Genomes and Genes
Alias: "Sulfolobus islandicus" Zillig et al. 1994
Publications101 found, 100 shown here
- Halocins and sulfolobicins: the emerging story of archaeal protein and peptide antibioticsE M O'Connor
Department of Biological Sciences, Northern Arizona University, Flagstaff, AZ 86011 5640, USA
J Ind Microbiol Biotechnol 28:23-31. 2002..Finally, archaeocins also are produced by the hyperthermophile "Sulfolobus islandicus"...
- A novel interference mechanism by a type IIIB CRISPR-Cmr module in SulfolobusLing Deng
Archaea Centre, Department of Biology, University of Copenhagen, Ole Maaløes Vej 5, DK2200, Copenhagen N, Denmark
Mol Microbiol 87:1088-99. 2013..The archaeon Sulfolobus islandicus REY15A encodes three interference modules, one of type IA and two of type IIIB...
- Genetic determinants of PAM-dependent DNA targeting and pre-crRNA processing in Sulfolobus islandicusWenfang Peng
State Key Laboratory of Agricultural Microbiology and College of Life Science and Technology, Huazhong Agricultural University, Wuhan, China
RNA Biol 10:738-48. 2013..The crenarchaeon Sulfolobus islandicus encodes three distinct CRISPR interference modules, including a type IA system and two type IIIB systems: ..
- Reassortment of CRISPR repeat-spacer loci in Sulfolobus islandicusNicole L Held
Department of Microbiology, University of Illinois, Urbana Champaign, Urbana, IL, USA
Environ Microbiol 15:3065-76. 2013..To assess evolutionary dynamics of CRISPR loci, we have sampled a population of closely related Sulfolobus islandicus strains from Kamchatka, Russia at two time points, 10 years apart...
- Geographic divergence of "Sulfolobus islandicus" strains assessed by genomic analyses including electronic DNA hybridization confirms they are geovarsGuanghong Zuo
T life Research Center, Fudan University, Shanghai, 200433, China
Antonie Van Leeuwenhoek 105:431-5. 2014Ten well-annotated genomes of "Sulfolobus islandicus" strains from different geographic locations have been released at the NCBI database...
- Recombination shapes genome architecture in an organism from the archaeal domainDavid J Krause
Department of Microbiology, University of Illinois at Urbana Champaign
Genome Biol Evol 6:170-8. 2014..across the chromosomes of ten closely related sympatric strains of the thermoacidophilic archaeon Sulfolobus islandicus. Statistical analyses show that recombination varies across the genome and interacts with selection to ..
- Inter-viral conflicts that exploit host CRISPR immune systems of SulfolobusSusanne Erdmann
Archaea Centre, Department of Biology, University of Copenhagen, Ole Maaløes Vej 5, DK 2200 N, Copenhagen, Denmark
Mol Microbiol 91:900-17. 2014Infection of Sulfolobus islandicus REY15A with mixtures of different Sulfolobus viruses, including STSV2, did not induce spacer acquisition by the host CRISPR immune system...
- An archaeal CRISPR type III-B system exhibiting distinctive RNA targeting features and mediating dual RNA and DNA interferenceWenfang Peng
State Key Laboratory of Agricultural Microbiology and College of Life Science and Technology, Huazhong Agricultural University, 430070 Wuhan, China Archaea Centre, Department of Biology, University of Copenhagen, Ole Maaløes Vej 5, DK 2200 Copenhagen N, Denmark
Nucleic Acids Res 43:406-17. 2015..To investigate the in vivo mechanism of RNA interference by two type III-B systems (Cmr-α and Cmr-β) in Sulfolobus islandicus, a genetic assay was developed using plasmids carrying an artificial mini-CRISPR (AC) locus with a single ..
- [Archaeons--still unknown microorganisms]Magdalena Efenberger
Zakład Immunologii Doświadczalnej, Uniwersytet Medyczny w Łodzi
Postepy Hig Med Dosw (Online) 68:1452-63. 2014..Some archaeons, such as Haloferax mediterranei and Sulfolobus islandicus can synthesize antimicrobial agents which are called archaeocins...
- Transcriptional regulator-mediated activation of adaptation genes triggers CRISPR de novo spacer acquisitionTao Liu
State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, P R China
Nucleic Acids Res 43:1044-55. 2015..Here we examine the transcriptional regulation of the conserved spacer acquisition genes in Type I-A of Sulfolobus islandicus REY15A...
- Structural characterization of ether lipids from the archaeon Sulfolobus islandicus by high-resolution shotgun lipidomicsSara Munk Jensen
Department of Biology and Nordic Center for Earth Evolution, University of Southern Denmark, Odense, Denmark Department of Physics, Chemistry and Pharmacy, University of Southern Denmark, Odense, Denmark
J Mass Spectrom 50:476-87. 2015..shotgun lipidomics to characterize, for the first time, the lipid complement of the archaeon Sulfolobus islandicus. To support the identification of lipids in S...
- Efficient 5'-3' DNA end resection by HerA and NurA is essential for cell viability in the crenarchaeon Sulfolobus islandicusQihong Huang
State Key Laboratory of Microbial Technology, Shandong University, 27 Shanda Nan Rd, Jinan, 250100, P R China
BMC Mol Biol 16:2. 2015..Here we employed Sulfolobus islandicus, a hyperthermophilic archaeon, as a model to investigate the in vivo functions of the ATPase/helicase HerA,..
- Virology. A virus that infects a hyperthermophile encapsidates A-form DNAFrank DiMaio
Department of Biochemistry, University of Washington, Seattle, WA 98195, USA
Science 348:914-7. 2015..The nonenveloped, rod-shaped virus SIRV2 (Sulfolobus islandicus rod-shaped virus 2) infects the hyperthermophilic acidophile Sulfolobus islandicus, which lives at 80°C ..
- The Effects of Temperature and Growth Phase on the Lipidomes of Sulfolobus islandicus and Sulfolobus tokodaiiSara Munk Jensen
Department of Biology and Nordic Center for Earth Evolution, University of Southern Denmark, Odense 5230, Denmark
Life (Basel) 5:1539-66. 2015..used shotgun lipidomics to study this effect as well as the influence of growth phase on the lipidomes of Sulfolobus islandicus and Sulfolobus tokodaii for the first time...
- The identification of a novel Sulfolobus islandicus CAMP-like peptide points to archaeal microorganisms as cell factories for the production of antimicrobial moleculesEugenio Notomista
Dipartimento di Biologia, Universita degli Studi di Napoli Federico II, Complesso Universitario Monte S Angelo, Via Cinthia, 80126, Naples, Italy
Microb Cell Fact 14:126. 2015..Cationic antimicrobial peptides (CAMPs) are small positively charged peptides with an amphipathic structure, active against Gram-positive and Gram-negative bacteria, fungi, as well as protozoa...
- Harnessing Type I and Type III CRISPR-Cas systems for genome editingYingjun Li
State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, China
Nucleic Acids Res 44:e34. 2016..Here, a method was developed to repurpose both CRISPR-Cas systems for genetic manipulation in Sulfolobus islandicus, a thermophilic archaeon...
- Transcriptome changes in STSV2-infected Sulfolobus islandicus REY15A undergoing continuous CRISPR spacer acquisitionCarlos León-Sobrino
Archaea Centre, Department of Biology, University of Copenhagen, Copenhagen, DK 2200, Denmark
Mol Microbiol 99:719-28. 2016A transcriptome study was performed on Sulfolobus islandicus REY15A actively undergoing CRISPR spacer acquisition from the crenarchaeal monocaudavirus STSV2 in rich and basal media over a 6 day period...
- The CRISPR-associated Csx1 protein of Pyrococcus furiosus is an adenosine-specific endoribonucleaseNolan F Sheppard
Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia 30602, USA
RNA 22:216-24. 2016..associated component of the Cmr effector complex, but is necessary for DNA silencing by the Cmr system in Sulfolobus islandicus. To investigate the function of the Csx1 protein, we characterized the activity of recombinant Pyrococcus ..
- The apt/6-Methylpurine Counterselection System and Its Applications in Genetic Studies of the Hyperthermophilic Archaeon Sulfolobus islandicusChangyi Zhang
Department of Microbiology, University of Illinois at Urbana Champaign, Urbana, Illinois, USA Carl R Woese Institute for Genomic Biology, University of Illinois at Urbana Champaign, Urbana, Illinois, USA
Appl Environ Microbiol 82:3070-81. 2016b>Sulfolobus islandicus serves as a model for studying archaeal biology as well as linking novel biology to evolutionary ecology using functional population genomics...
- Diverse CRISPR-Cas responses and dramatic cellular DNA changes and cell death in pKEF9-conjugated Sulfolobus speciesGuannan Liu
Archaea Centre, Department of Biology, University of Copenhagen, DK 2200 Copenhagen N, Denmark
Nucleic Acids Res 44:4233-42. 2016..Here we examined the conjugation cycle of pKEF9 in the recipient strain Sulfolobus islandicus REY15A...
- Knockout and functional analysis of two DExD/H-box family helicase genes in Sulfolobus islandicus REY15AXueguo Song
State Key Laboratory of Microbial Technology, Shandong University, 27 Shanda Nan Rd, Jinan, 250100, China
Extremophiles 20:537-46. 2016..In this study, we attempted gene knockout of 8 putative DExD/H-box helicases in Sulfolobus islandicus REY15A and obtained two deletion mutants, SiRe_0681 and SiRe_1605...
- Abundant Lysine Methylation and N-Terminal Acetylation in Sulfolobus islandicus Revealed by Bottom-Up and Top-Down ProteomicsEgor A Vorontsov
From the Structural Mass Spectrometry and Proteomics Unit, Structural Biology and Chemistry Department, Institut Pasteur, 75015 Paris, France
Mol Cell Proteomics 15:3388-3404. 2016..The data have been deposited to the ProteomeXchange with identifiers PXD003074 and PXD004179...
- A type III-B CRISPR-Cas effector complex mediating massive target DNA destructionWenyuan Han
Archaea Centre, Department of Biology, University of Copenhagen, Ole Maaløes Vej 5, Copenhagen Biocenter, DK 2200 Copenhagen N, Denmark
Nucleic Acids Res . 2016..The Sulfolobus islandicus type III-B Cmr-α system targets invading nucleic acid at both RNA and DNA levels and DNA targeting relies ..
- Functional characterization of the origin of replication of pRN1 from Sulfolobus islandicus REN1H1Chijioke J Joshua
Department of Plant and Microbial Biology, University of California, Berkeley, Berkeley, California, United States of America Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, California, United States of America Joint BioEnergy Institute, Emeryville, California, United States of America
PLoS ONE 8:e84664. 2013Plasmid pRN1 from Sulfolobus islandicus REN1H1 is believed to replicate by a rolling circle mechanism but its origin and mechanism of replication are not well understood...
- Folding and association of an extremely stable dimeric protein from Sulfolobus islandicusMarkus Zeeb
Laboratorium für Biochemie III, Universitat Bayreuth, D 95440 Bayreuth, Germany
J Mol Biol 336:227-40. 2004ORF56 is a plasmid-encoded protein from Sulfolobus islandicus, which probably controls the copy number of the pRN1 plasmid by binding to its own promotor...
- Characterization of the Sulfolobus host-SSV2 virus interactionPatrizia Contursi
Department of Biological Chemistry, Institute of Molecular Biology and Physiology, University of Copenhagen, Copenhagen, Denmark
Extremophiles 10:615-27. 2006..In this paper we report the findings of a comparative study of SSV2 physiology in the natural host, Sulfolobus islandicus REY15/4, versus the foreign host, Sulfolobus solfataricus, and provide evidence of differently regulated ..
- First insights into the entry process of hyperthermophilic archaeal virusesEmmanuelle R J Quemin
Institut Pasteur, Unité Biologie Moléculaire du Gène chez les Extrêmophiles, Departement de Microbiologie, Paris, France
J Virol 87:13379-85. 2013..we present the first study focusing on the primary interactions between the archaeal rod-shaped virus Sulfolobus islandicus rod-shaped virus 2 (SIRV2) (family Rudiviridae) and its hyperthermoacidophilic host, S. islandicus...
- Knockouts of RecA-like proteins RadC1 and RadC2 have distinct responses to DNA damage agents in Sulfolobus islandicusPeng Juan Liang
State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, China
J Genet Genomics 40:533-42. 2013..In this study, we performed phylogenetic and genetic analysis of two RadC proteins from Sulfolobus islandicus. RadC is closer to the KaiC lineage of cyanobacteria and proteobacteria than to the lineage of the ..
- Characterisation of the novel restriction endonuclease SuiI from Sulfolobus islandicusStefan Söllner
Institute of Biochemistry, University of Bayreuth, Universitatstrasse 30, 95447 Bayreuth, Germany
Extremophiles 10:629-34. 2006A restriction endonuclease activity from Sulfolobus islandicus REN2H1 was purified by phosphocellulose and cation exchange chromatography...
- Exceptional thermal stability and organic solvent tolerance of an esterase expressed from a thermophilic hostYuxia Mei
State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, 430070, People s Republic of China
Appl Microbiol Biotechnol 93:1965-74. 2012A protein expression system recently developed for the thermophilic crenarchaeon Sulfolobus islandicus was employed to produce recombinant protein for EstA, a thermophilic esterase encoded in the same organism...
- Modulation of CRISPR locus transcription by the repeat-binding protein Cbp1 in SulfolobusLing Deng
Archaea Centre, Department of Biology, University of Copenhagen, Ole Maaløes Vej 5, 2200N Copenhagen K, Denmark
Nucleic Acids Res 40:2470-80. 2012..cbp1 gene deletion from Sulfolobus islandicus REY15A produced a strong reduction in pre-crRNA yields from CRISPR loci but did not inhibit the foreign ..
- Patterns of gene flow define species of thermophilic ArchaeaHinsby Cadillo-Quiroz
Department of Microbiology and Institute for Genomic Biology, University of Illinois, Urbana Champaign, Urbana, Illinois, United States of America
PLoS Biol 10:e1001265. 2012..we use high-throughput genome sequencing to identify ongoing speciation in the thermoacidophilic Archaeon Sulfolobus islandicus. Patterns of homologous gene flow among genomes of 12 strains from a single hot spring in Kamchatka, ..
- Genomic evidence of rapid, global-scale gene flow in a Sulfolobus speciesDominic Mao
Department of Biological Sciences, University of Cincinnati, Cincinnati, OH 45221, USA
ISME J 6:1613-6. 2012Local populations of Sulfolobus islandicus diverge genetically with geographical separation, and this has been attributed to restricted transfer of propagules imposed by the unfavorable spatial distribution of acidic geothermal habitat...
- A broadly applicable gene knockout system for the thermoacidophilic archaeon Sulfolobus islandicus based on simvastatin selectionChangyi Zhang
Department of Microbiology, University of Illinois at Urbana Champaign, Urbana, IL, USA
Microbiology 158:1513-22. 2012..Interesting patterns of natural variation among Sulfolobus islandicus strains have been identified through genome sequencing...
- A synthetic arabinose-inducible promoter confers high levels of recombinant protein expression in hyperthermophilic archaeon Sulfolobus islandicusNan Peng
State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, People s Republic of China
Appl Environ Microbiol 78:5630-7. 2012..Here we report a versatile vector that confers high levels of protein expression in Sulfolobus islandicus, a hyperthermophilic crenarchaeon...
- An archaeal immune system can detect multiple protospacer adjacent motifs (PAMs) to target invader DNASusan Fischer
Department of Biology II, Ulm University, 89069 Ulm, Germany
J Biol Chem 287:33351-63. 2012..their molecular details have so far only been examined in three archaeal species: Sulfolobus solfataricus, Sulfolobus islandicus, and Pyrococcus furiosus...
- Structural and enzymatic characterization of the lactonase SisLac from Sulfolobus islandicusJulien Hiblot
URMITE UMR CNRS IRD 6236, IFR48, Faculte de Medecine et de Pharmacie, Universite de la Mediterranee, Marseille, France
PLoS ONE 7:e47028. 2012A new member of the Phosphotriesterase-Like Lactonases (PLL) family from the hyperthermophilic archeon Sulfolobus islandicus (SisLac) has been characterized...
- Identification and characterization of a highly conserved crenarchaeal protein lysine methyltransferase with broad substrate specificityYindi Chu
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Chaoyang District, Beijing, China
J Bacteriol 194:6917-26. 2012..crenarchaeal protein lysine methyltransferase, designated aKMT, from the hyperthermophilic crenarchaeon Sulfolobus islandicus. The enzyme was capable of transferring methyl groups to selected lysine residues in a substrate protein ..
- Insights into a viral lytic pathway from an archaeal virus-host systemJamie C Snyder
Department of Plant Sciences and Plant Pathology, Montana State University, Bozeman, Montana, USA
J Virol 87:2186-92. 2013Archaeal host cells infected by Sulfolobus turreted icosahedral virus (STIV) and Sulfolobus islandicus rod-shaped virus 2 (SIRV2) produce unusual pyramid-like structures on the cell surface prior to virus-induced cell lysis...
- Genetic manipulation in Sulfolobus islandicus and functional analysis of DNA repair genesChangyi Zhang
State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, People s Republic of China
Biochem Soc Trans 41:405-10. 2013Recently, a novel gene-deletion method was developed for the crenarchaeal model Sulfolobus islandicus, which is a suitable tool for addressing gene essentiality in depth...
- Genomics and biology of Rudiviruses, a model for the study of virus-host interactions in ArchaeaDavid Prangishvili
Institut Pasteur, Department of Microbiology, 25 rue du Dr Roux, Paris 75015, France
Biochem Soc Trans 41:443-50. 2013..In addition, a revised genome annotation of SIRV2 (Sulfolobus islandicus rod-shaped virus 2) is presented...
- Sulfolobus islandicus: a model system for evolutionary genomicsChangyi Zhang
Department of Microbiology, University of Illinois at Urbana Champaign, 601 S Goodwin Ave, Urbana, IL 61801, USA
Biochem Soc Trans 41:458-62. 2013b>Sulfolobus islandicus has been developed as a model system for combining approaches of evolutionary and molecular biology in Archaea...
- Specificity and function of archaeal DNA replication initiator proteinsRachel Y Samson
Sir William Dunn School of Pathology, Oxford University, South Parks Road, Oxford OX1 3RE, UK
Cell Rep 3:485-96. 2013..We find that the three origins in the single chromosome of the archaeon Sulfolobus islandicus are specified by distinct initiation factors...
- Genomics and genetics of Sulfolobus islandicus LAL14/1, a model hyperthermophilic archaeonCarole Jaubert
Departement de Microbiologie, Unité Biologie Moléculaire du Gène chez les Extrêmophiles, Institut Pasteur, Paris, France
Open Biol 3:130010. 2013The 2 465 177 bp genome of Sulfolobus islandicus LAL14/1, host of the model rudivirus SIRV2, was sequenced. Exhaustive comparative genomic analysis of S. islandicus LAL14/1 and the nine other completely sequenced S...
- Massive activation of archaeal defense genes during viral infectionTessa E F Quax
Biologie Moléculaire du Gène chez les Extrêmophiles, Institut Pasteur, Paris, France or
J Virol 87:8419-28. 2013..The Sulfolobus islandicus rod-shaped virus 2 (SIRV2) is a model to study virus-host interactions in Archaea...
- Augmenting the genetic toolbox for Sulfolobus islandicus with a stringent positive selectable marker for agmatine prototrophyChangyi Zhang
Department of Microbiology, University of Illinois at Urbana Champaign, Urbana, Illinois, USA
Appl Environ Microbiol 79:5539-49. 2013..In particular, Sulfolobus islandicus provides a powerful opportunity to explore natural variation via experimental functional genomics...
- Complete nucleotide sequence of the Sulfolobus islandicus multicopy plasmid pRN1P J Keeling
Department of Biochemistry, Dalhousie University, Halifax, Nova Scotia, Canada
Plasmid 35:141-4. 1996The complete sequence of the 5350-bp plasmid pRN1 from the crenarchaeote Sulfolobus islandicus has been determined...
- Biochemical and phylogenetic characterization of the dUTPase from the archaeal virus SIRVD Prangishvili
Max Planck Institute for Biochemistry, Am Klopferspitz 18a, 82152 Martinsried, Federal Republic of Germany
J Biol Chem 273:6024-9. 1998The derived amino acid sequence from a 474-base pair open reading frame in the genome of the Sulfolobus islandicus rod-shaped virus SIRV shows striking similarity to bacterial dCTP deaminases and to dUTPases from eukaryotes, bacteria, ..
- Sulfolobus islandicus plasmids pRN1 and pRN2 share distant but common evolutionary ancestryP J Keeling
Department of Biology, Indiana University, Bloomington 47405, USA
Extremophiles 2:391-3. 1998The complete sequence of the plasmid pRN2 from the thermoacidophile Sulfolobus islandicus has been determined. The plasmid was found to be circular and 6959 bp in length. S...
- Molecular analysis of pDL10 from Acidianus ambivalens reveals a family of related plasmids from extremely thermophilic and acidophilic archaeaA Kletzin
Institute of Microbiology and Genetics, Darmstadt University of Technology, 64287 Darmstadt, Germany
Genetics 152:1307-14. 1999..The pDL10 Rep protein shared 45-80% identical residues with homologous protein genes encoded by the Sulfolobus islandicus plasmids pRN1 and pRN2...
- The genetic element pSSVx of the extremely thermophilic crenarchaeon Sulfolobus is a hybrid between a plasmid and a virusH P Arnold
Max Planck Institut fur Biochemie, 82152 Martinsried, Germany
Mol Microbiol 34:217-26. 1999A new Sulfolobus islandicus strain, REY15/4, harboured both a novel fusellovirus, SSV2, and a small plasmid, pSSVx. The plasmid spread in S...
- A novel lipothrixvirus, SIFV, of the extremely thermophilic crenarchaeon SulfolobusH P Arnold
Max Planck Institut fur Biochemie, Martinsried, 82152, Germany
Virology 267:252-66. 2000We describe a novel lipothrixvirus, SIFV, of the crenarchaeotal archaeon Sulfolobus islandicus. SIFV (S. islandicus filamentous virus) has a linear virion with a linear double-stranded DNA genome...
- Sulfolobicins, specific proteinaceous toxins produced by strains of the extremely thermophilic archaeal genus SulfolobusD Prangishvili
Max Plank Institute für Biochemie, 82152 Martinsried, Germany
J Bacteriol 182:2985-8. 2000Several novel strains of "Sulfolobus islandicus" produced proteinaceous toxins, termed sulfolobicins, which killed cells of other strains of the same species, as well as of Sulfolobus solfataricus P1 and Sulfolobus shibatae B12, but not ..
- Evolution of the family of pRN plasmids and their integrase-mediated insertion into the chromosome of the crenarchaeon Sulfolobus solfataricusX Peng
Microbial Genome Group Institute of Molecular Biology, University of Copenhagen, Solvgade 83H, Copenhagen, DK 1307, Denmark
J Mol Biol 303:449-54. 2000Plasmid pHEN7 from Sulfolobus islandicus was sequenced (7.83 kb) and shown to belong to the archaeal pRN family, which includes plasmids pRN1, pRN2, pSSVx and pDL10 that share a large conserved sequence region...
- pING family of conjugative plasmids from the extremely thermophilic archaeon Sulfolobus islandicus: insights into recombination and conjugation in CrenarchaeotaK M Stedman
Max Planck Institut fur Biochemie, D 82152 Martinsried, Germany
J Bacteriol 182:7014-20. 2000..The efficient occurrence of these recombination processes is further evidence for the striking plasticity of the Sulfolobus genome...
- Thermostable and site-specific DNA binding of the gene product ORF56 from the Sulfolobus islandicus plasmid pRN1, a putative archael plasmid copy control proteinG Lipps
University of Bayreuth, Biochemistry II, Universitatsstrasse 30, D 95447 Bayreuth, Germany
Nucleic Acids Res 29:904-13. 2001..we report on the product of the gene orf56 from plasmid pRN1 of the acidophilic and thermophilic archaeon Sulfolobus islandicus. orf56 has not been characterised yet but low sequence similarily to several eubacterial plasmid-encoded ..
- The protein ORF80 from the acidophilic and thermophilic archaeon Sulfolobus islandicus binds highly site-specifically to double-stranded DNA and represents a novel type of basic leucine zipper proteinG Lipps
University Bayreuth, Biochemistry II, Universitatsstrasse 30, 95447 Bayreuth, Germany
Nucleic Acids Res 29:4973-82. 2001The cryptic high copy number plasmid pRN1 from the thermophilic and acidophilic crenarchaeote Sulfolobus islandicus shares three conserved open reading frames with other S.islandicus plasmids...
- A novel type of replicative enzyme harbouring ATPase, primase and DNA polymerase activityGeorg Lipps
Department of Biochemistry, University of Bayreuth, Universitatsstrasse 30, Germany
EMBO J 22:2516-25. 2003..The protein ORF904, encoded by the plasmid pRN1 from the thermoacidophile archaeon Sulfolobus islandicus, is a highly compact multifunctional enzyme with ATPase, primase and DNA polymerase activity...
- Structure of a bifunctional DNA primase-polymeraseGeorg Lipps
Institute of Biochemistry, University of Bayreuth, Universitatsstrasse 30, D 95447 Bayreuth, Germany
Nat Struct Mol Biol 11:157-62. 2004..activities are combined, however, in newly identified replicases from archaeal plasmids, such as pRN1 from Sulfolobus islandicus. Here we present a structure-function analysis of the pRN1 primase-polymerase (prim-pol) domain...
- The replication protein of the Sulfolobus islandicus plasmid pRN1G Lipps
Biochemistry, University of Bayreuth, Universitatstrasse 30, 95447 Bayreuth, Germany
Biochem Soc Trans 32:240-4. 2004..This domain constitutes the first member of the DNA polymerase family E. Based on the biochemical characterization of the gene products a model of how pRN1 is replicated in vivo is proposed...
- Transcription of the rod-shaped viruses SIRV1 and SIRV2 of the hyperthermophilic archaeon sulfolobusAlexandra Kessler
Department of Microbiology, University of Regensburg, Regensburg, Germany
J Bacteriol 186:7745-53. 2004..analysis of gene expression of these viruses at different time points after infection of the host cell, Sulfolobus islandicus, and of the mapping of transcriptional start sites...
- Site-specific binding of the 9.5 kilodalton DNA-binding protein ORF80 visualized by atomic force microscopyM Lysetska
Lehrstuhl für Physikalische Chemie II and Lehrstuhl für Biochemie, Universitat Bayreuth, 95440 Bayreuth, Germany
Biomacromolecules 6:1252-7. 2005..The measurements allow an estimate of the stoichiometry of the DNA-protein complexes. In contrast to previous results, the single-molecule experiments suggest that only a low number of ORF80 molecules bind to a DNA-binding site...
- Recombination shapes the natural population structure of the hyperthermophilic archaeon Sulfolobus islandicusRachel J Whitaker
Department of Plant and Microbial Biology, University of California, Berkeley, USA
Mol Biol Evol 22:2354-61. 2005..We investigated the genetic structure of a thermoacidophilic crenarchaeal species, Sulfolobus islandicus, using multilocus sequence analysis of six variable protein-coding loci on a set of 60 isolates from the ..
- Two novel conjugative plasmids from a single strain of SulfolobusGael Erauso
Laboratory of Microbiology, Wageningen University, The Netherlands
Microbiology 152:1951-68. 2006Two conjugative plasmids (CPs) were isolated and characterized from the same 'Sulfolobus islandicus' strain, SOG2/4. The plasmids were separated from each other and transferred into Sulfolobus solfataricus...
- Crystallization and preliminary X-ray diffraction analysis of protein 14 from Sulfolobus islandicus filamentous virus (SIFV)Adeline Goulet
Architecture et Fonction des Macromolecules Biologiques, CNRS and Universités d Aix Marseille I et II, UMR 6098, Case 932, 163 Avenue de Luminy, 13288 Marseille Cedex 9, France
Acta Crystallogr Sect F Struct Biol Cryst Commun 62:884-6. 2006..1, c = 132.4 A. Diffraction data were collected to a maximum acceptable resolution of 2.95 and 3.20 A for the SeMet-labelled and native protein, respectively...
- A spreadable, non-integrative and high copy number shuttle vector for Sulfolobus solfataricus based on the genetic element pSSVx from Sulfolobus islandicusTiziana Aucelli
Istituto di Biochimica delle Proteine, Via P Castellino 111, 80131 CNR, Napoli, Italy
Nucleic Acids Res 34:e114. 2006The pSSVx genetic element from Sulfolobus islandicus REY15/4 is a hybrid between a plasmid and a fusellovirus, able to be maintained in non-integrative form and to spread when the helper SSV2 virus is present in the cells...
- An active nonautonomous mobile element in Sulfolobus islandicus REN1H1Silvia Berkner
Department of Biochemistry, University of Bayreuth, 95440 Bayreuth, Germany
J Bacteriol 189:2145-9. 2007In the crenarchaeote Sulfolobus islandicus REN1H1, a mobile element of 321 bp length has been shown to be active. It does not contain terminal inverted repeats and transposes by a replicative mechanism...
- Characterization of the transcriptional activity of the cryptic plasmid pRN1 from Sulfolobus islandicus REN1H1 and regulation of its replication operonSilvia Berkner
Department of Biochemistry, University of Bayreuth, 95440 Bayreuth, Germany
J Bacteriol 189:1711-21. 2007The plasmid pRN1 from Sulfolobus islandicus REN1H1 belongs to the crenarchaeal plasmid family pRN...
- Small multicopy, non-integrative shuttle vectors based on the plasmid pRN1 for Sulfolobus acidocaldarius and Sulfolobus solfataricus, model organisms of the (cren-)archaeaSilvia Berkner
Department of Biochemistry, University of Bayreuth, 95440 Bayreuth, Germany
Nucleic Acids Res 35:e88. 2007..of a series of Sulfolobus-Escherichia coli shuttle vectors based on the small multicopy plasmid pRN1 from Sulfolobus islandicus. Selection in suitable uracil auxotrophs is provided through inclusion of pyrEF genes in the plasmid...
- Transcriptional analysis of the genetic element pSSVx: differential and temporal regulation of gene expression reveals correlation between transcription and replicationPatrizia Contursi
Dipartimento di Biologia Strutturale e Funzionale, Universita degli Studi di Napoli Federico II, Complesso Universitario Monte S Angelo, Via Cinthia, Napoli, Italy
J Bacteriol 189:6339-50. 2007pSSVx from Sulfolobus islandicus strain REY15/4 is a hybrid between a plasmid and a fusellovirus...
- Structure of the acidianus filamentous virus 3 and comparative genomics of related archaeal lipothrixvirusesGisle Vestergaard
Centre for Comparative Genomics and Danish Archaea Centre, Department of Molecular Biology, Biocenter, Copenhagen University, Ole Maaløes Vej 5, DK 2200 Copenhagen N, Denmark
J Virol 82:371-81. 2008..over large regions, with this similarity extending partly to the earlier described betalipothrixvirus Sulfolobus islandicus filamentous virus...
- Evidence for the horizontal transfer of an integrase gene from a fusellovirus to a pRN-like plasmid within a single strain of Sulfolobus and the implications for plasmid survivalXu Peng
Danish Archaea Centre, Department of Molecular Biology, Biocenter, Copenhagen University, Ole Maaløes Vej 5, Copenhagen N, Denmark
Microbiology 154:383-91. 2008..This is believed to be the first report of a pRN plasmid sharing its natural host with a fusellovirus and carrying a highly similar integrase gene...
- Molecular biology of the pRN1 plasmid from Sulfolobus islandicusGeorg Lipps
University of Applied Sciences of Northwestern Switzerland FHNW, School of Life Sciences, Institute of Chemistry and Bioanalytics, Gründenstrasse 40, 4132 Muttenz, Switzerland
Biochem Soc Trans 37:42-5. 2009The pRN1 plasmid is a rather small multicopy plasmid which was isolated from a Sulfolobus islandicus strain in 1993 by Wolfram Zillig and co-workers...
- Genetic analyses in the hyperthermophilic archaeon Sulfolobus islandicusQunxin She
Danish Archaea Centre, Department of Biology, University of Copenhagen, Biocenter, Ole Maaloes Vej 5, DK 2200 Copenhagen N, Denmark
Biochem Soc Trans 37:92-6. 2009..In the last few years, we have focused on developing genetic systems for Sulfolobus islandicus using pyrEF as a selection marker and versatile genetic tools have been developed, including methods for ..
- Viral biogeography revealed by signatures in Sulfolobus islandicus genomesNicole L Held
Department of Microbiology, University of Illinois, Urbana Champaign, Urbana, IL 61801, USA
Environ Microbiol 11:457-66. 2009..The biogeographic distribution of viruses that infect Sulfolobus islandicus is investigated using genome comparisons among four newly identified, integrated, Sulfolobus spindle-..
- Synergy of NMR, computation, and X-ray crystallography for structural biologyBlair R Szymczyna
Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA, 92037, USA
Structure 17:499-507. 2009..overall strategy for rapidly determining the structure of the coat protein C-terminal domain from the Sulfolobus islandicus rod-shaped virus (SIRV)...
- Biogeography of the Sulfolobus islandicus pan-genomeMichael L Reno
Department of Microbiology and Institute for Genomic Biology, University of Illinois at Urbana Champaign, Urbana, IL 61801, USA
Proc Natl Acad Sci U S A 106:8605-10. 2009..Through population-scale comparative genomics of 7 Sulfolobus islandicus genomes from 3 locations, we demonstrate the biogeographical structure of the pan-genome of this species, ..
- Unmarked gene deletion and host-vector system for the hyperthermophilic crenarchaeon Sulfolobus islandicusLing Deng
Huazhong Agricultural University, Wuhan, People s Republic of China
Extremophiles 13:735-46. 2009b>Sulfolobus islandicus is being used as a model for studying archaeal biology, geo-biology and evolution. However, no genetic system is available for this organism. To produce an S...
- Structure, function, and targets of the transcriptional regulator SvtR from the hyperthermophilic archaeal virus SIRV1Florence Guillière
Institut Pasteur, Unité de RMN des Biomolécules, CNRS URA 2185, 75015 Paris, France
J Biol Chem 284:22222-37. 2009..SvtR (formerly called gp08) from the rod-shaped virus SIRV1, which infects the hyperthermophilic archaeon Sulfolobus islandicus that thrives at 85 degrees C in hot acidic springs. The protein forms a dimer in solution...
- A unique virus release mechanism in the ArchaeaAriane Bize
Biologie Moléculaire du Gène chez les Extrêmophiles, Institut Pasteur, 25 rue du Docteur Roux, 75724 Paris Cedex 15, France
Proc Natl Acad Sci U S A 106:11306-11. 2009..Here, we demonstrate that the virions of the archaeal Sulfolobus islandicus rod-shaped virus 2 (SIRV2) are released from the host cell through a mechanism, involving the formation of ..
- Exploitation of plant and archaeal viruses in bionanotechnologyDavid J Evans
Department of Biological Chemistry, John Innes Centre, Norwich Research Park, Norwich NR4 7UH, UK
Biochem Soc Trans 37:665-70. 2009..SIRV2 (Sulfolobus islandicus rod-shaped virus 2), is a robust archaeal virus, resistant to high temperature and low pH...
- Structure-based stability analysis of an extremely stable dimeric DNA binding protein from Sulfolobus islandicusUlrich Weininger
Institut fur Physik, Biophysik, Martin Luther Universitat Halle Wittenberg, D 06120 Halle Saale, Germany
Biochemistry 48:10030-7. 2009ORF56 is a small and thermodynamically extremely stable dimeric protein from the archaeon Sulfolobus islandicus. This DNA binding protein is encoded on plasmid pRN1 and possibly controls the copy number of the plasmid...
- An upstream activation element exerting differential transcriptional activation on an archaeal promoterNan Peng
State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China
Mol Microbiol 74:928-39. 2009..of the Sulfolobus solfataricus araS gene encoding an arabinose binding protein was characterized using an Sulfolobus islandicus reporter gene system...
- Acidianus filamentous virus 1 coat proteins display a helical fold spanning the filamentous archaeal viruses lineageAdeline Goulet
Architecture et Fonction des Macromolecules Biologiques, Centre national de la Recherche Scientifique and Universités Aix Marseille I and II, Architecture et Fonction des Macromolecules Biologiques, Unite Mixte de Recherche 6098, Case 932, 163 Avenue de Luminy, 13288 Marseille Cedex 9, France
Proc Natl Acad Sci U S A 106:21155-60. 2009..The four-helix bundle fold of the MCPs from AFV1 is identical to that of the coat protein (CP) of Sulfolobus islandicus rod-shaped virus (SIRV), a member of the Rudiviridae family...
- The Sulfolobus rod-shaped virus 2 encodes a prominent structural component of the unique virion release system in ArchaeaTessa E F Quax
Institut Pasteur, Molecular Biology of the Gene in Extremophiles Unit, 75724 Paris Cedex 15, France
Virology 404:1-4. 2010..Here we present results of a protein analysis of Sulfolobus islandicus cells infected with the rudivirus SIRV2, which enable us to postulate SIRV2-encoded protein P98 as the ..
- Revealing the essentiality of multiple archaeal pcna genes using a mutant propagation assay based on an improved knockout methodChangyi Zhang
State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, PR China
Microbiology 156:3386-97. 2010..To study the mechanism of archaeal sliding clamps, we sought to generate knockouts for each pcna gene in Sulfolobus islandicus, a hyperthermophilic crenarchaeon, but failed with two conventional knockout methods...
- Transcription termination in the plasmid/virus hybrid pSSVx from Sulfolobus islandicusPatrizia Contursi
Archaeal Genetics Laboratory, Department of Molecular Biology, University of Copenhagen, Copenhagen Biocenter Ole Maaløes Vej 5, 2200, Copenhagen N, Denmark
Extremophiles 14:453-63. 2010The pSSVx from Sulfolobus islandicus, strain REY15/4, is a hybrid between a plasmid and a fusellovirus...
- Evolutionary rates and gene dispensability associate with replication timing in the archaeon Sulfolobus islandicusKenneth M Flynn
Department of Molecular, Cellular, and Biomedical Sciences, University of New Hampshire, USA
Genome Biol Evol 2:859-69. 2010..Therefore, in this genus of Archaea as well as in many Bacteria, evolutionary rates and variation in genome content associate with replication timing...
- Dynamic properties of the Sulfolobus CRISPR/Cas and CRISPR/Cmr systems when challenged with vector-borne viral and plasmid genes and protospacersSoley Gudbergsdottir
Archaea Centre, Department of Biology, University of Copenhagen, Ole Maaløes Vej 5, 2200N Copenhagen, Denmark
Mol Microbiol 79:35-49. 2011..pyrE/pyrF genes and transformed into uracil auxotrophic hosts derived from Sulfolobus solfataricus P2 or Sulfolobus islandicus REY15A...
- C68 from the Sulfolobus islandicus plasmid-virus pSSVx is a novel member of the AbrB-like transcription factor familyPatrizia Contursi
Dipartimento di Biologia Strutturale e Funzionale, Universita degli Studi di Napoli Federico II, Complesso Universitario Monte S Angelo, Via Cinthia, Naples, Italy
Biochem J 435:157-66. 2011The genetic element pSSVx from Sulfolobus islandicus, strain REY15/4, is a hybrid between a plasmid and a fusellovirus. This plasmid-virus hybrid infects several species of the hyperthermophilic acidophilic crenarchaeon Sulfolobus...
- Archaeal promoter architecture and mechanism of gene activationNan Peng
State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, 430070 Wuhan, Hubei, China
Biochem Soc Trans 39:99-103. 2011Sulfolobus solfataricus and Sulfolobus islandicus contain several genes exhibiting D-arabinose-inducible expression and these systems are ideal for studying mechanisms of archaeal gene expression...
- Genome analyses of Icelandic strains of Sulfolobus islandicus, model organisms for genetic and virus-host interaction studiesLi Guo
Archaea Centre, Department of Biology, Copenhagen University, Ole Maaløes Vej 5, DK 2200N Copenhagen, Denmark
J Bacteriol 193:1672-80. 2011The genomes of two Sulfolobus islandicus strains obtained from Icelandic solfataras were sequenced and analyzed. Strain REY15A is a host for a versatile genetic toolbox...
- Simple and elegant design of a virion egress structure in ArchaeaTessa E F Quax
Institut Pasteur, Biologie Moléculaire du Gène chez les Extrêmophiles, 75015 Paris, France
Proc Natl Acad Sci U S A 108:3354-9. 2011..Here we describe in detail the structure and composition of VAPs formed by the Sulfolobus islandicus rod-shaped virus 2 (SIRV2) in cells of its hyperthermophilic archaeal host...
- Crystallization and preliminary X-ray diffraction analysis of the hyperthermophilic Sulfolobus islandicus lactonaseGuillaume Gotthard
Architecture et Fonction des Macromolecules Biologiques, CNRS Aix Marseille Universite, 13288 Marseille, France
Acta Crystallogr Sect F Struct Biol Cryst Commun 67:354-7. 2011..of the PLL family has been identified (SisPox) and its gene was cloned from the hyperthermophilic archeon Sulfolobus islandicus. Owing to its hyperthermostable architecture, SisPox appears to be a good candidate for engineering ..
- Exceptional virion release mechanism: one more surprise from archaeal virusesDavid Prangishvili
Institut Pasteur, Department of Microbiology, Biologie Moléculaire du Gène chez les Extrêmophiles, 75015 Paris, France
Curr Opin Microbiol 14:315-20. 2011..Here we summarize available data on the extraordinary egress mechanism exploited by the Sulfolobus islandicus rod-shaped virus SIRV2 and the Sulfolobus turreted icosahedral virus STIV...
- Characterization of Sulfolobus islandicus rod-shaped virus 2 gp19, a single-strand specific endonucleaseAndrew F Gardner
New England Biolabs, Inc, Ipswich, MA 01938, USA
Extremophiles 15:619-24. 2011The hyperthermophilic Sulfolobus islandicus rod-shaped virus 2 (SIRV2) encodes a 25-kDa protein (SIRV2gp19) annotated as a hypothetical protein with sequence homology to the RecB nuclease superfamily...
- Deletion of the topoisomerase III gene in the hyperthermophilic archaeon Sulfolobus islandicus results in slow growth and defects in cell cycle controlXiyang Li
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China
J Genet Genomics 38:253-9. 2011..in vivo role of archaeal topo III, we constructed and characterized a topo III gene deletion mutant of Sulfolobus islandicus. The mutant was viable but grew more slowly than the wild-type strain, especially in a nutrient-poor ..
- Biochemical characterization of a structure-specific resolving enzyme from Sulfolobus islandicus rod-shaped virus 2Andrew F Gardner
New England Biolabs, Inc, Ipswich, Massachusetts, United States of America
PLoS ONE 6:e23668. 2011b>Sulfolobus islandicus rod shaped virus 2 (SIRV2) infects the archaeon Sulfolobus islandicus at extreme temperature (70°C-80°C) and acidity (pH 3)...
- Development of a simvastatin selection marker for a hyperthermophilic acidophile, Sulfolobus islandicusTao Zheng
State Key Laboratory of Microbial Technology, Shandong University, Jinan, People s Republic of China
Appl Environ Microbiol 78:568-74. 2012We report here a novel selectable marker for the hyperthermophilic crenarchaeon Sulfolobus islandicus. The marker cassette is composed of the sac7d promoter and the hmg gene coding for the 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) ..
- An expansion of age constraints for microbial clades that lack a conventional fossil record using phylogenomic datingCarrine E Blank
Department of Geosciences, University of Montana, 32 Campus Drive 1296, Missoula, MT 59812 1296, USA
J Mol Evol 73:188-208. 2011..Similar analyses also predicted that the ancestor to the Sulfolobus solfataricus-Sulfolobus islandicus clade was able to metabolize phenol using catechol dioxygenase, so this clade was assigned a maximum age ..
- Genomic comparison of archaeal conjugative plasmids from SulfolobusBo Greve
Danish Archaea Centre, Institute of Molecular Biology, Copenhagen University, Sølvgade 83H, DK 1307 Copenhagen K, Denmark
Archaea 1:231-9. 2004....
- Biochemical and structural characterization of self-assembling viral particlesREBECCA ETHEL TAUROG; Fiscal Year: 2010The Sulfolobus islandicus rod-shaped virus (SIRV) is a fibrous virus that infects thermophilic, acidophilic Archaea found in thermal vents and hot springs around the world...
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- Archaeal plasmid vector systemPatent Number: EP1990417-A1; Date:2008-11-12
- Archaeal plasmid vector systemPatent Number: WO2008138579-A1; Date:2008-11-20
- AN ENZYME FOR THE PREPARATION OF METHYLMALONATE SEMIALDEHYDEPatent Number: WO2011157573-A2; Date:2011-12-22