mandyam
    Department of Psychiatry, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390 9070, USA
    Neuroscience 146:108-22. 2007
    ..key information is lacking about these proliferating SGZ precursors, from the fundamental--what dose of bromodeoxyuridine (BrdU) is appropriate for labeling all S phase cells?--to the detailed--what are the kinetics of BrdU-..
  45. pmc Diminished adult neurogenesis in the marmoset brain precedes old age
    Benedetta Leuner
    Department of Psychology, Princeton University, Princeton, NJ 08544, USA
    Proc Natl Acad Sci U S A 104:17169-73. 2007
    ..These data demonstrate that a substantial decrease in neurogenesis occurs before the onset of old age in the adult marmoset brain, suggesting the possibility that similar alterations occur in the human brain...
  46. pmc The effects of exercise and stress on the survival and maturation of adult-generated granule cells
    Jason S Snyder
    Unit on Neuroplasticity, Mood and Anxiety Disorders Program, National Institute of Mental Health, National Institutes of Health, Bethesda, MD 20892, USA
    Hippocampus 19:898-906. 2009
    ..Published 2009 Wiley-Liss, Inc...
  47. pmc Replicon clusters are stable units of chromosome structure: evidence that nuclear organization contributes to the efficient activation and propagation of S phase in human cells
    D A Jackson
    Sir William Dunn School of Pathology, University of Oxford, Oxford, OX1 3RE, United Kingdom
    J Cell Biol 140:1285-95. 1998
    ..HeLa cells were labeled with 5-bromodeoxyuridine (BrdU) at different times of S phase...
  48. ncbi Task difficulty in the Morris water task influences the survival of new neurons in the dentate gyrus
    Jonathan R Epp
    Department of Psychology, Graduate Program in Neuroscience, Brain Research Centre, University of British Columbia, Vancouver, British Columbia, Canada
    Hippocampus 20:866-76. 2010
    ..In conclusion, different types of hippocampus-dependent learning can differentially impact cell survival...
  49. ncbi A comparative analysis of intraperitoneal versus intracerebroventricular administration of bromodeoxyuridine for the study of cell proliferation in the adult rat brain
    M Cifuentes
    Departamento de Biologia Celular, Genética y Fisiología, Facultad de Ciencias, Universidad de Malaga, 29071 Malaga, Spain
    J Neurosci Methods 201:307-14. 2011
    b>Bromodeoxyuridine (BrdU) is the most widely used marker to detect proliferative cells in the adult brain. Here we analyse whether the route of administration of the tracer influences the number of labelled cells...
  50. ncbi Temporally specific burst in cell proliferation increases hippocampal neurogenesis in protracted abstinence from alcohol
    Kimberly Nixon
    Bowles Center for Alcohol Studies, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    J Neurosci 24:9714-22. 2004
    ..For the first time, these data provide a neurobiological mechanism that may underlie the return of human cognitive function and brain volume associated with recovery from addiction...
  51. ncbi Use of bromodeoxyuridine immunocapture to identify psychrotolerant phenanthrene-degrading bacteria in phenanthrene-enriched polluted Baltic Sea sediments
    Anna Edlund
    Sodertorn University College, School of Life Sciences, Huddinge, Sweden
    FEMS Microbiol Ecol 65:513-25. 2008
    ..PAH)-contaminated sediments were spiked with phenanthrene and incubated for 2 months in the presence of bromodeoxyuridine that is incorporated into the DNA of replicating cells...
  52. ncbi Widespread defects in the primary olfactory pathway caused by loss of Mash1 function
    Richard C Murray
    Department of Anatomy and Neurobiology, Developmental Biology Center, University of California, Irvine, Irvine, California 92697 2300, USA
    J Neurosci 23:1769-80. 2003
  53. pmc dNTP pools determine fork progression and origin usage under replication stress
    Jérôme Poli
    Institute of Human Genetics, CNRS UPR 1142, Montpellier, France
    EMBO J 31:883-94. 2012
    ..Since upregulation of RNR promotes fork progression in the presence of DNA lesions, we propose that CIN mutants adapt to chronic replication stress by upregulating dNTP pools...
  54. pmc Targeted deletion of ERK5 MAP kinase in the developing nervous system impairs development of GABAergic interneurons in the main olfactory bulb and behavioral discrimination between structurally similar odorants
    Junhui Zou
    Department of Environmental and Occupational Health Sciences, University of Washington, Seattle, WA 98195 7234, USA
    J Neurosci 32:4118-32. 2012
    ..We conclude that ERK5 is a novel signaling pathway regulating developmental OB neurogenesis and olfactory behavior...
  55. ncbi IGF-I synergizes with FGF-2 to stimulate oligodendrocyte progenitor entry into the cell cycle
    F Jiang
    Department of Neuroscience and Anatomy, Pennsylvania State University College of Medicine, Hershey, Pennsylvania 17033, USA
    Dev Biol 232:414-23. 2001
    ..Instead, IGF-I promoted increased recruitment of O-2A progenitors into the S phase of the cell cycle. These studies support a role for IGF-I as a cell cycle progression factor for progenitor cells...
  56. ncbi In vivo expansion of the mammary stem/ progenitor cell population by xanthosine infusion
    Anthony V Capuco
    Bovine Functional Genomics Laboratory, USDA ARS, Beltsville, MD 20705
    Exp Biol Med (Maywood) 234:475-82. 2009
    ..This is the first demonstration of an in vivo treatment to increase the endogenous population of mammary stem cells, with utility for biomedical research and dairy management...
  57. ncbi Identification of label-retaining cells in mouse endometrium
    Rachel W S Chan
    Centre for Women s Health Research, Monash University Department of Obstetrics and Gynaecology, Monash Medical Centre, Clayton, Vic, Australia
    Stem Cells 24:1529-38. 2006
    ..Endometrium was labeled for 3 days with bromodeoxyuridine (BrdU) in postnatal day 3 (P3) mice prior to gland development and prepubertal (P19) mice after glands had ..
  58. ncbi Cell proliferation without neurogenesis in adult primate neocortex
    D R Kornack
    Department of Neurobiology, Yale University School of Medicine, New Haven, CT 06510, USA
    Science 294:2127-30. 2001
    ..neurogenesis in adult macaques by using immunofluorescent triple labeling for the DNA-replication indicator, bromodeoxyuridine (BrdU), and neuronal and glial cell markers...
  59. pmc IGF-1 partially restores chemotherapy-induced reductions in neural cell proliferation in adult C57BL/6 mice
    Department of Radiation Oncology, University of Rochester Medical Center, New York 14642, USA
    Cancer Invest 28:544-53. 2010
    ..Insulin-like growth factor 1, a molecule implicated in promoting neurogenesis, counteracted the effects of high doses of chemotherapy on neural cell proliferation...
  60. ncbi Spatial learning affects immature granule cell survival in adult rat dentate gyrus
    P Ambrogini
    Institute of Physiological Sciences, University of Urbino, I 61029, Urbino, Italy
    Neurosci Lett 286:21-4. 2000
  61. ncbi Arrested neuronal proliferation and impaired hippocampal function following fractionated brain irradiation in the adult rat
    T M Madsen
    Laboratory of Neuropsychiatry, Department of Psychiatry O 6102, H S Rigshospitalet, Blegdamsvej 9, 2100, Copenhagen, Denmark
    Neuroscience 119:635-42. 2003
  62. ncbi Lmx1a and lmx1b function cooperatively to regulate proliferation, specification, and differentiation of midbrain dopaminergic progenitors
    Carol H Yan
    Department of Developmental Neurobiology, National Institute for Medical Research, The Ridgeway, London NW7 1AA, United Kingdom
    J Neurosci 31:12413-25. 2011
    ..Together, our results demonstrate that Lmx1a and Lmx1b function cooperatively to regulate proliferation, specification, and differentiation of mDA progenitors, including their floor plate-like properties...
  63. ncbi Enriched odor exposure increases the number of newborn neurons in the adult olfactory bulb and improves odor memory
    Christelle Rochefort
    Perception and Memory Laboratory, Centre National de la Recherche Scientifique Unité de Recherche Associée 2182, Institut Pasteur, 75 724 Paris Cedex 15, France
    J Neurosci 22:2679-89. 2002
    ..housed in either a standard or an odor-enriched environment for 40 d were injected intraperitoneally with bromodeoxyuridine (BrdU) to detect proliferation among progenitor cells and to follow their survival in the MOB...
  64. pmc Maturation time of new granule cells in the dentate gyrus of adult macaque monkeys exceeds six months
    Shawn J Kohler
    Neuroscience Program, Beckman Institute, University of Illinois, Urbana, IL 61801, USA
    Proc Natl Acad Sci U S A 108:10326-31. 2011
  65. ncbi Effects of prenatal immune activation on hippocampal neurogenesis in the rat
    Ke Cui
    Department of Psychiatry and of Neurology and Neurosurgery, McGill University, Douglas Mental Health University Institute, Montreal, Quebec, Canada
    Schizophr Res 113:288-97. 2009
    ..This indicates that deficits in neurogenesis caused by prenatal LPS are not mediated by LPS-induced fever or eicosanoid induction, but could be mediated by LPS-induced increases in maternal cytokines or corticosterone...
  66. pmc Regional heterogeneity in astrocyte responses following contusive spinal cord injury in mice
    Robin E White
    Neuroscience Graduate Studies Program, Ohio State University, Columbus, Ohio 43210, USA
    J Comp Neurol 518:1370-90. 2010
    ..b>Bromodeoxyuridine (BrdU) was administered to mice 3 days after SCI, and cells expressing BrdU and the astrocyte marker, glial ..
  67. pmc New markers for murine memory B cells that define mutated and unmutated subsets
    Shannon M Anderson
    Section of Immunobiology, Yale University School of Medicine, New Haven, CT 06510, USA
    J Exp Med 204:2103-14. 2007
    ..The apparent heterogeneity in the memory compartment implies either different origins and/or different functions, which we discuss...
  68. ncbi Electroconvulsive seizure treatment increases cell proliferation in rat frontal cortex
    Torsten M Madsen
    Laboratory of Molecular Psychiatry, Departments of Psychiatry and Pharmacology, Yale University School of Medicine, New Haven, CT, USA
    Neuropsychopharmacology 30:27-34. 2005
    ..It is possible that increased proliferation of these cell types in the frontal cortex could reverse the loss of glial cell number and the reduced volume that has been reported in the frontal cortex of depressed patients...
  69. pmc Aging does not alter the number or phenotype of putative stem/progenitor cells in the neurogenic region of the hippocampus
    Bharathi Hattiangady
    Department of Surgery Neurosurgery, Duke University Medical Center, Durham, NC 27710, United States
    Neurobiol Aging 29:129-47. 2008
    ..Thus, decreased dentate neurogenesis during aging is not attributable to altered number or phenotype of NSCs. Instead, it appears to be an outcome of increased quiescence of NSCs due to changes in NSC milieu...
  70. ncbi Leukemia inhibitory factor is a key signal for injury-induced neurogenesis in the adult mouse olfactory epithelium
    S Bauer
    Unité Mixte de Recherche 5020 Centre National de la Recherche Scientifique, Université Lyon I, 69622 Villeurbanne, France
    J Neurosci 23:1792-803. 2003
    ..Taken together, these results suggest that injured OSNs release LIF as a stimulus to initiate their own replacement...
  71. pmc Cytokinetic analysis of lung cancer by in vivo bromodeoxyuridine labelling
    M M Tinnemans
    Department of Internal Medicine, University Hospital Maastricht, The Netherlands
    Br J Cancer 67:1217-22. 1993
    ..of lung cancer were determined in bronchoscopy specimens after in vivo labelling with the thymidine analogue bromodeoxyuridine (BrdU)...
  72. ncbi Early determination and long-term persistence of adult-generated new neurons in the hippocampus of mice
    Gerd Kempermann
    Max Delbruck Center for Molecular Medicine, Berlin Buch, Robert Rossle Str 10, 13125 Berlin, Germany
    Development 130:391-9. 2003
    ..Dividing cells were labeled with bromodeoxyuridine (BrdU) and were investigated by means of immunofluorescence and confocal microscopy at several time-points 1 ..
  73. ncbi Chronic mild stress impairs cognition in mice: from brain homeostasis to behavior
    Song Li
    Laboratory for Brain and Mind, Institute of Neuroinformatics, Dalian University of Technology, Dalian, 116024, China
    Life Sci 82:934-42. 2008
    ..In addition, severe neuronal cell damage was found, as bromodeoxyuridine (BrdU) positive cells and the expression of brain derived neurotrophic factor (BDNF) in dentate gyrus (DG) ..
  74. pmc Schwann cell-specific ablation of laminin gamma1 causes apoptosis and prevents proliferation
    Wei Ming Yu
    Laboratory of Neurobiology and Genetics, The Rockefeller University, New York, New York 10021, USA
    J Neurosci 25:4463-72. 2005
  75. ncbi Olfactory bulb cells generated in adult male golden hamsters are specifically activated by exposure to estrous females
    Liyue Huang
    Department of Biology, The University of Massachusetts, Amherst 01003, USA
    Horm Behav 41:343-50. 2002
    ..In the first experiment, adult male hamsters were injected with 5'-bromodeoxyuridine (BrdU, 50mg/kg b.w.) 3 times over the course of one week in order to mark dividing cells...
  76. pmc Expression of tryptophan 2,3-dioxygenase in mature granule cells of the adult mouse dentate gyrus
    Koji Ohira
    Division of Systems Medical Science, Institute for Comprehensive Medical Science, Fujita Health University, Toyoake 470 1192, Japan
    Mol Brain 3:26. 2010
    ..These findings indicate that TDO might be required at a late-stage of granule cell development, such as during axonal and dendritic growth, synaptogenesis and its maturation...
  77. ncbi Intrathymically expressed c-kit ligand (stem cell factor) is a major factor driving expansion of very immature thymocytes in vivo
    H R Rodewald
    Basel Institute for Immunology, Switzerland
    Immunity 3:313-9. 1995
    ..or SCF-deficient fetal thymi onto wild-type recipient mice and analyzed the rate of proliferation by in vivo bromodeoxyuridine labeling...
  78. pmc Ganglion cell regeneration following whole-retina destruction in zebrafish
    Tshering Sherpa
    Department of Biological Sciences, University of Idaho, Moscow, ID, USA
    Dev Neurobiol 68:166-81. 2008
    ..This model system for whole-retina regeneration has provided an informative view of the regenerative process...
  79. ncbi Turning astrocytes from the rostral migratory stream into neurons: a role for the olfactory sensory organ
    Mariana Alonso
    Laboratory for Perception and Memory, Centre National de la Recherche Scientifique, Unité de Recherche Associée 2182, Institut Pasteur, 75724 Paris Cedex 15, France
    J Neurosci 28:11089-102. 2008
    ..New neurons in the adult OB can therefore arise from different neurogenic areas that can be separately regulated...
  80. ncbi Adenoviral brain-derived neurotrophic factor induces both neostriatal and olfactory neuronal recruitment from endogenous progenitor cells in the adult forebrain
    A Benraiss
    Department of Neurology and Neuroscience, Cornell University Medical College, New York, New York 10021, USA
    J Neurosci 21:6718-31. 2001
    ..into the lateral ventricles of adult rats, who were treated for 18 d thereafter with the mitotic marker bromodeoxyuridine (BrdU)...
  81. ncbi Light regulates the cell cycle in zebrafish
    Marcus P S Dekens
    Max Planck Institut fur Entwicklungsbiologie, Spemannstrasse 35 39, 72076 Tubingen, Germany
    Curr Biol 13:2051-7. 2003
    ..This represents the first example of a vertebrate cell culture system where circadian rhythms of the cell cycle are observed. Thus, we implicate the cell-autonomous circadian clock in the regulation of the vertebrate cell cycle by light...
  82. ncbi Adult neurogenesis in mammals: an identity crisis
    Pasko Rakic
    Department of Neurobiology, Yale University School of Medicine, New Haven, Connecticut 06520, USA
    J Neurosci 22:614-8. 2002
  83. ncbi Dopamine depletion impairs precursor cell proliferation in Parkinson disease
    Günter U Höglinger
    INSERM U289 Hôpital de la Salpetrière, F 75651 Paris, France
    Nat Neurosci 7:726-35. 2004
    ..These observations suggest that the generation of neural precursor cells is impaired in Parkinson disease as a consequence of dopaminergic denervation...
  84. ncbi The window and mechanisms of major age-related decline in the production of new neurons within the dentate gyrus of the hippocampus
    Muddanna S Rao
    Department of Surgery Division of Neurosurgery, Duke University Medical Center, Durham, NC 27710, USA
    Aging Cell 5:545-58. 2006
    ..subgranular zone (SGZ) of 4-, 12- and 24-month-old rats using four injections (one every sixth hour) of 5'-bromodeoxyuridine (BrdU), and BrdU-DCX dual immunostaining...
  85. ncbi Stress differentially regulates the effects of voluntary exercise on cell proliferation in the dentate gyrus of mice
    Timal S Kannangara
    Division of Medical Sciences, University of Victoria, Victoria, British Columbia, Canada
    Hippocampus 19:889-97. 2009
    ..In this study, we use bromodeoxyuridine and proliferating cell nuclear antigen immunohistochemistry to show that voluntary exercise produces a ..
  86. ncbi Expression of neuron-specific tubulin defines a novel population in the proliferative layers of the developing telencephalon
    J R Menezes
    Department of Anatomy and Cell Biology, Emory University School of Medicine, Atlanta, Georgia 30322
    J Neurosci 14:5399-416. 1994
    ..cells in the cortical-ventricular and subventricular zones, brains were double labeled with TuJ1 and bromodeoxyuridine according to different pulse-chase schedules...
  87. ncbi Repeated estradiol administration alters different aspects of neurogenesis and cell death in the hippocampus of female, but not male, rats
    J M Barker
    Department of Psychology, Graduate Program in Neuroscience, Brain Research Centre, University of British Columbia, 2136 West Mall, Vancouver, British Columbia, Canada
    Neuroscience 152:888-902. 2008
    ..We therefore demonstrate a clear sex difference in the response to estradiol of hippocampal neurogenesis and apoptosis in adult rats, with adult females being more responsive to the effects of estradiol than males...
  88. pmc Estimating dormant and active hematopoietic stem cell kinetics through extensive modeling of bromodeoxyuridine label-retaining cell dynamics
    Richard C van der Wath
    Computer Laboratory, University of Cambridge, Cambridge, United Kingdom
    PLoS ONE 4:e6972. 2009
    ..amongst the various possible causes including stochastic cellular variation) of the observed long term Bromodeoxyuridine (BrdU) retention, is confirmed by the deterministic and stochastic models presented here...
  89. ncbi Nonrenewal of neurons in the cerebral neocortex of adult macaque monkeys
    Daisuke Koketsu
    Department of Integrated Biosciences, University of Tokyo, Kashiwa, Chiba, Japan
    J Neurosci 23:937-42. 2003
    ..Therefore, we have reexamined this issue in two different Macaque species using the thymidine analog bromodeoxyuridine (BrdU) as an indicator of DNA replication during cell division...
  90. pmc The critical role of cyclin D2 in adult neurogenesis
    Anna Kowalczyk
    Department of Molecular and Cellular Neurobiology, Nencki Institute, 02 093 Warsaw, Poland
    J Cell Biol 167:209-13. 2004
    ..Thus, our results reveal the existence of molecular mechanisms discriminating adult versus developmental neurogeneses...
  91. ncbi Nicotine self-administration impairs hippocampal plasticity
    Djoher Nora Abrous
    Institut National de la Santé et de la Recherche Médicale U259 Laboratoire de Psychobiologie des Comportements Adaptatifs, Domaine de Carreire, 33077 Bordeaux, France
    J Neurosci 22:3656-62. 2002
    ..These results raise an important additional concern for the health consequences of nicotine abuse and open new insight on the possible neural mechanisms of tobacco addiction...
  92. ncbi Ciliary neurotrophic factor mediates dopamine D2 receptor-induced CNS neurogenesis in adult mice
    Peng Yang
    Kentucky Spinal Cord Injury Research Center and Department of Neurological Surgery, University of Louisville, Louisville, Kentucky 40292, USA
    J Neurosci 28:2231-41. 2008
    ..Because CNTF is predominantly expressed in the nervous system, this mechanism and the ability to pharmacologically modulate it have implications for Parkinson's disease and cell-replacement therapies for other disorders...
  93. ncbi Adult hippocampal neurogenesis and voluntary running activity: circadian and dose-dependent effects
    Melissa M Holmes
    Department of Psychology, University of British Columbia, Vancouver, British Columbia, Canada
    J Neurosci Res 76:216-22. 2004
    ..Cell proliferation and net neurogenesis were assessed after incorporation of the thymidine analog bromodeoxyuridine (BrdU) and immunohistochemical detection of BrdU and neuronal markers...
  94. ncbi Asymmetric division and cosegregation of template DNA strands in adult muscle satellite cells
    Vasily Shinin
    Stem Cells and Development, Department of Developmental Biology, Pasteur Institute, CNRS URA 2578, 25 rue du Dr Roux, 75724 Paris Cedex 15, France
    Nat Cell Biol 8:677-87. 2006
    ..The cosegregation of 'immortal' template DNA strands and their link with the asymmetry apparatus has important implications for stem cell biology and cancer...
  95. ncbi Neurogenesis in the adult rat piriform cortex
    Anton Pekcec
    Department of Pharmacology, Toxicology, and Pharmacy, University of Veterinary Medicine, Hannover, Germany
    Neuroreport 17:571-4. 2006
    ..Proliferation rates as detected by 5'-bromodeoxyuridine-labeling proved to be low when compared with the major neurogenic brain regions (i.e...
  96. ncbi The effects of social environment on adult neurogenesis in the female prairie vole
    Christie D Fowler
    Department of Psychology and Program of Neuroscience, Florida State University, Tallahassee, FL 32306, USA
    J Neurobiol 51:115-28. 2002
    ..Together, our data indicate that social environment affects neuron proliferation in a stimulus- and site-specific manner in adult female prairie voles...
  97. ncbi Enhanced hippocampal neurogenesis by intraventricular S100B infusion is associated with improved cognitive recovery after traumatic brain injury
    Andrea Kleindienst
    Department of Neurosurgery, Virginia Commonwealth University Medical Center, Richmond, Virginia, USA
    J Neurotrauma 22:645-55. 2005
    ..Cell proliferation was assessed by injecting the mitotic marker bromodeoxyuridine (BrdU) on day 2 postinjury...
  98. ncbi Drug-dependent requirement of hippocampal neurogenesis in a model of depression and of antidepressant reversal
    Alexandre Surget
    U930, INSERM, Universite Francois Rabelais de Tours, Tours, France
    Biol Psychiatry 64:293-301. 2008
    ..We investigated causality between changes in hippocampal neurogenesis and the effects of both chronic stress and chronic ADs...
  99. ncbi Thymidine analogs are transferred from prelabeled donor to host cells in the central nervous system after transplantation: a word of caution
    Terry C Burns
    Stem Cell Institute, University of Minnesota, 420 Delaware Street, Minneapolis, Minnesota 55455, USA
    Stem Cells 24:1121-7. 2006
    Thymidine analogs, including bromodeoxyuridine, chlorodeoxyuridine, iododeoxyuridine, and tritiated thymidine, label dividing cells by incorporating into DNA during S phase of cell division and are widely employed to identify cells ..
  100. ncbi Astroglia induce neurogenesis from adult neural stem cells
    Hongjun Song
    Molecular Neurobiology Laboratory, Howard Hughes Medical Institute at the Salk Institute, 10010 North Torrey Pines Road, La Jolla, California 92037, USA
    Nature 417:39-44. 2002
  101. ncbi Cellular composition of human glial cultures from adult biopsy brain tissue
    Hannah M Gibbons
    Department of Pharmacology and Clinical Pharmacology, The University of Auckland, Auckland, New Zealand
    J Neurosci Methods 166:89-98. 2007
    ..Therefore, although cultures from adult human brain tissue provide an excellent resource for studying human glial cells, careful consideration must be given to their cellular composition when performing studies using these methods...

Research Grants63

  1. Hematopoietic Stem Cells (HSCs) as Juvenile Protective Factors that Alter Aging
    David E Harrison; Fiscal Year: 2011 assessed by a) rates of cell loss after exposure to 5-fluorouricil (5-FU) and b) uptake and dilution of bromodeoxyuridine (BrdU) after 1, 3, and 10 days in drinking water...
  2. Base J and transcription termination in Leishmania
    PETER JOHN MYLER; Fiscal Year: 2013
    ..Furthermore, JPB2 dKO parasites become hypersensitive to growth in bromodeoxyuridine (BrdU), leading to a further reduction in J, even more read-through, and cell death...
  3. Role of beta catenin mediated neural stem cell and astrocyte proliferation in TBI
    Diana M Sama; Fiscal Year: 2013
    ..The proposed studies will greatly enhance my training in the pathobiology of TBI and my technical expertise for studying neurodegenerative conditions. ..
  4. Synaptic Regulation of Neurogenesis in the Dentate Gyrus
    Brian E Derrick; Fiscal Year: 2012
    ..The effects of perforant path stimulation on GC neurogenesis is assessed by administering bromodeoxyuridine (BrdU), a thymidine analog incorporated only in dividing cells, in conjunction with unbiased stereology and ..
  5. Pathogenesis of Myopathy in Models of Myotonic Dystrophy
    Charles Thornton; Fiscal Year: 2009
    ..Third, we will assess myonuclear morphology and bromodeoxyuridine incorporation in HSA(LR) mice to test the hypothesis that accumulation of CUG expansion RNA leads to nuclear ..
  6. Lacrimal Gland Regeneration:Identification and Isolation of Progenitor Cells
    Darlene A Dartt; Fiscal Year: 2010
    ..We will then label proliferating cells from injured mouse lacrimal glands with bromodeoxyuridine to follow cell lineages during regeneration...
  7. Huntington's Disease and Neurogenesis
    Lisa M Ellerby; Fiscal Year: 2012
    ..R6/2 mice with subcutaneous fibroblast growth factor-2 (FGF-2), and found that FGF-2 increased the number of bromodeoxyuridine (BrdU)-labeled, doublecortin (DCX)-expressing cells in the SVZ by ~30% in wild-type mice, and by ~150% in HD ..
  8. Olfactory Bulb Cell Genesis and Survival in a Model of Reversible Deafferentation
    Christine A Byrd; Fiscal Year: 2011
    ..b>Bromodeoxyuridine immunocytochemistry and TUNEL methods will be used to label newly formed cells and apoptotic cells, ..
  9. Characterization of Neural Stem Cells in the Postnatal Mammalian Hypothalamus
    DANIEL ALLEN LEE; Fiscal Year: 2011
    ..We will use bromodeoxyuridine administration, to prospectively determine if proliferating cells within the postnatal HVZ may give rise to ..
    CHERYL SANCHEZ; Fiscal Year: 2003
    ..In the current project, chondrocyte proliferation will be assessed by bromodeoxyuridine (BrdU) incorporation and apoptosis of hypertrophic chondrocytes will be measured by the TUNEL assay in rats ..
  11. Growth Factor-Induced Peripheral Gliogenesis
    Donald Pizzo; Fiscal Year: 2007
    ..Infusion of NGF for 2 weeks results in a massive increase in Schwann cells detected by using bromodeoxyuridine (BrdU) to label dividing cells...
    Clement Ip; Fiscal Year: 2003
    ..These biomarkers include (a) bromodeoxyuridine labeling for DNA synthesis, (b) cyclins D1, E and A, and (c) cdk2, cdk4 and cdk6...
  13. Hormonal Regulation of the Cervix During Pregnancy
    ORRIN SHERWOOD; Fiscal Year: 2006
    ..Treatment with RU 486 alone and in combination with exogenous relaxin will be done at term with relaxin-deficient rats. ..
  14. Jun Kinase Signaling and Apoptosis in Ischemia Stroke
    Chia Yi Kuan; Fiscal Year: 2006
    ..the apoptotic neurons in cerebral ischemia-hypoxia progress into the S-phase of cell cycle and incorporate bromodeoxyuridine (BrdU). These results suggest: (1)...
    John Baron; Fiscal Year: 1992
    ..biopsy of the rectal mucosa, with subsequent assessment of labelling indices (and related measures) based on bromodeoxyuridine and proliferating cell nuclear antigen...
    Robert Schlegel; Fiscal Year: 1993
    ..A monoclonal antibody to bromodeoxyuridine-substituted DNA will be used to visualize microscopically the replication state of DNA that surrounds ..
    PHILIP STETSON; Fiscal Year: 1992
    Richard Davis; Fiscal Year: 1993
    ..The development of monoclonal antibodies (MAb) against bromodeoxyuridine (BUdR or BrdUrd), thymidine analogues, was a breakthrough for cell kinetic studies on human tumors in situ, ..
    Jonathan Maybaum; Fiscal Year: 1990
    ..In addition to TG, substitutions will be performed using ara-C, ara-A or bromodeoxyuridine. 2. Testing the biological effects of sequence-specific substitution...
  20. Novel Tools for Research on Primate Spermatogonia
    Stefan Schlatt; Fiscal Year: 2005
    ..The dual fluorescence labeling of bromodeoxyuridine and acrosin in conjunction with confocal microscopy allows the description of the clonogenic and spatial ..
    Jonathan Kurie; Fiscal Year: 1999 investigated by measurements of S phase fraction (flow cytometry and incorporation of 3H-thymidine and bromodeoxyuridine)...
    Mou Tuan Huang; Fiscal Year: 1999 intact female Sencar mice inhibits proliferation of the mammary gland and uterus as determined by the bromodeoxyuridine (BrdUr) labeling index and uterine wet weight during the estrous phase of the estrous cycle...
    Michael Wargovich; Fiscal Year: 1993
    ..During the experiment intermediate markers of colonic proliferation, including tritiated thymidine uptake, bromodeoxyuridine immunoperoxidase labeling, ornithine decarboxylase activity and protein kinase C activity, will be measured ..
    Nobuyuki Ito; Fiscal Year: 1990
    ..compartments of the liver, attention will be concentrated on cell proliferation as assessed by bromodeoxyuridine incorporation and enzyme phenotype in all of the possible target tissues...
    R White; Fiscal Year: 1993
    ..Tumor cells synthesizing DNA are labeled using a halogenated thymidine analogue, either bromodeoxyuridine or iododeoxyuridine, and sampled several hours later...
    Caroline Damsky; Fiscal Year: 1992
    ..adhesion variants will be used whose expression of integrins can be modulated over a 20 fold range by 5-bromodeoxyuridine. Specific antibodies that recognize individual subunits of the regulated integrin dimeric complexes will be ..
    Barry Rosenstein; Fiscal Year: 1993
    ..First, the overall level of excision repair will be measured in solar UV-irradiated cells using the modified bromodeoxyuridine photolysis assay that was developed as part of this project...
    Zhiwei Zhang; Fiscal Year: 2003
    ..kidney is also co-localized with the de-differentiation marker vimentin and proliferation marker bromodeoxyuridine. In order to study the functions of KIM-1, this laboratory has generated a fusion protein containing the ..
    Kevin Osteen; Fiscal Year: 1992
    ..epithelial and stromal cells will be examined using established methodology to monitor cell proliferation (bromodeoxyuridine uptake) and differentiation (expression of stage-specific marker epitopes)...
    REGINA ARMSTRONG; Fiscal Year: 1999
    ..mice during early remyelination, and mitogenic effects of exogenous growth factors will be tested using a bromodeoxyuridine incorporation assay...
    S Rajkumar; Fiscal Year: 2001
    ..cell apoptosis; plasma cells in S-phase and circulating plasma cells will be estimated using slide based bromodeoxyuridine immunofluorescent assays...
    JAMIL SCOTT; Fiscal Year: 2006
    ..from the metanephric kidney will be identified and isolated with a nucleotide analog (tritiated thymidine or bromodeoxyuridine)...
    Dominic Poccia; Fiscal Year: 1993
    ..The earliest cells of the lineage will be pulse-labelled with bromodeoxyuridine. Their differentiation will be revealed by the appearance of BrdU in successively more differentiated ..
  34. Lateral Flow Assay for Oncogenic Strains of HPV
    LEONARD BAZAR; Fiscal Year: 2003
    ..and various haptens such as dinitrophenyl (DNP), digoxigenin (DIG), fluorescein isothiocyanate (FITC), or bromodeoxyuridine (BrdU)...
    Dale Hoyt; Fiscal Year: 1999
    ..3 DNA breakage will be measured by in situ end labeling of DNA breaks, and repair will be determined from bromodeoxyuridine incorporation and by the ability of nuclear extracts to repair oligonucleotide substrates...
    CORAL LAMARTINIERE; Fiscal Year: 2000 use from studies on gland differentiation, using mammary whole mounts, and cell proliferation, using bromodeoxyuridine immunohistochemistry (IHC)...
    BARRY RITTMAN; Fiscal Year: 1993
    ..At varying periods of time from preparation of implants or chimeras, mice will be injected with 5-bromodeoxyuridine (BrdU), sacrificed and epithelial cell suspensions prepared...
  38. Adult Neurogenesis, Amygdala, and Social Attachment
    Zuoxin Wang; Fiscal Year: 2003
    ..abstract_text> ..
  39. Use of RNAi to investigate cullin-5 function in breast cancer
    Michael Fay; Fiscal Year: 2006
    ..will be tested by performing cell counts, WST-1 proliferation assays and by measuring the incorporation of bromodeoxyuridine into DNA in Cul5 knock-downs versus cells transfected with a negative control siRNA...
  40. Effects of Sleep Fragmentation on Adult Neurogenesis
    DENNIS J MCGINTY; Fiscal Year: 2010
    ..Cell proliferation will be studied by the bromodeoxyuridine (BrdU) method. Gene expression in hippocampus is assessed by RT-PCR...
    Charles Turner; Fiscal Year: 1999
    ..Bone histomorphometry will be used to measure bone formation rates; immunohistochemistry of bromodeoxyuridine to measure cell division; and serum biochemistry to measure blood chemistry and PTH levels...
    Pradeep Bhide; Fiscal Year: 1999
    ..technique to be used in the present experiments will be labeling cells in -phase of the mitotic cycle with bromodeoxyuridine and/or tritiated thymidine and identifying the labeled cells in tissue sections from the ganglionic eminence ..
    Stephen Schwartz; Fiscal Year: 1980
    Organ cultures of embryonic rat tooth germs will be treated with the thymidine analog 5-bromodeoxyuridine (BrdU) in order to characterize the molecular mechanism(s) responsible for productive epithelial-mesenchymal interactions...
    Stuart Zimmerman; Fiscal Year: 1992
    ..tumor; 2) determination of the kinetic behavior of cell populations labelled with monoclonal antibodies to bromodeoxyuridine and followed through time by flow cytometry in both in vitro and in vivo systems. III...
    PETER CONTI; Fiscal Year: 1999
    ..biochemical radioassay, and autoradiography with [3H] or [14C] TdR, as well as immunohistochemistry with bromodeoxyuridine (BUdR), in order to validate the use of [11C] TdR with PET...
    Timothy Kinsella; Fiscal Year: 2003
    ..MMR-deficient cells show "damage tolerance" following exposures to the halogenated thymidine (dThd) analogs, bromodeoxyuridine (BrdUrd) or iododeoxyuridine (IdUrd); resulting in greater than 2-3 fold higher levels of incorporated drug ..
    LUISA SCOTT; Fiscal Year: 2003
    ..Third, adult-generated neurons will be labelled with bromodeoxyuridine and in situ hybridization will be used to determine if the expression of NMDA receptor mRNAs in these ..
    Lucy Brown; Fiscal Year: 2006
    ..and adult cell proliferation and neurogenesis in mutants and their controls by using a thymidine analogue, bromodeoxyuridine (BrdU)...
  49. Effects of Physical Activity on Hippocampal Neurogenesis
    ALEXIS STRANAHAN; Fiscal Year: 2007
    ..The thymidine analog 5-bromodeoxyuridine (BrdU) is typically used in studies of cell proliferation and survival in the hippocampus...
    Jonathan Sprent; Fiscal Year: 2004
    ..Labeling studies with DNA precursors such as bromodeoxyuridine (BrdU) have shown that the turnover of memory-phenotype T-cells in vitro is far higher than for naive-..
  51. Function-based selection of target genes in tumor cells
    IGOR RONINSON; Fiscal Year: 2005
    ..Such GSEs can be isolated by bromodeoxyuridine (BrdU) suicide selection from a normalized (reduced-redundance) library of human cDNA fragments in an ..
    HOWARD GRATZNER; Fiscal Year: 1993
    ..the effects of chemotherapeutic drugs on cell proliferation by means of the immunofluorescent detection of bromodeoxyuridine as a measure of DNA synthesis...
    Andrew Chan; Fiscal Year: 2007
    ..These are achieved by using the bromodeoxyuridine incorporation assay to quantify mitogenesis, immunological staining with a panel of neuronal specific ..
    Erwin Goldberg; Fiscal Year: 1992
    ..Recombinants (tk-) grown in human 143B cells (tk-) will be selected by resistance to 5' bromodeoxyuridine. Each of the peptides and viral vectors produced will be tested for its ability to evoke antibodies in an ..
  55. Marijuana & Cytokine Actions on Rat Brain Mitogenesis
    Barry Jacobs; Fiscal Year: 2004
    ..Following these various drug treatments (or control treatments), all animals will be administered bromodeoxyuridine (BrdU) and sacrificed two hours later...
    Maurice Barcos; Fiscal Year: 1992
    ..of cells in the S-phase of the proliferative cycle (S%), as determined by flow cytometry or 3H-thymidine, bromodeoxyuridine (BUdR) and Ki-67 labelling indices, provide independent prognostic information in patients with breast ..
  57. Augmentation of Neurogenesis and Recovery After Stroke
    Jack Parent; Fiscal Year: 2007
    ..Progress in these aims will advance our understanding of mechanisms underlying neural stem cell regulation and rehabilitative plasticity after brain injury, and may lead to novel regenerative therapies for stroke. ..
  58. Adult Forebrain Neural Stem Cell Response to Ischemia
    Jack Parent; Fiscal Year: 2005
    ..Progress in these Aims will advance our understanding of adult SVZ neurogenesis in the normal and ischemic brain, and may provide insight into the potential use of neural stem cells for stroke therapy. ..
    LAURA NGWENYA; Fiscal Year: 2005
    ..b>Bromodeoxyuridine (BrdU, a marker of cell proliferation) will be injected into both young and old rhesus monkeys (Macaca ..
  60. Combination Therapy for Chronic Stroke-Induced Impairment
    J Leigh Leasure; Fiscal Year: 2008
    ..The proposed experiment will test a potential treatment for chronic stroke-related impairment in the aged brain. [unreadable] [unreadable] [unreadable]..
  61. Modulation of Hippocampal Neurogenesis and Cognitive Recovery Following Trauma
    Dong Sun; Fiscal Year: 2010
  62. Development of plasmacytoid dendritic cells
    David Allman; Fiscal Year: 2008
    ..Determine the actual contribution of Rag-2+ lymphoid progenitors to each peripheral DC subset including PDCs; and 3. Identify cytokines that can redirect early B cell progenitors into the DC lineage in vitro and in vivo. ..
  63. Hormones, Experience and Hippocampal Neurogenesis
    Elizabeth Gould; Fiscal Year: 2009
    ..The production, survival and phenotype of new cells in the hippocampus will be assessed with bromodeoxyuridine (BrdU) labeling combined with immunocytochemistry for cell-type specific markers, hormone levels will be ..