phosphothreonine

Summary

Summary: The phosphoric acid ester of threonine. Used as an identifier in the analysis of peptides, proteins, and enzymes.

Top Publications

  1. ncbi Function of WW domains as phosphoserine- or phosphothreonine-binding modules
    P J Lu
    Cancer Biology Program, Division of Hematology Oncology, Department of Medicine, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, MA 02215, USA
    Science 283:1325-8. 1999
  2. ncbi The FHA domain
    Daniel Durocher
    Samuel Lumenfeld Research Institute, Mount Sinai Hospital, Toronto, ON, Canada M5G 1 X5
    FEBS Lett 513:58-66. 2002
  3. ncbi Autophosphorylation at Thr286 of the alpha calcium-calmodulin kinase II in LTP and learning
    K P Giese
    Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA
    Science 279:870-3. 1998
  4. ncbi Diphosphothreonine-specific interaction between an SQ/TQ cluster and an FHA domain in the Rad53-Dun1 kinase cascade
    Hyun Lee
    Genomics Research Center, Academia Sinica, Taipei 115, Taiwan
    Mol Cell 30:767-78. 2008
  5. pmc Structure of the CaMKIIdelta/calmodulin complex reveals the molecular mechanism of CaMKII kinase activation
    Peter Rellos
    University of Oxford, Nuffield Department of Clinical Medicine, Structural Genomics Consortium, Oxford, United Kingdom
    PLoS Biol 8:e1000426. 2010
  6. ncbi 1H NMR study on the binding of Pin1 Trp-Trp domain with phosphothreonine peptides
    R Wintjens
    Institut de Biologie de Lille, Institut Pasteur de Lille, CNRS UMR 8525, 1 rue du Professeur Calmette, BP 447, Lille 59021, France
    J Biol Chem 276:25150-6. 2001
  7. ncbi Structural insights into the enzymatic mechanism of the pathogenic MAPK phosphothreonine lyase
    Yongqun Zhu
    National Institute of Biological Sciences, Beijing, 102206, China
    Mol Cell 28:899-913. 2007
  8. pmc Hierarchical phosphorylation at N-terminal transformation-sensitive sites in c-Myc protein is regulated by mitogens and in mitosis
    B Lutterbach
    Department of Cell Biology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 2175
    Mol Cell Biol 14:5510-22. 1994
  9. ncbi Identification of the autophosphorylation sites of LRRK2
    Shogo Kamikawaji
    Department of Neuropathology and Neuroscience, Graduate School of Pharmaceutical Sciences, University of Tokyo, 7 3 1 Hongo, Bunkyoku, Tokyo, 113 0033 Japan
    Biochemistry 48:10963-75. 2009
  10. ncbi Phosphopeptide derivatization signatures to identify serine and threonine phosphorylated peptides by mass spectrometry
    M P Molloy
    Department of Biological Chemistry, University of Michigan Medical School, Ann Arbor 48109 0606, USA
    Anal Chem 73:5387-94. 2001

Detail Information

Publications213 found, 100 shown here

  1. ncbi Function of WW domains as phosphoserine- or phosphothreonine-binding modules
    P J Lu
    Cancer Biology Program, Division of Hematology Oncology, Department of Medicine, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, MA 02215, USA
    Science 283:1325-8. 1999
    ..Although phosphotyrosine-binding modules have been well-characterized, phosphoserine- or phosphothreonine-binding modules have not been described...
  2. ncbi The FHA domain
    Daniel Durocher
    Samuel Lumenfeld Research Institute, Mount Sinai Hospital, Toronto, ON, Canada M5G 1 X5
    FEBS Lett 513:58-66. 2002
    The forkhead-associated (FHA) domain is a small protein module recently shown to recognize phosphothreonine epitopes on proteins...
  3. ncbi Autophosphorylation at Thr286 of the alpha calcium-calmodulin kinase II in LTP and learning
    K P Giese
    Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA
    Science 279:870-3. 1998
    ..Thus, the autophosphorylation of alphaCaMKII at Thr286 appears to be required for LTP and learning...
  4. ncbi Diphosphothreonine-specific interaction between an SQ/TQ cluster and an FHA domain in the Rad53-Dun1 kinase cascade
    Hyun Lee
    Genomics Research Center, Academia Sinica, Taipei 115, Taiwan
    Mol Cell 30:767-78. 2008
    ..affinity for diphosphorylated relative to monophosphorylated Rad53-SCD1 due to the presence of two separate phosphothreonine-binding pockets...
  5. pmc Structure of the CaMKIIdelta/calmodulin complex reveals the molecular mechanism of CaMKII kinase activation
    Peter Rellos
    University of Oxford, Nuffield Department of Clinical Medicine, Structural Genomics Consortium, Oxford, United Kingdom
    PLoS Biol 8:e1000426. 2010
    ..The structural data, together with biophysical interaction studies, reveals the mechanism of CaMKII activation by calmodulin and explains many of the unique regulatory properties of these two essential signaling molecules...
  6. ncbi 1H NMR study on the binding of Pin1 Trp-Trp domain with phosphothreonine peptides
    R Wintjens
    Institut de Biologie de Lille, Institut Pasteur de Lille, CNRS UMR 8525, 1 rue du Professeur Calmette, BP 447, Lille 59021, France
    J Biol Chem 276:25150-6. 2001
    ....
  7. ncbi Structural insights into the enzymatic mechanism of the pathogenic MAPK phosphothreonine lyase
    Yongqun Zhu
    National Institute of Biological Sciences, Beijing, 102206, China
    Mol Cell 28:899-913. 2007
    The OspF family of phosphothreonine lyase, including SpvC from Salmonella, irreversibly inactivates the dual-phosphorylated host MAPKs (pT-X-pY) through beta elimination...
  8. pmc Hierarchical phosphorylation at N-terminal transformation-sensitive sites in c-Myc protein is regulated by mitogens and in mitosis
    B Lutterbach
    Department of Cell Biology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 2175
    Mol Cell Biol 14:5510-22. 1994
    ....
  9. ncbi Identification of the autophosphorylation sites of LRRK2
    Shogo Kamikawaji
    Department of Neuropathology and Neuroscience, Graduate School of Pharmaceutical Sciences, University of Tokyo, 7 3 1 Hongo, Bunkyoku, Tokyo, 113 0033 Japan
    Biochemistry 48:10963-75. 2009
    ..Further analysis of autophosphorylation will clarify the mechanism of activation of LRRK2, as well as the pathomechanism of PD in relation to overactivation of LRRK2...
  10. ncbi Phosphopeptide derivatization signatures to identify serine and threonine phosphorylated peptides by mass spectrometry
    M P Molloy
    Department of Biological Chemistry, University of Michigan Medical School, Ann Arbor 48109 0606, USA
    Anal Chem 73:5387-94. 2001
    ..The approach was further used to examine in vitro phosphorylation of recombinant human HSP22 by protein kinase C, revealing phosphorylation of Thr-63...
  11. ncbi Tandem mass spectrometry reveals that three photosystem II proteins of spinach chloroplasts contain N-acetyl-O-phosphothreonine at their NH2 termini
    H Michel
    Biology Department, Brookhaven National Laboratory, Upton, New York 11973
    J Biol Chem 263:1123-30. 1988
    ..The three peptides correspond to the NH2 termini of D1, D2, and CPa-2; and each begins with N-acetyl-O-phosphothreonine. Comparison with sequences deduced from cloned genes indicates that D1 and D2 have lost their initiating N-..
  12. ncbi Eliminylation: a post-translational modification catalyzed by phosphothreonine lyases
    Damian F Brennan
    Section of Structural Biology, The Institute of Cancer Research, Chester Beatty Laboratories, 237 Fulham Road, London, SW3 6JB, UK
    Trends Biochem Sci 34:108-14. 2009
    ..eliminylation, based on the recently delineated mechanism of the Shigella OspF and Salmonella SpvC phosphothreonine lyases...
  13. ncbi Mechanistic insights into the multistage gas-phase fragmentation behavior of phosphoserine- and phosphothreonine-containing peptides
    Amanda M Palumbo
    Department of Chemistry Michigan State University, East Lansing, Michigan 48824, USA
    J Proteome Res 7:771-9. 2008
    ..collision-induced dissociation (CID) behavior of a set of singly and doubly charged phosphoserine- and phosphothreonine-containing peptide ions, as well as their regioselectively or uniformly deuterated derivatives, in a ..
  14. ncbi Modelling of the gas-phase phosphate group loss and rearrangement in phosphorylated peptides
    Marko Rozman
    Laboratory for Chemical Kinetics and Atmospheric Chemistry, Ruđer Bošković Institute, Bijenicka 54, HR 10002, Zagreb, Croatia
    J Mass Spectrom 46:949-55. 2011
    ..The fragmentation dynamics of phosphoserine versus phosphothreonine containing peptides suggests that H(3)PO(4) neutral loss from phosphothreonine containing peptides is less ..
  15. ncbi Proteomic screen finds pSer/pThr-binding domain localizing Plk1 to mitotic substrates
    Andrew E H Elia
    Center for Cancer Research, Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Science 299:1228-31. 2003
    ..polo-box domain (PBD) of the mitotic kinase polo-like kinase 1 (Plk1) as a specific phosphoserine (pSer) or phosphothreonine (pThr) binding domain and determined its optimal binding motif...
  16. ncbi Structure and function of the phosphothreonine-specific FHA domain
    Anjali Mahajan
    Biophysics Program, Ohio State University, Columbus, OH 43210, USA
    Sci Signal 1:re12. 2008
    ..forkhead-associated (FHA) domain is the only known phosphoprotein-binding domain that specifically recognizes phosphothreonine (pThr) residues, distinguishing them from phosphoserine (pSer) residues...
  17. ncbi Activation of NMDA receptors induces dephosphorylation of DARPP-32 in rat striatal slices
    S Halpain
    Laboratory of Molecular and Cellular Neuroscience, Rockefeller University, New York, New York 10021
    Nature 343:369-72. 1990
    ....
  18. ncbi FHA domains as phospho-threonine binding modules in cell signaling
    Andrew Hammet
    St Vincent s Institute of Medical Research, Fitzroy, Victoria 3065, Australia
    IUBMB Life 55:23-7. 2003
    ....
  19. ncbi Bacterial FHA domains: neglected players in the phospho-threonine signalling game?
    Mark Pallen
    Division of Immunity and Infection, Birmingham University Medical School, UK
    Trends Microbiol 10:556-63. 2002
    ..The way is now open to identify the targets of each FHA domain, and their roles in cellular physiology, and perhaps even to develop novel FHA-blocking antibacterial agents...
  20. ncbi Structural basis for the catalytic mechanism of phosphothreonine lyase
    Linjie Chen
    Graduate Program in Chinese Academy of Medical Sciences and Beijing Union Medical College, Beijing 100730, China
    Nat Struct Mol Biol 15:101-2. 2008
    Salmonella SpvC belongs to a new enzyme family designated phosphothreonine lyases that irreversibly inactivate mitogen-activated protein kinases...
  21. ncbi Phosphothreonine-212 of Alzheimer abnormally hyperphosphorylated tau is a preferred substrate of protein phosphatase-1
    Abdur Rahman
    Department of Neurochemistry, New York Institute for Basic Research in Developmental Disabilities, 1050 Forest Hill Road, Staten Island, NY 10314, USA
    Neurochem Res 30:277-87. 2005
    ..Of the sites dephosphorylated, pT212 was only a substrate for PP1, as purified/enriched PP2A and PP2B from the same brains did not dephosphorylate this site...
  22. ncbi Chemical derivatization of phosphoserine and phosphothreonine containing peptides to increase sensitivity for MALDI-based analysis and for selectivity of MS/MS analysis
    Giorgio Arrigoni
    Department of Protein Technology, Lund University, Lund, Sweden
    Proteomics 6:757-66. 2006
    ..a simple one-step beta-elimination/Michael addition reaction for the derivatization of phosphoserine and phosphothreonine. The substitution of the negatively charged phosphate group by a positively charged S-ethylpyridyl group ..
  23. pmc The checkpoint kinase Hsl1p is activated by Elm1p-dependent phosphorylation
    Lee Szkotnicki
    Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, NC 27710, USA
    Mol Biol Cell 19:4675-86. 2008
    ..We identified elm1 mutants in a screen for defects in Swe1p degradation and show that a phosphomimic T273E mutation in HSL1 bypasses the need for Elm1p in this pathway...
  24. ncbi Suppression of survivin phosphorylation on Thr34 by flavopiridol enhances tumor cell apoptosis
    Nathan R Wall
    Department of Cancer Biology and Cancer Center, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
    Cancer Res 63:230-5. 2003
    ....
  25. pmc Multisite M-phase phosphorylation of Xenopus Wee1A
    Sun Young Kim
    Department of Molecular Pharmacology, Stanford University School of Medicine, Stanford, CA 94305 5174, USA
    Mol Cell Biol 25:10580-90. 2005
    ..These results also show that multisite phosphorylation cooperatively inactivates Wee1A and cooperatively promotes Wee1A proteolysis...
  26. ncbi Ca2+/calmodulin-dependent protein kinase II. Identification of a regulatory autophosphorylation site adjacent to the inhibitory and calmodulin-binding domains
    C M Schworer
    Howard Hughes Medical Institute, Vanderbilt University School of Medicine, Nashville, Tennessee 37232
    J Biol Chem 263:13486-9. 1988
    ..e. His-Arg-Gln-Glu-Thr(PO4)-. These findings indicate that autophosphorylation of Thr286 (alpha subunit) and Thr287 (beta subunit) is responsible for transition of CaM-kinase II to the Ca2+-independent form...
  27. ncbi Structural and functional analysis of phosphothreonine-dependent FHA domain interactions
    Simon Pennell
    Division of Molecular Structure, MRC National Institute for Medical Research, The Ridgeway, London NW71AA, UK
    Structure 18:1587-95. 2010
    ..Although they are unique in binding exclusively to phosphothreonine, the basis for this discrimination over phosphoserine has remained elusive...
  28. ncbi Solution structures of two FHA1-phosphothreonine peptide complexes provide insight into the structural basis of the ligand specificity of FHA1 from yeast Rad53
    C Yuan
    Department of Chemistry, The Ohio State University, Columbus OH 43210, USA
    J Mol Biol 314:563-75. 2001
    ..We suggest that differentiation between pT and pS-peptides by FHA1 can be attributed to hydrophobic interactions between the methyl group of the pT residue and the aliphatic protons of R83, S85, and T106 from FHA1...
  29. ncbi Serine-threonine protein kinase activity of Elm1p, a regulator of morphologic differentiation in Saccharomyces cerevisiae
    C M Koehler
    Department of Biochemistry and Biophysics, Iowa State University, Ames 50011, USA
    FEBS Lett 408:109-14. 1997
    ....
  30. pmc Multiple phosphoinositide 3-kinase-dependent steps in activation of protein kinase B
    Michael P Scheid
    Department of Experimental Therapeutics, University Health Network Department of Medical Biophysics, University of Toronto, Toronto, Ontario, Canada
    Mol Cell Biol 22:6247-60. 2002
    ....
  31. pmc A phosphothreonine residue at the C-terminal end of the plasma membrane H+-ATPase is protected by fusicoccin-induced 14-3-3 binding
    A Olsson
    Department of Plant Biochemistry, Lund University, P O Box 117, SE 221 00 Lund, Sweden
    Plant Physiol 118:551-5. 1998
    ..Turnover of the phosphate group of phosphothreonine-948 was inhibited by 14-3-3 binding, suggesting that this residue may form part of a binding motif for 14-3-3...
  32. ncbi Characterization of the intramolecular electron transfer pathway from 2-hydroxyphenazine to the heterodisulfide reductase from Methanosarcina thermophila
    E Murakami
    Department of Biochemistry, Beadle Center, University of Nebraska, Lincoln 68588 0664, USA
    J Biol Chem 276:2432-9. 2001
    ..We propose the following electron transfer pathway: HPhenH(2) to the high potential 4Fe cluster, to the low potential heme, and finally, to CoB-S-S-CoM...
  33. pmc SpvC is a Salmonella effector with phosphothreonine lyase activity on host mitogen-activated protein kinases
    Piotr Mazurkiewicz
    Centre for Molecular Microbiology and Infection, Imperial College London, Armstrong Road, London SW7 2AZ, UK
    Mol Microbiol 67:1371-83. 2008
    ..Using antibodies specific to phospho-amino acids and mass spectrometry we demonstrate that SpvC has phosphothreonine lyase activity on full-length phospho-Erk (pErk) and a synthetic 13-amino-acid phospho-peptide containing the ..
  34. ncbi A new approach to phosphoserine and phosphothreonine analysis in peptides and proteins: chemical modification, enrichment via solid-phase reversible binding, and analysis by mass spectrometry
    Florian Thaler
    Biology Department, Discovery Research Oncology, Pharmacia Corporation, Viale Pasteur 10, 20014 Nerviano, Italy
    Anal Bioanal Chem 376:366-73. 2003
    beta-Elimination of the phosphate group on phosphoserine and phosphothreonine residues and addition of an alkyldithiol is a useful tool for analysis of the phosphorylation states of proteins and peptides...
  35. ncbi Involvement of DARPP-32 phosphorylation in the stimulant action of caffeine
    Maria Lindskog
    Department of Neuroscience, Karolinska Institutet, 17177 Stockholm, Sweden
    Nature 418:774-8. 2002
    ..Together, these studies demonstrate the involvement of DARPP-32 and its phosphorylation/dephosphorylation in the stimulant action of caffeine...
  36. ncbi Phosphorylation of light-harvesting complex II and photosystem II core proteins shows different irradiance-dependent regulation in vivo. Application of phosphothreonine antibodies to analysis of thylakoid phosphoproteins
    E Rintamäki
    Department of Biology, University of Turku, FIN 20014 Turku, Finland
    J Biol Chem 272:30476-82. 1997
    An immunological approach using a polyclonal phosphothreonine antibody is introduced for the analysis of thylakoid protein phosphorylation in vivo...
  37. ncbi Generation and use of antibodies to phosphothreonine
    D Heffetz
    Methods Enzymol 201:44-53. 1991
  38. pmc The MPM-2 antibody inhibits mitogen-activated protein kinase activity by binding to an epitope containing phosphothreonine-183
    S Taagepera
    Department of Cell Biology, University of Virginia Health Sciences Center, Charlottesville 22908
    Mol Biol Cell 5:1243-51. 1994
    ..The MPM-2 phosphoepitope antibody recognizes an amino acid domain incorporating the regulatory phosphothreonine on activated p42mapk in eggs during meiosis and in mammalian cultured cells during the G0 to G1 transition.
  39. ncbi Kinase suppressor of ras is necessary for tumor necrosis factor alpha activation of extracellular signal-regulated kinase/mitogen-activated protein kinase in intestinal epithelial cells
    F Yan
    Department of Pediatrics, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
    Cancer Res 61:963-9. 2001
    ..We therefore conclude that KSR is an essential upstream regulator of TNF-alpha-stimulated ERK1/ERK2 activation, most likely mediated via direct phosphorylation of Raf-1...
  40. ncbi Zipper-interacting protein kinase induces Ca(2+)-free smooth muscle contraction via myosin light chain phosphorylation
    N Niiro
    Department of Physiology, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
    J Biol Chem 276:29567-74. 2001
    ..These results suggest that ZIP kinase is responsible for Ca(2+) independent myosin phosphorylation and contraction in smooth muscle...
  41. pmc Aurora B phosphorylates multiple sites on mitotic centromere-associated kinesin to spatially and temporally regulate its function
    Xin Zhang
    Department of Biology, Indiana University, Bloomington, IN 47405, USA
    Mol Biol Cell 18:3264-76. 2007
    ..Our study also shows that the interplay between multiple phosphorylation sites of MCAK may be critical to temporally and spatially control MCAK function...
  42. ncbi Phosphorylation of AZT-resistant human immunodeficiency virus type 1 reverse transcriptase by casein kinase II in vitro: effects on inhibitor sensitivity
    J B Lazaro
    Department BCMP, Harvard Medical School, Boston, Massachusetts, USA
    Biochem Biophys Res Commun 275:26-32. 2000
    ..These results suggest that phosphorylation of RT might be one factor involved in drug resistance in vivo...
  43. ncbi Reciprocal phosphorylation and regulation of endothelial nitric-oxide synthase in response to bradykinin stimulation
    M B Harris
    Vascular Biology Center, Department of Pediatrics, Medical College of Georgia, Augusta, Georgia 30912, USA
    J Biol Chem 276:16587-91. 2001
    ..These data suggest that BK activation of eNOS in BAECs primarily involves deinhibition of the enzyme through calcineurin-mediated dephosphorylation at Thr-497...
  44. ncbi Akt/PKB kinase phosphorylates separately Thr212 and Ser214 of tau protein in vitro
    Hanna Ksiezak-Reding
    Neuroinflammation Research Laboratories, Department of Psychiatry, Mount Sinai School of Medicine, Box 1230, One Gustave L Levy Place, New York, NY 10029, USA
    Biochim Biophys Acta 1639:159-68. 2003
    ..Our data suggest that phosphorylation of tau by Akt may play specific role(s) in Akt-mediated anti-apoptotic signaling, particularly relevant to AD and other neurodegenerations...
  45. pmc Activation of B-Raf kinase requires phosphorylation of the conserved residues Thr598 and Ser601
    B H Zhang
    Department of Biological Chemistry and Institute of Gerontology, University of Michigan Medical School, Ann Arbor, MI 48109 0606, USA
    EMBO J 19:5429-39. 2000
    ..Since Thr598 and Ser601 are conserved in all Raf family members from Caenorhabditis elegans to mammals, we propose that phosphorylation of these two residues may be a general mechanism for Raf activation...
  46. ncbi Protein kinase C-mediated desensitization of the neurokinin 1 receptor
    O Dery
    Departments of Surgery and Physiology, University of California San Francisco, San Francisco, California 94143 0660, USA
    Am J Physiol Cell Physiol 280:C1097-106. 2001
    ..Thus PKC regulates SP-induced Ca(2+) mobilization by full-length NK1-R and does not regulate a naturally occurring truncated variant. PKC does not mediate desensitization to repeated stimulation or endocytosis of the NK1-R...
  47. ncbi Phosphorylation of the pro-apoptotic protein BIK: mapping of phosphorylation sites and effect on apoptosis
    S Verma
    Institute for Molecular Virology, St. Louis University Health Sciences Center, St. Louis, MO 63110, USA
    J Biol Chem 276:4671-6. 2001
    ..Partial purification of the protein kinase from HeLa cell cytoplasmic extracts suggest that BIK may be phosphorylated by a casein kinase II-related enzyme...
  48. ncbi A multiplexed protein kinase assay
    Melissa D Shults
    Illumina Inc, San Diego, CA 92121 1975, USA
    Chembiochem 8:933-42. 2007
    ..After incubation, phosphoserine, phosphothreonine, and phosphotyrosine are chemically labeled, and the substrates are hybridized to a microarray with ..
  49. ncbi Method development and measurements of endogenous serine/threonine Akt phosphorylation using capillary electrophoresis for systems biology
    C V Suresh Babu
    Bioanalysis and Biotransformation Research Center, Korea Institute of Science and Technology, Cheongryang, Seoul, Korea
    Electrophoresis 26:3765-72. 2005
    ..These results highlight the feasibility of the CE method, which is a simple and reliable technique for determining and characterizing various enzyme reactions particularly kinase enzymes...
  50. ncbi Identification of phosphorylation sites in AMP-activated protein kinase (AMPK) for upstream AMPK kinases and study of their roles by site-directed mutagenesis
    Angela Woods
    Hormone and Metabolic Research Unit, Christian de Duve Institute of Cellular Pathology and Université de Louvain, Avenue Hippocrate 75, B 1200 Brussels, Belgium
    J Biol Chem 278:28434-42. 2003
    ..The newly identified phosphorylation sites could play a subtle role in the regulation of AMPK, e.g. in subcellular localization or substrate recognition...
  51. ncbi Growth hormone-induced phosphorylation of epidermal growth factor (EGF) receptor in 3T3-F442A cells. Modulation of EGF-induced trafficking and signaling
    Yao Huang
    Department of Medicine, Division of Endocrinology and Metabolism, University of Alabama at Birmingham, Birmingham, Alabama 35294, USA
    J Biol Chem 278:18902-13. 2003
    ..These data indicate that GH, by activating ERKs, can modulate EGF-induced EGFR trafficking and signaling and expand our understanding of mechanisms of cross-talk between the GH and EGF signaling systems...
  52. ncbi Phosphorylation of p27Kip1 at Thr-157 interferes with its association with importin alpha during G1 and prevents nuclear re-entry
    Incheol Shin
    Department of Cancer Biology and Medicine, Vanderbilt Ingram Comprehensive Cancer Center, Vanderbilt University School of Medicine, Nashville, TN 37232, USA
    J Biol Chem 280:6055-63. 2005
    ....
  53. pmc Stress- and mitogen-induced phosphorylation of the synapse-associated protein SAP90/PSD-95 by activation of SAPK3/p38gamma and ERK1/ERK2
    Guadalupe Sabio
    MRC Protein Phosphorylation Unit, School of Life Sciences, University of Dundee, Dundee DD1 5EH, UK
    Biochem J 380:19-30. 2004
    ....
  54. ncbi Akt mediates the cross-talk between beta-adrenergic and insulin receptors in neonatal cardiomyocytes
    Carmine Morisco
    Dipartimento di Medicina Clinica, Scienze Cardiovascolari ed Immunologiche, Universita Federico II, Napoli, Italy
    Circ Res 96:180-8. 2005
    ....
  55. ncbi Identifying protein kinase substrates: hunting for the organ-grinder's monkeys
    Daniel C Berwick
    Department of Pharmacology, Columbia University, 630 West 168th Street, New York, NY 10032, USA
    Trends Biochem Sci 29:227-32. 2004
  56. pmc A phosphoserine/threonine-binding pocket in AGC kinases and PDK1 mediates activation by hydrophobic motif phosphorylation
    Morten Frodin
    Department of Clinical Biochemistry, Glostrup Hospital, DK 2600 Glostrup, Denmark
    EMBO J 21:5396-407. 2002
    ..Here, we discover a pocket in the kinase domain of PDK1 that recognizes the phosphoserine/phosphothreonine in the hydrophobic motif by identifying two oppositely positioned arginine and lysine residues that bind the ..
  57. ncbi A mass spectrometry-based proteomic approach for identification of serine/threonine-phosphorylated proteins by enrichment with phospho-specific antibodies: identification of a novel protein, Frigg, as a protein kinase A substrate
    Mads Grønborg
    Department of Biochemistry and Molecular Biology, Center for Experimental Bioinformatics, University of Southern Denmark, Campusvej 55, 5230 Odense M, Denmark
    Mol Cell Proteomics 1:517-27. 2002
    ..In this report, we describe several antibodies that recognize phosphoserine/phosphothreonine-containing proteins by Western blotting...
  58. ncbi Another role for the celebrity: Akt and insulin resistance
    Rong Tian
    Circ Res 96:139-40. 2005
  59. pmc Identification and characterization of four novel phosphorylation sites (Ser31, Ser325, Thr336 and Thr366) on LKB1/STK11, the protein kinase mutated in Peutz-Jeghers cancer syndrome
    Gopal P Sapkota
    MRC Protein Phosphorylation Unit, MSI WTB complex, University of Dundee, Dow Street, Dundee DD1 5EH, Scotland, UK
    Biochem J 362:481-90. 2002
    ....
  60. ncbi Identification of insulin receptor substrate 1 serine/threonine phosphorylation sites using mass spectrometry analysis: regulatory role of serine 1223
    Moulun Luo
    School of Life Sciences, Arizona State University, Tempe, Arizona 85287 0701, USA
    Endocrinology 146:4410-6. 2005
    ..These data suggest that phosphorylation of Ser1223 dampens association of IRS-1 with SHP-2, thereby increasing net insulin-stimulated tyrosine phosphorylation...
  61. ncbi DNA damage induced hyperphosphorylation of replication protein A. 1. Identification of novel sites of phosphorylation in response to DNA damage
    Jonathan E Nuss
    Department of Biochemistry and Molecular Biology, Wright State University, School of Medicine, Dayton, Ohio 45435, USA
    Biochemistry 44:8428-37. 2005
    ....
  62. ncbi Defective Akt activation is associated with glucose- but not glucosamine-induced insulin resistance
    Bryce A Nelson
    Division of Endocrinology, Diabetes and Medical Genetics, Department of Medicine, Medical University of South Carolina, Charleston, South Carolina 29425, USA
    Am J Physiol Endocrinol Metab 282:E497-506. 2002
    ..Insulin resistance may ensue when PM-associated phospho-Akt decreases below a threshold. High glucose and glucosamine cause insulin resistance by different mechanisms in 3T3-L1 adipocytes...
  63. pmc Identification of phosphorylated residues that affect the activity of the mitotic kinase Aurora-A
    Laurie E Littlepage
    Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 99:15440-5. 2002
    ....
  64. ncbi The effects of intracellular calcium depletion on insulin signaling in 3T3-L1 adipocytes
    Dorothy Sears Worrall
    Department of Medicine, Division of Endocrinology and Metabolism, University of California, San Diego, La Jolla, California 92093, USA
    Mol Endocrinol 16:378-89. 2002
    ....
  65. ncbi BRCT repeats as phosphopeptide-binding modules involved in protein targeting
    Isaac A Manke
    Center for Cancer Research, Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Science 302:636-9. 2003
    ..domains in PTIP (Pax transactivation domain-interacting protein) and in BRCA1 as phosphoserine- or phosphothreonine-specific binding modules that recognize substrates phosphorylated by the kinases ATM (ataxia telangiectasia-..
  66. ncbi 14-3-3 proteins: a number of functions for a numbered protein
    Dave Bridges
    Life Sciences Institute, University of Michigan, 210 Washtenaw Avenue, Ann Arbor, MI 48109, USA
    Sci STKE 2005:re10. 2005
    ..are now known, 14-3-3s were the first proteins recognized to specifically bind a discrete phosphoserine or phosphothreonine motif...
  67. pmc Transmembrane modulator-dependent bacterial tyrosine kinase activates UDP-glucose dehydrogenases
    Ivan Mijakovic
    Laboratoire de génétique des microorganismes, CNRS INRA INA PG UMR2585, 78850 Thiverval Grignon, France
    EMBO J 22:4709-18. 2003
    ..YwqE, which is encoded by the fourth gene of the B.subtilis ywqCDEF operon, also dephosphorylates P-Tyr-YwqD...
  68. ncbi Neurofibrillary tangle-associated collapsin response mediator protein-2 (CRMP-2) is highly phosphorylated on Thr-509, Ser-518, and Ser-522
    Y Gu
    Department of Neuropathology, Faculty of Medicine, University of Tokyo, Hongo 7 3 1, Bunkyo ku, Tokyo 113 0033, Japan
    Biochemistry 39:4267-75. 2000
    ..The phosphorylation of this particular portion carboxyl-terminal to the basic region of CRMP-2 may play an important role in regulating its activity, and may be involved in the formation of degenerating neurites in AD brain...
  69. ncbi In vitro binding and phosphorylation of human immunodeficiency virus type 1 Nef protein by serine/threonine protein kinase
    M Bodeus
    INSERM U332, Institut Cochin de Genetique Moleculaire, université Paris V René Descartes, France
    J Gen Virol 76:1337-44. 1995
    ..The interaction of Nef with cellular kinases and its phosphorylation may be important in mediating the effects of Nef in HIV-1 pathogenesis...
  70. pmc Phospho.ELM: a database of phosphorylation sites--update 2008
    Francesca Diella
    Structural and Computational Biology Unit, European Molecular Biology Laboratory, 69117 Heidelberg, Germany
    Nucleic Acids Res 36:D240-4. 2008
    ..Phospho.ELM is available on line at: http://phospho.elm.eu.org...
  71. pmc Large-scale phosphorylation mapping reveals the extent of tyrosine phosphorylation in Arabidopsis
    Naoyuki Sugiyama
    Institute for Advanced Biosciences, Keio University, Tsuruoka, Japan
    Mol Syst Biol 4:193. 2008
    ..The distribution of phosphoserine, phosphothreonine, and phosphotyrosine sites was 85.0, 10.7, and 4.3%...
  72. ncbi Signaling through scaffold, anchoring, and adaptor proteins
    T Pawson
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario M5G 1X5, Canada
    Science 278:2075-80. 1997
    ..The role of scaffold, anchoring, and adaptor proteins that contribute to the specificity of signal transduction events by recruiting active enzymes into signaling networks or by placing enzymes close to their substrates is discussed...
  73. ncbi Mitogenic activation, phosphorylation, and nuclear translocation of protein kinase Bbeta
    R Meier
    Friedrich Miescher Institute, P O Box 2543, CH 4002 Basel, Switzerland
    J Biol Chem 272:30491-7. 1997
    ..These observations provide evidence that PKBbeta undergoes nuclear translocation upon mitogenic activation and support a role for PKB in signaling from receptor tyrosine kinases to the nucleus through phosphatidylinositol 3-kinase...
  74. ncbi CD10/neutral endopeptidase 24.11 is phosphorylated by casein kinase II and coassociates with other phosphoproteins including the lyn src-related kinase
    R K Ganju
    Department of Medicine, Dana Farber Cancer Institute, Harvard Medical School, Boston, MA 02115, USA
    Blood 88:4159-65. 1996
    ..Taken together, these data indicate that CD10/NEP is itself phosphorylated by CKII and that CD10/NEP co-associates with additional tyrosine phosphoproteins including lyn...
  75. ncbi The properties of the protein tyrosine phosphatase PTPMEG
    M Gu
    Washington University School of Medicine, Division of Hematology, St Louis, Missouri 63110, USA
    J Biol Chem 271:27751-9. 1996
    ..Prior incubation of platelets with calpeptin, an inhibitor of calpain, blocked the agonist-induced proteolysis...
  76. pmc Autophosphorylation sites participate in the activation of the double-stranded-RNA-activated protein kinase PKR
    D R Taylor
    Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA
    Mol Cell Biol 16:6295-302. 1996
    ..Esteban, Proc. Natl. Acad. Sci. USA 91:10551-10555, 1994), these data suggest that the central part of the PKR molecule, lying between its RNA-binding and catalytic domains, regulates kinase activity via autophosphorylation...
  77. pmc Identification of novel phosphorylation sites required for activation of MAPKAP kinase-2
    R Ben-Levy
    CRC Centre for Cell and Molecular Biology, Chester Beatty Laboratories, Institute for Cancer Research, London, UK
    EMBO J 14:5920-30. 1995
    ..The mutant Asp222/Asp334 was 20% as active as phosphorylated MAPKAP kinase-2, and this constitutively active form may be useful for studying its physiological roles...
  78. ncbi Purification and molecular cloning of Plx1, a Cdc25-regulatory kinase from Xenopus egg extracts
    A Kumagai
    Division of Biology, 216 76, Howard Hughes Medical Institute, California Institute of Technology, Pasadena, CA 91125, USA
    Science 273:1377-80. 1996
    ..Cdc25 phosphorylated by Plx1 reacted strongly with MPM-2, a monoclonal antibody to mitotic phosphoproteins. These studies indicate that Plx1 may participate in control of mitotic progression...
  79. ncbi Murine p53 is phosphorylated within the PAb421 epitope by protein kinase C in vitro, but not in vivo, even after stimulation with the phorbol ester o-tetradecanoylphorbol 13-acetate
    D M Milne
    Biomedical Research Centre, Ninewells Hospital and Medical School, University of Dundee, UK
    Oncogene 13:205-11. 1996
    ..The two-dimensional migration pattern of the novel group of peptides is consistent with phosphorylation of previously uncharacterised sites within the central DNA binding region of p53...
  80. pmc ATM kinase is a master switch for the Delta Np63 alpha phosphorylation/degradation in human head and neck squamous cell carcinoma cells upon DNA damage
    Yiping Huang
    Department of Dermatology, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21231, USA
    Cell Cycle 7:2846-55. 2008
    ..Our results implicate specific kinases, and target phosphorylation sites in the degradation of Delta Np63 alpha following DNA damage...
  81. pmc Posttranslational modifications of bovine osteopontin: identification of twenty-eight phosphorylation and three O-glycosylation sites
    E S Sørensen
    Protein Chemistry Laboratory, University of Aarhus, Denmark
    Protein Sci 4:2040-9. 1995
    ..Twenty-five phosphoserines and one phosphothreonine were located in Ser/Thr-X-Glu/Ser(P)/Asp motifs, and two phosphoserines were found in the sequence Ser-X-X-..
  82. ncbi Ca2+-independent smooth muscle contraction. a novel function for integrin-linked kinase
    J T Deng
    Smooth Muscle Research Group and Canadian Institutes of Health Research Group in Regulation of Vascular Contractility, Department of Biochemistry, University of Calgary Faculty of Medicine, Calgary, Alberta T2N 4N1, Canada
    J Biol Chem 276:16365-73. 2001
    ....
  83. pmc Purification of a murine protein-tyrosine/threonine kinase that phosphorylates and activates the Erk-1 gene product: relationship to the fission yeast byr1 gene product
    C M Crews
    Department of Cellular and Developmental Biology, Harvard University, Cambridge, MA 02138
    Proc Natl Acad Sci U S A 89:8205-9. 1992
    ..These data are discussed with regard to a possible signal transduction mechanism...
  84. pmc p40MO15 associates with a p36 subunit and requires both nuclear translocation and Thr176 phosphorylation to generate cdk-activating kinase activity in Xenopus oocytes
    J C Labbe
    CNRS INSERM, Montpellier, France
    EMBO J 13:5155-64. 1994
    ..Taken together, the above results identify protein-protein interactions, nuclear translocation and phosphorylation (by an unidentified kinase) as features of p40MO15 that are required for the generation of active CAK...
  85. ncbi Phosphorylation of casein kinase II by p34cdc2. Identification of phosphorylation sites using phosphorylation site mutants in vitro
    D G Bosc
    Manitoba Institute of Cell Biology, Manitoba Cancer Treatment and Research Foundation, Winnipeg, Canada
    J Biol Chem 270:25872-8. 1995
    ..These results indicate that Thr-344, Thr-360, Ser-362, and Ser-370 are the sites on the alpha subunit of casein kinase II that are phosphorylated in mitotic cells...
  86. ncbi Importance of autophosphorylation at Ser186 in the A-loop of salt inducible kinase 1 for its sustained kinase activity
    Yoshiko Katoh Hashimoto
    Laboratory of Cell Signaling and Metabolism, National Institute of Biomedical Innovation, Osaka 567 0085, Japan
    J Cell Biochem 104:1724-39. 2008
    ..This may also be the case for the other isoform SIK2, but not for SIK3...
  87. pmc GSK-3 beta targets Cdc25A for ubiquitin-mediated proteolysis, and GSK-3 beta inactivation correlates with Cdc25A overproduction in human cancers
    Tiebang Kang
    Howard Hughes Medical Institute, Washington University School of Medicine, St Louis, MO 63110, USA
    Cancer Cell 13:36-47. 2008
    ..Importantly, a strong correlation between Cdc25A overproduction and GSK-3beta inactivation was observed in human tumor tissues, indicating that GSK-3beta inactivation may account for Cdc25A overproduction in a subset of human tumors...
  88. pmc Heat-shock-induced activation of stress MAP kinase is regulated by threonine- and tyrosine-specific phosphatases
    A N Nguyen
    Section of Microbiology, University of California, Davis, California 95616, USA
    Genes Dev 13:1653-63. 1999
    ..Thus, transient activation of Spc1 upon heat shock is ensured by differential regulation of threonine and tyrosine phosphorylation...
  89. ncbi Casein kinase 2 binds and phosphorylates the nucleosome assembly protein-1 (NAP1) in Drosophila melanogaster
    M Li
    Department of Developmental Genetics, Heidelberg, Germany
    J Mol Biol 293:1067-84. 1999
    ..The location of CK2 phosphorylation sites in NAP1 suggests that their phosphorylation can contribute to a PEST-mediated protein degradation of NAP1 and the translocation of NAP1 between cytoplasm and nucleus...
  90. pmc Mitotic histone H3 phosphorylation by vaccinia-related kinase 1 in mammalian cells
    Tae Hong Kang
    Department of Life Science, Division of Molecular and Life Science, Pohang University of Science and Technology, San 31, Hyoja Dong, Pohang 790 784, Republic of Korea
    Mol Cell Biol 27:8533-46. 2007
    ..Overexpression of VRK1 resulted in a dramatic condensation of nuclei. Our findings collectively support a role of VRK1 as a novel mitotic histone H3 kinase in mammals...
  91. ncbi Rho-kinase phosphorylates eNOS at threonine 495 in endothelial cells
    Masayuki Sugimoto
    Department of Vascular Surgery, Graduate School of Medicine, Nagoya University, 65 Tsurumai, Showa Ku, Nagoya, Aichi 466 8550, Japan
    Biochem Biophys Res Commun 361:462-7. 2007
    ..Treatment with Y27632, a Rho-kinase inhibitor, suppressed thrombin-induced phosphorylation at Thr495. These results indicate that Rho-kinase can directly phosphorylate eNOS at Thr495 to suppress NO production in endothelium...
  92. ncbi Phosphorylation of the transcription factor forkhead family member FKHR by protein kinase B
    G Rena
    Department of Biochemistry, Medical Research Council Protein Phosphorylation Unit, University of Dundee, Dundee DD1 5EH, Scotland, United Kingdom
    J Biol Chem 274:17179-83. 1999
    ..The conservation of Thr-24, Ser-256, and Ser-319 and the sequences surrounding them in DAF16 therefore suggests that DAF16 is also a direct substrate for PKB in C. elegans...
  93. ncbi Regulation of zipper-interacting protein kinase activity in vitro and in vivo by multisite phosphorylation
    Paul R Graves
    Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Biol Chem 280:9363-74. 2005
    ..Together, our findings show that ZIPK is positively regulated by phosphorylation within its kinase domain and that it contains an inhibitory C-terminal domain that controls enzyme activity, localization, and oligomerization...
  94. ncbi GSK-3-mediated phosphorylation enhances Maf-transforming activity
    Nathalie Rocques
    Institut Curie, Centre de Recherche, Orsay F 91405, France
    Mol Cell 28:584-97. 2007
    ..We further demonstrate that P/CAF protects MafA from ubiquitination and degradation, suggesting that, upon the release of the coactivator complex, MafA becomes polyubiquitinated and degraded to allow the response to terminate...
  95. ncbi PDK1 acquires PDK2 activity in the presence of a synthetic peptide derived from the carboxyl terminus of PRK2
    A Balendran
    MRC Protein Phosphorylation Unit, Department of Biochemistry, University of Dundee, Dundee DD1 5EH, UK
    Curr Biol 9:393-404. 1999
    ..Thr308 is phosphorylated by the 3-phosphoinositide-dependent protein kinase-1 (PDK1) but the identity of the kinase that phosphorylates Ser473 (provisionally termed PDK2) is unknown...
  96. ncbi Correlations in palmitoylation and multiple phosphorylation of rat bradykinin B2 receptor in Chinese hamster ovary cells
    V Soskic
    Institute of Molecular Biotechnology e V, 07745 Jena, Germany
    J Biol Chem 274:8539-45. 1999
    ....
  97. pmc Lyn is activated during late G1 of stem-cell-factor-induced cell cycle progression in haemopoietic cells
    S Mou
    Intramural Research and Support Program, SAIC Frederick, National Cancer Institute Frederick Cancer Research and Development Center, Frederick, MD 21702, USA
    Biochem J 342:163-70. 1999
    ..These findings demonstrate that SCF activates the Src family member Lyn before the G(1)/S transition of the cell cycle and suggest that Lyn is involved in SCF-induced cell cycle progression...
  98. ncbi Signaling through transforming G protein-coupled receptors in NIH 3T3 cells involves c-Raf activation. Evidence for a protein kinase C-independent pathway
    P Crespo
    Molecular Signaling Unit, Laboratory of Cellular Development and Oncology, NIDR, National Institutes of Health, Bethesda, Maryland 20892
    J Biol Chem 269:21103-9. 1994
    ..Phosphoamino acid analysis of slower mobility forms of p72Raf-1 revealed both phosphoserine and phosphothreonine. Carbachol potently induced c-Raf activity as judged by its in vitro phosphorylating activity using MEK as a ..
  99. ncbi Regulation of the human bradykinin B2 receptor expressed in sf21 insect cells: a possible role for tyrosine kinases
    G Reyes-Cruz
    Instituto de Fisiologia Celular, Universidad Nacional Autonoma de Mexico, Mexico, D F 04510 Mexico
    J Cell Biochem 76:658-73. 2000
    ..Phosphoaminoacid analysis revealed the presence of phosphoserine and traces of phosphothreonine, but not phosphotyrosine, suggesting that the putative tyrosine kinase(s), activated by bradykinin, could act ..
  100. ncbi Paxillin interactions
    C E Turner
    Dept of Cell and Developmental Biology, SUNY Upstate Medical University, Syracuse, NY13210, USA
    J Cell Sci 113:4139-40. 2000
    ..Others, such as the E6 protein from Papillomavirus, facilitate transformation by disrupting the normal links between paxillin and the actin cytoskeleton by displacing paxillin-LD-motif-binding proteins...
  101. ncbi Protein-tyrosine kinase activity and pp60v-src expression in whole cells measured by flow cytometry
    P N Preis
    Brain Tumor Research Center, School of Medicine, University of California, San Francisco 94143
    Cancer Res 48:4633-8. 1988
    ..specific; it was entirely inhibited by adding excess phenylphosphate but was not affected by phosphoserine or phosphothreonine. The relationship between the amount of phosphorylated tyrosine measured by our FCM technique and total ..

Research Grants40

  1. Mechanisms of Lipid-Induced Hepatic Insulin Resistance
    Gerald I Shulman; Fiscal Year: 2013
    ..IRK activity, utilizing state-of-the-art LC-MS/MS methods to identify novel PKC5-induced IRK phosphothreonine sites...
  2. Chemistry and biology of protein histidine phosphorylation
    Tom Muir; Fiscal Year: 2013
    ..While protein phosphorylation is known to occur on several amino acids, it is fair to say that phosphoserine, phosphothreonine and phosphotyrosine capture most of the attention in the literature, at least as it pertains to cellular ..
  3. Aptamers to distinguish functional modification of target proteins by affinity pr
    STEVEN SULJAK; Fiscal Year: 2009
    ..APCE methods for a selected peptide domain, specific modified peptides that incorporate phosphoserine and/or phosphothreonine will be investigated as models of typical post-translational modifications...
  4. MOLECULAR MECHANISM OF MATRIX PROTEIN PHOSPHORYLATION
    CHOU WU; Fiscal Year: 1993
    ..The preferential phosphoamino acid residue (phosphoserine or phosphothreonine) and the sequence specificity among the sites of the phosphorylated substrates will also be determined...
  5. SPECIFICITY OF PROTEIN TYROSINE PHOSPHATASES
    Zhong Yin Zhang; Fiscal Year: 2001
    ..dual specificity phosphatases that can utilize substrates containing phosphotyrosine as well as phosphoserine/phosphothreonine. Although ligand binding and localization/targeting domains are believed to be involved in the regulation of ..
  6. STRUCTURE/FUNCTION ANALYSIS OF MITOTIC REGULATORS
    JOSEPH NOEL; Fiscal Year: 2001
    ..Structural work in progress, confirms that optimal interaction with this basic cluster is achieved with phosphothreonine or phosphoserine containing substrates...
  7. The Function of Dual Specificity Phosphatase-5 in Immune Response
    Yusen Liu; Fiscal Year: 2009
    ..kinase (MAPK) phosphatases (MKPs) family, which can inactivate MAPKs by removing the phosphates from both the phosphothreonine and phosphotyrosine residues...
  8. STRUCTURE/FUNCTION OF THE MAPK PHOSPHATASE--PAC1
    Ming Ming Zhou; Fiscal Year: 2003
    ..3. Investigate the catalytic mechanisms for phosphotyrosine and phosphothreonine hydrolysis by the PAC1 phosphatase, using NMR spectroscopy, mutagenesis, and enzyme kinetic studies.
  9. REGULATION OF ARNT FUNCTION BY PHOSPHORYLATION STATUS
    Steven Levine; Fiscal Year: 1999
    ..phosphorylation sites will be examined by following these aims: 1) Produce a phosphopeptide map for ARNT's phosphothreonine sites and identify the location of the phosphothreonine residues with the highest stoichiometry; 2) Assess ..
  10. ISOLATION OF INSULIN RECEPTOR PHOSPHATASES
    Laurel Sweet; Fiscal Year: 1991
    ..be examined comparing dephosphorylation of the insulin receptor to other phosphotyrosine, phosphoserine, and phosphothreonine-containing substrates...
  11. 2004 Growth Factor Signalling Gordon Conference
    Michael Yaffe; Fiscal Year: 2008
    ..abstract_text> ..
  12. Identification of Molecular Pathways in Cancer Biology
    Michael Yaffe; Fiscal Year: 2005
    ..abstract_text> ..
  13. High-Throughput Assay Design:Polo Kinase Inhibitors(RMI)
    Michael Yaffe; Fiscal Year: 2004
    ..The development of these assays should allow rapid screening of small molecule Polo kinase inhibitors that will function as novel anti-cancer agents. ..
  14. NCRR Shared Instrumentation Grant (SIG) Program
    Michael Yaffe; Fiscal Year: 2005
    ..abstract_text> ..
  15. Phosphoserine Dependant Assembly of Signaling complexes
    Michael Yaffe; Fiscal Year: 2007
    ..unreadable] [unreadable]..
  16. Function and Regulation of the Phosphorylation-Specific Prolyl lsomerase Pin 1
    Kun Ping Lu; Fiscal Year: 2010
    ..In lay language, we have identified a new enzyme called Pin1 critical for cancer development and will continue to investigate the function and regulation of Pin1 during cancer development and hope to identify new drug targets. ..
  17. Whole cell and essential enzyme screening assays for mycothiol biosynthesis
    ROBERT FAHEY; Fiscal Year: 2009
    ..These HTS assays are designed to meet the goals of PA-04-068 and will be proposed for screening of libraries available under the Molecular Libraries Initiative. ..
  18. The roles of MKP-1 in Gram-negative bacterial sepsis and colitis
    Yusen Liu; Fiscal Year: 2010
    ..These studies will uncover critical immune regulatory mechanisms, and may lead to novel strategies for the treatment of sepsis and inflammatory bowel disease. ..
  19. Advancing High Throughput Flow Cytometry
    Bruce S Edwards; Fiscal Year: 2010
    ....
  20. Substrate specific ERK docking domain inhibitors
    Paul S Shapiro; Fiscal Year: 2010
    ..These compounds will be appropriate for research tools and the development into novel chemotherapeutic agents aimed at treating cancers associated with elevated ERK pathway activity. ..
  21. Response of Rhesus Sperm to Freezing: Recovery from Osmotic and Oxidative Stress
    Stuart A Meyers; Fiscal Year: 2010
    ....
  22. IKKBeta Mediated Impairment of Endothelial Nitric Oxide Production
    Francis Kim; Fiscal Year: 2010
    ..Aim 3. To determine whether the Toll Like Receptor pathway is necessary for endothelial activation of IKKbeta during the development of vascular insulin resistance. ..
  23. The Role of MKP-1 in innate immune responses to LPS
    Yusen Liu; Fiscal Year: 2010
    ..More importantly, these studies may reveal novel therapeutic targets for treating sepsis and sepsis-associated shock and multiple organ failure syndrome. ..
  24. Prolyl Isomerase Pin 1 and Neurodegeneration
    Kun Ping Lu; Fiscal Year: 2009
    ..These studies should help elucidate the molecular mechanisms of AD and related disorders, and may also have novel implications for their therapies. ..
  25. Protein Prenyltransferases in Glucose-Stimulated Insulin Secretion
    Anjaneyulu Kowluru; Fiscal Year: 2010
    ....
  26. PLASMID-MEDIATED VIRULENCE IN SALMONELLA
    Donald Guiney; Fiscal Year: 2008
    ..unreadable] [unreadable]..
  27. Physiological Roles of Receptor-Associated Immunophilins
    MARC COX; Fiscal Year: 2007
    ..Thus, these studies could also contribute drugs to a better understanding of the mechanism by which patients acquire resistance to corticosteroid drugs. [unreadable] [unreadable]..
  28. INTERACTIONS OF HELICOBACTER WITH THE GASTRIC MUCOSA
    Donald Guiney; Fiscal Year: 2001
    ..Finally, iNOS, IL-4, IL-10 and IFN-g knockout mice will also be used to determine the importance of these enzymes and cytokines in H. pylon-induced gastritis. ..
  29. CYCLIC GMP PHOSPHODIESTERASES IN PC12 DIFFERENTIATION
    John Bentley; Fiscal Year: 2002
    ....
  30. Binding Network of Pol Eta in DNA Damage Responses
    Christoph Borchers; Fiscal Year: 2003
    ..abstract_text> ..
  31. ROLE OF PROTEIN PHOSPHATASES IN INSULIN SECRETION
    Anjaneyulu Kowluru; Fiscal Year: 2004
    ....
  32. IDENTIFICATION AND STUDY OF PPARgamma-REGULATED GENES
    DOROTHY SEARS; Fiscal Year: 2004
    ..abstract_text> ..
  33. A NOVEL APPROACH TO GERM-LIINE PRESERVATION IN MACAQUES
    Stuart Meyers; Fiscal Year: 2004
    ..Dobrinski and experiments involving germ cell transplantation into male macaques will be performed at UC Davis. We expect to expand this study into a fully-independent project. ..
  34. Development of ERK docking domain inhibitors
    Paul Shapiro; Fiscal Year: 2005
    ..These studies represent a novel approach for developing compounds that selectively block ERK activation of proteins involved in cancer cell proliferation. ..
  35. INSULIN RECEPTOR SUBSTRATE -1 AND FLUID SHEAR STRESS
    Francis Kim; Fiscal Year: 2005
    ..To further characterize this potential link between insulin and NO signaling. Specific Aim 3: Characterize FSS dependent signaling elements which associate with IRS-1...
  36. Function and Regulation of Pin2/TRF1 and PinX1
    Kun Ping Lu; Fiscal Year: 2005
    ..These studies should yield new insights into telomere maintenance, cell growth control and oncogenesis, and may lead to the development of new anticancer therapy. ..
  37. Apoptosis in Sepsis: Regulation by TNF & delta-PKC
    Laurie Kilpatrick; Fiscal Year: 2006
    ..4: Assess the role of delta-PKC in regulating the assembly of TNFalpha anti-apoptotic signaling complexes and their association with the p60TNFR. ..
  38. Salmonella-to-Eukaryotic Cell Multi-task Gene Delivery
    Donald Guiney; Fiscal Year: 2006
    ..These studies are intended to develop attenuated Salmonella strains for DNA delivery for DNA vaccine approaches, corrective gene therapy, immunotherapy, and the prevention and treatment of certain kinds of cancer. ..
  39. MYCOTHIOL BIOSYNTHESIS AND METABOLISM AS TB DRUG TARGETS
    ROBERT FAHEY; Fiscal Year: 2006
    ..abstract_text> ..
  40. Assay for Formylpeptide Receptor Family Ligands(RMI)
    Bruce Edwards; Fiscal Year: 2005
    ..We expect that such compounds will prove to be important tools with which to advance the understanding and therapy of inflammation-associated diseases. ..