Influenza A virus (A/Goose/Guangdong/1/96(H5N1))

Summary

Alias: influenza A virus (A/goose/Guangdong/1/96/(H5N1))

Top Publications

  1. doi Effect of receptor binding domain mutations on receptor binding and transmissibility of avian influenza H5N1 viruses
    Taronna R Maines
    Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road, MS G16, Atlanta, GA 30333, USA
    Virology 413:139-47. 2011
  2. pmc Transmission of influenza virus in a mammalian host is increased by PB2 amino acids 627K or 627E/701N
    John Steel
    Department of Microbiology, Mount Sinai School of Medicine, New York, NY, USA
    PLoS Pathog 5:e1000252. 2009
  3. doi Influenza A virus protein PB1-F2 exacerbates IFN-beta expression of human respiratory epithelial cells
    Ronan Le Goffic
    Unite de Virologie et Immunologie Moleculaires, Unité de Recherche 892 Institut National de la Recherche Agronomique, Domaine de Vilvert, Jouy en Josas, France
    J Immunol 185:4812-23. 2010
  4. doi PB1-mediated virulence attenuation of H5N1 influenza virus in mice is associated with PB2
    Jing Li
    State Key Laboratory of Pathogens and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, PR China
    J Gen Virol 92:1435-44. 2011
  5. pmc Differential contribution of PB1-F2 to the virulence of highly pathogenic H5N1 influenza A virus in mammalian and avian species
    Mirco Schmolke
    Department of Microbiology, Mount Sinai School of Medicine, New York, New York, United States of America
    PLoS Pathog 7:e1002186. 2011
  6. pmc The influenza A virus protein PB1-F2: killing two birds with one stone?
    Zsuzsanna T Varga
    Department of Microbiology, Mount Sinai School of Medicine, New York, NY, USA
    Virulence 2:542-6. 2011
  7. doi Adaptive mutations in NEP compensate for defective H5N1 RNA replication in cultured human cells
    Benjamin Mänz
    Department of Virology, Institute for Medical Microbiology and Hygiene, University of Freiburg, 79104, Germany
    Nat Commun 3:802. 2012
  8. doi Crystal structure of an avian influenza polymerase PA(N) reveals an endonuclease active site
    Puwei Yuan
    National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China
    Nature 458:909-13. 2009
  9. doi Crystal structure of the polymerase PA(C)-PB1(N) complex from an avian influenza H5N1 virus
    Xiaojing He
    National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China
    Nature 454:1123-6. 2008
  10. pmc The NS1 gene contributes to the virulence of H5N1 avian influenza viruses
    Zejun Li
    Harbin Veterinary Research Institute, CAAS, 427 Maduan Street, Harbin 150001, People s Republic of China
    J Virol 80:11115-23. 2006

Scientific Experts

  • Yusuke S Kato
  • Pierre Louis Hervé
  • Tony Velkov
  • Molvibha Vongsakul
  • Kelly K Lee
  • Qu zhi Wang
  • Jin xue Long
  • Angeline PC Lim
  • X Xu
  • James Lear
  • Anne Moscona
  • Yasuo Suzuki
  • Olga V Chervyakova
  • DEBI NAYAK
  • Ronan Le Goffic
  • Siba K Samal
  • Gennadi Glinsky
  • Yoshihiro Kawaoka
  • Hualan Chen
  • Shufang Fan
  • Hassan Zaraket
  • Jing Li
  • Weizhong Li
  • John Steel
  • Hans Dieter Klenk
  • Volker Czudai-Matwich
  • Linda Brunotte
  • Gülsah Gabriel
  • Wei Sun
  • Qingyu Zhu
  • Adolfo Garcia-Sastre
  • Wenjun Song
  • Zheng Li
  • Zsuzsanna T Varga
  • George F Gao
  • Peter Palese
  • Yumiko Matsuoka
  • Zhiyong Lou
  • Mark Bartlam
  • Xuemei Li
  • Zihe Rao
  • Yingfang Liu
  • Xiaojing He
  • Sean T Smrt
  • Chun Yu Zhu
  • Reina Yamaji
  • Shijuan Gao
  • Xiangwu Ju
  • Isabel Wendel
  • Mikhail Matrosovich
  • V Karthick
  • Likai Tan
  • Chunfeng Li
  • Shamika Danzy
  • Guomin Qian
  • Aleksandar Antanasijevic
  • Gabriele Neumann
  • Catherine A Macken
  • Martin Schwemmle
  • Amber M Smith
  • Zhuo Li
  • Olivier Leymarie
  • Chunyu Zhu
  • Chih Heng Huang
  • Peter Reuther
  • Quanjiao Chen
  • Charles J Russell
  • Holly Shelton
  • Xiao Jian Zhang
  • Olga A Bridges
  • Shinya Yamada
  • Masaya Miyazaki
  • Xiaoping Kang
  • Xiaoyan Wu
  • Yinhui Yang
  • Yi Hu
  • Balaji Manicassamy
  • Rachel E Johnson
  • Eefje J A Schrauwen
  • Amorsolo L Suguitan
  • Yi Zhang
  • Dong Jiang Tang
  • Wentian Chen
  • Chengjun Li
  • Jad Maamary
  • Benjamin Mänz
  • Tao Deng
  • Kwok Yung Yuen
  • Pui Wang
  • Honglin Chen

Detail Information

Publications89

  1. doi Effect of receptor binding domain mutations on receptor binding and transmissibility of avian influenza H5N1 viruses
    Taronna R Maines
    Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road, MS G16, Atlanta, GA 30333, USA
    Virology 413:139-47. 2011
    ....
  2. pmc Transmission of influenza virus in a mammalian host is increased by PB2 amino acids 627K or 627E/701N
    John Steel
    Department of Microbiology, Mount Sinai School of Medicine, New York, NY, USA
    PLoS Pathog 5:e1000252. 2009
    ..Thus, our data show that PB2 amino acids 627 and 701 are determinants of mammalian inter-host transmission in diverse virus backgrounds...
  3. doi Influenza A virus protein PB1-F2 exacerbates IFN-beta expression of human respiratory epithelial cells
    Ronan Le Goffic
    Unite de Virologie et Immunologie Moleculaires, Unité de Recherche 892 Institut National de la Recherche Agronomique, Domaine de Vilvert, Jouy en Josas, France
    J Immunol 185:4812-23. 2010
    ....
  4. doi PB1-mediated virulence attenuation of H5N1 influenza virus in mice is associated with PB2
    Jing Li
    State Key Laboratory of Pathogens and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, PR China
    J Gen Virol 92:1435-44. 2011
    ..However, the role of the N terminus of PB2 in virulence attenuation in mice remains unclear...
  5. pmc Differential contribution of PB1-F2 to the virulence of highly pathogenic H5N1 influenza A virus in mammalian and avian species
    Mirco Schmolke
    Department of Microbiology, Mount Sinai School of Medicine, New York, New York, United States of America
    PLoS Pathog 7:e1002186. 2011
    ..Our data could explain why PB1-F2 is conserved in avian influenza virus isolates and only impacts pathogenicity in mammals when containing certain amino acid motifs such as the rare N66S polymorphism...
  6. pmc The influenza A virus protein PB1-F2: killing two birds with one stone?
    Zsuzsanna T Varga
    Department of Microbiology, Mount Sinai School of Medicine, New York, NY, USA
    Virulence 2:542-6. 2011
    ..e. interferon antagonism, both in vitro and in the mouse model. Here, we discuss a hypothesis for a possible molecular link between the pro-apoptotic and anti-interferon functions of PB1-F2...
  7. doi Adaptive mutations in NEP compensate for defective H5N1 RNA replication in cultured human cells
    Benjamin Mänz
    Department of Virology, Institute for Medical Microbiology and Hygiene, University of Freiburg, 79104, Germany
    Nat Commun 3:802. 2012
    ..In conclusion, when crossing the species barrier, avian influenza viruses acquire adaptive mutations in nuclear export protein to escape restricted viral genome replication in mammalian cells...
  8. doi Crystal structure of an avian influenza polymerase PA(N) reveals an endonuclease active site
    Puwei Yuan
    National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China
    Nature 458:909-13. 2009
    ..The high conservation of this endonuclease active site among influenza strains indicates that PA(N) is an important target for the design of new anti-influenza therapeutics...
  9. doi Crystal structure of the polymerase PA(C)-PB1(N) complex from an avian influenza H5N1 virus
    Xiaojing He
    National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China
    Nature 454:1123-6. 2008
    ....
  10. pmc The NS1 gene contributes to the virulence of H5N1 avian influenza viruses
    Zejun Li
    Harbin Veterinary Research Institute, CAAS, 427 Maduan Street, Harbin 150001, People s Republic of China
    J Virol 80:11115-23. 2006
    ....
  11. pmc U4 at the 3' UTR of PB1 segment of H5N1 influenza virus promotes RNA polymerase activity and contributes to viral pathogenicity
    Wei Sun
    State Key Laboratory of Pathogens and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, PR China
    PLoS ONE 9:e93366. 2014
    ..In this way, these data showed the importance of untranslated regions of H5N1 influenza virus to pathogenicity. ..
  12. pmc Neuraminidase of Influenza A Virus Binds Lysosome-Associated Membrane Proteins Directly and Induces Lysosome Rupture
    Xiangwu Ju
    State Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, and Department of Biochemistry and Molecular Biology, Peking Union Medical College, Tsinghua University, Beijing, China
    J Virol 89:10347-58. 2015
    ..Our findings not only reveal a novel role of NA in the early stage of the H5N1 influenza virus life cycle but also elucidate the molecular mechanism of lysosomal rupture crucial for influenza virus induced cell death...
  13. doi Identification of NS1 domains of avian H5N1 influenza virus which influence the interaction with the NOLC1 protein
    Chun Yu Zhu
    Key Laboratory of Animal Resource and Epidemic Disease Prevention of Liaoning Province, College of Life Science of Liaoning University, Shenyang, 110036, People s Republic of China
    Virus Genes 50:238-44. 2015
    ..This study lays the foundation for further research aiming at furthering our understanding of the interaction between NS1 and host cells...
  14. pmc The avian-origin PB1 gene segment facilitated replication and transmissibility of the H3N2/1968 pandemic influenza virus
    Isabel Wendel
    Institute of Virology, Philipps University, Marburg, Germany
    J Virol 89:4170-9. 2015
    ..Our data suggest that the avian PB1 segment of the 1968 pandemic virus served to enhance viral growth and transmissibility, likely by enhancing activity of the viral polymerase complex...
  15. pmc Mammalian adaptive mutations of the PA protein of highly pathogenic avian H5N1 influenza virus
    Reina Yamaji
    Division of Virology, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, Tokyo, Japan
    J Virol 89:4117-25. 2015
    ..Intriguingly, PA-241Y, which 36285 encodes, is conserved in more than 90% of human seasonal H1N1 viruses, suggesting that PA-241Y contributes to virus adaptation to human lung cells and mammalian hosts...
  16. doi The amino-terminal region of the neuraminidase protein from avian H5N1 influenza virus is important for its biosynthetic transport to the host cell surface
    Guomin Qian
    Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China
    Vet J 202:612-7. 2014
    ..The results of the study reveal that amino acid residues 7-15 of H5N1 NA are critical for its biosynthetic transport to the host cell surface...
  17. pmc Integrating computational modeling and functional assays to decipher the structure-function relationship of influenza virus PB1 protein
    Chunfeng Li
    1 Center of Systems Medicine, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100005, China 2 Suzhou Institute of Systems Medicine, Suzhou 215123, China
    Sci Rep 4:7192. 2014
    ..Therefore, this study has not only deepened our understanding of structure-function relationship of PB1, but also promoted the development of novel therapeutics against influenza virus. ..
  18. pmc Molecular signatures of virulence in the PB1-F2 proteins of H5N1 influenza viruses
    Amber M Smith
    Department of Infectious Diseases, St Jude Children s Research Hospital, 262 Danny Thomas Place, Memphis, TN 38105, USA
    Virus Res 178:146-50. 2013
    ..Surveillance efforts should include sequencing of the PB1 gene segment and analysis for these molecular signatures to allow for the potential prioritization of resources during pandemic planning. ..
  19. doi Adaptive mutations in the H5N1 polymerase complex
    Gülsah Gabriel
    Heinrich Pette Institute, Leibniz Institute for Experimental Virology, Hamburg, Germany
    Virus Res 178:53-62. 2013
    ..Numerous other adaptive mutations, some of which compensate for the lack of PB2 E627K, have been observed in PB2 as well as in the polymerase subunit PB1, the nucleoprotein NP, and the nuclear export protein NEP (NS2). ..
  20. doi Insight into the oseltamivir resistance R292K mutation in H5N1 influenza virus: a molecular docking and molecular dynamics approach
    V Karthick
    Bioinformatics Division, School of Bio Sciences and Technology, VIT University, Vellore, 632014, Tamil Nadu, India
    Cell Biochem Biophys 68:291-9. 2014
    ..It is believed that this study provides valuable guidance for the resistance management of oseltamivir and designing of more potent antiviral inhibitor. ..
  21. doi Caspase-1 deficient mice are more susceptible to influenza A virus infection with PA variation
    Chih Heng Huang
    Research Center for Emerging Viral Infections
    J Infect Dis 208:1898-905. 2013
    ..We previously reported that the 2009 pH1N1 PA enhanced the pathogenicity of seasonal H1N1. We examined the effects of the PA gene from the HPAI H5N1 following its introduction into currently circulating seasonal influenza viruses...
  22. doi NS1-binding protein abrogates the elevation of cell viability by the influenza A virus NS1 protein in association with CRKL
    Masaya Miyazaki
    Department of Cancer Pathology, Hokkaido University Graduate School of Medicine, N15W7, Kita ku, Sapporo 060 8638, Japan
    Biochem Biophys Res Commun 441:953-7. 2013
    ..In addition, an alternative role of adaptor protein CRKL in association with NS1 and NS1-BP during influenza A virus infection is demonstrated. ..
  23. ncbi [Construction and biological characteristics of H5N1 avian influenza viruses with different patterns of the glycosylation sites in HA protein]
    Xiao Jian Zhang
    Key Laboratory of Animal Infectious Disease, Ministry of Agriculture, Yangzhou University, Yangzhou 225009, China
    Bing Du Xue Bao 29:495-9. 2013
    ..All viruses remained high pathogenicity to SPF chickens. Therefore, the growth of AIV can be affected by changes of glycosylation sites in HA protein, by which the effect is variable in different cells...
  24. pmc The influenza hemagglutinin fusion domain is an amphipathic helical hairpin that functions by inducing membrane curvature
    Sean T Smrt
    From the Department of Chemistry, University of Illinois, Chicago, Illinois 60607
    J Biol Chem 290:228-38. 2015
    ..The inverted wedge shape of HAfp23 is likely to play a crucial role in the initial stages of membrane fusion by stabilizing negative curvature in the fusion stalk. ..
  25. pmc The nuclear export protein of H5N1 influenza A viruses recruits Matrix 1 (M1) protein to the viral ribonucleoprotein to mediate nuclear export
    Linda Brunotte
    From the Institute for Virology, University Medical Center Freiburg, Hermann Herder Str 11, 79104 Freiburg, Germany and
    J Biol Chem 289:20067-77. 2014
    ..On the basis of our data, we propose a new model for the assembly of the nuclear export complex of Influenza A vRNPs. ..
  26. pmc PB2 mutations D701N and S714R promote adaptation of an influenza H5N1 virus to a mammalian host
    Volker Czudai-Matwich
    Institute of Virology, Philipps University, Marburg, Germany
    J Virol 88:8735-42. 2014
    ..These observations indicate that PB2 mutation S714R, in combination with the mammalian signature at position 701, has the potential to promote the adaptation of an H5N1 virus to a mammalian host...
  27. doi Addition of N-glycosylation sites on the globular head of the H5 hemagglutinin induces the escape of highly pathogenic avian influenza A H5N1 viruses from vaccine-induced immunity
    Pierre Louis Hervé
    Institut Pasteur, Unité de Génétique Moléculaire des Virus à ARN, 25 28 Rue du Docteur Roux, F 75015 Paris, France CNRS UMR 3569, 25 28 Rue du Docteur Roux, F 75015 Paris, France Université Paris Diderot, Sorbonne, Paris Cité, EA 302, 25 28 Rue du Docteur Roux, Paris, France Electronic address
    Virology 486:134-45. 2015
    ..Seven H5N1 mutants were produced by adding glycosites on H5. The most glycosylated virus escaped from neutralizing antibodies, in vitro and in vivo. Furthermore, a single additional glycosite was responsible for this escape. ..
  28. pmc Mutagenesis studies of the H5 influenza hemagglutinin stem loop region
    Aleksandar Antanasijevic
    From the Department of Biochemistry and Molecular Genetics, University of Illinois, Chicago, Illinois 60607
    J Biol Chem 289:22237-45. 2014
    ..Together these studies underscore the importance of the stem loop region to HA function and suggest potential sites for therapeutic intervention of influenza entry. ..
  29. pmc Amino acid substitutions in PB1 of avian influenza viruses influence pathogenicity and transmissibility in chickens
    Yasushi Suzuki
    Influenza and Prion Disease Research Center, National Institute of Animal Health, National Agriculture and Food Research Organization NARO, Tsukuba, Ibaraki, Japan
    J Virol 88:11130-9. 2014
    ..These studies demonstrate that increased polymerase activity correlates directly with enhanced pathogenicity, while decreased polymerase activity does not always correlate with pathogenicity and requires further analysis...
  30. pmc Mutations to PB2 and NP proteins of an avian influenza virus combine to confer efficient growth in primary human respiratory cells
    Shamika Danzy
    Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, Georgia, USA
    J Virol 88:13436-46. 2014
    ....
  31. pmc The K526R substitution in viral protein PB2 enhances the effects of E627K on influenza virus replication
    Wenjun Song
    1 State Key Laboratory for Emerging Infectious Diseases, Department of Microbiology, and the Research Center of Infection and Immunology, The University of Hong Kong, Hong Kong SAR, China 2 The Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Zhejiang University, Hangzhou 310003, China
    Nat Commun 5:5509. 2014
    ..PB2-K526R interacts with nuclear export protein and our results suggest that it contributes to enhance replication for certain influenza virus subtypes, particularly in combination with 627K. ..
  32. pmc A combination of HA and PA mutations enhances virulence in a mouse-adapted H6N6 influenza A virus
    Likai Tan
    Key Laboratory of Veterinary Vaccine Innovation of the Ministry of Agriculture, College of Veterinary Medicine, South China Agricultural University, Guangzhou, People s Republic of China
    J Virol 88:14116-25. 2014
    ..This suggests that these substitutions can compensate for the lack of PB2(627K) and modulate virulence, revealing a new determinant of pathogenicity for H6N6 viruses in mice, which might also pose a threat to human health...
  33. doi Novel residues in avian influenza virus PB2 protein affect virulence in mammalian hosts
    Shufang Fan
    Influenza Research Institute, School of Veterinary Medicine, University of Wisconsin Madison, Madison, Wisconsin 53711, USA
    Nat Commun 5:5021. 2014
    ..Importantly, H5N1 viruses bearing the former three PB2 residues have circulated widely in recent years in avian species in nature. ..
  34. pmc Interaction of NS2 with AIMP2 facilitates the switch from ubiquitination to SUMOylation of M1 in influenza A virus-infected cells
    Shijuan Gao
    CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China
    J Virol 89:300-11. 2015
    ..Collectively, our results reveal a new mechanism of AIMP2 mediation of influenza virus replication...
  35. doi The antigenic architecture of the hemagglutinin of influenza H5N1 viruses
    Tony Velkov
    Drug Delivery, Disposition and Dynamics, Monash Institute of Pharmaceutical Sciences, Monash University, 381 Royal Parade, Parkville, Victoria 3052, Australia Electronic address
    Mol Immunol 56:705-19. 2013
    ....
  36. pmc Increased acid stability of the hemagglutinin protein enhances H5N1 influenza virus growth in the upper respiratory tract but is insufficient for transmission in ferrets
    Hassan Zaraket
    Department of Infectious Diseases, St Jude Children s Research Hospital, Memphis, Tennessee, USA
    J Virol 87:9911-22. 2013
    ....
  37. pmc Efficacy of parainfluenza virus 5 mutants expressing hemagglutinin from H5N1 influenza A virus in mice
    Zhuo Li
    Department of Infectious Diseases, University of Georgia College of Veterinary Medicine, Athens, Georgia, USA
    J Virol 87:9604-9. 2013
    ..These results suggest that PIV5ΔSH is a better vaccine vector than wild-type PIV5. ..
  38. pmc Kinetic characterization of PB1-F2-mediated immunopathology during highly pathogenic avian H5N1 influenza virus infection
    Olivier Leymarie
    Unite de Virologie et Immunologie Moleculaires, UR 892 INRA, Domaine de Vilvert, Jouy en Josas, France
    PLoS ONE 8:e57894. 2013
    ....
  39. pmc The pH of activation of the hemagglutinin protein regulates H5N1 influenza virus replication and pathogenesis in mice
    Hassan Zaraket
    Department of Infectious Diseases, St Jude Children s Research Hospital, Memphis, Tennessee, USA
    J Virol 87:4826-34. 2013
    ..4. Identification of the HA activation pH as a host-specific infectivity factor is expected to aid in the surveillance and risk assessment of currently circulating H5N1 influenza viruses...
  40. pmc Synergistic effect of the PDZ and p85β-binding domains of the NS1 protein on virulence of an avian H5N1 influenza A virus
    Shufang Fan
    Influenza Research Institute, Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin Madison, Madison, Wisconsin, USA
    J Virol 87:4861-71. 2013
    ..These findings suggest a functional interplay between the mutations at NS1-138 and NS1-229 that results in a synergistic effect on influenza virulence...
  41. pmc NA proteins of influenza A viruses H1N1/2009, H5N1, and H9N2 show differential effects on infection initiation, virus release, and cell-cell fusion
    Quanjiao Chen
    State Key Laboratory of Virology, Center for Emerging Infectious Diseases, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, Hubei, China
    PLoS ONE 8:e54334. 2013
    ..These phenomena might be partially due to NA proteins' different specificity to α2-3/2-6-sialylated carbohydrate chains, but the exact mechanism remains to be explored...
  42. pmc Mutations in haemagglutinin that affect receptor binding and pH stability increase replication of a PR8 influenza virus with H5 HA in the upper respiratory tract of ferrets and may contribute to transmissibility
    Holly Shelton
    Division of Infectious Diseases, Imperial College London, St Mary s Campus, London, UK
    J Gen Virol 94:1220-9. 2013
    ....
  43. pmc Interaction of avian influenza virus NS1 protein and nucleolar and coiled-body phosphoprotein 1
    Chunyu Zhu
    Key Laboratory of Animal Resource and Epidemic Disease Prevention, Life Science School of Liaoning University, Shenyang 110036, China
    Virus Genes 46:287-92. 2013
    ..The results demonstrated a positive interaction between a viral NS1 and NOLC1 of the host cells, and provided a new target for drug screening...
  44. pmc The multibasic cleavage site in H5N1 virus is critical for systemic spread along the olfactory and hematogenous routes in ferrets
    Eefje J A Schrauwen
    National Influenza Center and Department of Virology, Erasmus Medical Center, Rotterdam, The Netherlands
    J Virol 86:3975-84. 2012
    ..In conclusion, HPAI H5N1 virus can spread systemically via two different routes, olfactory and hematogenous, in ferrets. This systemic spread was dependent on the presence of the MBCS in HA...
  45. pmc The evolutionary pattern of glycosylation sites in influenza virus (H5N1) hemagglutinin and neuraminidase
    Wentian Chen
    Laboratory for Functional Glycomics, College of Life Sciences, Northwest University, Xi an, People s Republic of China
    PLoS ONE 7:e49224. 2012
    ....
  46. pmc A single residue substitution in the receptor-binding domain of H5N1 hemagglutinin is critical for packaging into pseudotyped lentiviral particles
    Dong Jiang Tang
    HKU Pasteur Research Centre, The University of Hong Kong, Hong Kong, Special Administrative Region, People s Republic of China
    PLoS ONE 7:e43596. 2012
    ..Here we show that H5-HAs from different clades do not always give rise to efficient production of H5pp and the underlying mechanisms are addressed...
  47. doi Heterologous interactions between NS1 proteins from different influenza A virus subtypes/strains
    Weizhong Li
    Department of Microbiology and Immunology, Key Immunopathology Laboratory of Guangdong Province, Shantou University Medical College, Shantou 515041, China
    Sci China Life Sci 55:507-15. 2012
    ..4%-20.9% sequence diversity with NS11, NS51 and NS92. This indicates that the heterologous interactions between NS1 proteins from different influenza A virus subtypes/ strains may be a common event during co-infection...
  48. pmc Permissible variation in the 3' non-coding region of the haemagglutinin genome segment of the H5N1 candidate influenza vaccine virus NIBRG-14 [corrected]
    Rachel E Johnson
    Division of Virology, National Institute for Biological Standards and Control, Health Protection Agency, Blanche Lane, Potters Bar, Hertfordshire, United Kingdom
    PLoS ONE 7:e36241. 2012
    ..These findings may serve to assure the influenza vaccine community that generation of CVVs using best-guess NCR sequences, based on sequence alignments, are likely to produce robust viruses...
  49. pmc A single amino acid at the hemagglutinin cleavage site contributes to the pathogenicity and neurovirulence of H5N1 influenza virus in mice
    Yi Zhang
    Key Laboratory of Animal Epidemiology and Zoonosis, Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing, China
    J Virol 86:6924-31. 2012
    ..Our results demonstrate that an amino acid substitution at the P6 cleavage site alone could modulate the virulence of H5N1 in mice...
  50. pmc Attenuated influenza virus construct with enhanced hemagglutinin protein expression
    Jad Maamary
    Department of Microbiology, Mount Sinai School of Medicine, New York, New York, USA
    J Virol 86:5782-90. 2012
    ....
  51. ncbi The difference in IL-1beta , MIP-1alpha, IL-8 and IL-18 production between the infection of PMA activated U937 cells with recombinant vaccinia viruses inserted 2004 H5N1 influenza HA genes and NS genes
    Molvibha Vongsakul
    Department of Microbiology, Faculty of Science Mahidol University, Bangkok, Thailand
    Asian Pac J Allergy Immunol 29:349-56. 2011
    ..However, the role of each virus gene is unknown. To elaborate the function of each virus gene, the recombinant vaccinia virus inserted HA and NS gene from the 2004 H5N1 virus were used in the study...
  52. pmc The multibasic cleavage site of the hemagglutinin of highly pathogenic A/Vietnam/1203/2004 (H5N1) avian influenza virus acts as a virulence factor in a host-specific manner in mammals
    Amorsolo L Suguitan
    Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA
    J Virol 86:2706-14. 2012
    ..Thus, the contribution of H5 HA MBS to the virulence of the HPAI H5N1 virus varies among mammalian hosts and is most significant in mice and ferrets and less remarkable in nonhuman primates...
  53. pmc Capturing a fusion intermediate of influenza hemagglutinin with a cholesterol-conjugated peptide, a new antiviral strategy for influenza virus
    Kelly K Lee
    Department of Medicinal Chemistry and Biomolecular Structure and Design Program, University ofWashington, Seattle, Washington 98195, USA
    J Biol Chem 286:42141-9. 2011
    ..These results provide proof of concept for an antiviral strategy that is applicable to intracellularly fusing viruses, including known and emerging viral pathogens...
  54. doi Identification of amino acids in highly pathogenic avian influenza H5N1 virus hemagglutinin that determine avian influenza species specificity
    Zheng Li
    The Department of Immunology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and School of Basic Medicine, Peking Union Medical College, 5 Dong Dan San Tiao, Beijing 100005, China
    Arch Virol 156:1803-12. 2011
    ..The mutation is the result of adaptation caused by the receptor. Our results suggest that continuing occurrence of these two types of mutations made the variants persist in the new host species...
  55. pmc The nucleoprotein and matrix protein segments of H5N1 influenza viruses are responsible for dominance in embryonated eggs
    Kenta Shimizu
    Division of Virology, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, Tokyo, Japan
    J Gen Virol 92:1645-9. 2011
    ..These results suggest roles for these viral proteins in influenza virus dominance...
  56. doi Free energy simulations reveal a double mutant avian H5N1 virus hemagglutinin with altered receptor binding specificity
    Payel Das
    Computational Biology Center, IBM Thomas J Watson Research Center, Yorktown Heights, New York 10598, USA
    J Comput Chem 30:1654-63. 2009
    ..These large scale molecular simulations on single and double mutants thus provide new insights into our understanding toward human adaptation of the avian H5N1 virus...
  57. pmc The host-dependent interaction of alpha-importins with influenza PB2 polymerase subunit is required for virus RNA replication
    Patricia Resa-Infante
    Centro Nacional de Biotecnología CSIC Darwin 3, Campus de Cantoblanco, Madrid, Spain
    PLoS ONE 3:e3904. 2008
    ....
  58. pmc Neuraminidase stalk length and additional glycosylation of the hemagglutinin influence the virulence of influenza H5N1 viruses for mice
    Yumiko Matsuoka
    Influenza Division, Centers for Disease Control and Prevention, Atlanta, Georgia, USA
    J Virol 83:4704-8. 2009
    ..The presence of additional HA glycosylation sites had less of an effect on virulence than did NA stalk length. The short-stalk NA of H5N1 viruses circulating in Asia may contribute to virulence in humans...
  59. ncbi [Identification of peptide mimotopes of an abroad-spectrum neutralizing epitope of highly pathogenic avian influenza hemagglutinin]
    Hui Juan Song
    National Institute of Diagnostics and Vaccine Development in Infectious Diseases and School of Life Sciences, Xiamen University, Xiamen 361005, China
    Bing Du Xue Bao 24:421-6. 2008
    ..The antiserum induced by HBc-T123 intensively stained on SF21 cells infected by recombinant baculovirus containing HA gene of YU22 virus, indicating the production of cross-reactive antibody to H5N1 HA...
  60. doi Identification of amino acid substitutions in mutated peptides of nucleoprotein from avian influenza virus
    Ning Liu
    Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, Hong Kong, SAR, China
    Talanta 78:1492-6. 2009
    ..The MS/MS analyses allowed the confident determination of the three mutated amino acid residues F313Y, I194V and V408I/L in the mutated peptides of LLQNSQVYSLIRPNENPAHK, GVGTMVMELVR and ASAGQI/LSVQPTFSVQR, respectively...
  61. ncbi Genetic characterization of the pathogenic influenza A/Goose/Guangdong/1/96 (H5N1) virus: similarity of its hemagglutinin gene to those of H5N1 viruses from the 1997 outbreaks in Hong Kong
    X Xu
    Influenza Branch, Division of Viral Diseases, Center for Disease Control and Prevention, Atlanta, Georgia, 30333, USA
    Virology 261:15-9. 1999
    ..These data suggest that the H5N1 viruses isolated from the Hong Kong outbreaks derived their HA genes from a virus similar to the A/Goose/Guangdong/1/96 virus or shared a progenitor with this goose pathogen...
  62. ncbi [Influence of avian influenza virus NS1 protein on the expression of IP-10 in BEAS-2B cells]
    Xiao Jun Jia
    Department of Animal Medicine, Northwest Agriculture and Forestry University, Yangling 712100, China
    Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi 22:183-5. 2008
    ..To investigate the influence of avian influenza virus (AIV) NS1 protein on the expression of interferon-inducible protein 10 (IP-10)...
  63. pmc Neutralizing human monoclonal antibody against H5N1 influenza HA selected from a Fab-phage display library
    Angeline P C Lim
    Defence Medical and Environmental Research Institute, DSO National Laboratories, 27 Medical Dr, 117510, Singapore
    Virol J 5:130. 2008
    ....
  64. pmc Structure of the influenza virus A H5N1 nucleoprotein: implications for RNA binding, oligomerization, and vaccine design
    Andy Ka Leung Ng
    Department of Biochemistry and Center for Protein Science and Crystallography, The Chinese University of Hong Kong, Shatin, Hong Kong SAR, China
    FASEB J 22:3638-47. 2008
    ..Our study of H5N1 NP provides insight into the oligomerization interface and the RNA-binding groove, which are attractive drug targets, and it identifies the epitopes that might be used for universal vaccine development...
  65. doi Virulence of H5N1 avian influenza virus enhanced by a 15-nucleotide deletion in the viral nonstructural gene
    Jin xue Long
    Animal Infectious Disease Laboratory, School of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu 225009, PR China
    Virus Genes 36:471-8. 2008
    ..The results indicated that the 15-nucleotide deletion of NS gene from site 263 to 277 associated with D92E shift in NS1 protein contributes to the virulence increase of H5N1 viruses in chickens and mice...
  66. pmc NP, PB1, and PB2 viral genes contribute to altered replication of H5N1 avian influenza viruses in chickens
    Jamie L Wasilenko
    Southeast Poultry Research Laboratory, USDA ARS, 934 College Station Road, Athens, GA 30605, USA
    J Virol 82:4544-53. 2008
    ..While the pathogenesis of AIVs in chickens is clearly dependent on the interaction of multiple gene products, we have shown that single-gene reassortment events are sufficient to alter the virulence of AIVs in chickens...
  67. ncbi [Genetic analysis of NS1 fragment of human H5N1 influenza virus isolated in Anhui province and its expression in Escherichia coli]
    Cong sheng Cheng
    National Key Laboratory for Infectious Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China
    Wei Sheng Wu Xue Bao 47:418-22. 2007
    ..As a result, the work paves the way for further understanding the role of NSI in human H5N1 infection and development of new antiviral drugs against influenza virus...
  68. ncbi [Biological significance of amino acids deletion in NA stalk of H5N1 avian influenza virus]
    Qu zhi Wang
    Key Laboratory of Animal Infectious Diseases, Ministry of Agriculture, Yangzhou University, Yangzhou 225009, China
    Wei Sheng Wu Xue Bao 46:542-6. 2006
    ..The unique amino acids deletion in NA molecule of H5N1 may be associated with the adaptation of virus to terrestrial poultry or the increasing ability of interspecies transmission...
  69. ncbi [The deletion of nucleotides of NS gene from 263 to 277 of H5N1 increases viral virulence in chicken]
    Jin xue Long
    Key Laboratory of Animal Infectious Diseases of Ministry of Agriculture, Yangzhou University, Yangzhou 225009, China
    Wei Sheng Wu Xue Bao 46:301-5. 2006
    ..81. RWSN-m848 caused one of the ten chickens died and its' index was only 0.175. The results revealed that the deletion of nucleotides of NS gene from 263 to 277 sites increases H5N1 pathogenesis in chicken...
  70. pmc Dendritic cell activation by recombinant hemagglutinin proteins of H1N1 and H5N1 influenza A viruses
    Wen Chun Liu
    Institute of Biotechnology, National Tsing Hua University, Hsinchu 30013, Taiwan
    J Virol 84:12011-7. 2010
    ..The stimulation of mDCs by HA proteins of H1N1 and H5N1 was completely MyD88 dependent. These findings may provide useful information for the development of more-effective influenza vaccines...
  71. pmc Virulence determinants of avian H5N1 influenza A virus in mammalian and avian hosts: role of the C-terminal ESEV motif in the viral NS1 protein
    Florian Zielecki
    Division of Influenza Respiratory Viruses, Robert Koch Institut, Nordufer 20, 13353 Berlin, Germany
    J Virol 84:10708-18. 2010
    ..These findings demonstrate that a PDZ domain ligand sequence in NS1 contributes little to the virulence of H5N1 viruses in these hosts, and they indicate that this motif modulates viral replication in a strain- and host-dependent manner...
  72. pmc Influenza virus non-structural protein 1 (NS1) disrupts interferon signaling
    Danlin Jia
    Department of Immunology, University of Toronto, Toronto, Canada
    PLoS ONE 5:e13927. 2010
    ..The data suggest that NS1 can directly interfere with IFN signaling to enhance viral replication, but that treatment with IFN can nevertheless override these inhibitory effects to block H5N1 and H1N1 virus infections...
  73. pmc Differential effects of NS1 proteins of human pandemic H1N1/2009, avian highly pathogenic H5N1, and low pathogenic H5N2 influenza A viruses on cellular pre-mRNA polyadenylation and mRNA translation
    Denis E Kainov
    Institute of Immunology, Centre de Recherche Public Santé Laboratoire National de Santé, L 1011, Luxembourg
    J Biol Chem 286:7239-47. 2011
    ..Thus, we identified strain-specific differences between influenza virus NS1 proteins in pre-mRNA polyadenylation and mRNA translation...
  74. pmc The PA protein directly contributes to the virulence of H5N1 avian influenza viruses in domestic ducks
    Jiasheng Song
    Harbin Veterinary Research Institute, CAAS, Harbin 150001, People s Republic of China
    J Virol 85:2180-8. 2011
    ..Our results provide strong evidence that the polymerase PA subunit is a virulence factor for H5N1 AIVs in ducks...
  75. doi Molecular and genetic analysis of NS gene from high pathogenic strains of the avian influenza (H5N1) virus isolated in Kazakhstan
    Olga V Chervyakova
    Research Institute for Biological Safety Problems, RK ME and S Science Committee, Gvardeiskiy, Kordaiskiy rayon, Zhambylskaya oblast, 080409, Republic of Kazakhstan
    Gene 476:15-9. 2011
    ..The results of the analysis allow assuming that A/swan/Mangystau/3/2006 strain has been brought onto the territory of Kazakhstan from the European part of the continent along the Black Sea-Mediterranean flyway...
  76. pmc Nucleoside monophosphate complex structures of the endonuclease domain from the influenza virus polymerase PA subunit reveal the substrate binding site inside the catalytic center
    Cong Zhao
    Laboratory of Structural Biology, Room 201, New Life Sciences Building, Tsinghua University, Beijing 100084, China
    J Virol 83:9024-30. 2009
    ..The identification of this binding pocket opens a new avenue for anti-influenza drug discovery, targeting the cap-dependent endonuclease, in response to the worldwide threat of influenza...
  77. pmc Effect of neuraminidase inhibitor-resistant mutations on pathogenicity of clade 2.2 A/Turkey/15/06 (H5N1) influenza virus in ferrets
    Natalia A Ilyushina
    Division of Virology, Department of Infectious Diseases, St Jude Children s Research Hospital, Memphis, Tennessee, United States of America
    PLoS Pathog 6:e1000933. 2010
    ..There is a need for novel anti-influenza drugs that target different virus/host factors and can limit the emergence of resistance...
  78. pmc Effects of NS1 variants of H5N1 influenza virus on interferon induction, TNFalpha response and p53 activity
    Weizhong Li
    Department of Microbiology and Immunology, Key Immunopathology Laboratory of Guangdong Province, Shantou University Medical College, Shantou, China
    Cell Mol Immunol 7:235-42. 2010
    ..Our findings shed new light on the role of NS1 in the pathogenicity of H5N1 virus...
  79. doi Correlation between polymerase activity and pathogenicity in two duck H5N1 influenza viruses suggests that the polymerase contributes to pathogenicity
    Bo Wah Leung
    Sir William Dunn School of Pathology, University of Oxford, Oxford, UK
    Virology 401:96-106. 2010
    ..Overall, we suggest that the influenza polymerase is one of the determinants of pathogenicity of duck H5N1 viruses...
  80. ncbi Genomic analysis of pandemic (H1N1) 2009 reveals association of increasing disease severity with emergence of novel hemagglutinin mutations
    Gennadi V Glinsky
    Translational and Functional Genomics Laboratory, Department of Pathology and Laboratory Medicine, and Department of Surgery, Division of Urology, Albany Medical College, Ordway Cancer Center, Ordway Research Institute, Inc, Center for Medical Science, Albany, NY, USA
    Cell Cycle 9:958-70. 2010
    ....
  81. pmc Contributions of the avian influenza virus HA, NA, and M2 surface proteins to the induction of neutralizing antibodies and protective immunity
    Baibaswata Nayak
    Virginia Maryland Regional College of Veterinary Medicine, University of Maryland, College Park, Maryland, USA
    J Virol 84:2408-20. 2010
    ..Thus, there was no indication that M2 is immunogenic or protective. Furthermore, inclusion of NA in addition to HA in a vaccine preparation for chickens may not enhance the high level of protection provided by HA...
  82. pmc Glycans on influenza hemagglutinin affect receptor binding and immune response
    Cheng Chi Wang
    Genomics Research Center, Chemical Biology and Molecular Biophysics, Taiwan International Graduate Program, Academia Sinica, Taipei 115, Taiwan
    Proc Natl Acad Sci U S A 106:18137-42. 2009
    ..Thus, removal of structurally nonessential glycans on viral surface glycoproteins may be a very effective and general approach for vaccine design against influenza and other human viruses...
  83. doi Stable non-synonymous substitutions on NS gene (NS1 and NS2 proteins) of Qinghai Lake H5N1 influenza virus (Clade 2.2) after successive passages in Muscovy ducks
    Xiaohui Song
    Key Laboratory of Pathogenic Microbiology and Immunology of CAS, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China
    Sci China C Life Sci 52:847-53. 2009
    ..Although the exact functions of these mutations are not known, our results provide an important foundation for further understanding the characteristics of the Qinghai Lake isolates...
  84. doi Fine antigenic variation within H5N1 influenza virus hemagglutinin's antigenic sites defined by yeast cell surface display
    Jian Li
    The Institute of Immunology, PLA, Third Military Medical University, Chongqing, P R China
    Eur J Immunol 39:3498-510. 2009
    ..The results demonstrate the existence of immunodominant positions in the H5 HA protein, alteration of these residues might improve the immunogenicity of vaccine strains...
  85. ncbi Neuraminidase inhibitors for influenza
    Anne Moscona
    Department of Pediatrics, Weill Medical College of Cornell University, New York, NY 10021, USA
    N Engl J Med 353:1363-73. 2005
  86. ncbi Sialobiology of influenza: molecular mechanism of host range variation of influenza viruses
    Yasuo Suzuki
    Department of Biochemistry, University of Shizuoka School of Pharmaceutical Sciences, Shizuoka, Japan
    Biol Pharm Bull 28:399-408. 2005
    ....
  87. ncbi Assembly and budding of influenza virus
    Debi P Nayak
    Department of Microbiology, Immunology and Molecular Genetics, Jonsson Comprehensive Cancer Center, Molecular Biology Institute, David Geffen School of Medicine, UCLA, Los Angeles, CA 90095, USA
    Virus Res 106:147-65. 2004
    ..Elucidation of both viral and host factors involved in viral morphogenesis and budding may lead to the development of drugs interfering with the steps of viral morphogenesis and in disease progression...
  88. ncbi Computational studies of proton transport through the M2 channel
    Yujie Wu
    Department of Chemistry and Henry Eyring Center for Theoretical Chemistry, University of Utah, 315 S 1400 E Rm 2020, Salt Lake City, UT 84112 0850, USA
    FEBS Lett 552:23-7. 2003
    ..Computer simulations of an excess proton in the channel and computational studies of the His37/Trp41 conformations have provided insights into these structural and mechanism issues...
  89. ncbi Proton conduction through the M2 protein of the influenza A virus; a quantitative, mechanistic analysis of experimental data
    James D Lear
    Johnson Research Foundation, Department of Biochemistry and Biophysics, School of Medicine of the University of Pennsylvania, Philadelphia, PA 19104 6059, USA
    FEBS Lett 552:17-22. 2003
    ....

Research Grants4

  1. CLONING AND EXPRESSION OF INFLUENZA VIRAL RNA SEGMENTS
    DEBI NAYAK; Fiscal Year: 2005
    ..A detailed understanding of these processes will facilitate the rational development of antiviral agents which could interfere with one or more steps in virus assembly. ..
  2. INTERFERENCE BY DEFECTIVE INFLUENZA VIRUSES
    DEBI NAYAK; Fiscal Year: 2007
    ..abstract_text> ..
  3. Design of peptide entry inhibitors and delivery systems to target emerging henipa
    Anne Moscona; Fiscal Year: 2008
    ..abstract_text> ..
  4. Design of peptide entry inhibitors and delivery systems to target emerging henipa
    Anne Moscona; Fiscal Year: 2010
    ..abstract_text> ..