antisense rna

Summary

Summary: RNA molecules which hybridize to complementary sequences in either RNA or DNA altering the function of the latter. Endogenous antisense RNAs function as regulators of gene expression by a variety of mechanisms. Synthetic antisense RNAs are used to effect the functioning of specific genes for investigative or therapeutic purposes.

Top Publications

  1. ncbi Antisense transcription in the mammalian transcriptome
    S Katayama
    Laboratory for Genome Exploration Research Group, RIKEN Genomic Sciences Centre GSC, RIKEN Yokohama Institute, 1 7 22 Suehiro cho, Tsurumi ku, Yokohama, 230 0045, Japan
    Science 309:1564-6. 2005
  2. ncbi Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans
    A Fire
    Carnegie Institution of Washington, Department of Embryology, Baltimore, Maryland 21210, USA
    Nature 391:806-11. 1998
  3. pmc Epigenetic silencing of tumour suppressor gene p15 by its antisense RNA
    Wenqiang Yu
    Center for Epigenetics and Department of Medicine, Johns Hopkins University School of Medicine, 720 Rutland Avenue, Baltimore, Maryland 21205, USA
    Nature 451:202-6. 2008
  4. pmc Coupled degradation of a small regulatory RNA and its mRNA targets in Escherichia coli
    Eric Massé
    Laboratory of Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Genes Dev 17:2374-83. 2003
  5. pmc Nascent RNA sequencing reveals widespread pausing and divergent initiation at human promoters
    Leighton J Core
    Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853, USA
    Science 322:1845-8. 2008
  6. ncbi Double-stranded RNA-mediated silencing of genomic tandem repeats and transposable elements in the D. melanogaster germline
    A A Aravin
    Department of Animal Molecular Genetics, Institute of Molecular Genetics, 123182, Moscow, Russia
    Curr Biol 11:1017-27. 2001
  7. ncbi An antisense RNA inhibits translation by competing with standby ribosomes
    Fabien Darfeuille
    Department of Cell and Molecular Biology, Biomedical Center, Uppsala University, Box 596, S 75124 Uppsala, Sweden
    Mol Cell 26:381-92. 2007
  8. ncbi Human Argonaute2 mediates RNA cleavage targeted by miRNAs and siRNAs
    Gunter Meister
    Laboratory of RNA Molecular Biology, The Rockefeller University, 1230 York Avenue, Box 186, New York, NY 10021, USA
    Mol Cell 15:185-97. 2004
  9. pmc Small untranslated RNA antitoxin in Bacillus subtilis
    Jessica M Silvaggi
    Department of Molecular and Cellular Biology, The Biological Laboratories, 16 Divinity Ave, Harvard University, Cambridge, MA 02138, USA
    J Bacteriol 187:6641-50. 2005
  10. pmc Hfq-dependent regulation of OmpA synthesis is mediated by an antisense RNA
    Klas I Udekwu
    Department of Cell and Molecular Biology, Uppsala University, S 75124 Uppsala, Sweden
    Genes Dev 19:2355-66. 2005

Research Grants

  1. Small Regulatory RNAs of Coxiella burnetii - The Agent of Q Fever
    JAMES MICHAEL BATTISTI; Fiscal Year: 2013
  2. Heterochromatin on the human inactive X chromosome
    Brian P Chadwick; Fiscal Year: 2012
  3. Genetically Specific Therapy Against Pathogenic Bacteria
    Stanley R Maloy; Fiscal Year: 2013
  4. Epigenomics of SLE
    Kathleen E Sullivan; Fiscal Year: 2013
  5. BP1 and Nuclear Hormone Signaling in Breast Cancer
    JOSEPH PINZONE; Fiscal Year: 2009
  6. Non-coding RNAs for Epigenetic Transcriptional Silencing in Prostate Cancer
    Ming Ming Zhou; Fiscal Year: 2013
  7. Identification and study of the vascular disease gene at 9p21.3
    Thomas Quertermous; Fiscal Year: 2013
  8. Global analysis of mRNA polarization in migrating endothelial cells
    Paul L Fox; Fiscal Year: 2010
  9. Regulation of the Na/K Pump by RNA Editing
    Joshua J C Rosenthal; Fiscal Year: 2013
  10. Mechanism of UBE3A Imprint in Neurodevelopment
    WESTON THOMAS POWELL; Fiscal Year: 2013

Detail Information

Publications318 found, 100 shown here

  1. ncbi Antisense transcription in the mammalian transcriptome
    S Katayama
    Laboratory for Genome Exploration Research Group, RIKEN Genomic Sciences Centre GSC, RIKEN Yokohama Institute, 1 7 22 Suehiro cho, Tsurumi ku, Yokohama, 230 0045, Japan
    Science 309:1564-6. 2005
    ..We present experimental evidence that perturbation of an antisense RNA can alter the expression of sense messenger RNAs, suggesting that antisense transcription contributes to ..
  2. ncbi Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans
    A Fire
    Carnegie Institution of Washington, Department of Embryology, Baltimore, Maryland 21210, USA
    Nature 391:806-11. 1998
    ....
  3. pmc Epigenetic silencing of tumour suppressor gene p15 by its antisense RNA
    Wenqiang Yu
    Center for Epigenetics and Department of Medicine, Johns Hopkins University School of Medicine, 720 Rutland Avenue, Baltimore, Maryland 21205, USA
    Nature 451:202-6. 2008
    ..transcriptome analysis shows that up to 70% of transcripts have antisense partners and that perturbation of antisense RNA can alter the expression of the sense gene...
  4. pmc Coupled degradation of a small regulatory RNA and its mRNA targets in Escherichia coli
    Eric Massé
    Laboratory of Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Genes Dev 17:2374-83. 2003
    ..Thus, this large class of regulatory RNAs share an unexpected intrinsic mechanism for shutting off their action...
  5. pmc Nascent RNA sequencing reveals widespread pausing and divergent initiation at human promoters
    Leighton J Core
    Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853, USA
    Science 322:1845-8. 2008
    ..These results imply that the interplay between polymerases and regulators over broad promoter regions dictates the orientation and efficiency of productive transcription...
  6. ncbi Double-stranded RNA-mediated silencing of genomic tandem repeats and transposable elements in the D. melanogaster germline
    A A Aravin
    Department of Animal Molecular Genetics, Institute of Molecular Genetics, 123182, Moscow, Russia
    Curr Biol 11:1017-27. 2001
    ..The analysis of Caenorhabditis elegans mutants impaired with dsRNA-mediated silencing and studies in plants implicate a biological role of dsRNA-mediated silencing as a transposon-repression and antiviral mechanism...
  7. ncbi An antisense RNA inhibits translation by competing with standby ribosomes
    Fabien Darfeuille
    Department of Cell and Molecular Biology, Biomedical Center, Uppsala University, Box 596, S 75124 Uppsala, Sweden
    Mol Cell 26:381-92. 2007
    ..We propose a mechanism by which an antisense RNA inhibits translation without binding directly to a TIR...
  8. ncbi Human Argonaute2 mediates RNA cleavage targeted by miRNAs and siRNAs
    Gunter Meister
    Laboratory of RNA Molecular Biology, The Rockefeller University, 1230 York Avenue, Box 186, New York, NY 10021, USA
    Mol Cell 15:185-97. 2004
    ..The specific role of Ago2 in guiding target RNA cleavage was confirmed independently by siRNA-based depletion of individual Ago members in combination with a sensitive positive-readout reporter assay...
  9. pmc Small untranslated RNA antitoxin in Bacillus subtilis
    Jessica M Silvaggi
    Department of Molecular and Cellular Biology, The Biological Laboratories, 16 Divinity Ave, Harvard University, Cambridge, MA 02138, USA
    J Bacteriol 187:6641-50. 2005
    ..report the identification of a TA module in the chromosome of Bacillus subtilis in which the antitoxin is an antisense RNA. The antitoxin, which is called RatA (for RNA antitoxin A), is a small (222 nucleotides), untranslated RNA ..
  10. pmc Hfq-dependent regulation of OmpA synthesis is mediated by an antisense RNA
    Klas I Udekwu
    Department of Cell and Molecular Biology, Uppsala University, S 75124 Uppsala, Sweden
    Genes Dev 19:2355-66. 2005
    ..MicA requires the presence of the Hfq protein, although the mechanistic basis for this remains unclear...
  11. pmc RNase E-based ribonucleoprotein complexes: mechanical basis of mRNA destabilization mediated by bacterial noncoding RNAs
    Teppei Morita
    Division of Biological Science, Graduate School of Science, Nagoya University, Chikusa, Nagoya 464 8602, Japan
    Genes Dev 19:2176-86. 2005
    ..The formation of ribonucleoprotein complexes containing RNases could be a general way by which small RNAs destabilize target mRNAs in both prokaryotes and eukaryotes...
  12. ncbi Analysis of the mouse transcriptome based on functional annotation of 60,770 full-length cDNAs
    Y Okazaki
    1 Laboratory for Genome Exploration Research Group, RIKEN Genomic Sciences Center, RIKEN Yokohama Institute 1 7 22 Suehiro cho, Tsurumi ku, Yokohama, Kanagawa, 230 0045, Japan
    Nature 420:563-73. 2002
    ..The present work, completely supported by physical clones, provides the most comprehensive survey of a mammalian transcriptome so far, and is a valuable resource for functional genomics...
  13. pmc An internal antisense RNA regulates expression of the photosynthesis gene isiA
    Ulf Dühring
    Department of Plant Biochemistry, Institute of Biology, Humboldt University Berlin, Chausseestrasse 117, D 10115 Berlin, Germany
    Proc Natl Acad Sci U S A 103:7054-8. 2006
    ..Here, we show that this process is controlled by IsrR (iron stress-repressed RNA), a cis-encoded antisense RNA transcribed from the isiA noncoding strand...
  14. pmc Evidence for a major role of antisense RNAs in cyanobacterial gene regulation
    Jens Georg
    Faculty of Biology and Freiburg Initiative in Systems Biology, University of Freiburg, Freiburg 79104, Germany
    Mol Syst Biol 5:305. 2009
    ..Extrapolated to the whole genome, approximately 10% of all genes in Synechocystis are influenced by asRNAs. Thus, chromosomally encoded asRNAs may have an important function in eubacterial regulatory networks...
  15. pmc An antisense RNA that governs the expression kinetics of a multifunctional virulence gene
    Eun Jin Lee
    Department of Molecular Microbiology, Howard Hughes Medical Institute, Washington University School of Medicine, Campus Box 8230, 660 S Euclid Ave, St Louis, MO 63110, USA
    Mol Microbiol 76:1020-33. 2010
    ..We now report the identification of a cis-encoded 1.2 kb long antisense RNA - termed AmgR - that is complementary to the mgtC portion of the mgtCBR polycistronic message from Salmonella ..
  16. pmc A novel H19 antisense RNA overexpressed in breast cancer contributes to paternal IGF2 expression
    Nathalie Berteaux
    INSERM U908, Universite des Sciences et Technologies de Lille, Batiment SN3, 59655 Villeneuve d Ascq cedex, France
    Mol Cell Biol 28:6731-45. 2008
    ..Moreover, the transcript is stabilized in breast cancer cells and overexpressed in human breast tumors. Finally, knockdown experiments showed that, in humans, 91H, rather than affecting H19 expression, regulates IGF2 expression in trans...
  17. pmc Divergent transcription from active promoters
    Amy C Seila
    Koch Institute, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Science 322:1849-51. 2008
    ..These results suggest that divergent transcription over short distances is common for active promoters and may help promoter regions maintain a state poised for subsequent regulation...
  18. pmc Amplification of AKT2 in human pancreatic cells and inhibition of AKT2 expression and tumorigenicity by antisense RNA
    J Q Cheng
    Department of Medical Oncology, Fox Chase Cancer Center, Philadelphia, PA 19111, USA
    Proc Natl Acad Sci U S A 93:3636-41. 1996
    ..The expression of AKT2 protein in these clones was greatly decreased by the antisense RNA. Furthermore, tumorigenicity in nude mice was markedly reduced in PANC1 cells expressing antisense AKT2 RNA...
  19. pmc Endogenous siRNAs derived from a pair of natural cis-antisense transcripts regulate salt tolerance in Arabidopsis
    Omar Borsani
    Institute for Integrative Genome Biology and Department of Botany and Plant Sciences, University of California, Riverside, CA 92521, USA
    Cell 123:1279-91. 2005
    ....
  20. ncbi The imprinted antisense RNA at the Igf2r locus overlaps but does not imprint Mas1
    R Lyle
    The Netherlands Cancer Institute H5, Amsterdam, The Netherlands
    Nat Genet 25:19-21. 2000
    ..imprint was shown in a transgenic model to be essential for Igf2r imprinting and for the production of an antisense RNA from the paternal allele...
  21. ncbi RNA exosome depletion reveals transcription upstream of active human promoters
    Pascal Preker
    Centre for mRNP Biogenesis and Metabolism, Department of Molecular Biology, C F Møllers Alle, Building 1130, Aarhus University, Denmark
    Science 322:1851-4. 2008
    ..We propose that PROMPT transcription is a common characteristic of RNA polymerase II (RNAPII) transcribed genes with a possible regulatory potential...
  22. ncbi Antisense transcription controls cell fate in Saccharomyces cerevisiae
    Cintia F Hongay
    Whitehead Institute for Biomedical Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Cell 127:735-45. 2006
    ..Our data are consistent with transcription interference as a regulatory mechanism at the IME4 locus that determines cell fate...
  23. pmc S-box and T-box riboswitches and antisense RNA control a sulfur metabolic operon of Clostridium acetobutylicum
    Gaelle André
    Genetics of Bacterial Genomes, Pasteur Institute, CNRS URA2171, 25 rue du Dr Roux, 75724 Paris Cedex 15, France
    Nucleic Acids Res 36:5955-69. 2008
    ..We proposed a regulatory model in which the antisense RNA controlled the expression of the ubiG operon in cis via transcriptional interference at the ubiG locus.
  24. pmc Staphylococcus aureus RNAIII coordinately represses the synthesis of virulence factors and the transcription regulator Rot by an antisense mechanism
    Sandrine Boisset
    Institut National pour la Recherche Médicale INSERM E0230, Universite Lyon 1, Centre National de Référence des Staphylocoques, Faculte Laennec, Lyon, F 69008, France
    Genes Dev 21:1353-66. 2007
    ..We demonstrate that the 3' domain acts primarily as an antisense RNA and rapidly anneals to these mRNAs, forming long RNA duplexes...
  25. ncbi Antisense RNA stabilization induces transcriptional gene silencing via histone deacetylation in S. cerevisiae
    Jurgi Camblong
    Department of Cell Biology, Sciences III, University of Geneva, 30 quai E Ansermet, 1211 Geneva 4, Switzerland
    Cell 131:706-17. 2007
    ..However, histone deacetylation is restricted to PHO84, suggesting that Hda1 activity depends on antisense RNA. Accordingly, the knockdown of antisense production prevents PHO84 gene repression, even in the absence of ..
  26. ncbi Developmentally regulated piRNA clusters implicate MILI in transposon control
    Alexei A Aravin
    Watson School of Biological Sciences, Cold Spring Harbor Laboratory, Howard Hughes Medical Institute HHMI, 1 Bungtown Road, Cold Spring Harbor, NY 11724, USA
    Science 316:744-7. 2007
    ..Mili mutants derepress LINE-1 (L1) and intracisternal A particle and lose DNA methylation of L1 elements, demonstrating an evolutionarily conserved role for PIWI proteins in transposon suppression...
  27. ncbi Single-stranded antisense siRNAs guide target RNA cleavage in RNAi
    Javier Martinez
    Department of Cellular Biochemistry, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, D 37077, Gottingen, Germany
    Cell 110:563-74. 2002
    ..Single-stranded antisense siRNAs are also effectively silencing genes in HeLa cells, especially when 5'-phosphorylated, and expand the repertoire of RNA reagents suitable for gene targeting...
  28. ncbi RNAi: double-stranded RNA directs the ATP-dependent cleavage of mRNA at 21 to 23 nucleotide intervals
    P D Zamore
    Department of Biochemistry and Molecular Biology, University of Massachusetts Medical School, Worcester 01655, USA
    Cell 101:25-33. 2000
    ..Cleavage occurs at sites 21-23 nucleotides apart, the same interval observed for the dsRNA itself, suggesting that the 21-23 nucleotide fragments from the dsRNA are guiding mRNA cleavage...
  29. pmc Evidence for antisense transcription associated with microRNA target mRNAs in Arabidopsis
    Qing Jun Luo
    Department of Biological Sciences, Texas Tech University, Lubbock, Texas, USA
    PLoS Genet 5:e1000457. 2009
    ..HEN1 and SGS3 may be links for miRNA target entry into different RNA processing pathways...
  30. pmc Regulatory roles of natural antisense transcripts
    Mohammad Ali Faghihi
    Molecular and Integrative Neurosciences Department, The Scripps Research Institute, Jupiter, Florida 33458, USA
    Nat Rev Mol Cell Biol 10:637-43. 2009
    ..Recent evidence indicates that antisense transcripts are frequently functional and use diverse transcriptional and post-transcriptional gene regulatory mechanisms to carry out a wide variety of biological roles...
  31. pmc Antisense transcripts are targets for activating small RNAs
    Jacob C Schwartz
    Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, Texas 75390 9041, USA
    Nat Struct Mol Biol 15:842-8. 2008
    ..Our data demonstrate that antisense transcripts have a central role in recognition of the PR promoter by both activating and inhibitory agRNAs...
  32. ncbi Antisense RNA regulation of the par post-segregational killing system: structural analysis and mechanism of binding of the antisense RNA, RNAII and its target, RNAI
    T J Greenfield
    Division of Basic Biomedical Sciences, School of Medicine, University of South Dakota, Vermillion, SD 57069, USA
    Mol Microbiol 42:527-37. 2001
    The par stability determinant of the Enterococcus faecalis plasmid pAD1 is the first antisense RNA regulated post-segregational killing system (PSK) identified in a Gram-positive organism...
  33. pmc A novel antisense RNA regulates at transcriptional level the virulence gene icsA of Shigella flexneri
    Mara Giangrossi
    Laboratory of Molecular Genetics, Department of Biology MCA, University of Camerino, 62032 Camerino, MC, Italy
    Nucleic Acids Res 38:3362-75. 2010
    ..Within the intergenic region virA-icsA, we have discovered a new gene that encodes a non-translated antisense RNA (named RnaG), transcribed in cis on the complementary strand of icsA...
  34. ncbi The non-coding Air RNA is required for silencing autosomal imprinted genes
    Frank Sleutels
    Department of Molecular Genetics, The Netherlands Cancer Institute, Plesmanlaan 121, 1066CX, Amsterdam, The Netherlands
    Nature 415:810-3. 2002
    ..Our results indicate that non-coding RNAs have an active role in genomic imprinting...
  35. ncbi Mechanism of killer gene activation. Antisense RNA-dependent RNase III cleavage ensures rapid turn-over of the stable hok, srnB and pndA effector messenger RNAs
    K Gerdes
    Department of Molecular Biology, Odense University, Denmark
    J Mol Biol 226:637-49. 1992
    ..We show here, that both the presence of the antisense RNA and of the host enzyme RNase III is required for rapid cleavage of the truncated mRNAs, and we map the ..
  36. ncbi Mitotic phosphorylation of histone H3 is governed by Ipl1/aurora kinase and Glc7/PP1 phosphatase in budding yeast and nematodes
    J Y Hsu
    Department of Biochemistry and Molecular Genetics, University of Virginia, Charlottesville 22908, USA
    Cell 102:279-91. 2000
    ..Our findings reveal an enzyme system that regulates chromosome dynamics and controls histone phosphorylation that is conserved among diverse eukaryotes...
  37. pmc Integrative analysis of the human cis-antisense gene pairs, miRNAs and their transcription regulation patterns
    Oleg V Grinchuk
    Bioinformatics Institute, 30 Biopolis Street 07 01, Singapore 138672, Singapore
    Nucleic Acids Res 38:534-47. 2010
    ..The database is available from http://globalisland.bii.a-star.edu.sg/ approximately jiangtao/sas/index3.php?link =about...
  38. ncbi RNA: guiding gene silencing
    M Matzke
    Institute of Molecular Biology, Austrian Academy of Sciences, A 5020 Salzburg, Austria
    Science 293:1080-3. 2001
    ..RNA silencing is a potent means to counteract foreign sequences and could play an important role in plant and animal development...
  39. pmc Identification of TSIX, encoding an RNA antisense to human XIST, reveals differences from its murine counterpart: implications for X inactivation
    B R Migeon
    McKusick Nathans Institute of Genetic Medicine and Department of Pediatrics, The Johns Hopkins University School of Medicine, Baltimore, MD, 21287, USA
    Am J Hum Genet 69:951-60. 2001
    ..These differences could explain the fact that X inactivation is not imprinted in human placenta, and they raise questions about the role of TSIX in random X inactivation...
  40. pmc RNA-directed transcriptional gene silencing and activation in human cells
    Kevin V Morris
    Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, CA 92037, USA
    Oligonucleotides 19:299-306. 2009
    ....
  41. pmc A global view of the nonprotein-coding transcriptome in Plasmodium falciparum
    Carsten A Raabe
    Institute of Experimental Pathology, ZMBE, University of Muenster, Von Esmarch Str 56, 48149 Muenster, Germany
    Nucleic Acids Res 38:608-17. 2010
    ..falciparum, revealing an extended and sophisticated npcRNA network that may control the expression of housekeeping genes and virulence factors...
  42. pmc Defining a role for Hfq in Gram-positive bacteria: evidence for Hfq-dependent antisense regulation in Listeria monocytogenes
    Jesper Sejrup Nielsen
    Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, DK 5230 Odense M, Denmark
    Nucleic Acids Res 38:907-19. 2010
    ..Our findings indicate that modulation of translation by trans-encoded sRNAs may occur by both Hfq-dependent and -independent mechanisms, thus adding another layer of complexity to sRNA-mediated riboregulation in Gram-positive species...
  43. ncbi Distinct populations of primary and secondary effectors during RNAi in C. elegans
    Julia Pak
    Departments of Pathology and Genetics, Stanford University School of Medicine, Stanford, CA 94305 5324, USA
    Science 315:241-4. 2007
    ....
  44. ncbi Mechanism of post-segregational killing by the hok/sok system of plasmid R1: sok antisense RNA regulates formation of a hok mRNA species correlated with killing of plasmid-free cells
    K Gerdes
    Department of Molecular Biology, Odense University, Denmark
    Mol Microbiol 4:1807-18. 1990
    ..The expression of hok is regulated post-transcriptionally by the sok gene-encoded repressor, an antisense RNA complementary to the hok mRNA leader region...
  45. pmc Trans-natural antisense transcripts including noncoding RNAs in 10 species: implications for expression regulation
    Jiong Tang Li
    Center for Bioinformatics, National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Peking University, Beijing, 100871, PR China
    Nucleic Acids Res 36:4833-44. 2008
    ..In 30 cases, trans-SAs were related to cis-SAs through paralogues, suggesting a possible mechanism for the origin of trans-SAs. All trans-SAs are available at http://trans.cbi.pku.edu.cn/...
  46. pmc Molecular genetics and comparative genomics reveal RNAi is not functional in malaria parasites
    Jake Baum
    The Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria 3052, Australia
    Nucleic Acids Res 37:3788-98. 2009
    ....
  47. ncbi Rapid alteration of microRNA levels by histone deacetylase inhibition
    Gary K Scott
    Program of Cancer and Developmental Therapeutics, Buck Institute for Age Research, 8001 Redwood Boulevard, Novato, CA 94945, USA
    Cancer Res 66:1277-81. 2006
    ..These findings emphasize the importance of post-transcriptional mRNA regulation by HDACi in addition to their established effects on promoter-driven gene expression...
  48. pmc Genome-wide natural antisense transcription: coupling its regulation to its different regulatory mechanisms
    Michal Lapidot
    Department of Molecular Genetics, Weizmann Institute of Science, PO Box 26, Rehovot 76100, Israel
    EMBO Rep 7:1216-22. 2006
    ..According to this model, the observed relationship between the expression patterns of NATs and their targets might indicate the regulatory mechanism that is in action...
  49. pmc PU.1 expression is modulated by the balance of functional sense and antisense RNAs regulated by a shared cis-regulatory element
    Alexander K Ebralidze
    Harvard Institutes of Medicine, Harvard Stem Cell Institute, Harvard Medical School, Beth Israel Deaconess Medical Center, Boston, Massachusetts 02115, USA
    Genes Dev 22:2085-92. 2008
    ..We propose that such antisense RNAs will likely be important in the regulation of many genes and may be the reason for the large number of overlapping complementary transcripts with so far unknown function...
  50. pmc MicroRNA maturation: stepwise processing and subcellular localization
    Yoontae Lee
    Institute of Molecular Biology and Genetics and School of Biological Science, Seoul National University, Seoul 151 742, Korea
    EMBO J 21:4663-70. 2002
    ..Our study suggests that the regulation of miRNA expression may occur at multiple levels, including the two processing steps and the nuclear export step. These data will provide a framework for further studies on miRNA biogenesis...
  51. ncbi Detection of antisense RNA transcripts by strand-specific RT-PCR
    Eric C H Ho
    Department of Medical Biophysics, Sunnybrook Health Sciences Centre, University of Toronto, Toronto, ON, Canada
    Methods Mol Biol 630:125-38. 2010
    ..Results indicate that optimization of the screens requires proper controls to confirm removal of gDNA contamination and to rule out self-priming as a source of first strand products...
  52. ncbi Mechanism of post-segregational killing by the hok/sok system of plasmid R1. Sok antisense RNA regulates hok gene expression indirectly through the overlapping mok gene
    T Thisted
    Department of Molecular Biology, Odense University, Denmark
    J Mol Biol 223:41-54. 1992
    ..mediates plasmid stabilization by killing of plasmid-free segregants, codes for two RNAs, Hok mRNA and Sok antisense RNA. Hok mRNA encodes the Hok killer protein of 52 amino acid residues...
  53. pmc Genome-wide in silico identification and analysis of cis natural antisense transcripts (cis-NATs) in ten species
    Yong Zhang
    Center for Bioinformatics, National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Peking University Beijing, 100871, PR China
    Nucleic Acids Res 34:3465-75. 2006
    ..SA genes are over-represented in the catalytic activities and basic metabolism functions. All candidate cis-NATs can be downloaded from http://nats.cbi.pku.edu.cn/download/...
  54. pmc Regulated antisense RNA eliminates alpha-toxin virulence in Staphylococcus aureus infection
    Y Ji
    Department of Microbiology, SmithKline Beecham Pharmaceuticals Research and Development, Collegeville, Pennsylvania 19426, USA
    J Bacteriol 181:6585-90. 1999
    ..system for use in Staphylococcus aureus, with the goal of downregulating gene expression via induction of antisense RNA. We demonstrate that this system exhibits a 50- to 100-fold dose-dependent level of induction in bacterial ..
  55. pmc Evidence for a preferential targeting of 3'-UTRs by cis-encoded natural antisense transcripts
    Miao Sun
    Department of Medicine, Section of Hematology Oncology, University of Chicago, 5841 S Maryland Avenue, MC2115, Chicago, IL 60637, USA
    Nucleic Acids Res 33:5533-43. 2005
    ..These findings underscore the preference for, and conservation of, 3'-UTR-targeted antisense regulation, and the importance of 3'-UTRs in gene regulation...
  56. ncbi Variation of liver-type fatty acid binding protein content in the human hepatoma cell line HepG2 by peroxisome proliferators and antisense RNA affects the rate of fatty acid uptake
    C Wolfrum
    Department of Biochemistry, University of Munster, Wilhelm Klemm Str 2, D 48149, Munster, Germany
    Biochim Biophys Acta 1437:194-201. 1999
    ..55 to 0. 19 nmol/min per mg protein, i.e., by 66%. The analysis of peroxisome proliferator-treated cells and L-FABP mRNA antisense clones revealed a direct correlation between L-FABP content and fatty acid uptake...
  57. pmc Regulation of the large (approximately 1000 kb) imprinted murine Ube3a antisense transcript by alternative exons upstream of Snurf/Snrpn
    Miguel Landers
    Department of Genetics and Developmental Biology, University of Connecticut Health Center, Farmington, CT 06030, USA
    Nucleic Acids Res 32:3480-92. 2004
    ..We also show that the murine U exons have arisen by genomic duplication of segments that include elements of the IC, suggesting that the brain specific silencing of Ube3a is due to multiple alternatively spliced IC-Ube3a-ATS transcripts...
  58. ncbi Expression of antisense RNA to aldehyde dehydrogenase class-1 sensitizes tumor cells to 4-hydroperoxycyclophosphamide in vitro
    J S Moreb
    Division of Hematology Oncology, Department of Medicine, University of Florida, Gainesville, Florida32610 0277, USA
    J Pharmacol Exp Ther 293:390-6. 2000
    ..The present study examined the effect of ALDH-1 antisense RNA expression on ALDH-1 activity and sensitivity to 4-HC toxicity...
  59. ncbi As antisense RNA gets intronic
    Eduardo M Reis
    Department of Biochemistry, Institute of Chemistry, University of Sao Paulo, Sao Paulo, Brazil
    OMICS 9:2-12. 2005
    ....
  60. ncbi Role of endogenous antisense RNA in cardiac gene regulation
    Hans Peter Luther
    Medical Clinic I, Department of Cardiology, Humboldt University, Charite Hospital, Ziegelstrasse 5 9, 10117 Berlin, Germany
    J Mol Med (Berl) 83:26-32. 2005
    Endogenous antisense RNA has been detected for a range of eukaryotic genes and now appears to be a common phenomenon in mammalian cells...
  61. ncbi Neurons but not glial cells show reciprocal imprinting of sense and antisense transcripts of Ube3a
    K Yamasaki
    Department of Human Genetics, School of Medicine, Nagasaki University, 1 12 4 Sakamoto machi, Nagasaki, Japan
    Hum Mol Genet 12:837-47. 2003
    ..Reciprocal imprinting of sense and antisense transcripts present only in neurons suggests that the neuron-specific imprinting mechanism is related to the lineage determination of neural stem cells...
  62. pmc Small toxic proteins and the antisense RNAs that repress them
    Elizabeth M Fozo
    Cell Biology and Metabolism Program, Eunice Kennedy Shriver National Institute of Child Health and Human Development, 18 Library Drive, Bethesda, MD 20892 5430, USA
    Microbiol Mol Biol Rev 72:579-89, Table of Contents. 2008
    ....
  63. ncbi An mRNA amplification procedure with directional cDNA cloning and strand-specific cRNA synthesis for comprehensive gene expression analysis
    Satoko Ohtsuka
    Department of Biochemistry and Genetics, Chiba University Graduate School of Medicine, Chiba 260 8670, Japan
    Genomics 84:715-29. 2004
    ..The present system is widely applicable to gene expression analysis with limited amounts of RNA samples...
  64. pmc Dominant genetic screen for cofactors that enhance antisense RNA-mediated gene silencing in fission yeast
    Mitch Raponi
    Department of Biochemistry and Molecular Genetics, University of New South Wales, Sydney, NSW 2052, Australia
    Nucleic Acids Res 30:2546-54. 2002
    Specific gene silencing has been demonstrated in a number of organisms by the introduction of antisense RNA. Mutagenesis of host-encoded factors has begun to unravel the mechanism of several forms of RNA-mediated gene silencing and has ..
  65. pmc Precursor of C4 antisense RNA of bacteriophages P1 and P7 is a substrate for RNase P of Escherichia coli
    R K Hartmann
    Abteilung Erdmann, Freie Universitat Berlin, Germany
    Proc Natl Acad Sci U S A 92:5822-6. 1995
    ..C4 is an antisense RNA acting on a target, Ant mRNA, which is transcribed from the same promoter...
  66. ncbi Inhibition of basigin expression in glioblastoma cell line via antisense RNA reduces tumor cell invasion and angiogenesis
    Qinchuan Liang
    Department of Neurosurgery, Tangdu Hospital, Fourth Military Medical University, Xi an City, China
    Cancer Biol Ther 4:759-62. 2005
    ..In this study, we examined the inhibitory effects of antisense RNA of CD147 on invasion and angiogenesis of human glioblastoma U251 cells in vitro...
  67. ncbi Transcription of antisense RNA leading to gene silencing and methylation as a novel cause of human genetic disease
    Cristina Tufarelli
    MRC Molecular Haematology Unit, Weatherall Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital, Headington, Oxford OX3 9DS, UK
    Nat Genet 34:157-65. 2003
    ..the affected individual, in a transgenic model and in differentiating embryonic stem cells, transcription of antisense RNA mediates silencing and methylation of the associated CpG island...
  68. ncbi Development of an antisense RNA delivery system using conjugates of the MS2 bacteriophage capsids and HIV-1 TAT cell-penetrating peptide
    Baojun Wei
    National Center for Clinical Laboratories, Beijing Hospital, Beijing, People s Republic of China
    Biomed Pharmacother 63:313-8. 2009
    ..sulfo-SMPB), to conjugate the human immunodeficiency virus-1 (HIV-1) Tat peptide and MS2 VLPs; the antisense RNA against the 5'-untranslated region (UTR) and the internal ribosome entry site (IRES) of the hepatitis C virus (..
  69. ncbi SsrA (tmRNA) acts as an antisense RNA to regulate Staphylococcus aureus pigment synthesis by base pairing with crtMN mRNA
    Yu Liu
    Beijing Institute of Basic Medical Sciences, Beijing, China
    FEBS Lett 584:4325-9. 2010
    ..Furthermore, it was demonstrated that ssrA could act as an antisense RNA aside from its well-known biological function, and crtMN, encoding two essential enzymes for the pigment ..
  70. ncbi A non-muscle myosin required for embryonic polarity in Caenorhabditis elegans
    S Guo
    Section of Genetics and Development, Cornell University, Ithaca, New York 14853, USA
    Nature 382:455-8. 1996
    ..Furthermore, injections of nmy-2 antisense RNA into ovaries of adult worms cause embryonic partitioning defects and lead to mislocalization of PAR proteins...
  71. pmc Human Immunodeficiency Virus-Type 1 LTR DNA contains an intrinsic gene producing antisense RNA and protein products
    Linda B Ludwig
    Division of Allergy, Immunology and Rheumatology, Department of Medicine, School of Biomedical Science and Medicine, State University of New York at Buffalo, Buffalo, New York 14203, USA
    Retrovirology 3:80. 2006
    ..In this study we investigated the possibility of an antisense gene contained within the human immunodeficiency virus type 1 (HIV-1) long terminal repeat (LTR)...
  72. ncbi Transcription of the sulA-ompA region of Escherichia coli during the SOS response and the role of an antisense RNA molecule
    S T Cole
    Laboratoire de Génétique Moléculaire Bactérienne, Institut Pasteur, Paris, France
    Mol Microbiol 3:715-22. 1989
    ..However, the amount of OmpA protein produced was only slightly reduced...
  73. ncbi Can tRNAs act as antisense RNA? The case of mutA and dnaQ
    Robert Dorazi
    1 rue des Acacias, Jarny 54800, France
    J Theor Biol 225:383-8. 2003
    ..This could represent a new regulatory function for tRNAs and of course gives a direct and unrecognized link between starvation and mutation rate...
  74. pmc An antisense RNA-mediated transcriptional attenuation mechanism functions in Escherichia coli
    Sabine Brantl
    Institut fur Molekularbiologie, Friedrich Schiller Universitat Jena, Winzerlaer Strasse 10, Jena D 07745, Germany
    J Bacteriol 184:2740-7. 2002
    b>Antisense RNA-mediated transcriptional attenuation is a regulatory mechanism operating in the replication control of two groups of plasmids in gram-positive bacteria, the pT181 group and the inc18 family, represented by pIP501...
  75. pmc Antisense RNA-mediated suppression of benzophenanthridine alkaloid biosynthesis in transgenic cell cultures of California poppy
    Sang Un Park
    Department of Biological Sciences, University of Calgary, Calgary, Alberta, Canada T2N 1N4
    Plant Physiol 128:696-706. 2002
    ..The suppression of benzophenanthridine alkaloid biosynthesis using BBE or CYP80B1 antisense RNA constructs also reduced the growth rate of the cultures...
  76. ncbi Viral vector producing antisense RNA restores myotonic dystrophy myoblast functions
    D Furling
    Unit of Human Genetics, CHU Laval Research Center, Quebec, Canada
    Gene Ther 10:795-802. 2003
    ..We have produced a retrovirus which expresses a 149-bp antisense RNA complementary to the (CUG)13 repeats and to the 110-bp region following the repeats sequence to increase the ..
  77. ncbi Tsix, a gene antisense to Xist at the X-inactivation centre
    J T Lee
    Department of Molecular Biology, Massachusetts General Hospital, Harvard Medical School, Boston 02115, USA
    Nat Genet 21:400-4. 1999
    ..Tsix is not found on the inactive X once cells enter the X-inactivation pathway. Tsix has features suggesting a role in regulating the early steps of X inactivation, but not the silencing step...
  78. pmc Inhibition of HIV-1 multiplication by antisense U7 snRNAs and siRNAs targeting cyclophilin A
    Songkai Liu
    Institute of Cell Biology, University of Bern, Baltzerstrasse 4, 3012 Bern, Switzerland
    Nucleic Acids Res 32:3752-9. 2004
    ..Since the two types of antisense RNAs function by different mechanisms, combining the two approaches may result in a synergistic effect...
  79. ncbi Allelic IGF2R repression does not correlate with expression of antisense RNA in human extraembryonic tissues
    C B Oudejans
    Molecular Biology Laboratory, Department of Clinical Chemistry, University Hospital Vrije Universiteit, De Boelelaan 1117, Amsterdam, 1081 HV, The Netherlands
    Genomics 73:331-7. 2001
    ..This indicates that in contrast to the mouse, allelic IGF2R repression in the developing human placenta does not correlate with AIR expression...
  80. ncbi A genome-wide strategy for the identification of essential genes in Staphylococcus aureus
    R Allyn Forsyth
    Elitra Pharmaceuticals, San Diego, CA 92121, USA
    Mol Microbiol 43:1387-400. 2002
    ..a large number of essential genes for the bacterial pathogen, Staphylococcus aureus, using a rapid shotgun antisense RNA method...
  81. pmc miRNPs: a novel class of ribonucleoproteins containing numerous microRNAs
    Zissimos Mourelatos
    Howard Hughes Medical Institute, Department of Biochemistry and Biophysics, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104 6148, USA
    Genes Dev 16:720-8. 2002
    ..The genomic sequences predict that miRNAs are likely to be derived from larger precursors that have the capacity to form stem-loop structures...
  82. ncbi Inhibition of intravacuolar acidification by antisense RNA decreases osteoclast differentiation and bone resorption in vitro
    T Laitala-Leinonen
    Department of Anatomy, Institute of Biomedicine, University of Turku, Finland
    J Cell Sci 112:3657-66. 1999
    ..the transcription/translation of carbonic anhydrase II (CA II) and vacuolar H(+)-ATPase (V-ATPase) by antisense RNA molecules...
  83. ncbi Development of an integrative vector for the expression of antisense RNA in Clostridium difficile
    Adam P Roberts
    Division of Infection and Immunity, Eastman Dental Institute for Oral Health Care Sciences, University College London, University of London, 256 Gray s Inn Road, London WC1X 8LD, UK
    J Microbiol Methods 55:617-24. 2003
    ..This was used to introduce antisense RNA for the adhesin encoding gene cwp66 into C. difficile 79-685...
  84. ncbi An antisense transcript spanning the CGG repeat region of FMR1 is upregulated in premutation carriers but silenced in full mutation individuals
    Paula D Ladd
    Division of Human Biology, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA
    Hum Mol Genet 16:3174-87. 2007
    ..Moreover, it exhibits premutation-specific alternative splicing. Taken together, these observations suggest that in addition to FMR1, ASFMR1 may contribute to the variable phenotypes associated with the CGG repeat expansion...
  85. pmc Antisense RNA downregulation of coenzyme A transferase combined with alcohol-aldehyde dehydrogenase overexpression leads to predominantly alcohologenic Clostridium acetobutylicum fermentations
    Seshu B Tummala
    Department of Chemical Engineering, Northwestern University, Evanston, Illinois 60208, USA
    J Bacteriol 185:3644-53. 2003
    ..of the alcohol-aldehyde dehydrogenase (aad) gene and downregulation of the coenzyme A transferase (CoAT) using antisense RNA (asRNA) against ctfB (the second CoAT gene on the polycistronic aad-ctfA-ctfB message) was used in order to ..
  86. pmc Overexpression of a natural chloroplast-encoded antisense RNA in tobacco destabilizes 5S rRNA and retards plant growth
    Amber M Hotto
    Boyce Thompson Institute for Plant Research, Cornell University, Tower Rd, Ithaca, NY 14853, USA
    BMC Plant Biol 10:213. 2010
    ..In this study, we used Nicotiana tabacum to investigate the effect on sense strand transcripts of overexpressing a natural chloroplast asRNA, AS5, which is complementary to the region which encodes the 5S rRNA and tRNAArg...
  87. ncbi Degradation of FinP antisense RNA from F-like plasmids: the RNA-binding protein, FinO, protects FinP from ribonuclease E
    L J Jerome
    Department of Biological Sciences, University of Alberta, Edmonton, Alberta, T6G 2E9, Canada
    J Mol Biol 285:1457-73. 1999
    ..F FinP is a 79 base antisense RNA, composed of two stem-loops, complementary to the 5' untranslated leader of traJ mRNA...
  88. ncbi Effects of multidrug resistance, antisense RNA on the chemosensitivity of hepatocellular carcinoma cells
    Bo Li
    Department of Hepatobiliary Surgery, Second Affiliated Hospital, Chongqing University of Medical Sciences, Chongqing 400010, China
    Hepatobiliary Pancreat Dis Int 5:552-9. 2006
    Multidrug resistance is a major obstacle in cancer chemotherapy. We examined whether the antisense RNA of multidrug resistance gene 1 (mdr1) could reverse multidrug resistance in the human hepatocellular carcinoma (HCC) cell line ..
  89. ncbi Antisense RNA-mediated transcriptional attenuation: an in vitro study of plasmid pT181
    S Brantl
    Institut fur Molekularbiologie, Friedrich Schiller Universitat Jena, Winzerlaer Strasse 10, Jena D 07745, Germany
    Mol Microbiol 35:1469-82. 2000
    ..Binding rate constants for sense/antisense RNA pairs were measured, and functional segments required for complex formation were determined...
  90. ncbi The Prader-Willi syndrome imprinting center activates the paternally expressed murine Ube3a antisense transcript but represses paternal Ube3a
    S J Chamberlain
    Department of Molecular Genetics and Microbiology, University of Florida College of Medicine, Gainesville, Florida 32610, USA
    Genomics 73:316-22. 2001
    ....
  91. pmc An antisense RNA controls synthesis of an SOS-induced toxin evolved from an antitoxin
    Mitsuoki Kawano
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Mol Microbiol 64:738-54. 2007
    ..We suggest that SymE promotion of RNA cleavage may be important for the recycling of RNAs damaged under SOS-inducing conditions...
  92. pmc Comparative profiling of the sense and antisense transcriptome of maize lines
    Jiong Ma
    Department of Biological Sciences, Stanford University, Stanford, CA 94305 5020, USA
    Genome Biol 7:R22. 2006
    ..To determine the validity of comparisons among mutants in different lines, we first address the question of how similar the transcriptomes are in three standard lines at four developmental stages...
  93. pmc Bidirectional transcription directs both transcriptional gene activation and suppression in human cells
    Kevin V Morris
    Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, CA, USA
    PLoS Genet 4:e1000258. 2008
    ..suggest that in human cells, bidirectional transcription is an endogenous gene regulatory mechanism whereby an antisense RNA directs epigenetic regulatory complexes to a sense promoter, resulting in RNA-directed epigenetic gene ..
  94. ncbi Naturally occurring antisense RNA: function and mechanisms of action
    Andreas Werner
    RNA Biology Group, Institute for Cell and Molecular Biosciences, Newcastle University, Newcastle, UK
    Curr Opin Nephrol Hypertens 18:343-9. 2009
    ..The transcription of an antisense RNA can influence the output of the specific gene locus on a posttranscriptional level but may also help to ..
  95. pmc Distinct expression patterns of natural antisense transcripts in Arabidopsis
    Stefan R Henz
    Max Planck Institute for Developmental Biology, Department of Molecular Biology, Tuebingen, Germany
    Plant Physiol 144:1247-55. 2007
    ..Our results demonstrate that there is a trend toward anticorrelated expression of cis-NAT pairs in Arabidopsis, but currently available data do not produce a strong signature of small RNA-mediated silencing for this process...
  96. pmc Expression of the thyroid hormone receptor gene, erbAalpha, in B lymphocytes: alternative mRNA processing is independent of differentiation but correlates with antisense RNA levels
    M L Hastings
    Department of Biology, Marquette University, Milwaukee, WI 53233, USA
    Nucleic Acids Res 25:4296-300. 1997
    ..The correlation between RevErb and erbAalpha mRNA is consistent with negative regulation of alpha2 via antisense interactions with the complementary RevErb mRNA...
  97. ncbi fhlA repression by OxyS RNA: kissing complex formation at two sites results in a stable antisense-target RNA complex
    L Argaman
    Department of Molecular Genetics and Biotechnology, The Hebrew University Hadassah Medical School, Jerusalem, 91120, Israel
    J Mol Biol 300:1101-12. 2000
    ..Mutations at either site, which disrupt pairing of OxyS to fhlA, decrease the stability of this complex. Our results indicate that kissing complex formation is sufficient to repress fhlA translation by OxyS...
  98. pmc Expression profiles of multiple genes in single neurons of Alzheimer's disease
    N Chow
    Department of Neurobiology and Anatomy, University of Rochester, Rochester, NY 14642, USA
    Proc Natl Acad Sci U S A 95:9620-5. 1998
    ..We address this problem by using antisense RNA profiling of single cells...
  99. pmc Silencing of a gene adjacent to the breakpoint of a widespread Drosophila inversion by a transposon-induced antisense RNA
    Marta Puig
    Departament de Genetica i de Microbiologia, Universitat Autonoma de Barcelona, 08193 Bellaterra, Barcelona, Spain
    Proc Natl Acad Sci U S A 101:9013-8. 2004
    ..An antisense RNA that originates in the Foldback-like transposon Kepler inserted at the breakpoint junction in all of the 2j ..
  100. ncbi Functional analysis of cathepsin B-like cysteine proteases from Leishmania donovani complex. Evidence for the activation of latent transforming growth factor beta
    Ashwini Somanna
    Department of Biological Sciences, University of Calgary, Alberta T2N 1N4, Canada
    J Biol Chem 277:25305-12. 2002
    ..These results suggest that cathepsin B plays an important role in Leishmania survival within the host macrophages by activating latent TGF-beta1...
  101. ncbi Identification of critical staphylococcal genes using conditional phenotypes generated by antisense RNA
    Y Ji
    Department of Microbiology, Genetics Research, GlaxoSmithKline Pharmaceuticals Research and Development, Collegeville, PA 19426, USA
    Science 293:2266-9. 2001
    ..This genomic strategy offers an approach to the identification of staphylococcal gene products that could serve as targets for antibiotic discovery...

Research Grants66

  1. Small Regulatory RNAs of Coxiella burnetii - The Agent of Q Fever
    JAMES MICHAEL BATTISTI; Fiscal Year: 2013
    ..Moreover, the results will clarify the rol of 6S sRNA in g-proteobacteria, where orthologous s factors play distinctive roles in different bacterial species. ..
  2. Heterochromatin on the human inactive X chromosome
    Brian P Chadwick; Fiscal Year: 2012
    ..Meanwhile, antisense RNA originating from the Xi is sufficiently stable that it can be detected as a longer transcript...
  3. Genetically Specific Therapy Against Pathogenic Bacteria
    Stanley R Maloy; Fiscal Year: 2013
    ..bacteria would be infected by a synthetic virus, or virus-like particle, that brings into the bacterial cells antisense RNA. As the reader probably knows, one of the several uses of RNA in the cell is to regulate the expression of ..
  4. Epigenomics of SLE
    Kathleen E Sullivan; Fiscal Year: 2013
    ..Preliminary data demonstrate tissue-specific antisense RNA upstream of the TNF1 gene which is associated with binding of a transcriptional repressor...
  5. BP1 and Nuclear Hormone Signaling in Breast Cancer
    JOSEPH PINZONE; Fiscal Year: 2009
    ..2) Study the effect ofBPl overexpression and BP1 antisense RNA on estrogen receptor _ gene expression in breast cells...
  6. Non-coding RNAs for Epigenetic Transcriptional Silencing in Prostate Cancer
    Ming Ming Zhou; Fiscal Year: 2013
    ..of PRC1 for the INK4a/ARF locus requires CBX7 interactions with a non-coding RNA transcript ANRIL (the antisense RNA of INK4a locus) and H3K27me via its conserved chromodomain, highlighting a direct role of long non-coding RNA ..
  7. Identification and study of the vascular disease gene at 9p21.3
    Thomas Quertermous; Fiscal Year: 2013
    ..recent evidence now suggests that a cyclin-dependent kinase inhibitor gene, CDKN2B, and a non-coding antisense RNA in the CDKN2B locus named ANRIL, are the most likely candidate genes linked to the variation at 9p21.3...
  8. Global analysis of mRNA polarization in migrating endothelial cells
    Paul L Fox; Fiscal Year: 2010
    ..for modulating mRNA localization and cell migration by RNA-based approaches, for example, by treatment with antisense RNA oligomers targeting the specific RNA element...
  9. Regulation of the Na/K Pump by RNA Editing
    Joshua J C Rosenthal; Fiscal Year: 2013
    ..that we can trick human ADAR2 into editing human pumps by mimicking the squid secondary structure with an antisense RNA oligo. From the standpoint of public health, this work is significant on several fronts...
  10. Mechanism of UBE3A Imprint in Neurodevelopment
    WESTON THOMAS POWELL; Fiscal Year: 2013
    ..the paternal allele of UBE3A, and to characterize the localization of a paternally expressed long non-coding antisense RNA from this chromosome region during neurodevelopment for its potential role in the UBE3A imprint mechanism and ..
  11. Targeting the ETS Transcription Factor Rearrangement EWS/FLI in Ewing's Sarcoma
    Kimberly Stegmaier; Fiscal Year: 2013
    ..g., RNAi knock-down, antisense RNA) results in tumor cell apoptosis, cell cycle arrest, and loss of tumorigenicity...
  12. Epigenetic regulation of neuropathic pain: Role of native KV1.2 antisense RNA
    Yuan Xiang Tao; Fiscal Year: 2013
    ..The proposed studies will provide major conceptual advances to our understanding of the molecular mechanism of neuropathic pain and might open a door for developing new strategies for treating neuropathic pain. ..
  13. Antisense RNA Mediated Epigenetic Regulation of Brain Derived Neurotrophic Factor
    Mohammad Ali Faghihi; Fiscal Year: 2013
    ..Initially, we will study the mechanism of antisense RNA-mediated regulation of BDNF, in vitro, in primary cultures of human and mouse neuroepithelial cells...
  14. Molecular Switches: Regulatory RNAs Targeting Nuclear Hormone Receptor Promoters
    BETHANY ANN JANOWSKI; Fiscal Year: 2013
    ..Significance: Association between antisense RNA transcripts and small non-coding RNAs at promoters to control gene expression may represent a novel regulatory ..
  15. Restoration of Tumor Suppression Activity in Malignant Melanoma
    DAVID JOSEPH WEBER; Fiscal Year: 2013
    ..As a proof of principle for a drug design project, inhibiting S100B with small interfering antisense RNA (siRNAS100B) and several SBiXs was shown to restore wild-type p53 levels and its downstream gene products, as ..
  16. Neurotensin an endogenous neuroleptic-like peptide
    BECKY L KINKEAD; Fiscal Year: 2010
    ..Viral vectors expressing the antisense RNA to the NTT and NT2 receptors will also be generated in order to produce brain region specific knockdown of the ..
  17. HIV and ADAR Editing
    Marie Louise Hammarskjold; Fiscal Year: 2013
    ..Secondly, we will analyze ADAR editing of HIV sense/antisense RNA hybrids, using a therapeutic HIV antisense RNA as a model...
  18. The roles of Anaplasma phagocytophilum surface proteins in cellular invasion
    Jason A Carlyon; Fiscal Year: 2013
    ..phagocytophilum organisms that can be induced to express antisense RNA against ompA or asp14 to knock down OmpA or Asp14 expression...
  19. The roles of Anaplasma phagocytophilum surface proteins in cellular invasion
    Jason A Carlyon; Fiscal Year: 2012
    ..phagocytophilum organisms that can be induced to express antisense RNA against ompA or asp14 to knock down OmpA or Asp14 expression...
  20. PROCESSING AND FUNCTION OF POLYOMA RNA
    Gordon G Carmichael; Fiscal Year: 2013
    ..In the final aim we will carry out studies to learn more about how antisense RNA works in mammalian nuclei...
  21. The Antisense Transcriptome and the Epigenetics of Cancer.
    Peter K Vogt; Fiscal Year: 2013
    ..specific antisense transcripts will be determined by overexpression and by interference with the function of antisense RNA using RNAi constructs...
  22. Regulation of transcription termination and its link in mRNA surveillance
    Chi Ming Wong; Fiscal Year: 2013
    ..too late may disrupt the regulation of downstream genes in the same orientation and generate antisense RNA against genes in the opposite orientation. Either outcome would have significant impact on gene expression...
  23. INHIBITION OF NONERYTHROID SPECTRIN BY ANTISENSE RNA
    Randall Moon; Fiscal Year: 1993
    ..This will involve application of antisense RNA technology initially in Xenopus, but later in transgenic mice...
  24. GABA-A Receptor Gene Transfer to Prevent Epileptogenesis
    SHELLEY RUSSEK; Fiscal Year: 2004
    ..promoter driving the transcription of GABRA1 transgene to up-regulate alpha1 subunit levels, or a GABRA4 antisense RNA, to down-regulate alpha4 levels...
  25. RETROVIRAL GENE TRANSFER INTO PRIMARY ASTROCYTE
    MARION SHORT; Fiscal Year: 1992
    ..Gene expression of B-NGF will be determined by hybridization to radioactivity labelled antisense RNA probes using total cellular RNA, and poly A+RNA by Northern and dot blot analysis...
  26. FRACTIONATED HYPERTHERMIA AND HEAT SHOCK PROTEINS
    Gloria Li; Fiscal Year: 1999
    ..constitutively overexpress (i) human HSF1, (ii) human hsp7O, (iii) both human HSF1 and human hsp7O, and (iv) antisense RNA targeting the constitutive hsc7O and the heat-inducible hsp7O messages; and (b) Using these stable cell lines ..
  27. INTERFERENCE WITH STEROID RESPONSE GENES IN LEPIDOPTERA
    Lynn Riddiford; Fiscal Year: 2002
    ..We will also determine the role of the natural antisense RNA encoding a putative cuticle gene found in the 3' untranslated region of the ecdysone receptor gene...
  28. Target and antibiotic discovery in Bacillus anthracis
    Philip Youngman; Fiscal Year: 2002
    ..I of this proposal will be to develop the molecular biology tools for conditionally delivering random genomic antisense RNA fragments to B. anthracis cells. In Phase II a genome-wide screen in B...
  29. C/EDB BETA AND CHOP IN EPIDERMAL DIFFERENTIATION
    Edward Maytin; Fiscal Year: 2002
    ..doxycyline-inducible transgenes that encode both activators and inhibitors of C/EBPbeta and CHOP (including antisense RNA, and inhibitory protein isoforms of C/EBPs) will be studied in a calcium-dependent differentiation model in ..
  30. EDITING OF THYMIDYLATE SYNTHASE RNA
    BRUCE DOLNICK; Fiscal Year: 2002
    ..This laboratory discovered a gene (rTS) that generates a naturally occurring antisense RNA to TS RNA...
  31. TRANSGENIC IN HYPERTENSION--RAS RIBOZYNE INHIBITION
    John Mullins; Fiscal Year: 1992
    ..of the target mRNA by the use of synthetic catalytic RNA sequences (ribozymes) which are included within an antisense RNA construct...
  32. Ribonuclease III mechanism of double-strand RNA cleavage
    ALLEN NICHOLSON; Fiscal Year: 2003
    ..RNase III orthologues participate in mRNA and rRNA maturation, mRNA degradation, and antisense RNA action...
  33. MLK3 function in neurofibromatosis tumor cells
    John Kyriakis; Fiscal Year: 2009
    ..3. Accordingly, in Aim 1 we will use biochemical, pharmacologic, RNAi, morpholino antisense RNA and inducible cell lines to explore (i) if NF2 cell proliferation is ERK and/or JNK-dependent, (ii) if ..
  34. Self Complementary Recombinant AAV Vectors
    DOUGLAS MCCARTY; Fiscal Year: 2005
    ..These will be tested for the effects of the mutations on transcription initiation from the terminal repeats. ..
  35. Antisense inhibitors for enhanced plasmid production
    Jim Williams; Fiscal Year: 2005
    ..coli plasmid DNA can be more efficiently produced by co-expression of an antisense RNA targeting the ColEI origin represser RNA (RNAI)...
  36. REGULATION OF ACTOMYOSIN DEPENDENT FUCTIONS IN CELLS
    KRISTINE KAMM; Fiscal Year: 1999
    ..with adenovirus carrying cDNA sequences coding for 1) catalytically inactive MLCK (dominant negative), 2) antisense RNA to MLCK (enzyme depletion), 3) a non-phosphorylatable RLC (T18A/S19A), or 4) a combination of these...
  37. INHIBITION OF NONERYTHROID SPECTRIN BY ANTISENSE RNA
    Randall Moon; Fiscal Year: 1991
    ..Our approach is to use antisense RNA techniques to cause defects in alpha-fodrin cDNA in an antisense orientation under control of one of several ..
  38. HOXA 11 DEVELOPMENTAL FUNCTION
    S Potter; Fiscal Year: 1999
    ..In the developing limbs the expression of the antisense RNA is complementary to that of the sense RNA, with antisense transcripts appearing in abundance in domains where ..
  39. HUMAN MAMMARY CELL GROWTH AND FUNCTION IN DEFINED MEDIA
    RICHARD HAM; Fiscal Year: 1990
    ..focused on the development of research tools and needed for this research including the use of cloned cDNA and antisense RNA probes to analyze for human milk protein gene expression at the mRNA level, and antibodies to human milk ..
  40. MATERNAL MRNA LOCALIZATIONS IN A DETERMINATE EGG
    WILLIAM JEFFERY; Fiscal Year: 2002
    ..complementary 3 UTRs and may represent a novel means for controlling the extent of embryonic cell division by antisense RNA. Uro-1 is an ankrin repeat-containing (SHARK family) protein tyrosine kinase (PTK), which is localized in the ..
  41. IDENTIFICATION OF GENES THAT CONFER LPS SENSITIVITY
    Keiko Watanabe; Fiscal Year: 1999
    ..GSEs, which are short (150-500bp) cDNA fragments which encode dominantly acting peptides or inhibitory antisense RNA. Peptides will interact with, and block intracellular signalling pathways which result in cytotoxicity...
  42. RELATIONSHIP BETWEEN DNA DAMAGE AND CELLULAR SENESCENCE
    Lynn Harrison; Fiscal Year: 1999
    ..We are developing two antisense RNA expression vectors to decrease either mitochondrial or nuclear dUTPase...
  43. HIV CYTOPATHICITY--ANTISENSE RNA APPROACH
    Mario Stevenson; Fiscal Year: 1993
    ..Effectiveness of antisense RNA inhibition in restricting viral replication and cytopathicity in vitro may provide the rationale for the use ..
  44. mRNA expression by RPE cells in myopia
    Richard Stone; Fiscal Year: 2006
    ..We believe this program will lead to improved understanding of the etiology of refractive errors and ultimately to effective approaches to arrest myopia in children. ..
  45. TIP39KDA AND TOOTH FORMATION
    Michael Paine; Fiscal Year: 2007
    ..For this I will employ both an antisense RNA and a ribozyme approach...
  46. ANTISENSE RNA INHIBITION OF HIV VIA RETROVIRAL VECTORS
    Eli Gilboa; Fiscal Year: 1991
    The objective of the proposed studies is to investigate the use of antisense RNA inhibition as a method to render cells resistant to infection and replication of human immunodeficiency virus (HIV)...
  47. PROTO-ONCOGENE EIF-4E IN BREAST CANCER
    Arrigo De Benedetti; Fiscal Year: 2000
    ..This will be accomplished primarily with the use of expression vectors and antisense RNA technology, to either elevate or reduce the level of eIF-4E...
  48. REGULATION OF THE MAJOR OUTER MEMBRANE PORIN PROTEINS
    THOMAS SILHAVY; Fiscal Year: 2001
    ..MicF, an antisense RNA, prevents translation of the mRNA for the larger porin, OmpF, when certain toxic molecules such as bile salts ..
  49. PECAM-1 AND VASCULOGENESIS AND HEART DEVELOPMENT
    H Baldwin; Fiscal Year: 2001
    ..cells, b) PECAM-1 specific function blocking antibodies to perturb adhesion, and c) adenoviral mediated antisense RNA constructs to attenuate PECAM-1 expression...
  50. ANTISENSE INHIBITORS FOR TREATMENT OF MULTIPLE SCLEROSIS
    Duane Ruffner; Fiscal Year: 2002
    ..These studies will lead to the development of potent MS therapeutics in Phase II. l. Produce anti-MMP-9 antisense RNA and ribozyme libraries. 2. Use the libraries to identify effective anti~MMP-9 antisense RNA or ribozymes. 3...
  51. Molecular analysis of the genes involved in SCA8 ataxia
    MICHAEL KOOB; Fiscal Year: 2005
    ..abstract): Spinocerebellar ataxia type 8 (SCA8) is caused by a CTG expansion in an untranslated, endogenous antisense RNA that overlaps the Kelch-like 1 (KLHL1) gene...
  52. CLONING OF KAPPA-OPIOID RECEPTOR VIA CDNA EXPRESSION
    Ping Yee Law; Fiscal Year: 1993
    ..Deletion and nucleotide insertion mutation studies will be carried out to investigate the structural requirement for kappa-opioid receptor activities...
  53. ENHANCEMENT AND MODULATION OF ANTI-SENSE RNA ACTIVITY
    JONATHAN IZANT; Fiscal Year: 1992
    b>Antisense RNA is a valuable, though often unpredictable, strategy for experimental gene inhibition as well as a potential rational approach to genetic therapeutics...
  54. RETROVIRAL ANTI-SENSE RNA--CELLULAR AND VIRAL RESPONSES
    Edward Holmes; Fiscal Year: 1992
    ..retrovirus less susceptible to infection by this retrovirus? If so, what factors affect the extent of this resistance? 2) Do complementary cellular RNAs adversely affect packaging and release of viruses capable of infecting other cells?..
  55. TGFBeta receptor antagonists as anticancer agents
    Michael Reiss; Fiscal Year: 2004
    ..the use of TGFbeta neutralizing antibodies, of TGFbeta- binding proteins, such as decorin, and of TGFbeta1 antisense RNA oligonucleotides...
  56. REGULATION OF IS1O GENE EXPRESSION
    Robert Simons; Fiscal Year: 1993
    ..revealed many aspects of gene expression that are of general importance to molecular biologists, including antisense RNA control, the focus of much of the research efforts described here...
  57. GENE REGULATION AND INTERACTION -- NORMAL AND MALIGNANT
    HAROLD KLINGER; Fiscal Year: 1990
    ..If specific chromosomes are found to cosegregate with these phenotypes these hybrids could ultimately be used for isolating genes that impart these phenotypes to the hybrids...
  58. TUMORIGENESIS IN MICE LACKING OSTEOPONTIN
    Susan Rittling; Fiscal Year: 1999
    ..Others have shown that transformed cells expressing antisense RNA to OPN are impaired in their ability to grow in soft agar and to form tumors, so we expect to see a similar ..
  59. REGULATION OF OSTEOBLAST GENE EXPRESSION
    Marie Demay; Fiscal Year: 2007
    ..osteoblast differentiation, BIG-3 protein synthesis, in untransfected MC3T3-E 1 cells, will be blocked using antisense RNA strategies...
  60. Olfactory neurons from bipolar patients
    Chang Gyu Hahn; Fiscal Year: 2006
    ..1) ionic imaging and the neurobiology of the human ORN system and 2) Single cell antisense RNA amplification technique...
  61. RNA-RNA ANNEALING PROTEINS
    STEPHEN MUNROE; Fiscal Year: 1990
    ..During the course of experiments in which antisense RNA was used as a probe for pre-mRNA splicing, proteins present in nuclear extracts were observed to greatly ..
  62. NUCLEIC ACID STRUCTURE AND PROTEIN INTERACTIONS
    DONALD CROTHERS; Fiscal Year: 2001
    ..the nature of the complex between the loops of two small hairpin helices which serve as models for the sense-antisense RNA interaction that controls replication in ColE1 plasmid...
  63. REGULATABLE INHIBITION OF ANY GENE IN TRANSGENIC MICE
    Brian Johnston; Fiscal Year: 2000
    ..RNA Padlocks inactivate the expression of specific genes by forming a topological linkage between an antisense RNA and the target mRNA...
  64. Therapeutic Antisense RNA and The HIV Rev-RRE Pathway
    Marie Louise Hammarskjold; Fiscal Year: 2010
    ..evidence to suggest that efficient antisense inhibition is explained by (and dependent on) trafficking of the antisense RNA through the Rev/RRE pathway...
  65. HUMAN LEUKEMIC DIFFERENTIATION--ANTI-SENSE RNA STUDIES
    MARK TYKOCINSKI; Fiscal Year: 1992
    ....
  66. Directed Libraries of Self-Circularizing Antisense RNA
    SERGEI KAZAKOV; Fiscal Year: 2004
    ..They should find diverse applications, includinginhibitingthe expression of disease-related genes, functional genomics, and target validation. ..