Trypanosoma brucei TREU927

Summary

Alias: Trypanosoma brucei strain TREU927

Top Publications

  1. ncbi tbCPSF30 depletion by RNA interference disrupts polycistronic RNA processing in Trypanosoma brucei
    Edward F Hendriks
    School of Biological Sciences, Division of Biochemistry, 2 205 Stopford Building, University of Manchester, Oxford Road, Manchester M13 9PT, United Kingdom
    J Biol Chem 278:26870-8. 2003
  2. pmc Chlamydomonas kinesin-II-dependent intraflagellar transport (IFT): IFT particles contain proteins required for ciliary assembly in Caenorhabditis elegans sensory neurons
    D G Cole
    Molecular, Cellular and Developmental Biology, Yale University, New Haven, Connecticut 06520 8103, USA
    J Cell Biol 141:993-1008. 1998
  3. pmc Galactose metabolism is essential for the African sleeping sickness parasite Trypanosoma brucei
    Janine R Roper
    Division of Biological Chemistry and Molecular Microbiology, The Wellcome Trust Biocentre, School of Life Sciences, University of Dundee, DD1 5EH Dundee, Scotland, United Kingdom
    Proc Natl Acad Sci U S A 99:5884-9. 2002
  4. ncbi Cloning of Trypanosoma brucei and Leishmania major genes encoding the GlcNAc-phosphatidylinositol de-N-acetylase of glycosylphosphatidylinositol biosynthesis that is essential to the African sleeping sickness parasite
    Tunhan Chang
    Division of Biological Chemistry and Molecular Microbiology, The Wellcome Trust Biocentre, The School of Life Sciences, University of Dundee, Dundee DD1 5EH, Scotland, United Kingdom
    J Biol Chem 277:50176-82. 2002
  5. pmc Novel roles for the flagellum in cell morphogenesis and cytokinesis of trypanosomes
    Linda Kohl
    INSERM U565 and CNRS UMR8646, Laboratoire de Biophysique, Museum national d histoire naturelle, 43 rue Cuvier, 75231 Paris Cedex 05, France
    EMBO J 22:5336-46. 2003
  6. pmc Genomic rearrangements and transcriptional analysis of the spliced leader-associated retrotransposon in RNA interference-deficient Trypanosoma brucei
    Kristin L Patrick
    Department of Epidemiology and Public Health, Yale University Medical School, 295 Congress Avenue, New Haven, CT 06536 0812, USA
    Mol Microbiol 67:435-47. 2008
  7. ncbi Trypanosoma brucei dihydrofolate reductase-thymidylate synthase: gene isolation and expression and characterization of the enzyme
    F Gamarro
    Department of Biochemistry and Biophysics, University of California, San Francisco 94143, USA
    Mol Biochem Parasitol 72:11-22. 1995
  8. ncbi Characterization of the Trypanosoma brucei homologue of a Trypanosoma cruzi flagellum-adhesion glycoprotein
    T Nozaki
    Laboratory of Molecular Parasitology, Rockefeller University, New York, NY 10021 6399, USA
    Mol Biochem Parasitol 82:245-55. 1996
  9. ncbi Conservation of genetic linkage between heat shock protein 100 and glycosylphosphatidylinositol-specific phospholipase C in Trypanosoma brucei and Trypanosoma cruzi
    M B Redpath
    Department of Biochemistry, University of Cambridge, UK
    Mol Biochem Parasitol 94:113-21. 1998
  10. ncbi Knockout of the glutamate dehydrogenase gene in bloodstream Trypanosoma brucei in culture has no effect on editing of mitochondrial mRNAs
    A M Estevez
    Howard Hughes Medical Institute, UCLA School of Medicine, Los Angeles, CA 90095 1662, USA
    Mol Biochem Parasitol 100:5-17. 1999

Scientific Experts

Detail Information

Publications125 found, 100 shown here

  1. ncbi tbCPSF30 depletion by RNA interference disrupts polycistronic RNA processing in Trypanosoma brucei
    Edward F Hendriks
    School of Biological Sciences, Division of Biochemistry, 2 205 Stopford Building, University of Manchester, Oxford Road, Manchester M13 9PT, United Kingdom
    J Biol Chem 278:26870-8. 2003
    ..This study is the first to identify and characterize a core component of the T. brucei CPSF and show its involvement in polycistronic RNA processing...
  2. pmc Chlamydomonas kinesin-II-dependent intraflagellar transport (IFT): IFT particles contain proteins required for ciliary assembly in Caenorhabditis elegans sensory neurons
    D G Cole
    Molecular, Cellular and Developmental Biology, Yale University, New Haven, Connecticut 06520 8103, USA
    J Cell Biol 141:993-1008. 1998
    ....
  3. pmc Galactose metabolism is essential for the African sleeping sickness parasite Trypanosoma brucei
    Janine R Roper
    Division of Biological Chemistry and Molecular Microbiology, The Wellcome Trust Biocentre, School of Life Sciences, University of Dundee, DD1 5EH Dundee, Scotland, United Kingdom
    Proc Natl Acad Sci U S A 99:5884-9. 2002
    ..The results show that enzymes and transporters involved in galactose metabolism may be considered as potential therapeutic targets against African trypanosomiasis...
  4. ncbi Cloning of Trypanosoma brucei and Leishmania major genes encoding the GlcNAc-phosphatidylinositol de-N-acetylase of glycosylphosphatidylinositol biosynthesis that is essential to the African sleeping sickness parasite
    Tunhan Chang
    Division of Biological Chemistry and Molecular Microbiology, The Wellcome Trust Biocentre, The School of Life Sciences, University of Dundee, Dundee DD1 5EH, Scotland, United Kingdom
    J Biol Chem 277:50176-82. 2002
    ..These results suggest that the stabilities of other glycosylphosphatidylinositol pathway enzymes are not dependent on GlcNAc-PI de-N-acetylase levels...
  5. pmc Novel roles for the flagellum in cell morphogenesis and cytokinesis of trypanosomes
    Linda Kohl
    INSERM U565 and CNRS UMR8646, Laboratoire de Biophysique, Museum national d histoire naturelle, 43 rue Cuvier, 75231 Paris Cedex 05, France
    EMBO J 22:5336-46. 2003
    ..We show that flagellum elongation controls formation of cytoskeletal structures (present in the cell body) that act as molecular organizers of the cell...
  6. pmc Genomic rearrangements and transcriptional analysis of the spliced leader-associated retrotransposon in RNA interference-deficient Trypanosoma brucei
    Kristin L Patrick
    Department of Epidemiology and Public Health, Yale University Medical School, 295 Congress Avenue, New Haven, CT 06536 0812, USA
    Mol Microbiol 67:435-47. 2008
    ..We speculate that the small SLACS transcripts serve as substrates for the production of siRNAs to regulate SLACS expression...
  7. ncbi Trypanosoma brucei dihydrofolate reductase-thymidylate synthase: gene isolation and expression and characterization of the enzyme
    F Gamarro
    Department of Biochemistry and Biophysics, University of California, San Francisco 94143, USA
    Mol Biochem Parasitol 72:11-22. 1995
    ..The T. brucei DHFR has Ki values for antimicrobial antifolates pyrimethamine and trimethoprim which are significantly lower than the closely related T. cruzi or L. major DHFRs or than human DHFR...
  8. ncbi Characterization of the Trypanosoma brucei homologue of a Trypanosoma cruzi flagellum-adhesion glycoprotein
    T Nozaki
    Laboratory of Molecular Parasitology, Rockefeller University, New York, NY 10021 6399, USA
    Mol Biochem Parasitol 82:245-55. 1996
    ..Fla1 from both life-cycle stages is N-glycosylated. Fla1 from bloodstream-form T. brucei contains additional glycans, which can be liberated by treatment with mild acid, suggestive of phosphodiester linkages...
  9. ncbi Conservation of genetic linkage between heat shock protein 100 and glycosylphosphatidylinositol-specific phospholipase C in Trypanosoma brucei and Trypanosoma cruzi
    M B Redpath
    Department of Biochemistry, University of Cambridge, UK
    Mol Biochem Parasitol 94:113-21. 1998
    ..Thus, the hsp100 and gpi-plc genes are adjacent in T. brucei and this linkage is conserved in T. cruzi. This observation has been used to facilitate the isolation of a clone encoding a T. cruzi phospholipase C gene...
  10. ncbi Knockout of the glutamate dehydrogenase gene in bloodstream Trypanosoma brucei in culture has no effect on editing of mitochondrial mRNAs
    A M Estevez
    Howard Hughes Medical Institute, UCLA School of Medicine, Los Angeles, CA 90095 1662, USA
    Mol Biochem Parasitol 100:5-17. 1999
    ....
  11. ncbi Calmodulin-binding properties of the paraflagellar rod complex from Trypanosoma brucei
    E Ridgley
    Department of Biological Sciences, Southern Methodist University, Dallas, TX 75275, USA
    Mol Biochem Parasitol 109:195-201. 2000
  12. ncbi The coatomer of Trypanosoma brucei
    A G Maier
    Zentrum für Molekulare Biologie ZMBH, Im Nevenheimer Feld 282, 69120, Heidelberg, Germany
    Mol Biochem Parasitol 115:55-61. 2001
    ..The trypanosome coatomer complex was partially purified using a procedure similar to that used for bovine coatomer...
  13. pmc Rab5 and Rab11 mediate transferrin and anti-variant surface glycoprotein antibody recycling in Trypanosoma brucei
    Arun Pal
    Wellcome Trust Laboratories for Molecular Parasitology, Department of Biological Sciences and Centre for Molecular Microbiology and Infection, Imperial College London, London, SW7 2AY, UK
    Biochem J 374:443-51. 2003
    ..The presence of an efficient mechanism for the removal of IgG bound to the external surface of T. brucei and its subsequent proteolysis within the recycling system suggests a role for this pathway in immune evasion...
  14. ncbi Trypanosoma brucei has two distinct mitochondrial DNA polymerase beta enzymes
    Tina T Saxowsky
    Department of Biological Chemistry, Johns Hopkins School of Medicine, Baltimore, Maryland 21205, USA
    J Biol Chem 278:49095-101. 2003
    ....
  15. ncbi Downregulation of the nuclear-encoded subunits of the complexes III and IV disrupts their respective complexes but not complex I in procyclic Trypanosoma brucei
    Anton Horváth
    Faculty of Natural Sciences, Comenius University, Bratislava, Slovakia
    Mol Microbiol 58:116-30. 2005
    ..The apparent absence in T. brucei procyclics of a supercomplex composed of complexes I and III may represent an ancestral state of the respiratory chain...
  16. ncbi In vitro and in vivo analysis of the major type I protein arginine methyltransferase from Trypanosoma brucei
    Michel Pelletier
    Department of Microbiology and Immunology, Witebsky Center for Microbial Pathogenesis and Immunology, SUNY Buffalo School of Medicine, Buffalo, NY 14214, USA
    Mol Biochem Parasitol 144:206-17. 2005
    ..brucei. The strong conservation of PRMT1 homologs between protozoa and humans highlights the importance of arginine methylation as a regulatory mechanism in eukaryotes...
  17. ncbi The myo-inositol-1-phosphate synthase gene is essential in Trypanosoma brucei
    K L Martin
    Division of Biological Chemistry and Molecular Microbiology, School of Life Sciences, University of Dundee, Dundee DD1 5EH, Scotland, UK
    Biochem Soc Trans 33:983-5. 2005
    ..brucei and that the de novo synthesized myo-inositol is used for the formation of PI and GPI anchors...
  18. pmc The glycosylphosphatidylinositol (GPI) biosynthetic pathway of bloodstream-form Trypanosoma brucei is dependent on the de novo synthesis of inositol
    Kirstee L Martin
    Division of Biological Chemistry and Molecular Microbiology, The School of Life Sciences, University of Dundee, Dundee DD1 5EH, Scotland, UK
    Mol Microbiol 61:89-105. 2006
    ..These results suggest that there is a distinction between de novo synthesized myo-inositol and that from the extracellular environment...
  19. pmc Roles of a Trypanosoma brucei 5'->3' exoribonuclease homolog in mRNA degradation
    Chi Ho Li
    Zentrum für Molekulare Biologie der Universität Heidelberg ZMBH, D 69120 Heidelberg, Germany
    RNA 12:2171-86. 2006
    ..We conclude that in trypanosomes 5'-->3' exonuclease activity is important in degradation of highly unstable, regulated mRNAs, but that for other mRNAs another step is more important in determining the decay rate...
  20. pmc Spliced leader RNA gene transcription in Trypanosoma brucei requires transcription factor TFIIH
    Ju Huck Lee
    Department of Genetics and Developmental Biology, University of Connecticut Health Center, 263 Farmington Avenue, Farmington, CT 06030 3301, USA
    Eukaryot Cell 6:641-9. 2007
    ..Since we also identified orthologues of the TFIIH subunits XPB, p52/TFB2, and p44/SSL1 copurifying with TbXPD, we concluded that the parasite harbors a TFIIH which is indispensable for SL RNA gene transcription...
  21. pmc Trypanosoma brucei RNA binding proteins p34 and p37 mediate NOPP44/46 cellular localization via the exportin 1 nuclear export pathway
    Kristina Hellman
    Department of Microbiology and Immunology and The Witebsky Center for Microbial Pathogenesis and Immunology, 253 Biomedical Research Building, University at Buffalo, Buffalo, New York 14214, USA
    Eukaryot Cell 6:2206-13. 2007
    ..Together, our results demonstrate that p34 and p37 regulate NOPP44/46 cellular localization by facilitating their association with exportin 1...
  22. ncbi A repetitive protein essential for the flagellum attachment zone filament structure and function in Trypanosoma brucei
    Sue Vaughan
    Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK
    Protist 159:127-36. 2008
    ..The cytokinesis defects provide further evidence for the role of an attached flagellum in cellular morphogenesis in these trypanosomes...
  23. pmc Mitochondrial origin-binding protein UMSBP mediates DNA replication and segregation in trypanosomes
    Neta Milman
    Department of Parasitology, The Kuvin Center for the Study of Infectious and Tropical Diseases, Hebrew University Hadassah Medical School, Jerusalem 91120, Israel
    Proc Natl Acad Sci U S A 104:19250-5. 2007
    ....
  24. pmc Identification of a novel chromosomal passenger complex and its unique localization during cytokinesis in Trypanosoma brucei
    Ziyin Li
    Department of Pharmaceutical Chemistry, University of California San Francisco, San Francisco, California, United States of America
    PLoS ONE 3:e2354. 2008
    ....
  25. doi Monothiol glutaredoxin-1 is an essential iron-sulfur protein in the mitochondrion of African trypanosomes
    Marcelo A Comini
    Centre of Biochemistry, Heidelberg University, Im Neuenheimer Feld 504, D 69120 Heidelberg, Germany
    J Biol Chem 283:27785-98. 2008
    ..The results point to an essential role of the mitochondrial 1-C-Grx1 in the iron metabolism of these parasites...
  26. pmc The F(0)F(1)-ATP synthase complex contains novel subunits and is essential for procyclic Trypanosoma brucei
    Alena Zíková
    Seattle Biomedical Research Institute, Seattle, WA, USA
    PLoS Pathog 5:e1000436. 2009
    ..Hence, the two novel proteins appear essential for the structural organization of the functional complex and regulation of mitochondrial energy generation in these organisms is more complicated than previously thought...
  27. pmc Trypanosoma brucei pteridine reductase 1 is essential for survival in vitro and for virulence in mice
    Natasha Sienkiewicz
    Division of Biological Chemistry and Drug Discovery, College of Life Sciences, University of Dundee, Dundee, UK
    Mol Microbiol 77:658-71. 2010
    ..RNAi cell lines were markedly less virulent than wild-type parasites in mice and virulence was restored in the (oe)RNAi line. Thus, PTR1 may be a drug target for human African trypanosomiasis...
  28. pmc The FACT subunit TbSpt16 is involved in cell cycle specific control of VSG expression sites in Trypanosoma brucei
    Viola Denninger
    Division of Cell and Molecular Biology, Sir Alexander Fleming Building, Imperial College, South Kensington, London SW72AZ, UK
    Mol Microbiol 78:459-74. 2010
    ..The chromatin remodeler FACT is therefore implicated in maintenance of repressed chromatin present at silent VSG ES promoters, but is also essential for chromosome segregation presumably through maintenance of functional centromeres...
  29. pmc Structure-function relationship of the Polo-like kinase in Trypanosoma brucei
    Zhonglian Yu
    Department of Microbiology and Molecular Genetics, University of Texas Medical School at Houston, 6431 Fannin Street, Houston, TX 77030, USA
    J Cell Sci 125:1519-30. 2012
    ..Together, these results suggest an essential role of T-loop phosphorylation in TbPLK activation and crucial roles of the PBD in regulating TbPLK activity and localization...
  30. doi Expression of the RNA recognition motif protein RBP10 promotes a bloodstream-form transcript pattern in Trypanosoma brucei
    Martin Wurst
    Zentrum fur Molekulare Biologie der Universitat Heidelberg, DKFZ ZMBH Alliance, Im Neuenheimer Feld 282, Heidelberg, Germany
    Mol Microbiol 83:1048-63. 2012
    ..Tethering of RBP10 to a reporter mRNA inhibited translation, and halved the abundance of the bound mRNA. We suggest that RBP10 may prevent the expression of regulatory proteins that are specific to the procyclic form...
  31. doi An interplay between Centrin2 and Centrin4 on the bi-lobed structure in Trypanosoma brucei
    Min Wang
    Department of Biological Sciences, National University of Singapore, Singapore
    Mol Microbiol 83:1153-61. 2012
    ..These results thus suggest a co-ordinated action between these two centrin proteins, where the cell cycle-dependent TbCentrin4 expression could regulate the abundance of TbCentrin2 on the bi-lobed structure...
  32. ncbi Metabolism and functions of trypanothione in the Kinetoplastida
    A H Fairlamb
    Department of Medical Parasitology, London School of Hygiene and Tropical Medicine, United Kingdom
    Annu Rev Microbiol 46:695-729. 1992
    ....
  33. ncbi Molecular characterization of two invariant surface glycoproteins specific for the bloodstream stage of Trypanosoma brucei
    K Ziegelbauer
    Max Planck Institut fur Biologie, Abteilung Membranbiochemie, Tubingen, Federal Republic of Germany
    J Biol Chem 267:10797-803. 1992
    ..They can be detected in all T. brucei brucei variant clones investigated. Both polypeptides are distributed over the entire surface of the parasite...
  34. ncbi Purification and characterization of the membrane-form variant surface glycoprotein hydrolase of Trypanosoma brucei
    R Bülow
    J Biol Chem 261:11918-23. 1986
    ..Reconstitution experiments into phospholipid vesicles show that the enzyme can hydrolyze mfVSG when present in the same phospholipid bilayer but not when present in separate bilayers...
  35. ncbi A major tyrosine-phosphorylated protein of Trypanosoma brucei is a nucleolar RNA-binding protein
    A Das
    Seattle Biomedical Research Institute, Seattle, Washington 98109, USA
    J Biol Chem 271:15675-81. 1996
    ..These studies suggest that Nopp44/46 may play a role in RNA metabolism in trypanosomes and raise the possibility that tyrosine phosphorylation may regulate the process...
  36. ncbi Characterization of a SR protein from Trypanosoma brucei with homology to RNA-binding cis-splicing proteins
    N Ismaili
    Department of Molecular Biology, Free University of Brussels, Rhode St Genese, Belgium
    Mol Biochem Parasitol 102:103-15. 1999
    ..These and other observations suggest that TSR1 may be involved in trans-splicing in T. brucei...
  37. pmc Critical roles of glycosylphosphatidylinositol for Trypanosoma brucei
    K Nagamune
    Department of Immunoregulation, Research Institute for Microbial Diseases, Osaka University, 3 1 Yamada oka, Suita, Osaka 565 0871, Japan
    Proc Natl Acad Sci U S A 97:10336-41. 2000
    ..Infectivity to tsetse flies is partially impaired, particularly in the early stage. Therefore, parasitespecific inhibition of GPI biosynthesis should be an effective chemotherapy target against African trypanosomiasis...
  38. ncbi The role of phosphomannose isomerase in Leishmania mexicana glycoconjugate synthesis and virulence
    A Garami
    Max Planck Institut fur Biologie, Corrensstrasse 38, Tubingen 72076, Federal Republic of Germany
    J Biol Chem 276:6566-75. 2001
    ..L. mexicana Delta lmexpmi provides the first conditional mannose-controlled system for parasite glycoconjugate assembly with potential applications for the investigation of their biosynthesis, intracellular sorting, and function...
  39. pmc The exosome of Trypanosoma brucei
    A M Estevez
    Zentrum fur Molekulare Biologie, Heidelberg Universitat, Im Neuenheimer Feld 282, 69120 Heidelberg, Germany
    EMBO J 20:3831-9. 2001
    ..8S rRNA maturation. We suggest that the exosome was present in primitive eukaryotes, and became increasingly complex during subsequent evolution...
  40. ncbi Antigenic variation in trypanosomes: enhanced phenotypic variation in a eukaryotic parasite
    J D Barry
    Wellcome Centre for Molecular Parasitology, University of Glasgow, Scotland, UK
    Adv Parasitol 49:1-70. 2001
    ....
  41. ncbi Isolation and characterization of subnuclear compartments from Trypanosoma brucei. Identification of a major repetitive nuclear lamina component
    M P Rout
    Laboratory of Cellular and Structural Biology, Rockefeller University, New York, New York 10021, USA
    J Biol Chem 276:38261-71. 2001
    ..Immunoelectron microscopy localized NUP-1 to the inner face of the nuclear envelope, suggesting that it is a major filamentous component of the trypanosome nuclear lamina...
  42. pmc Eukaryotic-type elongator tRNAMet of Trypanosoma brucei becomes formylated after import into mitochondria
    Timothy H P Tan
    Department of Biology Zoology, University of Fribourg, Chemin du Musee 10, CH 1700 Fribourg, Switzerland
    Proc Natl Acad Sci U S A 99:1152-7. 2002
    ..Whereas the predicted protein is homologous to prokaryotic and mitochondrial methionyl-tRNA(Met) formyltransferases, it has about twice the mass of any of these proteins...
  43. ncbi Functional characterization of the 11 non-ATPase subunit proteins in the trypanosome 19 S proteasomal regulatory complex
    Ziyin Li
    Department of Pharmaceutical Chemistry, University of California, San Francisco, California 94143 0446, USA
    J Biol Chem 277:42686-93. 2002
    ..This structural dispensability but functional indispensability of Rpn10 may constitute another unique aspect of the proteasomes in T. brucei...
  44. ncbi A putative novel nuclear-encoded subunit of the cytochrome c oxidase complex in trypanosomatids
    Dmitri A Maslov
    Department of Biology, University of California, Riverside, CA 92521, USA
    Mol Biochem Parasitol 125:113-25. 2002
    ..brucei and promastigote Leishmania mexicana amazonensis. However, the trCOIV gene, the mRNA and the polypeptide could not be detected in a respiration-deficient trypanosomatid Phytomonas serpens...
  45. ncbi Stage-specific differences in cell cycle control in Trypanosoma brucei revealed by RNA interference of a mitotic cyclin
    Tansy C Hammarton
    Wellcome Centre for Molecular Parasitology, Anderson College, University of Glasgow, 56 Dumbarton Road, Glasgow, G11 6NU, United Kingdom
    J Biol Chem 278:22877-86. 2003
    ..This indicates that there are fundamental differences in cell cycle controls between life cycle forms of T. brucei and that key cell cycle checkpoints present in higher eukaryotes are absent from trypanosomes...
  46. ncbi Characterization of Trypanosoma brucei PEX14 and its role in the import of glycosomal matrix proteins
    Juliette Moyersoen
    Research Unit for Tropical Diseases, Christian de Duve Institute of Cellular Pathology and Laboratory of Biochemistry, Universite Catholique de Louvain, Brussels, Belgium
    Eur J Biochem 270:2059-67. 2003
    ..The RNA interference experiments also showed that TbPEX14 is essential for the survival of bloodstream-form and procyclic trypanosomes. These data indicate the protein's great potential as a target for selective trypanocidal drugs...
  47. ncbi The roles of intersubunit interactions in exosome stability
    Antonio M Estevez
    Zentrum fur Molekulare Biologie, Heidelberg Universitat, Im Neuenheimer Feld 282, 69120 Heidelberg, Germany
    J Biol Chem 278:34943-51. 2003
    ..Our results are partially consistent with the proposed model of the exosome, but indicate a different arrangement of the RNase PH proteins...
  48. pmc Mitochondrial DNA ligases of Trypanosoma brucei
    Nick Downey
    Molecular Biology Institute and Department of Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles, California 90095 1570, USA
    Eukaryot Cell 4:765-74. 2005
    ..Closely related pairs of mitochondrial DNA ligase genes were also identified in Leishmania major and Crithidia fasciculata...
  49. ncbi Analysis of spliceosomal complexes in Trypanosoma brucei and silencing of two splicing factors Prp31 and Prp43
    Xue Hai Liang
    Faculty of Life Sciences, Bar Ilan University, Ramat Gan 52900, Israel
    Mol Biochem Parasitol 145:29-39. 2006
    ..We identified a 45S complex carrying pre-mRNA and all the U-snRNAs, including U1 and the SL RNA, suggesting that a single spliceosomal complex may potentially conduct both trans- and cis-splicing...
  50. ncbi Golgi duplication in Trypanosoma brucei requires Centrin2
    Cynthia Y He
    Department of Cell Biology, Yale University School of Medicine, 333 Cedar Street, New Haven, CT 06520, USA
    Science 310:1196-8. 2005
    ..Thus, a Centrin2-containing structure distinct from the basal body appears to mark the site for new Golgi assembly...
  51. pmc The F1-ATP synthase complex in bloodstream stage trypanosomes has an unusual and essential function
    Achim Schnaufer
    Seattle Biomedical Research Institute, Seattle, WA 98109 5219, USA
    EMBO J 24:4029-40. 2005
    ....
  52. pmc A "holistic" kinesin phylogeny reveals new kinesin families and predicts protein functions
    Bill Wickstead
    Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, United Kingdom
    Mol Biol Cell 17:1734-43. 2006
    ..Finally, we present a set of hidden Markov models that can reliably place most new kinesin sequences into families, even when from an organism at a great evolutionary distance from those in the analysis...
  53. ncbi Flagellar motility is required for the viability of the bloodstream trypanosome
    Richard Broadhead
    Department of Biological Sciences, Lancaster University, Lancaster LA1 4YQ, UK
    Nature 440:224-7. 2006
    ..A postgenomic meta-analysis, comparing the evolutionarily ancient trypanosome with other eukaryotes including humans, identifies numerous trypanosome-specific flagellar proteins, suggesting new avenues for selective intervention...
  54. pmc Flagellar motility contributes to cytokinesis in Trypanosoma brucei and is modulated by an evolutionarily conserved dynein regulatory system
    Katherine S Ralston
    Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, 609 Charles E Young Dr, Los Angeles, CA 90095, USA
    Eukaryot Cell 5:696-711. 2006
    ....
  55. ncbi Selective di- or trimethylation of histone H3 lysine 76 by two DOT1 homologs is important for cell cycle regulation in Trypanosoma brucei
    Christian J Janzen
    Laboratory of Molecular Parasitology, The Rockefeller University, New York, New York 10021, USA
    Mol Cell 23:497-507. 2006
    ..We propose that DOT1A and DOT1B influence the trypanosome cell cycle by regulating the degree of H3K76 methylation...
  56. ncbi Mitochondrial fatty acid synthesis in Trypanosoma brucei
    Jennifer L Stephens
    Department of Biological Chemistry, Johns Hopkins School of Medicine, Baltimore, Maryland 21205, USA
    J Biol Chem 282:4427-36. 2007
    ..Trypanosomes employ two FAS systems: the unconventional ELO pathway that synthesizes bulk fatty acids and a mitochondrial pathway that synthesizes specialized fatty acids that are likely utilized intramitochondrially...
  57. ncbi Regulated expression of glycosomal phosphoglycerate kinase in Trypanosoma brucei
    Claudia Colasante
    Zentrum für Molekulare Biologie der Universität Heidelberg ZMBH, Im Neuenheimer Feld 282, D 69120 Heidelberg, Germany
    Mol Biochem Parasitol 151:193-204. 2007
    ..We also found that production of mRNAs using T7 polymerase can affect the apparent half-life, and that large amounts of CAT enzyme may be toxic in trypanosomes...
  58. ncbi Functional genomics in Trypanosoma brucei identifies evolutionarily conserved components of motile flagella
    Desiree M Baron
    Department of Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles, CA 90095, USA
    J Cell Sci 120:478-91. 2007
    ..brucei as an experimental system for studying flagellar biology...
  59. pmc Intraflagellar transport and functional analysis of genes required for flagellum formation in trypanosomes
    Sabrina Absalon
    Trypanosome Cell Biology Unit, Pasteur Institute and Centre National de la Recherche Scientifique, 75015 Paris, France
    Mol Biol Cell 19:929-44. 2008
    ..Two PIFT proteins turned out to be required for retrograde transport and three for anterograde transport. Finally, flagellum membrane elongation continues despite the absence of axonemal microtubules in all IFT/PIFT mutant...
  60. doi Flagellum elongation is required for correct structure, orientation and function of the flagellar pocket in Trypanosoma brucei
    Sabrina Absalon
    Trypanosome Cell Biology Unit, Pasteur Institute and CNRS, 25 rue du Docteur Roux, 75015 Paris, France
    J Cell Sci 121:3704-16. 2008
    ..We propose a model to explain the role of flagellum elongation in correct flagellar pocket organisation and function...
  61. doi Functional characterization of cohesin SMC3 and separase and their roles in the segregation of large and minichromosomes in Trypanosoma brucei
    Mohamed Bessat
    Department of Biological Sciences, University of Hull, Hull, UK
    Mol Microbiol 71:1371-85. 2009
    ....
  62. pmc Differential trypanosome surface coat regulation by a CCCH protein that co-associates with procyclin mRNA cis-elements
    Pegine Walrad
    Centre for Immunology, Infection and Evolution, Institute of Immunology and Infection Research, School of Biological Sciences, Ashworth Laboratories, University of Edinburgh, Edinburgh, UK
    PLoS Pathog 5:e1000317. 2009
    ..Combined with the known association of TbZFP3 with the translational apparatus, this study provides a long-sought missing link between surface protein cis-regulatory signals and the gene expression machinery in trypanosomes...
  63. pmc A type III protein arginine methyltransferase from the protozoan parasite Trypanosoma brucei
    John C Fisk
    Department of Microbiology and Immunology, School of Medicine and Biomedical Sciences, University at Buffalo, Buffalo, New York 14124, USA
    J Biol Chem 284:11590-600. 2009
    ..Together, our studies indicate that TbPRMT7 is a Type III PRMT, and its robust activity and presence in numerous complexes suggest it plays multiple roles during the complex T. brucei life cycle...
  64. pmc Spliceosomal proteomics in Trypanosoma brucei reveal new RNA splicing factors
    Daniela Luz Ambrósio
    Department of Genetics and Developmental Biology, University of Connecticut Health Center, Farmington, Connecticut 06030 3301, USA
    Eukaryot Cell 8:990-1000. 2009
    ..Together, these data strongly indicate that most of the identified proteins are components of the spliceosome...
  65. pmc Evidence for a shared nuclear pore complex architecture that is conserved from the last common eukaryotic ancestor
    Jeffrey A DeGrasse
    Laboratory of Mass Spectrometry and Gaseous Ion Chemistry, The Rockefeller University, New York, New York 10065, USA
    Mol Cell Proteomics 8:2119-30. 2009
    ..These findings strongly support the hypothesis that NPCs share a common ancestry with vesicle coating complexes and that both were established very early in eukaryotic evolution...
  66. pmc Requirement for acetyl-CoA carboxylase in Trypanosoma brucei is dependent upon the growth environment
    Patrick A Vigueira
    Department of Biological Sciences, Clemson University, Clemson, SC 29634, USA
    Mol Microbiol 80:117-32. 2011
    ..However, ACC RNAi did attenuate virulence in a mouse model of infection. Thus the requirement for ACC in T. brucei is dependent upon the growth environment in two different life cycle stages...
  67. pmc A kinetoplastid-specific kinesin is required for cytokinesis and for maintenance of cell morphology in Trypanosoma brucei
    Liu Hu
    Department of Microbiology and Molecular Genetics, University of Texas Medical School at Houston, TX 77030, USA
    Mol Microbiol 83:565-78. 2012
    ..Our results suggest an essential role of TbKIN-C in maintaining cell morphology, likely through regulating microtubule dynamics at the posterior end of the cell...
  68. pmc Acidocalcisomes of Trypanosoma brucei have an inositol 1,4,5-trisphosphate receptor that is required for growth and infectivity
    Guozhong Huang
    Center for Tropical and Emerging Global Diseases and Department of Cellular Biology, University of Georgia, Athens, GA 30602, USA
    Proc Natl Acad Sci U S A 110:1887-92. 2013
    ....
  69. pmc A mammalian dual specificity protein kinase, Nek1, is related to the NIMA cell cycle regulator and highly expressed in meiotic germ cells
    K Letwin
    Division of Molecular and Developmental Biology, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada
    EMBO J 11:3521-31. 1992
    ..These results suggest that Nek1 is a mammalian relative of the fungal NIMA cell cycle regulator...
  70. pmc The predominant calcimedins from Trypanosoma brucei comprise a family of flagellar EF-hand calcium-binding proteins
    Y Wu
    Department of Biological Sciences, Southern Methodist University, Dallas, TX 75275
    Biochem J 287:187-93. 1992
    ..Instead, the major trypanosome calcimedins comprise a family of flagellar EF-hand calcium-binding proteins...
  71. pmc A gene from the variant surface glycoprotein expression site encodes one of several transmembrane adenylate cyclases located on the flagellum of Trypanosoma brucei
    P Paindavoine
    Department of Molecular Biology, University of Brussels, Rhode Saint Genese, Belgium
    Mol Cell Biol 12:1218-25. 1992
    ..brucei (S. Rolin, S. Halleux, J. Van Sande, J. E. Dumont, E. Pays, and M. Steinert. Exp. Parasitol. 71:350-352, 1990). Our data suggest that the trypanosome cyclases are not properly regulated in yeast cells...
  72. pmc The Trypanosoma brucei DNA polymerase alpha core subunit gene is developmentally regulated and linked to a constitutively expressed open reading frame
    P A Leegwater
    Department of Tropical Veterinary Medicine and Protozoology, University of Utrecht, The Netherlands
    Nucleic Acids Res 19:6441-7. 1991
    ..brucei DNA pol alpha core. The T.brucei pol alpha region cross-hybridizes weakly with that of T.(Nannomonas) congolense and T.(Duttonella) vivax...
  73. pmc Lack of introns in the ribosomal protein gene S14 of trypanosomes
    D Perelman
    Department of Microbiology and Immunology, Stanford University School of Medicine, California 94305 5402
    Mol Cell Biol 10:3284-8. 1990
    ..Although trans spliced, the trypanosome ribosomal protein gene S14 apparently does not have cis introns, which supports the notion that such introns are absent in this organism...
  74. ncbi Biosynthesis of the glycosyl phosphatidylinositol membrane anchor of the trypanosome variant surface glycoprotein. Origin of the non-acetylated glucosamine
    T L Doering
    Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205
    J Biol Chem 264:11168-73. 1989
    ..The GlcNAc-PI is then efficiently deacetylated to form glucosaminyl phosphatidylinositol (GlcN-PI), the substrate for subsequent reactions en route to glycolipid A...
  75. pmc Trypanosome glycosomal protein P60 is homologous to phosphoenolpyruvate carboxykinase (ATP)
    M Parsons
    Seattle Biomedical Research Institute, WA 98109
    Nucleic Acids Res 17:6411. 1989
  76. ncbi A glycosomal protein (p60) which is predominantly expressed in procyclic Trypanosoma brucei. Characterization and DNA sequence
    V Kueng
    Institut fur Allgemeine Mikrobiologie, Universitat Bern, Switzerland
    J Biol Chem 264:5203-9. 1989
    ..The biological role of the microtubule-binding capability of p60 remains unclear, whereas its membrane binding may be of physiological significance inside the glycosome...
  77. ncbi Amplification of the inosinate dehydrogenase gene in Trypanosoma brucei gambiense due to an increase in chromosome copy number
    K Wilson
    Department of Biochemistry and Molecular Biology, Oregon Health Sciences University, Portland 97201 3098
    J Biol Chem 269:28979-87. 1994
    ..brucei become resistant to cytotoxic drugs, and the amplification of the 6.0-Mb chromosome represents a novel mechanism of drug resistance in parasitic protozoa...
  78. ncbi Translational control mediates the developmental regulation of the Trypanosoma brucei Nrk protein kinase
    M Gale
    Department of Pathobiology, University of Washington, Seattle 98195
    J Biol Chem 269:31659-65. 1994
    ..Thus, Nrk is under translational control. The strict developmental regulation of the Nrk enzymes within the trypanosome life cycle suggests that the Nrk protein kinase may play a role in parasite differentiation...
  79. ncbi Molecular cloning and cellular localization of a BiP homologue in Trypanosoma brucei. Divergent ER retention signals in a lower eukaryote
    J D Bangs
    Department of Microbiology and Immunology, Stanford School of Medicine, CA 94305 5402
    J Cell Sci 105:1101-13. 1993
    ..1989) Biochemistry 28, 6440-6446), indicates that endoplasmic reticulum retrieval signals in trypanosomes may be as divergent and heterogeneous as any seen in the other eukaryotes yet studied...
  80. ncbi A ribosomal S12-like gene of Trypanosoma brucei
    C Marchal
    Department of Molecular Biology, Free University of Brussels, Rhode Saint Genese, Belgium
    Mol Biochem Parasitol 57:331-4. 1993
  81. ncbi A Trypanosoma brucei gene family encoding protein kinases with catalytic domains structurally related to Nek1 and NIMA
    M Gale
    Department of Pathobiology, University of Washington, Seattle 98195
    Mol Biochem Parasitol 59:111-21. 1993
    ..NrkA and NrkB possess multiple phosphorylation site motifs. Both nrk transcripts are constitutively expressed during parasite development...
  82. ncbi Identification of human and mouse GP-1, a putative member of a novel G-protein family
    S Senju
    Department of Neuroscience and Immunology, Kumamoto University Graduate School of Medical Sciences, Japan
    Biochem Biophys Res Commun 231:360-4. 1997
    ..GP-1 mRNA was readily detected in mouse brain, thymus, lung, and kidney, while GP-1 mRNA is rarely expressed in liver...
  83. ncbi The Trypanosoma brucei autoantigen I/6 is an internally repetitive cytoskeletal protein
    E Detmer
    Institut fur Allgemeine Mikrobiologie, Universitat Bern, Switzerland
    Eur J Cell Biol 72:378-84. 1997
    ..Protein I/6 contains a non-functional EF-hand calcium binding domain and a domain of six tandemly arranged repeat units of eight amino acids length...
  84. pmc Leishmania major parasites express cyclophilin isoforms with an unusual interaction with calcineurin
    C Rascher
    Institute of Experimental and Clinical Pharmacology and Toxicology, University of Erlangen Nurnberg, D 91054 Erlangen, Germany
    Biochem J 334:659-67. 1998
    ..These observations indicate that resistance of L. major to CsA in vitro is mediated by the lack of complex formation with calcineurin despite CsA binding by parasitic Cyp...
  85. ncbi Biochemical and molecular properties of the Trypanosoma brucei alternative oxidase
    M Chaudhuri
    Department of Microbiology, Molecular Parasitology Training Program, Meharry Medical College, Nashville, TN 37208 3599, USA
    Mol Biochem Parasitol 95:53-68. 1998
    ..Together, these points suggest that E215 plays an important role in the function of TAO. The steady state level of TAO mRNA is down-regulated in the procyclic stage presumably accounting for the low levels of TAO protein in these forms...
  86. pmc Apurinic/apyrimidinic endonuclease genes from the trypanosomatidae leishmania major and Trypanosoma cruzi confer resistance to oxidizing agents in DNA repair-deficient Escherichia coli
    J Perez
    Instituto de Parasitologia y Biomedicina Lopez Neyra, Consejo Superior de Investigaciones Cientificas, C Ventanilla 11, 18001 Granada, Spain
    Nucleic Acids Res 27:771-7. 1999
    ..This study constitutes one of the first descriptions of DNA repair enzymes in these pathogenic organisms where oxidative stress is an important mechanism of both drug-mediated and intracellular killing...
  87. ncbi Trypanosoma brucei RBP16 is a mitochondrial Y-box family protein with guide RNA binding activity
    M L Hayman
    Department of Microbiology and Center for Microbial Pathogenesis, State University of New York at Buffalo School of Medicine, Buffalo, New York 14214, USA
    J Biol Chem 274:12067-74. 1999
    ..The arginine and glycine rich C terminus in combination with the cold shock domain predicts that RBP16 will be involved in the regulation of gene expression at the posttranscriptional level...
  88. ncbi Characterization of a coiled coil protein present in the basal body of Trypanosoma brucei
    V Dilbeck
    Laboratory of Molecular Parasitology, University of Brussels, B 1640 Rhode St Gen ese, Belgium
    J Cell Sci 112:4687-94. 1999
    ..Moreover, these antibodies stained the centriole of HeLa and COS cells as well as the centriole of mouse spermatozoa, indicating that a TBBC-like centriolar component has been conserved during the evolution of eukaryotes...
  89. ncbi Characterization and disruption of a new Trypanosoma brucei repetitive flagellum protein, using double-stranded RNA inhibition
    F Bringaud
    Laboratoire de Parasitologie Moleculaire, université Victor Segalen de Bordeaux II, UMR 5016 CNRS, Bordeaux, France
    Mol Biochem Parasitol 111:283-97. 2000
    ..hrucei. Molecular ablation of FTZC does not generate any obvious phenotype such as, lethality, modification of growth rate or cellular shape, in the growth conditions used...
  90. ncbi Functional assignment of the 20 S proteasome from Trypanosoma brucei using mass spectrometry and new bioinformatics approaches
    L Huang
    Department of Pharmaceutical Chemistry, University of California, San Francisco, California 94143, USA
    J Biol Chem 276:28327-39. 2001
    ..This method resulted in sharply better statistical significance values for correct hits in the data base output relative to that achieved for independent searches using single sequence fragments...
  91. pmc Unusual organization of a developmentally regulated mitochondrial RNA polymerase (TBMTRNAP) gene in Trypanosoma brucei
    S L Clement
    Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, MI 48824, USA
    Gene 272:209-18. 2001
    ..brucei life cycle suggests that mitochondrial differentiation might be achieved in part through the regulated expression of this gene...
  92. pmc Four related proteins of the Trypanosoma brucei RNA editing complex
    A K Panigrahi
    Seattle Biomedical Research Institute, Seattle, Washington 98109, USA
    Mol Cell Biol 21:6833-40. 2001
    ..Recombinant TbMP63 and TbMP52 coimmunoprecipitate. These results indicate that these four proteins are components of the RNA editing complex and that TbMP63 and TbMP52 can interact...
  93. ncbi A developmentally regulated rab11 homologue in Trypanosoma brucei is involved in recycling processes
    T R Jeffries
    Imperial College of Science, Technology and Medicine, Department of Biochemistry, London, UK
    J Cell Sci 114:2617-26. 2001
    ..Hence, TbRAB11 is present on endosomal structures that contain recycling cargo molecules and is under developmental regulation, suggesting a role in stage-dependent endocytic processes...
  94. ncbi Trypanosoma brucei expression-site-associated-gene-8 protein interacts with a Pumilio family protein
    Maarten Hoek
    Laboratory of Molecular Parasitology, The Rockefeller University, Box 185, 1230 York Avenue, New York, NY 10021 6399, USA
    Mol Biochem Parasitol 120:269-83. 2002
    ..T. brucei is the most evolutionary divergent organism in which a Puf family protein has been identified, and our initial experiments suggest that this protein may also regulate RNA stability in trypanosomes...
  95. pmc Two related subpellicular cytoskeleton-associated proteins in Trypanosoma brucei stabilize microtubules
    Cécile Vedrenne
    Laboratoire de Parasitologie Moleculaire, université Victor Segalen de Bordeaux II, Unité Mixte Recherche 5016 Centre National de la Recherche Scientifique, 33076 Bordeaux, France
    Mol Biol Cell 13:1058-70. 2002
    ..When overexpressed in T. brucei, both CAP15 and CAP17 cover the whole subpellicular corset and induce morphological disorders, cell cycle-based abnormalities, and subsequent asymmetric cytokinesis...
  96. ncbi Ku is important for telomere maintenance, but not for differential expression of telomeric VSG genes, in African trypanosomes
    Colin Conway
    Wellcome Centre for Molecular Parasitology, University of Glasgow, Anderson College, 56 Dumbarton Road, Glasgow, G11 6NU, Scotland, United Kingdom
    J Biol Chem 277:21269-77. 2002
    ..The absence of Ku and the consequent great shortening of telomeres had no detectable influence either on the rate of VSG switching or on the silencing of the telomeric promoters of the VSG subset that is expressed in the tsetse fly...
  97. ncbi Sequencing, modeling, and selective inhibition of Trypanosoma brucei hexokinase
    Michele Willson
    Groupe de Chimie Organique Biologique, Laboratoire Synthèse Physico Chimie des Molécules d Intérêt Biologique, UMR CNRS 5068, Universite Paul Sabatier, 31062 Cedex, Toulouse, France
    Chem Biol 9:839-47. 2002
    ....
  98. pmc GPI transamidase of Trypanosoma brucei has two previously uncharacterized (trypanosomatid transamidase 1 and 2) and three common subunits
    Kisaburo Nagamune
    Department of Immunoregulation, Research Institute for Microbial Diseases, Osaka University, 3 1 Yamada oka, Suita, Osaka 565 0871, Japan
    Proc Natl Acad Sci U S A 100:10682-7. 2003
    ....
  99. pmc A chromosomal SIR2 homologue with both histone NAD-dependent ADP-ribosyltransferase and deacetylase activities is involved in DNA repair in Trypanosoma brucei
    Jose A Garcia-Salcedo
    Institute of Molecular Biology and Medicine, Free University of Brussels, 12 rue des Professeurs Jeener et Brachet, B 6041 Gosselies, Belgium
    EMBO J 22:5851-62. 2003
    ..Both of these responses correlated with the level of TbSIR2RP1 expression. We propose that histone modification by TbSIR2RP1 is involved in DNA repair...
  100. ncbi Trypanosoma brucei plasma membrane-type Ca(2+)-ATPase 1 (TbPMC1) and 2 (TbPMC2) genes encode functional Ca(2+)-ATPases localized to the acidocalcisomes and plasma membrane, and essential for Ca(2+) homeostasis and growth
    Shuhong Luo
    Department of Pathobiology, and Center for Zoonoses Research, University of Illinois at Urbana Champaign, Urbana, Illinois 61802, USA
    J Biol Chem 279:14427-39. 2004
    ..These results establish that T. brucei PMCA-Ca(2+)-ATPases are essential for parasite viability and validate them as targets for drug development...
  101. ncbi Identification and functional characterization of lsm proteins in Trypanosoma brucei
    Qing Liu
    Faculty of Life Sciences, Bar Ilan University, Ramat Gan 52900, Israel
    J Biol Chem 279:18210-9. 2004
    ..These data therefore suggest that in trypanosomes only a single Lsm complex may exist...

Research Grants7

  1. EXPANDED PROTEIN STRUCTURE REFINEMENT AND VALIDATION
    Ethan Merritt; Fiscal Year: 2005
    ..This, in turn, will make it easier to identify key biological features such as the presence and conformation of bound ligands, and the nature of hinge and inter-domain motions. ..
  2. Software tools for analysis and visulaization of protein structure
    Ethan A Merritt; Fiscal Year: 2010
    ..The algorithms and computation tools developed as part of this work will be distributed freely, and we will provide web-based state of the art analysis of structures submitted by external researchers. ..
  3. A NOVEL MICROTUBULE-ASSOCIATED PROTEIN IN TRYPANOSOMES
    KENT HILL; Fiscal Year: 2002
    ....
  4. MITOCHONDRIAL PROTEIN IMPORT IN AFRICAN TRYPANOSOMES
    Minu Chaudhuri; Fiscal Year: 2002
    ..Understanding of this developmental process will elucidate one of the major aspects of trypanosomal parasitism. This in turn will help us to design rational trypanocidal chemotherapeutic strategies. ..
  5. Sterol Biosynthesis in Trypanosomatid Parasites
    Frederick Buckner; Fiscal Year: 2004
    ..The drugs discovered in this research program will hopefully provide better future treatment for patients with these devastating diseases. ..
  6. A High Throughput Assay to Identify Onhibitors of Trypanosome RNA Editing Ligase
    Nancy Ernst; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  7. Biochemical and Functional Analysis of Trypanin
    KENT HILL; Fiscal Year: 2007
    ..Therefore, in addition to addressing fundamental questions in cell biology, this research directly impacts efforts to understand and treat infectious diseases and genetic diseases in humans. ..