lysine

Summary

Summary: An essential amino acid. It is often added to animal feed.

Top Publications

  1. doi Lysine acetylation targets protein complexes and co-regulates major cellular functions
    Chunaram Choudhary
    Proteomics and Signal Transduction, Max Planck Institute for Biochemistry, Martinsried, Germany
    Science 325:834-40. 2009
  2. pmc Molecular interplay of the noncoding RNA ANRIL and methylated histone H3 lysine 27 by polycomb CBX7 in transcriptional silencing of INK4a
    Kyoko L Yap
    Department of Structural and Chemical Biology, Mount Sinai School of Medicine, New York, NY 10029, USA
    Mol Cell 38:662-74. 2010
  3. doi Quantitative interaction proteomics and genome-wide profiling of epigenetic histone marks and their readers
    Michiel Vermeulen
    Department of Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, D 82152 Martinsried, Germany
    Cell 142:967-80. 2010
  4. ncbi Loss of acetylation at Lys16 and trimethylation at Lys20 of histone H4 is a common hallmark of human cancer
    Mario F Fraga
    Cancer Epigenetics Laboratory, Molecular Pathology Program, Spanish National Cancer Center, Melchor Fernandez Almagro 3, 28029 Madrid, Spain
    Nat Genet 37:391-400. 2005
  5. ncbi Histone methyltransferase activity of a Drosophila Polycomb group repressor complex
    Jurg Muller
    EMBL, Gene Expression Programme, Meyerhofstr 1, 69117 Heidelberg, Germany
    Cell 111:197-208. 2002
  6. ncbi Methylation of lysine 4 on histone H3: intricacy of writing and reading a single epigenetic mark
    Alexander J Ruthenburg
    Laboratory of Chromatin Biology, The Rockefeller University, New York, NY 10021, USA
    Mol Cell 25:15-30. 2007
  7. ncbi The diverse functions of histone lysine methylation
    Cyrus Martin
    Howard Hughes Medical Institute, Department of Biochemistry and Biophysics, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, North Carolina 27599 7295, USA
    Nat Rev Mol Cell Biol 6:838-49. 2005
  8. ncbi Histone H4-K16 acetylation controls chromatin structure and protein interactions
    Michael Shogren-Knaak
    Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Science 311:844-7. 2006
  9. ncbi Drosophila enhancer of Zeste/ESC complexes have a histone H3 methyltransferase activity that marks chromosomal Polycomb sites
    Birgit Czermin
    Adolf Butenandt Institut, University of Munich, Schillerstrasse 44, 80336 Munich, Germany
    Cell 111:185-96. 2002
  10. ncbi The histone H3 lysine-27 demethylase Jmjd3 links inflammation to inhibition of polycomb-mediated gene silencing
    Francesca De Santa
    Department of Experimental Oncology, European Institute of Oncology, Campus IFOM IEO, Via Adamello 16, 20139 Milan, Italy
    Cell 130:1083-94. 2007

Detail Information

Publications381 found, 100 shown here

  1. doi Lysine acetylation targets protein complexes and co-regulates major cellular functions
    Chunaram Choudhary
    Proteomics and Signal Transduction, Max Planck Institute for Biochemistry, Martinsried, Germany
    Science 325:834-40. 2009
    b>Lysine acetylation is a reversible posttranslational modification of proteins and plays a key role in regulating gene expression. Technological limitations have so far prevented a global analysis of lysine acetylation's cellular roles...
  2. pmc Molecular interplay of the noncoding RNA ANRIL and methylated histone H3 lysine 27 by polycomb CBX7 in transcriptional silencing of INK4a
    Kyoko L Yap
    Department of Structural and Chemical Biology, Mount Sinai School of Medicine, New York, NY 10029, USA
    Mol Cell 38:662-74. 2010
    ..tumor suppressor locus in normal and cancerous cell growth is controlled by methylation of histone H3 at lysine 27 (H3K27me) as directed by the Polycomb group proteins...
  3. doi Quantitative interaction proteomics and genome-wide profiling of epigenetic histone marks and their readers
    Michiel Vermeulen
    Department of Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, D 82152 Martinsried, Germany
    Cell 142:967-80. 2010
    Trimethyl-lysine (me3) modifications on histones are the most stable epigenetic marks and they control chromatin-mediated regulation of gene expression...
  4. ncbi Loss of acetylation at Lys16 and trimethylation at Lys20 of histone H4 is a common hallmark of human cancer
    Mario F Fraga
    Cancer Epigenetics Laboratory, Molecular Pathology Program, Spanish National Cancer Center, Melchor Fernandez Almagro 3, 28029 Madrid, Spain
    Nat Genet 37:391-400. 2005
    ..Our data suggest that the global loss of monoacetylation and trimethylation of histone H4 is a common hallmark of human tumor cells...
  5. ncbi Histone methyltransferase activity of a Drosophila Polycomb group repressor complex
    Jurg Muller
    EMBL, Gene Expression Programme, Meyerhofstr 1, 69117 Heidelberg, Germany
    Cell 111:197-208. 2002
    ..A recombinant complex reconstituted from these four subunits methylates lysine-27 of histone H3...
  6. ncbi Methylation of lysine 4 on histone H3: intricacy of writing and reading a single epigenetic mark
    Alexander J Ruthenburg
    Laboratory of Chromatin Biology, The Rockefeller University, New York, NY 10021, USA
    Mol Cell 25:15-30. 2007
    ..Here, we focus on one such element of variation: methylation of lysine 4 in histone H3 (H3K4)...
  7. ncbi The diverse functions of histone lysine methylation
    Cyrus Martin
    Howard Hughes Medical Institute, Department of Biochemistry and Biophysics, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, North Carolina 27599 7295, USA
    Nat Rev Mol Cell Biol 6:838-49. 2005
    ..One such modification, lysine methylation, has important functions in many biological processes that include heterochromatin formation, X-..
  8. ncbi Histone H4-K16 acetylation controls chromatin structure and protein interactions
    Michael Shogren-Knaak
    Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Science 311:844-7. 2006
    Acetylation of histone H4 on lysine 16 (H4-K16Ac) is a prevalent and reversible posttranslational chromatin modification in eukaryotes...
  9. ncbi Drosophila enhancer of Zeste/ESC complexes have a histone H3 methyltransferase activity that marks chromosomal Polycomb sites
    Birgit Czermin
    Adolf Butenandt Institut, University of Munich, Schillerstrasse 44, 80336 Munich, Germany
    Cell 111:185-96. 2002
    ..We show that this complex contains a methyltransferase activity that methylates lysine 9 and lysine 27 of histone H3, but the activity is lost when the E(Z) SET domain is mutated...
  10. ncbi The histone H3 lysine-27 demethylase Jmjd3 links inflammation to inhibition of polycomb-mediated gene silencing
    Francesca De Santa
    Department of Experimental Oncology, European Institute of Oncology, Campus IFOM IEO, Via Adamello 16, 20139 Milan, Italy
    Cell 130:1083-94. 2007
    ..group (PcG) proteins mediate gene silencing and repress transdifferentiation in a manner dependent on histone H3 lysine 27 trimethylation (H3K27me3)...
  11. pmc Lysine acetylation: codified crosstalk with other posttranslational modifications
    Xiang Jiao Yang
    Molecular Oncology Group, Department of Medicine, McGill University Health Centre, Montreal, QC H3A1A1, Canada
    Mol Cell 31:449-61. 2008
    b>Lysine acetylation has emerged as a major posttranslational modification for histones...
  12. pmc Distinct epigenomic landscapes of pluripotent and lineage-committed human cells
    R David Hawkins
    Ludwig Institute for Cancer Research, La Jolla, CA 92093, USA
    Cell Stem Cell 6:479-91. 2010
    ..Our results provide new insights into epigenetic mechanisms underlying properties of pluripotency and cell fate commitment...
  13. ncbi HATs and HDACs: from structure, function and regulation to novel strategies for therapy and prevention
    X J Yang
    Molecular Oncology Group, Department of Medicine, McGill University Health Center, Montreal, Quebec, Canada
    Oncogene 26:5310-8. 2007
    Acetylation of the epsilon-amino group of a lysine residue was first discovered with histones in 1968, but the responsible enzymes, histone acetyltransferases and deacetylases, were not identified until the mid-1990s...
  14. pmc Structural basis for the methylation state-specific recognition of histone H4-K20 by 53BP1 and Crb2 in DNA repair
    Maria Victoria Botuyan
    Department of Biochemistry and Molecular Biology, Mayo Clinic College of Medicine, Rochester, MN 55905, USA
    Cell 127:1361-73. 2006
    Histone lysine methylation has been linked to the recruitment of mammalian DNA repair factor 53BP1 and putative fission yeast homolog Crb2 to DNA double-strand breaks (DSBs), but how histone recognition is achieved has not been ..
  15. pmc EZH1 mediates methylation on histone H3 lysine 27 and complements EZH2 in maintaining stem cell identity and executing pluripotency
    Xiaohua Shen
    Department of Pediatric Oncology, Dana Farber Cancer Institute, Children s Hospital and Harvard Medical School, Boston, MA 02115, USA
    Mol Cell 32:491-502. 2008
    ..Ezh1 partially complements Ezh2 in executing pluripotency during ESC differentiation, suggesting that cell-fate transitions require epigenetic specificity...
  16. doi Gene silencing in cancer by histone H3 lysine 27 trimethylation independent of promoter DNA methylation
    Yutaka Kondo
    Department of Leukemia, University of Texas M D Anderson Cancer Center, 1515 Holcombe Boulevard, Houston, Texas 77030, USA
    Nat Genet 40:741-50. 2008
    ..silencing in cancer cells is mediated by at least two distinct histone modifications, polycomb-based histone H3 lysine 27 trimethylation (H3K27triM) and H3K9 dimethylation...
  17. pmc Sirt3-mediated deacetylation of evolutionarily conserved lysine 122 regulates MnSOD activity in response to stress
    Randa Tao
    Howard Hughes Medical Institute and Molecular Radiation Oncology, Radiation Oncology Branch, Center for Cancer Research, NCI, NIH, Bethesda, MD 20892, USA
    Mol Cell 40:893-904. 2010
    ..MnSOD contains a nutrient- and ionizing radiation (IR)-dependent reversible acetyl-lysine that is hyperacetylated in Sirt3⁻/⁻ livers at 3 months of age...
  18. pmc Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways
    Matthew J Rardin
    Buck Institute for Research on Aging, Novato, CA 94945, USA
    Proc Natl Acad Sci U S A 110:6601-6. 2013
    ..Here, we use a rigorous label-free quantitative MS approach (called MS1 Filtering) to analyze changes in lysine acetylation from mouse liver mitochondria in the absence of SIRT3...
  19. ncbi The transcriptional coactivators p300 and CBP are histone acetyltransferases
    V V Ogryzko
    Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892 2753, USA
    Cell 87:953-9. 1996
    ..p300/CBP acetylates all four core histones in nucleosomes. These observations suggest that p300/CBP acetylates nucleosomes in concert with PCAF...
  20. pmc SIRT6 is a histone H3 lysine 9 deacetylase that modulates telomeric chromatin
    Eriko Michishita
    Department of Medicine, Division of Endocrinology, Gerontology and Metabolism, School of Medicine, Stanford University, Stanford, California 94305, USA
    Nature 452:492-6. 2008
    ..Here we show that the human SIRT6 protein is an NAD+-dependent, histone H3 lysine 9 (H3K9) deacetylase that modulates telomeric chromatin...
  21. pmc NSD2 links dimethylation of histone H3 at lysine 36 to oncogenic programming
    Alex J Kuo
    Department of Biology, Stanford University, Stanford, CA 94305, USA
    Mol Cell 44:609-20. 2011
    The histone lysine methyltransferase NSD2 (MMSET/WHSC1) is implicated in diverse diseases and commonly overexpressed in multiple myeloma due to a recurrent t(4;14) chromosomal translocation...
  22. pmc SIRT6 regulates TNF-α secretion through hydrolysis of long-chain fatty acyl lysine
    Hong Jiang
    Department of Chemistry and Chemical Biology, Cornell University, Ithaca, New York 14853, USA
    Nature 496:110-3. 2013
    ..Here we show that human SIRT6 efficiently removes long-chain fatty acyl groups, such as myristoyl, from lysine residues...
  23. pmc PHF8 mediates histone H4 lysine 20 demethylation events involved in cell cycle progression
    Wen Liu
    Howard Hughes Medical Institute, School of Medicine, University of California at San Diego, 9500 Gilman Drive, La Jolla, California 92093, USA
    Nature 466:508-12. 2010
    ..modifications are linked to an ever-expanding range of biological functions, the demethylases for histone H4 lysine 20 and their potential regulatory roles remain unknown...
  24. pmc PTIP associates with MLL3- and MLL4-containing histone H3 lysine 4 methyltransferase complex
    Young Wook Cho
    Nuclear Receptor Biology Section, Clinical Endocrinology Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    J Biol Chem 282:20395-406. 2007
    ..PTIP complex carries robust HMT activity and specifically methylates lysine 4 (K4) on histone H3...
  25. pmc Histone lysine methylation dynamics: establishment, regulation, and biological impact
    Joshua C Black
    Massachusetts General Hospital Cancer Center and Department of Medicine, Harvard Medical School, 13 th Street, Charlestown, MA 02129, USA
    Mol Cell 48:491-507. 2012
    Histone lysine methylation has emerged as a critical player in the regulation of gene expression, cell cycle, genome stability, and nuclear architecture...
  26. pmc Identification of 67 histone marks and histone lysine crotonylation as a new type of histone modification
    Minjia Tan
    Ben May Department of Cancer Research, The University of Chicago, Chicago, IL 60637, USA
    Cell 146:1016-28. 2011
    ..We further investigated one of the marks, lysine crotonylation (Kcr), confirming that it represents an evolutionarily-conserved histone posttranslational ..
  27. pmc CBP/p300-mediated acetylation of histone H3 on lysine 56
    Chandrima Das
    Department of Biochemistry and Molecular Genetics, University of Colorado School of Medicine, PO Box 6511, Aurora Colorado 80045, USA
    Nature 459:113-7. 2009
    Acetylation within the globular core domain of histone H3 on lysine 56 (H3K56) has recently been shown to have a critical role in packaging DNA into chromatin following DNA replication and repair in budding yeast...
  28. ncbi Role of histone H3 lysine 27 methylation in Polycomb-group silencing
    Ru Cao
    Department of Biochemistry and Biophysics, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 7295, USA
    Science 298:1039-43. 2002
    ..We demonstrate that the complex specifically methylates nucleosomal histone H3 at lysine 27 (H3-K27)...
  29. pmc SETDB1: a novel KAP-1-associated histone H3, lysine 9-specific methyltransferase that contributes to HP1-mediated silencing of euchromatic genes by KRAB zinc-finger proteins
    David C Schultz
    The Wistar Institute, Philadelphia, Pennsylvania 19104, USA
    Genes Dev 16:919-32. 2002
    ..Recent genetic and biochemical studies link H3-lysine 9 (H3-K9) methylation to HP1-mediated heterochromatin formation and gene silencing...
  30. doi Lysine methylation: beyond histones
    Xi Zhang
    Department of Biochemistry and Molecular Biology, University of Texas MD Anderson Cancer Center, Houston, 77030, USA
    Acta Biochim Biophys Sin (Shanghai) 44:14-27. 2012
    ..have been extensively studied, only a few proteins other than histones have been reported that can be modified by lysine methylation...
  31. ncbi Structure of a beta-TrCP1-Skp1-beta-catenin complex: destruction motif binding and lysine specificity of the SCF(beta-TrCP1) ubiquitin ligase
    Geng Wu
    Cellular Biochemistry and Biophysics Program, Memorial Sloan Kettering Cancer Center, New York, NY 10021, USA
    Mol Cell 11:1445-56. 2003
    ..leads to the model of SCF catalyzing ubiquitination by increasing the effective concentration of the substrate lysine at the E2 active site...
  32. pmc Mutation of A677 in histone methyltransferase EZH2 in human B-cell lymphoma promotes hypertrimethylation of histone H3 on lysine 27 (H3K27)
    Michael T McCabe
    Cancer Epigenetics Discovery Performance Unit, Cancer Research, Oncology Research and Development, GlaxoSmithKline, Collegeville, PA 19426, USA
    Proc Natl Acad Sci U S A 109:2989-94. 2012
    Trimethylation of histone H3 on lysine 27 (H3K27me3) is a repressive posttranslational modification mediated by the histone methyltransferase EZH2...
  33. ncbi Histone demethylation mediated by the nuclear amine oxidase homolog LSD1
    Yujiang Shi
    Department of Pathology, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA 02115, USA
    Cell 119:941-53. 2004
    ..LSD1 specifically demethylates histone H3 lysine 4, which is linked to active transcription...
  34. ncbi Stat3 dimerization regulated by reversible acetylation of a single lysine residue
    Zheng Long Yuan
    Department of Surgery, Brown University Medical School Rhode Island Hospital, Providence, RI 02903, USA
    Science 307:269-73. 2005
    ..We show that in response to cytokine treatment, Stat3 is also acetylated on a single lysine residue, Lys685...
  35. pmc DNMT3L connects unmethylated lysine 4 of histone H3 to de novo methylation of DNA
    Steen K T Ooi
    Department of Genetics and Development, College of Physicians and Surgeons of Columbia University, New York, New York 10032, USA
    Nature 448:714-7. 2007
    ..interacts with the extreme amino terminus of histone H3; this interaction was strongly inhibited by methylation at lysine 4 of histone H3 but was insensitive to modifications at other positions...
  36. ncbi Structure and ligand of a histone acetyltransferase bromodomain
    C Dhalluin
    Structural Biology Program, Department of Physiology and Biophysics, Mount Sinai School of Medicine, New York, New York 10029 6574, USA
    Nature 399:491-6. 1999
    ..of structural and site-directed mutagenesis studies that bromodomains can interact specifically with acetylated lysine, making them the first known protein modules to do so...
  37. doi KAP1 controls endogenous retroviruses in embryonic stem cells
    Helen M Rowe
    School of Life Sciences, Ecole Polytechnique Federale de Lausanne, 1015 Lausanne, Switzerland
    Nature 463:237-40. 2010
    ..enriched at the 5' untranslated region (5'UTR) of IAP genomes, where KAP1 deletion leads to the loss of histone 3 lysine 9 trimethylation (H3K9me3), a hallmark of KAP1-mediated repression...
  38. ncbi Akt-mediated phosphorylation of EZH2 suppresses methylation of lysine 27 in histone H3
    Tai Lung Cha
    Department of Molecular and Cellular Oncology, The University of Texas M D Anderson Cancer Center, Houston, TX 77030, USA
    Science 310:306-10. 2005
    ..a methyltransferase that plays an important role in many biological processes through its ability to trimethylate lysine 27 in histone H3...
  39. pmc MMSET regulates histone H4K20 methylation and 53BP1 accumulation at DNA damage sites
    Huadong Pei
    Division of Oncology Research, Mayo Clinic, Rochester, Minnesota 55905, USA
    Nature 470:124-8. 2011
    ..1 (53BP1) is known to be an important mediator of the DNA damage response, with dimethylation of histone H4 lysine 20 (H4K20me2) critical to the recruitment of 53BP1 to double-strand breaks (DSBs)...
  40. ncbi Selective anchoring of TFIID to nucleosomes by trimethylation of histone H3 lysine 4
    Michiel Vermeulen
    Department of Proteomics and Signal Transduction, Max Planck Institute for Biochemistry, D 82152 Martinsried, Germany
    Cell 131:58-69. 2007
    Trimethylation of histone H3 at lysine 4 (H3K4me3) is regarded as a hallmark of active human promoters, but it remains unclear how this posttranslational modification links to transcriptional activation...
  41. ncbi Polycomb-mediated methylation on Lys27 of histone H3 pre-marks genes for de novo methylation in cancer
    Yeshayahu Schlesinger
    Department of Cellular Biochemistry and Human Genetics, The Hebrew University Hadassah Medical School, Jerusalem 91120, Israel
    Nat Genet 39:232-6. 2007
    ..These results suggest that tumor-specific targeting of de novo methylation is pre-programmed by an established epigenetic system that normally has a role in marking embryonic genes for repression...
  42. pmc Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns
    Alicia Lundby
    Novo Nordisk Foundation Center for Protein Research, Department for Proteomics, Faculty of Health Sciences, University of Copenhagen, Denmark
    Cell Rep 2:419-31. 2012
    b>Lysine acetylation is a major posttranslational modification involved in a broad array of physiological functions...
  43. doi Lysine succinylation is a frequently occurring modification in prokaryotes and eukaryotes and extensively overlaps with acetylation
    Brian T Weinert
    The NNF Center for Protein Research, Faculty of Health and Medical Sciences, University of Copenhagen, Blegdamsvej 3b, 2200 Copenhagen, Denmark
    Cell Rep 4:842-51. 2013
    ..These data provide a systems-wide view of succinylation and its dynamic regulation and show its extensive overlap with acetylation. ..
  44. doi Set2 methylation of histone H3 lysine 36 suppresses histone exchange on transcribed genes
    Swaminathan Venkatesh
    Stowers Institute for Medical Research, 1000 E 50th Street, Kansas City, Missouri 64110, USA
    Nature 489:452-5. 2012
    ..By suppressing spurious cryptic transcripts from initiating within ORFs, this pathway is essential to maintain the accuracy of transcription by RNA polymerase II...
  45. pmc Histone methyltransferase activity associated with a human multiprotein complex containing the Enhancer of Zeste protein
    Andrei Kuzmichev
    Howard Hughes Medical Institute, Division of Nucleic Acids Enzymology, Department of Biochemistry, University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, Piscataway, New Jersey 08854, USA
    Genes Dev 16:2893-905. 2002
    ..The presence of Su(z)12, a strong suppressor of position effect variegation, in PRC2 suggests that PRC2 may play a widespread role in heterochromatin-mediated silencing...
  46. ncbi Methylation of histone H4 lysine 20 controls recruitment of Crb2 to sites of DNA damage
    Steven L Sanders
    The Wellcome Trust Cancer Research UK Gurdon Institute and Department of Pathology, Tennis Court Road, Cambridge CB2 1QN, United Kingdom
    Cell 119:603-14. 2004
    Histone lysine methylation is a key regulator of gene expression and heterochromatin function, but little is known as to how this modification impinges on other chromatin activities...
  47. pmc A chromatin landmark and transcription initiation at most promoters in human cells
    Matthew G Guenther
    Whitehead Institute for Biomedical Research, Cambridge, MA 02142, USA
    Cell 130:77-88. 2007
    ..These observations extend to differentiated cells, suggesting that transcription initiation at most genes is a general phenomenon in human cells...
  48. pmc Lysine-specific demethylase 2B (KDM2B)-let-7-enhancer of zester homolog 2 (EZH2) pathway regulates cell cycle progression and senescence in primary cells
    Alexandros Tzatsos
    Cancer Center, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts 02114, USA
    J Biol Chem 286:33061-9. 2011
    ....
  49. ncbi Polymeric chains of SUMO-2 and SUMO-3 are conjugated to protein substrates by SAE1/SAE2 and Ubc9
    M H Tatham
    Institute of Biomolecular Sciences, University of St Andrews, North Haugh, St Andrews KY16 5ST, United Kingdom
    J Biol Chem 276:35368-74. 2001
    ..The ability to form polymeric chains is not shared by SUMO-1, and although all SUMO species use the same conjugation machinery, modification by SUMO-1 and SUMO-2/-3 may have distinct functional consequences...
  50. pmc Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae
    Peter Henriksen
    Department of Proteomics, The Novo Nordisk Foundation Center for Protein Research, Faculty of Health Sciences, University of Copenhagen, Blegdamsvej 3, DK 2200 Copenhagen, Denmark
    Mol Cell Proteomics 11:1510-22. 2012
    Post-translational modification of proteins by lysine acetylation plays important regulatory roles in living cells. The budding yeast Saccharomyces cerevisiae is a widely used unicellular eukaryotic model organism in biomedical research...
  51. pmc Large histone H3 lysine 9 dimethylated chromatin blocks distinguish differentiated from embryonic stem cells
    Bo Wen
    Center for Epigenetics, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    Nat Genet 41:246-50. 2009
    ..We have taken a genome-wide view of histone H3 lysine 9 dimethylation (H3K9Me2) and find that differentiated tissues show surprisingly large K9-modified regions (up to ..
  52. pmc Aberrant patterns of H3K4 and H3K27 histone lysine methylation occur across subgroups in medulloblastoma
    Adrian M Dubuc
    Division of Neurosurgery, Arthur and Sonia Labatt Brain Tumour Research Centre, The Hospital for Sick Children, Toronto, ON, Canada
    Acta Neuropathol 125:373-84. 2013
    ..As PRC2-mediated aberrant methylation of H3K27 has recently been targeted for therapy in other diseases, it represents an actionable target for a substantial percentage of medulloblastoma patients with aggressive forms of the disease...
  53. pmc Histone H4 lysine 16 acetylation regulates cellular lifespan
    Weiwei Dang
    Gene Expression and Regulation Program, The Wistar Institute Philadelphia, Pennsylvania 19104, USA
    Nature 459:802-7. 2009
    ..the first member of the family to be found, establishes and maintains chromatin silencing by removing histone H4 lysine 16 acetylation and bringing in other silencing proteins...
  54. pmc MOF and histone H4 acetylation at lysine 16 are critical for DNA damage response and double-strand break repair
    Girdhar G Sharma
    Washington University School of Medicine, St Louis, Missouri 63108, USA
    Mol Cell Biol 30:3582-95. 2010
    The human MOF gene encodes a protein that specifically acetylates histone H4 at lysine 16 (H4K16ac)...
  55. ncbi Role of histone H3 lysine 9 methylation in epigenetic control of heterochromatin assembly
    J Nakayama
    Cold Spring Harbor Laboratory, Post Office Box 100, Cold Spring Harbor, NY 11724, USA
    Science 292:110-3. 2001
    ..We provide in vivo evidence that lysine 9 of histone H3 (H3 Lys9) is preferentially methylated by the Clr4 protein at heterochromatin-associated regions ..
  56. pmc Recognition of trimethylated histone H3 lysine 4 facilitates the recruitment of transcription postinitiation factors and pre-mRNA splicing
    Robert J Sims
    Department of Biochemistry, New York University School of Medicine, New York, NY 10016, USA
    Mol Cell 28:665-76. 2007
    Trimethylation of histone H3 on lysine 4 (H3K4me3) localizes near the 5' region of genes and is tightly associated with active loci...
  57. pmc The Dnmt3a PWWP domain reads histone 3 lysine 36 trimethylation and guides DNA methylation
    Arunkumar Dhayalan
    Biochemistry Laboratory, School of Engineering and Science, Jacobs University Bremen, Bremen, Germany
    J Biol Chem 285:26114-20. 2010
    ..domain with modified histone tails using peptide arrays and show that it specifically recognizes the histone 3 lysine 36 trimethylation mark...
  58. pmc Chromatin signature of embryonic pluripotency is established during genome activation
    Nadine L Vastenhouw
    Department of Molecular and Cellular Biology, Harvard University, 16 Divinity Avenue, Cambridge, Massachusetts 02138, USA
    Nature 464:922-6. 2010
    ..that accompany pluripotency and genome activation, we mapped the genomic locations of histone H3 molecules bearing lysine trimethylation modifications before and after the maternal-zygotic transition in zebrafish...
  59. pmc A human protein complex homologous to the Drosophila MSL complex is responsible for the majority of histone H4 acetylation at lysine 16
    Edwin R Smith
    Department of Biology, Emory University, 1510 Clifton Road NE, Atlanta, GA 30322, USA
    Mol Cell Biol 25:9175-88. 2005
    ..This complex shows strong specificity for histone H4 lysine 16 in chromatin in vitro, and RNA interference-mediated knockdown experiments reveal that it is responsible for ..
  60. ncbi N(epsilon)-(carboxymethyl)lysine adducts of proteins are ligands for receptor for advanced glycation end products that activate cell signaling pathways and modulate gene expression
    T Kislinger
    College of Physicians and Surgeons, Columbia University, New York, New York 10032, USA
    J Biol Chem 274:31740-9. 1999
    ..specific AGEs within the tissues in disorders such as diabetes and renal failure, N(epsilon)-(carboxymethyl)lysine (CML) adducts, are ligands of RAGE...
  61. doi Biochemical mechanisms of gene regulation by polycomb group protein complexes
    Jurg Muller
    EMBL, Gene Expression Programme, Heidelberg, Germany
    Curr Opin Genet Dev 19:150-8. 2009
    ..Rather, PcG mediated repression also constitutes a mechanism for dynamic control of gene transcription...
  62. ncbi Activation of IKK by TNFalpha requires site-specific ubiquitination of RIP1 and polyubiquitin binding by NEMO
    Chee Kwee Ea
    Howard Hughes Medical Institute, Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA
    Mol Cell 22:245-57. 2006
    ..These results reveal the biochemical mechanism underlying the essential signaling function of NEMO and provide direct evidence that signal-induced site-specific ubiquitination of RIP1 is required for IKK activation...
  63. pmc Plasmodium falciparum heterochromatin protein 1 binds to tri-methylated histone 3 lysine 9 and is linked to mutually exclusive expression of var genes
    Karla Pérez-Toledo
    Departamento de Biomedicina Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional IPN, Apartado Postal 14 740, 07360, Mexico D F, Mexico
    Nucleic Acids Res 37:2596-606. 2009
    ..Recombinant CD binds to di- and tri-methylated lysine 9 from histone H3, but not to unmodified or methylated histone H3 in lysine 4...
  64. pmc Histone H3 lysine 4 trimethylation marks meiotic recombination initiation sites
    Valerie Borde
    Institut Curie Centre de Recherche, UMR7147 CNRS, Universite Pierre et Marie Curie, Paris, France
    EMBO J 28:99-111. 2009
    ..In Saccharomyces cerevisiae, we examined the genome-wide localization of histone H3 lysine 4 trimethylation (H3K4me3) along meiosis and its relationship to gene expression and position of the programmed ..
  65. pmc Acetylation of metabolic enzymes coordinates carbon source utilization and metabolic flux
    Qijun Wang
    State Key Laboratory of Genetic Engineering, Department of Microbiology, School of Life Sciences and Institute of Biomedical Sciences, Fudan University, Shanghai 200032, China
    Science 327:1004-7. 2010
    b>Lysine acetylation regulates many eukaryotic cellular processes, but its function in prokaryotes is largely unknown...
  66. ncbi Regulation of heterochromatic silencing and histone H3 lysine-9 methylation by RNAi
    Thomas A Volpe
    Cold Spring Harbor Laboratory, Watson School of Biological Sciences, Cold Spring Harbor, NY 11724, USA
    Science 297:1833-7. 2002
    ..is accompanied by transcriptional de-repression of transgenes integrated at the centromere, loss of histone H3 lysine-9 methylation, and impairment of centromere function...
  67. ncbi A histone H3 lysine 27 demethylase regulates animal posterior development
    Fei Lan
    Department of Pathology, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, Massachusetts 02115, USA
    Nature 449:689-94. 2007
    ..Taken together, these findings identify a small family of H3K27 demethylases with important, evolutionarily conserved roles in H3K27 methylation regulation and in animal anterior-posterior development...
  68. ncbi Substrate and functional diversity of lysine acetylation revealed by a proteomics survey
    Sung Chan Kim
    Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA
    Mol Cell 23:607-18. 2006
    Acetylation of proteins on lysine residues is a dynamic posttranslational modification that is known to play a key role in regulating transcription and other DNA-dependent nuclear processes...
  69. ncbi WDR5 associates with histone H3 methylated at K4 and is essential for H3 K4 methylation and vertebrate development
    Joanna Wysocka
    Laboratory of Chromatin Biology, The Rockefeller University, New York, New York 10021, USA
    Cell 121:859-72. 2005
    Histone H3 lysine 4 (K4) methylation has been linked to the transcriptional activation in a variety of eukaryotic species...
  70. doi The histone H4 Lys 20 methyltransferase PR-Set7 regulates replication origins in mammalian cells
    Mathieu Tardat
    Institut de Génétique Moléculaire de Montpellier IGMM, UMR 5535 CNRS, Montpellier, France
    Nat Cell Biol 12:1086-93. 2010
    ..These results elucidate a critical role for PR-Set7 and H4K20me1 in the chromatin events that regulate replication origins...
  71. ncbi Selective recognition of methylated lysine 9 on histone H3 by the HP1 chromo domain
    A J Bannister
    Wellcome CRC Institute and Department of Pathology, University of Cambridge, UK
    Nature 410:120-4. 2001
    ..Both Clr4 and SUV39H1 methylate specifically lysine 9 of histone H3 (ref. 6)...
  72. pmc The genomic landscape of histone modifications in human T cells
    Tae Young Roh
    Laboratory of Molecular Immunology, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 103:15782-7. 2006
    ..Therefore, these regions have a distinctive chromatin modification pattern and thus may represent a distinct class of chromatin domains...
  73. pmc The tRNA synthetase paralog PoxA modifies elongation factor-P with (R)-β-lysine
    Hervé Roy
    Department of Microbiology, Center for RNA Biology, Ohio State University, Columbus, Ohio, USA
    Nat Chem Biol 7:667-9. 2011
    The lysyl-tRNA synthetase paralog PoxA modifies elongation factor P (EF-P) with α-lysine at low efficiency...
  74. ncbi Methylation of histone H3R2 by PRMT6 and H3K4 by an MLL complex are mutually exclusive
    Ernesto Guccione
    Department of Experimental Oncology, European Institute of Oncology IEO, IFOM IEO Campus, Milan 20139, Italy
    Nature 449:933-7. 2007
    ..di-methylation of histone H3 arginine 2 (H3R2me2a) counter-correlates with di- and tri- methylation of H3 lysine 4 (H3K4me2, H3K4me3) on human promoters...
  75. pmc PARP1 ADP-ribosylates lysine residues of the core histone tails
    Simon Messner
    Institute of Veterinary Biochemistry and Molecular Biology, Life Science Zurich Graduate School, University of Zurich, Winterthurerstrasse 190, 8057 Zurich, Switzerland
    Nucleic Acids Res 38:6350-62. 2010
    ..Taken together, our computational and experimental results provide strong evidence that PARP1 modifies important regulatory lysines of the core histone tails...
  76. ncbi Lysine 63-linked ubiquitination promotes the formation and autophagic clearance of protein inclusions associated with neurodegenerative diseases
    Jeanne M M Tan
    Neurodegeneration Research Lab, National Neuroscience Institute, Singapore, Singapore
    Hum Mol Genet 17:431-9. 2008
    ..Here, we report that lysine 48 (K48)- and lysine 63 (K63)-linked polyubiquitination, as well as monoubiquitin modification contribute to the ..
  77. pmc Amplification of siRNA in Caenorhabditis elegans generates a transgenerational sequence-targeted histone H3 lysine 9 methylation footprint
    Sam Guoping Gu
    Department of Pathology, Stanford University School of Medicine, Stanford, California, USA
    Nat Genet 44:157-64. 2012
    ..profiling, we found that a diverse set of genes can be induced to acquire locus-specific enrichment of histone H3 lysine 9 trimethylation (H3K9me3), with modification footprints extending several kilobases from the site of dsRNA ..
  78. ncbi Methylation of H3-lysine 79 is mediated by a new family of HMTases without a SET domain
    Qin Feng
    Department of Biochemistry and Biophysics, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, 27599, USA
    Curr Biol 12:1052-8. 2002
    ..Histone methylation occurs on arginine and lysine residues and is catalyzed by two families of proteins, the protein arginine methyltransferase family and the SET-..
  79. ncbi Methylated lysine 79 of histone H3 targets 53BP1 to DNA double-strand breaks
    Yentram Huyen
    Wistar Institute, Philadelphia, Pennsylvania 19104 4268, USA
    Nature 432:406-11. 2004
    ..Because methylation of histone H3 Lys 79 was unaltered in response to DNA damage, we propose that 53BP1 senses DSBs indirectly through changes in higher-order chromatin structure that expose the 53BP1 binding site...
  80. pmc A unique H3K4me2 profile marks tissue-specific gene regulation
    Aleksandra Pekowska
    Centre d immunologie de Marseille Luminy, Universite Aix Marseille, Marseille 13009, France
    Genome Res 20:1493-502. 2010
    ..we used high-throughput genomic data from CD4(+) T lymphocytes to provide a comprehensive analysis of histone H3 lysine 4 dimethylation (H3K4me2) enrichment in genomic regions surrounding transcriptional start sites (TSSs)...
  81. ncbi Acetylation of the C terminus of Ku70 by CBP and PCAF controls Bax-mediated apoptosis
    Haim Y Cohen
    Department of Pathology, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA 02115 USA
    Mol Cell 13:627-38. 2004
    ..We demonstrate that increased acetylation of Ku70 disrupts the Ku70-Bax interaction and coincides with cytoplasmic accumulation of CBP. These results shed light on the role of acetyltransferases as tumor suppressors...
  82. pmc Global analysis of lysine ubiquitination by ubiquitin remnant immunoaffinity profiling
    Guoqiang Xu
    Department of Pharmacology, Weill Medical College, Cornell University, New York, New York, USA
    Nat Biotechnol 28:868-73. 2010
    ..To address this issue, we generated a monoclonal antibody that enriches for peptides containing lysine residues modified by diglycine, an adduct left at sites of ubiquitination after trypsin digestion...
  83. pmc Mammalian Sir2 homolog SIRT3 regulates global mitochondrial lysine acetylation
    David B Lombard
    Howard Hughes Medical Institute, The Children s Hospital, Harvard Medical School, Boston, MA 02115, USA
    Mol Cell Biol 27:8807-14. 2007
    ..Overall, our results extend the recent finding of lysine acetylation of mitochondrial proteins and demonstrate that SIRT3 has evolved to control reversible lysine ..
  84. pmc Reversible lysine acetylation controls the activity of the mitochondrial enzyme acetyl-CoA synthetase 2
    Bjoern Schwer
    Gladstone Institute of Virology and Immunology, University of California, San Francisco, CA 94158, USA
    Proc Natl Acad Sci U S A 103:10224-9. 2006
    ..findings show that a mammalian sirtuin directly controls the activity of a metabolic enzyme by means of reversible lysine acetylation...
  85. pmc The polycomb group protein Suz12 is required for embryonic stem cell differentiation
    Diego Pasini
    Centre for Epigenetics and BRIC, University of Copenhagen, Ole Maaløes Vej 5, 2200 Copenhagen, Denmark
    Mol Cell Biol 27:3769-79. 2007
    ..PRC2 contains the PcG proteins EZH2, SUZ12, and EED and represses transcription through methylation of lysine (K) 27 of histone H3 (H3)...
  86. doi Proteome-wide mapping of the Drosophila acetylome demonstrates a high degree of conservation of lysine acetylation
    Brian T Weinert
    Department of Proteomics, The Novo Nordisk Foundation Center for Protein Research, Faculty of Health Sciences, University of Copenhagen, Blegdamsvej 3, 2200 Copenhagen, Denmark
    Sci Signal 4:ra48. 2011
    ..With high-resolution mass spectrometry, we identified 1981 lysine acetylation sites in the proteome of Drosophila melanogaster...
  87. pmc Role of the polycomb protein EED in the propagation of repressive histone marks
    Raphael Margueron
    Howard Hughes Medical Institute and Department of Biochemistry, New York University Medical School, 522 First Avenue, New York, New York 10016, USA
    Nature 461:762-7. 2009
    ..The gene-silencing activity of the Polycomb repressive complex 2 (PRC2) depends on its ability to trimethylate lysine 27 of histone H3 (H3K27) by the catalytic SET domain of the EZH2 subunit, and at least two other subunits of the ..
  88. ncbi Post-activation turn-off of NF-kappa B-dependent transcription is regulated by acetylation of p65
    Rosemary Kiernan
    Laboratoire de Virologie Moleculaire, Institut de Genetique Humaine, Montpellier 34296, France
    J Biol Chem 278:2758-66. 2003
    ..We propose that acetylation of p65 plays a key role in IkappaBetaalpha-mediated attenuation of NF-kappaBeta transcriptional activity which is an important process that restores the latent state in post-induced cells...
  89. ncbi Establishment of histone h3 methylation on the inactive X chromosome requires transient recruitment of Eed-Enx1 polycomb group complexes
    Jose Silva
    X Inactivation Group, MRC Clinical Sciences Centre, ICSM, Hammersmith Hospital, Du Cane Road, London W12 0NN, United Kingdom
    Dev Cell 4:481-95. 2003
    ..Functional analysis demonstrates that Eed-Enx1 is required to establish methylation of histone H3 at lysine 9 and/or lysine 27 on Xi and that this, in turn, is required to stabilize the Xi chromatin structure.
  90. pmc The CHD3 remodeler PICKLE associates with genes enriched for trimethylation of histone H3 lysine 27
    Heng Zhang
    Department of Biochemistry, Purdue University, West Lafayette, Indiana 47907, USA
    Plant Physiol 159:418-32. 2012
    ..PKL promotes the epigenetic mark trimethylation of histone H3 lysine 27 (H3K27me3) that facilitates repression of tissue-specific genes in plants...
  91. pmc Position-specific analysis and prediction for protein lysine acetylation based on multiple features
    Sheng Bao Suo
    Department of Chemistry, Nanchang University, Nanchang, China
    PLoS ONE 7:e49108. 2012
    Protein lysine acetylation is a type of reversible post-translational modification that plays a vital role in many cellular processes, such as transcriptional regulation, apoptosis and cytokine signaling...
  92. doi Dense chromatin activates Polycomb repressive complex 2 to regulate H3 lysine 27 methylation
    Wen Yuan
    College of Biological Sciences, China Agricultural University, Beijing 100094, China
    Science 337:971-5. 2012
    Polycomb repressive complex 2 (PRC2)-mediated histone H3 lysine 27 (H3K27) methylation is vital for Polycomb gene silencing, a classic epigenetic phenomenon that maintains transcriptional silencing throughout cell divisions...
  93. pmc CpG islands recruit a histone H3 lysine 36 demethylase
    Neil P Blackledge
    Department of Biochemistry, University of Oxford, Oxford, UK
    Mol Cell 38:179-90. 2010
    ..Here we show that CpG islands directly recruit the H3K36-specific lysine demethylase enzyme KDM2A...
  94. doi Lysine methylation of VCP by a member of a novel human protein methyltransferase family
    Stefan Kernstock
    Department of Molecular Biosciences, Faculty of Mathematics and Natural Sciences, University of Oslo, Oslo 0316 Norway
    Nat Commun 3:1038. 2012
    ..a variety of cell lines and tissues, and that the previously uncharacterized protein METTL21D (denoted here as VCP lysine methyltransferase, VCP-KMT) is the responsible enzyme...
  95. pmc Sirt5 is a NAD-dependent protein lysine demalonylase and desuccinylase
    Jintang Du
    Department of Chemistry and Chemical Biology, Cornell University, Ithaca, NY 14853, USA
    Science 334:806-9. 2011
    ..We found that Sirt5 is an efficient protein lysine desuccinylase and demalonylase in vitro...
  96. pmc Arginine methylation at histone H3R2 controls deposition of H3K4 trimethylation
    Antonis Kirmizis
    Gurdon Institute and Department of Pathology, Tennis Court Road, Cambridge CB2 1QN, UK
    Nature 449:928-32. 2007
    ..Methylation at lysine 4 on histone H3 (H3K4) is found at the 5' end of active genes and contributes to transcriptional activation by ..
  97. pmc Lysine acetylation is a highly abundant and evolutionarily conserved modification in Escherichia coli
    Junmei Zhang
    Department of Biochemistry, Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
    Mol Cell Proteomics 8:215-25. 2009
    b>Lysine acetylation and its regulatory enzymes are known to have pivotal roles in mammalian cellular physiology...
  98. pmc Dynamic acetylation of all lysine-4 trimethylated histone H3 is evolutionarily conserved and mediated by p300/CBP
    Nicholas T Crump
    Nuclear Signalling Laboratory, Department of Biochemistry, Oxford University, Oxford OX1 3QU, United Kingdom
    Proc Natl Acad Sci U S A 108:7814-9. 2011
    ..Furthermore, dynamic acetylation targeted to H3K4me3 is mediated by the same lysine acetyltransferase, p300/cAMP response element binding (CREB)-binding protein (CBP), in both mouse and fly cells...
  99. pmc Genome-wide association of histone H3 lysine nine methylation with CHG DNA methylation in Arabidopsis thaliana
    Yana V Bernatavichute
    Molecular Biology Institute, University of California Los Angeles, Los Angeles, California, United States of America
    PLoS ONE 3:e3156. 2008
    Methylation of histone H3 lysine 9 (H3K9) is a hallmark of transcriptional silencing in many organisms...
  100. pmc Cross-talk between phosphorylation and lysine acetylation in a genome-reduced bacterium
    Vera van Noort
    Structural and Computational Biology Unit, European Molecular Biology Laboratory, EMBL, Heidelberg, Germany
    Mol Syst Biol 8:571. 2012
    ..only the protein-specific effect on the phosphorylation network, but also a modulation of proteome abundance and lysine acetylation patterns, mostly in the absence of transcriptional changes...
  101. pmc The first identification of lysine malonylation substrates and its regulatory enzyme
    Chao Peng
    Ben May Department for Cancer Research, The University of Chicago, Chicago, IL 60637, USA
    Mol Cell Proteomics 10:M111.012658. 2011
    Protein post-translational modifications (PTMs) at the lysine residue, such as lysine methylation, acetylation, and ubiquitination, are diverse, abundant, and dynamic. They play a key role in the regulation of diverse cellular physiology...

Research Grants75

  1. Lysine decarboxylase in periodontitis patients
    Martin Levine; Fiscal Year: 2004
    ..We posit that lysine decarboxylase (LDC), a bacterial enzyme in the sulcus, depletes the transudate of lysine, starving the DAT cells...
  2. Ligand Directed Transdermal siRNA Delivery to Improve Wound Healing
    David M Evans; Fiscal Year: 2012
    ..siRNA cocktail targeting these genes can accelerate wound repair in acute wounds when administered in Histidine Lysine Polymer (HKP) - a branched cationic peptide...
  3. Angiotensin Adrenergic Interactions in the Kidney
    Roland C Blantz; Fiscal Year: 2013
    ..T2) did not normalize QO2/TNa nor did the increase in GFR that accompanies AII blockade by administration of lysine (A/I + T3) Several molecules and enzymes were overexpressed in the untreated A/I kidney including NADPH oxidase, ..
  4. Discovery of Small Molecule MBT Domain Antagonists
    STEPHEN VERNON FRYE; Fiscal Year: 2010
    ..The most important modifications of histones and DNA include: histone lysine and arginine methylation;lysine acetylation;DNA cytosine methylation;and histone sumoylation, ubiquitination, ADP-..
  5. Lung Lysyl Oxidase Regulation by Metal Ion Homeostasis
    Wande Li; Fiscal Year: 2013
    PROJECT ABSTRACT. Lysyl oxidase (LO), a copper-(Cu) dependent enzyme, oxidizes peptidyl lysine residues in substrates, e.g...
  6. REGULATION AND FUNCTION OF STE20-RELATED PROTEIN KINASES
    Melanie H Cobb; Fiscal Year: 2012
    DESCRIPTION (provided by applicant): WNK (With No lysine (K)) protein kinases are Ste20 relatives that we discovered through studying control of mitogen-activated protein kinases (MAPKs)...
  7. THE ROLE OF ACETYLATION IN MITOCHONDRIAL FUNCTION
    Gary Cecchini; Fiscal Year: 2013
    ..Reversible acetylation of lysine residues is one such modification that is receiving increasing attention...
  8. Remodeling Neuronal Chromatin in Mouse Models for Depression
    Schahram Akbarian; Fiscal Year: 2013
    ..mice with a sustained increase in neuronal expression of Setdb1, encoding a developmentally regulated histone H3-lysine 9 specific histone methyltransferase associated with Sin3a-deacetylase, KAP-1 and NuRD transcriptional repressor ..
  9. Fatty acid related regulation of enteric infectious disease
    FREDRICK JON KULL; Fiscal Year: 2012
    ..Two positively charged lysine side chains, one from each domain, play a critical role in fatty acid binding and, thereby, in the mechanism of ..
  10. Targeting iNOS to inhibit myeloid-derived suppressor cells (MDSC) in melanoma
    ANDREW GREGORY SIKORA; Fiscal Year: 2013
    ..Treatment with the small molecule iNOS inhibitor N6(1-iminoethyl)-L-lysine-dihydrochloride (L-NIL) decreases MDSC infiltration in tumor and spleen, reverses the tumor-mediated decrease in ..
  11. Regulation of LPS-mediated HMGB1 Release by Poly (ADP-ribose) Polymerase-1
    RAJESH K ANEJA; Fiscal Year: 2013
    ..The newly synthesized HMGB1 undergoes extensive post-translational modifications, e.g., acetylation of lysine residues that promote active transport of HMGB1 from the nucleus to the endosomal compartment and prevent its re-..
  12. Genetic &Functional Analyses of Hypertension Susceptibility Genes
    YEN PEI CHRISTY CHANG; Fiscal Year: 2013
    ..Mouse models indicated that members of this network, such as with no lysine kinases (WNKs) and STK39-encoded Ste20-related proline-alanine-rich kinase (SPAK), might have unknown isoforms ..
  13. Validation of a Novel NF-kB Inhibitor in Inflammatory Bowel Disease
    Anthony Blikslager; Fiscal Year: 2013
    ..TLX1423 is a peptide comprised of a 8 lysine (8K) protein transduction domain (PTD) with an I?B kinase (IKK) inhibitory sequence, "NF??B essential modulator" (..
  14. Evaluating the role of histone H3K79 methylation states in DNA damage repair in S
    Jeffrey S Thompson; Fiscal Year: 2010
    ..We and others have previously found that a specific histone modification, methylation of lysine-79 in histone H3 (H3K79), is important for the response to DNA damage caused by UV radiation...
  15. CONTROL OF EUCARYOTIC FUNCTION BY METHYLATION
    Steven G Clarke; Fiscal Year: 2013
    ..controlled by methylation reactions, focusing on the enzymes that catalyze the modification of protein arginine, lysine, and isoaspartyl residues...
  16. NAD Metabolism and Signaling
    Frances E Lund; Fiscal Year: 2011
    ..However, with the more recent discoveries showing that NAD is consumed by enzymes that deacetylate lysine and generate putative signaling metabolites (sirtuins), that post-translationally modify protein targets by ADP-..
  17. HTS development for targeted anti-fungal small molecules
    Paul D Kaufman; Fiscal Year: 2010
    ..A recently discovered fungal histone acetyltransferase (HAT) enzyme, termed Rtt109, acetylates histone H3 lysine 56, and is important for genome stability and resistance to genotoxic agents...
  18. Histone Methylation: a role in excessive ethanol intake and self-administration
    Jennifer A Rinker; Fiscal Year: 2012
    ..histone methyltransferases, G9a and G9a-like protein (GLP), and consequently reducing methylation of histone H3 on lysine 9 (H3K9) in the nucleus accumbens (NAc)...
  19. Varicella zoster virus: molecular controls of cell fusion-dependent pathogenesis
    Ann Arvin; Fiscal Year: 2013
    ..regulates intracellular signaling events in cell fusion via post-translational modifications of tyrosine and/r lysine residues by cellular or viral proteins...
  20. Deacetylation of mitochondrial proteins protect neurons from ischemic injury
    Conrad Alano; Fiscal Year: 2013
    ..Therefore, regulation of ROS production is a key target for ischemic stroke therapy. Post-translational lysine acetylation has recently emerged as an important regulator of gene expression and enzyme activity...
  21. The Role of Carboxypeptidase B and its Substrates in Rheumatoid Arthritis
    William H Robinson; Fiscal Year: 2013
    ..Carboxypeptidase B (CPB) is well established to play an anti-fibrinolytic role by removing C-terminal lysine residues from fibrin, thereby preventing its cleavage by plasmin...
  22. mTORC1-dependent regulation of the CycC/CDK8 complex
    Jeffrey E Pessin; Fiscal Year: 2013
    ..the CDK8 and CycC proteins;mTORC1-dependent regulation CDK8/CycC complex assembly state;and 3) ubiquitination of lysine residues in CDK8 and CycC...
  23. Post-translational regulation of MLL in leukemogenesis
    ANDREW GEORGE MUNTEAN; Fiscal Year: 2013
    ..MLL is a histone H3 lysine 4 methyltransferase transcription factor that regulates the expression of HOX genes, which are required for MLL ..
  24. Post-translational regulation of MLL in leukemogenesis
    ANDREW GEORGE MUNTEAN; Fiscal Year: 2012
    ..MLL is a histone H3 lysine 4 methyltransferase transcription factor that regulates the expression of HOX genes, which are required for MLL ..
  25. Regulation of the VDJ recombinase during genotoxic stress
    Karla K Rodgers; Fiscal Year: 2013
    ..the V(D)J recombinase from the genome by disrupting interactions between RAG2 and Histone H3 trimethylated on Lysine 4 (H3K4me3)...
  26. TRANSCRIPTIONAL REPRESSION BY POLYCOMB GROUP PRODUCTS
    Jeffrey A Simon; Fiscal Year: 2012
    ..PRC2 has four core subunits and an enzyme activity that methylates histone H3 on lysine-27. One Aim is to determine how the noncatalytic subunits make key inputs to PRC2 function in vitro and in vivo...
  27. Is symmetrical dimethylation of Arginine 2 of Histone H3 used as an epigenetic si
    Marjorie A Oettinger; Fiscal Year: 2010
    ..that RAG2 contains a plant homeodomain (PHD) finger that specifically recognizes histone H3 trimethylated on lysine 4, and exhibits an even stronger preference for binding H3 that is concurrently trimethylated on lysine 4 and ..
  28. Epigenetic mechanisms of TDP43-mediated neurodegeneration
    Amit Berson; Fiscal Year: 2013
    ..we found that several factors, all of which impact a single modification (trimethylation of histone 3 at lysine 4 (H3K4me3)), robustly modulate toxicity of TDP-43 in Drosophila, but not that of other neurodegeneration-related ..
  29. Transcriptional Regulation by a Deacetylase, CobB, in a Periodontopathogen
    DAVID J KOLODRUBETZ; Fiscal Year: 2013
    ..and archaeal transcription and that transcription factors can be acetylated in bacteria, we postulated that lysine acetylation of transcriptional regulatory proteins could play an important role in shaping the RNA expression ..
  30. Regulation of ENaC Transcription
    Bruce C Kone; Fiscal Year: 2013
    ..current project period, we discovered and characterized novel nuclear represor complexes containing the histone lysine-79 methyltransferase Dot1a together with either AF9 or Sirt1...
  31. Novel High Affinity and Long-Acting Bovine FSH Analogs for Veterinary Superovulat
    BRUCE DALE WEINTRAUB; Fiscal Year: 2012
    ..Develop novel high affinity recombinant bFSH superagonist analogs by minimal 4-5 arginine and/or lysine site-directed mutagenesis to produce a more potent and efficacious molecule with possible delayed absorption to ..
  32. Transcriptional regulation of enteroendocrine cell differentiation by NeuroD
    Andrew B Leiter; Fiscal Year: 2013
    ..preliminary results indicate interactions with other DNA binding proteins, CtBP, and the histone modifying enzyme, lysine specific demethylase 1 (LSD1) are involved...
  33. Epigenetic programming of brain development by choline nutrition
    Jan Krzysztof Blusztajn; Fiscal Year: 2011
    ..and 3) chromatin immunoprecipitation (ChIP) with antibodies against different methylated epitopes on specific lysine residues of histone 3 (e.g. H3K9me2;H3K4me1;H3K4me3) followed microarray analysis (ChIP-chip)...
  34. METABOLIC CONTROL OF SUGAR TRANSPORT
    Anthony Carruthers; Fiscal Year: 2012
    ..These changes involve specific loop 6 and C-terminal lysine residues and convert the cage to one which now prefers ?-D-glucose 20-fold over ?-D- glucose...
  35. Role of H-Ras in Retinal Cell Death in Diabetes
    Renu A Kowluru; Fiscal Year: 2013
    ..diabetes, the recruitment of p65 subunit of NF-kB is increased at retinal MMP-9 promoter and that of dimethylated lysine 9 of histone 3 (H3K9me2) is decreased, lysine-specific demethylase (LSD1) is activated and histone deacetylase (..
  36. PTIP as an epigenetic regulator in male meiosis
    Guo Zhang Zhu; Fiscal Year: 2013
    ..PTIP has been lately identified as part of a histone H3 lysine 4 (H3K4) methyltransferase complex and is essential for embryonic development...
  37. The Effects of Ethanol on Microtubules during Mouse Pre-implantation Development
    Yixian Zheng; Fiscal Year: 2012
    ..Acetylation of 1-tubulin at its lysine-40 residue has been shown to affect microtubule assembly dynamics and cell motility...
  38. PCAF/GCN5 ACETYLATION ON P53 TRANSACTIVATION
    Shelley L Berger; Fiscal Year: 2013
    ..We demonstrated that methylation and demethylation at lysine 370 (K370) are involved in regulating p53 in response to DNA damage...
  39. Regulation of mitochondrial metabolism by lysine acetylation
    Eric S Goetzman; Fiscal Year: 2013
    ..The key fatty acid oxidation enzyme long-chain acyl-CoA dehydrogenase (LCAD) has eight lysine acetylation sites and is demonstrated to be a target of the mitochondrial NAD-dependent deacetylase sirtuin-3 (..
  40. Enzymology of Post-translational Modifications
    Carol A Fierke; Fiscal Year: 2013
    ..that catalyze protein N-acetylation, and histone deacetylases (HDACs), that catalyze hydrolysis of 5-N-acetyl lysine. Acetylation affects multiple cellular processes, including gene expression and cell differentiation...
  41. Epidemiology of Retinopathy and other Complications in Long Term Type 1 Diabetes
    Michael Y Tsai; Fiscal Year: 2013
    ..g., AGEs [carboxymethyl lysine, pentosidine], soluble RAGE, oxidized low density lipoprotein cholesterol, creatinine) according to standardized ..
  42. Epigenome Mapping in Cortical Interneurons
    Schahram Akbarian; Fiscal Year: 2013
    ..Focus will be on tri- and mono-methyl-histone H3-lysine 4 (H3K4me3, H3K4me1) which are enriched at transcription start sites (H3K4me3) and enhancer sequences including ..
  43. Molecular mechanism of apolipoprotein binding to lipopolysaccharides
    PAUL MICHIEL WEERS; Fiscal Year: 2013
    ..iii) Since charge plays an important role in the LPS binding interaction with human apoA-I, key lysine residues in apoA-I necessary for LPS binding will be identified...
  44. Mdig gene and histone demethylation in lung cancer
    Fei Chen; Fiscal Year: 2013
    ..Mdig promotes cell growth and transformation by functioning as a histone demethylase that antagonizes tri-methyl lysine 9 on histone H3...
  45. Targeting Sirtuin-1 to enhance Foxp3+ regulatory T-cell function
    Ulf Beier; Fiscal Year: 2013
    ..We have shown that acetylation of lysine residues within the Foxp3 protein can enhance Treg function (2)...
  46. Histone Deacetylase Inhibition: A Novel Approach to Cardioprotection
    Ting C Zhao; Fiscal Year: 2012
    ..We have identified that p38 is acetylated at lysine 53 and lysine 121 sites...
  47. Sodium Chloride Cotransporter Regulation by WNK Kinase
    Hui Cai; Fiscal Year: 2010
    WNKs (with no lysine (K)) are a newly described novel subfamily of serine/threonine kinases implicated in controlling the ionic permeability of epithelia...
  48. Targeted epigenetic therapy of triple-negative breast cancer
    Samuel Waxman; Fiscal Year: 2013
    ..that regulates transcription by recruiting HDAC, BP2/JARID1A, a demethylase specific for di- and trimethylated lysine 4 of histone H3, critical for de novo DNA methylations and other chromatin modifiers...
  49. Inducible Dysplastic Nephropathy in B2-Deficient Mice
    Samir S El-Dahr; Fiscal Year: 2013
    ..in BdkrB2-/- kidneys is accompanied by histone deacetylase-1 recruitment, promoter histone H3 deacetylation and lysine 4 (H3K4) demethylation and H3K9/27 methylation, followed by hypermethylation of CpG islands...
  50. Genetic and Molecular Studies of Drosophila Chromatin Remodeling Factors
    John W Tamkun; Fiscal Year: 2013
    ..group proteins play important roles in the regulation of cellular pluripotency and differentiation by methylating lysine 27 of histone H3 over broad chromatin domains...
  51. Regulation of non-histone protein functions by histone deacetylases
    Edward Seto; Fiscal Year: 2010
    ..are enzymes that regulate the functions of histone proteins by catalyzing the removal of acetyl groups from lysine residues...
  52. Advanced Glycation End-Products and Risk of Pancreatic Cancer
    Li Jiao; Fiscal Year: 2013
    ..N[unreadable]-(carboxymethyl)-lysine (CML)-AGE is one of the best characterized AGEs...
  53. Mechanisms of Hormonal Regulation of the Sodium Chloride Cotransporter
    Robert S Hoover; Fiscal Year: 2013
    ..With-No-Lysine Kinase 1 and 4 (WNK1 and WNK4), Ste20-related proline alanine-rich kinase (SPAK) and Oxidative Stress Response-1 (..
  54. Non-coding RNAs in the epigenetics of human centromere formation
    PETER WARBURTON; Fiscal Year: 2009
    ..Protein A (CENP-A), a centromere-specific histone H3 variant, interspersed with Histone H3 dimethylated at lysine 4 (H3K4diMe)...
  55. Genetic and Epigenetic Regulation of Hematopoiesis.
    Shireen Saleque; Fiscal Year: 2013
    ..In light of our recent findings linking Gfi-1 and Gfi-1b to chromatin modifiers LSD1 (lysine specific demethylase1), CoREST/Rcor1 (REST co-repressor), other Rcor homologs and the histone deacetylases HDAC1-2,..
  56. Functional role of RAG2 H3K4me3 interaction in V(D)J recombination
    Marjorie A Oettinger; Fiscal Year: 2013
    ..that RAG2 contains a plant homeodomain (PHD) finger that specifically recognizes histone H3 trimethylated on lysine 4, and exhibits an even stronger preference for binding H3 that is concurrently trimethylated on lysine 4 and ..
  57. Aging and Antipsychotic Efficacy - Epigenetic Mechanisms
    Hongxin Dong; Fiscal Year: 2013
    ..induced c-Fos expression in the nucleus accumbens of aged mice, as well decreased acetylation of histone H3 lysine residue 27 (H3K27) on the c-fos promoter...
  58. Molecular Control of Brown Adipose Cell Fate and Energy Metabolism
    Shingo Kajimura; Fiscal Year: 2013
    ..We identified a lysine methyltransferase, EHMT1 as a critical component of the PRDM16 transcriptional complex...
  59. De novo establishment of Polycomb-group-mediated repression
    Richard S Jones; Fiscal Year: 2010
    ..Elements (PREs) and recruit additional PcG protein complexes, such as PRC2, which in turn methylates histone H3 at lysine 27 (H3K27me3), facilitating recruitment of PRC1...
  60. Rigid-rod Peptides as Nanocarriers for Delivery of Cancer Drugs
    Katarzyna Slowinska; Fiscal Year: 2013
    ..at developing peptide sequences to form water soluble model prodrugs based on PTX attached to the peptide's lysine group via succinylic link at C2'-OH position...
  61. Protein methyltransferases as transcriptional coregulators
    Michael R Stallcup; Fiscal Year: 2013
    ..In particular, di- and trimethylation of lysine 9 of histone H3 (H3 K9) in gene promoters has been associated with gene repression...
  62. Cancer epigenetics: Understanding histone methylation in leukemia stem cells
    Gang Greg Wang; Fiscal Year: 2011
    ..which targets molecular players that regulate a specific chromatin modification- histone H3 tri-methylated at lysine 4 (abbreviated as H3K4Me3)...
  63. Epigenetic regulation of viral infection and replication by periodontal bacteria
    Fengchun Ye; Fiscal Year: 2013
    ..Previous studies suggest that lysine acetylation of cytoskeleton actins and microtubule plays important roles in viral entry and intracellular ..
  64. MOLECULAR MECHANISMS OF OXIDATION OF LDL
    Sampath Parthasarathy; Fiscal Year: 1999
    ..A number of peptides from apo B and albumin, especially from lysine-rich regions of these proteins, will be tested for their ability to form fluorescent products...
  65. Histone Deacetylases and reversible acetylation in signaling and disease
    JOHN DENU; Fiscal Year: 2009
    ..Over the last several years, emerging evidence suggests that acetylation of protein lysine residues may rival reversible phosphorylation as a major regulatory mechanism of cellular pathways...
  66. AGING AND ALZHEIMER DEMENTIA--ROLE OF FIBROUS PROTEINS
    Shu Hui Yen; Fiscal Year: 2000
    ..phosphorylation, PHF-tau differs from normal tau in its increased content of D-aspartate and decreased content of lysine residues...
  67. HK polymers as transfection reagents for siRNA delivery
    Frank Xie; Fiscal Year: 2005
    We are proposing this project to develop and commercialize a novel, histidine/lysine (HK) polymer based, siRNA transfection reagent...
  68. BIOLOGICAL ROLE OF LYSYL OXIDASE-2 IN MATRIX DEVELOPMENT
    Ian Hornstra; Fiscal Year: 1999
    ..Lysyl oxidases are copper-dependent monoamine oxidase enzymes which even today are responsible for the lysine-derived cross-links in collagen and elastin of all animal species...
  69. Improved Peptide-based Tumor Targeting Agents Using Phage Display
    Susan L Deutscher; Fiscal Year: 2010
    ..Current techniques to block this retention, including lysine or diuretic administration are marginally effective and have harmful side effects...
  70. A Novel Epigenetic Gene Silencing Technology
    MING MING contact ZHOU; Fiscal Year: 2010
    ..For instance, histone H3 lysine 4 methylation (H3K4me) directed by the Trithorax group complex signifies for gene activation, whereas H3 lysine 27 ..
  71. Nutritional Intervention for Age-Related Muscular Function and Strength Losses
    John A Rathmacher; Fiscal Year: 2010
    ..We have recently demonstrated that the combination of HMB with two other amino acids, arginine and lysine, was effective in increasing muscle mass in elderly men and women during a year-long prospective study...
  72. NUTRITIONAL MODIFICATION OF CANCER CELL GROWTH
    CRAIG BYUS; Fiscal Year: 1990
    ..These specific amino acids are ornithine, arginine, and lysine. Recent studies in this laboratory have implicated arginine and ornithine as potential regulators of polyamine ..
  73. Structure and Mechanism of Pathogen SET Domain HKMTs
    Ming Ming Zhou; Fiscal Year: 2009
    ..Histone lysine methylation is almost exclusively catalyzed by a large family of the SET domain histone lysine methyltransferases (..
  74. A Hypersensitive ER alpha Mutant in Breast Cancer Hormone Resistance
    Suzanne Aw Fuqua; Fiscal Year: 2012
    ..This mutation replaces arginine for lysine at residue 303 (K303R), which greatly enhances sensitivity to very low levels of estrogen...
  75. Gene Delivery of P53 in a Tumor-bearing Mouse Model
    ARCHIBALD MIXSON; Fiscal Year: 2007
    ..Recently our laboratory has developed linear and branched HK (histidine-lysine) polymers that in combination with liposomes significantly increased transfection in the presence or absence of ..