dna primers

Summary

Summary: Short sequences (generally about 10 base pairs) of DNA that are complementary to sequences of messenger RNA and allow reverse transcriptases to start copying the adjacent sequences of mRNA. Primers are used extensively in genetic and molecular biology techniques.

Top Publications

  1. ncbi Primer3 on the WWW for general users and for biologist programmers
    S Rozen
    Whitehead Institute for Biomedical Research, Cambridge, MA, USA
    Methods Mol Biol 132:365-86. 2000
  2. pmc A new mathematical model for relative quantification in real-time RT-PCR
    M W Pfaffl
    Institute of Physiology, FML Weihenstephan, Center of Life and Food Sciences, Technical University of Munich, Germany
    Nucleic Acids Res 29:e45. 2001
  3. ncbi DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates
    O Folmer
    Center for Theoretical and Applied Genetics, Rutgers University, New Brunswick, New Jersey 08903 231
    Mol Mar Biol Biotechnol 3:294-9. 1994
  4. pmc Primer3Plus, an enhanced web interface to Primer3
    Andreas Untergasser
    Laboratory of Molecular Biology, Department of Plant Science, Wageningen University, Dreijenlaan 3, 6703HA, Wageningen, The Netherlands
    Nucleic Acids Res 35:W71-4. 2007
  5. ncbi Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays
    S A Bustin
    Academic Department of Surgery, St Bartholomew s and the Royal London School of Medicine and Dentistry, Queen Mary and Westfield College, London E1 1BB, UK
    J Mol Endocrinol 25:169-93. 2000
  6. ncbi MethPrimer: designing primers for methylation PCRs
    Long Cheng Li
    Department of Urology, Veterans Affairs Medical Center, and University of California San Francisco, San Francisco, CA 94121, USA
    Bioinformatics 18:1427-31. 2002
  7. pmc Ten species in one: DNA barcoding reveals cryptic species in the neotropical skipper butterfly Astraptes fulgerator
    Paul D N Hebert
    Department of Zoology, University of Guelph, Guelph, ON, N1G 2W1, Canada
    Proc Natl Acad Sci U S A 101:14812-7. 2004
  8. ncbi Guideline to reference gene selection for quantitative real-time PCR
    Aleksandar Radonic
    Charité Campus Charité Mitte, II Medizinische Klinik mit Schwerpunkt Onkologie und Hämatologie, Humboldt Universitat, Berlin, Germany
    Biochem Biophys Res Commun 313:856-62. 2004
  9. pmc Preservation of duplicate genes by complementary, degenerative mutations
    A Force
    Department of Biology, University of Oregon, Eugene, Oregon 97403, USA
    Genetics 151:1531-45. 1999
  10. ncbi An economic method for the fluorescent labeling of PCR fragments
    M Schuelke
    Department of Neuropediatrics, Charite University Hospital, Augustenburger Platz 1, D 13353 Berlin, Germany
    Nat Biotechnol 18:233-4. 2000

Research Grants

  1. Amplifying mRNA Sequences from Small Amounts of Tissue
    Paul Siebert; Fiscal Year: 2006
  2. Providing the $1000 genome via improved single molecule sequencing
    JOHN FENTON THOMPSON; Fiscal Year: 2010
  3. DNA sequencing by ELFSE on microfluidic devices
    Annelise Barron; Fiscal Year: 2006
  4. ADDRESSING THE ISSUE OF GAP REGIONS IN GENOME SEQUENCING
    John SantaLucia; Fiscal Year: 2001
  5. Novel Real Time Isothermal DNA Amplification/Detection
    WILLIAM MACCONNELL; Fiscal Year: 2005
  6. POST-TRANSCRIPTIONAL MODIFICATIONS IN RIBOSOMAL RNA
    David Jemiolo; Fiscal Year: 1990
  7. DNA BASE CALLING USING HETERODYNE FLUORESCENCE DETECTION
    Kristen Peterson; Fiscal Year: 1999
  8. Novel Real Time Isothermal DNA Amplification/Detection
    WILLIAM MACCONNELL; Fiscal Year: 2003
  9. INT-1 PROTO-ONCOGENE FAMILY
    GREGORY SHACKLEFORD; Fiscal Year: 1993
  10. Multiplexed Reactive Sequencing of DNA
    Peter Williams; Fiscal Year: 2007

Detail Information

Publications275 found, 100 shown here

  1. ncbi Primer3 on the WWW for general users and for biologist programmers
    S Rozen
    Whitehead Institute for Biomedical Research, Cambridge, MA, USA
    Methods Mol Biol 132:365-86. 2000
  2. pmc A new mathematical model for relative quantification in real-time RT-PCR
    M W Pfaffl
    Institute of Physiology, FML Weihenstephan, Center of Life and Food Sciences, Technical University of Munich, Germany
    Nucleic Acids Res 29:e45. 2001
    ..High accuracy and reproducibility (<2.5% variation) were reached in LightCycler PCR using the established mathematical model...
  3. ncbi DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates
    O Folmer
    Center for Theoretical and Applied Genetics, Rutgers University, New Brunswick, New Jersey 08903 231
    Mol Mar Biol Biotechnol 3:294-9. 1994
    We describe "universal" DNA primers for polymerase chain reaction (PCR) amplification of a 710-bp fragment of the mitochondrial cytochrome c oxidase subunit I gene (COI) from 11 invertebrate phyla: Echinodermata, Mollusca, Annelida, ..
  4. pmc Primer3Plus, an enhanced web interface to Primer3
    Andreas Untergasser
    Laboratory of Molecular Biology, Department of Plant Science, Wageningen University, Dreijenlaan 3, 6703HA, Wageningen, The Netherlands
    Nucleic Acids Res 35:W71-4. 2007
    ..The Primer3Plus Perl source code is available under GPL license from SourceForge. Primer3Plus is available at http://www.bioinformatics.nl/primer3plus...
  5. ncbi Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays
    S A Bustin
    Academic Department of Surgery, St Bartholomew s and the Royal London School of Medicine and Dentistry, Queen Mary and Westfield College, London E1 1BB, UK
    J Mol Endocrinol 25:169-93. 2000
    ..It illustrates the usefulness of these assays by demonstrating the significantly different levels of transcription between individuals of the housekeeping gene family, glyceraldehyde-3-phosphate-dehydrogenase (GAPDH)...
  6. ncbi MethPrimer: designing primers for methylation PCRs
    Long Cheng Li
    Department of Urology, Veterans Affairs Medical Center, and University of California San Francisco, San Francisco, CA 94121, USA
    Bioinformatics 18:1427-31. 2002
    ..Therefore, the present study was designed to develop a program for such applications...
  7. pmc Ten species in one: DNA barcoding reveals cryptic species in the neotropical skipper butterfly Astraptes fulgerator
    Paul D N Hebert
    Department of Zoology, University of Guelph, Guelph, ON, N1G 2W1, Canada
    Proc Natl Acad Sci U S A 101:14812-7. 2004
    ..They also illustrate the value of DNA barcoding, especially when coupled with traditional taxonomic tools, in disclosing hidden diversity...
  8. ncbi Guideline to reference gene selection for quantitative real-time PCR
    Aleksandar Radonic
    Charité Campus Charité Mitte, II Medizinische Klinik mit Schwerpunkt Onkologie und Hämatologie, Humboldt Universitat, Berlin, Germany
    Biochem Biophys Res Commun 313:856-62. 2004
    ..Our results show that "Classical" reference genes are indeed unsuitable, whereas the RNA polymerase II gene was the gene with the most constant expression in different tissues and following stimulation in CCRF-HSB-2 cells...
  9. pmc Preservation of duplicate genes by complementary, degenerative mutations
    A Force
    Department of Biology, University of Oregon, Eugene, Oregon 97403, USA
    Genetics 151:1531-45. 1999
    ..Cooke et al. 1997 (p. 362)..
  10. ncbi An economic method for the fluorescent labeling of PCR fragments
    M Schuelke
    Department of Neuropediatrics, Charite University Hospital, Augustenburger Platz 1, D 13353 Berlin, Germany
    Nat Biotechnol 18:233-4. 2000
    ..A poor man's approach to genotyping for research and high-throughput diagnostics...
  11. pmc Real-time quantification of microRNAs by stem-loop RT-PCR
    Caifu Chen
    Applied Biosystems, 850 Lincoln Centre Drive, Foster City, CA 94404, USA
    Nucleic Acids Res 33:e179. 2005
    ..Furthermore, the concept of stem-loop RT primer design could be applied in small RNA cloning and multiplex assays for better specificity and efficiency...
  12. pmc An essential role for interferon gamma in resistance to Mycobacterium tuberculosis infection
    J L Flynn
    Howard Hughes Medical Institute, Department of Microbiology, Albert Einstein College of Medicine, Bronx, New York 10461
    J Exp Med 178:2249-54. 1993
    ..In contrast to control mice, gko mice exhibit heightened tissue necrosis and succumb to a rapid and fatal course of tuberculosis that could be delayed, but not prevented, by treatment with exogenous recombinant IFN-gamma...
  13. pmc Methylation-specific PCR: a novel PCR assay for methylation status of CpG islands
    J G Herman
    Oncology Center, Johns Hopkins Medical Institutions, Baltimore, MD 21231, USA
    Proc Natl Acad Sci U S A 93:9821-6. 1996
    ....
  14. ncbi Design and standardization of PCR primers and protocols for detection of clonal immunoglobulin and T-cell receptor gene recombinations in suspect lymphoproliferations: report of the BIOMED-2 Concerted Action BMH4-CT98-3936
    J J M van Dongen
    Department of Immunology, Erasmus MC, University Medical Center Rotterdam, Rotterdam, The Netherlands
    Leukemia 17:2257-317. 2003
    ..The BIOMED-2 multiplex tubes can now be used for diagnostic clonality studies as well as for the identification of PCR targets suitable for the detection of minimal residual disease...
  15. ncbi Review and re-analysis of domain-specific 16S primers
    G C Baker
    Department of Biotechnology, University of the Western Cape, Bellville 7335, Cape Town, South Africa
    J Microbiol Methods 55:541-55. 2003
    ..These primers are found to amplify sequences from Crenarchaeote and Euryarchaeote type strains and environmental DNA...
  16. pmc Conservative fragments in bacterial 16S rRNA genes and primer design for 16S ribosomal DNA amplicons in metagenomic studies
    Yong Wang
    KAUST Global Partnership Program, Department of Biology, Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong, China
    PLoS ONE 4:e7401. 2009
    ..The primers recommended in this report shall facilitate a comprehensive and reliable survey of bacterial diversity in metagenomic studies...
  17. ncbi Light activation of channelrhodopsin-2 in excitable cells of Caenorhabditis elegans triggers rapid behavioral responses
    Georg Nagel
    Max Planck Institute for Biophysics, Max von Laue Str 3, D 60438 Frankfurt, Germany
    Curr Biol 15:2279-84. 2005
    ..Thus, specific neurons or muscles expressing ChR2 can be quickly and reversibly activated by light in live and behaving, as well as dissected, animals...
  18. pmc Short pyrosequencing reads suffice for accurate microbial community analysis
    Zongzhi Liu
    Department of Chemistry and Biochemistry, UCB 215, University of Colorado at Boulder, Boulder, CO 80309 0215, USA
    Nucleic Acids Res 35:e120. 2007
    ....
  19. ncbi Facile means for quantifying microRNA expression by real-time PCR
    Rui Shi
    North Carolina State University, Raleigh, NC 27695 7247, USA
    Biotechniques 39:519-25. 2005
    ..This method also revealed miRNA tissue-specific expression patterns that cannot be resolved by Northern blot analysis and may therefore be widely useful for characterizing miRNA expression in plants as well as in animals...
  20. ncbi Detection of loop-mediated isothermal amplification reaction by turbidity derived from magnesium pyrophosphate formation
    Y Mori
    Eiken Chemical Company, Ltd, 1381 3 Shimoishigami, Ohtawara, Tochigi 324 0036, Japan
    Biochem Biophys Res Commun 289:150-4. 2001
    ....
  21. pmc PrimerBank: a resource of human and mouse PCR primer pairs for gene expression detection and quantification
    Athanasia Spandidos
    Department of Genetics, Harvard Medical School, Center for Computational and Integrative Biology, Massachusetts General Hospital, Boston, MA 02114 2790, USA
    Nucleic Acids Res 38:D792-9. 2010
    ..In all, 26,855 primer pairs covering most known mouse genes have been experimentally validated by QPCR, agarose gel analysis, sequencing and BLAST, and all validation data can be freely accessed from the PrimerBank web site...
  22. pmc Improved amplification of genital human papillomaviruses
    P E Gravitt
    Department of Human Genetics, Roche Molecular Systems, Alameda, California, USA
    J Clin Microbiol 38:357-61. 2000
    ..This new primer system will be useful in assessing the natural history of HPV infections, particularly when the analysis requires HPV typing...
  23. ncbi PerlPrimer: cross-platform, graphical primer design for standard, bisulphite and real-time PCR
    Owen J Marshall
    Murdoch Childrens Research Institute, Royal Childrens Hospital, Flemington Road, Parkville, Vic, 3052, Australia
    Bioinformatics 20:2471-2. 2004
    ..It aims to automate and simplify the process of primer design...
  24. pmc An efficient one-step site-directed deletion, insertion, single and multiple-site plasmid mutagenesis protocol
    Huanting Liu
    SSPF, Centre for Biomolecular Science, University of St Andrews, North Haugh, St Andrews KY16 9ST, UK
    BMC Biotechnol 8:91. 2008
    ..Although many site-directed mutagenesis methods have been developed, a simple, quick and multi-applicable method is still desirable...
  25. ncbi Dual roles for glucokinase in glucose homeostasis as determined by liver and pancreatic beta cell-specific gene knock-outs using Cre recombinase
    C Postic
    Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
    J Biol Chem 274:305-15. 1999
    ..Interestingly, hepatic GK knock-out mice also have impaired insulin secretion in response to glucose. These studies indicate that deficiencies in both beta cell and hepatic GK contribute to the hyperglycemia of MODY-2...
  26. ncbi Molecular beacons: probes that fluoresce upon hybridization
    S Tyagi
    Department of Molecular Genetics, Public Health Research Institute, New York, NY 10016, USA
    Nat Biotechnol 14:303-8. 1996
    ..When introduced into living cells, these probes should enable the origin, movement, and fate of specific mRNAs to be traced...
  27. pmc Rapid amplification of plasmid and phage DNA using Phi 29 DNA polymerase and multiply-primed rolling circle amplification
    F B Dean
    Molecular Staging, Inc, New Haven, Connecticut 06511, USA
    Genome Res 11:1095-9. 2001
    ..Amplified products can also be used for in vitro cloning, library construction, and other molecular biology applications...
  28. pmc The repertoire for pattern recognition of pathogens by the innate immune system is defined by cooperation between toll-like receptors
    A Ozinsky
    Department of Immunology, University of Washington, H 574 Health Sciences, Box 357650, Seattle, WA 98195, USA
    Proc Natl Acad Sci U S A 97:13766-71. 2000
    ....
  29. ncbi Experimental factors affecting PCR-based estimates of microbial species richness and evenness
    Anna Engelbrektson
    Department of Energy Joint Genome Institute, Walnut Creek, CA, USA
    ISME J 4:642-7. 2010
    ..However, estimates of species evenness are consistent among different primer pairs targeting the same region. These results highlight the importance of experimental methodology when comparing diversity estimates across communities...
  30. ncbi Loop-mediated isothermal amplification (LAMP) of gene sequences and simple visual detection of products
    Norihiro Tomita
    Eiken Chemical Co, Ltd, Biochemical Research Laboratory, 1381 3 Shimoishigami, Ohtawara, Tochigi 324 0036, Japan
    Nat Protoc 3:877-82. 2008
    ..This detection method should be helpful in basic research on medicine and pharmacy, environmental hygiene, point-of-care testing and more...
  31. ncbi Accelerated reaction by loop-mediated isothermal amplification using loop primers
    K Nagamine
    Eiken Chemical Co Ltd 1381 3 Shimoishigami, Ohtawara, Tochigi 324 0036, Japan
    Mol Cell Probes 16:223-9. 2002
    ..Since the total time of analysis including detection is less than 1h, this new method should facilitate genetic analysis, including genetic diagnosis in the clinical laboratory...
  32. pmc DNA barcoding Australia's fish species
    Robert D Ward
    CSIRO Marine and Atmospheric Research, GPO Box 1538, Hobart, Tasmania 7001, Australia
    Philos Trans R Soc Lond B Biol Sci 360:1847-57. 2005
    ..We conclude that cox1 sequencing, or 'barcoding', can be used to identify fish species...
  33. pmc An efficient one-step site-directed and site-saturation mutagenesis protocol
    Lei Zheng
    Department of Chemistry and Biochemistry, University of Berne, Freiestrasse 3, CH 3012 Berne, Switzerland
    Nucleic Acids Res 32:e115. 2004
    ..This method should be useful to facilitate the preparation of high-quality site saturation libraries...
  34. ncbi Targeted deficiency or cytosolic truncation of the VE-cadherin gene in mice impairs VEGF-mediated endothelial survival and angiogenesis
    P Carmeliet
    Center for Transgene Technology and Gene Therapy, Flanders Interuniversity Institute for Biotechnology, Leuven, Belgium
    Cell 98:147-57. 1999
    ..Thus, VE-cadherin/ beta-catenin signaling controls endothelial survival...
  35. pmc Critical evaluation of two primers commonly used for amplification of bacterial 16S rRNA genes
    Jeremy A Frank
    Department of Microbiology, University of Illinois at Urbana Champaign, B103 C and LSL, 601 South Goodwin Ave, Urbana, IL 61801, USA
    Appl Environ Microbiol 74:2461-70. 2008
    ..particularly under stringent amplification conditions. Because our 27f formulation remains relatively simple, having seven distinct primer sequences, there is minimal loss of overall amplification efficiency and specificity...
  36. pmc Sensitive, seminested PCR amplification of VP1 sequences for direct identification of all enterovirus serotypes from original clinical specimens
    W Allan Nix
    Polio and Picornavirus Laboratory Branch, Division of Viral Diseases, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA
    J Clin Microbiol 44:2698-704. 2006
    ....
  37. ncbi Development and mapping of 2240 new SSR markers for rice (Oryza sativa L.)
    Susan R McCouch
    Plant Breeding Dept, Cornell University, Ithaca, NY 14853 1901, USA
    DNA Res 9:199-207. 2002
    ..In combination with the pool of 500 previously mapped SSR markers, this release makes available a total of 2740 experimentally confirmed SSR markers for rice, or approximately one SSR every 157 kb...
  38. pmc Assessment of soil microbial community structure by use of taxon-specific quantitative PCR assays
    Noah Fierer
    Department of Biology, Duke University, Durham, North Carolina 27708, USA
    Appl Environ Microbiol 71:4117-20. 2005
    ..Primers were thoroughly tested for specificity, and the method was applied to three distinct soils. The technique provides a rapid and robust index of microbial community structure...
  39. pmc A third-generation lentivirus vector with a conditional packaging system
    T Dull
    Cell Genesys, Foster City, California 94404, USA
    J Virol 72:8463-71. 1998
    ..While the actual biosafety of the vector will ultimately be proven in vivo, the improved design presented here should facilitate testing of lentivirus vectors...
  40. pmc Identification of mammalian microRNA host genes and transcription units
    Antony Rodriguez
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, United Kingdom
    Genome Res 14:1902-10. 2004
    ....
  41. pmc QuantPrime--a flexible tool for reliable high-throughput primer design for quantitative PCR
    Samuel Arvidsson
    Max Planck Institute of Molecular Plant Physiology, Potsdam Golm, Germany
    BMC Bioinformatics 9:465. 2008
    ..Using currently available primer design tools, several interactive decisions have to be made, resulting in lengthy design processes with varying qualities of the assays...
  42. pmc Efficient target-selected mutagenesis in zebrafish
    Erno Wienholds
    Hubrecht Laboratory, The Netherlands Institute for Developmental Biology, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands
    Genome Res 13:2700-7. 2003
    ..Furthermore,we show that TILLING can be used to detect the full spectrum of ENU-induced mutations in a vertebrate genome with the presence of many naturally occurring polymorphisms...
  43. pmc CODEHOP (COnsensus-DEgenerate Hybrid Oligonucleotide Primer) PCR primer design
    Timothy M Rose
    Department of Pathobiology, School of Public Health and Community Medicine, University of Washington, Seattle, WA 98195, USA
    Nucleic Acids Res 31:3763-6. 2003
    ..The CODEHOP designer (http://blocks.fhcrc.org/codehop.html) is linked to BlockMaker and the Multiple Alignment Processor within the Blocks Database World Wide Web (http://blocks.fhcrc.org)...
  44. ncbi RF cloning: a restriction-free method for inserting target genes into plasmids
    Fusinita van den Ent
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK
    J Biochem Biophys Methods 67:67-74. 2006
    ..The absence of any alterations to the protein as well as the simplicity of both the primer design and the procedure itself makes it suitable for high-throughput expression and ideal for structural genomics...
  45. pmc A universal DNA mini-barcode for biodiversity analysis
    Isabelle Meusnier
    Biodiversity Institute of Ontario, University of Guelph, Guelph, Ontario, N1G 2W1, Canada
    BMC Genomics 9:214. 2008
    ..It may be difficult in practice, however, to retrieve a 650 bp fragment from archival specimens, (because of DNA degradation) or from environmental samples (where universal primers are needed)...
  46. pmc Telomere measurement by quantitative PCR
    Richard M Cawthon
    Department of Human Genetics, University of Utah, 15 N 2030 E, Room 2100, Salt Lake City, UT 84112, USA
    Nucleic Acids Res 30:e47. 2002
    ..This assay will facilitate investigations of the biology of telomeres and the roles they play in the molecular pathophysiology of diseases and aging...
  47. pmc Disruption of the Cbfa2 gene causes necrosis and hemorrhaging in the central nervous system and blocks definitive hematopoiesis
    Q Wang
    Department of Biochemistry, Dartmouth Medical School, Hanover, NH 03755, USA
    Proc Natl Acad Sci U S A 93:3444-9. 1996
    ..Definitive erythropoiesis and myelopoiesis do not occur in Cbfa2-deficient embryos, and disruption of one copy of the Cbfa2 gene significantly reduces the number of progenitors for erythroid and myeloid cells...
  48. pmc Development of a real-time PCR assay for detection of Plasmodium falciparum, Plasmodium vivax, and Plasmodium ovale for routine clinical diagnosis
    F Perandin
    Department of Laboratory Diagnosis, Section of Microbiology, University of Brescia, Spedali Civili, 25123 Brescia, Italy
    J Clin Microbiol 42:1214-9. 2004
    ..The assay can therefore be easily implemented in routine diagnostic malaria tests. Future studies are warranted to investigate the clinical value of this technique...
  49. ncbi Web-based primer design for single nucleotide polymorphism analysis
    Michael M Neff
    Dept of Biology, Washington University, Campus Box 1137, St Louis, MO 63130 4899, USA
    Trends Genet 18:613-5. 2002
    ..Here we describe a web-based program, dCAPS Finder 2.0, that facilitates the design of mismatched PCR primers to create or remove a restriction endonuclease recognition site relative to the polymorphism being analyzed...
  50. ncbi A quantitative assay for HIV DNA integration in vivo
    S L Butler
    Infectious Disease Laboratory, The Salk Institute for Biological Studies, La Jolla, California, USA
    Nat Med 7:631-4. 2001
    ..The integration assay employs a novel quantitative form of Alu-PCR that should be generally applicable to studies of integrating viruses and gene transfer vectors...
  51. pmc Towards high-throughput molecular detection of Plasmodium: new approaches and molecular markers
    Nicolas Steenkeste
    Institut Pasteur du Cambodge, Laboratoire d Epidemiologie Moleculaire, Phnom Penh, Cambodia
    Malar J 8:86. 2009
    ..But sensitive diagnosis tools enabling to deal with a large number of samples are needed. The molecular detection approach offers a much higher sensitivity, and the flexibility to be automated and upgraded...
  52. ncbi Tumor spectrum analysis in p53-mutant mice
    T Jacks
    Center for Cancer Research, Massachusetts Institute of Technology, Cambridge 02139
    Curr Biol 4:1-7. 1994
    ..The construction of mouse strains carrying germline mutations of p53 facilitates analysis of the function of p53 in normal cells and tumorigenesis...
  53. pmc Bisulfite genomic sequencing: systematic investigation of critical experimental parameters
    C Grunau
    Institute of Molecular Biotechnology, Department of Genome Analysis, Beutenbergstrasse 11, 07745 Jena, Germany
    Nucleic Acids Res 29:E65-5. 2001
    ..The recognition rates for cytosine (>/=97%) and 5-methylcytosine (>/=94%) were found to be identical for both templates...
  54. pmc FIH-1: a novel protein that interacts with HIF-1alpha and VHL to mediate repression of HIF-1 transcriptional activity
    P C Mahon
    Institute of Genetic Medicine, Departments of Pediatrics and Medicine, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21287 3914, USA
    Genes Dev 15:2675-86. 2001
    ..Involvement of VHL in association with FIH-1 provides a unifying mechanism for the modulation of HIF-1alpha protein stabilization and transcriptional activation in response to changes in cellular O(2) concentration...
  55. pmc Applied genomics: data mining reveals species-specific malaria diagnostic targets more sensitive than 18S rRNA
    Allison Demas
    Malaria Branch, Division of Parasitic Diseases and Malaria, Center for Global Health, Centers for Disease Control and Prevention, Atlanta, Georgia 30602, USA
    J Clin Microbiol 49:2411-8. 2011
    ..These novel targets provide a powerful alternative molecular diagnostic method for the detection of P. falciparum and P. vivax in conventional or multiplex PCR platforms...
  56. ncbi Double-joint PCR: a PCR-based molecular tool for gene manipulations in filamentous fungi
    Jae Hyuk Yu
    Department of Food Microbiology and Toxicology, The University of Wisconsin, Madison, WI 53706, USA
    Fungal Genet Biol 41:973-81. 2004
    ..To stream line the analysis of the transformants a relatively simple procedure for genomic DNA or total RNA isolation achieving approximately 100 samples/person/day is also presented...
  57. pmc A soybean transcript map: gene distribution, haplotype and single-nucleotide polymorphism analysis
    Ik Young Choi
    Soybean Genomics and Improvement Laboratory, USDA, ARS, Beltsville, Maryland 20705, USA
    Genetics 176:685-96. 2007
    ....
  58. pmc ITS as an environmental DNA barcode for fungi: an in silico approach reveals potential PCR biases
    Eva Bellemain
    National Centre for Biosystematics, University of Oslo, Norway
    BMC Microbiol 10:189. 2010
    ..We performed in silico PCR analyses with commonly used primer combinations using various ITS datasets obtained from public databases as templates...
  59. ncbi The Vif protein of HIV triggers degradation of the human antiretroviral DNA deaminase APOBEC3G
    Silvestro G Conticello
    Medical Research Council, Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, United Kingdom
    Curr Biol 13:2009-13. 2003
    ..Inhibitors of this interaction might therefore prove therapeutically useful in blocking Vif-mediated APOBEC3G destruction...
  60. pmc Seed-specific over-expression of an Arabidopsis cDNA encoding a diacylglycerol acyltransferase enhances seed oil content and seed weight
    C Jako
    Seed Oil Biotechnology Group, National Research Council of Canada, Plant Biotechnology Institute, 110 Gymnasium Place, Saskatoon, Saskatchewan S7N 0W9, Canada
    Plant Physiol 126:861-74. 2001
    ..The DGAT activity in developing seed of transgenic lines was enhanced by 10% to 70%. Thus, the current study confirms the important role of DGAT in regulating the quantity of seed triacylglycerols and the sink size in developing seeds...
  61. pmc High-throughput stem-loop RT-qPCR miRNA expression profiling using minute amounts of input RNA
    Pieter Mestdagh
    Center for Medical Genetics, Ghent University Hospital, Ghent, Belgium
    Nucleic Acids Res 36:e143. 2008
    ..The high specificity of RT-qPCR together with a superior sensitivity makes this approach the method of choice for high-throughput miRNA expression profiling...
  62. ncbi Large deletions within the first intron in VRN-1 are associated with spring growth habit in barley and wheat
    Daolin Fu
    Department of Plant Sciences, University of California, One Shields Av, Davis, CA 95616, USA
    Mol Genet Genomics 273:54-65. 2005
    ..Epistatic interactions between VRN-H2 and the VRN-H1 allele with the intron 1 deletion suggest that the deleted region may include a recognition site for the flowering repression mediated by the product of the VRN-H2 gene of barley...
  63. ncbi Maternal wnt11 activates the canonical wnt signaling pathway required for axis formation in Xenopus embryos
    Qinghua Tao
    Division of Developmental Biology, Cincinnati Children s Research Foundation, 3333 Burnet Avenue, Cincinnati, Ohio 45229, USA
    Cell 120:857-71. 2005
    ..Second, we show that activation requires the glycosyl transferase X.EXT1. Third, we find that the EGF-CFC protein, FRL1, is also essential and interacts with Wnt11 to activate canonical Wnt signaling...
  64. pmc Specific and sensitive quantitative RT-PCR of miRNAs with DNA primers
    Ingrid Balcells
    Departament de Ciencia Animal i dels Aliments, Universitat Autonoma de Barcelona, 08193 Bellaterra, Spain
    BMC Biotechnol 11:70. 2011
    ..Due to the important biological role it is of great interest to quantitatively determine their expression level in different biological settings...
  65. ncbi Zebrafish Dkk1 functions in forebrain specification and axial mesendoderm formation
    H Hashimoto
    Division of Molecular Oncology, Biomedical Research Center, Osaka University Graduate School of Medicine, 2 2 Yamada oka, Suita, Osaka, 565 0871, Japan
    Dev Biol 217:138-52. 2000
    ..These data indicate that Dkk1, expressed in dorsal mesendoderm, functions in the formation of both the anterior nervous system and the axial mesendoderm in zebrafish...
  66. pmc BIPES, a cost-effective high-throughput method for assessing microbial diversity
    Hong Wei Zhou
    Department of Environmental Health, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou, Guangdong, China
    ISME J 5:741-9. 2011
    ..As a laborsaving and cost-effective method, BIPES can be routinely used to analyze the microbial ecology of both environmental and human microbiomes...
  67. ncbi Effect of primer mismatch, annealing temperature and PCR cycle number on 16S rRNA gene-targetting bacterial community analysis
    Rita Sipos
    Department of Microbiology, Eötvös Loránd University of Science, Budapest, Hungary
    FEMS Microbiol Ecol 60:341-50. 2007
    ..As a result of additional tests on environmental samples, the use of a low annealing temperature is recommended in order to significantly reduce preferential amplification while maintaining the specificity of PCR...
  68. ncbi Defects of B-cell lymphopoiesis and bone-marrow myelopoiesis in mice lacking the CXC chemokine PBSF/SDF-1
    T Nagasawa
    Department of Immunology, Research Institute, Osaka Medical Center for Maternal and Child Health, Japan
    Nature 382:635-8. 1996
    ..In addition, the mutants had a cardiac ventricular septal defect. Hence, we have shown that the chemokine PBSF/SDF-1 has several essential functions in development...
  69. pmc RTPrimerDB: the portal for real-time PCR primers and probes
    Steve Lefever
    Center for Medical Genetics, Ghent University Hospital, De Pintelaan 185, 9000 Gent, Belgium
    Nucleic Acids Res 37:D942-5. 2009
    ..RTPrimerDB is a one-stop portal for high-quality and highly annotated real-time PCR assays...
  70. pmc PCR as a confirmatory technique for laboratory diagnosis of malaria
    Stephanie P Johnston
    Division of Parasitic Diseases, M S F 36, Centers for Disease Control and Prevention, U S Department of Health and Human Services, 4770 Buford Highway NE, Atlanta, Georgia 30341 3724, USA
    J Clin Microbiol 44:1087-9. 2006
    ..in blood specimens. PCR was more sensitive than microscopy and capable of identifying malaria parasites at the species level when microscopy was equivocal...
  71. pmc Plasticity in the organization and sequences of human KIR/ILT gene families
    M J Wilson
    Immunology Division, Department of Pathology, Tennis Court Road, Cambridge CB2 1QP, United Kingdom
    Proc Natl Acad Sci U S A 97:4778-83. 2000
    ..KIR genesis was followed by extensive duplication/deletion as well as intergenic sequence exchange, reminiscent of MHC class I genes, which provide KIR ligands...
  72. ncbi Polymerase chain reaction primers miss half of rRNA microbial diversity
    Sunhee Hong
    Department of Biology, Northeastern University, Boston, MA, USA
    ISME J 3:1365-73. 2009
    ....
  73. ncbi Stem-cell protein Piwil2 is widely expressed in tumors and inhibits apoptosis through activation of Stat3/Bcl-XL pathway
    Jae Ho Lee
    Institute of Human Genetics, University of Gottingen, 37073 Gottingen, Germany
    Hum Mol Genet 15:201-11. 2006
    ..Expression of Piwil2 in a wide variety of tumors could be a useful prognostic factor that could have also diagnostic and therapeutic implications...
  74. pmc Bone marrow CD169+ macrophages promote the retention of hematopoietic stem and progenitor cells in the mesenchymal stem cell niche
    Andrew Chow
    Department of Gene and Cell Medicine, Mount Sinai School of Medicine, New York, NY 10029, USA
    J Exp Med 208:261-71. 2011
    ..Thus, strategies that target BM MΦ hold the potential to augment stem cell yields in patients that mobilize HSCs/progenitors poorly...
  75. ncbi Validation of housekeeping genes for gene expression studies in Symbiodinium exposed to thermal and light stress
    Nedeljka N Rosic
    Global Change Institute, University of Queensland, St Lucia, Brisbane, 4072, QLD, Australia
    Mar Biotechnol (NY) 13:355-65. 2011
    ..This study provides the first list of the HKGs and will provide a useful reference in future gene expression studies in symbiotic dinoflagellates...
  76. ncbi Uncultured archaea in deep marine subsurface sediments: have we caught them all?
    Andreas Teske
    Department of Marine Sciences, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    ISME J 2:3-18. 2008
    ....
  77. pmc Detection of influenza A viruses from different species by PCR amplification of conserved sequences in the matrix gene
    R A Fouchier
    National Influenza Center and Department of Virology, Erasmus University, Rotterdam, The Netherlands
    J Clin Microbiol 38:4096-101. 2000
    ..Comparative tests with throat swab samples from humans and fecal and cloacal swab samples from birds confirmed that the new PCR is faster and up to 100-fold more sensitive than classical virus isolation procedures...
  78. ncbi The head involution defective gene of Drosophila melanogaster functions in programmed cell death
    M E Grether
    Howard Hughes Medical Institute, Department of Brain and Cognitive Sciences, Cambridge, Massachusetts, USA
    Genes Dev 9:1694-708. 1995
    ..This phenotype can be suppressed completely by expression of the anti-apoptotic p35 protein from baculovirus, indicating that p35 may act genetically downstream from hid...
  79. ncbi Regulation of Gli2 and Gli3 activities by an amino-terminal repression domain: implication of Gli2 and Gli3 as primary mediators of Shh signaling
    H Sasaki
    Laboratory of Developmental Biology, Institute for Molecular and Cellular Biology, Osaka University, Suita, Osaka 565 0871, Japan
    Development 126:3915-24. 1999
    ....
  80. ncbi Analysis of Australian fur seal diet by pyrosequencing prey DNA in faeces
    Bruce E Deagle
    Australian Marine Mammal Centre, Australian Antarctic Division, Tasmania, Australia
    Mol Ecol 18:2022-38. 2009
    ..The pyrosequencing approach presented significantly expands the capabilities of DNA-based methods of dietary analysis and is suitable for large-scale diet investigations on a broad range of animals...
  81. pmc BARCRAWL and BARTAB: software tools for the design and implementation of barcoded primers for highly multiplexed DNA sequencing
    Daniel N Frank
    Department of Molecular, University of Colorado, Boulder, CO 80309, USA
    BMC Bioinformatics 10:362. 2009
    ....
  82. ncbi Mapping of picoeucaryotes in marine ecosystems with quantitative PCR of the 18S rRNA gene
    Fei Zhu
    Station Biologique, UMR 7127 CNRS, INSU et Université Pierre et Marie Curie, Place George Teissier, 29680 Roscoff, France
    FEMS Microbiol Ecol 52:79-92. 2005
    ..Data interpretation must take into account primer specificity and the varying rRNA gene copy number among eukaryotes...
  83. pmc Leptomycin B inactivates CRM1/exportin 1 by covalent modification at a cysteine residue in the central conserved region
    N Kudo
    Department of Biotechnology, Graduate School of Agriculture and Life Sciences, The University of Tokyo, Bunkyo ku, Tokyo 113 8657, Japan
    Proc Natl Acad Sci U S A 96:9112-7. 1999
    ..These results show that the single cysteine residue determines LMB sensitivity and is selectively alkylated by LMB, leading to CRM1 inactivation...
  84. ncbi Strategies for microsatellite isolation: a review
    L Zane
    Dipartimento di Biologia, Università di Padova Via Ugo Bassi 58 B, 35121, Padova, Italy
    Mol Ecol 11:1-16. 2002
    ..In addition, we propose a fast and easy protocol which is a combination of different published methods...
  85. ncbi Abnormal blood vessel development and lethality in embryos lacking a single VEGF allele
    P Carmeliet
    Center for Transgene Technology and Gene Therapy, Flanders Interuniversity Institute for Biotechnology, Leuven, Belgium
    Nature 380:435-9. 1996
    ....
  86. pmc Hydrazine synthase, a unique phylomarker with which to study the presence and biodiversity of anammox bacteria
    Harry R Harhangi
    Department of Microbiology, Institute for Water and Wetland Research IWWR, Radboud University Nijmegen, Nijmegen, The Netherlands
    Appl Environ Microbiol 78:752-8. 2012
    ....
  87. pmc A method for studying protistan diversity using massively parallel sequencing of V9 hypervariable regions of small-subunit ribosomal RNA genes
    Linda A Amaral-Zettler
    The Josephine Bay Paul Center for Comparative Molecular Biology and Evolution, Marine Biological Laboratory, Woods Hole, MA, USA
    PLoS ONE 4:e6372. 2009
    ..Recent advances in pyrosequencing technology provide read lengths of up to 240 nucleotides. Amplicon pyrosequencing can now be applied to longer variable regions of the SSU rRNA gene including the V9 region in eukaryotes...
  88. pmc Quantitative analysis of diverse Lactobacillus species present in advanced dental caries
    Roy Byun
    Institute of Dental Research, Westmead Centre for Oral Health, P O Box 533, Wentworthville, NSW 2145, Australia
    J Clin Microbiol 42:3128-36. 2004
    ..gasseri and L. ultunensis than of the other prevalent species. The findings provide a basis for further characterization of the pathogenicity of Lactobacillus spp. in the context of extension of the carious lesion...
  89. ncbi A method for the absolute quantification of cDNA using real-time PCR
    Joseph A Whelan
    Onyvax Ltd, St George s Hospital Medical School, Cranmer Terrace, London SW17 0RE, UK
    J Immunol Methods 278:261-9. 2003
    ..Although our interest has been cytokine quantification, we believe that this technique is widely applicable to the majority of real-time PCR applications...
  90. pmc BatchPrimer3: a high throughput web application for PCR and sequencing primer design
    Frank M You
    Department of Plant Sciences, University of California, CA 95616, USA
    BMC Bioinformatics 9:253. 2008
    ..In particular, most programs lack batch primer design capability. Such a high-throughput software tool for designing SSR flanking primers and SNP genotyping primers is increasingly demanded...
  91. ncbi Tumor-associated endothelial cells with cytogenetic abnormalities
    Kyoko Hida
    Vascular Biology Program, Department of Surgery, Children s Hospital and Harvard Medical School, Boston, Massachusetts 02115, USA
    Cancer Res 64:8249-55. 2004
    ..FISH analysis of tumor sections also showed endothelial cell aneuploidy. We conclude that tumor endothelial cells can acquire cytogenetic abnormalities while in the tumor microenvironment...
  92. pmc Osteoclast differentiation independent of the TRANCE-RANK-TRAF6 axis
    Nacksung Kim
    Medical Research Center for Gene Regulation, Chonnam National University Medical School, Gwangju, Korea
    J Exp Med 202:589-95. 2005
    ..We provide direct evidence against the current paradigm that the TRANCE-RANK-TRAF6 pathway is essential for osteoclast differentiation and suggest the potential existence of alternative routes for osteoclast differentiation...
  93. pmc Microsatellites as DNA markers in cultivated peanut (Arachis hypogaea L.)
    Guohao He
    Tuskegee University, Tuskegee, AL 36088, USA
    BMC Plant Biol 3:3. 2003
    ..Genomic research of cultivated peanut has lagged behind other crop species because of the paucity of polymorphic DNA markers found in this crop. It is necessary to identify additional DNA markers for further genetic research in peanut...
  94. ncbi In silico analysis on frequency and distribution of microsatellites in ESTs of some cereal species
    Rajeev K Varshney
    Institute of Plant Genetics and Crop Plant Research IPK, Corrensstrasse 3, D 06466 Gatersleben, Germany
    Cell Mol Biol Lett 7:537-46. 2002
    ....
  95. pmc Capsular polysaccharide synthesis regions in Klebsiella pneumoniae serotype K57 and a new capsular serotype
    Yi Jiun Pan
    Department of Microbiology, National Taiwan University College of Medicine, Taipei, Taiwan
    J Clin Microbiol 46:2231-40. 2008
    ..We conclude that sequences from these two cps regions are very useful in detecting K57 and the new cps genotype...
  96. ncbi Use of sequence data from rainbow trout and Atlantic salmon for SNP detection in Pacific salmon
    Christian T Smith
    Gene Conservation Laboratory, Division of Commercial Fisheries, Alaska Department of Fish and Game, 333 Raspberry Road, Anchorage, AK 99518, USA
    Mol Ecol 14:4193-203. 2005
    ..While the costs of DNA sequencing are still significant, these must be compared to the costs of using other marker classes for a given application...
  97. pmc LINE-1 hypomethylation in cancer is highly variable and inversely correlated with microsatellite instability
    Marcos R H Estecio
    Department of Leukemia, University of Texas M D Anderson Cancer Center, Houston, Texas, United States of America
    PLoS ONE 2:e399. 2007
    ..This study was performed to determine the relationship between DNA hypomethylation, hypermethylation and microsatellite instability in cancer...
  98. pmc The use of coded PCR primers enables high-throughput sequencing of multiple homolog amplification products by 454 parallel sequencing
    Jonas Binladen
    Center for Ancient Genetics, Institute of Biology, University of Copenhagen, Copenhagen, Denmark
    PLoS ONE 2:e197. 2007
    ....
  99. pmc Characterization of Dicer-deficient murine embryonic stem cells
    Elizabeth P Murchison
    Watson School of Biological Sciences, Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, NY 11724, USA
    Proc Natl Acad Sci U S A 102:12135-40. 2005
    ..These studies provide a conditional model of RNAi deficiency in mammals that will permit the dissection of the biological roles of the RNAi machinery in cultured mammalian cells...
  100. ncbi Robustness of a loop-mediated isothermal amplification reaction for diagnostic applications
    Patrice Francois
    Genomic Research Laboratory, University Hospitals of Geneva, Service of Infectious Diseases, Geneva, Switzerland
    FEMS Immunol Med Microbiol 62:41-8. 2011
    ..Our results demonstrate that LAMP is not only easy to use, but is also a very robust, innovative and powerful molecular diagnostic method for both industrialized and developing countries...
  101. ncbi Cost-effective, species-specific microsatellite development for the endangered Dwarf Bulrush (Typha minima) using next-generation sequencing technology
    Daniela Csencsics
    Research Unit Ecological Genetics and Evolution, Swiss Federal Research Institute WSL, Zürcherstrasse 111, CH 8903 Birmensdorf, Switzerland
    J Hered 101:789-93. 2010
    ....

Research Grants53

  1. Amplifying mRNA Sequences from Small Amounts of Tissue
    Paul Siebert; Fiscal Year: 2006
    ..In the Phase I Research we propose to develop such a method, and utilize a set on non-random DNA primers for the synthesis and amplification of cDNA...
  2. Providing the $1000 genome via improved single molecule sequencing
    JOHN FENTON THOMPSON; Fiscal Year: 2010
    ..By developing an ordered surface of DNA primers, up to 5x increase in sequencing yield is possible...
  3. DNA sequencing by ELFSE on microfluidic devices
    Annelise Barron; Fiscal Year: 2006
    ..Optimized protocols for conjugation of drag-tags to DNA primers, cycle sequencing, and sample cleanup will be developed...
  4. ADDRESSING THE ISSUE OF GAP REGIONS IN GENOME SEQUENCING
    John SantaLucia; Fiscal Year: 2001
    ..The Watson-Crick pairing rules are not sufficient to design robust DNA primers or hybridization arrays...
  5. Novel Real Time Isothermal DNA Amplification/Detection
    WILLIAM MACCONNELL; Fiscal Year: 2005
    ..the "hairpin loop concatamer DNA amplification" (HLCA) process which incorporates self-priming hairpin oligo DNA primers into linear DNA during the initial rounds of replication (5'8)...
  6. POST-TRANSCRIPTIONAL MODIFICATIONS IN RIBOSOMAL RNA
    David Jemiolo; Fiscal Year: 1990
    ..Modifications will be located by sequencing rRNA using DNA primers and reverse transcriptase...
  7. DNA BASE CALLING USING HETERODYNE FLUORESCENCE DETECTION
    Kristen Peterson; Fiscal Year: 1999
    ..The Phase I project will demonstrate our approach by identifying and distinguishing DNA primers tagged with recently developed dyes that absorb red light and have lifetimes of l to 5 nsecs...
  8. Novel Real Time Isothermal DNA Amplification/Detection
    WILLIAM MACCONNELL; Fiscal Year: 2003
    ..the "hairpin loop concatamer DNA amplification" (HLCA) process, which incorporates self-priming hairpin oligo DNA primers into linear DNA during the initial rounds of replication...
  9. INT-1 PROTO-ONCOGENE FAMILY
    GREGORY SHACKLEFORD; Fiscal Year: 1993
    ..describes: 1) The polymerase chain reaction will be used to amplify DNA from genes in the int-1 family using DNA primers corresponding to conserved regions in the 3 presently known members...
  10. Multiplexed Reactive Sequencing of DNA
    Peter Williams; Fiscal Year: 2007
    DESCRIPTION (provided by applicant): A new approach to DNA sequencing is proposed. DNA primers synthesized to be complementary to specific sequences on targeted genes will be covalently tethered in known locations in an array on the ..
  11. In vitro culture and transformation of Wolbachia from mosquitoes
    Ann Fallon; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  12. Prognostic Significance of DNA and Histone Methylation
    Chandrika Piyathilake; Fiscal Year: 2007
    ..Investigator talent, equipment, facilities and access to study subjects at UAB are ideal for conducting this study. ..
  13. Ethanol Effects on Human Astroglial Chemokine Expression
    Randall Davis; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  14. GBS: Epidemiologic Characterization for Gene Discovery
    Betsy Foxman; Fiscal Year: 2007
    ..A better understanding of the molecular epidemiology and the identification of new GBS virulence genes will facilitate the discover of new therapies and prevention strategies for GBS disease. ..
  15. HU-UPCI Cancer Education/Career Development Partnership
    Richard Steinman; Fiscal Year: 2006
    ..abstract_text> ..
  16. Mechanisms and Functions of Human Sulfotransferases
    Guangping Chen; Fiscal Year: 2009
    ..The knowledge will be important in understanding drug side effect, drug-drug interaction, drug development, and the potential roles SULTs play in cancer prevention and causation. ..
  17. Oral and Vaginal Microbes, Human Genotype, and Preterm Birth
    Dawn P Misra; Fiscal Year: 2010
    ..For this proposal we have the great advantage of exploring these hypotheses using already collected specimens and data as part of completed studies of preterm birth. ..
  18. Muscle protein synthesis and gene expression in myostatin-deficient mice
    Stephen L Welle; Fiscal Year: 2010
    ..Knowledge of the physiological and molecular effects of reducing myostatin activity in mature muscle will reveal potential benefits and limitations of anti-myostatin therapy. ..
  19. HPV Clearance by Folic Acid Supplementation
    Chandrika J Piyathilake; Fiscal Year: 2010
    ..Because this study will be conducted among women positive for HPV 16, the most prevalent and powerful causative agent of CC, the public health significance of this study could be enormous and will complement HPV vaccine studies. ..
  20. Safety of Nicotine Reduction Strategy
    Neal Benowitz; Fiscal Year: 2009
    ..The proposed research is needed to guide governmental regulators as to how best to regulate nicotine in cigarettes and is an essential first step in examining the feasibility of a national regulation program. ..
  21. Redox-sensitive developmental pathways and gene regulatory networks
    JAMES COFFMAN; Fiscal Year: 2009
    ..Finally, the Comparative Toxicogenomics Database (CTD) at MDIBL will be used to determine the relevance of the pathways discovered in sea urchins to human health, and to generate hypotheses that might explain specific human diseases. ..
  22. Control of Cell Proliferation by Runx Proteins
    JAMES COFFMAN; Fiscal Year: 2009
    ..purpuratus genome sequence, which will greatly facilitate both the cis-regulatory analysis of genes and the identification of purified proteins by mass spectrometry. ..
  23. Database of Functional SNPs in Cancer-Related Environmentally Responsive Genes
    Yong Zhu; Fiscal Year: 2009
    ..Findings from this study will enhance the use of SNPs in future phenotypic studies and molecular epidemiologic approaches to address questions related to gene-environment interaction in disease risk estimate. ..
  24. The Role of Mitochondrial DNA Alterations in Cancer
    Lee Jun Wong; Fiscal Year: 2008
    ..Results from this research project will help us understand the functional role of mitochondrial DNA alterations in cancer and identify potential novel targets for more effective therapeutic development. ..
  25. HUMAN OXIDATION RESISTANCE GENES
    Michael Volkert; Fiscal Year: 2007
    ..abstract_text> ..
  26. Role of Retinal Microvasculature in Posterior Uveitis
    Justine Smith; Fiscal Year: 2006
    ..The studies should also have implications for unrelated diseases affecting the retinal circulation, as well as tissue-specific inflammations and infections at other body sites. [unreadable] [unreadable]..
  27. NEUROTROPIC CYTOMEGALOVIRUS DURING IMMUNOSUPPRESSION
    JON REUTER; Fiscal Year: 2006
    ..abstract_text> ..
  28. NUCLEOTIDE DONORS AFFECTING GLYCOSYLATION PATHWAYS
    Ajit Varki; Fiscal Year: 2003
    ....
  29. ACTIVE SITE STUDIES OF HUMAN SULFOTRANSFERASES
    Guangping Chen; Fiscal Year: 2003
    ..The characterization of the active sites of STs will also help to find mechanism-based specific inhibitors/activators for use in studying the physiological functions of STs and designing new drugs. ..
  30. CHANGES IN GENE EXPRESSION IN SENESCENT MUSCLE
    Stephen Welle; Fiscal Year: 2003
    ..abstract_text> ..
  31. Ethanol and NOS2 Gene Expression in Human Astrocytes
    Randall Davis; Fiscal Year: 2004
    ..Use of deletion constructs will help identify ethanol-sensitive regions for future studies on trans-acting factors involved in the action of ethanol. ..
  32. SYNTHESIS AND DEGRADATION OF RIBOSOMES IN THE MOSQUITO
    Ann Fallon; Fiscal Year: 2004
    ..These studies provide cloned genes, molecular approaches, and characterization of regulatory processes that can potentially be manipulated to interfere with disease transmission by blood-feeding arthropods. ..
  33. MOSQUITO IMMUNITY FUNCTIONS
    Ann Fallon; Fiscal Year: 2003
    ..The work will include identification of exon-intron organization and recovery of upstream flanking DNA, which will be analyzed for potential regulatory elements. ..
  34. FUNCTION AND REGULATION OF THE E COLI AIDB GENES
    Michael Volkert; Fiscal Year: 2002
    ..During the proposed funding period, I propose to characterize this factor further by genetic and biochemical means and to compare its induction mechanism with that of meAda. ..
  35. Prognostic Significance of Altered DNA Methylation
    Chandrika Piyathilake; Fiscal Year: 2002
    ..A newly developed and tested immunohistochemical technique, which measures the degree of global methylation in intact and specific types of cells, will be used to evaluate global DNA methylation in the proposed study. ..
  36. Effects of Folate Fortification on Cancer Prevention an*
    Chandrika Piyathilake; Fiscal Year: 2005
    ....
  37. ECDYSONE EFFECTS ON MOSQUITO CELL PROLIFERATION
    Ann Fallon; Fiscal Year: 2001
    ..Results of this study will provide novel insights into the effects of 20E on cell growth and proliferation, which are directly relevant to efforts to control vector-borne disease by genetic manipulation of insect vectors. ..
  38. CHEMOKINES/CYTOKINES AND RECEPTORS IN STEM CELL HOMING
    Hal Broxmeyer; Fiscal Year: 2005
    ..These studies should clarity the relevance of SDF-1 and CXCR4 for homing/engraftment and mobilization of primary stem/progenitor cells. ..
  39. MOLECULAR CONTRIBUTORS TO STEM CELL QUIESCENCE
    Richard Steinman; Fiscal Year: 2003
    ..Insights gained from these studies should have a significant impact on gene therapy strategies and should generate novel tools for the study of leukemias and myelodysplastic syndromes. ..
  40. A Nucleosomal Biosensor for Identification and Isolation of Nuclear Hormone Recep
    Richard Steinman; Fiscal Year: 2008
    ..This biosensor should be more rapid and versatile than cell-based reporter gene assays and more informative than assays that measure only ligand/receptor binding. [unreadable] [unreadable] [unreadable]..
  41. MGB Eclipse Probe Detection of Category A Organisms
    Irina Afonina; Fiscal Year: 2003
    ..In Phase II, we will expand the number of Category A organisms and improve throughput by multiplexing. In addition, commercial prototype MGB Eclipse(TM) probe assays will be developed for each of these organisms. ..
  42. Attenuation of astroglial chemokine expression by beta-funaltrexamine: implicatio
    Randall Davis; Fiscal Year: 2008
    ..This training will lend to the success of these students in their future careers in health related professions. [unreadable] [unreadable] [unreadable]..
  43. Chemokines in host defense to Campylobacter jejuni
    MICHAEL DWINELL; Fiscal Year: 2004
    ..jejuni infection are central to the development of preventative therapeutic strategies to modulate host-pathogen interactions to favor the host. ..
  44. Hypoxia and the Role of HIF-1 in the Ischemic Testis
    Michael Palladino; Fiscal Year: 2005
    ..abstract_text> ..
  45. Mobilization of Candida albicans Biofilms
    Peter Suci; Fiscal Year: 2007
    ..albicans biofilm communities, it will provide the basis for development of methods for screening for adjuvant agents that will inhibit dissemination of these fungal infections. [unreadable] [unreadable] [unreadable]..
  46. Inhibition of NF-KB to Facilitate Islet Transplantation
    Paul Robbins; Fiscal Year: 2003
    ..The successful completion of the proposed studies should determine if NF-kB inhibition in islets by peptide and gene mediated transduction is able to improve islet viability prior to and post-transplantation. ..
  47. REGULATION OF TRANSCRIPTION BY THE RB ANTIONCOGENE
    Paul Robbins; Fiscal Year: 2002
    ..The experiments described in the proposal are expected to provide important insights into the mechanism of how RB and cyclin D1 function to regulate transcription directly, cell differentiation, and tumorigenesis. ..
  48. Improved Brucella Vaccine Strains
    Thomas Ficht; Fiscal Year: 2009
    ..abstract_text> ..
  49. 2008 Viral Vectors for Gene Therapy, The Science of Gordon Research Conference
    Paul Robbins; Fiscal Year: 2008
    ..End of Abstract) [unreadable] [unreadable] [unreadable]..
  50. Mechanisms of Regulation of GLUT4 Expression
    ANN OLSON; Fiscal Year: 2005
    ..abstract_text> ..
  51. Neurogenesis as a compensatory mechanism to regulate energy balance
    ALLISON XU; Fiscal Year: 2008
    ..The identification of neuronal substrates critical for body weight regulation will lay the groundwork for future therapeutic interventions designed to target these specific neurons. [unreadable] [unreadable] [unreadable]..
  52. Insulin Regulation of Adipocyte Microtubules
    ANN OLSON; Fiscal Year: 2008
    ..unreadable] [unreadable]..
  53. Dendritic Cells For Prevention of Islet Graft Rejection
    Karl Womer; Fiscal Year: 2008
    ..Finally, our studies will be important to the development of clinical strategies designed to prolong the survival of islet cell transplants. ..