deoxyadenosines

Summary

Summary: Adenosine molecules which can be substituted in any position, but are lacking one hydroxyl group in the ribose part of the molecule.

Top Publications

  1. ncbi Cordyceps: a traditional Chinese medicine and another fungal therapeutic biofactory?
    R Russell M Paterson
    Institute for Biotechnology and Bioengineering IBB, Centre of Biological Engineering, Campus de Gualtar, University of Minho, Braga, Portugal
    Phytochemistry 69:1469-95. 2008
  2. pmc Structure and stability of duplex DNA containing (5'S)-5',8-cyclo-2'-deoxyadenosine: an oxidatively generated lesion repaired by NER
    Tatiana Zaliznyak
    Department of Pharmacological Sciences, School of Medicine, Stony Brook University, Stony Brook, New York 11794 8651, USA
    Chem Res Toxicol 25:2103-11. 2012
  3. ncbi Antitumor effect of cordycepin (3'-deoxyadenosine) on mouse melanoma and lung carcinoma cells involves adenosine A3 receptor stimulation
    Kazuki Nakamura
    Department of Pharmacology, Faculty of Pharmaceutical Sciences, Mukogawa Women s University, Nishinomiya, Hyogo 663 8179, Japan
    Anticancer Res 26:43-7. 2006
  4. ncbi c-Jun N-terminal kinase 1 is required for cordycepin-mediated induction of G2/M cell-cycle arrest via p21WAF1 expression in human colon cancer cells
    Se Jung Lee
    Department of Food and Biotechnology, Chungju National University, Chungju, Chungbuk 380 702, South Korea
    Food Chem Toxicol 48:277-83. 2010
  5. ncbi Inhibitory effect of cordycepin on hematogenic metastasis of B16-F1 mouse melanoma cells accelerated by adenosine-5'-diphosphate
    Noriko Yoshikawa
    Department of Pharmacology, Faculty of Pharmaceutical Sciences, Mukogawa Women s University, 11 68, Koshien Kyuban cho, Nishinomiya, Hyogo 663 8179, Japan
    Anticancer Res 29:3857-60. 2009
  6. ncbi 2'-deoxy-4'-C-ethynyl-2-fluoroadenosine: a nucleoside reverse transcriptase inhibitor with highly potent activity against wide spectrum of HIV-1 strains, favorable toxic profiles, and stability in plasma
    Hiroshi Ohrui
    Yokomama College of Pharmacy, 601 Matano cho, Totsuka, Yokohama, Japan
    Nucleosides Nucleotides Nucleic Acids 26:1543-6. 2007
  7. pmc Complete release of (5'S)-8,5'-cyclo-2'-deoxyadenosine from dinucleotides, oligodeoxynucleotides and DNA, and direct comparison of its levels in cellular DNA with other oxidatively induced DNA lesions
    Pawel Jaruga
    Department of Chemical and Biochemical Engineering, University of Maryland Baltimore County, MD 22777, USA
    Nucleic Acids Res 32:e87. 2004
  8. pmc Quantification of oxidative DNA lesions in tissues of Long-Evans Cinnamon rats by capillary high-performance liquid chromatography-tandem mass spectrometry coupled with stable isotope-dilution method
    Jin Wang
    Department of Chemistry, University of California, Riverside, California 92521 0403, United States
    Anal Chem 83:2201-9. 2011
  9. ncbi DNA damage products (5'R)- and (5'S)-8,5'-cyclo-2'-deoxyadenosines as potential biomarkers in human urine for atherosclerosis
    Pawel Jaruga
    Biochemical Science Division, National Institute of Standards and Technology, Gaithersburg, Maryland 20899, United States
    Biochemistry 51:1822-4. 2012
  10. ncbi Cordycepin stimulated steroidogenesis in MA-10 mouse Leydig tumor cells through the protein kinase C Pathway
    Hsiang Yin Pao
    Department of Cell Biology and Anatomy, College of Medicine, National Cheng Kung University, Tainan 70101, Taiwan, Republic of China
    J Agric Food Chem 60:4905-13. 2012

Detail Information

Publications226 found, 100 shown here

  1. ncbi Cordyceps: a traditional Chinese medicine and another fungal therapeutic biofactory?
    R Russell M Paterson
    Institute for Biotechnology and Bioengineering IBB, Centre of Biological Engineering, Campus de Gualtar, University of Minho, Braga, Portugal
    Phytochemistry 69:1469-95. 2008
    ..Polysaccharides and secondary metabolites are of particular interest. The use of genuine anamorphic forms in bioreactors is encouraged...
  2. pmc Structure and stability of duplex DNA containing (5'S)-5',8-cyclo-2'-deoxyadenosine: an oxidatively generated lesion repaired by NER
    Tatiana Zaliznyak
    Department of Pharmacological Sciences, School of Medicine, Stony Brook University, Stony Brook, New York 11794 8651, USA
    Chem Res Toxicol 25:2103-11. 2012
    ..The S-cdA·T duplex structure shows many similarities to and some intriguing differences from the recently reported structure of an S-cdG·dC duplex³¹ that suggest different lesion site dynamics...
  3. ncbi Antitumor effect of cordycepin (3'-deoxyadenosine) on mouse melanoma and lung carcinoma cells involves adenosine A3 receptor stimulation
    Kazuki Nakamura
    Department of Pharmacology, Faculty of Pharmaceutical Sciences, Mukogawa Women s University, Nishinomiya, Hyogo 663 8179, Japan
    Anticancer Res 26:43-7. 2006
    ..These results suggest that cordycepin exerts inhibitory effects on the growth of mouse melanoma and lung carcinoma cells by stimulating adenosine A3 receptors on tumor cells...
  4. ncbi c-Jun N-terminal kinase 1 is required for cordycepin-mediated induction of G2/M cell-cycle arrest via p21WAF1 expression in human colon cancer cells
    Se Jung Lee
    Department of Food and Biotechnology, Chungju National University, Chungju, Chungbuk 380 702, South Korea
    Food Chem Toxicol 48:277-83. 2010
    ..Together, these results suggest a critical role for JNK1 activation in cordycepin-induced inhibition of cell growth and G2/M-phase arrest in human colon cancer cells...
  5. ncbi Inhibitory effect of cordycepin on hematogenic metastasis of B16-F1 mouse melanoma cells accelerated by adenosine-5'-diphosphate
    Noriko Yoshikawa
    Department of Pharmacology, Faculty of Pharmaceutical Sciences, Mukogawa Women s University, 11 68, Koshien Kyuban cho, Nishinomiya, Hyogo 663 8179, Japan
    Anticancer Res 29:3857-60. 2009
    ..These results suggest that ADP accelerated hematogenic metastasis and cordycepin has an inhibitory effect on hematogenic metastasis of B16-F1 melanoma cells via blocking of ADP-induced platelet aggregation in vivo...
  6. ncbi 2'-deoxy-4'-C-ethynyl-2-fluoroadenosine: a nucleoside reverse transcriptase inhibitor with highly potent activity against wide spectrum of HIV-1 strains, favorable toxic profiles, and stability in plasma
    Hiroshi Ohrui
    Yokomama College of Pharmacy, 601 Matano cho, Totsuka, Yokohama, Japan
    Nucleosides Nucleotides Nucleic Acids 26:1543-6. 2007
    ..The nucleoside will prevent or delay the emergence of drug-resistant HIV-1 variants and be an ideal therapeutic agent for both HIV-1 and HBV infections...
  7. pmc Complete release of (5'S)-8,5'-cyclo-2'-deoxyadenosine from dinucleotides, oligodeoxynucleotides and DNA, and direct comparison of its levels in cellular DNA with other oxidatively induced DNA lesions
    Pawel Jaruga
    Department of Chemical and Biochemical Engineering, University of Maryland Baltimore County, MD 22777, USA
    Nucleic Acids Res 32:e87. 2004
    ..We conclude that (5'S)-cdA can be completely released from DNA by enzymic hydrolysis, and the level of (5'S)-cdA in tissue DNA is comparable to those of other oxidatively induced DNA lesions...
  8. pmc Quantification of oxidative DNA lesions in tissues of Long-Evans Cinnamon rats by capillary high-performance liquid chromatography-tandem mass spectrometry coupled with stable isotope-dilution method
    Jin Wang
    Department of Chemistry, University of California, Riverside, California 92521 0403, United States
    Anal Chem 83:2201-9. 2011
    ....
  9. ncbi DNA damage products (5'R)- and (5'S)-8,5'-cyclo-2'-deoxyadenosines as potential biomarkers in human urine for atherosclerosis
    Pawel Jaruga
    Biochemical Science Division, National Institute of Standards and Technology, Gaithersburg, Maryland 20899, United States
    Biochemistry 51:1822-4. 2012
    ..Our data suggest that R-cdA and S-cdA can be accurately and reproducibly measured in human urine as potential biomarkers of risk and diagnosis for atherosclerosis...
  10. ncbi Cordycepin stimulated steroidogenesis in MA-10 mouse Leydig tumor cells through the protein kinase C Pathway
    Hsiang Yin Pao
    Department of Cell Biology and Anatomy, College of Medicine, National Cheng Kung University, Tainan 70101, Taiwan, Republic of China
    J Agric Food Chem 60:4905-13. 2012
    ..In conclusion, cordycepin stimulated intracellular PLC/PKC and MAPK signal transduction pathways to induce steroidogenesis and cell death in MA-10 mouse Leydig tumor cells...
  11. pmc Response of simian immunodeficiency virus to the novel nucleoside reverse transcriptase inhibitor 4'-ethynyl-2-fluoro-2'-deoxyadenosine in vitro and in vivo
    Michael Murphey-Corb
    Department of Microbiology and Molecular Genetics, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA
    Antimicrob Agents Chemother 56:4707-12. 2012
    ..These results suggest that expanded studies with EFdA are warranted...
  12. ncbi 2'-deoxy-4'-C-ethynyl-2-halo-adenosines active against drug-resistant human immunodeficiency virus type 1 variants
    Atsushi Kawamoto
    Laboratory of Virus Immunology, Institute for Virus Research, Kyoto University, 53 Kawaramachi, Shogoin, Sakyo ku, Kyoto 606 8507, Japan
    Int J Biochem Cell Biol 40:2410-20. 2008
    ..The present data suggest that EFdA, is a promising candidate for developing as a therapeutic agent for the treatment of individuals harboring multi-drug resistant HIV variants...
  13. ncbi Cordycepin (3'-deoxyadenosine) inhibits the growth of B16-BL6 mouse melanoma cells through the stimulation of adenosine A3 receptor followed by glycogen synthase kinase-3beta activation and cyclin D1 suppression
    Noriko Yoshikawa
    Department of Pharmacology, School of Pharmacy and Pharmaceutical Sciences, Mukogawa Women s University, 11 68, Koshien Kyuban cho, Nishinomiya, Hyogo, 663 8179, Japan
    Naunyn Schmiedebergs Arch Pharmacol 377:591-5. 2008
    ..In conclusion, this study demonstrated that cordycepin inhibits the proliferation of B16-BL6 cells by stimulating adenosine A3 receptors followed by the Wnt signaling pathway, including GSK-3beta activation and cyclin D1 inhibition...
  14. ncbi High urinary excretion of lipid peroxidation-derived DNA damage in patients with cancer-prone liver diseases
    Jagadeesan Nair
    Division of Toxicology and Cancer Risk Factors, German Cancer Research Center DKFZ, Heidelberg, Germany
    Mutat Res 683:23-8. 2010
    ....
  15. pmc Catalytic activity of the anaerobic tyrosine lyase required for thiamine biosynthesis in Escherichia coli
    Martin R Challand
    School of Chemistry, University of Southampton, Highfield, Southampton SO17 1BJ, United Kingdom
    J Biol Chem 285:5240-8. 2010
    ..The results of the substrate analogue studies and the product inhibition can be explained by a mechanistic hypothesis involving two reaction pathways, a product-forming pathway and a futile cycle...
  16. ncbi The photochemistry of 8-bromo-2'-deoxyadenosine. A direct entry to cyclopurine lesions
    Liliana B Jimenez
    Departamento de Quimica Instituto de Tecnologia Quimica UPV CSIC, Universidad Politecnica de Valencia, Camino de Vera s n, E 46022, Valencia, Spain
    Photochem Photobiol Sci 3:1042-6. 2004
    ....
  17. ncbi Measurement of (5'R)- and (5'S)-8,5'-cyclo-2'-deoxyadenosines in DNA in vivo by liquid chromatography/isotope-dilution tandem mass spectrometry
    Pawel Jaruga
    Chemical Science and Technology Laboratory, National Institute of Standards and Technology, Gaithersburg, MD 20899, USA
    Biochem Biophys Res Commun 386:656-60. 2009
    Oxidatively induced DNA lesions (5'R)- and (5'S)-8,5'-cyclo-2'-deoxyadenosines (R-cdA and S-cdA) are detectable and accumulate in vivo due to disease states and defects in DNA repair...
  18. ncbi 5',8-Cyclo-2'-deoxyadenosine (cdA) formation by gamma-radiation. Theoretical quantum mechanics study
    Boleslaw T Karwowski
    Laboratoire des Lésions des Acides Nucléiques, INAC SCIB UMR E n degrees 3 CEA UJF, CEA Grenoble, Grenoble Cedex 9, France
    Acta Biochim Pol 56:655-62. 2009
    ..The O4'-exo conformation was found to be present in each of the discussed mechanisms...
  19. ncbi Proteomic detection of changes in protein expression induced by cordycepin in human hepatocellular carcinoma BEL-7402 cells
    Ping Shi
    Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, People s Republic of China
    Methods Find Exp Clin Pharmacol 30:347-53. 2008
    ..It is suggested that the effect of cordycepin on the growth of tumor cells is significantly related to the metabolism-associated protein expression induced by cordycepin...
  20. ncbi Naphthalene-based environmentally sensitive fluorescent 8-substituted 2'-deoxyadenosines: application to DNA detection
    Azusa Suzuki
    Department of Chemical Biology and Applied Chemistry, School of Engineering, Nihon University, Koriyama, Fukushima 963 8642, Japan
    Bioorg Med Chem Lett 23:886-92. 2013
    ..We developed a new fluorescent probe containing (2,6dn)A that can be used for the detection of target DNA via a bulge formation regardless of the neighboring sequences...
  21. ncbi Cordycepin inhibits vascular smooth muscle cell proliferation
    Woochul Chang
    Cardiovascular Research Institute, Cardiology Division, Department of Internal Medicine, Brain Korea 21 Project for Medical Science, Yonsei University College of Medicine, Seoul, 120 752, South Korea
    Eur J Pharmacol 597:64-9. 2008
    ..Taken together, these data suggest that cordycepin may induce antiproliferation in RAoSMCs via the modulation of vessel wall remodeling. Therefore, cordycepin may be a potential therapeutic approach to treat restenosis...
  22. pmc HO* radicals induce an unexpected high proportion of tandem base lesions refractory to repair by DNA glycosylases
    Francois Bergeron
    Commissariat a l Energie Atomique, Institut Nanosciences et Cryogenie, Lésions des Acides Nucléiques, F 38054 Grenoble Cedex 9, France
    Proc Natl Acad Sci U S A 107:5528-33. 2010
    ....
  23. ncbi RNA-directed agent, cordycepin, induces cell death in multiple myeloma cells
    Lisa S Chen
    Department of Experimental Therapeutics, University of Texas M D Anderson Cancer Center, Houston, TX 77030, USA
    Br J Haematol 140:682-391. 2008
    ..Our results suggest that RNA-directed agents may be a new group of agents for the treatment of MM...
  24. pmc Repair efficiency of (5'S)-8,5'-cyclo-2'-deoxyguanosine and (5'S)-8,5'-cyclo-2'-deoxyadenosine depends on the complementary base
    Paritosh Pande
    Department of Chemistry, University of Connecticut, Storrs, CT 06269, USA
    DNA Repair (Amst) 11:926-31. 2012
    ..This result adds to complexity of human NER, which discriminates the damaged base pairs on the basis of multiple criteria...
  25. ncbi The nucleoside antagonist cordycepin causes DNA double strand breaks in breast cancer cells
    Hong Jue Lee
    Department of Obstetrics and Gynecology, Ludwig Maximilians University Munich, Campus Grosshadern, Munich, Germany
    Invest New Drugs 30:1917-25. 2012
    ....
  26. pmc Blockade of adipocyte differentiation by cordycepin
    Shuhei Takahashi
    Department of Molecular Signaling, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, Chuo, Yamanashi, Japan
    Br J Pharmacol 167:561-75. 2012
    ..However, the active entities in the extract and the underlying mechanisms of its action are not known. Hence, we investigated whether and how cordycepin (3'-deoxyadenosine), a constituent of C. militaris, inhibits adipogenesis...
  27. pmc Structures of (5'S)-8,5'-Cyclo-2'-deoxyguanosine Mismatched with dA or dT
    Hai Huang
    Department of Chemistry, Center in Molecular Toxicology, and Center for Structural Biology, Vanderbilt Ingram Cancer Center, Vanderbilt University, Nashville, Tennessee 37235, United States
    Chem Res Toxicol 25:478-90. 2012
    ..For the S-cdG·dA mismatch pair, both S-cdG and dA intercalate, but no hydrogen bonding is observed between S-cdG and dA. This is consistent with the lower levels of S-cdG → T transitions in E. coli...
  28. ncbi Beneficial effects of cordycepin on metabolic profiles of liver and plasma from hyperlipidemic hamsters
    Yang Sun
    Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China
    J Asian Nat Prod Res 13:534-46. 2011
    ..Cordycepin afforded a little lipid-regulating activity on plasma but more beneficial effects on liver, implicating that cordycepin might have a protective effect on liver under fatty liver condition...
  29. ncbi Induction of apoptosis by cordycepin via reactive oxygen species generation in human leukemia cells
    Jin Woo Jeong
    Department of Biochemistry and Research Institute of Oriental Medicine, Dongeui University College of Oriental Medicine, Busan 614 052, Republic of Korea
    Toxicol In Vitro 25:817-24. 2011
    ..These results support a mechanism whereby cordycepin induces apoptosis of human leukemia cells through a signaling cascade involving a ROS-mediated caspase pathway...
  30. ncbi Biotin synthase exhibits burst kinetics and multiple turnovers in the absence of inhibition by products and product-related biomolecules
    Christine E Farrar
    Department of Chemistry, University of Hawaii at Manoa, Honolulu, Hawaii 96822, United States
    Biochemistry 49:9985-96. 2010
    ....
  31. ncbi Cordycepin induces apoptosis of CGTH W-2 thyroid carcinoma cells through the calcium-calpain-caspase 7-PARP pathway
    Ying Chen
    Department of Anatomy and Cell Biology, College of Medicine, National Taiwan University, Taipei 100, Taiwan
    J Agric Food Chem 58:11645-52. 2010
    ..In conclusion, the cordycepin-induced apoptosis of CGTH W-2 cells is mediated by the calcium-calpain-caspase 7-PARP pathway, and ARs are involved in the apoptotic effect of cordycepin...
  32. ncbi Divergent effects of oxidatively induced modification to the C8 of 2'-deoxyadenosine on transcription factor binding: 8,5'(S)-cyclo-2'-deoxyadenosine inhibits the binding of multiple sequence specific transcription factors, while 8-oxo-2'-deoxyadenosine i
    Jessy Abraham
    Section on Molecular Neurobiology, Laboratory of Neurogenetics, National Institute on Alcohol Abuse and Alcoholism, Rockville, Maryland 20852, USA
    Environ Mol Mutagen 52:287-95. 2011
    ..The cyclo-dAdo lesion, in contrast, distorted the DNA structure, providing an explanation for the inhibition of NF-kappaB binding...
  33. ncbi Cordycepin suppresses TNF-alpha-induced invasion, migration and matrix metalloproteinase-9 expression in human bladder cancer cells
    Eo Jin Lee
    Department of Food and Biotechnology, Chungju National University, Chungju, Chungbuk 380 702, South Korea
    Phytother Res 24:1755-61. 2010
    ..These results suggest that cordycepin maybe an effective therapeutic approach to treat bladder cancer...
  34. ncbi Effect of (5'S)-5',8-cyclo-2'-deoxyadenosine on the conformation of di and trinucleotides. A NMR and DFT study
    Boleslaw T Karwowski
    Laboratoire des Lésions des Acides Nucléiques, INAC SCIB UMR E n degrees 3 CEA UJF, Condensée, CEA Grenoble, 17 Avenue des Martyrs, F 38054, Grenoble Cedex 9, France
    Org Biomol Chem 6:3408-13. 2008
    ....
  35. ncbi 8,5'-Cyclopurine-2'-deoxynucleosides in DNA: mechanisms of formation, measurement, repair and biological effects
    Pawel Jaruga
    Chemical Science and Technology Laboratory, National Institute of Standards and Technology, Gaithersburg, MD 20899, USA
    DNA Repair (Amst) 7:1413-25. 2008
    ..Accumulation of cdA and cdG was observed in tissues in vivo in connection to disease and environmental conditions, suggesting an important role for these lesions in disease processes including carcinogenesis and neuronal death...
  36. ncbi The fate of C5' radicals of purine nucleosides under oxidative conditions
    Fabien Boussicault
    ISOF, Consiglio Nazionale delle Ricerche, Via P Gobetti 101, 40129 Bologna, Italy
    J Am Chem Soc 130:8377-85. 2008
    ..Rate constants for the reactions of the 2'-deoxyadenosin-5'-yl with cysteine and glutathione in water were determined by pulse radiolysis to be (2.1 +/- 0.5) x 10(7) and (4.9 +/- 0.6) x 10(7) M(-1) s(-1) at 22 degrees C, respectively...
  37. ncbi Formation of 5',8-cyclo-2'-deoxyadenosine in single strand DNA. Theoretical quantum mechanics study
    Boleslaw T Karwowski
    Department of Biopharmacy Medical University of Lodz, Muszynskiego Street 1, 90 151, Lodz, Poland
    Org Biomol Chem 8:1603-9. 2010
    ..Moreover, the investigated reaction paths have shown that the formation of the 5'R isomer is energetically favourable in both the aqueous and gaseous phases. Therefore, the presented results are in good agreement with experimental data...
  38. ncbi Identification and quantification of 8,5'-cyclo-2'-deoxy-adenosine in DNA by liquid chromatography/ mass spectrometry
    M Dizdaroglu
    Chemical Science and Technology Laboratory, National Institute of Standards and Technology, Gaithersburg, MD 20899 8311, USA
    Free Radic Biol Med 30:774-84. 2001
    ..The measurement of 8,5'-cyclo-2'-deoxyadenosine by liquid chromatography/mass spectrometry may contribute to the understanding of its biological properties and its role in diseases with defective nucleotide-excision repair...
  39. ncbi Model studies of DNA C5' radicals. Selective generation and reactivity of 2'-deoxyadenosin-5'-yl radical
    Chryssostomos Chatgilialoglu
    ISOF, Consiglio Nazionale delle Ricerche, Via P Gobetti 101, 40129 Bologna, Italy
    J Am Chem Soc 125:3839-48. 2003
    ....
  40. pmc Roles of RubisCO and the RubisCO-like protein in 5-methylthioadenosine metabolism in the Nonsulfur purple bacterium Rhodospirillum rubrum
    Jaya Singh
    Department of Microbiology, The Ohio State University, 484 West 12th Avenue, Columbus, OH 43210 1292, USA
    J Bacteriol 192:1324-31. 2010
    ..Moreover, the aerobic and anaerobic sulfur salvage pathways appear to be differentially controlled, with novel and previously undescribed steps apparent for sulfur salvage in this organism...
  41. ncbi C5'-adenosinyl radical cyclization. A stereochemical investigation
    Maria Luisa Navacchia
    ISOF, Consiglio Nazionale delle Ricerche, Via P Godetti 101, 40129 Bologna, Italy
    J Org Chem 71:4445-52. 2006
    ....
  42. ncbi Identification and quantification of (5'R)- and (5'S)-8,5'-cyclo-2'-deoxyadenosines in human urine as putative biomarkers of oxidatively induced damage to DNA
    Pawel Jaruga
    Biochemical Science Division, National Institute of Standards and Technology, Gaithersburg, MD 20899, USA
    Biochem Biophys Res Commun 397:48-52. 2010
    ..This study shows, for the first time, that R-cdA and S-cdA exist in human urine and can be identified and quantified by LC-MS/MS. We propose that R-cdA and S-cdA may be well-suited biomarkers for disease processes such as carcinogenesis...
  43. ncbi Lipid peroxidation-induced DNA damage in cancer-prone inflammatory diseases: a review of published adduct types and levels in humans
    Urmila Nair
    Division of Toxicology and Cancer Risk Factors, German Cancer Research Center DKFZ, Im Neuenheimer Feld 280, 69120 Heidelberg, Germany
    Free Radic Biol Med 43:1109-20. 2007
    ....
  44. ncbi The apoptotic effect of cordycepin on human OEC-M1 oral cancer cell line
    Wei Ciao Wu
    Department of Medicine, College of Medicine, National Cheng Kung University, Tainan, Taiwan, ROC
    Cancer Chemother Pharmacol 60:103-11. 2007
    ..05) in 12, 24 and 48 h cordycepin treatment. These results further confirmed the apoptotic event. In conclusion, cordycepin significantly induced cell apoptotsis in OEC-M1 human oral squamous cancer cells...
  45. ncbi Cordycepin inhibits lipopolysaccharide-induced inflammation by the suppression of NF-kappaB through Akt and p38 inhibition in RAW 264.7 macrophage cells
    Ho Gyoung Kim
    Department of Biochemistry, Kangwon National University, Chunchon 200 701, Republic of Korea Mushtech Co Ltd, Chunchon, Republic of Korea
    Eur J Pharmacol 545:192-9. 2006
    ..Thus, cordycepin may provide a potential therapeutic approach for inflammation-associated disorders...
  46. ncbi Purine 5',8-cyclonucleoside lesions: chemistry and biology
    Chryssostomos Chatgilialoglu
    ISOF, Consiglio Nazionale delle Ricerche, Via P Gobetti 101, 40129 Bologna, Italy
    Chem Soc Rev 40:1368-82. 2011
    ..Results reported for irradiated samples of calf thymus DNA have been critically reviewed, underlining the need of further research for the potential involvement of these lesions in human health (76 references)...
  47. ncbi Cordycepin/Hydroxyurea synergy allows low dosage efficacy of cordycepin in MOLT-4 leukemia cells
    Hania Wehbe-Janek
    Department of Biology, Institute of Biomedical Studies, Center for Drug Discovery, Baylor University, One Bear Place 97388, Waco, TX 76798, USA
    Anticancer Res 27:3143-6. 2007
    ..Cordycepin requires the relatively toxic co-drug, deoxycoformycin, for full efficacy as an anticancer agent. We sought to improve cordycepin efficacy using other, less toxic co-drugs...
  48. pmc Crystal structure of human spermine synthase: implications of substrate binding and catalytic mechanism
    Hong Wu
    Structural Genomics Consortium, University of Toronto, Toronto, Ontario M5G 1L5, Canada
    J Biol Chem 283:16135-46. 2008
    ..An analysis to trace possible evolutionary origins of spermine synthase is also described...
  49. ncbi 5'-methylthioadenosine modulates the inflammatory response to endotoxin in mice and in rat hepatocytes
    Henar Hevia
    División de Hepatología y Terapia Génica, Departamento de Medicina Interna, CIMA, Universidad de Navarra, Pamplona, Spain
    Hepatology 39:1088-98. 2004
    ..In conclusion, these observations demonstrate novel immunomodulatory properties for MTA that may be of value in the management of inflammatory diseases...
  50. ncbi Effects of sample preparation methods on the quantification of nucleosides in natural and cultured Cordyceps
    F Q Yang
    Institute of Chinese Medical Sciences, University of Macau, Macao SAR, China
    J Pharm Biomed Anal 48:231-5. 2008
    ..sinensis had no obvious variation among the three extraction methods. The results suggest that sample preparation has significant effect on the quantification of nucleosides in Cordyceps...
  51. ncbi Cordycepin causes p21WAF1-mediated G2/M cell-cycle arrest by regulating c-Jun N-terminal kinase activation in human bladder cancer cells
    Se Jung Lee
    Department of Food and Biotechnology, Chungju National University, Chungju, Chungbuk 380 702, South Korea
    Arch Biochem Biophys 490:103-9. 2009
    ..These results suggest that cordycepin could be an effective treatment for bladder cancer...
  52. pmc Structure of the 1,N2-etheno-2'-deoxyguanosine adduct in duplex DNA at pH 8.6
    Ganesh Shanmugam
    Department of Chemistry, Vanderbilt Institute of Chemical Biology, Center in Molecular Toxicology, and the Vanderbilt Ingram Cancer Center, Vanderbilt University, Nashville, Tennessee 37235, USA
    Chem Res Toxicol 20:1601-11. 2007
    ..The 5'-neighbor T(5).A(20) base pair was destabilized with respect to Watson-Crick base pairing. The refined structure predicted a bend in the helical axis associated with the adduct site...
  53. pmc 4'-C-methyl-2'-deoxyadenosine and 4'-C-ethyl-2'-deoxyadenosine inhibit HIV-1 replication
    B Christie Vu
    HIV Drug Resistance Program, NCI Frederick, P O Box B, Building 539, Frederick, MD 21702, USA
    Antimicrob Agents Chemother 55:2379-89. 2011
    ..Experiments that measure the ability of the compounds to block DNA synthesis in infected cells suggest that this differential block to DNA synthesis also occurs in infected cells...
  54. pmc Termination of DNA synthesis by N6-alkylated, not 3'-O-alkylated, photocleavable 2'-deoxyadenosine triphosphates
    Weidong Wu
    LaserGen, Inc, Houston, TX 77054, USA
    Nucleic Acids Res 35:6339-49. 2007
    ..A five-base experiment, illustrating the exquisite, stepwise addition through a homopolymer repeat, demonstrates the applicability of the N(6)-(2-nitrobenzyl)-dATP as an ideal RT for CRT sequencing...
  55. ncbi Large stabilization of a DNA duplex by the deoxyadenosine derivatives tethering an aromatic hydrocarbon group
    Shu ichi Nakano
    High Technology Research Center and Department of Chemistry, Faculty of Science and Engineering, Konan University, 8 9 1 Okamoto, Higashinada ku, Kobe 658 8501, Japan
    J Am Chem Soc 125:8086-7. 2003
    ....
  56. ncbi Repair of DNA containing Fapy.dG and its beta-C-nucleoside analogue by formamidopyrimidine DNA glycosylase and MutY
    Carissa J Wiederholt
    Department of Chemistry, Johns Hopkins University, 3400 North Charles Street, Baltimore, Maryland 21218, USA
    Biochemistry 42:9755-60. 2003
    ..The similar effects of Fapy.dG and 8-oxodG on DNA polymerase and repair enzymes in vitro raise the question as to whether Fapy.dG elicits similar effects in vivo...
  57. ncbi 2'-deoxy-4'-C-ethynyl-2-fluoroadenosine, a nucleoside reverse transcriptase inhibitor, is highly potent against all human immunodeficiency viruses type 1 and has low toxicity
    Hiroshi Ohrui
    Yokohama College of Pharmacy, 601 Matanocho, Tozukaku, Yokohama 245 0066, Japan
    Chem Rec 6:133-43. 2006
    ....
  58. ncbi Fapy.dG instructs Klenow exo(-) to misincorporate deoxyadenosine
    Carissa J Wiederholt
    Department of Chemistry, Colorado State University, Ft Collins, Colorado 80523, USA
    J Am Chem Soc 124:7278-9. 2002
    ..Overall, Fapy.dG increases the probability that Klenow exo- will make a mistake during replication approximately 80-million fold compared to a template containing the native nucleotide, dG...
  59. ncbi Toward a new genetic system with expanded dimensions: size-expanded analogues of deoxyadenosine and thymidine
    Haibo Liu
    Department of Chemistry, Stanford University, Stanford, California 94305 5080, USA
    J Am Chem Soc 126:1102-9. 2004
    ..Elements of the design of this expanded genetic molecule, termed xDNA, are discussed, including the possibility of up to eight base pairs of information storage capability...
  60. pmc Uncoupling of the base excision and nucleotide incision repair pathways reveals their respective biological roles
    Alexander A Ishchenko
    Groupe Réparation de l ADN, Unité Mixte de Recherche 8126, Centre National de la Recherche Scientifique, Institut Gustave Roussy, 94805 Villejuif Cedex, France
    Proc Natl Acad Sci U S A 103:2564-9. 2006
    ..Our data establish that NIR is a distinct and separable function of AP endonucleases essential for handling lethal oxidative DNA lesions...
  61. pmc Conformational interconversion of the trans-4-hydroxynonenal-derived (6S,8R,11S) 1,N(2)-deoxyguanosine adduct when mismatched with deoxyadenosine in DNA
    Hai Huang
    Department of Chemistry, Center in Molecular Toxicology, Center for Structural Biology and Vanderbilt Ingram Cancer Center, Vanderbilt University, Nashville, Tennessee 37235, USA
    Chem Res Toxicol 22:187-200. 2009
    ..A(18) base pair. The HNE aliphatic chain was oriented toward the minor groove. The Watson-Crick hydrogen bonding of the neighboring A(8).T(17) base pair was also disrupted...
  62. ncbi Site-selective RNA cleavage by DNA bearing a base pair-mimic nucleoside
    Shu ichi Nakano
    Frontier Institute for Biomolecular Engineering Research and Department of Chemistry, Faculty of Science and Engineering, Konan University, 8 9 1 Okamoto, Higashinada ku, Kobe 658 8501, Japan
    J Am Chem Soc 127:518-9. 2005
    ....
  63. ncbi 5'-Methylthioadenosine administration prevents lipid peroxidation and fibrogenesis induced in rat liver by carbon-tetrachloride intoxication
    M M Simile
    Department of Biomedical Sciences, University of Sassari, Italy
    J Hepatol 34:386-94. 2001
    ..CONCLUSIONS: MTA and SAM protect against oxidative liver injury through partially different mechanisms...
  64. pmc 8-vinyl-deoxyadenosine, an alternative fluorescent nucleoside analog to 2'-deoxyribosyl-2-aminopurine with improved properties
    Nouha Ben Gaied
    Laboratoire de Chimie Bioorganique, UMR 6001 du CNRS, Université de Nice Sophia Antipolis Parc Valrose 06108 Nice cedex 2, France
    Nucleic Acids Res 33:1031-9. 2005
    ..Taken together, our data suggest that 8vdA is a non perturbing nucleoside analog that may be used with improved sensitivity for the same applications as 2AP...
  65. ncbi Alpha-anomeric deoxynucleotides, anoxic products of ionizing radiation, are substrates for the endonuclease IV-type AP endonucleases
    Alexander A Ishchenko
    Groupe Réparation de l ADN, UMR 8126 CNRS, Institut Gustave Roussy, 94805 Villejuif Cedex, France
    Biochemistry 43:15210-6. 2004
    ..We propose that the novel substrate specificities of Nfo and Apn1 play an important role in counteracting oxidative DNA base damage...
  66. pmc Evidence for the involvement of DNA repair enzyme NEIL1 in nucleotide excision repair of (5'R)- and (5'S)-8,5'-cyclo-2'-deoxyadenosines
    Pawel Jaruga
    Chemical Science and Technology Laboratory, National Institute of Standards and Technology, Gaithersburg, Maryland 20899, USA
    Biochemistry 49:1053-5. 2010
    ..Further studies aimed at elucidating the mechanism of action of NEIL1 in NER are warranted...
  67. ncbi Methylthioadenosine
    Matias A Avila
    Division of Hepatology and Gene Therapy, F I M A, University of Navarra, Pamplona, Spain
    Int J Biochem Cell Biol 36:2125-30. 2004
    ..Finally, observations carried out in models of liver damage and cancer demonstrate a therapeutic potential for MTA that deserves further consideration...
  68. pmc Accumulation of (5'S)-8,5'-cyclo-2'-deoxyadenosine in organs of Cockayne syndrome complementation group B gene knockout mice
    Guldal Kirkali
    Chemical Science and Technology Laboratory, National Institute of Standards and Technology, Gaithersburg, MD 20899, USA
    DNA Repair (Amst) 8:274-8. 2009
    ..Thus, this study provides, for the first time, the evidence that CSB plays a role in the repair of the DNA helix-distorting tandem lesion S-cdA. Accumulation of unrepaired S-cdA in vivo may contribute to the pathology associated with CS...
  69. ncbi Alpha-deoxyadenosine, a major anoxic radiolysis product of adenine in DNA, is a substrate for Escherichia coli endonuclease IV
    H Ide
    Department of Polymer Science and Engineering, Kyoto Institute of Technology, Japan
    Biochemistry 33:7842-7. 1994
    ..The ability of endonuclease IV to recognize alpha-deoxyadenosine suggests that endonuclease IV is able to recognize a new class of DNA base lesions that is not recognized by other DNA N-glycosylases and AP endonucleases...
  70. pmc EFA (9-beta-D-erythrofuranosyladenine) is an effective salvage agent for methylthioadenosine phosphorylase-selective therapy of T-cell acute lymphoblastic leukemia with L-alanosine
    Ayse Batova
    Department of Pediatrics Hematology Oncology, University of California San Diego, San Diego, CA 92103 8447, USA
    Blood 107:898-903. 2006
    ..Collectively, these data indicate that EFA is an effective agent for salvaging MTAP+ cells from L-alanosine toxicity and is superior to MTA due to lower cytotoxicity...
  71. ncbi Effect of cordycepin (3'-deoxyadenosine) on hematogenic lung metastatic model mice
    Kazuki Nakamura
    Department of Pharmacology, Faculty of Pharmaceutical Sciences, Mukogawa Women s University, Nishinomiya, Hyogo 663 8179, Japan
    In Vivo 19:137-41. 2005
    ..These results suggest that cordycepin exerts an anti-metastatic action, in part, by inhibiting the invasiveness of mouse melanoma cells...
  72. ncbi Synthesis of 3-deaza-3-nitro-2'-deoxyadenosine
    Caroline Crey-Desbiolles
    LEDSS UMR5616 associé au CNRS, BP53, Universite Joseph Fourier, 38041 Grenoble, France
    Bioorg Med Chem 14:1935-41. 2006
    ..In the second route, the convertible nucleoside 6-O-(2,4,6-trimethylphenyl)-3-deaza-2'-deoxyadenosine (28) was used to introduce 6-NH2 group in the last step...
  73. ncbi 3-nitro-3-deaza-2'-deoxyadenosine as a versatile photocleavable 2'-deoxyadenosine mimic
    Caroline Crey-Desbiolles
    LEDSS, UMR5616 CNRS, ICMG FR 2607 BP53, Universite Joseph Fourier, 38041 Grenoble, France
    J Am Chem Soc 126:9532-3. 2004
    ..Photocleavage of the resulting oligonucleotide is highly efficient in single and double strands. Hybridization properties of NidA are very similar to those of deoxyadenosine...
  74. ncbi Inhibitory effects of cordycepin (3'-deoxyadenosine), a component of Cordyceps militaris, on human platelet aggregation induced by thapsigargin
    Hyun Jeong Cho
    Department of Biomedical Laboratory Science, College of Biomedical Science and Engineering, and Regional Research Center, Inje University, Gyungnam 621 749, Korea
    J Microbiol Biotechnol 17:1134-8. 2007
    ..Accordingly, our data demonstrated that cordycepin may have a beneficial effect on platelet aggregation-mediated thrombotic diseases through the [Ca2+]i-regulating system such as cGMP...
  75. ncbi 4,5-Epoxy-2(E)-decenal-induced formation of 1,N(6)-etheno-2'-deoxyadenosine and 1,N(2)-etheno-2'-deoxyguanosine adducts
    Seon Hwa Lee
    Center for Cancer Pharmacology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104 6160, USA
    Chem Res Toxicol 15:300-4. 2002
    ..These findings provide an important link between a primary product of lipid peroxidation and a mutagenic DNA lesion that has been detected in human tissues...
  76. ncbi S-adenosylmethionine and methylthioadenosine are antiapoptotic in cultured rat hepatocytes but proapoptotic in human hepatoma cells
    Eduardo Ansorena
    Departamento de Bioquimica, División de Hepatología y Terapia Génica, Departamento de Medicina Interna, Universidad de Navarra, Pamplona, Spain
    Hepatology 35:274-80. 2002
    ..These effects may participate in the hepatoprotective and chemopreventive properties of this safe and well-tolerated drug...
  77. ncbi Synthesis and pharmacokinetic evaluation of novel N-acyl-cordycepin derivatives with a normal alkyl chain
    H P Wei
    School of Pharmaceutical Science, Southwest University, Chongqing 400715, China
    Eur J Med Chem 44:665-9. 2009
    ..It indicated that N-octanoyl modification could decrease the metabolic velocity and increase the bioavailability of cordycepin to the maximum, thus it might be a promising prodrug of cordycepin...
  78. ncbi The role of cordycepin in cancer treatment via induction or inhibition of apoptosis: implication of polyadenylation in a cell type specific manner
    Hellinida Thomadaki
    Institute of Biology, NCSR Demokritos, Aghia Paraskevi Attikis, 15310 Athens, Greece
    Cancer Chemother Pharmacol 61:251-65. 2008
    ..In the present study we assessed the implication and role of cordycepin, a polyadenylation-specific inhibitor and a well-known chemotherapeutic drug, in apoptosis, induced by the anticancer drug etoposide...
  79. ncbi The oxidative DNA lesion 8,5'-(S)-cyclo-2'-deoxyadenosine is repaired by the nucleotide excision repair pathway and blocks gene expression in mammalian cells
    P J Brooks
    Laboratory of Neurogenetics, National Institute on Alcohol Abuse and Alcoholism, Bethesda, Maryland 20892, USA
    J Biol Chem 275:22355-62. 2000
    ..Based on these findings, we propose that cyclo-dA is a candidate for an endogenous DNA lesion that might contribute to neurodegeneration in XP...
  80. ncbi S-adenosylmethionine decarboxylase from Leishmania donovani. Molecular, genetic, and biochemical characterization of null mutants and overproducers
    Sigrid C Roberts
    Department of Biochemistry and Molecular Biology, Oregon Health and Science University, Portland, Oregon 97201 3098, USA
    J Biol Chem 277:5902-9. 2002
    ..The molecular, biochemical, and genetic characterization of ADOMETDC establishes that it is essential in L. donovani promastigotes and a potential target for therapeutic validation...
  81. pmc Cordycepin-hypersensitive growth links elevated polyphosphate levels to inhibition of poly(A) polymerase in Saccharomyces cerevisiae
    Sandra Holbein
    Institute of Molecular Biology, University of Zurich, Zurich, Switzerland
    Nucleic Acids Res 36:353-63. 2008
    ..Based on these observations, we suggest that binding of poly P to poly(A) polymerase negatively regulates its activity...
  82. pmc Mechanism of 1,N2-etheno-2'-deoxyguanosine formation from epoxyaldehydes
    Katya V Petrova
    Department of Chemistry and Center in Molecular Toxicology, Vanderbilt University, VU Station B 351822, Nashville, Tennessee 37235 1822, USA
    Chem Res Toxicol 20:1685-92. 2007
    ..Also observed are products that appear to be derived from 2,3-epoxyoctanal, which can be formed through Schiff base formation of 4,5-epoxy-2-decenal with the dGuo followed by hydration of the double bond and retro-aldol reaction...
  83. ncbi A highly facile and efficient one-step synthesis of N6-adenosine and N6-2'-deoxyadenosine derivatives
    Zhao Kui Wan
    Chemical and Screening Sciences, Wyeth Research, 200 Cambridge Park Drive, Cambridge, Massachusetts 02140, USA
    Org Lett 7:5877-80. 2005
    ..Carcinogenic polyaromatic hydrocarbon (PAH) N6-2'-deoxyadenosine adduct 10 and a rare DNA constituent 11 were thus synthesized directly from 2'-deoxyinosine both in 98% yield...
  84. ncbi Transcription inhibitors stimulate translation of 5' TOP mRNAs through activation of S6 kinase and the mTOR/FRAP signalling pathway
    F Loreni
    Dipartimento di Biologia, Universita Tor Vergata, Roma, Italy Department of Growth Control, Friedrich Miescher Institut, Basel, Switzerland
    Eur J Biochem 267:6594-601. 2000
    ..These results indicate that the effect of act D on translation is mediated by the S6Ks through FRAP/mTOR...
  85. pmc Genetic effects of oxidative DNA damages: comparative mutagenesis of the imidazole ring-opened formamidopyrimidines (Fapy lesions) and 8-oxo-purines in simian kidney cells
    M Abul Kalam
    Department of Chemistry, University of Connecticut Storrs, CT 06269, USA
    Nucleic Acids Res 34:2305-15. 2006
    ..dG or Fapy.dA site-specifically incorporated, which showed unequivocally that in simian kidney cells Fapy.G-->T substitutions occur at a higher frequency than 8-oxo-G-->T and that Fapy.dA is very weakly mutagenic, as is 8-oxo-dA...
  86. ncbi Inhibition of Werner syndrome helicase activity by benzo[a]pyrene diol epoxide adducts can be overcome by replication protein A
    Saba Choudhary
    Laboratory of Molecular Gerontology, National Institute on Aging, National Institutes of Health DHHS, Baltimore, MD 21224, USA
    J Biol Chem 281:6000-9. 2006
    ....
  87. ncbi Nucleoside and DNA adducts from N-nitrosotolazoline
    Richard N Loeppky
    Department of Medicinal Chemistry, University of Washington, Seattle, WA 98195, USA
    Chem Res Toxicol 21:319-29. 2008
    ....
  88. ncbi Simultaneous determination of O6-methyl-2'-deoxyguanosine, 8-oxo-7,8-dihydro-2'-deoxyguanosine, and 1,N6-etheno-2'-deoxyadenosine in DNA using on-line sample preparation by HPLC column switching coupled to ESI-MS/MS
    Andreas Brink
    Department of Toxicology, University of Wurzburg, Versbacher Str 9, D 97078 Wurzburg, Germany
    J Chromatogr B Analyt Technol Biomed Life Sci 830:255-61. 2006
    ..The method developed will be of use to study the biological significance of exogenous DNA adducts as an increment to background DNA damage and the role of modulating factors, such as DNA repair...
  89. doi Synthesis, characterization, and application of substituted pyrazolopyrimidine nucleosides
    Robert O Dempcy
    Epoch Biosciences, Bothell, Washington, USA
    Curr Protoc Nucleic Acid Chem . 2003
    ..Utility of this chemistry for preparing internally modified DNA conjugates is discussed...
  90. ncbi Synthesis and characterization of DNA adducts from the HIV reverse transcriptase inhibitor nevirapine
    Alexandra M M Antunes
    REQUIMTE CQFB, Faculdade de Ciencias e Tecnologia, Universidade Nova de Lisboa, 2829 516 Caparica, Portugal
    Chem Res Toxicol 21:1443-56. 2008
    ..If formed in vivo, some of these adducts would have considerable mutagenic potential. Our results thus suggest that NVP metabolism to 12-hydroxy-NVP may be a factor in NVP hepatocarcinogenicity...
  91. ncbi Detection of chlorinated DNA and RNA nucleosides by HPLC coupled to tandem mass spectrometry as potential biomarkers of inflammation
    Carine Badouard
    Laboratoire des Lésions des Acides Nucléiques, DRFMC SCIB CEA Grenoble, 17 rue des Martyrs, F 38054 Grenoble Cedex 9, France
    J Chromatogr B Analyt Technol Biomed Life Sci 827:26-31. 2005
    ..The method was found to be sensitive enough to allow the measurement of background levels of 5-chloro-2'-deoxycytidine in the DNA of human white blood cells isolated from 7 mL of blood...
  92. ncbi Silyl protecting groups for oligonucleotide synthesis removed by a ZnBr2 treatment
    Fernando Ferreira
    Laboratoire de Chimie Organique Biomoléculaire de Synthèse, UMR 5625 CNRS UM II, Université de Montpellier U1, Place E Bataillon, 34095 Montpellier Cedex 5, France
    Nucleosides Nucleotides Nucleic Acids 24:1009-13. 2005
    ..Finally it was released from the solid support by cleavage of a disulfide linkage with TCEP. The olgonucleotide was obtained without any basic treatment...
  93. pmc Highly diastereoselective synthesis of nucleoside adducts from the carcinogenic benzo[a]pyrene diol epoxide and a computational analysis
    Mahesh K Lakshman
    Department of Chemistry, The City College and The City University of New York, 138th Street at Convent Avenue, New York, New York 10031 9198, USA
    J Am Chem Soc 129:68-76. 2007
    ....
  94. ncbi Error-prone translesion synthesis by human DNA polymerase eta on DNA-containing deoxyadenosine adducts of 7,8-dihydroxy-9,10-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene
    Dominic Chiapperino
    Laboratory of Bioorganic Chemistry, NIDDK, National Institutes of Health, DHHS, Bethesda, Maryland 20892, USA
    J Biol Chem 280:39684-92. 2005
    ..This effect is largely independent of DNA sequence. Decreased mobility correlates with an increased rate of incorporation by pol eta, suggesting a systematic relationship between the overall DNA structure and efficiency of the enzyme...
  95. pmc Site-specific synthesis of oligonucleotides containing malondialdehyde adducts of deoxyguanosine and deoxyadenosine via a postsynthetic modification strategy
    Hao Wang
    Department of Chemistry and Biochemistry, Center in Molecular Toxicology and Vanderbilt Institute of Chemical Biology, Vanderbilt University, VU Station B 351822, Nashville, Tennessee 37235 1822, USA
    Chem Res Toxicol 19:1467-74. 2006
    ..The stability of the modified oligonucleotides was examined by UV thermal melting studies (Tm). In contrast to the M1dG adduct, OPdA caused very little change in the Tm...
  96. ncbi Effect of short-term fasting on urinary excretion of primary lipid peroxidation products and on markers of oxidative DNA damage in healthy women
    Kyoung Ho Lee
    Department of Preventive Medicine, Seoul National University College of Medicine, Institute for Environmental Medicine, SNUMRC, Seoul 110 799, Korea
    Carcinogenesis 27:1398-403. 2006
    ..51, n = 27, P = 0.02). Our results demonstrate that the short-term fasting reduces lipid peroxidation products but does not affect oxidative stress-induced DNA damage...
  97. ncbi Cytochrome P450 1B1-mediated estrogen metabolism results in estrogen-deoxyribonucleoside adduct formation
    Alexandra R Belous
    Department of Pathology, Vanderbilt University Medical Center, Nashville, Tennessee 37232, USA
    Cancer Res 67:812-7. 2007
    ..The results present direct proof of CYP1B1-mediated, E2-induced adduct formation and provide the experimental basis for future studies of estrogen carcinogenesis...
  98. ncbi Depurinating acylfulvene-DNA adducts: characterizing cellular chemical reactions of a selective antitumor agent
    Jiachang Gong
    Department of Medicinal Chemistry, College of Pharmacy, University of Minnesota, Minneapolis, Minnesota 55455, USA
    J Am Chem Soc 129:2101-11. 2007
    ..Moreover, a positive correlation was observed between DNA adduct levels and cell sensitivity to AF. The potential contributing roles of AOR-mediated bioactivation and adduct stability to the cytotoxicity of AF are discussed...
  99. pmc Formation of 1,4-dioxo-2-butene-derived adducts of 2'-deoxyadenosine and 2'-deoxycytidine in oxidized DNA
    Bingzi Chen
    Biological Engineering Division, Massachusetts Institute of Technology, 77 Massachusetts Avenue, NE47 277, Cambridge, Massachusetts 02139, USA
    Chem Res Toxicol 19:982-5. 2006
    ..These results suggest that the oxadiazabicyclo(3.3.0)octaimine adducts of dC and dA could represent endogenous DNA lesions arising from oxidative stresses that also give rise to other DNA adducts...
  100. ncbi Oxidation of vinyl carbamate and formation of 1,N6-ethenodeoxyadenosine in murine lung
    Poh Gek Forkert
    Department of Anatomy and Cell Biology, Queen s University, Kingston, Ontario, Canada K7L 3N6
    Drug Metab Dispos 35:713-20. 2007
    ..0 mM. These findings suggested that CYP2E1 is a major enzyme mediating VC oxidation, leading to the formation of a metabolite that alkylates DNA to form the epsilon dAS adduct...
  101. ncbi Site specific N6-(2-hydroxy-3,4-epoxybut-1-yl)adenine oligodeoxynucleotide adducts of 1,2,3,4-diepoxybutane: synthesis and stability at physiological pH
    Sergey Antsypovich
    Department of Medicinal Chemistry and The Cancer Center, University of Minnesota, Minneapolis, Minnesota 55455, USA
    Chem Res Toxicol 20:641-9. 2007
    ..These results indicate that DEB-induced alkylation of N6-adenine in DNA is unlikely to lead to DNA-DNA cross-linking but instead can result in the formation of exocyclic dA adducts...

Research Grants62

  1. DNA damage repair by MUTYH and MUTYH variants associated with colorectal cancer
    SHEILA SUE DAVID; Fiscal Year: 2013
    ..This information will provide insight into how MUTYH variations lead to dysfunction in DNA repair resulting in the accumulation of deleterious mutations, and thereby setting the stage for colorectal cancer. ..
  2. CHEMISTRY AND BIOLOGY OF MALONDIALDEHYDE DNA ADDUCTS
    Lawrence J Marnett; Fiscal Year: 2013
    ..abstract_text> ..
  3. Microbicide properties of RT inhibitor combinations
    Michael A Parniak; Fiscal Year: 2012
    ..R33 Deliverables: Identification of an appropriate delivery formulation for the selected NRTI/NNRTI combination for entry into subsequent preclinical safety and efficacy studies. ..
  4. Ultrapotent Inhibitors of Wild-type and Multi-drug Resistant HIV
    Stefan Sarafianos; Fiscal Year: 2012
    ....
  5. MECHANISMS OF ANTIFOLATE EFFICACY IN ARTHRITIS
    Sarah Morgan; Fiscal Year: 2000
    ..The information obtained from the proposed research will enhance the understanding of the biochemical action of antifolates/antimetabolites that are effective in the treatment of human and animal arthritis. ..
  6. AdoHcy Hydrolase Inhibitors as Antiparasitic Agents
    RONALD BORCHARDT; Fiscal Year: 2006
    ..The outcome of this research program should be the identification of specific inhibitors of parasite AdoHcy hydiolases that have clinical potential as antiparasitic agents. ..
  7. PHARMACOLOGY OF DIDEOXYNUCLEOSIDES IN SMALL INTESTINAL
    JESSIE AU; Fiscal Year: 1991
    ..Examine the effect of the combination on the cytotoxicity, the metabolite kinetics, and the effect of treatment conditions (e.g., simultaneously or sequentially)...
  8. Catalytic Mechanism of Biotin Synthase
    JOSEPH JARRETT; Fiscal Year: 2007
    ..abstract_text> ..
  9. ADENOSINE DEAMINASE--TARGET IN MOLECULAR TERATOGENESIS
    Thomas Knudsen; Fiscal Year: 1993
    ..The expression of ADA at the maternal/embryonal interface is a target that can be studied by modern, sensitive molecular methods in attempting to define the cause of abnormal embryonic development...
  10. Adenosine Deaminase, Adenosine Signaling and Disease
    RODNEY KELLEMS; Fiscal Year: 2006
    ..What is the impact of ADA deficiency on natural killer cell development and function? AIM IV. What role does adenosine signaling play in the non-immune features associated with ADA deficiency? ..
  11. ADENOSINE DEAMINASE DEFICIENCY
    Linda Thompson; Fiscal Year: 2007
    ..Our data will contribute to the growing knowledge base needed to understand and control the vulnerability of the developing human immune system to environmental threats and infectious agents. ..
  12. MINORITY PREDOCTORAL FELLOWSHIP PROGRAM
    NATASHA NESBITT; Fiscal Year: 2005
    ..Lastly, analogues of AdoMet with the potential to stabilize a radical at the 5'-carbon of 5'-deoxyadenosine will be used to establish the involvement of radical intermediates in the LAS reaction. ..
  13. DNA BASE SEQUENCE EFFECTS IN CHEMICAL CARCINOGENESIS
    Nicholas Geacintov; Fiscal Year: 2002
    ....
  14. CORE MASS SPECTROMETRY RESOURCE FOR CANCER RESEARCH
    Richard van Breemen; Fiscal Year: 2003
    ..abstract_text> ..
  15. Transgenic Mouse Model for Adenosine-induced Asthma
    Tehsheng Chan; Fiscal Year: 2003
    ..This transgenic mouse line provides a unique opportunity for studying the mechanism of Th2 cell-independent asthma-like disease, and has great potential for use in developing novel therapeutics. ..
  16. RIBONUCLEOTIDE REDUCTASE AS A CHEMOTHERAPEUTIC TARGET
    JOSEPH CORY; Fiscal Year: 1990
    ....
  17. ANTITUMOR DRUG DEVELOPMENT
    ROBERT ABELES; Fiscal Year: 1980
    ..Fluoroaspartate is extremely cytotoxic to certian cell lines. Some indications are available that it might inhibit or inactivate TRNA charging enzymes. We will continue an investigation of the mechanism of action of this compound...
  18. MOLECULAR PATHOLOGY OF INHERITED IMMUNODEFICIENCY
    Tehsheng Chan; Fiscal Year: 1993
    ..Finally, the animal model will be valuable in the development of gene therapy of ADA deficiency in humans...
  19. MOLECULAR RADIOBIOLOGY OF NUCLEIC ACIDS
    James Raleigh; Fiscal Year: 1990
    ....
  20. Microbicide properties of RT inhibitor combinations
    MICHAEL PARNIAK; Fiscal Year: 2009
    ..Our studies will provide potent new formulations to the microbicide development pipeline for entry into clinical evaluation. ..
  21. Purine Analog Anti-Mycobacterial Drug Development
    William Parker; Fiscal Year: 2009
    ..tb activity;and (4) design and synthesis of purine and purine nucleoside analogs with selective activity against M. tb. ..
  22. ELECTRON PARAMAGNETIC RESONANCE SPECTROMETER
    Kurt Warncke; Fiscal Year: 2003
    ..A sustained high impact of the EPR spectrometer on biomedicine is therefore anticipated. ..
  23. ENZYME CATALYSIS OF ELECTRON AND GROUP TRANSFER
    PERRY FREY; Fiscal Year: 2006
    ..The contributions of beta- amino acids produced by lysine 2,3-aminomutase and arginine 2,3-aminomutase to the functions of antibiotics are not known. The beta-aminoacids may become significant in drug development. ..
  24. MECHANISMS OF ENZYMATIC REACTIONS
    PERRY FREY; Fiscal Year: 2006
    ..unreadable] [unreadable]..
  25. Co(II)-Radical Pair Dynamics in B12 Enzyme Catalysis
    Kurt Warncke; Fiscal Year: 2008
    ..abstract_text> ..
  26. IMP Dehydrogenase and the Hydra of Cancer Chemotherapy
    GEORGE MARKHAM; Fiscal Year: 2008
    ..These results will reveal the inner working of an essential enzyme and whether there is promise for enhancing clinical efficacy through combinations of known drugs or the discovery of novel subdomain ligands. ..
  27. Molecular Models of Adenosine Signaling and Asthma
    Michael Blackburn; Fiscal Year: 2008
    ..These studies will lead to a better understanding of adenosine's impact on the severity of chronic lung diseases such as asthma and COPD, which will help guide the development of novel therapies for their treatment. ..
  28. T CELL KINETICS AND SOURCES--EFFECTS OF HIV AND THERAPY
    Marc Hellerstein; Fiscal Year: 2002
    ....
  29. Id2 REGULATION OF MUSCLE HYPERTROPHY IN AGING
    STEPHEN ALWAY; Fiscal Year: 2007
    ..abstract_text> ..
  30. Biosynthesis of the Antibiotic Polyketide Enterocin
    BRADLEY MOORE; Fiscal Year: 2005
    ..abstract_text> ..
  31. EQUILIBRIUM CYCLIZATION AS A PROBE OF DNA FLEXIBILITY
    PHILIP BOLTON; Fiscal Year: 2002
    ..The effects of magnesium, temperature and netropsin on the curvature, flexibility and helical repeat of dA tracts will be examined. ..
  32. ENZYMATIC MECHANISMS OF SULFUR NUCLEOSIDE METABOLISM
    GEORGE MARKHAM; Fiscal Year: 2008
    ..These studies will unmask why nature conserves use of a pyruvoyl cofactor for this metabolic function instead of adopting the common pyridoxal cofactor. ..
  33. ENDOCANNABINOID BIOMARKERS OF OBESITY USING INTEGRATED GENOMICS AND METABOLOMICS
    JACK SIPE; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  34. FAAH GENE MUTATIONS: RISK FACTORS IN DRUG USE/ADDICTION
    JACK SIPE; Fiscal Year: 2006
    ..abstract_text> ..
  35. Ring-Opened and Tandem DNA Damages
    Ashis K Basu; Fiscal Year: 2010
    ..g., isogenic mouse embryonic fibroblasts and cells that express modulated levels of DNA polymerases encoded by REV1, REV3, RAD18, etc. genes). We hope to contribute toward a deeper insight of the effects of oxidative DNA damages. ..
  36. Response to and signals of caloric restriction and intermittent feeding regimens
    MARC KOPEL HELLERSTEIN; Fiscal Year: 2010
    ....
  37. The impact of early antiretroviral therapy on HIV persistence and inflammation
    STEVEN GRANT DEEKS; Fiscal Year: 2010
    ..Our work may also provide important insights in the role of chronic inflammation in driving viral persistence, and hence may lead to the development of novel interventions. ..
  38. MYOD AND MYOGENIN IN MUSCLE HYPERTROPHY AND SARCOPENIA
    STEPHEN ALWAY; Fiscal Year: 2001
    ....
  39. ADENOSINE SIGNALING AND LUNG INFLAMMATION
    Michael Blackburn; Fiscal Year: 2001
    ..4. Evaluate acute and long-term changes of airway function in ADA deficient mice as well as ADA deficient mice exposed to environmental challenges. ..
  40. Combined Substrate Polymerase Inhibitors
    MICHAEL DOUGHTY; Fiscal Year: 2007
    ..These targets include DNA polymerase, reverse transcriptases, viral RNA dependent DNA polymerases, and inosine monophosphate dehydrogenase, a control enzyme in the cell synthesis of GMP. [unreadable] [unreadable] [unreadable]..
  41. INDEPENDENT SCIENTIST AWARD (NIEHS)
    ASHIS BASU; Fiscal Year: 2002
    ..Another important aim is to understand the mechanism of how the repair proteins protect cells against the toxic and mutagenic properties of these DNA damaging agents. ..
  42. Acquisition of a linear Q-Trap LC/MS/MS System
    Ian Blair; Fiscal Year: 2004
    ..Therefore, the new instrument would impact significantly on research programs of some 38-48 NIH-funded investigators at the University of Pennsylvania. ..
  43. Treatment Intensification for Drug-Resistant HIV
    Steven Deeks; Fiscal Year: 2004
    ..Funding for the pilot clinical trial has been obtained from other sources; funding is requested in this proposal only for costs associated with the measurements of immunologic response to drug-resistant HIV-1. ..
  44. DIRECTLY MEASURED T CELL DYNAMICS IN HIV PATHOGENESIS
    Marc Hellerstein; Fiscal Year: 2004
    ..Results have potential relevance to HIV-1 pathogenesis and therapy and to vaccination strategies. ..
  45. Fourier Transform Mass Spec Proteomics and Genomics
    Ian Blair; Fiscal Year: 2004
    ..Thus, while each investigator's research proposal stands alone in scientific value and integrity, the added value of this combined proposal is substantial. ..
  46. Immunologic Control of Drug-resistant HIV-1
    Steven Deeks; Fiscal Year: 2005
    ..Finally, since we will estimate the relative in vivo thresholds for HIV-mediated immunogenicity and pathogenicity, these studies may also have implications for vaccine development. ..
  47. High resolution triple quadrupole mass spectrometer
    Ian Blair; Fiscal Year: 2005
    ..Therefore, the new instrument would impact significantly on research programs of some 40-50 NIH-funded investigators at the University of Pennsylvania. ..
  48. ENDOGENOUS NITROSATION CHEMISTRY IN CARCINOGENESIS
    RICHARD LOEPPKY; Fiscal Year: 2004
    ..Computational methods will be employed to reveal reaction paths of intermediates and lead to better predictions of the types of compounds which pose a carcinogenic threat through their endogenous nitrosation. ..
  49. Adjuvanted Epitope Vaccine to Target HIV Reservoirs
    Michael Murphey Corb; Fiscal Year: 2006
    ..Together, these studies will both define the nature of viral persistence during chronic infection and identify an effective method of treatment. ..
  50. A Novel Scrambled Antigen DNA Vaccine (SAVINE) Strategy
    Michael Murphey Corb; Fiscal Year: 2003
    ..Together, these experiments will provide data essential to the translation of these studies to human trials. ..
  51. An Adjuvanted Therapeutic DNA Vaccine for AIDS
    Michael Murphey Corb; Fiscal Year: 2006
    ..abstract_text> ..
  52. ADIPOCYTE TURNOVER AND METABOLISM IN HIV-1 LIPOATROPHY
    Marc Hellerstein; Fiscal Year: 2003
    ....
  53. A LYMPHOBLAST MODEL FOR DISEASES OF PURINE METABOLISM
    Michael Hershfield; Fiscal Year: 2006
    ..abstract_text> ..
  54. Type III Polyketide Synthases: Structure and Mechanism
    BRADLEY MOORE; Fiscal Year: 2006
    ..abstract_text> ..
  55. RNA-regulation and signal transduction by PKR
    CYNTHIA BURROWS; Fiscal Year: 2006
    ..These will include studies designed to the correlate the relative positions of binding sites for PKR's two dsRBMs on an RNA molecule with that RNA's efficacy as a kinase activator. ..
  56. MECHANISM OF ENZYME MEDIATED ACTIVATION OF COENZYME B12
    Kenneth Brown; Fiscal Year: 2005
    ..In addition, the possibility that the active species of RTPR is actually a dimer will be investigated by determining the effect of protein concentration on the observed binding constants for AdoCbl and the allosteric effector dGTP. ..
  57. Effects of Formamidopyrimidine Lesions on DNA
    Marc Greenberg; Fiscal Year: 2006
    ..abstract_text> ..
  58. DbpA/B proteins of Borrelia burgdorferi & Lyme arthritis
    Nikhat Parveen; Fiscal Year: 2005
    ..This study will characterize the role of two spirochete lipoprotein adhesins in Lyme arthritis in the murine model. ..
  59. Symposium on Proteomics and Toxicology
    Ian Blair; Fiscal Year: 2003
    ..It is anticipated that the symposium will also provide the opportunity to introduce new investigators to the field. ..