Genomes and Genes
Pseudomonas fluorescens Pf-5
Alias: Pseudomonas fluorescens str. Pf-5, Pseudomonas fluorescens strain Pf-5
- Adaptive divergence in experimental populations of Pseudomonas fluorescens. II. Role of the GGDEF regulator WspR in evolution and development of the wrinkly spreader phenotypePatrick Goymer
Department of Plant Sciences, University of Oxford, UK
Genetics 173:515-26. 2006..Despite the proclivity of mutations within wspR to generate WS, sequence analysis of wspR from 53 independently obtained WS showed no evidence of sequence change in this gene...
- Reciprocal regulation of pyoluteorin production with membrane transporter gene expression in Pseudomonas fluorescens Pf-5Marion Brodhagen
Department of Botany and Plant Pathology, Oregon State University, Corvallis, OR 97331, USA
Appl Environ Microbiol 71:6900-9. 2005..This report provides evidence of a coordination of pyoluteorin production and the transcription of genes encoding a linked transport apparatus, wherein each requires the other for optimal expression...
- Exploiting genetic diversity by directed evolution: molecular breeding of type III polyketide synthases improves productivityWenjuan Zha
Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana Champaign, 600 S Mathews Ave, Urbana, IL 61801, USA
Mol Biosyst 4:246-8. 2008....
- The sigma factor sigma s affects antibiotic production and biological control activity of Pseudomonas fluorescens Pf-5A Sarniguet
Institut National de la Recherche Agronomique, Centre de Recherches de Rennes, Le Rheu, France
Proc Natl Acad Sci U S A 92:12255-9. 1995..fluorescens. These results indicate that a sigma factor encoded by rpoS influences antibiotic production, biological control activity, and survival of P. fluorescens on plant surfaces...
- Availability of iron to Pseudomonas fluorescens in rhizosphere and bulk soil evaluated with an ice nucleation reporter geneJ E Loper
Horticultural Crops Research Laboratory, U S Department of Agriculture, Corvallis, Oregon 97330, USA
Appl Environ Microbiol 63:99-105. 1997..We speculate that iron acquisition systems of plants and other rhizosphere organisms may provide available sources of iron to established rhizosphere populations of P. fluorescens...
- A cryptic PKS-NRPS gene locus in the plant commensal Pseudomonas fluorescens Pf-5 codes for the biosynthesis of an antimitotic rhizoxin complexNicole Brendel
Leibniz Institute for Natural Product Research and Infection Biology, HKI, Beutenbergstr 11 a, D 07745 Jena, Germany
Org Biomol Chem 5:2211-3. 2007....
- The genomisotopic approach: a systematic method to isolate products of orphan biosynthetic gene clustersHarald Gross
Center for Marine Biotechnology and Biomedicine, Scripps Institution of Oceanography and Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA
Chem Biol 14:53-63. 2007..Application of the genomisotopic approach to isolate the product of this gene cluster resulted in the discovery of orfamide A, founder of a group of bioactive cyclic lipopeptides...
- Universal external RNA controls for microbial gene expression analysis using microarray and qRT-PCRZ Lewis Liu
National Center for Agricultural Utilization Research USDA ARS, 1815 North University Street, Peoria, IL 61604, USA
J Microbiol Methods 68:486-96. 2007..Application of these controls also improves efficiency and facilitates high throughput applications of gene expression analysis using the qRT-PCR assay...
- Identification and sequence analysis of the genes encoding a polyketide synthase required for pyoluteorin biosynthesis in Pseudomonas fluorescens Pf-5B Nowak-Thompson
USDA ARS, Corvallis, OR 973301, USA
Gene 204:17-24. 1997....
- Crystal structure of halogenase PltA from the pyoluteorin biosynthetic pathwayAllan H Pang
Department of Pharmaceutical Sciences, College of Pharmacy, University of Kentucky, 789 South Limestone Street, Lexington, KY 40536 0596, USA
J Struct Biol 192:349-57. 2015....
- The two-component regulators GacS and GacA influence accumulation of the stationary-phase sigma factor sigmaS and the stress response in Pseudomonas fluorescens Pf-5C A Whistler
Molecular and Cellular Biology Program, Oregon State University, Corvallis, Oregon 97331, USA
J Bacteriol 180:6635-41. 1998..The results of this study provide evidence for the predominant roles of GacS and GacA in the regulatory cascade controlling stress response and antifungal metabolite production in P. fluorescens...
- Characterization of the pyoluteorin biosynthetic gene cluster of Pseudomonas fluorescens Pf-5B Nowak-Thompson
Agricultural Research Service, U S Department of Agriculture, Corvallis, Oregon 97330, USA
J Bacteriol 181:2166-74. 1999..Therefore, PltR is proposed to be a transcriptional activator of linked pyoluteorin biosynthesis genes...
- The sigma factor RpoS is required for stress tolerance and environmental fitness of Pseudomonas fluorescens Pf-5Virginia O Stockwell
Department of Botany and Plant Pathology, Oregon State University, Corvallis, OR 97331, USA
Microbiology 151:3001-9. 2005..RpoS is a key determinant in stress response and environmental fitness of the rhizosphere bacterium P. fluorescens Pf-5...
- Novel lectin-like bacteriocins of biocontrol strain Pseudomonas fluorescens Pf-5Annabel H A Parret
Centre of Microbial and Plant Genetics, Katholieke Universiteit Leuven, Kasteelpark Arenberg 20, B 3001 Heverlee, Belgium
Appl Environ Microbiol 71:5197-207. 2005..Our observations are consistent with earlier observations, which hinted that MMBL-like bacteriocins represent a new family of antibacterial proteins, probably with a novel mode of action...
- Organic acid exudation and pH changes by Gordonia sp. and Pseudomonas fluorescens grown with P adsorbed to goethiteEsther Hoberg
Institute for Applied Botany, University of Hamburg, Ohnhorststrasse 18, 22609 Hamburg, Germany
Microbiol Res 160:177-87. 2005..produced five different organic acids in varying amounts depending on P source and time. In contrast, P. fluorescens exuded mainly citrate and only small amounts of two to three other organic acids irrespective of P source or time...
- Biosynthesis of phloroglucinolJihane Achkar
Department of Chemistry, Michigan State University, East Lansing, Michigan 48824, USA
J Am Chem Soc 127:5332-3. 2005....
- Lon protease influences antibiotic production and UV tolerance of Pseudomonas fluorescens Pf-5C A Whistler
Molecular and Cellular Biology Program, Corvallis, Oregon
Appl Environ Microbiol 66:2718-25. 2000....
- Influence of Nitrogen Source on 2,4-diacetylphloroglucinol Production by the Biocontrol Strain Pf-5Hultberg M
Department of Horticulture, Microbial Horticultural Laboratory, Swedish University of Agricultural Sciences, Alnarp, Sweden
Open Microbiol J 2:74-8. 2008..These results suggest that it is possible to manipulate the nutrient solution to increase the reliability and efficacy of biological control agents...
- Chemical intervention in bacterial lignin degradation pathways: Development of selective inhibitors for intradiol and extradiol catechol dioxygenasesPaul D Sainsbury
Department of Chemistry, University of Warwick, Coventry CV4 7AL, UK
Bioorg Chem 60:102-9. 2015..jostii RHA1, gave rise to 4-carboxymuconolactone on the β-ketoadipate pathway, whereas in Pseudomonas fluorescens Pf-5 disulfiram treatment gave rise to a metabolite found to be glycine betaine aldehyde. ..
- Pseudomonas fluorescens Pf-5 genome-wide mutant screen for resistance to the antimicrobial peptide alfalfa snakin-1Nicolás D Ayub
Instituto de Genética Ewald A Favret CICVyA INTA, De los reseros S N, Castelar C25 1712, Buenos Aires, Argentina Consejo Nacional de Investigaciones Científicas y Técnicas CONICET, Avda Rivadavia 1917, C1033AAJ, Cuidad Autonoma de Buenos Aires, Argentina
FEMS Microbiol Lett 362:1-6. 2015..Collectively, these findings suggest that snakin-1 acts on microbial adhesion properties. ..
- Crystallization and preliminary X-ray diffraction analysis of a putative carbon-carbon bond hydrolase from Mycobacterium abscessus 103Zhang Zhang
MOE Key Laboratory of Cell Activities and Stress Adaptations, School of Life Sciences, Lanzhou University, Lanzhou, Gansu 730000, People s Republic of China
Acta Crystallogr F Struct Biol Commun 71:239-42. 2015..0, b = 63.4, c = 74.7 Å, α = 90.0, β = 103.2, γ = 90.0°. Assuming the presence of two mPhlG molecules in the asymmetric unit, VM was calculated to be 2.5 Å(3) Da(-1), which corresponds to a solvent content of 50%...
- Characterisation of Dyp-type peroxidases from Pseudomonas fluorescens Pf-5: Oxidation of Mn(II) and polymeric lignin by Dyp1BRahman Rahmanpour
Department of Chemistry, University of Warwick, Coventry CV4 7AL, UK
Arch Biochem Biophys 574:93-8. 2015..A mechanism for release of this fragment from lignin oxidation is proposed. ..
- Plant lectin-like antibacterial proteins from phytopathogens Pseudomonas syringae and Xanthomonas citriMaarten G K Ghequire
Centre of Microbial and Plant Genetics, KU Leuven, Kasteelpark Arenberg 20, 3001 Heverlee, Belgium Laboratory of Molecular Immunology, Rega Institute for Medical Research, KU Leuven, 3000 Leuven, Belgium Molecular Recognition Unit, Department of Structural Biology, Vlaams Instituut voor Biotechnologie, 1050 Brussel, Belgium Structural Biology Brussels, Department of Biotechnology DBIT, Vrije Universiteit Brussel, 1050 Brussel, Belgium
Environ Microbiol Rep 4:373-80. 2012....
- The effect of iron limitation on the transcriptome and proteome of Pseudomonas fluorescens Pf-5Chee Kent Lim
Department of Chemistry and Biomolecular Sciences, Macquarie University, Sydney, Australia
PLoS ONE 7:e39139. 2012..Comparison of the transcriptomic data with the proteomic data suggested that iron acquisition is regulated at both the transcriptional and post-transcriptional levels...
- A global regulator of secondary metabolite production in Pseudomonas fluorescens Pf-5N Corbell
Department of Botany and Plant Pathology, Oregon State University, Corvallis 97331, USA
J Bacteriol 177:6230-6. 1995..These results show that apdA encodes a putative sensor kinase component of a classical two-component regulatory system that is required for secondary-metabolite production by P. fluorescens Pf-5...
- Structure-inhibitory activity relationships of pyrrolnitrin analogues on its biosynthesisYoung Soo Keum
Department of Molecular Biotechnology, Konkuk University, Gwangjin gu, Seoul, Republic of Korea
Appl Microbiol Biotechnol 89:781-9. 2011..The results may help more detailed understanding of this unusual enzyme. In addition, the information will be useful for the development of novel fungicide, compatible with pyrrolnitrin-producing bacterium...
- Effects of pesticides on the bacterial production of pyrrolnitrinYoung Soo Keum
Department of Agricultural Biotechnology, Seoul National University, Seoul, Republic of Korea
J Agric Food Chem 58:5531-7. 2010..Pathway specific inhibition by fenpiclonil, fludioxonil, and methimazole will give structural insights of corresponding enzymes...
- Inactivation of the GacA response regulator in Pseudomonas fluorescens Pf-5 has far-reaching transcriptomic consequencesKarl A Hassan
Department of Chemistry and Biomolecular Sciences, Macquarie University, NSW, Australia
Environ Microbiol 12:899-915. 2010..Two novel gene clusters expressed under the control of gacA were identified from transcriptome analysis, and we propose global-regulator-based genome mining as an approach to decipher the secondary metabolome of Pseudomonas spp...
- Mobile genetic elements in the genome of the beneficial rhizobacterium Pseudomonas fluorescens Pf-5Dmitri V Mavrodi
Department of Plant Pathology, Washington State University, Pullman, WA 99164 6430, USA
BMC Microbiol 9:8. 2009..In this study, we analyzed mobile genetic elements of the Pf-5 genome in an effort to identify determinants that might contribute to Pf-5's ability to adapt to changing environmental conditions and/or colonize new ecological niches...
- Positive autoregulation and signaling properties of pyoluteorin, an antibiotic produced by the biological control organism Pseudomonas fluorescens Pf-5Marion Brodhagen
Department of Botany and Plant Pathology, Oregon State University, Corvallis, Oregon 97331, USA
Appl Environ Microbiol 70:1758-66. 2004..These data establish that the polyketide pyoluteorin is an autoregulatory compound and functions as a signal molecule influencing the spectrum of secondary metabolites produced by the bacterial cell...
- Complete genome sequence of the plant commensal Pseudomonas fluorescens Pf-5Ian T Paulsen
The Institute for Genomic Research, Rockville, Maryland, USA
Nat Biotechnol 23:873-8. 2005..In addition to six known secondary metabolites produced by P. fluorescens Pf-5, three novel secondary metabolite biosynthesis gene clusters were also identified that may contribute to the biocontrol properties of P. fluorescens Pf-5...
- Biocontrol traits of Pseudomonas spp. are regulated by phase variationDaan van den Broek
Leiden University, Institute of Biology Leiden, Clusius Laboratory, Wassenaarseweg 64, 2333 AL Leiden, The Netherlands
Mol Plant Microbe Interact 16:1003-12. 2003..Therefore phase variation not only plays a role in escaping animal defense but it also appears to play a much broader and vital role in the ecology of bacteria producing exoenzymes, antibiotics, and other secondary metabolites...
- Characterization of Pseudomonas putida genes responsive to nutrient limitationChris K C Syn
Department of Biological Sciences, National University of Singapore, 14 Science Drive 4, Singapore 117 543
Microbiology 150:1661-9. 2004..Additionally, it was independent of oxygen availability, in contrast to that in Escherichia coli...
- Structural basis for channelling mechanism of a fatty acid beta-oxidation multienzyme complexMomoyo Ishikawa
Biomolecular Engineering Research Institute, Furuedai, Suita, Osaka, Japan
EMBO J 23:2745-54. 2004..This channelling mechanism could be applied to other beta-oxidation multienzymes, as revealed from the homology model of the human mitochondrial trifunctional enzyme complex...
- The DeltafliD mutant of Pseudomonas syringae pv. tabaci, which secretes flagellin monomers, induces a strong hypersensitive reaction (HR) in non-host tomato cellsR Shimizu
Laboratory of Plant Pathology and Genetic Engineering, Faculty of Agriculture, Okayama University, 1 1 1 Tsushima naka, 700 8530 Okayama, Japan
Mol Genet Genomics 269:21-30. 2003..These results, together with our previous study, suggest that the flagellin monomer of pv. tabaci acts as a strong elicitor to induce HR-associated cell death in non-host tomato cells...
- Crystal structure of formaldehyde dehydrogenase from Pseudomonas putida: the structural origin of the tightly bound cofactor in nicotinoprotein dehydrogenasesNobutada Tanaka
School of Pharmaceutical Sciences, Showa University, 1 5 8 Hatanodai, Shinagawa ku, 142 8555, Tokyo, Japan
J Mol Biol 324:519-33. 2002....
- Phenotypic selection and phase variation occur during alfalfa root colonization by Pseudomonas fluorescens F113Maria Sanchez-Contreras
Departamento de Biologia, Universidad Autonoma de Madrid, Madrid, Spain
J Bacteriol 184:1587-96. 2002..Mutation of the F113 sss gene, encoding a site-specific recombinase, showed that most of the phenotypic variation was due to the activity of this recombinase, indicating that phase variation occurs during rhizosphere colonization...
- Interaction of NahR, a LysR-type transcriptional regulator, with the alpha subunit of RNA polymerase in the naphthalene degrading bacterium, Pseudomonas putida NCIB 9816-4Woojun Park
Department of Microbiology, Cornell University, Ithaca, NY 14853 8101, USA
FEMS Microbiol Lett 213:159-65. 2002..Gene activation by NahR is consistent with the general transcriptional mechanism of class I transcription factors, which function by contacting alphaRNAP...
- Regulation of the aprX-lipA operon of Pseudomonas fluorescens B52: differential regulation of the proximal and distal genes, encoding protease and lipase, by ompR-envZConor N McCarthy
School of Health Science, Griffith University, PMB 50 Gold Coast Mail Centre, Gold Coast, QLD 4217, Australia
FEMS Microbiol Lett 241:243-8. 2004..Differences in the regulation of lipase and protease may be related to the proximal and distal locations of aprX and lipA within the operon...
- Lon protease functions as a negative regulator of type III protein secretion in Pseudomonas syringaeJames Bretz
Department of Cell Biology and Molecular Genetics, University of Maryland, College Park, MD 20742, USA
Mol Microbiol 45:397-409. 2002..These results indicate that expression of the hrp regulon and type III secretion are negatively regulated by Lon-mediated degradation of HrpR...
- A two-component hydroxylase involved in the assimilation of 3-hydroxyphenyl acetate in Pseudomonas putidaElsa Arias-Barrau
Departamento de Bioquimica y Biologia Molecular, Facultad de Veterinaria, Universidad de Leon, 24007 Leon, Spain
J Biol Chem 280:26435-47. 2005....
- The nadA gene of Pseudomonas fluorescens PGPR strain 267.1Monika Marek-Kozaczuk
Department of General Microbiology, University of Maria Curie Skłodowska, Akademicka 19 st, Lublin 20 033, Poland
Curr Microbiol 51:122-6. 2005..0 kDa) with substantial identity to various bacterial quinolinate synthetases. The nadA gene complemented quinolinic acid synthesis deficiency and niacin auxotrophy of the P. fluorescens 106 P nadA mutant...
- Phloroglucinol mediates cross-talk between the pyoluteorin and 2,4-diacetylphloroglucinol biosynthetic pathways in Pseudomonas fluorescens Pf-5Teresa A Kidarsa
USDA ARS Horticultural Crops Research Laboratory, Corvallis, OR 97330, USA
Mol Microbiol 81:395-414. 2011....
- Ligand-induced domain rearrangement of fatty acid beta-oxidation multienzyme complexDaisuke Tsuchiya
Biomolecular Engineering Research Institute, 6 2 3 Furuedai, Suita, Osaka 565 0874, Japan
Structure 14:237-46. 2006..The conformational changes suggest the structural events occurring during the chain reaction catalyzed by the multienzyme complex...
- TonB-dependent outer-membrane proteins and siderophore utilization in Pseudomonas fluorescens Pf-5Sierra L Hartney
Department of Botany and Plant Pathology, Oregon State University, 2082 Cordley Hall, Corvallis, OR 97331, USA
Biometals 24:193-213. 2011..The diversity and complexity of the TBDTs and TBDRs with roles in iron uptake clearly indicate the importance of iron in the fitness and survival of Pf-5 in the environment...
- Probing the substrate specificity of aminopyrrolnitrin oxygenase (PrnD) by mutational analysisJung Kul Lee
Department of Chemical and Biomolecular Engineering, University of Illinois, 600 South Mathews Avenue, Urbana, IL 61801, USA
J Bacteriol 188:6179-83. 2006..Interestingly, several of the mutants obtained exhibited higher catalytic efficiency (approximately two- to sevenfold higher) with the physiological substrate aminopyrrolnitrin than the wild-type enzyme exhibited...
- Identification and characterization of a novel nitrilase from Pseudomonas fluorescens Pf-5Jung Soo Kim
Department of Chemical Engineering, Konkuk University, 1 Hwayang dong, Gwangjin gu, Seoul, 143 701, South Korea
Appl Microbiol Biotechnol 83:273-83. 2009..fluorescens NitPf5 useful for industrial applications, including enzymatic synthesis of various cyanocarboxylic acids...
- Mechanism of hcnA mRNA recognition in the Gac/Rsm signal transduction pathway of Pseudomonas fluorescensKarine Lapouge
Département de Microbiologie Fondamentale, Universite de Lausanne, Bâtiment Biophore, CH 1015 Lausanne, Switzerland
Mol Microbiol 66:341-56. 2007..Effective control of hcnA expression by the Gac/Rsm system appears to result from the combination of the three appropriately spaced recognition elements...
- Identification and characterization of the flavin:NADH reductase (PrnF) involved in a novel two-component arylamine oxygenaseJung Kul Lee
University of Illinois at Urbana Champaign, Department of Chemical and Biomolecular Engineering, 600 South Mathews Avenue, Urbana, IL 61801, USA
J Bacteriol 189:8556-63. 2007..This PrnF enzyme represents the first cloned and characterized flavin reductase component in a novel two-component arylamine oxygenase system...
- Isolation and identification of rhizoxin analogs from Pseudomonas fluorescens Pf-5 by using a genomic mining strategyJoyce E Loper
Horticultural Crops Research Laboratory, Agricultural Research Service, United States Department of Agriculture, Corvallis, Oregon 97330, USA
Appl Environ Microbiol 74:3085-93. 2008..This study highlights the value of the genomic sequence of the soil bacterium P. fluorescens Pf-5 in providing leads for the discovery of novel metabolites with significant biological properties...
- DNA sequence of proline permease gene from Pseudomonas fluorescens and predicted structure of proline permeaseH Hosoya
National Institute of Bioscience and Human Technology, Agency of Industrial Science and Technology, Ibaraki, Japan
Biosci Biotechnol Biochem 58:2099-101. 1994..The DNA sequence is 71.0% and 70.7% identical and the amino acid sequence is 75.8% and 76.0% similar to those of Escherichia coli and Salmonella typhimurium, respectively...
- Monoclonal antibody against species-specific epitope of Pseudomonas aeruginosa Hsp60 protein cross-reacts with Pseudomonas stutzeri and other Pseudomonas speciesE Lüneberg
Institut fur Medizinische Mikrobiologie, Medizinische Hochschule Hannover, Germany
FEMS Microbiol Lett 154:131-7. 1997..aeruginosa and all four cross-reacting species constitute a DNA homology group within the rRNA group I of the family Pseudomonadaceae, which belong to the gamma-subclass of the Proteobacteria...
- Four genes from Pseudomonas fluorescens that encode the biosynthesis of pyrrolnitrinP E Hammer
Novartis Crop Protection, Inc, Research Triangle Park, North Carolina 27709, USA
Appl Environ Microbiol 63:2147-54. 1997....
- A chromosomal locus required for copper resistance, competitive fitness, and cytochrome c biogenesis in Pseudomonas fluorescensC H Yang
Department of Plant, Pathology, University of California, Riverside 92521, USA
Proc Natl Acad Sci U S A 93:7315-20. 1996..However, two mutants in cycH were copper-sensitive and oxidase-positive, suggesting that the functions of these genes, rather than cytochrome c oxidase itself, were required for resistance to copper...
- Tn5-directed cloning of pqq genes from Pseudomonas fluorescens CHA0: mutational inactivation of the genes results in overproduction of the antibiotic pyoluteorinU Schnider
Laboratorie de Biologie Microbienne, Universite de Lausanne, Lausanne Dorigny, Switzerland
Appl Environ Microbiol 61:3856-64. 1995..However, the Phl- phenotype of strain CHA625 was due not to the pqq defect but presumably to a secondary mutation. In conclusion, a lack of PQQ markedly stimulates the production of Plt in P. fluorescens...
- Nucleotide sequences of the trpI, trpB, and trpA genes of Pseudomonas syringae: positive control unique to fluorescent pseudomonadsS Auerbach
Department of Biology, University of Iowa, Iowa City 52242
Gene 123:25-32. 1993..Differences in patterns of codon usage distinguish the trpI genes of P. syringae and P. putida from P. aeruginosa trpI and from the trpB and trpA genes of all three species...
- Subunit structure of a class A aspartate transcarbamoylase from Pseudomonas fluorescensS T Bergh
Department of Biochemistry, Wayne State University School of Medicine, Detroit, MI 48201
Proc Natl Acad Sci U S A 90:9818-22. 1993..We propose that the P. fluorescens ATCase is composed of two trimers of 34-kDa catalytic chains and is likely to be a D3 dodecamer with an arrangement of subunits analogous to that of the class B ATCase molecules...
- Transcription of the faoAB operon which encodes the HDT multienzyme complex involved in fatty acid beta-oxidation in Pseudomonas fragi B-0771S Sato
Department of Biology, College of Arts and Sciences, University of Tokyo
J Biochem 115:286-92. 1994..fragi, suggesting that there is a regulatory element interacting with the transcription repressor...
- Relationship of protein structure of isoleucyl-tRNA synthetase with pseudomonic acid resistance of Escherichia coli. A proposed mode of action of pseudomonic acid as an inhibitor of isoleucyl-tRNA synthetaseT Yanagisawa
Department of Molecular Biology, School of Science, Nagoya University, Japan
J Biol Chem 269:24304-9. 1994....
- Molecular cloning of two Pseudomonas flagellin genes and basal body structural genesC Winstanley
Department of Genetics and Microbiology, University of Liverpool, UK
Microbiology 140:2019-31. 1994..The deduced molecular mass of P. putida PaW8 flagellin (68 kDa) differed significantly from its apparent molecular mass estimated by PAGE, possibly as a consequence of post-translational modification.(ABSTRACT TRUNCATED AT 250 WORDS)..
- Amplification of the groESL operon in Pseudomonas putida increases siderophore gene promoter activityV Venturi
Department of Molecular Cell Biology, University of Utrecht, The Netherlands
Mol Gen Genet 245:126-32. 1994..The groESL operon codes for the chaperone proteins GroES and GroEL, which are responsible for mediating the folding and assembly of many proteins...
- Reconstitution, morphology and crystallization of a fatty acid beta-oxidation multienzyme complex from Pseudomonas fragiM Ishikawa
Biomolecular Engineering Research Institute, 6 2 3 Furuedai, Suita, Osaka 565, Japan
Biochem J 328:815-20. 1997..These findings allow the possible location of the interface between the two subunits, which should be crucial for the exhibition of thiolase activity...
- Iron regulation of siderophore biosynthesis and transport in Pseudomonas putida WCS358: involvement of a transcriptional activator and of the Fur proteinV Venturi
Department of Molecular Cell Biology, University of Utrecht, The Netherlands
Mol Microbiol 15:1081-93. 1995..We also report the identification and characterization of the P. putida WCS358 fur gene. The Fur protein of strain WCS358 is structurally and functionally similar to other cloned Fur proteins from other bacterial species...
- Structural comparisons of superoxide dismutasesJ I Harris
Eur J Biochem 106:297-303. 1980..Two histidine residues are conserved in all proteins and secondary structure predictions suggest they are in close proximity in the same alpha-helix. These residues may provide ligands for the bound metal...
- Structure of iron superoxide dismutase from Pseudomonas ovalis at 2.9-A resolutionD Ringe
Proc Natl Acad Sci U S A 80:3879-83. 1983..Crystals of complexes with the inhibitors azide or fluoride are considerably more resistant to irradiation than those of the free enzyme. The structure of the apoprotein is identical to that of the iron-containing molecule...
- [Nucleotide sequence of the rpoC-gene coding for RNA polymerase beta'-subunit of Pseudomonas putida]A V Danilkovich
Dokl Akad Nauk SSSR 303:241-5. 1988
- Isolation of a third lipoamide dehydrogenase from Pseudomonas putidaG Burns
Department of Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center, Oklahoma City 73190
J Bacteriol 171:665-8. 1989..These results suggest that LPD-3 is a lipoamide dehydrogenase associated with an unknown multienzyme complex which can replace LPD-Glc as the E3 component of pyruvate and 2-ketoglutarate dehydrogenases in lpdG mutants...
- Nucleotide sequence of the Pseudomonas fluorescens signal peptidase II gene (lsp) and flanking genesL Isaki
Department of Microbiology, Uniformed Services University of the Health Sciences, Bethesda, Maryland 20889 4799
J Bacteriol 172:6512-7. 1990..fluorescens revealed two highly conserved domains in this enzyme. Furthermore, the five genes which constitute an operon in E. coli and Enterobacter aerogenes were found in P. fluorescens in the same order as in the first two species...
- Cloning and sequence analysis of the LPD-glc structural gene of Pseudomonas putidaJ A Palmer
Department of Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center, Oklahoma City 73190
J Bacteriol 173:3109-16. 1991..This result indicates that lpdG can be expressed separately from the other genes of the operon...
- Cloning and sequencing the urocanase gene (hutU) from Pseudomonas putidaM Fessenmaier
Institute of Organic Chemistry, University of Karlsruhe, Germany
FEBS Lett 286:55-7. 1991..One subunit of urocanase consists of 556 amino acids and has a molecular mass of 60,771 Da...
- Characterization of 4-hydroxyphenylpyruvate dioxygenase. Primary structure of the Pseudomonas enzymeU Ruetschi
Department of Clinical Chemistry, Gothenburg, University, Sahlgren s Hospital, Sweden
Eur J Biochem 205:459-66. 1992..The Tyr and His distributions, and the position of a single Cys residue, all suggest a few likely sites, outside the C-terminal segment, for this centre...
- A set of genes encoding a second toluene efflux system in Pseudomonas putida DOT-T1E is linked to the tod genes for toluene metabolismG Mosqueda
Department of Biochemistry and Molecular Biology of Plants, Estacion Experimental del Zaidin, Consejo Superior de Investigaciones Cientificas, E 18008 Granada, Spain
J Bacteriol 182:937-43. 2000..These results are discussed in the context of the use of multiple efflux pumps involved in solvent tolerance in P. putida DOT-T1E...
- Homology study of two polyhydroxyalkanoate (PHA) synthases from Pseudomonas aureofaciensF Umeda
Graduate School of Pharmaceutical Sciences, Osaka University, Suita, Japan
DNA Seq 12:281-4. 2001..Furthermore, multialignment of PHA synthase AA sequences implied that both enzymes of PHA synthase 1 and PHA synthase 2 were highly conserved in the four strains including P. aureofaciens...
- Functional analysis of alkane hydroxylases from gram-negative and gram-positive bacteriaTheo H M Smits
Institute of Biotechnology, Swiss Federal Institute of Technology, ETH Honggerberg, CH 8093 Zurich, Switzerland
J Bacteriol 184:1733-42. 2002..fluorescens CHA0, which is no longer able to grow on C(12) to C(16) alkanes. All alkane hydroxylase homologs, except the Acinetobacter sp. ADP1 AlkM, allowed at least one of the three hosts to grow on n-alkanes...
- Homology of the root adhesin of Pseudomonas fluorescens OE 28.3 with porin F of P. aeruginosa and P. syringaeR De Mot
F A Janssens Laboratory of Genetics, Catholic University of Leuven, Heverlee, Belgium
Mol Gen Genet 231:489-93. 1992..However, a cysteine-rich domain present in the OprFs of P. aeruginosa and P. syringae is absent from the adhesin of P. fluorescens. Instead, it contains a shorter sequence with eight alternating proline residues...
- Cloning, sequencing, and expression of the cold-inducible hutU gene from the antarctic psychrotrophic bacterium Pseudomonas syringaeKamala L Janiyani
Centre for Cellular and Molecular Biology, Hyderabad 500 007, India
Appl Environ Microbiol 68:1-10. 2002..The psychrotrophic enzyme, however, has an extended N-terminal end...
- Cloning, sequence and crystallographic structure of recombinant iron superoxide dismutase from Pseudomonas ovalisC J Bond
Program in Biomolecular Structure and Design, University of Washington, USA
Acta Crystallogr D Biol Crystallogr 56:1359-66. 2000..The gene was subcloned and the recombinant protein overexpressed, purified and crystallized in a trigonal space group. The structure was determined by molecular replacement and was refined to 2.1 A resolution...
- Identification of a novel bacterial sequence associated with Crohn's diseaseC L Sutton
Department of Pathology and Laboratory Medicine, University of California, Los Angeles, California, USA
Gastroenterology 119:23-31. 2000..In this study, representational difference analysis (RDA) was used to isolate DNA segments preferentially abundant in lamina propria mononuclear cells of lesional mucosa vs. adjacent uninvolved mucosa...
- Genes expressed in Pseudomonas putida during colonization of a plant-pathogenic fungusS W Lee
Department of Plant Pathology, University of California, Riverside, California 92521 0122, USA
Appl Environ Microbiol 66:2764-72. 2000..The location of the inducible promoter of two clones suggested that DAGK and the ABC transporter are induced and may play a role in colonization of the fungus P. parasitica by P. putida 06909...
- Multicopy suppression of a gacA mutation by the infC operon in Pseudomonas fluorescens CHA0: competition with the global translational regulator RsmAC Blumer
Laboratoire de Biologie Microbienne, Universite de Lausanne, CH 1015, Lausanne, Switzerland
FEMS Microbiol Lett 187:53-8. 2000..This finding suggests that some product(s) of the infC operon can compete with RsmA at the level of translation in P. fluorescens CHA0 and that important biocontrol traits can be regulated at this level...
- Structural, kinetic, and calorimetric characterization of the cold-active phosphoglycerate kinase from the antarctic Pseudomonas sp. TACII18M Bentahir
Laboratoire de Biochimie, Institut de Chimie B6, Universite de Liege, Sart Tilman, B 4000 Liege, Belgium
J Biol Chem 275:11147-53. 2000..It is proposed that the heat-labile domain acts as a destabilizing domain, providing the required flexibility around the active site for catalysis at low temperatures...
- Adaptive divergence in experimental populations of Pseudomonas fluorescens. I. Genetic and phenotypic bases of wrinkly spreader fitnessAndrew J Spiers
Department of Plant Sciences, University of Oxford, Oxford OX1 3RB, United Kingdom
Genetics 161:33-46. 2002..Analysis of >30 kb of DNA encoding traits required for expression of the WS phenotype, including a regulatory locus, has not revealed the mutational causes, indicating a complex genotype-phenotype map...
- Conservation of the pyrrolnitrin biosynthetic gene cluster among six pyrrolnitrin-producing strainsP E Hammer
Novartis Agribusiness Biotechnology Research, P O Box 12257, Research Triangle Park, NC 27709, USA
FEMS Microbiol Lett 180:39-44. 1999..The prnA gene from M. fulvus is about 45% similar to prnA from the other strains and contains regions which are highly conserved among all six strains...
- Novel biodegradable aromatic plastics from a bacterial source. Genetic and biochemical studies on a route of the phenylacetyl-coa catabolonB Garcia
Departamento de Bioquimica y Biologia Molecular, Facultades de Biologia y Veterinaria, Campus de Vegazana s n, Universidad de Leon, 24007 Leon, Espana
J Biol Chem 274:29228-41. 1999..The analysis of the organization and function of the pha locus and its relationship with the core of the phenylacetyl-CoA catabolon is reported and discussed...
- Crystal structure of Pseudomonas fluorescens 4-hydroxyphenylpyruvate dioxygenase: an enzyme involved in the tyrosine degradation pathwayL Serre
Institut de Biologie Structurale Jean Pierre Ebel, CNRS CEA, Grenoble, France
Structure 7:977-88. 1999..Moreover, in humans, a HPPD deficiency is found to be related to tyrosinemia, a rare hereditary disorder of tyrosine catabolism...
- Crystal structure of 2-oxoisovalerate and dehydrogenase and the architecture of 2-oxo acid dehydrogenase multienzyme complexesA Aevarsson
Departments of Biological Structure and Biochemistry, Biomolecular Structure Center, University of Washington, P O Box 357742, Seattle, Washington 98195 7742, USA
Nat Struct Biol 6:785-92. 1999..The E1 structure, together with previous structural information on E2 and E3, completes the picture of the shared architectural features of these enormous macromolecular assemblies...
- DNA polymerase C of the thermophilic bacterium Thermus aquaticus: classification and phylogenetic analysis of the family C DNA polymerasesY P Huang
Department of Microbiology and Immunology, College of Medicine, The University of Arizona, Tucson, AZ 85724, USA
J Mol Evol 48:756-69. 1999..Third, the cyanobacterial family C DNA pol, classified as class III because it is encoded by a split gene, forms a group with the high-G+C Gram-positive bacteria...
- Investigation of the function of proteins associated to polyhydroxyalkanoate inclusions in Pseudomonas putida BMO1H E Valentin
Department of Biology, James Madison University, Harrisonburg, VA, USA
J Biotechnol 64:145-57. 1998..Gas-chromatographic analysis of the cellular dry mass revealed that the mutant strains accumulated 30-50% less PHA than the P. putida wild type...
- The requirement of RpoN (sigma factor sigma54) in denitrification by Pseudomonas stutzeri is indirect and restricted to the reduction of nitrite and nitric oxideE Hartig
Lehrstuhl für Mikrobiologie der Universität Karlsruhe, D 76128 Karlsruhe, Germany
Appl Environ Microbiol 64:3092-5. 1998....
- Isolation and characterization of toluene-sensitive mutants from the toluene-resistant bacterium Pseudomonas putida GM73K Kim
Department of Microbiology, Gyeongsang National University, Gazwadong 900, Chinju 660 701, Korea
J Bacteriol 180:3692-6. 1998..Characterization of these mutants and identification of mutant genes suggested that active efflux mechanism and efficient repair of damaged membranes were important in toluene resistance...
- A two-component system plays an important role in the root-colonizing ability of Pseudomonas fluorescens strain WCS365L C Dekkers
Leiden University, Institute of Molecular Plant Sciences, Clusius Laboratory, The Netherlands
Mol Plant Microbe Interact 11:45-56. 1998..We conclude that colonization is an active process in which an environmental stimulus, through this two-component system, activates a so far unknown trait that is crucial for colonization...
- Amino acid sequence of iron-superoxide dismutase from Pseudomonas ovalisT Isobe
Department of Chemistry, Faculty of Science, Tokyo Metropolitan University, Japan
FEBS Lett 223:92-6. 1987..The sequence information of the P. ovalis dismutase will facilitate refinement of the X-ray crystallographic data that are now available at 2.9 A resolution...
- Tryptophan 7-halogenase (PrnA) structure suggests a mechanism for regioselective chlorinationChangjiang Dong
Centre for Biomolecular Sciences, EaStCHEM, University of St Andrews, St Andrews KY16 9ST, UK
Science 309:2216-9. 2005..The resulting HOCl is guided through the tunnel to tryptophan, where it is activated to participate in electrophilic aromatic substitution...
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