Mycobacterium tuberculosis H37Rv

Summary

Alias: Mycobacterium tuberculosis str. H37Rv, Mycobacterium tuberculosis strain H37Rv

Top Publications

  1. ncbi Mycobacterium tuberculosis H37Rv ESAT-6-CFP-10 complex formation confers thermodynamic and biochemical stability
    Akshaya K Meher
    Molecular and Structural Biology, Central Drug Research Institute, Lucknow, India
    FEBS J 273:1445-62. 2006
  2. pmc Contribution of the Mycobacterium tuberculosis MmpL protein family to virulence and drug resistance
    Pilar Domenech
    Tuberculosis Research Section, Laboratory of Immunogenetics, 12441 Parklawn Drive, Rockville, MD 20852, USA
    Infect Immun 73:3492-501. 2005
  3. pmc Characterization of a Clp protease gene regulator and the reaeration response in Mycobacterium tuberculosis
    Ashley M Sherrid
    Seattle Biomedical Research Institute, Seattle, Washington, United States of America
    PLoS ONE 5:e11622. 2010
  4. ncbi DnaE2 polymerase contributes to in vivo survival and the emergence of drug resistance in Mycobacterium tuberculosis
    Helena I M Boshoff
    Laboratory of Host Defenses, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Twinbrook II, 12441 Parklawn Drive, Rockville, MD 20852, USA
    Cell 113:183-93. 2003
  5. pmc Mycobacterium tuberculosis nuoG is a virulence gene that inhibits apoptosis of infected host cells
    Kamalakannan Velmurugan
    Department of Cell Biology and Molecular Genetics, University of Maryland, College Park, Maryland, United States of America
    PLoS Pathog 3:e110. 2007
  6. pmc Activity-based metabolomic profiling of enzymatic function: identification of Rv1248c as a mycobacterial 2-hydroxy-3-oxoadipate synthase
    Luiz Pedro S de Carvalho
    Department of Microbiology and Immunology, Weill Cornell Medical College, 1300 York Avenue, New York, NY 10065, USA
    Chem Biol 17:323-32. 2010
  7. pmc Extensive phosphorylation with overlapping specificity by Mycobacterium tuberculosis serine/threonine protein kinases
    Sladjana Prisic
    Division of Infectious Diseases, Children s Hospital Boston, Harvard Medical School, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 107:7521-6. 2010
  8. ncbi Direct extracellular interaction between the early secreted antigen ESAT-6 of Mycobacterium tuberculosis and TLR2 inhibits TLR signaling in macrophages
    Sushil Kumar Pathak
    Bose Institute, Department of Chemistry, Kolkata 700 009, India
    Nat Immunol 8:610-8. 2007
  9. ncbi The ESAT6 protein of Mycobacterium tuberculosis induces apoptosis of macrophages by activating caspase expression
    Steven C Derrick
    Laboratory of Mycobacterial Diseases and Cellular Immunology, Center for Biologics Evaluation and Research, United States Food and Drug Administration, Bethesda, MD 20892, USA
    Cell Microbiol 9:1547-55. 2007
  10. pmc Identification of Mycobacterium tuberculosis RNAs synthesized in response to phagocytosis by human macrophages by selective capture of transcribed sequences (SCOTS)
    J E Graham
    Department of Biology, Washington University, St Louis, MO 63130, USA
    Proc Natl Acad Sci U S A 96:11554-9. 1999

Research Grants

  1. Screen for Mycobacterium Tuberculosis (RMI)
    E White; Fiscal Year: 2005
  2. Regulation of Cellular division in M. tuberculosis
    RICHARD SLAYDEN; Fiscal Year: 2006
  3. MYCOBACTERIAL ENVIRONMENT WITHIN MACROPHAGES
    Luiz Bermudez; Fiscal Year: 2003
  4. Post Translation Regulation in Mycobacteria
    Luiz Bermudez; Fiscal Year: 2008
  5. Reactivation Tuberculosis in A/J
    Chinnaswamy Jagannath; Fiscal Year: 2005
  6. Mtb Proteasome-Associated Factors and Virulence
    KATERINA DARWIN; Fiscal Year: 2007
  7. Structural Studies of AIDS-Responsive Drugs
    Vivian Cody; Fiscal Year: 2010
  8. Genetic screens to identify mycobacterial porins
    Martin Pavelka; Fiscal Year: 2008
  9. Identification of secreted proteins important for tularemia pathogenesis
    Martin Pavelka; Fiscal Year: 2007
  10. Identification of genes controlling TB in murine lungs
    Alexander Apt; Fiscal Year: 2005

Patents

  1. ENZYMATIC SYSTEM-CONTAINING COSMETIC COMPOSITIONS
  2. ENZYMATIC SYSTEM-CONTAINING COSMETIC COMPOSITIONS
  3. MYCOBACTERIUM ANTIGENIC COMPOSITION
  4. DIAGNOSIS AND TREATMENT OF MULTIPLE SULFATASE DEFICIENCY AND OTHER SULFATASE DEFICIENCIES
  5. Diagnosis and treatment of multiple sulfatase deficiency and other using a formylglycine generating enzyme (fge)
  6. Highly conserved genes and their use to generate probes and primers for detection of microorganisms
  7. Proteins associated with abiotic stress response and homologs
  8. Reaction or detection tubular container, and reagent kit comprising said container
  9. COMPUTATIONAL METHOD FOR IDETIFYING ADHESIN
  10. RECOMBINANT HOST CELLS COMPRISING PHOSPHOKETOLASES

Detail Information

Publications341 found, 100 shown here

  1. ncbi Mycobacterium tuberculosis H37Rv ESAT-6-CFP-10 complex formation confers thermodynamic and biochemical stability
    Akshaya K Meher
    Molecular and Structural Biology, Central Drug Research Institute, Lucknow, India
    FEBS J 273:1445-62. 2006
    ..ESAT-6) and culture filtrate protein-10 (CFP-10), expressed from the region of deletion-1 (RD1) of Mycobacterium tuberculosis H37Rv, are known to play a key role in virulence...
  2. pmc Contribution of the Mycobacterium tuberculosis MmpL protein family to virulence and drug resistance
    Pilar Domenech
    Tuberculosis Research Section, Laboratory of Immunogenetics, 12441 Parklawn Drive, Rockville, MD 20852, USA
    Infect Immun 73:3492-501. 2005
    ....
  3. pmc Characterization of a Clp protease gene regulator and the reaeration response in Mycobacterium tuberculosis
    Ashley M Sherrid
    Seattle Biomedical Research Institute, Seattle, Washington, United States of America
    PLoS ONE 5:e11622. 2010
    ..In sum, this study defines a new transcriptional response of MTB with potential relevance to disease, and implicates ClgR as a regulator involved in resumption of replication following hypoxia...
  4. ncbi DnaE2 polymerase contributes to in vivo survival and the emergence of drug resistance in Mycobacterium tuberculosis
    Helena I M Boshoff
    Laboratory of Host Defenses, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Twinbrook II, 12441 Parklawn Drive, Rockville, MD 20852, USA
    Cell 113:183-93. 2003
    ..These results may indicate a potential new target for therapeutic intervention...
  5. pmc Mycobacterium tuberculosis nuoG is a virulence gene that inhibits apoptosis of infected host cells
    Kamalakannan Velmurugan
    Department of Cell Biology and Molecular Genetics, University of Maryland, College Park, Maryland, United States of America
    PLoS Pathog 3:e110. 2007
    ..tuberculosis and support a direct causal relationship between virulence of pathogenic mycobacteria and their ability to inhibit macrophage apoptosis...
  6. pmc Activity-based metabolomic profiling of enzymatic function: identification of Rv1248c as a mycobacterial 2-hydroxy-3-oxoadipate synthase
    Luiz Pedro S de Carvalho
    Department of Microbiology and Immunology, Weill Cornell Medical College, 1300 York Avenue, New York, NY 10065, USA
    Chem Biol 17:323-32. 2010
    ..Thus, Rv1248c encodes an HOA synthase...
  7. pmc Extensive phosphorylation with overlapping specificity by Mycobacterium tuberculosis serine/threonine protein kinases
    Sladjana Prisic
    Division of Infectious Diseases, Children s Hospital Boston, Harvard Medical School, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 107:7521-6. 2010
    ..tuberculosis and create a resource for understanding how specific phosphorylation events modulate protein activity. The results further provide the potential to predict likely cognate STPKs for newly identified phosphoproteins...
  8. ncbi Direct extracellular interaction between the early secreted antigen ESAT-6 of Mycobacterium tuberculosis and TLR2 inhibits TLR signaling in macrophages
    Sushil Kumar Pathak
    Bose Institute, Department of Chemistry, Kolkata 700 009, India
    Nat Immunol 8:610-8. 2007
    ....
  9. ncbi The ESAT6 protein of Mycobacterium tuberculosis induces apoptosis of macrophages by activating caspase expression
    Steven C Derrick
    Laboratory of Mycobacterial Diseases and Cellular Immunology, Center for Biologics Evaluation and Research, United States Food and Drug Administration, Bethesda, MD 20892, USA
    Cell Microbiol 9:1547-55. 2007
    ..Finally, experimental results using a cell impermeable fluorescent stain suggests that the formation of membrane pores may be a primary mechanism by which ESAT6 evokes an apoptotic response...
  10. pmc Identification of Mycobacterium tuberculosis RNAs synthesized in response to phagocytosis by human macrophages by selective capture of transcribed sequences (SCOTS)
    J E Graham
    Department of Biology, Washington University, St Louis, MO 63130, USA
    Proc Natl Acad Sci U S A 96:11554-9. 1999
    ..tuberculosis genes expressed in response to host interaction, will allow the study of gene expression in a variety of microorganisms, including expression resulting from interaction with human tissues in natural disease states...
  11. pmc Mycobacterium tuberculosis WhiB3 responds to O2 and nitric oxide via its [4Fe-4S] cluster and is essential for nutrient starvation survival
    Amit Singh
    Department of Microbiology, University of Alabama at Birmingham, Birmingham, AL 35294, USA
    Proc Natl Acad Sci U S A 104:11562-7. 2007
    ..Taken together, our results suggest that WhiB3 is an intracellular redox sensor that integrates environmental redox signals with core intermediary metabolism...
  12. ncbi Polyphosphate kinase is involved in stress-induced mprAB-sigE-rel signalling in mycobacteria
    Kamakshi Sureka
    Department of Chemistry, Bose Institute, 93 1 Acharya Prafulla Chandra Road, Calcutta 700009, India
    Mol Microbiol 65:261-76. 2007
    ..smegmatis and M. tuberculosis. Downregulation of ppk1 led to impaired survival of M. tuberculosis in macrophages. PolyP plays a central role in the stress response of mycobacteria...
  13. pmc Mycobacterium tuberculosis WhiB1 is an essential DNA-binding protein with a nitric oxide-sensitive iron-sulfur cluster
    Laura J Smith
    Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield S10 2TN, UK
    Biochem J 432:417-27. 2010
    ..A model incorporating regulation of whiB1 expression in response to nitric oxide and cAMP is discussed with implications for sensing two important signals in establishing M. tuberculosis infections...
  14. pmc mmr, a Mycobacterium tuberculosis gene conferring resistance to small cationic dyes and inhibitors
    E De Rossi
    Department of Genetics and Microbiology, University of Pavia, 27100 Pavia, Italy
    J Bacteriol 180:6068-71. 1998
    ..The gene appears to code for a protein containing four transmembrane domains. Studies of [3H]TPP intracellular accumulation strongly suggest that the resistance mediated by the Mmr protein involves active extrusion of TPP...
  15. ncbi The Mycobacterium tuberculosis pks2 gene encodes the synthase for the hepta- and octamethyl-branched fatty acids required for sulfolipid synthesis
    T D Sirakova
    Neurobiotechnology Center and Department of Biochemistry, The Ohio State University, Columbus, Ohio 43210, USA
    J Biol Chem 276:16833-9. 2001
    ..With this sulfolipid-deficient mutant, it should be possible to test for the postulated important roles for sulfolipids in the pathogenesis of M. tuberculosis...
  16. ncbi NAD+-dependent DNA Ligase (Rv3014c) from Mycobacterium tuberculosis. Crystal structure of the adenylation domain and identification of novel inhibitors
    Sandeep Kumar Srivastava
    Division Molecular and Structural Biology, Central Drug Research Institute, Chattar Manzil, Mahatma Gandhi Marg, Lucknow 226001, India
    J Biol Chem 280:30273-81. 2005
    ..The results can be used as the basis for rational design of novel antibacterial agents...
  17. ncbi Insights into the inter-ring plasticity of caseinolytic proteases from the X-ray structure of Mycobacterium tuberculosis ClpP1
    Henrik Ingvarsson
    Department of Cell and Molecular Biology, Uppsala University, Biomedical Center, Box 596, SE 751 24 Uppsala, Sweden
    Acta Crystallogr D Biol Crystallogr 63:249-59. 2007
    ..The models also reveal structural differences within the N-terminal hairpin-loop domain, which possibly reflect the significant differences in amino-acid sequence between Mt ClpP1 and other ClpP homologues in this region...
  18. pmc Lsr2 of Mycobacterium represents a novel class of H-NS-like proteins
    Blair R G Gordon
    Department of Molecular Genetics, University of Toronto, 1 King s College Circle, Toronto ON M5S 1A8, Canada
    J Bacteriol 190:7052-9. 2008
    ..Taken together, our results demonstrate unequivocally that Lsr2 is an H-NS-like protein...
  19. pmc Functional genomics reveals extended roles of the Mycobacterium tuberculosis stress response factor sigmaH
    Smriti Mehra
    Division of Bacteriology and Parasitology, Tulane National Primate Research Center, Covington, LA 70433, USA
    J Bacteriol 191:3965-80. 2009
    ..sigma(H) caused the induction of the ATP-dependent clp proteolysis regulon, likely mediated by a transcription factor encoded by Rv2745c, several members of the mce1 virulence regulon, and the sulfate acquisition/transport network...
  20. pmc Forkhead-associated domain-containing protein Rv0019c and polyketide-associated protein PapA5, from substrates of serine/threonine protein kinase PknB to interacting proteins of Mycobacterium tuberculosis
    Meetu Gupta
    Institute of Genomics and Integrative Biology, Council of Scientific and Industrial Research, Delhi 110007, India
    J Biol Chem 284:34723-34. 2009
    ..Thus, our data provides initial clues for a possible regulation of PapA5 and hence the phthiocerol dimycocerosate biosynthesis by PknB, either by direct phosphorylation of PapA5 or indirectly through Rv0019c...
  21. ncbi Mycobacterium tuberculosis ribose-5-phosphate isomerase has a known fold, but a novel active site
    Annette K Roos
    Department of Cell and Molecular Biology, Uppsala University, Biomedical Center, Box 596, SE 751 24 Uppsala, Sweden
    J Mol Biol 335:799-809. 2004
    ..Docking studies allow additional insights into the reactions of all three enzymes, and show that many features of the mechanism are preserved despite the different catalytic components...
  22. pmc A member of the cAMP receptor protein family of transcription regulators in Mycobacterium tuberculosis is required for virulence in mice and controls transcription of the rpfA gene coding for a resuscitation promoting factor
    Lisa Rickman
    Division of Mycobacterial Research, National Institute for Medical Research, Mill Hill, London NW7 1AA, UK
    Mol Microbiol 56:1274-86. 2005
    ..In summary, it has been shown that Rv3676 is a direct regulator of rpfA expression, and because rpfA codes for a resuscitation promoting factor this may implicate Rv3676 in reactivation of dormant M. tuberculosis infections...
  23. pmc Co-expression of DevR and DevR(N)-Aph proteins is associated with hypoxic adaptation defect and virulence attenuation of Mycobacterium tuberculosis
    Shyamasree De Majumdar
    Department of Biotechnology, All India Institute of Medical Sciences, New Delhi, India
    PLoS ONE 5:e9448. 2010
    ..DevR regulon genes are powerfully induced in vivo implicating them in bacterial adaptation to host control strategies. A better understanding of DevR function will illumine the way for new strategies to control and treat tuberculosis...
  24. pmc The characterization of conserved binding motifs and potential target genes for M. tuberculosis MtrAB reveals a link between the two-component system and the drug resistance of M. smegmatis
    Yuqing Li
    National Key Laboratory of Agricultural Microbiology, Center for Proteomics Research, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China
    BMC Microbiol 10:242. 2010
    ..tuberculosis replication initiator gene, dnaA. However, the dnaA promoter binding sites and many potential target genes for MtrA have yet to be precisely characterized...
  25. pmc Mycobacterium tuberculosis MycP1 protease plays a dual role in regulation of ESX-1 secretion and virulence
    Yamini M Ohol
    Department of Microbiology and Immunology, University of California, San Francisco, San Francisco, CA 94158, USA
    Cell Host Microbe 7:210-20. 2010
    ..As the key ESX-1 substrates ESAT-6 and CFP-10 are highly immunogenic, fine-tuning of their secretion by MycP1 may balance virulence and immune detection and be essential for successful maintenance of long-term M. tuberculosis infection...
  26. ncbi Diarylquinolines target subunit c of mycobacterial ATP synthase
    Anil Koul
    Department of Antimicrobial Research, Tibotec BVBA, Johnson and Johnson, Turnhoutseweg 30, B 2340 Beerse, Belgium
    Nat Chem Biol 3:323-4. 2007
    ..Here we establish by genetic, biochemical and binding assays that the oligomeric subunit c (AtpE) of ATP synthase is the target of R207910. Thus targeting energy metabolism is a new, promising approach for antibacterial drug discovery...
  27. ncbi Mutations found in the pncA gene of Mycobacterium tuberculosis in clinical pyrazinamide-resistant isolates from a local region of China
    H Zhang
    National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China
    J Int Med Res 37:1430-5. 2009
    ..These results show the molecular mechanism of pyrazinamide resistance in China and may also contribute towards the prevention of tuberculosis in China...
  28. pmc Conserved Pro-Glu (PE) and Pro-Pro-Glu (PPE) protein domains target LipY lipases of pathogenic mycobacteria to the cell surface via the ESX-5 pathway
    Maria H Daleke
    Department of Medical Microbiology and Infection Control, VU University Medical Centre, 1081 BT Amsterdam, The Netherlands
    J Biol Chem 286:19024-34. 2011
    ..In conclusion, both PE and PPE domains contain a signal required for secretion of LipY by the ESX-5 system, and these domains are proteolytically removed upon translocation...
  29. pmc Protective immunity against tuberculosis induced by vaccination with major extracellular proteins of Mycobacterium tuberculosis
    M A Horwitz
    Department of Medicine, School of Medicine, University of California, Los Angeles 90024
    Proc Natl Acad Sci U S A 92:1530-4. 1995
    ..tuberculosis are candidate components of a subunit vaccine against tuberculosis and provides compelling support for the concept that extracellular proteins of intracellular pathogens are key immunoprotective molecules...
  30. pmc A riboswitch regulates expression of the coenzyme B12-independent methionine synthase in Mycobacterium tuberculosis: implications for differential methionine synthase function in strains H37Rv and CDC1551
    Digby F Warner
    Molecular Mycobacteriology Research Unit, National Health Laboratory Service, P O Box 1038, Johannesburg 2000, South Africa
    J Bacteriol 189:3655-9. 2007
    ..Expression analysis confirmed that the B(12) riboswitch is a transcriptional regulator of metE in M. tuberculosis...
  31. pmc Secreted Mycobacterium tuberculosis Rv3654c and Rv3655c proteins participate in the suppression of macrophage apoptosis
    Lia Danelishvili
    Department of Biomedical Sciences, College of Veterinary Medicine, Oregon State University, Corvallis, Oregon, United States of America
    PLoS ONE 5:e10474. 2010
    ..The mechanisms by which M. tuberculosis H37Rv strain suppress apoptosis and escapes human macrophage killing was investigated...
  32. doi Insights into the function of the WhiB-like protein of mycobacteriophage TM4--a transcriptional inhibitor of WhiB2
    Jan Rybniker
    1st Department of Internal Medicine, Division of Infectious Diseases, 50924 Cologne, Germany
    Mol Microbiol 77:642-57. 2010
    ..Thus, we provide substantial evidence supporting the hypothesis of viral and bacterial WhiB proteins being important Fe-S containing transcriptional regulators with DNA-binding capability...
  33. pmc Copper resistance is essential for virulence of Mycobacterium tuberculosis
    Frank Wolschendorf
    Department of Microbiology, University of Alabama at Birmingham, Birmingham, AL 35294, USA
    Proc Natl Acad Sci U S A 108:1621-6. 2011
    ..Hence, this study reveals an Achilles heel of Mtb that might be a promising target for tuberculosis chemotherapy...
  34. pmc Definition of the mycobacterial SOS box and use to identify LexA-regulated genes in Mycobacterium tuberculosis
    Elaine O Davis
    Division of Mycobacterial Research, National Institute for Medical Research, London NW7 1AA, England
    J Bacteriol 184:3287-95. 2002
    ..Curiously, of the remaining 10 genes predicted to be LexA regulated, 7 are members of the M. tuberculosis 13E12 repeat family, which has some of the characteristics of mobile elements...
  35. pmc Molecular genetic analysis of nucleotide polymorphisms associated with ethambutol resistance in human isolates of Mycobacterium tuberculosis
    S V Ramaswamy
    Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana 59840, USA
    Antimicrob Agents Chemother 44:326-36. 2000
    ..Taken together, the results indicate that there are multiple molecular pathways to the EMB resistance phenotype...
  36. ncbi Modulation of Mycobacterium tuberculosis proliferation by MtrA, an essential two-component response regulator
    Marek Fol
    Biomedical Research, The University of Texas Health Center at Tyler, 11937 U S Hwy 271, Tyler, TX 75708 3154, USA
    Mol Microbiol 60:643-57. 2006
    ..We propose that proliferation of M. tuberculosis in vivo depends, in part, on the optimal ratio of phosphorylated to non-phosphorylated MtrA response regulator...
  37. pmc Mycobacterium tuberculosis HBHA protein reacts strongly with the serum immunoglobulin M of tuberculosis patients
    A Rum Shin
    Department of Microbiology, College of Medicine, Chungnam National University, 6 Muwha Dong, Jung ku, Daejeon 301 747, Korea
    Clin Vaccine Immunol 13:869-75. 2006
    ..tuberculosis into epithelial cells. These findings suggest that IgM antibody to HBHA may play a role in protection against extrapulmonary dissemination...
  38. pmc Unique roles of DosT and DosS in DosR regulon induction and Mycobacterium tuberculosis dormancy
    Ryan W Honaker
    University of Colorado Denver, Department of Microbiology, Aurora, CO 80045, USA
    Infect Immun 77:3258-63. 2009
    ..Thus, M. tuberculosis has evolved a system whereby it responds to hypoxic conditions in a stepwise fashion as it enters an anaerobic state...
  39. pmc Overexpression of the chromosomally encoded aminoglycoside acetyltransferase eis confers kanamycin resistance in Mycobacterium tuberculosis
    M Analise Zaunbrecher
    Microbiology and Molecular Genetics Graduate Program, Department of Microbiology and Immunology, Rollins Research Center, Emory University, Atlanta, GA 30322, USA
    Proc Natl Acad Sci U S A 106:20004-9. 2009
    ..This may help avoid excluding a potentially effective drug from a treatment regimen for drug-resistant TB...
  40. pmc Characterization of the major membrane protein of virulent Mycobacterium tuberculosis
    B Y Lee
    Department of Microbiology, Colorado State University, Fort Collins 80523
    Infect Immun 60:2066-74. 1992
    ..Immunoblotting indicated that this protein is highly expressed in the virulent strains of M. tuberculosis. Its application to sera from patients with pulmonary tuberculosis showed promise as a serodiagnostic tool...
  41. doi Genome-wide regulon and crystal structure of BlaI (Rv1846c) from Mycobacterium tuberculosis
    Claudia Sala
    Global Health Institute, Ecole Polytechnique Federale de Lausanne, CH 1015 Lausanne, Switzerland
    Mol Microbiol 71:1102-16. 2009
    ..The existence of a potential regulatory loop between cell wall integrity and ATP production was previously unknown...
  42. ncbi Modification of the NADH of the isoniazid target (InhA) from Mycobacterium tuberculosis
    D A Rozwarski
    Department of Biochemistry and Biophysics, Texas A and M University, College Station, TX 77843, USA
    Science 279:98-102. 1998
    ....
  43. pmc Identification and characterization of a regulatory sequence recognized by Mycobacterium tuberculosis persistence regulator MprA
    Hongjun He
    Department of Microbiology and Molecular Genetics, Medical College of Wisconsin, 8701 Watertown Plank Rd, P O Box 26509, Milwaukee, WI 53226, USA
    J Bacteriol 187:202-12. 2005
    ..Identification of the genetic determinants regulated by MprA will ultimately enhance our understanding of the mechanisms utilized by M. tuberculosis to undergo latency...
  44. ncbi Crystallographic studies of shikimate binding and induced conformational changes in Mycobacterium tuberculosis shikimate kinase
    Balvinder Dhaliwal
    Division of Structural Biology, The Wellcome Trust Centre for Human Genetics, University of Oxford, Roosevelt Drive, Oxford OX3 7BN, UK
    FEBS Lett 574:49-54. 2004
    ..Detailed knowledge of shikimate binding is an important step in the design of inhibitors of SK, which have potential as novel anti-tuberculosis agents...
  45. pmc Phosphorylation of mycobacterial PcaA inhibits mycolic acid cyclopropanation: consequences for intracellular survival and for phagosome maturation block
    Rosa Milagros Corrales
    Laboratoire de Dynamique des Interactions Membranaires Normales et Pathologiques, Universités de Montpellier II et I, CNRS, UMR 5235, Case 107, Place Eugene Bataillon, 34095 Montpellier Cedex 05, France
    J Biol Chem 287:26187-99. 2012
    ..Our results add new insight into the importance of mycolic acid cyclopropane rings in the PMB and provide the first evidence of a Ser/Thr kinase-dependent mechanism for modulating mycolic acid composition and PMB...
  46. pmc Mycobacterium tuberculosis thymidylate synthase gene thyX is essential and potentially bifunctional, while thyA deletion confers resistance to p-aminosalicylic acid
    Amanda S Fivian-Hughes
    Division of Mycobacterial Research, MRC National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, UK
    Microbiology 158:308-18. 2012
    ..We also demonstrate that thyA deletion confers M. tuberculosis p-aminosalicylic acid resistance, and show by complementation studies that ThyA T202A and V261G appear to be functional and non-functional, respectively...
  47. ncbi Mycobacterium tuberculosis beta-ketoacyl-ACP reductase: alpha-secondary kinetic isotope effects and kinetic and equilibrium mechanisms of substrate binding
    Rafael G Silva
    Centro de Pesquisas em Biologia Molecular e Funcional, Faculdade de Biociências e Faculdade de Farmácia, Instituto de Pesquisas Biomédicas, PUCRS, 6681 92 A Av Ipiranga, 90619 900, Porto Alegre, RS, Brazil
    Arch Biochem Biophys 471:1-10. 2008
    ..Moreover, modest affinity loss occurs when the substrates bind to the monomer as compared to the dimer of MabA. A mechanism of substrate binding to MabA is proposed on the basis of the experimental data...
  48. pmc Kinetics and ligand-binding preferences of Mycobacterium tuberculosis thymidylate synthases, ThyA and ThyX
    Joshua H Hunter
    Department of Chemistry, University of Washington, Seattle, Washington, United States of America
    PLoS ONE 3:e2237. 2008
    ..To facilitate future small molecule inhibitors against these proteins, a detailed enzymatic characterization was necessary...
  49. pmc Expression, essentiality, and a microtiter plate assay for mycobacterial GlmU, the bifunctional glucosamine-1-phosphate acetyltransferase and N-acetylglucosamine-1-phosphate uridyltransferase
    Wenli Zhang
    Department of Biochemistry and Molecular Biology, Dalian Medical University, Dalian 116044, China
    Int J Biochem Cell Biol 40:2560-71. 2008
    ..In Mycobacterium tuberculosis H37Rv genome, Rv1018c shows strong homology to the GlmU protein involved in the formation of UDP-GlcNAc ..
  50. pmc Genetic evaluation of relationship between mutations in rpoB and resistance of Mycobacterium tuberculosis to rifampin
    Anna Zaczek
    Institute for Medical Biology, Polish Academy of Sciences, Lodz, Poland
    BMC Microbiol 9:10. 2009
    ..Among many mutations identified in the rpoB gene, few were verified by molecular genetic methods as responsible for resistance to rifampin (RMP)...
  51. pmc Mycobacterium tuberculosis PhoP recognizes two adjacent direct-repeat sequences to form head-to-head dimers
    Sankalp Gupta
    Institute of Microbial Technology, Sector 39A, Chandigarh 160036, India
    J Bacteriol 191:7466-76. 2009
    ....
  52. pmc M. tuberculosis intramembrane protease Rip1 controls transcription through three anti-sigma factor substrates
    Joseph G Sklar
    Immunology Program, Sloan Kettering Institute, New York, NY 10021, USA
    Mol Microbiol 77:605-17. 2010
    ....
  53. pmc The biological and structural characterization of Mycobacterium tuberculosis UvrA provides novel insights into its mechanism of action
    Franca Rossi
    DiSCAFF, University of Piemonte Orientale Amedeo Avogadro, Via Bovio 6, 28100 Novara, Italy
    Nucleic Acids Res 39:7316-28. 2011
    ..tuberculosis...
  54. ncbi The Mycobacterium tuberculosis iniA gene is essential for activity of an efflux pump that confers drug tolerance to both isoniazid and ethambutol
    Roberto Colangeli
    Division of Infectious Disease and the Center for Emerging Pathogens, Department of Medicine, New Jersey Medical School, University of Medicine and Dentistry of New Jersey, Newark, NJ 07103, USA
    Mol Microbiol 55:1829-40. 2005
    ..Drugs designed to inhibit the iniA gene product may shorten the time required to treat tuberculosis and may help prevent the clinical emergence of drug resistance...
  55. ncbi Expression of the PE_PGRS 33 protein in Mycobacterium smegmatis triggers necrosis in macrophages and enhanced mycobacterial survival
    Veerabadran Dheenadhayalan
    Laboratory of Mycobacterial Diseases and Cellular Immunology, Center for Biologics Evaluation and Research, Food and Drug Administration, Building 29, Room 503, HFM 431, 29 Lincoln Drive, Bethesda, MD 20892, USA
    Microbes Infect 8:262-72. 2006
    ....
  56. pmc Deletion of the Mycobacterium tuberculosis alpha-crystallin-like hspX gene causes increased bacterial growth in vivo
    Yanmin Hu
    Medical Microbiology, Department of Cellular and Molecular Sciences, St George s Hospital Medical School, London SW17 0RE, United Kingdom
    Infect Immun 74:861-8. 2006
    ..We also confirmed that constitutive expression of hspX slows growth in vitro, and we propose that hspX plays an active role in slowing the growth of M. tuberculosis in vivo immediately following infection...
  57. pmc Crystal structures of the response regulator DosR from Mycobacterium tuberculosis suggest a helix rearrangement mechanism for phosphorylation activation
    Goragot Wisedchaisri
    Department of Biochemistry, University of Washington, Seattle, Washington 98195, USA
    J Mol Biol 378:227-42. 2008
    ..Our structures suggest a mode of DosR activation by phosphorylation via a helix rearrangement mechanism...
  58. pmc Characterization of active site structure in CYP121. A cytochrome P450 essential for viability of Mycobacterium tuberculosis H37Rv
    Kirsty J McLean
    Manchester Interdisciplinary Biocentre, Faculty of Life Sciences, University of Manchester, 131 Princess Street, Manchester M1 7DN, United Kingdom
    J Biol Chem 283:33406-16. 2008
    ..Collectively, data point to an important cellular role for CYP121 and highlight its potential as a novel Mtb drug target...
  59. pmc Powerful induction of divergent tgs1-Rv3131 genes in Mycobacterium tuberculosis is mediated by DevR interaction with a high-affinity site and an adjacent cryptic low-affinity site
    Santosh Chauhan
    Department of Biotechnology, All India Institute of Medical Sciences, New Delhi, India
    J Bacteriol 191:6075-81. 2009
    ....
  60. doi embCAB sequence variation among ethambutol-resistant Mycobacterium tuberculosis isolates without embB306 mutation
    Claudia Plinke
    Molecular Mycobacteriology, Research Center Borstel, Borstel, Germany
    J Antimicrob Chemother 65:1359-67. 2010
    ..Here, other mutations in the embCAB operon are suggested to be involved in resistance development...
  61. doi Molecular characterization of secretory proteins Rv3619c and Rv3620c from Mycobacterium tuberculosis H37Rv
    Anjum Mahmood
    Molecular and Structural Biology Division, Central Drug Research Institute, Lucknow, India
    FEBS J 278:341-53. 2011
    Rv3619c and Rv3620c are the secretory, antigenic proteins of the ESAT-6/CFP-10 family of Mycobacterium tuberculosis H37Rv. In this article, we show that Rv3619c interacts with Rv3620c to form a 1 : 1 heterodimeric complex with a ..
  62. doi PknB-mediated phosphorylation of a novel substrate, N-acetylglucosamine-1-phosphate uridyltransferase, modulates its acetyltransferase activity
    Amit Parikh
    National Institute of Immunology, New Delhi, India
    J Mol Biol 386:451-64. 2009
    ..These findings imply a role for PknB in regulating peptidoglycan synthesis by modulating the acetyltransferase activity of GlmU...
  63. doi Temperature stability of proteins essential for the intracellular survival of Mycobacterium tuberculosis
    Nathan A Lack
    Department of Pharmacology, University of Oxford, Mansfield Road, Oxford OX13QT, UK
    Biochem J 418:369-78. 2009
    ..As propionyl-CoA is a product of cholesterol catabolism, we propose that NAT could have a role in the utilization of this important cofactor...
  64. ncbi inhA, a gene encoding a target for isoniazid and ethionamide in Mycobacterium tuberculosis
    A Banerjee
    Howard Hughes Medical Institute, Albert Einstein College of Medicine, Bronx, NY 10461
    Science 263:227-30. 1994
    ..These results suggest that InhA is likely a primary target of action for INH and ETH...
  65. pmc The salicylate-derived mycobactin siderophores of Mycobacterium tuberculosis are essential for growth in macrophages
    J J De Voss
    Department of Chemistry, University of Queensland, Brisbane, Queensland, Australia 4067
    Proc Natl Acad Sci U S A 97:1252-7. 2000
    ..tuberculosis. These results provide conclusive evidence linking this genetic locus to siderophore production...
  66. ncbi Crystal structure of the transcription elongation/anti-termination factor NusA from Mycobacterium tuberculosis at 1.7 A resolution
    B Gopal
    Division of Protein Structure, National Institute for Medical Research, Mill Hill, London, UK
    J Mol Biol 314:1087-95. 2001
    ..They also allow us to rationalize certain termination-defective and cold shock-sensitive mutations in the nusA gene that have been studied in Escherichia coli...
  67. ncbi Structure of Mycobacterium tuberculosis PknB supports a universal activation mechanism for Ser/Thr protein kinases
    Tracy A Young
    Department of Molecular and Cell Biology, 229 Stanley Hall 3206, University of California, Berkeley, California 94720 3206, USA
    Nat Struct Biol 10:168-74. 2003
    ..The structural and chemical similarities of PknB to metazoan homologs support a universal activation mechanism of Ser/Thr protein kinases in prokaryotes and eukaryotes...
  68. pmc Structure and function of the complex formed by the tuberculosis virulence factors CFP-10 and ESAT-6
    Philip S Renshaw
    Department of Biochemistry, University of Leicester, Leicester, UK
    EMBO J 24:2491-8. 2005
    ....
  69. doi Polyphosphate kinase 2: a modulator of nucleoside diphosphate kinase activity in mycobacteria
    Kamakshi Sureka
    Department of Chemistry, Bose Institute, 93 1 Acharya Prafulla Chandra Road, Kolkata 700009, India
    Mol Microbiol 74:1187-97. 2009
    ..Downregulation of ppk2 impairs survival of M. tuberculosis in macrophages, suggesting that PPK2 plays an important role in the physiology of the bacteria residing within macrophages...
  70. pmc CtpV: a putative copper exporter required for full virulence of Mycobacterium tuberculosis
    Sarah K Ward
    Department of Pathobiological Sciences, University of Wisconsin Madison, 1656 Linden Drive, Madison, WI 53706, USA
    Mol Microbiol 77:1096-110. 2010
    ..tuberculosis, supporting the hypothesis that copper response could be important to intracellular pathogens, in general...
  71. ncbi Crystal structure of shikimate kinase from Mycobacterium tuberculosis reveals the dynamic role of the LID domain in catalysis
    Yijun Gu
    Macromolecular Crystallography Laboratory, National Cancer Institute, Frederick, MD 21702, USA
    J Mol Biol 319:779-89. 2002
    ..The crystal structure of MtSK (this work) and that of Erwinia chrysanthemi SK suggest a concerted conformational change of the LID and SB domains upon nucleotide binding...
  72. ncbi Protein kinase G from pathogenic mycobacteria promotes survival within macrophages
    Anne Walburger
    Biozentrum, University of Basel, Klingelbergstr 50 70, CH 4056 Basel, Switzerland
    Science 304:1800-4. 2004
    ....
  73. pmc Cooperative binding of phosphorylated DevR to upstream sites is necessary and sufficient for activation of the Rv3134c-devRS operon in Mycobacterium tuberculosis: implication in the induction of DevR target genes
    Santosh Chauhan
    Department of Biotechnology, All India Institute of Medical Sciences, New Delhi, India
    J Bacteriol 190:4301-12. 2008
    ..The Rv3134c and hspX promoters have a similar architecture, wherein the proximal DevR-P binding site overlaps with the promoter -35 element. A model for the likely mode of action of DevR at these promoters is discussed...
  74. pmc Interaction of DevR with multiple binding sites synergistically activates divergent transcription of narK2-Rv1738 genes in Mycobacterium tuberculosis
    Santosh Chauhan
    Department of Biotechnology, All India Institute of Medical Sciences, New Delhi 110029, India
    J Bacteriol 190:5394-403. 2008
    ..tuberculosis. Analysis of narK2 promoter activity indicates that it is under negative regulation in addition to DevR-mediated positive regulation and also reveals differences between M. tuberculosis and Mycobacterium bovis BCG...
  75. pmc Biosynthesis of mycobacterial arabinogalactan: identification of a novel alpha(1-->3) arabinofuranosyltransferase
    Helen L Birch
    School of Biosciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK
    Mol Microbiol 69:1191-206. 2008
    ..This newly discovered glycosyltransferase sheds further light on the complexities of Mycobacterium cell wall biosynthesis, such as in M. tuberculosis and related species and represents a potential new drug target...
  76. ncbi Protein kinase E of Mycobacterium tuberculosis has a role in the nitric oxide stress response and apoptosis in a human macrophage model of infection
    Deepak Jayakumar
    Department of Immunology, Tuberculosis Research Centre ICMR, Chetput, Chennai 600031, India
    Cell Microbiol 10:365-74. 2008
    ..These findings suggest a novel mechanism, by which PknE senses nitric oxide stress and prevents apoptosis by interfering with host signalling pathways...
  77. pmc A novel lipase belonging to the hormone-sensitive lipase family induced under starvation to utilize stored triacylglycerol in Mycobacterium tuberculosis
    Chirajyoti Deb
    Burnett College of Biomedical Sciences, University of Central Florida, Orlando, 32816, USA
    J Biol Chem 281:3866-75. 2006
    Twenty-four putative lipase/esterase genes of Mycobacterium tuberculosis H37Rv were expressed in Escherichia coli and assayed for long-chain triacylglycerol (TG) hydrolase activity...
  78. ncbi Mycobacterium tuberculosis is a natural mutant with an inactivated oxidative-stress regulatory gene: implications for sensitivity to isoniazid
    V Deretic
    Department of Microbiology, University of Texas Health Science Center at San Antonio 78284 7758, USA
    Mol Microbiol 17:889-900. 1995
    ..tuberculosis is paradoxical considering the fact that survival in host macrophages is regarded as a critical feature of this pathogen, it offers a partial explanation for the exquisite sensitivity of M. tuberculosis to isoniazid...
  79. ncbi Identification of novel intergenic repetitive units in a mycobacterial two-component system operon
    P Supply
    Laboratoire de Microbiologie Génétique et Moléculaire, INSERM U447, Institut Pasteur de Lille, France
    Mol Microbiol 26:991-1003. 1997
    ..Analyses of the sequences at the insertion sites suggest that MIRUs disseminate by transposition into DTGA sites involved in translational coupling in polycistronic operons...
  80. ncbi The proteasome of Mycobacterium tuberculosis is required for resistance to nitric oxide
    K Heran Darwin
    Department of Microbiology and Immunology, Weill Medical College of Cornell University, New York, NY 10021, USA
    Science 302:1963-6. 2003
    ..Thus, the mycobacterial proteasome serves as a defense against oxidative or nitrosative stress...
  81. ncbi CFP10 discriminates between nonacetylated and acetylated ESAT-6 of Mycobacterium tuberculosis by differential interaction
    Limei Meng Okkels
    Department of Infectious Disease Immunology, Statens Serum Institut, Copenhagen, Denmark
    Proteomics 4:2954-60. 2004
    ..This example shows that the access to the protein species level can be a prerequisite to understand regulation of protein-protein interaction...
  82. ncbi The Mycobacterium tuberculosis PhoPR two-component system regulates genes essential for virulence and complex lipid biosynthesis
    Shaun B Walters
    TB Center, The Public Health Research Institute, 225 Warren Street, Newark, NJ 07103, USA
    Mol Microbiol 60:312-30. 2006
    ....
  83. ncbi C-terminal signal sequence promotes virulence factor secretion in Mycobacterium tuberculosis
    Patricia A Digiuseppe Champion
    Department of Microbiology and Immunology, University of California, San Francisco, 600 16th Street, Campus Box 2200, San Francisco, CA 94143 2200, USA
    Science 313:1632-6. 2006
    ..Attachment of the signal to yeast ubiquitin was sufficient for secretion from M. tuberculosis cells, demonstrating that this ESX-1 signal is portable...
  84. pmc Role of the dosR-dosS two-component regulatory system in Mycobacterium tuberculosis virulence in three animal models
    Paul J Converse
    Center for Tuberculosis Research, Johns Hopkins University School of Medicine, Baltimore, Maryland 21231, USA
    Infect Immun 77:1230-7. 2009
    ..Our analyses reveal that the dosR and dosS genes are required for full virulence and that there may be differences in the patterns of attenuation of this mutant between the animal models studied...
  85. doi Expression and characterization of two functional methionine aminopeptidases from Mycobacterium tuberculosis H37Rv
    Xuelian Zhang
    State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Handan Road 220, Shanghai, 200433, China
    Curr Microbiol 59:520-5. 2009
    ..the two MetAPs in Mycobacterium tuberculosis, Rv0734 and Rv2861c genes encoding MetAPs from genome of Mycobacterium tuberculosis H37Rv were cloned and expressed in Escherichia coli...
  86. doi Phosphorylation of InhA inhibits mycolic acid biosynthesis and growth of Mycobacterium tuberculosis
    Virginie Molle
    Institut de Biologie et Chimie des Proteines, Université Lyon1, IFR128 BioSciences, Lyon Gerland, Lyon Cedex 07, France
    Mol Microbiol 78:1591-605. 2010
    ..This study suggests that phosphorylation of InhA may represent an unusual mechanism that allows M. tuberculosis to regulate its mycolic acid content, thus offering a new approach to future anti-tuberculosis drug development...
  87. pmc Mycobacterium tuberculosis eis regulates autophagy, inflammation, and cell death through redox-dependent signaling
    Dong Min Shin
    Department of Microbiology, College of Medicine, Chungnam National University, Daejeon, Korea
    PLoS Pathog 6:e1001230. 2010
    ..Collectively, these data indicate that Mtb Eis suppresses host innate immune defenses by modulating autophagy, inflammation, and cell death in a redox-dependent manner...
  88. ncbi Execution of macrophage apoptosis by PE_PGRS33 of Mycobacterium tuberculosis is mediated by Toll-like receptor 2-dependent release of tumor necrosis factor-alpha
    Sanchita Basu
    Department of Chemistry, Bose Institute, 93 1 Acharya Prafulla Chandra Road, Kolkata 700009, India
    J Biol Chem 282:1039-50. 2007
    ..These results provide the first evidence that variations in the polymorphic repeats of the PGRS domain modulate the innate immune response...
  89. ncbi Cloning, expression, purification and characterization of DNA topoisomerase I of Mycobacterium tuberculosis
    F Yang
    Department of Medicine, University of Pennsylvania, Philadelphia 19104, USA
    Gene 178:63-9. 1996
    ..Unlike the more well-characterized E. coli Topo I, MTb Topo I does not contain a zinc-finger DNA-binding motif in the C-terminal domain of the protein...
  90. ncbi Structural insights into catalysis and inhibition of O-acetylserine sulfhydrylase from Mycobacterium tuberculosis. Crystal structures of the enzyme alpha-aminoacrylate intermediate and an enzyme-inhibitor complex
    Robert Schnell
    Department of Medical Biochemistry and Biophysics, Karolinska Institutet, S 171 77 Stockholm, Sweden
    J Biol Chem 282:23473-81. 2007
    ..The structure of the enzyme-peptide complex provides a framework for the design of strong binding inhibitors...
  91. pmc Ligand specificity of group I biotin protein ligase of Mycobacterium tuberculosis
    Sudha Purushothaman
    Molecular Biophysics Unit, Indian Institute of Science, Bangalore, India
    PLoS ONE 3:e2320. 2008
    ..Biotin carboxyl carrier protein (BCCP) provides the co-factor for catalytic activity of ACC...
  92. pmc Cloning and expression of Mycobacterium tuberculosis and Mycobacterium leprae dihydropteroate synthase in Escherichia coli
    V Nopponpunth
    Department of Biochemistry, Faculty of Science, Mahidol University, Bangkok 10400, Thailand
    J Bacteriol 181:6814-21. 1999
    ....
  93. ncbi Adenylyl cyclase Rv0386 from Mycobacterium tuberculosis H37Rv uses a novel mode for substrate selection
    Lucila I Castro
    Abteilung Pharmazeutische Biochemie, Fakultät für Chemie und Pharmazie, Universitat Tubingen, Germany
    FEBS J 272:3085-92. 2005
    ..Data from individual and coordinated point mutations suggest a model for purine definition based on an amide switch related to that previously identified in cyclic nucleotide phosphodiesterases...
  94. ncbi NAD+-dependent DNA ligase (Rv3014c) from Mycobacterium tuberculosis: novel structure-function relationship and identification of a specific inhibitor
    Sandeep Kumar Srivastava
    Molecular and Structural Biology Division, Central Drug Research Institute, Lucknow 226001, Uttar Pradesh, India
    Proteins 69:97-111. 2007
    ..An analysis of conserved water in the binding site of the enzyme suggests strategies for synthesis of improved inhibitors with better specificity and potency...
  95. doi Dimerisation and structural integrity of Heparin Binding Hemagglutinin A from Mycobacterium tuberculosis: implications for bacterial agglutination
    Carla Esposito
    Istitute of Biostructures and Bioimaging, CNR, Naples, Italy
    FEBS Lett 584:1091-6. 2010
    ..Our data suggest that agglutination-driven cell-cell interactions do not occur via association of HBHA monomers, nor via association of HBHA dimers and open the scenario to a possible trans-dimerisation process...
  96. doi Crystal structure of DNA polymerase III β sliding clamp from Mycobacterium tuberculosis
    Wen Jun Gui
    National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Chaoyang District, Beijing 100101, China
    Biochem Biophys Res Commun 405:272-7. 2011
    ..tuberculosis sliding clamp and peptides derived from the α and δ subunits of Pol III, which indicated that the LF motif also plays an important role in the binding of the α and δ subunits to the sliding clamp of M. tuberculosis...
  97. doi Downregulation of katG expression is associated with isoniazid resistance in Mycobacterium tuberculosis
    Hiroki Ando
    National Center for Global Health and Medicine, 1 21 1 Toyama, Shinjuku, Tokyo 162 8655, Japan
    Mol Microbiol 79:1615-28. 2011
    ..These results suggested that downregulation of katG is a mechanism of INH resistance in M. tuberculosis and that mutations in the furA-katG intergenic region play a role in this resistance mechanism...
  98. doi Studies on structural and functional divergence among seven WhiB proteins of Mycobacterium tuberculosis H37Rv
    Md Suhail Alam
    Institute of Microbial Technology, CSIR, Chandigarh, India
    FEBS J 276:76-93. 2009
    ..The structural and functional divergence among WhiB proteins indicated that each WhiB protein is a distinguished member of the same family and together they may represent a novel redox system for M. tuberculosis...
  99. pmc Cloning and B-cell-epitope mapping of MPT64 from Mycobacterium tuberculosis H37Rv
    T Oettinger
    Mycobacteria Department, Statens Seruminstitut, Copenhagen, Denmark
    Infect Immun 62:2058-64. 1994
    The gene of the immunogenic protein MPT64 found in culture filtrates of Mycobacterium tuberculosis H37Rv was cloned and sequenced...
  100. ncbi A new subfamily of short bacterial adenylate kinases with the Mycobacterium tuberculosis enzyme as a model: A predictive and experimental study
    H Munier-Lehmann
    Laboratoire de Chimie Structurale des Macromolecules, Institut Pasteur, France
    Proteins 36:238-48. 1999
    ..coli and on secondary structure predictions for various sequenced AKs, we propose a structural model for AK from M. tuberculosis (AKmt). Proteins 1999;36:238-248...
  101. ncbi Structural and dynamic studies on ligand-free adenylate kinase from Mycobacterium tuberculosis revealed a closed conformation that can be related to the reduced catalytic activity
    Simona Miron
    Institut National de la Santé et de la Recherche Médicale U350 et Institut Curie Recherche, Centre Universitaire, Batiments 110 112, F 91405 Orsay, France
    Biochemistry 43:67-77. 2004
    ..These structural and dynamic features of AKmt may be related to its low catalytic activity that is 10-fold lower than in their eukaryote counterparts...

Research Grants17

  1. Screen for Mycobacterium Tuberculosis (RMI)
    E White; Fiscal Year: 2005
    ..tuberculosis. 3) To study the effect of these novel inhibitors on the pantothenate pathway in M. smegmatis. 4) To co-crystallize these compounds with pantothenate synthetase to study their mode of binding ..
  2. Regulation of Cellular division in M. tuberculosis
    RICHARD SLAYDEN; Fiscal Year: 2006
    ..Thus, the studies proposed in this application are designed to examine the replication dynamics of MTB, specifically focusing on cell cycle-regulated genes that are involved in cell division. [unreadable] [unreadable] [unreadable]..
  3. MYCOBACTERIAL ENVIRONMENT WITHIN MACROPHAGES
    Luiz Bermudez; Fiscal Year: 2003
    ..We believe that the proposed studies will generate important information about the phagosome environment and the mycobacterial response to it. ..
  4. Post Translation Regulation in Mycobacteria
    Luiz Bermudez; Fiscal Year: 2008
    ..In case we confirm our hypothesis, future work will address the role of Ion in M. tuberculosis [unreadable] [unreadable] [unreadable]..
  5. Reactivation Tuberculosis in A/J
    Chinnaswamy Jagannath; Fiscal Year: 2005
    ..These studies are anticipated to enhance our understanding on putative mechanisms that precede the reactivation of tuberculosis in the lungs of mice and ultimately help us to develop better strategies to prevent tuberculosis in man. ..
  6. Mtb Proteasome-Associated Factors and Virulence
    KATERINA DARWIN; Fiscal Year: 2007
    ..Taken together it will be important to develop faster acting drugs to new targets in Mtb in order to better treat tuberculosis in the future. ..
  7. Structural Studies of AIDS-Responsive Drugs
    Vivian Cody; Fiscal Year: 2010
    ..These results will help guide the design of species selective inhibitors. Mutagenesis studies will be carried out to test these possibilitiesin the structure-based correlations to help design novel pjDHFRinhibitors. ..
  8. Genetic screens to identify mycobacterial porins
    Martin Pavelka; Fiscal Year: 2008
    ..This would be an important contribution to reduce the public health burden of mycobacterial disease in the United States. [unreadable] [unreadable] [unreadable]..
  9. Identification of secreted proteins important for tularemia pathogenesis
    Martin Pavelka; Fiscal Year: 2007
    ..Because of F. tularensis is a select agent and could be used a biological weapon, this research could potentially have a significant impact on public health in the future. [unreadable] [unreadable] [unreadable]..
  10. Identification of genes controlling TB in murine lungs
    Alexander Apt; Fiscal Year: 2005
    ....
  11. Structure Function Relationship in Hemeproteins
    Syun Ru Yeh; Fiscal Year: 2005
    ..Experiments are proposed to test the functional consequences of this finding. These hemeprotein systems provide an excellent model for investigating fundamental structural properties that underlie biological reactivity. ..
  12. Development of genetic tools for Francisella tularensis
    Martin Pavelka; Fiscal Year: 2004
    ..The further development of Francisella genetics is timely, as the sequencing of the genome of the F. tularensis subsp. tularensis, strain Schu 4 is almost complete. ..
  13. BIOSYNTHESIS OF THE MYCOBACTERIAL PEPTIDOGLYCAN
    Martin Pavelka; Fiscal Year: 2004
    ..For the aims of this proposal, the Pi will study M. tuberculosis and M. smegmatis as a model organism using the techniques of classical bacterial genetics, molecular biology and biochemistry. ..
  14. MtrA and Mycobacterium tuberculosis proliferation
    Malini Rajagopalan; Fiscal Year: 2007
    ..These experiments will establish whether oriC replication is a target of MtrA system and will improve our understanding of regulation of Mtb multiplication in vivo. [unreadable] [unreadable]..
  15. Congugal DNA transfer into M. tuberculosis
    KEITH DERBYSHIRE; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  16. MOLECULAR ANALYSIS OF THE INSERTION ELEMENT IS903
    KEITH DERBYSHIRE; Fiscal Year: 2004
    ..3. To establish biochemical assays that will identify specific transposase-DNA contacts, define stable domains of the protein and facilitate the development of an in vitro transposition system. ..
  17. CONJUGATION AND RECOMBINATION IN MYCOBACTERIA
    KEITH DERBYSHIRE; Fiscal Year: 2009
    ..2. To identify and characterize trans-acting transfer functions in both donor and recipient cells. 3. To examine transfer of chromosomal and plasmid DNA among fast- and slow-growing mycobacteria. ..

Patents25

  1. ENZYMATIC SYSTEM-CONTAINING COSMETIC COMPOSITIONS
    Patent Number: KR1020160044437-A; Date:2016-04-25
  2. ENZYMATIC SYSTEM-CONTAINING COSMETIC COMPOSITIONS
    Patent Number: JP2016520121-A; Date:2016-07-11
  3. MYCOBACTERIUM ANTIGENIC COMPOSITION
    Patent Number: KR1020140029376-A; Date:2014-03-10
  4. DIAGNOSIS AND TREATMENT OF MULTIPLE SULFATASE DEFICIENCY AND OTHER SULFATASE DEFICIENCIES
    Patent Number: JP2014131515-A; Date:2014-07-17
  5. Diagnosis and treatment of multiple sulfatase deficiency and other using a formylglycine generating enzyme (fge)
    Patent Number: WO2004072275-A2; Date:2004-08-26
  6. Highly conserved genes and their use to generate probes and primers for detection of microorganisms
    Patent Number: WO0123604-A2; Date:2001-04-05
  7. Proteins associated with abiotic stress response and homologs
    Patent Number: WO2007110314-A; Date:2007-10-04
  8. Reaction or detection tubular container, and reagent kit comprising said container
    Patent Number: JP2008200028-A; Date:2008-09-04
  9. COMPUTATIONAL METHOD FOR IDETIFYING ADHESIN
    Patent Number: JP2007520718-A; Date:2007-07-26
  10. RECOMBINANT HOST CELLS COMPRISING PHOSPHOKETOLASES
    Patent Number: KR1020130117753-A; Date:2013-10-28
  11. Fusion enzyme between Z,E-farnesyl diphosphate synthase and farnesyl diphosphate synthase from E.coli and use thereof
    Patent Number: KR1020130050178-A; Date:2013-05-15
  12. DIAGNOSIS AND TREATMENT OF MULTIPLE SULFATASE DEFICIENCY AND OTHER SULFATASE DEFICIENCIES
    Patent Number: JP2006517412-A; Date:2006-07-27
  13. MYCOBACTERIUM ANTIGENIC COMPOSITION
    Patent Number: WO2012080370-A1; Date:2012-06-21
  14. Nucleic acid fragments and polypeptide fragments derived from M. Tuberculosis
    Patent Number: EP1484405-A1; Date:2004-12-08
  15. Plants with increased yield
    Patent Number: WO2009037329-A2; Date:2009-03-26
  16. Nucleic acid fragments and polypeptide fragments derived from M. Tuberculosis
    Patent Number: EP1449922-A2; Date:2004-08-25
  17. DIAGNOSIS AND TREATMENT OF MULTIPLE SULFATASE DEFICIENCY AND OTHER SULFATASE DEFICIENCIES
    Patent Number: JP2012090630-A; Date:2012-05-17
  18. HIGHLY CONSERVED GENES AND THEIR USE TO GENERATE SPECIES-SPECIFIC, GENUS-SPECIFIC, FAMILY-SPECIFIC, GROUP-SPECIFIC AND UNIVERSAL NUCLEIC ACID PROBES AND AMPLIFICATION PRIMERS TO RAPIDLY DETECT AND IDENTIFY ALGAL, ARCHAEAL, BACTERIAL, FUNGAL AND PARASITICAL MICROOR-GANISMS FROM CLINICAL SPECIMENS FOR DIAGNOSIS
    Patent Number: JP2003511015-A; Date:2003-03-25
  19. DIAGNOSTIC SKIN TEST FOR TUBERCULOSIS
    Patent Number: WO9501440-A1; Date:1995-01-12
  20. AGENT EXHIBITING PROPERTIES TO FORM THE CELLULAR IMMUNITY AGAINST MYCOBACTERIUM TUBERCULOSIS H37 Rv, METHOD FOR THE PRODUCTION THEREOF (VARIANTS), A RECOMBINANT STRAIN AND AN AGENT FOR TUBERCULOSIS DIAGNOSIS
    Patent Number: EP2156844-A1; Date:2010-02-24
  21. Construction of a comparative database and identificaiton of virulence factors through comparison of polymorphic regions in clinical isolates of infectious organisms
    Patent Number: EP1789577-A2; Date:2007-05-30
  22. Proteins associated with abiotic stress response and homologs
    Patent Number: EP2221382-A2; Date:2010-08-25
  23. Process for the control of production of fine chemicals
    Patent Number: EP2199304-A1; Date:2010-06-23
  24. Process for the production of lutein
    Patent Number: EP2096177-A2; Date:2009-09-02
  25. Plants with increased yield and/or increased tolerance to environmental stress (iy-bm)
    Patent Number: WO2009077611-A2; Date:2009-06-25