Summary: Short sequences (generally about 10 base pairs) of DNA that are complementary to sequences of messenger RNA and allow reverse transcriptases to start copying the adjacent sequences of mRNA. Primers are used extensively in genetic and molecular biology techniques.
Publications275 found, 100 shown here
- Primer3 on the WWW for general users and for biologist programmersS Rozen
Whitehead Institute for Biomedical Research, Cambridge, MA, USA
Methods Mol Biol 132:365-86. 2000
- A new mathematical model for relative quantification in real-time RT-PCRM W Pfaffl
Institute of Physiology, FML Weihenstephan, Center of Life and Food Sciences, Technical University of Munich, Germany
Nucleic Acids Res 29:e45. 2001..High accuracy and reproducibility (<2.5% variation) were reached in LightCycler PCR using the established mathematical model...
- DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebratesO Folmer
Center for Theoretical and Applied Genetics, Rutgers University, New Brunswick, New Jersey 08903 231
Mol Mar Biol Biotechnol 3:294-9. 1994We describe "universal" DNA primers for polymerase chain reaction (PCR) amplification of a 710-bp fragment of the mitochondrial cytochrome c oxidase subunit I gene (COI) from 11 invertebrate phyla: Echinodermata, Mollusca, Annelida, ..
- Primer3Plus, an enhanced web interface to Primer3Andreas Untergasser
Laboratory of Molecular Biology, Department of Plant Science, Wageningen University, Dreijenlaan 3, 6703HA, Wageningen, The Netherlands
Nucleic Acids Res 35:W71-4. 2007..The Primer3Plus Perl source code is available under GPL license from SourceForge. Primer3Plus is available at http://www.bioinformatics.nl/primer3plus...
- Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assaysS A Bustin
Academic Department of Surgery, St Bartholomew s and the Royal London School of Medicine and Dentistry, Queen Mary and Westfield College, London E1 1BB, UK
J Mol Endocrinol 25:169-93. 2000..It illustrates the usefulness of these assays by demonstrating the significantly different levels of transcription between individuals of the housekeeping gene family, glyceraldehyde-3-phosphate-dehydrogenase (GAPDH)...
- MethPrimer: designing primers for methylation PCRsLong Cheng Li
Department of Urology, Veterans Affairs Medical Center, and University of California San Francisco, San Francisco, CA 94121, USA
Bioinformatics 18:1427-31. 2002..Therefore, the present study was designed to develop a program for such applications...
- Ten species in one: DNA barcoding reveals cryptic species in the neotropical skipper butterfly Astraptes fulgeratorPaul D N Hebert
Department of Zoology, University of Guelph, Guelph, ON, N1G 2W1, Canada
Proc Natl Acad Sci U S A 101:14812-7. 2004..They also illustrate the value of DNA barcoding, especially when coupled with traditional taxonomic tools, in disclosing hidden diversity...
- Guideline to reference gene selection for quantitative real-time PCRAleksandar Radonic
Charité Campus Charité Mitte, II Medizinische Klinik mit Schwerpunkt Onkologie und Hämatologie, Humboldt Universitat, Berlin, Germany
Biochem Biophys Res Commun 313:856-62. 2004..Our results show that "Classical" reference genes are indeed unsuitable, whereas the RNA polymerase II gene was the gene with the most constant expression in different tissues and following stimulation in CCRF-HSB-2 cells...
- Preservation of duplicate genes by complementary, degenerative mutationsA Force
Department of Biology, University of Oregon, Eugene, Oregon 97403, USA
Genetics 151:1531-45. 1999..Cooke et al. 1997 (p. 362)..
- An economic method for the fluorescent labeling of PCR fragmentsM Schuelke
Department of Neuropediatrics, Charite University Hospital, Augustenburger Platz 1, D 13353 Berlin, Germany
Nat Biotechnol 18:233-4. 2000..A poor man's approach to genotyping for research and high-throughput diagnostics...
- Real-time quantification of microRNAs by stem-loop RT-PCRCaifu Chen
Applied Biosystems, 850 Lincoln Centre Drive, Foster City, CA 94404, USA
Nucleic Acids Res 33:e179. 2005..Furthermore, the concept of stem-loop RT primer design could be applied in small RNA cloning and multiplex assays for better specificity and efficiency...
- An essential role for interferon gamma in resistance to Mycobacterium tuberculosis infectionJ L Flynn
Howard Hughes Medical Institute, Department of Microbiology, Albert Einstein College of Medicine, Bronx, New York 10461
J Exp Med 178:2249-54. 1993..In contrast to control mice, gko mice exhibit heightened tissue necrosis and succumb to a rapid and fatal course of tuberculosis that could be delayed, but not prevented, by treatment with exogenous recombinant IFN-gamma...
- Methylation-specific PCR: a novel PCR assay for methylation status of CpG islandsJ G Herman
Oncology Center, Johns Hopkins Medical Institutions, Baltimore, MD 21231, USA
Proc Natl Acad Sci U S A 93:9821-6. 1996....
- Design and standardization of PCR primers and protocols for detection of clonal immunoglobulin and T-cell receptor gene recombinations in suspect lymphoproliferations: report of the BIOMED-2 Concerted Action BMH4-CT98-3936J J M van Dongen
Department of Immunology, Erasmus MC, University Medical Center Rotterdam, Rotterdam, The Netherlands
Leukemia 17:2257-317. 2003..The BIOMED-2 multiplex tubes can now be used for diagnostic clonality studies as well as for the identification of PCR targets suitable for the detection of minimal residual disease...
- Review and re-analysis of domain-specific 16S primersG C Baker
Department of Biotechnology, University of the Western Cape, Bellville 7335, Cape Town, South Africa
J Microbiol Methods 55:541-55. 2003..These primers are found to amplify sequences from Crenarchaeote and Euryarchaeote type strains and environmental DNA...
- Conservative fragments in bacterial 16S rRNA genes and primer design for 16S ribosomal DNA amplicons in metagenomic studiesYong Wang
KAUST Global Partnership Program, Department of Biology, Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong, China
PLoS ONE 4:e7401. 2009..The primers recommended in this report shall facilitate a comprehensive and reliable survey of bacterial diversity in metagenomic studies...
- Light activation of channelrhodopsin-2 in excitable cells of Caenorhabditis elegans triggers rapid behavioral responsesGeorg Nagel
Max Planck Institute for Biophysics, Max von Laue Str 3, D 60438 Frankfurt, Germany
Curr Biol 15:2279-84. 2005..Thus, specific neurons or muscles expressing ChR2 can be quickly and reversibly activated by light in live and behaving, as well as dissected, animals...
- Short pyrosequencing reads suffice for accurate microbial community analysisZongzhi Liu
Department of Chemistry and Biochemistry, UCB 215, University of Colorado at Boulder, Boulder, CO 80309 0215, USA
Nucleic Acids Res 35:e120. 2007....
- Facile means for quantifying microRNA expression by real-time PCRRui Shi
North Carolina State University, Raleigh, NC 27695 7247, USA
Biotechniques 39:519-25. 2005..This method also revealed miRNA tissue-specific expression patterns that cannot be resolved by Northern blot analysis and may therefore be widely useful for characterizing miRNA expression in plants as well as in animals...
- Detection of loop-mediated isothermal amplification reaction by turbidity derived from magnesium pyrophosphate formationY Mori
Eiken Chemical Company, Ltd, 1381 3 Shimoishigami, Ohtawara, Tochigi 324 0036, Japan
Biochem Biophys Res Commun 289:150-4. 2001....
- PrimerBank: a resource of human and mouse PCR primer pairs for gene expression detection and quantificationAthanasia Spandidos
Department of Genetics, Harvard Medical School, Center for Computational and Integrative Biology, Massachusetts General Hospital, Boston, MA 02114 2790, USA
Nucleic Acids Res 38:D792-9. 2010..In all, 26,855 primer pairs covering most known mouse genes have been experimentally validated by QPCR, agarose gel analysis, sequencing and BLAST, and all validation data can be freely accessed from the PrimerBank web site...
- Improved amplification of genital human papillomavirusesP E Gravitt
Department of Human Genetics, Roche Molecular Systems, Alameda, California, USA
J Clin Microbiol 38:357-61. 2000..This new primer system will be useful in assessing the natural history of HPV infections, particularly when the analysis requires HPV typing...
- PerlPrimer: cross-platform, graphical primer design for standard, bisulphite and real-time PCROwen J Marshall
Murdoch Childrens Research Institute, Royal Childrens Hospital, Flemington Road, Parkville, Vic, 3052, Australia
Bioinformatics 20:2471-2. 2004..It aims to automate and simplify the process of primer design...
- An efficient one-step site-directed deletion, insertion, single and multiple-site plasmid mutagenesis protocolHuanting Liu
SSPF, Centre for Biomolecular Science, University of St Andrews, North Haugh, St Andrews KY16 9ST, UK
BMC Biotechnol 8:91. 2008..Although many site-directed mutagenesis methods have been developed, a simple, quick and multi-applicable method is still desirable...
- Dual roles for glucokinase in glucose homeostasis as determined by liver and pancreatic beta cell-specific gene knock-outs using Cre recombinaseC Postic
Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
J Biol Chem 274:305-15. 1999..Interestingly, hepatic GK knock-out mice also have impaired insulin secretion in response to glucose. These studies indicate that deficiencies in both beta cell and hepatic GK contribute to the hyperglycemia of MODY-2...
- Molecular beacons: probes that fluoresce upon hybridizationS Tyagi
Department of Molecular Genetics, Public Health Research Institute, New York, NY 10016, USA
Nat Biotechnol 14:303-8. 1996..When introduced into living cells, these probes should enable the origin, movement, and fate of specific mRNAs to be traced...
- Rapid amplification of plasmid and phage DNA using Phi 29 DNA polymerase and multiply-primed rolling circle amplificationF B Dean
Molecular Staging, Inc, New Haven, Connecticut 06511, USA
Genome Res 11:1095-9. 2001..Amplified products can also be used for in vitro cloning, library construction, and other molecular biology applications...
- The repertoire for pattern recognition of pathogens by the innate immune system is defined by cooperation between toll-like receptorsA Ozinsky
Department of Immunology, University of Washington, H 574 Health Sciences, Box 357650, Seattle, WA 98195, USA
Proc Natl Acad Sci U S A 97:13766-71. 2000....
- Experimental factors affecting PCR-based estimates of microbial species richness and evennessAnna Engelbrektson
Department of Energy Joint Genome Institute, Walnut Creek, CA, USA
ISME J 4:642-7. 2010..However, estimates of species evenness are consistent among different primer pairs targeting the same region. These results highlight the importance of experimental methodology when comparing diversity estimates across communities...
- Loop-mediated isothermal amplification (LAMP) of gene sequences and simple visual detection of productsNorihiro Tomita
Eiken Chemical Co, Ltd, Biochemical Research Laboratory, 1381 3 Shimoishigami, Ohtawara, Tochigi 324 0036, Japan
Nat Protoc 3:877-82. 2008..This detection method should be helpful in basic research on medicine and pharmacy, environmental hygiene, point-of-care testing and more...
- Accelerated reaction by loop-mediated isothermal amplification using loop primersK Nagamine
Eiken Chemical Co Ltd 1381 3 Shimoishigami, Ohtawara, Tochigi 324 0036, Japan
Mol Cell Probes 16:223-9. 2002..Since the total time of analysis including detection is less than 1h, this new method should facilitate genetic analysis, including genetic diagnosis in the clinical laboratory...
- DNA barcoding Australia's fish speciesRobert D Ward
CSIRO Marine and Atmospheric Research, GPO Box 1538, Hobart, Tasmania 7001, Australia
Philos Trans R Soc Lond B Biol Sci 360:1847-57. 2005..We conclude that cox1 sequencing, or 'barcoding', can be used to identify fish species...
- An efficient one-step site-directed and site-saturation mutagenesis protocolLei Zheng
Department of Chemistry and Biochemistry, University of Berne, Freiestrasse 3, CH 3012 Berne, Switzerland
Nucleic Acids Res 32:e115. 2004..This method should be useful to facilitate the preparation of high-quality site saturation libraries...
- Targeted deficiency or cytosolic truncation of the VE-cadherin gene in mice impairs VEGF-mediated endothelial survival and angiogenesisP Carmeliet
Center for Transgene Technology and Gene Therapy, Flanders Interuniversity Institute for Biotechnology, Leuven, Belgium
Cell 98:147-57. 1999..Thus, VE-cadherin/ beta-catenin signaling controls endothelial survival...
- Critical evaluation of two primers commonly used for amplification of bacterial 16S rRNA genesJeremy A Frank
Department of Microbiology, University of Illinois at Urbana Champaign, B103 C and LSL, 601 South Goodwin Ave, Urbana, IL 61801, USA
Appl Environ Microbiol 74:2461-70. 2008..particularly under stringent amplification conditions. Because our 27f formulation remains relatively simple, having seven distinct primer sequences, there is minimal loss of overall amplification efficiency and specificity...
- Sensitive, seminested PCR amplification of VP1 sequences for direct identification of all enterovirus serotypes from original clinical specimensW Allan Nix
Polio and Picornavirus Laboratory Branch, Division of Viral Diseases, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA
J Clin Microbiol 44:2698-704. 2006....
- Development and mapping of 2240 new SSR markers for rice (Oryza sativa L.)Susan R McCouch
Plant Breeding Dept, Cornell University, Ithaca, NY 14853 1901, USA
DNA Res 9:199-207. 2002..In combination with the pool of 500 previously mapped SSR markers, this release makes available a total of 2740 experimentally confirmed SSR markers for rice, or approximately one SSR every 157 kb...
- Assessment of soil microbial community structure by use of taxon-specific quantitative PCR assaysNoah Fierer
Department of Biology, Duke University, Durham, North Carolina 27708, USA
Appl Environ Microbiol 71:4117-20. 2005..Primers were thoroughly tested for specificity, and the method was applied to three distinct soils. The technique provides a rapid and robust index of microbial community structure...
- A third-generation lentivirus vector with a conditional packaging systemT Dull
Cell Genesys, Foster City, California 94404, USA
J Virol 72:8463-71. 1998..While the actual biosafety of the vector will ultimately be proven in vivo, the improved design presented here should facilitate testing of lentivirus vectors...
- Identification of mammalian microRNA host genes and transcription unitsAntony Rodriguez
Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, United Kingdom
Genome Res 14:1902-10. 2004....
- QuantPrime--a flexible tool for reliable high-throughput primer design for quantitative PCRSamuel Arvidsson
Max Planck Institute of Molecular Plant Physiology, Potsdam Golm, Germany
BMC Bioinformatics 9:465. 2008..Using currently available primer design tools, several interactive decisions have to be made, resulting in lengthy design processes with varying qualities of the assays...
- Efficient target-selected mutagenesis in zebrafishErno Wienholds
Hubrecht Laboratory, The Netherlands Institute for Developmental Biology, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands
Genome Res 13:2700-7. 2003..Furthermore,we show that TILLING can be used to detect the full spectrum of ENU-induced mutations in a vertebrate genome with the presence of many naturally occurring polymorphisms...
- CODEHOP (COnsensus-DEgenerate Hybrid Oligonucleotide Primer) PCR primer designTimothy M Rose
Department of Pathobiology, School of Public Health and Community Medicine, University of Washington, Seattle, WA 98195, USA
Nucleic Acids Res 31:3763-6. 2003..The CODEHOP designer (http://blocks.fhcrc.org/codehop.html) is linked to BlockMaker and the Multiple Alignment Processor within the Blocks Database World Wide Web (http://blocks.fhcrc.org)...
- RF cloning: a restriction-free method for inserting target genes into plasmidsFusinita van den Ent
MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK
J Biochem Biophys Methods 67:67-74. 2006..The absence of any alterations to the protein as well as the simplicity of both the primer design and the procedure itself makes it suitable for high-throughput expression and ideal for structural genomics...
- A universal DNA mini-barcode for biodiversity analysisIsabelle Meusnier
Biodiversity Institute of Ontario, University of Guelph, Guelph, Ontario, N1G 2W1, Canada
BMC Genomics 9:214. 2008..It may be difficult in practice, however, to retrieve a 650 bp fragment from archival specimens, (because of DNA degradation) or from environmental samples (where universal primers are needed)...
- Telomere measurement by quantitative PCRRichard M Cawthon
Department of Human Genetics, University of Utah, 15 N 2030 E, Room 2100, Salt Lake City, UT 84112, USA
Nucleic Acids Res 30:e47. 2002..This assay will facilitate investigations of the biology of telomeres and the roles they play in the molecular pathophysiology of diseases and aging...
- Disruption of the Cbfa2 gene causes necrosis and hemorrhaging in the central nervous system and blocks definitive hematopoiesisQ Wang
Department of Biochemistry, Dartmouth Medical School, Hanover, NH 03755, USA
Proc Natl Acad Sci U S A 93:3444-9. 1996..Definitive erythropoiesis and myelopoiesis do not occur in Cbfa2-deficient embryos, and disruption of one copy of the Cbfa2 gene significantly reduces the number of progenitors for erythroid and myeloid cells...
- Development of a real-time PCR assay for detection of Plasmodium falciparum, Plasmodium vivax, and Plasmodium ovale for routine clinical diagnosisF Perandin
Department of Laboratory Diagnosis, Section of Microbiology, University of Brescia, Spedali Civili, 25123 Brescia, Italy
J Clin Microbiol 42:1214-9. 2004..The assay can therefore be easily implemented in routine diagnostic malaria tests. Future studies are warranted to investigate the clinical value of this technique...
- Web-based primer design for single nucleotide polymorphism analysisMichael M Neff
Dept of Biology, Washington University, Campus Box 1137, St Louis, MO 63130 4899, USA
Trends Genet 18:613-5. 2002..Here we describe a web-based program, dCAPS Finder 2.0, that facilitates the design of mismatched PCR primers to create or remove a restriction endonuclease recognition site relative to the polymorphism being analyzed...
- A quantitative assay for HIV DNA integration in vivoS L Butler
Infectious Disease Laboratory, The Salk Institute for Biological Studies, La Jolla, California, USA
Nat Med 7:631-4. 2001..The integration assay employs a novel quantitative form of Alu-PCR that should be generally applicable to studies of integrating viruses and gene transfer vectors...
- Towards high-throughput molecular detection of Plasmodium: new approaches and molecular markersNicolas Steenkeste
Institut Pasteur du Cambodge, Laboratoire d Epidemiologie Moleculaire, Phnom Penh, Cambodia
Malar J 8:86. 2009..But sensitive diagnosis tools enabling to deal with a large number of samples are needed. The molecular detection approach offers a much higher sensitivity, and the flexibility to be automated and upgraded...
- Tumor spectrum analysis in p53-mutant miceT Jacks
Center for Cancer Research, Massachusetts Institute of Technology, Cambridge 02139
Curr Biol 4:1-7. 1994..The construction of mouse strains carrying germline mutations of p53 facilitates analysis of the function of p53 in normal cells and tumorigenesis...
- Bisulfite genomic sequencing: systematic investigation of critical experimental parametersC Grunau
Institute of Molecular Biotechnology, Department of Genome Analysis, Beutenbergstrasse 11, 07745 Jena, Germany
Nucleic Acids Res 29:E65-5. 2001..The recognition rates for cytosine (>/=97%) and 5-methylcytosine (>/=94%) were found to be identical for both templates...
- FIH-1: a novel protein that interacts with HIF-1alpha and VHL to mediate repression of HIF-1 transcriptional activityP C Mahon
Institute of Genetic Medicine, Departments of Pediatrics and Medicine, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21287 3914, USA
Genes Dev 15:2675-86. 2001..Involvement of VHL in association with FIH-1 provides a unifying mechanism for the modulation of HIF-1alpha protein stabilization and transcriptional activation in response to changes in cellular O(2) concentration...
- Applied genomics: data mining reveals species-specific malaria diagnostic targets more sensitive than 18S rRNAAllison Demas
Malaria Branch, Division of Parasitic Diseases and Malaria, Center for Global Health, Centers for Disease Control and Prevention, Atlanta, Georgia 30602, USA
J Clin Microbiol 49:2411-8. 2011..These novel targets provide a powerful alternative molecular diagnostic method for the detection of P. falciparum and P. vivax in conventional or multiplex PCR platforms...
- Double-joint PCR: a PCR-based molecular tool for gene manipulations in filamentous fungiJae Hyuk Yu
Department of Food Microbiology and Toxicology, The University of Wisconsin, Madison, WI 53706, USA
Fungal Genet Biol 41:973-81. 2004..To stream line the analysis of the transformants a relatively simple procedure for genomic DNA or total RNA isolation achieving approximately 100 samples/person/day is also presented...
- A soybean transcript map: gene distribution, haplotype and single-nucleotide polymorphism analysisIk Young Choi
Soybean Genomics and Improvement Laboratory, USDA, ARS, Beltsville, Maryland 20705, USA
Genetics 176:685-96. 2007....
- ITS as an environmental DNA barcode for fungi: an in silico approach reveals potential PCR biasesEva Bellemain
National Centre for Biosystematics, University of Oslo, Norway
BMC Microbiol 10:189. 2010..We performed in silico PCR analyses with commonly used primer combinations using various ITS datasets obtained from public databases as templates...
- The Vif protein of HIV triggers degradation of the human antiretroviral DNA deaminase APOBEC3GSilvestro G Conticello
Medical Research Council, Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, United Kingdom
Curr Biol 13:2009-13. 2003..Inhibitors of this interaction might therefore prove therapeutically useful in blocking Vif-mediated APOBEC3G destruction...
- Seed-specific over-expression of an Arabidopsis cDNA encoding a diacylglycerol acyltransferase enhances seed oil content and seed weightC Jako
Seed Oil Biotechnology Group, National Research Council of Canada, Plant Biotechnology Institute, 110 Gymnasium Place, Saskatoon, Saskatchewan S7N 0W9, Canada
Plant Physiol 126:861-74. 2001..The DGAT activity in developing seed of transgenic lines was enhanced by 10% to 70%. Thus, the current study confirms the important role of DGAT in regulating the quantity of seed triacylglycerols and the sink size in developing seeds...
- High-throughput stem-loop RT-qPCR miRNA expression profiling using minute amounts of input RNAPieter Mestdagh
Center for Medical Genetics, Ghent University Hospital, Ghent, Belgium
Nucleic Acids Res 36:e143. 2008..The high specificity of RT-qPCR together with a superior sensitivity makes this approach the method of choice for high-throughput miRNA expression profiling...
- Large deletions within the first intron in VRN-1 are associated with spring growth habit in barley and wheatDaolin Fu
Department of Plant Sciences, University of California, One Shields Av, Davis, CA 95616, USA
Mol Genet Genomics 273:54-65. 2005..Epistatic interactions between VRN-H2 and the VRN-H1 allele with the intron 1 deletion suggest that the deleted region may include a recognition site for the flowering repression mediated by the product of the VRN-H2 gene of barley...
- Maternal wnt11 activates the canonical wnt signaling pathway required for axis formation in Xenopus embryosQinghua Tao
Division of Developmental Biology, Cincinnati Children s Research Foundation, 3333 Burnet Avenue, Cincinnati, Ohio 45229, USA
Cell 120:857-71. 2005..Second, we show that activation requires the glycosyl transferase X.EXT1. Third, we find that the EGF-CFC protein, FRL1, is also essential and interacts with Wnt11 to activate canonical Wnt signaling...
- Specific and sensitive quantitative RT-PCR of miRNAs with DNA primersIngrid Balcells
Departament de Ciencia Animal i dels Aliments, Universitat Autonoma de Barcelona, 08193 Bellaterra, Spain
BMC Biotechnol 11:70. 2011..Due to the important biological role it is of great interest to quantitatively determine their expression level in different biological settings...
- Zebrafish Dkk1 functions in forebrain specification and axial mesendoderm formationH Hashimoto
Division of Molecular Oncology, Biomedical Research Center, Osaka University Graduate School of Medicine, 2 2 Yamada oka, Suita, Osaka, 565 0871, Japan
Dev Biol 217:138-52. 2000..These data indicate that Dkk1, expressed in dorsal mesendoderm, functions in the formation of both the anterior nervous system and the axial mesendoderm in zebrafish...
- BIPES, a cost-effective high-throughput method for assessing microbial diversityHong Wei Zhou
Department of Environmental Health, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou, Guangdong, China
ISME J 5:741-9. 2011..As a laborsaving and cost-effective method, BIPES can be routinely used to analyze the microbial ecology of both environmental and human microbiomes...
- Effect of primer mismatch, annealing temperature and PCR cycle number on 16S rRNA gene-targetting bacterial community analysisRita Sipos
Department of Microbiology, Eötvös Loránd University of Science, Budapest, Hungary
FEMS Microbiol Ecol 60:341-50. 2007..As a result of additional tests on environmental samples, the use of a low annealing temperature is recommended in order to significantly reduce preferential amplification while maintaining the specificity of PCR...
- Defects of B-cell lymphopoiesis and bone-marrow myelopoiesis in mice lacking the CXC chemokine PBSF/SDF-1T Nagasawa
Department of Immunology, Research Institute, Osaka Medical Center for Maternal and Child Health, Japan
Nature 382:635-8. 1996..In addition, the mutants had a cardiac ventricular septal defect. Hence, we have shown that the chemokine PBSF/SDF-1 has several essential functions in development...
- RTPrimerDB: the portal for real-time PCR primers and probesSteve Lefever
Center for Medical Genetics, Ghent University Hospital, De Pintelaan 185, 9000 Gent, Belgium
Nucleic Acids Res 37:D942-5. 2009..RTPrimerDB is a one-stop portal for high-quality and highly annotated real-time PCR assays...
- PCR as a confirmatory technique for laboratory diagnosis of malariaStephanie P Johnston
Division of Parasitic Diseases, M S F 36, Centers for Disease Control and Prevention, U S Department of Health and Human Services, 4770 Buford Highway NE, Atlanta, Georgia 30341 3724, USA
J Clin Microbiol 44:1087-9. 2006..in blood specimens. PCR was more sensitive than microscopy and capable of identifying malaria parasites at the species level when microscopy was equivocal...
- Plasticity in the organization and sequences of human KIR/ILT gene familiesM J Wilson
Immunology Division, Department of Pathology, Tennis Court Road, Cambridge CB2 1QP, United Kingdom
Proc Natl Acad Sci U S A 97:4778-83. 2000..KIR genesis was followed by extensive duplication/deletion as well as intergenic sequence exchange, reminiscent of MHC class I genes, which provide KIR ligands...
- Polymerase chain reaction primers miss half of rRNA microbial diversitySunhee Hong
Department of Biology, Northeastern University, Boston, MA, USA
ISME J 3:1365-73. 2009....
- Stem-cell protein Piwil2 is widely expressed in tumors and inhibits apoptosis through activation of Stat3/Bcl-XL pathwayJae Ho Lee
Institute of Human Genetics, University of Gottingen, 37073 Gottingen, Germany
Hum Mol Genet 15:201-11. 2006..Expression of Piwil2 in a wide variety of tumors could be a useful prognostic factor that could have also diagnostic and therapeutic implications...
- Bone marrow CD169+ macrophages promote the retention of hematopoietic stem and progenitor cells in the mesenchymal stem cell nicheAndrew Chow
Department of Gene and Cell Medicine, Mount Sinai School of Medicine, New York, NY 10029, USA
J Exp Med 208:261-71. 2011..Thus, strategies that target BM MΦ hold the potential to augment stem cell yields in patients that mobilize HSCs/progenitors poorly...
- Validation of housekeeping genes for gene expression studies in Symbiodinium exposed to thermal and light stressNedeljka N Rosic
Global Change Institute, University of Queensland, St Lucia, Brisbane, 4072, QLD, Australia
Mar Biotechnol (NY) 13:355-65. 2011..This study provides the first list of the HKGs and will provide a useful reference in future gene expression studies in symbiotic dinoflagellates...
- Uncultured archaea in deep marine subsurface sediments: have we caught them all?Andreas Teske
Department of Marine Sciences, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
ISME J 2:3-18. 2008....
- Detection of influenza A viruses from different species by PCR amplification of conserved sequences in the matrix geneR A Fouchier
National Influenza Center and Department of Virology, Erasmus University, Rotterdam, The Netherlands
J Clin Microbiol 38:4096-101. 2000..Comparative tests with throat swab samples from humans and fecal and cloacal swab samples from birds confirmed that the new PCR is faster and up to 100-fold more sensitive than classical virus isolation procedures...
- The head involution defective gene of Drosophila melanogaster functions in programmed cell deathM E Grether
Howard Hughes Medical Institute, Department of Brain and Cognitive Sciences, Cambridge, Massachusetts, USA
Genes Dev 9:1694-708. 1995..This phenotype can be suppressed completely by expression of the anti-apoptotic p35 protein from baculovirus, indicating that p35 may act genetically downstream from hid...
- Regulation of Gli2 and Gli3 activities by an amino-terminal repression domain: implication of Gli2 and Gli3 as primary mediators of Shh signalingH Sasaki
Laboratory of Developmental Biology, Institute for Molecular and Cellular Biology, Osaka University, Suita, Osaka 565 0871, Japan
Development 126:3915-24. 1999....
- Analysis of Australian fur seal diet by pyrosequencing prey DNA in faecesBruce E Deagle
Australian Marine Mammal Centre, Australian Antarctic Division, Tasmania, Australia
Mol Ecol 18:2022-38. 2009..The pyrosequencing approach presented significantly expands the capabilities of DNA-based methods of dietary analysis and is suitable for large-scale diet investigations on a broad range of animals...
- BARCRAWL and BARTAB: software tools for the design and implementation of barcoded primers for highly multiplexed DNA sequencingDaniel N Frank
Department of Molecular, University of Colorado, Boulder, CO 80309, USA
BMC Bioinformatics 10:362. 2009....
- Mapping of picoeucaryotes in marine ecosystems with quantitative PCR of the 18S rRNA geneFei Zhu
Station Biologique, UMR 7127 CNRS, INSU et Université Pierre et Marie Curie, Place George Teissier, 29680 Roscoff, France
FEMS Microbiol Ecol 52:79-92. 2005..Data interpretation must take into account primer specificity and the varying rRNA gene copy number among eukaryotes...
- Leptomycin B inactivates CRM1/exportin 1 by covalent modification at a cysteine residue in the central conserved regionN Kudo
Department of Biotechnology, Graduate School of Agriculture and Life Sciences, The University of Tokyo, Bunkyo ku, Tokyo 113 8657, Japan
Proc Natl Acad Sci U S A 96:9112-7. 1999..These results show that the single cysteine residue determines LMB sensitivity and is selectively alkylated by LMB, leading to CRM1 inactivation...
- Strategies for microsatellite isolation: a reviewL Zane
Dipartimento di Biologia, Università di Padova Via Ugo Bassi 58 B, 35121, Padova, Italy
Mol Ecol 11:1-16. 2002..In addition, we propose a fast and easy protocol which is a combination of different published methods...
- Abnormal blood vessel development and lethality in embryos lacking a single VEGF alleleP Carmeliet
Center for Transgene Technology and Gene Therapy, Flanders Interuniversity Institute for Biotechnology, Leuven, Belgium
Nature 380:435-9. 1996....
- Hydrazine synthase, a unique phylomarker with which to study the presence and biodiversity of anammox bacteriaHarry R Harhangi
Department of Microbiology, Institute for Water and Wetland Research IWWR, Radboud University Nijmegen, Nijmegen, The Netherlands
Appl Environ Microbiol 78:752-8. 2012....
- A method for studying protistan diversity using massively parallel sequencing of V9 hypervariable regions of small-subunit ribosomal RNA genesLinda A Amaral-Zettler
The Josephine Bay Paul Center for Comparative Molecular Biology and Evolution, Marine Biological Laboratory, Woods Hole, MA, USA
PLoS ONE 4:e6372. 2009..Recent advances in pyrosequencing technology provide read lengths of up to 240 nucleotides. Amplicon pyrosequencing can now be applied to longer variable regions of the SSU rRNA gene including the V9 region in eukaryotes...
- Quantitative analysis of diverse Lactobacillus species present in advanced dental cariesRoy Byun
Institute of Dental Research, Westmead Centre for Oral Health, P O Box 533, Wentworthville, NSW 2145, Australia
J Clin Microbiol 42:3128-36. 2004..gasseri and L. ultunensis than of the other prevalent species. The findings provide a basis for further characterization of the pathogenicity of Lactobacillus spp. in the context of extension of the carious lesion...
- A method for the absolute quantification of cDNA using real-time PCRJoseph A Whelan
Onyvax Ltd, St George s Hospital Medical School, Cranmer Terrace, London SW17 0RE, UK
J Immunol Methods 278:261-9. 2003..Although our interest has been cytokine quantification, we believe that this technique is widely applicable to the majority of real-time PCR applications...
- BatchPrimer3: a high throughput web application for PCR and sequencing primer designFrank M You
Department of Plant Sciences, University of California, CA 95616, USA
BMC Bioinformatics 9:253. 2008..In particular, most programs lack batch primer design capability. Such a high-throughput software tool for designing SSR flanking primers and SNP genotyping primers is increasingly demanded...
- Tumor-associated endothelial cells with cytogenetic abnormalitiesKyoko Hida
Vascular Biology Program, Department of Surgery, Children s Hospital and Harvard Medical School, Boston, Massachusetts 02115, USA
Cancer Res 64:8249-55. 2004..FISH analysis of tumor sections also showed endothelial cell aneuploidy. We conclude that tumor endothelial cells can acquire cytogenetic abnormalities while in the tumor microenvironment...
- Osteoclast differentiation independent of the TRANCE-RANK-TRAF6 axisNacksung Kim
Medical Research Center for Gene Regulation, Chonnam National University Medical School, Gwangju, Korea
J Exp Med 202:589-95. 2005..We provide direct evidence against the current paradigm that the TRANCE-RANK-TRAF6 pathway is essential for osteoclast differentiation and suggest the potential existence of alternative routes for osteoclast differentiation...
- Microsatellites as DNA markers in cultivated peanut (Arachis hypogaea L.)Guohao He
Tuskegee University, Tuskegee, AL 36088, USA
BMC Plant Biol 3:3. 2003..Genomic research of cultivated peanut has lagged behind other crop species because of the paucity of polymorphic DNA markers found in this crop. It is necessary to identify additional DNA markers for further genetic research in peanut...
- In silico analysis on frequency and distribution of microsatellites in ESTs of some cereal speciesRajeev K Varshney
Institute of Plant Genetics and Crop Plant Research IPK, Corrensstrasse 3, D 06466 Gatersleben, Germany
Cell Mol Biol Lett 7:537-46. 2002....
- Capsular polysaccharide synthesis regions in Klebsiella pneumoniae serotype K57 and a new capsular serotypeYi Jiun Pan
Department of Microbiology, National Taiwan University College of Medicine, Taipei, Taiwan
J Clin Microbiol 46:2231-40. 2008..We conclude that sequences from these two cps regions are very useful in detecting K57 and the new cps genotype...
- Use of sequence data from rainbow trout and Atlantic salmon for SNP detection in Pacific salmonChristian T Smith
Gene Conservation Laboratory, Division of Commercial Fisheries, Alaska Department of Fish and Game, 333 Raspberry Road, Anchorage, AK 99518, USA
Mol Ecol 14:4193-203. 2005..While the costs of DNA sequencing are still significant, these must be compared to the costs of using other marker classes for a given application...
- LINE-1 hypomethylation in cancer is highly variable and inversely correlated with microsatellite instabilityMarcos R H Estecio
Department of Leukemia, University of Texas M D Anderson Cancer Center, Houston, Texas, United States of America
PLoS ONE 2:e399. 2007..This study was performed to determine the relationship between DNA hypomethylation, hypermethylation and microsatellite instability in cancer...
- The use of coded PCR primers enables high-throughput sequencing of multiple homolog amplification products by 454 parallel sequencingJonas Binladen
Center for Ancient Genetics, Institute of Biology, University of Copenhagen, Copenhagen, Denmark
PLoS ONE 2:e197. 2007....
- Characterization of Dicer-deficient murine embryonic stem cellsElizabeth P Murchison
Watson School of Biological Sciences, Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, NY 11724, USA
Proc Natl Acad Sci U S A 102:12135-40. 2005..These studies provide a conditional model of RNAi deficiency in mammals that will permit the dissection of the biological roles of the RNAi machinery in cultured mammalian cells...
- Robustness of a loop-mediated isothermal amplification reaction for diagnostic applicationsPatrice Francois
Genomic Research Laboratory, University Hospitals of Geneva, Service of Infectious Diseases, Geneva, Switzerland
FEMS Immunol Med Microbiol 62:41-8. 2011..Our results demonstrate that LAMP is not only easy to use, but is also a very robust, innovative and powerful molecular diagnostic method for both industrialized and developing countries...
- Cost-effective, species-specific microsatellite development for the endangered Dwarf Bulrush (Typha minima) using next-generation sequencing technologyDaniela Csencsics
Research Unit Ecological Genetics and Evolution, Swiss Federal Research Institute WSL, Zürcherstrasse 111, CH 8903 Birmensdorf, Switzerland
J Hered 101:789-93. 2010....
- Amplifying mRNA Sequences from Small Amounts of TissuePaul Siebert; Fiscal Year: 2006..In the Phase I Research we propose to develop such a method, and utilize a set on non-random DNA primers for the synthesis and amplification of cDNA...
- Providing the $1000 genome via improved single molecule sequencingJOHN FENTON THOMPSON; Fiscal Year: 2010..By developing an ordered surface of DNA primers, up to 5x increase in sequencing yield is possible...
- DNA sequencing by ELFSE on microfluidic devicesAnnelise Barron; Fiscal Year: 2006..Optimized protocols for conjugation of drag-tags to DNA primers, cycle sequencing, and sample cleanup will be developed...
- ADDRESSING THE ISSUE OF GAP REGIONS IN GENOME SEQUENCINGJohn SantaLucia; Fiscal Year: 2001..The Watson-Crick pairing rules are not sufficient to design robust DNA primers or hybridization arrays...
- Novel Real Time Isothermal DNA Amplification/DetectionWILLIAM MACCONNELL; Fiscal Year: 2005..the "hairpin loop concatamer DNA amplification" (HLCA) process which incorporates self-priming hairpin oligo DNA primers into linear DNA during the initial rounds of replication (5'8)...
- POST-TRANSCRIPTIONAL MODIFICATIONS IN RIBOSOMAL RNADavid Jemiolo; Fiscal Year: 1990..Modifications will be located by sequencing rRNA using DNA primers and reverse transcriptase...
- DNA BASE CALLING USING HETERODYNE FLUORESCENCE DETECTIONKristen Peterson; Fiscal Year: 1999..The Phase I project will demonstrate our approach by identifying and distinguishing DNA primers tagged with recently developed dyes that absorb red light and have lifetimes of l to 5 nsecs...
- Novel Real Time Isothermal DNA Amplification/DetectionWILLIAM MACCONNELL; Fiscal Year: 2003..the "hairpin loop concatamer DNA amplification" (HLCA) process, which incorporates self-priming hairpin oligo DNA primers into linear DNA during the initial rounds of replication...
- INT-1 PROTO-ONCOGENE FAMILYGREGORY SHACKLEFORD; Fiscal Year: 1993..describes: 1) The polymerase chain reaction will be used to amplify DNA from genes in the int-1 family using DNA primers corresponding to conserved regions in the 3 presently known members...
- Multiplexed Reactive Sequencing of DNAPeter Williams; Fiscal Year: 2007DESCRIPTION (provided by applicant): A new approach to DNA sequencing is proposed. DNA primers synthesized to be complementary to specific sequences on targeted genes will be covalently tethered in known locations in an array on the ..
- In vitro culture and transformation of Wolbachia from mosquitoesAnn Fallon; Fiscal Year: 2007..unreadable] [unreadable] [unreadable]..
- Prognostic Significance of DNA and Histone MethylationChandrika Piyathilake; Fiscal Year: 2007..Investigator talent, equipment, facilities and access to study subjects at UAB are ideal for conducting this study. ..
- Ethanol Effects on Human Astroglial Chemokine ExpressionRandall Davis; Fiscal Year: 2007..unreadable] [unreadable] [unreadable]..
- GBS: Epidemiologic Characterization for Gene DiscoveryBetsy Foxman; Fiscal Year: 2007..A better understanding of the molecular epidemiology and the identification of new GBS virulence genes will facilitate the discover of new therapies and prevention strategies for GBS disease. ..
- HU-UPCI Cancer Education/Career Development PartnershipRichard Steinman; Fiscal Year: 2006..abstract_text> ..
- Mechanisms and Functions of Human SulfotransferasesGuangping Chen; Fiscal Year: 2009..The knowledge will be important in understanding drug side effect, drug-drug interaction, drug development, and the potential roles SULTs play in cancer prevention and causation. ..
- Oral and Vaginal Microbes, Human Genotype, and Preterm BirthDawn P Misra; Fiscal Year: 2010..For this proposal we have the great advantage of exploring these hypotheses using already collected specimens and data as part of completed studies of preterm birth. ..
- Muscle protein synthesis and gene expression in myostatin-deficient miceStephen L Welle; Fiscal Year: 2010..Knowledge of the physiological and molecular effects of reducing myostatin activity in mature muscle will reveal potential benefits and limitations of anti-myostatin therapy. ..
- HPV Clearance by Folic Acid SupplementationChandrika J Piyathilake; Fiscal Year: 2010..Because this study will be conducted among women positive for HPV 16, the most prevalent and powerful causative agent of CC, the public health significance of this study could be enormous and will complement HPV vaccine studies. ..
- Safety of Nicotine Reduction StrategyNeal Benowitz; Fiscal Year: 2009..The proposed research is needed to guide governmental regulators as to how best to regulate nicotine in cigarettes and is an essential first step in examining the feasibility of a national regulation program. ..
- Redox-sensitive developmental pathways and gene regulatory networksJAMES COFFMAN; Fiscal Year: 2009..Finally, the Comparative Toxicogenomics Database (CTD) at MDIBL will be used to determine the relevance of the pathways discovered in sea urchins to human health, and to generate hypotheses that might explain specific human diseases. ..
- Control of Cell Proliferation by Runx ProteinsJAMES COFFMAN; Fiscal Year: 2009..purpuratus genome sequence, which will greatly facilitate both the cis-regulatory analysis of genes and the identification of purified proteins by mass spectrometry. ..
- Database of Functional SNPs in Cancer-Related Environmentally Responsive GenesYong Zhu; Fiscal Year: 2009..Findings from this study will enhance the use of SNPs in future phenotypic studies and molecular epidemiologic approaches to address questions related to gene-environment interaction in disease risk estimate. ..
- The Role of Mitochondrial DNA Alterations in CancerLee Jun Wong; Fiscal Year: 2008..Results from this research project will help us understand the functional role of mitochondrial DNA alterations in cancer and identify potential novel targets for more effective therapeutic development. ..
- HUMAN OXIDATION RESISTANCE GENESMichael Volkert; Fiscal Year: 2007..abstract_text> ..
- Role of Retinal Microvasculature in Posterior UveitisJustine Smith; Fiscal Year: 2006..The studies should also have implications for unrelated diseases affecting the retinal circulation, as well as tissue-specific inflammations and infections at other body sites. [unreadable] [unreadable]..
- NEUROTROPIC CYTOMEGALOVIRUS DURING IMMUNOSUPPRESSIONJON REUTER; Fiscal Year: 2006..abstract_text> ..
- NUCLEOTIDE DONORS AFFECTING GLYCOSYLATION PATHWAYSAjit Varki; Fiscal Year: 2003....
- ACTIVE SITE STUDIES OF HUMAN SULFOTRANSFERASESGuangping Chen; Fiscal Year: 2003..The characterization of the active sites of STs will also help to find mechanism-based specific inhibitors/activators for use in studying the physiological functions of STs and designing new drugs. ..
- CHANGES IN GENE EXPRESSION IN SENESCENT MUSCLEStephen Welle; Fiscal Year: 2003..abstract_text> ..
- Ethanol and NOS2 Gene Expression in Human AstrocytesRandall Davis; Fiscal Year: 2004..Use of deletion constructs will help identify ethanol-sensitive regions for future studies on trans-acting factors involved in the action of ethanol. ..
- SYNTHESIS AND DEGRADATION OF RIBOSOMES IN THE MOSQUITOAnn Fallon; Fiscal Year: 2004..These studies provide cloned genes, molecular approaches, and characterization of regulatory processes that can potentially be manipulated to interfere with disease transmission by blood-feeding arthropods. ..
- MOSQUITO IMMUNITY FUNCTIONSAnn Fallon; Fiscal Year: 2003..The work will include identification of exon-intron organization and recovery of upstream flanking DNA, which will be analyzed for potential regulatory elements. ..
- FUNCTION AND REGULATION OF THE E COLI AIDB GENESMichael Volkert; Fiscal Year: 2002..During the proposed funding period, I propose to characterize this factor further by genetic and biochemical means and to compare its induction mechanism with that of meAda. ..
- Prognostic Significance of Altered DNA MethylationChandrika Piyathilake; Fiscal Year: 2002..A newly developed and tested immunohistochemical technique, which measures the degree of global methylation in intact and specific types of cells, will be used to evaluate global DNA methylation in the proposed study. ..
- Effects of Folate Fortification on Cancer Prevention an*Chandrika Piyathilake; Fiscal Year: 2005....
- ECDYSONE EFFECTS ON MOSQUITO CELL PROLIFERATIONAnn Fallon; Fiscal Year: 2001..Results of this study will provide novel insights into the effects of 20E on cell growth and proliferation, which are directly relevant to efforts to control vector-borne disease by genetic manipulation of insect vectors. ..
- CHEMOKINES/CYTOKINES AND RECEPTORS IN STEM CELL HOMINGHal Broxmeyer; Fiscal Year: 2005..These studies should clarity the relevance of SDF-1 and CXCR4 for homing/engraftment and mobilization of primary stem/progenitor cells. ..
- MOLECULAR CONTRIBUTORS TO STEM CELL QUIESCENCERichard Steinman; Fiscal Year: 2003..Insights gained from these studies should have a significant impact on gene therapy strategies and should generate novel tools for the study of leukemias and myelodysplastic syndromes. ..
- A Nucleosomal Biosensor for Identification and Isolation of Nuclear Hormone RecepRichard Steinman; Fiscal Year: 2008..This biosensor should be more rapid and versatile than cell-based reporter gene assays and more informative than assays that measure only ligand/receptor binding. [unreadable] [unreadable] [unreadable]..
- MGB Eclipse Probe Detection of Category A OrganismsIrina Afonina; Fiscal Year: 2003..In Phase II, we will expand the number of Category A organisms and improve throughput by multiplexing. In addition, commercial prototype MGB Eclipse(TM) probe assays will be developed for each of these organisms. ..
- Attenuation of astroglial chemokine expression by beta-funaltrexamine: implicatioRandall Davis; Fiscal Year: 2008..This training will lend to the success of these students in their future careers in health related professions. [unreadable] [unreadable] [unreadable]..
- Chemokines in host defense to Campylobacter jejuniMICHAEL DWINELL; Fiscal Year: 2004..jejuni infection are central to the development of preventative therapeutic strategies to modulate host-pathogen interactions to favor the host. ..
- Hypoxia and the Role of HIF-1 in the Ischemic TestisMichael Palladino; Fiscal Year: 2005..abstract_text> ..
- Mobilization of Candida albicans BiofilmsPeter Suci; Fiscal Year: 2007..albicans biofilm communities, it will provide the basis for development of methods for screening for adjuvant agents that will inhibit dissemination of these fungal infections. [unreadable] [unreadable] [unreadable]..
- Inhibition of NF-KB to Facilitate Islet TransplantationPaul Robbins; Fiscal Year: 2003..The successful completion of the proposed studies should determine if NF-kB inhibition in islets by peptide and gene mediated transduction is able to improve islet viability prior to and post-transplantation. ..
- REGULATION OF TRANSCRIPTION BY THE RB ANTIONCOGENEPaul Robbins; Fiscal Year: 2002..The experiments described in the proposal are expected to provide important insights into the mechanism of how RB and cyclin D1 function to regulate transcription directly, cell differentiation, and tumorigenesis. ..
- Improved Brucella Vaccine StrainsThomas Ficht; Fiscal Year: 2009..abstract_text> ..
- 2008 Viral Vectors for Gene Therapy, The Science of Gordon Research ConferencePaul Robbins; Fiscal Year: 2008..End of Abstract) [unreadable] [unreadable] [unreadable]..
- Mechanisms of Regulation of GLUT4 ExpressionANN OLSON; Fiscal Year: 2005..abstract_text> ..
- Neurogenesis as a compensatory mechanism to regulate energy balanceALLISON XU; Fiscal Year: 2008..The identification of neuronal substrates critical for body weight regulation will lay the groundwork for future therapeutic interventions designed to target these specific neurons. [unreadable] [unreadable] [unreadable]..
- Insulin Regulation of Adipocyte MicrotubulesANN OLSON; Fiscal Year: 2008..unreadable] [unreadable]..
- Dendritic Cells For Prevention of Islet Graft RejectionKarl Womer; Fiscal Year: 2008..Finally, our studies will be important to the development of clinical strategies designed to prolong the survival of islet cell transplants. ..