Genomes and Genes
Mycobacterium sp. MCS
- Structural studies on MtRecA-nucleotide complexes: insights into DNA and nucleotide binding and the structural signature of NTP recognitionS Datta
Molecular Biophysics Unit, Indian Institute of Science, Bangalore, India
Proteins 50:474-85. 2003..Comparison with other NTP binding proteins reveals many commonalties in modes of binding by diverse members in the structural family, contributing to our understanding of the structural signature of NTP recognition...
- Crystal structures of Mycobacterium smegmatis RecA and its nucleotide complexesS Datta
Molecular Biophysics Unit, Indian Institute of Science, Bangalore 560 012, India
J Bacteriol 185:4280-4. 2003..These observations provide a framework for exploring the known properties of the RecA proteins...
- Crystal structures of Mycobacterium tuberculosis RecA and its complex with ADP-AlF(4): implications for decreased ATPase activity and molecular aggregationS Datta
Molecular Biophysics Unit, Department of Biochemistry and Bioinformatics Centre, Indian Institute of Science, Bangalore 560 012, India
Nucleic Acids Res 28:4964-73. 2000..The formation of a helical filament with a deep groove appears to be an inherent property of MtRecA. The histidine in loop L1 appears to be positioned appropriately for DNA interaction...
- Effect of Principal Component Analysis Centering and Scaling on Classification of Mycobacteria from Raman SpectraCynthia Hanson
Department of Biological Engineering, Utah State University, UT, USA
Appl Spectrosc . 2016..Centered and unscaled data provided the best results when selecting PCs based on cumulative percent variance...
- Comparative Genomics and Proteomic Analysis of Four Non-tuberculous Mycobacterium Species and Mycobacterium tuberculosis Complex: Occurrence of Shared Immunogenic ProteinsNomakorinte Gcebe
Tuberculosis Laboratory, Agricultural Research Council Onderstepoort Veterinary InstituteOnderstepoort, South Africa Department of Veterinary Tropical Diseases, Faculty of Veterinary Science, University of PretoriaOnderstepoort, South Africa
Front Microbiol 7:795. 2016..Identification of NTM homologs of immunogenic proteins warrants further investigation of their ability to cause cross-reactive immune responses with MTBC antigens. ..
- Mycobacterium smegmatis ferredoxin: a unique distribution of cysteine residues constructing iron--sulfur clustersT Hase
FEBS Lett 103:224-8. 1979
- The solution structure of acyl carrier protein from Mycobacterium tuberculosisHing C Wong
Department of Structural Biology, St Jude Children s Research Hospital, Memphis, Tennessee 38105, USA
J Biol Chem 277:15874-80. 2002....
- Structure of Mycobacterium tuberculosis PknB supports a universal activation mechanism for Ser/Thr protein kinasesTracy A Young
Department of Molecular and Cell Biology, 229 Stanley Hall 3206, University of California, Berkeley, California 94720 3206, USA
Nat Struct Biol 10:168-74. 2003..The structural and chemical similarities of PknB to metazoan homologs support a universal activation mechanism of Ser/Thr protein kinases in prokaryotes and eukaryotes...
- Crystal structure of the catalytic domain of the PknB serine/threonine kinase from Mycobacterium tuberculosisMiguel Ortiz-Lombardia
Unité de Biochimie Structurale, URA 2185 CNRS, Institut Pasteur, 25, rue du Dr Roux, 75724 Paris, Cedex 15, France
J Biol Chem 278:13094-100. 2003..It presents as well a partially disordered activation loop, suggesting an induced fit mode of binding for the so far unknown substrates of this kinase or for some modulating factor(s)...
- Identification of pyrene-induced proteins in Mycobacterium sp. strain 6PY1: evidence for two ring-hydroxylating dioxygenasesSerge Krivobok
Laboratoire de Biochimie et Biophysique des Systemes Integres, CNRS UMR 5092, CEA Grenoble, F 38054 Grenoble Cedex 9, France
J Bacteriol 185:3828-41. 2003..In contrast, Pdo2 could be detected only in PAH-grown cells. These results indicated that the two enzymes were differentially regulated depending on the carbon source used for growth...
- Mycobacterium tuberculosis chaperonin 10 heptamers self-associate through their biologically active loopsMichael M Roberts
Medical Microbiology, Department of Cellular and Molecular Medicine, St George s Hospital Medical School, London SW17 0RE, England
J Bacteriol 185:4172-85. 2003..Electron density corresponding to a partially disordered protein subunit appears encapsulated within the interior dome cavity of each heptamer. This suggests that the binding of substrates to cpn10 is possible in the absence of cpn60...
- Identification of a regulated alkaline phosphatase, a cell surface-associated lipoprotein, in Mycobacterium smegmatisJordan Kriakov
Department of Microbiology and Immunology, Howard Hughes Medical Institute, Albert Einstein College of Medicine, Bronx, New York 10461, USA
J Bacteriol 185:4983-91. 2003..Furthermore, immunoprecipitation of PhoA from [(14)C]acetate-labeled M. smegmatis cell lysates demonstrated that this phosphatase is a lipoprotein...
- rpoB-based identification of nonpigmented and late-pigmenting rapidly growing mycobacteriaToidi Adekambi
Unite des Rickettsies, CNRS UMR 6020 IFR 48, Faculte de Medecine, Universite de la Mediterranee, Marseille, France
J Clin Microbiol 41:5699-708. 2003..We conclude that molecular identification by analysis of the 723-bp rpoB sequence is a rapid and accurate tool for identification of RGM...
- Isolation and characterization of polycyclic aromatic hydrocarbon-degrading Mycobacterium isolates from soilC D Miller
Department of Biology, Utah State University, Logan, UT 84322, USA
Microb Ecol 48:230-8. 2004..We find that dioxygenase genes nidA and nidB are present in each of the Libby Mycobacterium isolates and are adjacent to each other in the sequence nidB-nidA, an order that is unique to the PAH-degrading mycobacteria...
- Crystal structure of the biologically active form of class Ib ribonucleotide reductase small subunit from Mycobacterium tuberculosisMalin Uppsten
Department of Molecular Biology, Swedish University of Agricultural Sciences, Uppsala Biomedical Center, P O Box 590, SE 751 24 Uppsala, Sweden
FEBS Lett 569:117-22. 2004..In the R2F-2 structure, we also could see a mobility of alphaE in between the two conformations...
- An alternate conformation and a third metal in PstP/Ppp, the M. tuberculosis PP2C-Family Ser/Thr protein phosphataseKristi E Pullen
Department of Molecular and Cell Biology, 339 Hildebrand Hall 3206, University of California, Berkeley, Berkeley, CA 94720, USA
Structure 12:1947-54. 2004..The PstP/Ppp structure reveals core functional motifs that advance the framework for understanding the mechanisms of substrate recognition, catalysis, and regulation of PP2C phosphatases...
- Dissection of phylogenetic relationships among 19 rapidly growing Mycobacterium species by 16S rRNA, hsp65, sodA, recA and rpoB gene sequencingToidi Adekambi
Unite des Rickettsies, CNRS UMR 6020 IFR 48, Faculte de Medecine, Universite de la Mediterranee, 27, Boulevard Jean Moulin, 13385 Marseille Cedex 05, France
Int J Syst Evol Microbiol 54:2095-105. 2004..This was in agreement with phenotypic and biochemical characteristics and suggested that these strains are representatives of two closely related, albeit distinct species...
- Amoebal coculture of "Mycobacterium massiliense" sp. nov. from the sputum of a patient with hemoptoic pneumoniaToidi Adekambi
Unite des Rickettsies, CNRS UMR 6020 IFR 48, Faculte de Medecine, Université de la Méditteranée, Marseille, France
J Clin Microbiol 42:5493-501. 2004..The type strain has been deposited in the Collection Institut Pasteur as CIP 108297(T) and in Culture Collection of the University of Goteborg, Goteborg, Sweden, as CCUG 48898(T)...
- rpoB gene sequence-based characterization of emerging non-tuberculous mycobacteria with descriptions of Mycobacterium bolletii sp. nov., Mycobacterium phocaicum sp. nov. and Mycobacterium aubagnense sp. novToidi Adekambi
Unite des Rickettsies, CNRS UMR 6020 IFR 48, Faculte de Medecine, 27, Boulevard Jean Moulin, Université de la Méditerranée and Assistance Publique Hôpitaux de Marseille Timone, Fédération de Microbiologie Clinique, Marseille, France
Int J Syst Evol Microbiol 56:133-43. 2006....
- Molecular cloning and expression of genes encoding a novel dioxygenase involved in low- and high-molecular-weight polycyclic aromatic hydrocarbon degradation in Mycobacterium vanbaalenii PYR-1Seong Jae Kim
Division of Microbiology, National Center for Toxicological Research U S FDA, Jefferson, Arkansas 72079, USA
Appl Environ Microbiol 72:1045-54. 2006..The recombinant E. coli, however, did not show any dioxygenation activity for phthalate and biphenyl. The upregulation of nidA3B3 in M. vanbaalenii PYR-1 induced by PAHs was confirmed by reverse transcription-PCR analysis...
- Cloning and characterization of CYP51 from Mycobacterium aviumMichael P Pietila
Division of Pulmonary and Critical Care Medicine, Thoracic Diseases Research Unit, 826 Stabile Building, Mayo Clinic College of Medicine, Rochester, MN 55905, USA
Am J Respir Cell Mol Biol 35:236-42. 2006..Itraconazole, voriconazole, and fluconazole did not inhibit MAC growth to any significant extent...
- Crystallographic identification of an ordered C-terminal domain and a second nucleotide-binding site in RecA: new insights into allosteryR Krishna
Molecular Biophysics Unit, Indian Institute of Science, Bangalore 560 012, India
Nucleic Acids Res 34:2186-95. 2006....
- Genetic basis for natural and acquired resistance to the diarylquinoline R207910 in mycobacteriaStephanie Petrella
Laboratoire de Recherche Moleculaire sur les Antibiotiques, LRMA INSERM U655, Universite Pierre et Marie Curie, F 75634 Paris Cedex 13, France
Antimicrob Agents Chemother 50:2853-6. 2006..xenopi to R207910...
- Isolation and sequence of a Mycobacterium tuberculosis sigma factorM L Beggs
Department of Pathology, University of Arkansas for Medical Sciences, Little Rock, USA
Gene 174:285-7. 1996..The protein encoded by this gene is 92% similar to the Mycobacterium smegmatis sigma factor MysB, and has been designated Mtu SigB. A Mycobacterium leprae homologue of mysB and mtu sigB was identified in the database...
- Sequence and immunogenicity of the 70-kDa heat shock protein of Mycobacterium lepraeK R McKenzie
Centenary Institute of Cancer Medicine and Cell Biology, University of Sydney, Australia
J Immunol 147:312-9. 1991..leprae recombinant proteins were lower than to mAb affinity-purified BCG p70. Thus, the M. leprae 70-kDa heat shock protein elicits T and B cell responses in subjects exposed to mycobacteria, despite its homology with the human hsp70...
- Characterization of RNA polymerase and two sigma-factor genes from Mycobacterium smegmatisM Predich
Department of Microbiology, Public Health Research Institute, New York, New York 10016, USA
Mol Microbiol 15:355-66. 1995..We also demonstrated in vitro transcription of the M. smegmatis rrnB promoter by the M. smegmatis RNA polymerase. Significantly, highly active B. subtilis RNA polymerase holoenzyme was unable to transcribe this gene...
- Cloning and sequence analysis of the rpsL and rpsG genes of Mycobacterium smegmatis and characterization of mutations causing resistance to streptomycinT J Kenney
Department of Microbiology and Immunology, Emory University, Atlanta, Georgia 30322
J Bacteriol 176:6153-6. 1994..Forty-six percent of the mutations (13 of 28) were due to a transition changing an AAG Lys codon to an AGG Arg codon, with eight changes at codon 43 and five at codon 88...
- Sequence and expression of the Mycobacterium leprae dnaJ geneS S Harvey
Centenary Institute of Cancer Medicine and Cell Biology, University of Sydney, NSW, Australia
J Gen Microbiol 139:2003-8. 1993..leprae and M. tuberculosis (89%) with significant divergence between the two occurring only at the C-terminal end. The expressed recombinant DnaJ protein had a molecular mass of 42 kDa...
- Sequence and analysis of the rpoB gene of Mycobacterium smegmatisS V Hetherington
Department of Infectious Diseases, St Jude Children s Research Hospital, Memphis, Tennessee, USA
Antimicrob Agents Chemother 39:2164-6. 1995..Mycobacterium smegmatis is naturally resistant to rifampin, but analysis of the rpoB gene revealed no identifiable rifampin resistance mutations. Another mechanism of resistance may be present...
- Mycobacterium smegmatis DNA gyrase: cloning and overexpression in Escherichia coliK Madhusudan
Centre for Genetic Engineering, Indian Institute of Science, Bangalore, India
Microbiology 141:3029-37. 1995..This activity was ATP-dependent and novobiocin-sensitive. The identity of the genes was also confirmed by complementation analysis...
- Functional characterization of the precursor and spliced forms of RecA protein of Mycobacterium tuberculosisR A Kumar
Department of Biochemistry, Indian Institute of Science, Bangalore, India
Biochemistry 35:1793-802. 1996..The probability of an association between the lack and partial levels of biological activity of RecA protein(s) to that of illegitimate recombination in pathogenic mycobacteria is considered...
- Organization of the origins of replication of the chromosomes of Mycobacterium smegmatis, Mycobacterium leprae and Mycobacterium tuberculosis and isolation of a functional origin from M. smegmatisL Salazar
Laboratorio de Genetica Molecular, Instituto Venezolano de Investigaciones Cientificas IVIC, Caracas, Venezuela
Mol Microbiol 20:283-93. 1996..smegmatis, containing the dnaA-dnaN region, was shown to promote autonomous replication in M. smegmatis, unlike the corresponding regions from M. tuberculosis or M. leprae...
- Sequence analysis, purification, and study of inhibition by 4-quinolones of the DNA gyrase from Mycobacterium smegmatisV Revel-Viravau
Laboratoire de Recherche Moleculaire sur les Antibiotiques, Universite Pierre et Marie Curie, Faculté de Médecine Pitié Salpêtrière, Paris, France
Antimicrob Agents Chemother 40:2054-61. 1996....
- Molecular cloning, nucleotide sequence, and expression of genes encoding a polycyclic aromatic ring dioxygenase from Mycobacterium sp. strain PYR-1A A Khan
Division of Microbiology, National Center for Toxicological Research, Food and Drug Administration, Jefferson, Arkansas 72079, USA
Appl Environ Microbiol 67:3577-85. 2001..The functionality of the genes for PAH degradation was confirmed in a phagemid clone containing all three genes, as well as in plasmid subclones containing the two genes encoding the dioxygenase subunits...
- Mycobacterial recA is cotranscribed with a potential regulatory gene called recXK G Papavinasasundaram
Division of Mycobacterial Research, National Institute for Medical Research, Mill Hill, London, UK
Mol Microbiol 24:141-53. 1997..Reverse transcription/polymerase chain reaction analysis of M. smegmatis RNA demonstrated that recA and orf3 (recX) are within the same transcriptional unit...
- Mycobacterium tuberculosis chaperonin 10 stimulates bone resorption: a potential contributory factor in Pott's diseaseS Meghji
Maxillofacial Surgery Research Unit, Eastman Dental Institute, University College London, United Kingdom
J Exp Med 186:1241-6. 1997..Our findings suggest that Mt cpn10 may be a valuable pharmacological target for the clinical therapy of vertebral tuberculosis and possibly other bone diseases...
- Identification of an ABC transporter gene that exhibits mRNA level overexpression in fluoroquinolone-resistant Mycobacterium smegmatisS K Banerjee
Institute of Microbial Technology, Chandigarh, India
FEBS Lett 425:151-6. 1998..Thus this is the first report in any prokaryote indicating differential expression of an ABC transporter in a fluoroquinolone resistant colony...
- Molecular analysis of the gene encoding F420-dependent glucose-6-phosphate dehydrogenase from Mycobacterium smegmatisE Purwantini
Department of Microbiology, University of Iowa, Iowa City 52242, USA
J Bacteriol 180:2212-9. 1998..Multiple alignment of FGD with the hypothetical proteins also revealed portions of the same conserved sequences. Moderately high levels of FGD were expressed in Escherichia coli BL21(DE3) carrying fgd in pBluescript...
- Construction and complementation of a recA deletion mutant of Mycobacterium smegmatis reveals that the intein in Mycobacterium tuberculosis recA does not affect RecA functionK G Papavinasasundaram
Division of Mycobacterial Research, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, UK
Mol Microbiol 30:525-34. 1998..tuberculosis recA gene contained or lacked the intein. Thus, the low frequency of homologous recombination observed in M. tuberculosis is not due to the presence of an intein-coding sequence in its recA gene per se...
- Crystallization, x-ray diffraction and preliminary structure analysis of Mycobacterium tuberculosis chaperonin 10M M Roberts
Department of Medical Microbiology, St George s Hospital Medical School, Cranmer Terrace, London SW17 0RE, England
Acta Crystallogr D Biol Crystallogr 55:910-4. 1999..The structure determination will permit the analysis of the amino acids identified as important for the protein-folding and cell-signalling activity of Mtcpn10...
- Evaluation of recA sequences for identification of Mycobacterium speciesK S Blackwood
National Reference Centre for Mycobacteriology, Bureau of Microbiology, Health Sciences Center, Canada
J Clin Microbiol 38:2846-52. 2000..tuberculosis and M. leprae. We propose that recA gene sequencing offers a complementary method to 16S rRNA gene sequencing for the accurate identification of the Mycobacterium species...
- A genetic mechanism for deletion of the ser2 gene cluster and formation of rough morphological variants of Mycobacterium aviumT M Eckstein
Mycobacteria Research Laboratories, Department of Microbiology, Colorado State University, Fort Collins, Colorado 80523 1677, USA
J Bacteriol 182:6177-82. 2000..and provides further evidence that homologous recombination between insertion sequence elements can be a primary determinant of genome plasticity in these bacteria...
- A purF mutant of Mycobacterium smegmatis has impaired survival during oxygen-starved stationary phaseJ Keer
Department of Biology, Imperial College of Science, Technology and Medicine, Imperial College Road, London SW7 2AZ, UK
Microbiology 147:473-81. 2001....
- A GTP-dependent vertebrate-type phosphoenolpyruvate carboxykinase from Mycobacterium smegmatisB Mukhopadhyay
Department of Microbiology, University of Illinois at Urbana Champaign, Urbana, Illinois 61801, USA
J Biol Chem 276:16137-45. 2001..Both in primary structure and kinetic properties, the mycobacterial PCK was very similar to its vertebrate-liver counterparts and thus could serve as a model for these enzymes; examples for several immediate targets are presented...
- Molecular characterization of a phenanthrene degradation pathway in Mycobacterium vanbaalenii PYR-1Robin L Stingley
National Center for Toxicological Research, US FDA, 3900 NCTR Road, Jefferson, AR 72079, USA
Biochem Biophys Res Commun 322:133-46. 2004..This study is the first to identify genes responsible for the degradation of phenanthrene via the phthalic acid pathway in Mycobacterium species...
- Mycobacterium tuberculosis GroEL homologues unusually exist as lower oligomers and retain the ability to suppress aggregation of substrate proteinsRohini Qamra
Laboratory of Structural Biology, Centre for DNA Fingerprinting and Diagnostics, ECIL Road, Nacharam, Hyderabad 500076, India
J Mol Biol 342:605-17. 2004..We hypothesize that the ATP independent chaperones might offer benefit to the pathogen by promoting its existence in the latent phase of its life cycle...
- Novel organization of genes in a phthalate degradation operon of Mycobacterium vanbaalenii PYR-1Robin L Stingley
Division of Microbiology, National Center for Toxicological Research, US Food and Drug Administration, Jefferson, AR 72079, USA
Microbiology 150:3749-61. 2004..In addition, the M. vanbaalenii PYR-1 pht operon contains no decarboxylase gene and none was identified within a 37 kb region containing the operon. This study is the first report of a phthalate degradation operon in Mycobacterium spp...
- Strain variation in Mycobacterium marinum fish isolatesM Ucko
Israel Oceanographic and Limnological Research Ltd, National Center for Mariculture, Eilat, Israel
Appl Environ Microbiol 68:5281-7. 2002....
- A polymorphic region in Mycobacterium abscessus contains a novel insertion sequence elementSusan T Howard
University of New Mexico School of Medicine, Dept of Internal Medicine, 915 Camino de Salud, Albuquerque 87131, USA
Microbiology 148:2987-96. 2002..avium and Mycobacterium tuberculosis, than to the rapid-grower M. smegmatis. Several ORFs also have strong similarity to Pseudomonas aeruginosa genes with a potential role in beta-oxidation...
- Cloning and expression in Escherichia coli of DNA encoding a 60 kDa stress protein of Mycobacterium paratuberculosis, the causative agent of Johne's diseaseA Colston
Department of Veterinary Pathology, University of Edinburgh, Summerhall, UK
Microbiology 140:3329-36. 1994..paratuberculosis 60 kDa stress protein was liberated from GST by proteolytic cleavage with either thrombin or Factor Xa enzyme. Authenticity of liberated recombinant stress protein was confirmed by N-terminal amino acid sequencing...
- Molecular and immunological characterization of Mycobacterium avium 65 kDa heat shock protein (Hsp65)V Nagabhushanam
Department of Microbiology and Immunology, University of Melbourne, Victoria, Australia
Immunol Cell Biol 79:454-61. 2001..Therefore, the findings indicate that Hsp65 is not a dominant T-cell antigen during M. avium infection...
- Plants with increased tolerance and/or resistance to environmental stress and increased biomass productionPatent Number: WO2008142034-A2; Date:2008-11-27
- Plants with increased yieldPatent Number: WO2009037279-A1; Date:2009-03-26
- Plants with increased yieldPatent Number: WO2009037329-A2; Date:2009-03-26
- Plants with increased yield and/or increased tolerance to environmental stress (iy-bm)Patent Number: WO2009077611-A2; Date:2009-06-25
- Plants with increased yield (lt)Patent Number: WO2010034672-A1; Date:2010-04-01
- Method for producing a transgenic plant cell, a plant or a part thereof with increased resistance biotic stressPatent Number: WO2010037714-A1; Date:2010-04-08
- Plants with increased yield (nue)Patent Number: WO2010046221-A1; Date:2010-04-29
- RECOMBINANT HOST CELLS COMPRISING PHOSPHOKETOLASESPatent Number: KR1020130117753-A; Date:2013-10-28